Publications by authors named "José Luiz Carneiro da Rocha"

5 Publications

  • Page 1 of 1

Evaluation of Biological Activity of in Experimental Models.

Iran J Pharm Res 2019 ;18(2):793-802

Departamento de Saúde, Universidade Estadual de Feira de Santana, Feira de Santana, Bahia, Brasil.

The plants of the genus (Polygalaceae) are employed in folk medicine for the treatment of several pathologies, including disorders of the bowel and kidney, as anesthetic, expectorant and anti-inflammatory. The present study was undertaken to investigate the antiedematogenic and antinociceptive activities of methanolic extract of A. W. Benn (MEPB) in mice. The antinociceptive activity of MEPB was evaluated using the writhing, formalin, and tail immersion tests. The carrageenan-induced paw edema test was used to assess the antiedematogenic activity of MEPB. Mice motor performance was evaluated in the rota rod and open field tests and the acute toxicity were evaluated over 14 days. High-performance liquid chromatography was used to determine the fingerprint chromatogram of MEPB. Oral administration of MEPB (75- 600 mg/kg) reduced the number of writhing induced by acetic acid. In the formalin test, the oral pre-treatment with MEPB (75 - 600 mg/kg) produced a dose-related inhibition only of the late phase. MEPB (300 and 600 mg/kg) reduced the carrageenan-induced paw edema. In contrast, the treatment with MEPB (300 and 600 mg/kg) did not prevent the thermal nociception in the tail immersion test. MEPB (600 mg/kg)-treated mice did not show any motor performance alterations. Over the study duration of 14 days, there were no mortality or toxic signs recorded in the group mice given 6000 mg/kg of MEPB. The present study demonstrated, for the first time, the antinociceptive and antiedematogenic properties of .
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http://dx.doi.org/10.22037/ijpr.2019.1100655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706750PMC
January 2019

Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction.

PLoS One 2018 4;13(12):e0207676. Epub 2018 Dec 4.

Federal University of Bahia, Salvador, Bahia, Brazil.

Propolis is a natural product with many demonstrated biological activities and propolis extract has been used in the food, pharmaceutical and cosmetics industries. Different works have showed the variations in the chemical composition, and consequently, on the biological activity of the propolis that are associated with its type and geographic origin. Due to this study evaluated propolis extracts obtained through supercritical extraction and ethanolic extraction (conventional) in three samples of different types of propolis (red, green and brown), collected from different regions in Brazil (state of Bahia). Analyses were performed to determine the humidity, water activity, the content of total ash, proteins, lipids and fiber in raw propolis samples. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH), catechin, ferulic acid and luteolin and antimicrobial activity against two bacteria (Staphylococcus aureus and Escherichia coli) were determined for all extracts. For the green and red ethanolic extracts the anti-leishmanicidal potential was also evaluated. The physicochemical profiles showed agreement in relation to the literature. The results identified significant differences among the extracts (p>0.05), which are in conformity with their extraction method, as well as with type and botanical origin of the samples. The extraction with supercritical fluid was not efficient to obtain extracts with the highest contents of antioxidants compounds, when compared with the ethanolic extracts. The best results were shown for the extracts obtained through the conventional extraction method (ethanolic) indicating a higher selectivity for the extraction of antioxidants compounds. The red variety showed the largest biological potential, which included the content of antioxidants compounds. The results found in this study confirm the influence of the type of the raw material on the composition and characteristics of the extracts. The parameters analysis were important to characterize and evaluate the quality of the different Brazilian propolis extracts based on the increased use of propolis by the natural products industry.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207676PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279037PMC
May 2019

Asemeia ovata (Polygalaceae): Quantitative determination and evaluation in silico of identified substances by HPLC-DAD.

Comput Biol Chem 2018 Aug 30;75:65-73. Epub 2018 Apr 30.

Departamento de Saúde, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, Novo Horizonte, CEP: 44036-900, Bahia, Brazil. Electronic address:

Background: In Brazil, the Asemeia genus has 19 species (12 endemic) and 2 varieties (both endemic) and some of them are found in semi-arid Bahia.

Objective: The objective of this study was to quantitatively determine identified substances by HPLC-DAD in Asemeia ovata extracts and to predict their biological activities in silico.

Method: The quantification method by HPLC-DAD has been validated according to the guidelines of the International Conference of Harmonization. The prediction in silico activities was made by Target Fishing methods (TF), followed by docking by the program DOCK 6.7 and assessment of interaction profiles for Protein-Ligand Interaction Profiler server.

Results: It was possible to identify and quantify using HPLC-DAD substances: rutin, luteolin-7-O-glucoside, caffeic acid, p-coumaric acid and trans-ferulic acid. The ChemProt 2.0 server was selected for TF method, which has shown potential activity of compounds on molecular targets such as Carbonic anhydrase 12, epidermal growth factor receptor and sodium-glucose cotransporter 2.

Conclusion: This work provides new results for the species both from a biological and chemical point of view, and has interesting potential to be discovered with the prospect of further studies.
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http://dx.doi.org/10.1016/j.compbiolchem.2018.04.018DOI Listing
August 2018

Chemical Composition and Biological Activity of Extracts Obtained by Supercritical Extraction and Ethanolic Extraction of Brown, Green and Red Propolis Derived from Different Geographic Regions in Brazil.

PLoS One 2016 8;11(1):e0145954. Epub 2016 Jan 8.

Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil.

The variations in the chemical composition, and consequently, on the biological activity of the propolis, are associated with its type and geographic origin. Considering this fact, this study evaluated propolis extracts obtained by supercritical extraction (SCO2) and ethanolic extraction (EtOH), in eight samples of different types of propolis (red, green and brown), collected from different regions in Brazil. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH and ABTS), Artepillin C, p-coumaric acid and antimicrobial activity against two bacteria were determined for all extracts. For the EtOH extracts, the anti-proliferative activity regarding the cell lines of B16F10, were also evaluated. Amongst the samples evaluated, the red propolis from the Brazilian Northeast (states of Sergipe and Alagoas) showed the higher biological potential, as well as the larger content of antioxidant compounds. The best results were shown for the extracts obtained through the conventional extraction method (EtOH). However, the highest concentrations of Artepillin C and p-coumaric acid were identified in the extracts from SCO2, indicating a higher selectivity for the extraction of these compounds. It was verified that the composition and biological activity of the Brazilian propolis vary significantly, depending on the type of sample and geographical area of collection.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145954PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706314PMC
July 2016

Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent.

PLoS One 2015 7;10(8):e0134489. Epub 2015 Aug 7.

Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil.

The aim of this study was to determine the best processing conditions to extract Brazilian green propolis using a supercritical extraction technology. For this purpose, the influence of different parameters was evaluated such as S/F (solvent mass in relation to solute mass), percentage of co-solvent (1 and 2% ethanol), temperature (40 and 50°C) and pressure (250, 350 and 400 bar) using supercritical carbon dioxide. The Global Yield Isotherms (GYIs) were obtained through the evaluation of the yield, and the chemical composition of the extracts was also obtained in relation to the total phenolic compounds, flavonoids, antioxidant activity and 3,5-diprenyl-4-hydroxicinnamic acid (Artepillin C) and acid 4-hydroxycinnamic (p-coumaric acid). The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110. These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process. Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134489PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529176PMC
May 2016
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