Publications by authors named "José Luis Sánchez-Huerta"

11 Publications

  • Page 1 of 1

Amino acid changes in HA and determinants of pathogenicity associated with influenza virus A H1N1pdm09 during the winter seasons 2015-2016 and 2016-2017 in Mexico.

Virus Res 2019 10 21;272:197731. Epub 2019 Aug 21.

Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico.

Biennial H1N1pdm09 influenza A virus (IAV) epidemics have been associated with major severity of respiratory disease in Mexico. Atypically and in contrast with what happened in USA, Canada and Europe during 2017, an increase of infections due to the H1N1pdm09 pandemic virus instead of H3N2 was observed. In order to determine the viral contribution to severe acute respiratory disease, we characterized the pathogenicity determinants of IAV in Mexico during the 2015-2016 and 2016-2017 seasons. The RNA segments of 20 IAV samples were sequenced by NGS platform and phylogenetic analysis was conducted. The analysis of the hemagglutinin (HA) sequences established that all virus samples, except one, belong to clade (6B.1). The IAVs presented the substitution S162 N, which introduces a new glycosylation site in the hemagglutinin. We also found the D222 G substitution, which has been associated with a higher tropism towards the lower respiratory tract, and a non-reported insertion of one Ile in NS1 (Ile113). The IAVs from 2016 to 2017 in Mexico belong to the new clade 6B.1. The new glycosylation site in HA (S162 N) is a major change that may affect the efficacy of the current vaccine. We detected in several patients pathogenicity determinants associated with the severity of the respiratory disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.virusres.2019.197731DOI Listing
October 2019

Healthcare-Associated Pneumonia: Don't Forget About Respiratory Viruses!

Front Pediatr 2019 16;7:168. Epub 2019 May 16.

Epidemiology Department, Federico Gómez Children's Hospital of Mexico, Mexico City, Mexico.

Healthcare-associated infections are an important cause of morbidity and mortality, are among the most common adverse events in healthcare, and of them, pneumonia is the most commonly reported. Our objective was to evaluate the incidence and clinical outcome of respiratory viruses in hospital-acquired pneumonia (HAP). This was a prospective cohort study, include patients aged between 0 and 18 who fulfilled Centers for Diseases Control and Prevention (CDC) criteria for HAP. Demographic and clinical data were obtained, and a nasopharyngeal swab specimen was taken for the detection of respiratory viruses. All included patients were monitored until discharge to collect data on the need for mechanical ventilation, intensive care unit (ICU) admission, and mortality. All-cause 30-day mortality was also ascertained. Four thousand three hundred twenty-seven patients were followed for 42,658 patient-days and 5,150 ventilator-days. Eighty-eight patients (2.03%) met the CDC criteria for HAP, 63 patients were included, and clinical and epidemiological characteristics showed no statistically significant differences between patients with virus associated healthcare-associated pneumonia (VAHAP) and those with non-viral healthcare-associated pneumonia (NVHAP). At least one respiratory virus was detected in 65% [95% CI (53-77)] of episodes of HAP, with a single viral pathogen observed in 53.9% and coinfection with 2 viruses in 11.1% of cases. The outcome in terms of ICU admission, mechanical ventilation and the 30-day mortality did not show a significant difference between groups. In two-thirds of the patients a respiratory virus was identified. There was no difference in mortality or the rest of the clinical outcome variables. About half of the patients required mechanical ventilation and 10% died, which emphasizes the importance of considering these pathogens in nosocomial infections, since their identification can influence the decrease in hospital costs and be taken into account in infection control policies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2019.00168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532533PMC
May 2019

The Onthophagus fuscus (Coleoptera: Scarabaeidae) species complex: an update and the description of a new species.

Zootaxa 2019 Feb 12;4555(2):151-186. Epub 2019 Feb 12.

Instituto de Ecología, A.C., Carretera antigua a Coatepec 351, El Haya, 91070, Xalapa, Veracruz, Mexico..

The Onthophagus fuscus Boucomont, 1932 (Coleoptera: Scarabaeidae: Scarabaeinae: Onthophagini) species complex has a total of eight species, one of which is a new species (Onthophagus orizabensis new species) and three are elevated from the subspecies level (O. canescens Zunino Halffter, 1988 new rank; O. mycetorum Zunino Halffter, 1988 new rank; O. parafuscus Zunino Halffter, 2005 new rank). Onthophagus navarretorum Delgado Capistan, 1996 is included, while Onthophagus semiopacus has been removed from the species complex. The distribution map and key for the identification of species in this species complex are updated. Habitat preferences, ecological tolerance, and the restriction of this species complex to the mountains of the Mexican Transition Zone are discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.11646/zootaxa.4555.2.1DOI Listing
February 2019

Simultaneous Detection of Beta and Gamma Human Herpesviruses by Multiplex qPCR Reveals Simple Infection and Coinfection Episodes Increasing Risk for Graft Rejection in Solid Organ Transplantation.

Viruses 2018 12 19;10(12). Epub 2018 Dec 19.

Research Unit in Virology and Cancer, Children's Hospital of Mexico Federico Gómez, 06720 Mexico City, Mexico.

Herpesviruses are common components of the human microbiome that become clinically relevant when a competent immunosurveillance is compromised, such as in transplantation. Members of the beta and gamma subfamilies are associated with a wide diversity of pathologies, including end-organ disease and cancer. In this study, we developed a multiplex qPCR technique with high specificity, sensitivity, efficiency and predictability that allowed the simultaneous detection and quantification of beta and gamma human herpesviruses. The technique was tested in a cohort of 34 kidney- or liver-transplanted pediatric patients followed up for up to 12 months post-transplant. Viral load was determined in 495 leukocyte-plasma paired samples collected bi-weekly or monthly. Human herpesvirus (HHV) 7 was the herpesvirus most frequently found in positive samples (39%), followed by Epstein-Barr virus (EBV) (20%). Also, EBV and HHV7 were present in the majority of coinfection episodes (62%). The share of positive samples exclusively detected either in leukocytes or plasma was 85%, suggesting that these herpesviruses tended to take a latent or lytic path in an exclusive manner. Infection by human cytomegalovirus (HCMV) and HHV6, as well as coinfection by EBV/HHV7 and EBV/HHV6/HHV7, were associated with graft rejection (RR = 40.33 ( = 0.0013), 5.60 ( = 0.03), 5.60 ( = 0.03) and 17.64 ( = 0.0003), respectively). The routine monitoring of beta and gamma herpesviruses should be mandatory in transplant centers to implement preventive strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/v10120730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316002PMC
December 2018

Two new species of with remarks on ecology, distributions, and evolutionary relationships (Coleoptera, Scarabaeidae, Scarabaeinae).

Zookeys 2018 29(747):71-86. Epub 2018 Mar 29.

Instituto de Ecología, A.C., Carretera antigua a Coatepec 351, El Haya, Xalapa, Veracruz, 91070, Mexico.

Two new species of the genus Weber are described from the region of Los Chimalapas, Oaxaca, Mexico: and A diagnosis for distinguishing these new species from the other species of this genus in North America is included. This paper is illustrated with pictures of the dorsal habitus and the male genitalia of the new species. The evolutionary relationships of the species are discussed, as well as their distribution and ecology. It is considered that the species of the genus present in North and Central America correspond to the Typical Neotropical and Mountain Mesoamerican distribution patterns.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/zookeys.747.22731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904362PMC
March 2018

Surveillance for the identification of cases of acute respiratory infection by enterovirus D68 in children in a tertiary level care hospital during 2014-2016.

Bol Med Hosp Infant Mex 2018 ;75(1):23-30

Hospital Infantil de México Federico Gómez. Secretaría de Salud, Ciudad de México, México.

Background: The reemergence of enterovirus D68 (EV-D68) infections in the United States was reported from August-October 2014 (691 cases). In Mexico, an outbreak at the National Institute of Respiratory Diseases was reported (24 cases). The results of epidemiological surveillance of Enterovirus sp. (EV) and other respiratory viruses in a national pediatric tertiary care level hospital are presented.

Methods: Following the alert issued by the reemergence of EV-D68 in 2014, epidemiological surveillance -which only detected respiratory viruses by PCR in patients with influenza-like illness using nasopharyngeal swabs- expanded to include children with asthma exacerbation or acute respiratory distress. Positive samples to EV were confirmed and typed by sequencing. Subsequent sequencing was used to obtain the complete viral genome.

Results: Of 1705 samples, 13 were positive to EV. Patients with EV presented the following comorbidities: chronic lung disease (7.7%), neoplastic disease (15.4%), allergic asthma/rhinitis (23%), recurrent pneumonia (23%), and other (23%). Of the 13 samples positive for EV, three were positive for EV-D68. These cases required invasive mechanical ventilation, presented no neurological involvement and survived.

Conclusions: The impact of the population studied by EV-D68 was lower than that reported in Mexico during the same period. Cases of EV-D68 infection had multiple comorbidities, but few pulmonary comorbidities, which could explain the low attack rate. The epidemiological surveillance and infection prevention system may have contained the outbreak.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.24875/BMHIM.M18000002DOI Listing
August 2019

Influenza-like illness in healthcare personnel at a paediatric referral hospital: Clinical picture and impact of the disease.

Influenza Other Respir Viruses 2018 07 19;12(4):475-481. Epub 2018 Apr 19.

Hospital Infantil de México Federico Gómez, Ciudad de México, México.

Introduction: Healthcare personnel (HP) are frequently exposed to influenza and can be a source of transmission to patients and other workers, resulting in high-cost outbreaks for healthcare institutions.

Objectives: To analyse the presentation of HP with influenza-like illness (ILI) and the differences between individuals with influenza confirmed by polymerase chain reaction (PCR) and those with a negative test. The secondary objective was to evaluate the duration of symptomatology and work absenteeism as well as the vaccination rate of HP at a paediatric referral hospital.

Methods: A cross-sectional, descriptive study was conducted at a paediatric referral hospital. Clinical and epidemiological data on HP with ILI were collected between January and April 2016. Nasopharyngeal swab for influenza PCR was obtained from one in every three workers with ILI. Telephone follow-up was conducted to document duration of symptoms, complications and absenteeism.

Results: A total of 164 ILI episodes were evaluated in 162 HP. A swab was obtained in 59 cases, and influenza was detected in 30 cases. The clinical picture of HP with confirmed influenza was similar to that of HP with a negative PCR. Arthralgia was more common in those with influenza (90% vs 58%), with a tendency towards statistical significance. No HP required hospitalization, and 78.5% were absent from work at least 1 day.

Conclusions: Influenza causes significant morbidity and absenteeism among HP. Influenza infection was confirmed in only half of HP with an ILI on whom a PCR was performed, suggesting that other respiratory viruses can cause a similar pattern.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/irv.12553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005608PMC
July 2018

Two new species of the species group (Coleoptera, Scarabaeidae, Scarabaeinae).

Zookeys 2017 26(702):113-135. Epub 2017 Sep 26.

Instituto de Ecología, A.C., Carretera antigua a Coatepec 351, El Haya, Xalapa, Veracruz, 91070, Mexico.

is described from the coniferous-oak forests in the state of Guerrero, and from coniferous-oak forests and cloud forests in Jalisco and Nayarit. The new species are closely related to and respectively. Morphological trait combination, geographic distribution, and trophic habits show important differences among the studied species. A distribution map and an updated key to separate the species are included.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3897/zookeys.702.14728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5673967PMC
September 2017

Correlation between viral load of cytomegalovirus and tacrolimus and sirolimus levels in transplanted pediatric patients.

Bol Med Hosp Infant Mex 2016 Jan - Feb;73(1):4-9. Epub 2016 Feb 26.

Subdirección de Servicios Auxiliares de Diagnóstico, Hospital Infantil de México Federico Gómez, México, D.F., México. Electronic address:

Introduction: Survival of transplant patients and grafts depends largely on the use of immunosuppressive drugs. However, a balance remains to be established among immunosuppression, transplant rejection and cytomegalovirus (CMV) infection, which results in a high rate of morbidity and mortality. The aim of this study was to define a better strategy for monitoring transplanted patients based on the analysis of the blood concentration of sirolimus and tacrolimus and the burden of CMV.

Methods: Fifty five post-transplant (kidney and liver) pediatric patients, nine treated with sirolimus and 46 treated with tacrolimus, were included. A total of 541 measurements were obtained. In each measurement the concentration of immunosuppressant in whole blood and CMV viral load in plasma and whole blood was quantified by real-time PCR. Pearson correlation coefficient (r) was estimated.

Results: Values of r ≤0.0747 were found for the relationship between dose and concentration of immunosuppressant; r = 0.9406 for the relationship between viral load in whole blood and plasma, and r ≤0.4616 for the relationship between concentration of immunosuppressant and viral load.

Conclusions: These data support that the doses of immunosuppressive drugs do not correlate with the levels of the same in whole blood. Therefore, systemic levels of immunosuppressant should be constantly monitored together with CMV load. Meanwhile, a high correlation between viral load measured in whole blood and plasma was found.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bmhimx.2015.12.006DOI Listing
February 2016

[Frequency of respiratory viruses and clinical characteristics in children attending a care center in Mexico City].

Salud Publica Mex 2010 Nov-Dec;52(6):528-32

Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, México, México.

Objective: To describe the frequency of respiratory viruses and clinical characteristics in children with respiratory signs and symptoms in a tertiary care center in Mexico.

Material And Methods: Patients with a clinical diagnosis of respiratory infection and a positive immunofluorescence result (Light Diagnostics) from January 2004 to October 2006 were included.

Results: From the 986 nashopharyngeal samples, 138 (14%) were positive by immunofluorescence. The frequency was: 80% RSV, 8% parainfluenza 1, 5% parainfluenza 3, 2% adenovirus, 2% influenza A, 1% parainfluenza 2 and 1% influenza B.

Conclusions: Respiratory viruses were detected in 14% of samples tested. RSV was the most frequently identified virus and was associated with pneumonia and bronchiolitis in children younger than 3 years old.
View Article and Find Full Text PDF

Download full-text PDF

Source
June 2011