Publications by authors named "Joseph Adongo Awuni"

4 Publications

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Detection of avian influenza virus: a comparative study of the in silico and in vitro performances of current RT-qPCR assays.

Sci Rep 2020 05 21;10(1):8441. Epub 2020 May 21.

Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università, 10, Legnaro, Padova, 35020, Italy.

Avian influenza viruses (AIV) are negative sense RNA viruses posing a major threat to the poultry industry worldwide, with the potential to spread to mammals, including humans; hence, an accurate and rapid AIV diagnosis is essential. To date AIV detection relies on molecular methods, mainly RT-qPCR directed against AIV M gene segment. The evolution of AIV represents a relevant issue in diagnostic RT-qPCR due to possible mispriming and/or probe-binding failures resulting in false negative results. Consequently, RT-qPCR for AIV detection should be periodically re-assessed both in silico and in vitro. To this end, a specific workflow was developed to evaluate in silico the complementarity of primers and probes of four published RT-qPCR protocols to their target regions. The four assays and one commercially available kit for AIV detection were evaluated both for their analytical sensitivity using eight different viral dilution panels and for their diagnostic performances against clinical specimens of known infectious status. Differences were observed among the tests under evaluation, both in terms of analytical sensitivity and of diagnostic performances. This finding confirms the importance of continuously monitoring the primers and probes complementarity to their binding regions.
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http://dx.doi.org/10.1038/s41598-020-64003-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242438PMC
May 2020

Disentangling the role of Africa in the global spread of H5 highly pathogenic avian influenza.

Nat Commun 2019 11 22;10(1):5310. Epub 2019 Nov 22.

Department of Comparative Biomedical Sciences, Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.

The role of Africa in the dynamics of the global spread of a zoonotic and economically-important virus, such as the highly pathogenic avian influenza (HPAI) H5Nx of the Gs/GD lineage, remains unexplored. Here we characterise the spatiotemporal patterns of virus diffusion during three HPAI H5Nx intercontinental epidemic waves and demonstrate that Africa mainly acted as an ecological sink of the HPAI H5Nx viruses. A joint analysis of host dynamics and continuous spatial diffusion indicates that poultry trade as well as wild bird migrations have contributed to the virus spreading into Africa, with West Africa acting as a crucial hotspot for virus introduction and dissemination into the continent. We demonstrate varying paths of avian influenza incursions into Africa as well as virus spread within Africa over time, which reveal that virus expansion is a complex phenomenon, shaped by an intricate interplay between avian host ecology, virus characteristics and environmental variables.
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http://dx.doi.org/10.1038/s41467-019-13287-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874648PMC
November 2019

Genetically Different Highly Pathogenic Avian Influenza A(H5N1) Viruses in West Africa, 2015.

Emerg Infect Dis 2016 12 15;22(12):2132-2136. Epub 2016 Dec 15.

To trace the evolution of highly pathogenic influenza A(H5N1) virus in West Africa, we sequenced genomes of 43 viruses collected during 2015 from poultry and wild birds in 5 countries. We found 2 co-circulating genetic groups within clade 2.3.2.1c. Mutations that may increase adaptation to mammals raise concern over possible risk for humans.
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http://dx.doi.org/10.3201/eid2212.160578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5189143PMC
December 2016

Impact of avian influenza on village poultry production globally.

Ecohealth 2014 18;11(1):63-72. Epub 2013 Oct 18.

Faculty of Veterinary Science, University of Sydney, 425 Werombi Road, Camden, NSW, 2570, Australia,

Village poultry and their owners were frequently implicated in disease transmission in the early days of the highly pathogenic avian influenza (HPAI) H5N1 pandemic. With improved understanding of the epidemiology of the disease, it was recognized that village poultry raised under extensive conditions pose less of a threat than intensively raised poultry of homogeneous genetic stock with poor biosecurity. This paper provides an overview of village poultry production and the multiple ways that the HPAI H5N1 pandemic has impacted on village poultry, their owners, and the traders whose livelihoods are intimately linked to these birds. It reviews impact in terms of gender and cultural issues; food security; village poultry value chains; approaches to biosecurity; marketing; poultry disease prevention and control; compensation; genetic diversity; poultry as part of livelihood strategies; and effective communication. It concludes on a positive note that there is growing awareness amongst animal health providers of the importance of facilitating culturally sensitive dialogue to develop HPAI prevention and control options.
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http://dx.doi.org/10.1007/s10393-013-0867-xDOI Listing
February 2015