Publications by authors named "Joon Ho Lee"

123 Publications

Transcranial direct current stimulation decreased cognition-related reaction time in older adults: A systematic review and meta-analysis.

Ageing Res Rev 2021 Jun 2;70:101377. Epub 2021 Jun 2.

Department of Human Movement Science, Incheon National University, Incheon, South Korea; Division of Sport Science, Incheon National University, Incheon, South Korea; Sport Science Institute & Health Promotion Center, Incheon National University, Incheon, South Korea; Neuromechanical Rehabilitation Research Laboratory, Incheon National University, Incheon, South Korea. Electronic address:

Background: This systematic review and meta-analysis investigated the effects of transcranial direct current stimulation (tDCS) on the cognitive functions of healthy older adults by focusing on the changes in reaction time during cognitive tasks.

Method: A total of 31 studies qualified for this meta-analysis, and we acquired 36 comparisons from the included studies for data synthesis. The individual effect sizes were calculated by comparing the altered reaction time during the performance of a specific cognitive task between the active tDCS and sham groups. In two moderator variable analyses, we examined the potentially different effects of the tDCS protocols on the cognition-related reaction time based on the tDCS protocol used (i.e., online vs. offline tDCS) and the five cognitive domains: (a) perceptual-motor function, (b) learning and memory, (c) executive function / complex attention, (d) language, and (e) social cognition. Meta-regression analyses were conducted to estimate the relationship between demographic and tDCS parameter characteristics and the changes in reaction time.

Results: The random-effects model meta-analysis revealed significant small effects of tDCS on cognition-related reaction time. Specifically, providing online tDCS significantly reduced the reaction time, and these patterns were observed during learning and memory and executive function / complex attention tasks. However, applying offline tDCS failed to find any significant reduction of reaction time across various cognitive tasks. The meta-regression analysis revealed that the effects of tDCS on the reaction time during the performance of cognitive tasks increased for the older people.

Conclusions: These findings suggest that providing online tDCS may effectively improve the ageing-induced reaction time related to specific cognitive functions of elderly people.
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http://dx.doi.org/10.1016/j.arr.2021.101377DOI Listing
June 2021

Insecticide resistance trait may contribute to genetic cluster change in Bemisia tabaci MED (Hemiptera: Aleyrodidae) as a potential driving force.

Pest Manag Sci 2021 Jul 24;77(7):3581-3587. Epub 2021 Apr 24.

Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.

Background: Previously, we reported that the majority of the Bemisia tabaci Mediterranean (MED) populations converged from two dominant genetic clusters (cluster 1 and 2) to one (cluster 2) during 1 year in greenhouse tomatoes in Korea. To find possible mechanisms for this phenomenon, we investigated the concurrent changes in resistance traits of the two clusters for three insecticide classes (organophosphate, pyrethroid, and neonicotinoid).

Results: Since the resistance mutation frequencies in regional samples were either high (i.e. the voltage-sensitive sodium channel L925I/T929V mutations and the F392 acetylcholinesterase 1 mutation) or zero (the nicotinic acetylcholine receptor R81T mutation), no meaningful correlation between the resistance allele frequency and genetic cluster was deduced. However, the actual resistance levels to all three insecticide classes were significantly higher in cluster 2 than in cluster 1, suggesting that cluster 2 has a higher resistance potential. Furthermore, thiamethoxam treatment to the mixed population of clusters 1 and 2 over three generations exhibited a strong tendency of population change from cluster 1 to cluster 2.

Conclusion: Our results demonstrated that the insecticide resistance trait is one of the driving forces for rapid genetic cluster change in B. tabaci MED populations. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6412DOI Listing
July 2021

A novel task-specific dystonia: running-induced cervical dystonia in a triathlete.

Neurol Sci 2021 Mar 31. Epub 2021 Mar 31.

Department of Neurology, Ajou University School of Medicine, 5 San, Woncheon-dong, Yongtong-gu, World cup ro Suwon-si, Kyunggi-do, 442-749, South Korea.

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http://dx.doi.org/10.1007/s10072-021-05224-zDOI Listing
March 2021

Comparison of life history parameters of two different genetic clusters of Bemisia tabaci MED (Hemiptera: Aleyrodidae) through single and cross mating.

PLoS One 2021 26;16(3):e0248819. Epub 2021 Mar 26.

Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.

Bemisia tabaci Mediterranean (Gennadius) (Hemiptera: Aleyrodidae) is an economically important insect pest worldwide. Previously, we have reported that most B. tabaci Mediterranean (MED) populations occurring in greenhouse tomatoes in Korea have been displaced from well-differentiated two genetic clusters (C1 and C2) to one (C2) during one-year period. To elucidate factors responsible for this phenomenon, we compared life history parameters of these two different genetic clusters through single and cross mating experiments on two different host plants, cucumber and tobacco, at 26°C. Intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (Ro) were significantly higher in the dominating cluster (C2) (0.247, 1.280, and 192.402, respectively on cucumber; 0.226, 1.253, and 133.792, respectively on tobacco) than in the other cluster (C1) (0.149, 1.161, and 50.539, respectively on cucumber; 0.145, 1.156, and 53.332, respectively on tobacco). Overall performances of cross mating groups, C2fC1m (C2 female × C1 male) and C1fC2m (C1 female × C2 male), were in-between those of C2 and C1, with C2fC1m performing better than C1fC2m. Thus, maternal inheritance appeared to be significantly associated with their life history parameters, with partial involvement of paternal inheritance. Our results demonstrated that the rapid displacement of genetic clusters of B. tabaci MED populations was clearly associated with differences in their life history parameters.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248819PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997046PMC
March 2021

Diagnostic accuracy of Raman spectroscopy for prostate cancer: a systematic review and meta-analysis.

Transl Androl Urol 2021 Feb;10(2):574-583

Department of Urology, Soonchunhyang University Seoul Hospital, Seoul, Korea.

Background: Although various studies have been conducted to demonstrate the possibility of Raman spectroscopy (RS) as a diagnostic tool for prostate cancer (PC), it is difficult to use it in the real clinical area because of imitations in various research processes. Therefore, we did a systematic review and meta-analysis about the accuracy in diagnostic use of RS for PC.

Methods: A literature search was done using PubMed, Embase, and Cochrane library databases in March 2019 to analyze the accuracy of RS for diagnosis of PC. The accuracy of RS for diagnosis of PC was evaluated by means of pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and summary receiver operating characteristic (SROC).

Results: Five studies were included for qualitative analysis by screening the remaining articles according to the inclusion and exclusion criteria by means of a systematic review. The pooled sensitivity and specificity of RS were 0.89 (95% CI: 0.87-0.91) and 0.91 (95% CI: 0.89-0.93), respectively. The overall PLR and NLR were 9.12 (95% CI: 4.15-20.08) and 0.14 (95% CI: 0.07-0.29), respectively. The DOR of RS demonstrated high accuracy (73.32; 95% CI: 18.43-291.73). The area under the curves (AUCs) of SROC curves was 0.93.

Conclusions: RS is an optical diagnostic method with high potential for diagnosis and grading of PC and has advantages of real-time and convenient use. In order to consider real-time use of RS in an actual clinical setting, more studies for standardization and generalization of RS performance and analytical method must be conducted.
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http://dx.doi.org/10.21037/tau-20-924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947438PMC
February 2021

UV-LED lights enhance the establishment and biological control efficacy of Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae).

PLoS One 2021 7;16(1):e0245165. Epub 2021 Jan 7.

Department of Agricultural Biotechnology, Entomology Program, Seoul National University, Seoul, Republic of Korea.

The zoophytophagous mirid Nesidiocoris tenuis (Hemiptera: Miridae) is one of the biological control agents against the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae), a major pest of greenhouse crops. The successful establishment of a biological control agent and its co-occurrence with the target pests increases the efficacy of biological control programs in greenhouses. In this study, we explored the effects of different wavelengths of LED light on establishment of N. tenuis in laboratory condition, with the goal of enhancing the biological control of B. tabaci in greenhouse crops. Nesidiocoris tenuis was most strongly attracted by LED light at a wavelength of 385 nm. This same wavelength was also highly attractive to B. tabaci in Y-tube experiments with lights of specific wavelengths provided is each arm of the apparatus. In trials in growth chambers, we verified the attraction of N. tenuis to 385 nm wavelength. When LED light at a wavelength of 385 nm was used in a growth chamber for 6 hours out of 24 hours, it significantly increased the remaining number of N. tenuis in growth chamber and level of predation compared to treatment with white LED light or without LED light. In conclusion, UV-LED light at a wavelength of 385 nm attracts both B. tabaci and N. tenuis. Thus, it would be used for enhancing early establishment of this mirid bug, better spatial congruence of both mirid bug and whitefly, and better control of the whitefly.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245165PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790406PMC
May 2021

Ultrasound-guided pulsed radiofrequency treatment for distal suprascapular neuropathy: A case report.

Medicine (Baltimore) 2020 Sep;99(39):e22469

Department of Anesthesiology and Pain Medicine, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon, Republic of Korea.

Rationale: Suprascapular neuropathy is a rare cause of shoulder pain, and patients usually presents with posterosuperior shoulder pain and weakness on forward flexion and external rotation. Suprascapular neuropathy associated with rotator cuff pathology has received attention as an emerging cause of this condition. Suprascapular nerve (SSN) block can be used in these patients, and pulsed radio frequency (PRF) can be applied to achieve a long-term effect. Several studies have reported on PRF treatment of the SSN for shoulder pain, but most applied treatment to the nerve trunk under the transverse scapular ligament. This report describes a patient with suprascapular neuropathy treated with selective application of PRF to the distal SSN under ultrasound guidance.

Patient Concerns: A 68-year-old woman suffered from right posterior shoulder pain after traumatic full thickness rotator cuff tear. Her pain was not diminished despite of 2 surgeries.

Diagnoses: She was diagnosed with entrapment of the distal SSN in the spino-glenoid (SGN) notch and suprascapular neuropathy.

Interventions: She underwent surgery to decompress the entrapped SSN in the SGN. After that, we applied PRF on the distal SSN under ultrasound guidance for persistent pain. This treatment was repeated 3 times.

Outcomes: PRF treatment resulted in a slight reduction in the visual analogue scale (VAS) pain score from 7-8/10 to 5-6/10 at the 2 weeks follow-up, and to 2-3/10 at the 1 month follow-up. The reduction in pain was maintained at the 1 year follow-up.

Lessons: PRF treatment of the SSN is typically approached from the main branch in the suprascapular notch. We selectively applied PRF to the distal SSN close to the SGN. This technique was safe and effective.
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http://dx.doi.org/10.1097/MD.0000000000022469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523776PMC
September 2020

Imidazole-Selective Alkyne Hydroamination under Physiological Conditions.

Org Lett 2020 10 23;22(19):7588-7593. Epub 2020 Sep 23.

Center for New Directions in Organic Synthesis, Department of Chemistry, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea.

Imidazole-selective intermolecular hydroamination reaction has been discovered. This unprecedented additive-free addition reaction proceeds in an exclusively regioselective and stereoselective manner with high atom economy under extremely mild reaction conditions.
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http://dx.doi.org/10.1021/acs.orglett.0c02785DOI Listing
October 2020

Effects of online-bandwidth visual feedback on unilateral force control capabilities.

PLoS One 2020 17;15(9):e0238367. Epub 2020 Sep 17.

Department of Human Movement Science, Incheon National University, Incheon, South Korea.

Purpose: The purpose of this study was to examine how different threshold ranges of online-bandwidth visual feedback influence unilateral force control capabilities in healthy young women.

Methods: Twenty-five right-handed young women (mean±standard deviation age = 23.6±1.5 years) participated in this study. Participants unilaterally executed hand-grip force control tasks with their dominant and non-dominant hands, respectively. Each participant completed four experimental blocks in a different order of block presentation for each hand condition: (a) 10% of maximum voluntary contraction (MVC) with ±5% bandwidth threshold range (BTR), (b) 10% of MVC with ±10% BTR, (c) 40% of MVC with ±5% BTR, and (d) 40% of MVC with ±10% BTR. Outcome measures on force control capabilities included: (a) force accuracy, (b) force variability, (c) force regularity, and (d) the number of times and duration out of BTR.

Results: The non-dominant hand showed significant improvements in force control capabilities, as indicated by higher force accuracy, less force variability, and decreased force regularity from ±10% BTR to ±5% BTR during higher targeted force level task. For both hands, the number of times and duration out of BTR increased from ±10% BTR to ±5% BTR.

Conclusions: The current findings suggested that the narrow threshold range of online-bandwidth visual feedback effectively revealed transient improvements in unilateral isometric force control capabilities during higher targeted force level tasks.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238367PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498075PMC
October 2020

A case of one-lung ventilation using a single-lumen tube placed under fiberoptic bronchoscopic guidance in a 4-year-old child: A case report.

Medicine (Baltimore) 2020 Aug;99(34):e21737

Department of Anesthesiology and Pain Medicine, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, 170, Jomaru-ro, Bucheon-si, Gyeonggi-do, Republic of Korea.

Rationale: One-lung ventilation (OLV) is essential for adequate visualization and exposure of the surgical site via a videoscopic approach. Although many instruments facilitating OLV are available, the choice is limited in pediatric patients.

Patient Concerns: A 4-year-old female (weight: 18.6 kg, height: 100 cm) was admitted via our pediatric outpatient clinic because of recurrent hemoptysis, 2 weeks in duration. She had no medical or surgical history.

Diagnosis: Contrast-enhanced computed tomography (CT) revealed a 4.5-cm-diameter mass in the left, lower lung lobe. She was diagnosed with a congenital pulmonary airway malformation (CPAM).

Interventions: She was scheduled for emergency lobectomy via video-assisted thoracoscopic surgery (VATS). To ensure successful VATS, OLV was essential. As our hospital lacked a small-diameter fiberoptic bronchoscope and a proper bronchial blocker, we decided to use single-lumen tube (SLT) with adult fiberoptic bronchoscope.

Outcomes: We performed successful bronchoscopic-guided OLV using a SLT. We aligned the tube to the right upper lobar bronchus and Murphy eye to prevent obstruction of the right upper lobe bronchus. At the end of surgery, the endotracheal tube lumen had been narrowed by blood clots, we decided to exchange the tracheal tube. The tube was immediately exchanged. After re-intubation, the pulse oximetry (SpO2) then gradually increased.

Lessons: Appropriate preparation and careful management should be considered to perform OLV in pediatric patients without significant complications.
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http://dx.doi.org/10.1097/MD.0000000000021737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447431PMC
August 2020

Egg Hatching and First Instar Falling Models of (Hemiptera: Flatidae).

Insects 2020 Jun 3;11(6). Epub 2020 Jun 3.

Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.

Since the citrus flatid planthopper, (Say), was introduced in Korea and many European countries, it has caused serious damage to various agricultural crops and landscape plants. hibernates as eggs beneath the bark and in cracks of tree branches, and then substantial numbers of the first instar nymphs fall from the trees and move to other host plants. Knowing the timing of egg hatching and falling of the first instar nymphs would be key for controlling . In this study, the hatching of overwintered eggs and falling of the first instar nymphs from trees were monitored in several areas of Korea. These data were modeled with two starting points for degree-day accumulation, 1 January and 18 March, with a lower development threshold of 10.1 °C. The egg hatching and first instar falling models both used 1 January because the starting point performed better. The 50% appearance and falling times of the first instar nymphs were predicted to be 360.50 DD and 452.23 DD from 1 January, respectively, indicating that newly hatched nymphs stayed on the trees for about a week (i.e., 91.74 DD). Using these models, changes in the population density of the first instar nymphs of on the trees were simulated, and the optimal control time range targeting the nymphs on the trees was deduced. The control time for nymphs on ground plants bordering the trees was suggested by the first instar falling model, along with observations of population density on the ground plants.
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http://dx.doi.org/10.3390/insects11060345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349211PMC
June 2020

The risk of preterm birth in vanishing twin: A multicenter prospective cohort study.

PLoS One 2020 29;15(5):e0233097. Epub 2020 May 29.

Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea.

Objective: To evaluate not only the risk of total preterm birth (PTB) but also spontaneous preterm birth (sPTB) and indicated preterm birth (iPTB) in vanishing twin (VT).

Study Design: This is a secondary analysis of a multicenter prospective cohort study. In 12 different healthcare institutions, women with singleton pregnancies were enrolled in early pregnancy and followed up till delivery.

Results: A total of 4,746 women were included in the final analysis, and. the frequency of VT was 1.1% (54/4746). VT group had a higher risk for total PTB (PTB<34 weeks, 2.1% vs. 14.8%, p<0.001; PTB<32 weeks, 1.6% vs. 13.0%, p<0.001; PTB<28 weeks, 0.9% vs. 13.0%, p<0.001) than singleton group. The VT group had increased risk for both sPTB and iPTB (<34 weeks, <32 weeks, and <28 weeks), and this increased risk for sPTB and iPTB in VT group remained significant even after controlling for confounders such as maternal age, parity, pre-pregnancy BMI, and mode of conception.

Conclusion: Vanishing twin can be an independent risk factor for both sPTB and iPTB when compared with singleton pregnancy.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233097PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259660PMC
August 2020

Comprehensive pharmacogenomic characterization of gastric cancer.

Genome Med 2020 02 18;12(1):17. Epub 2020 Feb 18.

Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

Background: Gastric cancer is among the most lethal human malignancies. Previous studies have identified molecular aberrations that constitute dynamic biological networks and genomic complexities of gastric tumors. However, the clinical translation of molecular-guided targeted therapy is hampered by challenges. Notably, solid tumors often harbor multiple genetic alterations, complicating the development of effective treatments.

Methods: To address such challenges, we established a comprehensive dataset of molecularly annotated patient derivatives coupled with pharmacological profiles for 60 targeted agents to explore dynamic pharmacogenomic interactions in gastric cancers.

Results: We identified lineage-specific drug sensitivities based on histopathological and molecular subclassification, including substantial sensitivities toward VEGFR and EGFR inhibition therapies in diffuse- and signet ring-type gastric tumors, respectively. We identified potential therapeutic opportunities for WNT pathway inhibitors in ALK-mutant tumors, a significant association between PIK3CA-E542K mutation and AZD5363 response, and transcriptome expression of RNF11 as a potential predictor of response to gefitinib.

Conclusions: Collectively, our results demonstrate the feasibility of drug screening combined with tumor molecular characterization to facilitate personalized therapeutic regimens for gastric tumors.
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http://dx.doi.org/10.1186/s13073-020-0717-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029441PMC
February 2020

Posterior lumbar interbody fusion graft penetrated the lumbar thecal sac in a patient with rheumatoid arthritis: A case report.

Int J Surg Case Rep 2020 23;67:21-24. Epub 2020 Jan 23.

Department of Orthopaedic Surgery, Seoul Spine Institute, Sanggyepaik Hospital, College of Medicine, Inje University, Republic of Korea.

Introduction: Intradural foreign bodies have been reported to be associated with disc material, tumors, and bullets following spinal gunshot injuries. In this report, we describe a case of non-union with minor trauma that caused interbody bone graft material to migrate into the intrathecal area in a patient with RA.

Presentation Of Case: We present the case of a 65-year-old woman visited an outpatient clinic of our hospital after experiencing progressive lower extremity weakness, and voiding and defecation difficulty after fell down several times in the past. She had a history of two spinal decompression with fixation surgeries due to spinal stenosis with a herniated intervertebral disc. She was prescribed steroids and methotrexate for the RA. The results of MRI and CT demonstrated an intradural bone graft material migration with cauda equina syndrome after revision lumbar stenosis surgery. Calcified material protruded to the intracanal area and compressed the cauda equina fiber. After the removal of fragments operation, she recovered from cauda equina symptoms. A follow-up examination two years postoperatively revealed clinical resolution of cauda equina symptoms and a return to partial walking with a cane.

Discussion: The patient had a minor or major trauma, such as a fall, after the revision surgery. After that trauma, the patient presented with some dural injury, kyphotic position, or non-union state causing the dural penetration of the interbody fusion material.

Conclusion: The first report describing displaced PLIF graft material that penetrated the dural sac and caused cauda equina symptoms in a patient with RA. Establishing strategies to minimize these complications is indicated when treating degenerative lumbar spine conditions in patients with RA.
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http://dx.doi.org/10.1016/j.ijscr.2020.01.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994728PMC
January 2020

The opioid epidemic and crisis in US: how about Korea?

Authors:
Joon-Ho Lee

Korean J Pain 2019 Oct;32(4):243-244

Department of Anesthesiology and Pain Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.

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http://dx.doi.org/10.3344/kjp.2019.32.4.243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813894PMC
October 2019

Functional Balance and Postural Control Improvements in Patients With Stroke After Noninvasive Brain Stimulation: A Meta-analysis.

Arch Phys Med Rehabil 2020 01 27;101(1):141-153. Epub 2019 Sep 27.

Department of Human Movement Science, Incheon National University, Incheon, South Korea; Division of Health and Kinesiology, Incheon National University, Incheon, South Korea.

Objectives: The postural imbalance poststroke limits individuals' walking abilities as well as increase the risk of falling. We investigated the short-term treatment effects of noninvasive brain stimulation (NIBS) on functional balance and postural control in patients with stroke.

Data Sources: We started the search via PubMed and the Institute for Scientific Information's Web of Science on March 1, 2019 and concluded the search on April 30, 2019.

Study Selection: The meta-analysis included studies that used either repetitive transcranial magnetic stimulation (rTMS) or transcranial direct current stimulation (tDCS) for the recovery of functional balance and postural control poststroke. All included studies used either randomized controlled trial or crossover designs with a sham control group.

Data Extraction: Three researchers independently performed data extraction and assessing methodological quality and publication bias. We calculated overall and individual effect sizes using random effects meta-analysis models.

Data Synthesis: The random effects meta-analysis model on the 18 qualified studies identified the significant positive effects relating to NIBS in terms of functional balance and postural control poststroke. The moderator-variable analyses revealed that these treatment effects were only significant in rTMS across patients with acute, subacute, and chronic stroke whereas tDCS did not show any significant therapeutic effects. The meta-regression analysis showed that a higher number of rTMS sessions was significantly associated with more improvements in functional balance and postural control poststroke.

Conclusions: Our systematic review and meta-analysis confirmed that NIBS may be an effective option for restoring functional balance and postural control for patients with stroke.
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http://dx.doi.org/10.1016/j.apmr.2019.09.003DOI Listing
January 2020

Population Genetic Structure of Glover (Hemiptera: Aphididae) in Korea.

Insects 2019 Sep 26;10(10). Epub 2019 Sep 26.

Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.

Glover (Hemiptera: Aphididae) is a serious polyphagous agricultural pest worldwide. In the present study, we used eight microsatellite markers to investigate the genetic structure and diversity of populations in Korea. Samples were collected from 37 locations in Korea (18 populations in 2016, 14 populations in 2017, and five populations in 2018) from pepper plants. had low to moderate genetic diversity, and expected heterozygosity () ranged from 0.354 to 0.719. A Mantel test of isolation by distance indicated no relationship between genetic structure and geographic distance among all populations ( = 0.0004, = 0.370), suggesting high gene flow among populations in Korea. Populations of in Korea were divided into two distinct genetic clusters (Δ = 2). In 2016 and 2017, the genetic clusters changed into opposite genetic structures within one year mostly in northwest and southeast parts of Korea. Possible relevance of study results was discussed. Chemical control, cyclical parthenogenesis, and immigrants from the exterior might have resulted in this low genetic diversity and opposite genetic clusters.
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http://dx.doi.org/10.3390/insects10100319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835795PMC
September 2019

Population genetic structure of Bemisia tabaci MED (Hemiptera: Aleyrodidae) in Korea.

PLoS One 2019 25;14(7):e0220327. Epub 2019 Jul 25.

Entomology Program, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.

The sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a major agricultural pest that causes economic damages worldwide. In particular, B. tabaci MED (Mediterranean) has resulted in serious economic losses in tomato production of Korea. In this study, 1,145 B. tabaci MED females from 35 tomato greenhouses in different geographic regions were collected from 2016 to 2018 (17 populations in 2016, 13 in 2017, and five in 2018) and analyzed to investigate their population genetic structures using eight microsatellite markers. The average number of alleles per population (NA) ranged from 2.000 to 5.875, the expected heterozygosity (HE) ranged from 0.218 to 0.600, the observed heterozygosity (HO) ranged from 0.061 to 0.580, and the fixation index inbreeding coefficient (FIS) ranged from -0.391 to 0.872 over the three years of the study. Some significant correlation (p < 0.05) was present between genetic differentiations (FST) and geographical distance, and a comparatively high proportion of variation was found among the B. tabaci MED populations. The B. tabaci MED populations were divided into two well-differentiated genetic clusters within different geographic regions. Interestingly, its genetic structures converged into one genetic cluster during just one year. The reasons for this genetic change were speculated to arise from different fitness, insecticide resistance, and insect movement by human activities.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0220327PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6657892PMC
March 2020

A novel decellularized skeletal muscle-derived ECM scaffolding system for in situ muscle regeneration.

Methods 2020 01 3;171:77-85. Epub 2019 Jul 3.

Wake Forest Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA. Electronic address:

The cell-based tissue engineering strategies have gained attention in restoring normal tissue function after skeletal muscle injuries; however, these approaches require a donor tissue biopsy and extensive cell expansion process prior to implantation. In order to avoid this limitation, we developed a novel cell-free muscle-specific scaffolding system that consisted of a skeletal muscle-derived decellularized extracellular matrix (dECM) and a myogenic factor, insulin growth factor-1 (IGF-1). Rheological, morphological, and biological properties of this muscle-specific scaffold (IGF-1/dECM) as well as collagen and dECM scaffolds were examined. The cell viability in all scaffolds had over 90% at 1, 3, and 7 days in culture. The cell proliferation in the IGF-1/dECM was significantly increased when compared with other groups. More importantly, the IGF-1/dECM strongly supported the myogenic differentiation in the scaffold as confirmed by myosin heavy chain (MHC) immunofluorescence. We also investigated the feasibility in a rabbit tibialis anterior (TA) muscle defect model. The IGF-1/dECM had a significantly greater number of myofibers when compared to both collagen and dECM groups at 1 and 2 months after implantation. We demonstrated that this novel muscle-specific scaffolding system could effectively promote the muscle tissue regeneration in situ.
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http://dx.doi.org/10.1016/j.ymeth.2019.06.027DOI Listing
January 2020

Prognostic Impact of Microsatellite Instability in Asian Gastric Cancer Patients Enrolled in the ARTIST Trial.

Oncology 2019 2;97(1):38-43. Epub 2019 May 2.

Department of Medical Oncology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

Background: Caucasian patients with microsatellite instability (MSI)-high gastric cancer (GC) may have better prognosis but worse outcomes.

Objective: Here we explored the prognostic role of MSI in Asian patients.

Methods: This post hoc analysis comprehended radically resected GC patients randomized to XP (capecitabine/cisplatin) or XPRT. MSI status was assessed by combining immunohistochemistry with multiplex polymerase chain reaction. The MSI prognostic effect on disease-free survival (DFS) and overall survival (OS) was evaluated.

Results: 393 tissue samples were analyzed and 35 (9%) were MSI-high. This subgroup was characterized by: older age, Borrmann classification 1-2, antral localization, T3-4 stage, and intestinal type. At univariable analysis, the microsatellite-stable subgroup showed a trend toward a worse prognosis as compared to the MSI-high group: 3-year DFS was 76.3 versus 85.4% (p = 0.122); 3-year OS was 81.7 versus 91.4% (p = 0.046). Multivariable analyses confirmed it in both DFS (hazard ratio, HR = 2.32 [95% CI 0.91, 5.88]; p = 0.077) and OS (HR = 3.17 [95% CI 0.97, 10.43]; p = 0.057).

Conclusions: MSI-high status was associated with specific clinical-pathological features and a trend toward better outcomes of Asian GC patients.
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http://dx.doi.org/10.1159/000499628DOI Listing
July 2019

Cesarean section does not increase the prevalence of allergic disease within 3 years of age in the offsprings.

Obstet Gynecol Sci 2019 Jan 27;62(1):11-18. Epub 2018 Nov 27.

Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Seoul, Korea.

Objective: In this study, we evaluated the prevalence of allergic disease in offsprings delivered via the delivery modes of vaginal delivery vs. planned Cesarean section vs. Cesarean section with labor.

Methods: This study included 175 mother-neonate pairs from Severance Hospital who were enrolled in the Cohort for Childhood Origin of Asthma and allergic diseases study. Information regarding prenatal environmental factors, delivery, and diagnosis of allergic diseases was obtained from a questionnaire and medical record review. Patients with at least 3 years of follow-up data were included in this study. Results were adjusted for sex, birth weight, gestational age at birth, season of birth, neonatal intensive care unit admission, parity, breastfeeding, and maternal factors.

Results: A total of 175 offsprings were eligible for analysis. Among the subjects, 52.0% were delivered by vaginal delivery, 34.3% by planned Cesarean section, and 16.6% by Cesarean section with labor. Fifty-nine offsprings (33.7%) were diagnosed with allergic disease at a median age of 1 year (range 0.5-3 years). The prevalence of allergic disease was not associated with delivery mode after adjusting for confounding variables. Time period from membrane rupture to delivery, duration of the active phase, and the beginning of the pelvic division prior to Cesarean section were not associated with allergic disease development in offsprings.

Conclusion: Cesarean section, irrespective of the occurrence of labor before surgery, did not increase the prevalence of allergic disease in infants up to 3 years of age.
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http://dx.doi.org/10.5468/ogs.2019.62.1.11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333761PMC
January 2019

H-Bonding Mediated Asymmetric Intramolecular Diels-Alder Reaction in the Formal Synthesis of (+)-Aplykurodinone-1.

Org Lett 2018 11 7;20(22):7312-7316. Epub 2018 Nov 7.

Center for New Directions in Organic Synthesis, Department of Chemistry , Hanyang University , 222 Wangshimni-ro , Seongdong-gu, Seoul 04763 , Korea.

An asymmetric formal total synthesis of (+)-aplykurodinone-1 was achieved using a route, in which hydrogen bonding serves as a stereochemical control element governing the π-facial selectivity of intramolecular Diels-Alder (IMDA) reaction of an enone tethered 2-pyrone. In the IMDA process, the configuration at a stereogenic, hydroxyl bearing an α-carbon in the enone dienophile is conveyed in a highly effective manner through intramolecular hydrogen bonding with the enone carbonyl oxygen. The tricyclic lactone, generated in this process, was successfully converted to a late stage intermediate in Danishefsky's synthesis of aplykurodinone-1.
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http://dx.doi.org/10.1021/acs.orglett.8b03250DOI Listing
November 2018

Anterograde irrigation - assisted ureteroscopic lithotripsy in patients with percutaneous nephrostomy.

Int Braz J Urol 2019 Mar-Apr;45(2):406-407

St. Vincent's Hospital, College of Medicine, The Catholic University of Korea.

In complicated urinary tract infection with ureteral calculi, urinary diversion is inevitable. So, stenting or percutaneous drainage can be an option. In hemodynamically unstable patients, percutaneous drainage is superior to ureteral stenting (1). Once acute infection is controlled, definite treatment of the stone is necessary. According to a guideline, semirigid ureteroscopy is recommended for lower and mid - ureter stone and flexible ureteroscopy for upper ureter stone (2). Semi - rigid ureteroscopy can migrate stone to kidney, especially in upper ureter stone, lowering stone free rate (3). Not only flexible ureteroscopy creates additional costs but also is barely available in developing countries (4, 5). So, the authors would like to introduce anterograde irrigation - assisted ureteroscopic lithotripsy in patients with percutaneous nephrostomy. Retrograde irrigation was connected and flowed minimally enough to secure visual field. Once stone is noted, another saline irrigation, which is placed above 40 cm over the patient is connected to nephrostomy. Retrograde irrigation is disconnected from ureteroscope and the previous connected channel on ureteroscope is opened. Actual pressure detected by barometer from the opened channel of ureteroscope is usually about 30 cmH2O while anterograde irrigation is administered in maximal flow, which means fully opened anterograde irrigation is not hazardous to kidney. There was no complication in 17 patients submitted to this method. Video shows advantages of our practice: clear visual field; reduced risk of stone migration into kidney; induced spontaneous passage of fragments without using instrumentation; and decreased operation time. In short, most of surgeons, even unexperienced, can perform an excellent procedure with less time consuming using our method.
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http://dx.doi.org/10.1590/S1677-5538.IBJU.2018.0238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541133PMC
July 2019

Analysis of caudal epidurogram in single center: A preliminary study of lumbar radiculopathy management.

Medicine (Baltimore) 2018 Oct;97(41):e12810

Department of Anesthesiology and Pain Medicine, Soonchunhyang University Bucheon hospital, Soonchunhyang University College of Medicine, Gyeonggi-do, Republic of Korea.

A caudal epidural block involves placing a needle through the sacral hiatus and delivering medication into the epidural space. The procedure is safe and simple, but failure rates can be as high as 25%. The purpose of this study was to investigate the success rate of caudal epidural block by analyzing needle placement and dye flow pattern.We retrospectively analyzed the medical records of patients who underwent caudal epidural block under spinal stenosis. A case was defined as a failure if it met at least one of the following four criteria: the epidural needle was not placed correctly inside the caudal canal; blood regurgitation or aspiration in the needle was observed; the contrast dye was injected into a blood vessel; or a large amount of the dye leaked into the sacral foramen or did not reach the L5-S1 level.At least 1 failure criterion was observed in 14 cases (17.7%), while none of the failure criteria were satisfied in 65 successful cases (82.3%).No matter how experienced the anesthesiologist may be, delivery of adequate therapeutic agent is not achieved in approximately 20% of cases. Therefore, we recommend fluoroscopy-guided needle placement and confirmation by radio-contrast epidurograpy as the best choice.
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http://dx.doi.org/10.1097/MD.0000000000012810DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6203536PMC
October 2018

CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system.

Nucleic Acids Res 2019 01;47(1):e1

Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.

Existing methods to enrich target regions of genomic DNA based on PCR, hybridization capture, or molecular inversion probes have various drawbacks, including long experiment times and low throughput and/or enrichment quality. We developed CRISPR-Cap, a simple and scalable CRISPR-based method to enrich target regions of dsDNA, requiring only two short experimental procedures that can be completed within two hours. We used CRISPR-Cap to enrich 10 target genes 355.7-fold on average from Escherichia coli genomic DNA with a maximum on-target ratio of 81% and high enrichment uniformity. We also used CRISPR-Cap to measure gene copy numbers and detect rare alleles with frequencies as low as 1%. Finally, we enriched coding sequence regions of 20 genes from the human genome. We envision that CRISPR-Cap can be used as an alternative to other widely used target-enrichment methods, which will broaden the scope of CRISPR applications to the field of target enrichment field.
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http://dx.doi.org/10.1093/nar/gky820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326800PMC
January 2019

Selective targeting of KRAS oncogenic alleles by CRISPR/Cas9 inhibits proliferation of cancer cells.

Sci Rep 2018 08 8;8(1):11879. Epub 2018 Aug 8.

Department of Chemistry, Yonsei University, Seoul, Korea.

Mutations within the KRAS oncogene are associated with the proliferation of various cancers. Therapeutic approaches for treating cancers with such mutations have focused on targeting the downstream protein effectors of KRAS. However, to date, no approved treatment has targeted the mutated KRAS oncogene directly. Presently, we used the selectivity of the CRISPR/Cas9 system to directly target mutated KRAS alleles. We designed single-guide RNAs (sgRNAs) to target two specific single-nucleotide missense mutations on KRAS codon-12 located in the seed region adjacent to a protospacer adjacent motif (PAM). Lentiviral transduction of Cas9 and the sgRNAs into cancer cells with respective KRAS mutations resulted in high frequency of indels in the seed region. Indel-associated disruption of the mutant KRAS alleles correlated with reduced viability of the cancer cells. The results indicate that CRISPR-Cas9-mediated genome editing can potentially be used for the treatment of cancer patients, specifically those with oncogenic KRAS mutations.
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http://dx.doi.org/10.1038/s41598-018-30205-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6082849PMC
August 2018

Clonal Expansion of Macrolide-Resistant Sequence Type 3 Mycoplasma pneumoniae, South Korea.

Emerg Infect Dis 2018 08;24(8):1465-1471

To investigate the genetic background for the emergence of macrolide resistance, we characterized the genetic features of Mycoplasma pneumoniae using multilocus sequence typing. Of the 146 M. pneumoniae strains collected during the 5 consecutive outbreaks of M. pneumoniae pneumonia during 2000-2016 in South Korea, macrolide resistance increased from 0% in the first outbreak to 84.4% in the fifth. Among the 8 sequence types (STs) identified, ST3 (74.7%) was the most prevalent, followed by ST14 (15.1%). Macrolide-susceptible strains comprised 8 different STs, and all macrolide-resistant strains were ST3 (98.3%) except 1 with ST14. The proportion of macrolide-resistant strains in ST3 remained 2.2% (1/46) until the 2006-2007 outbreak and then markedly increased to 82.6% (19/23) during the 2010-2012 outbreak and 95.0% (38/40) during the 2014-2016 outbreak. The findings demonstrated that clonal expansion of ST3 M. pneumoniae was associated with the increase in macrolide resistance in South Korea.
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http://dx.doi.org/10.3201/eid2408.180081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056092PMC
August 2018

Statistics on noise covariance matrix for covariance fitting-based compressive sensing direction-of-arrival estimation algorithm: For use with optimization via regularization.

J Acoust Soc Am 2018 Jun;143(6):3883

Department of Information and Communication Engineering, Sejong University, Seoul 05006, Republic of Korea.

A covariance fitting algorithm for the estimation of direction-of-arrivals of multiple incident signals is addressed in this paper. The scheme takes advantage of the fact that the incident signals are spatially sparse. A previous study has presented the regularization parameters of the covariance fitting for a very large number of snapshots. In this paper, a strategy on how to determine the regularization constant of the covariance fitting for a general number of snapshots is presented. The strategy essentially exploits the norm of the noise covariance matrix. The proposed algorithm has been validated via numerical simulations.
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http://dx.doi.org/10.1121/1.5042354DOI Listing
June 2018

Mental health stigma: another enemy for defeat chronic pain.

Authors:
Joon Ho Lee

Korean J Pain 2018 Apr 2;31(2):71-72. Epub 2018 Apr 2.

Department of Anesthesiology and Pain Medicine, Soonchunhayng University Bucheon Hospital, Bucheon, Korea.

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http://dx.doi.org/10.3344/kjp.2018.31.2.71DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904349PMC
April 2018