Publications by authors named "Jong-Bae Kim"

78 Publications

Antidiabetic effects of betulinic acid mediated by the activation of the AMP-activated protein kinase pathway.

PLoS One 2021 5;16(4):e0249109. Epub 2021 Apr 5.

Department of Obstetrics and Gynecology, Ajou University Hospital, Ajou University School of Medicine, Suwon, South Korea.

Betulinic acid (BA) is a naturally arising pentacyclic triterpenoid that has anti-malarial, anti-retroviral, anti-inflammatory, and anti-cancer biological effects. More recently, it has been reported to possess anti-obesity activity mediated by the activation of AMP-activated protein kinase (AMPK). We further investigated antidiabetic activity of BA in mouse tissues at the cellular and systemic levels. We found that BA stimulated AMPK in a similar fashion to the known AMPK activators, such as 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside and metformin. Notably, the level of glucose uptake by BA was not altered by wortmannin, suggesting that this activation did not depend on phosphoinositide 3-kinase. Furthermore, BA diminished blood glucose levels in alloxane-treated ICR mice and in untreated mice during the glucose tolerance test. BA also stimulated mRNA expression of glucose transporter 4, which could partly explain increased glucose uptake. BA also increased AS160 phosphorylation by insulin-independent mechanisms in the extensor digitorum longus muscle. These results indicate that BA may serve as a promising therapeutic agent for diabetes by activating AMPK, like metformin. Notably, BA also enhanced mouse endurance capacity, indicating that it also affects metabolic regulation in addition to its antidiabetic activity.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249109PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021171PMC
April 2021

Evodiamine Inhibits Growth and -Induced Inflammation.

Int J Mol Sci 2021 Mar 25;22(7). Epub 2021 Mar 25.

Department of Clinical Laboratory Science, Semyung University, Jecheon 27136, Korea.

() classified as a class I carcinogen by the World Health Organization (WHO) plays an important role in the progression of chronic gastritis and the development of gastric cancer. A major bioactive component of , evodiamine, has been known for its anti-bacterial effect and anti-cancer effects. However, the inhibitory effect of evodiamine against is not yet known and the inhibitory mechanisms of evodiamine against gastric cancer cells are yet to be elucidated concretely. In this study, therefore, anti-bacterial effect of evodiamine on growth and its inhibitory mechanisms as well as anti-inflammatory effects and its mechanisms of evodiamine on -induced inflammation were investigated in vitr. Results of this study showed the growth of the reference strains and clinical isolates were inhibited by evodiamine. It was considered one of the inhibitory mechanisms that evodiamine downregulated both gene expressions of replication and transcription machineries of . Treatment of evodiamine also induced downregulation of urease and diminished translocation of cytotoxin-associated antigen A (CagA) and vacuolating cytotoxin A (VacA) proteins into gastric adenocarcinoma (AGS) cells. This may be resulted from the reduction of CagA and VacA expressions as well as the type IV secretion system (T4SS) components and secretion system subunit protein A (SecA) protein which are involved in translocation of CagA and VacA into host cells, respectively. In particular, evodiamine inhibited the activation of signaling proteins such as the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) and the mitogen-activated protein kinase (MAPK) pathway induced by infection. It consequently might contribute to reduction of interleukin (IL)-8 production in AGS cells. Collectively, these results suggest anti-bacterial and anti-inflammatory effects of evodiamine against .
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http://dx.doi.org/10.3390/ijms22073385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036659PMC
March 2021

Effects of occupational balance on subjective health, quality of life, and health-related variables in community-dwelling older adults: A structural equation modeling approach.

PLoS One 2021 11;16(2):e0246887. Epub 2021 Feb 11.

Department of Occupation Therapy, Yonsei University, Wonju, Republic of Korea.

Occupational balance is an important variable associated with health and quality of life. This study aimed to investigate the influence of occupational balance on health, quality of life, and other health-related variables using structural equation modeling. We analyzed data from 208 adults over 55 years old. Mean age of the participants was 70.21 years (SD 7.22). The research model for analysis was based on the results of previous studies addressing occupational balance and related variables such as stress, leisure satisfaction, life satisfaction, subjective health, quality of life, and participation. General fit indices of the final model were acceptable (x2/df = 1.708, p < .001, RMSEA = .058, TLI = .923, CFI = .929, and SRMR = .067). Although the size of effect was small to medium (.157-.249), occupational balance was identified as an independent variable directly or indirectly affecting subjective health, quality of life, and health-related variables in the final model. Our results showed that it is possible to improve subjective health and quality of life by promoting better occupational balance. Further studies developing an intervention program based on occupational balance are required to confirm the feasibility of the intervention and its effect on older adults' health and quality of life in real-life circumstances.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246887PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877622PMC
February 2021

The Development of Leisure Participation Assessment Tool for the Elderly.

Occup Ther Int 2020 4;2020:9395629. Epub 2020 Dec 4.

Dept. of Occupational Therapy, College of Health Science, Yonsei University, 26493, 1, Yeonsedae-gil, Heungeop-myeon, Wonju-si, Gangwon-do, Republic of Korea.

Objectives: This study is aimed at developing multidimensional leisure participation assessment tool for the elderly to achieve quantitative and qualitative assessment of leisure participation and leisure exploration.

Methods: This study collected preliminary items through literature review, statistical office data, and survey of the elderly's leisure activities and considered the list of leisure activities as assessment items by conducting a Delphi survey. Reliability was verified through internal consistency and test-retest reliability. The assessment tool was finally confirmed using content validity and discriminant validity.

Results: A total of 81 leisure items classified into 8 categories and 22 subcategories were obtained through data collection and Delphi survey. Cronbach's value was 0.939, and Intraclass Correlation Coefficient was 0.941. A content validity test was confirmed by validating that I-CVI was 0.78 or more and the S-CVI was 0.95. According to the result of discriminant validity, there was a difference in the number of participating leisure activities and leisure activities with participation intention by age.

Conclusion: The leisure participation assessment tool for the elderly developed in this study can obtain information on the overall view of the leisure of the elderly by measuring leisure exploration, leisure participation, and interference factor affecting leisure participation.
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http://dx.doi.org/10.1155/2020/9395629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787791PMC
March 2021

Sub-100-nm Nearly Monodisperse n-Paraffin/PMMA Phase Change Nanobeads.

Nanomaterials (Basel) 2021 Jan 14;11(1). Epub 2021 Jan 14.

School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea.

In this study, we demonstrate the colloidal synthesis of nearly monodisperse, sub-100-nm phase change material (PCM) nanobeads with an organic n-paraffin core and poly(methylmethacrylate) (PMMA) shell. PCM nanobeads are synthesized via emulsion polymerization using ammonium persulfate as an initiator and sodium dodecylbenzenesulfonate as a surfactant. The highly uniform n-paraffin/PMMA PCM nanobeads are sub-100 nm in size and exhibit superior colloidal stability. Furthermore, the n-paraffin/PMMA PCM nanobeads exhibit reversible phase transition behaviors during the n-paraffin melting and solidification processes. During the solidification process, multiple peaks with relatively reduced phase change temperatures are observed, which are related to the phase transition of n-paraffin in the confined structure of the PMMA nanobeads. The phase change temperatures are further tailored by changing the carbon length of n-paraffin while maintaining the size uniformity of the PCM nanobeads. Sub-100-nm-sized and nearly monodisperse PCM nanobeads can be potentially utilized in thermal energy storage and drug delivery because of their high colloidal stability and solution processability.
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http://dx.doi.org/10.3390/nano11010204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830838PMC
January 2021

Development and application of PyeongChang 2018 Winter Olympic and Paralympic Games Accessibility Manual.

Disabil Rehabil Assist Technol 2020 May 18:1-7. Epub 2020 May 18.

Department of Occupational Therapy School of Health Science, College of Health Science, Yonsei University, Wonju, South Korea.

In this study, the process of development, application, and evaluation of the PyeongChang Organising Committee for the 2018 Olympic and Paralympic Winter Games(POCOG) Accessibility Manual was introduced and the basic data for accessing venues and ways to improve accessible sporting event management were suggested. Analysing and comparing the IPC Guide and Korean accessibility laws with eight advisory meetings were held to develop "POCOG Accessibility Manual". To verify the Manual standards, accessibility assessment of the Paralympic Test-event, Olympic and Paralympic Games was conducted. POCOG Accessibility Manual was developed based on the best practice of accessibility standards and feedback from various experts. Throughout the three-step verifications, many findings have resulted in the final recommendations for the improvements in the operation of the Olympic and Paralympic Games accessibility. The study provided fundamental data to develop an inclusive game operation manual for future international sports competitions, and it could ultimately serve as a cornerstone for integration and inclusion through sports.Implications for RehabilitationEnsuring accessibility can secure equal opportunities to participate and enjoy the events for everyone in mega-sporting events including persons with disabilities.However, the current Korean construction law does not adequately address the accessibility requirements for the Paralympic Games. Therefore, the study of the development of the 2018 Pyeongchang Accessibility Manual based on IPC guidelines was important.In this study, the 2018 Pyeongchang Accessibility Manual was applied and evaluated according to the planning stage of the 2017 Paralympic test-events, PyeongChang 2018 Winter Olympic and paralympic Games.The result derived from our study will provide a strategy to ensure the accessibility for persons with disabilities to countries hosting future sports events.
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http://dx.doi.org/10.1080/17483107.2020.1738564DOI Listing
May 2020

ICT-Based Health Care Services for Individuals with Spinal Cord Injuries: A Feasibility Study.

Sensors (Basel) 2020 Apr 28;20(9). Epub 2020 Apr 28.

Department of Occupational Therapy, College of Health Science, Yonsei University, 26493 Wonju, Korea.

In the Republic of Korea, 90.5% of those living with spinal cord injury (SCI) are faced with medical complications that require chronic care. Some of the more common ones include urinary tract infections, pressure sores, and pain symptomatology. These and other morbidities have been recognized to deteriorate the individual's health, eventually restricting their community participation. Telerehabilitation, using information and communication technology, has propelled a modern-day movement in providing comprehensive medical services to patients who have difficulty in mobilizing themselves to medical care facilities. This study aims to verify the effectiveness of health care and management in the SCI population by providing ICT-based health care services. We visited eight individuals living with chronic SCI in the community, and provided ICT-based health management services. After using respiratory and urinary care devices with the provision of home visit occupational therapy, data acquisition was achieved and subsequently entered into a smart device. The entered information was readily accessible to the necessary clinicians and researchers. The clients were notified if there were any concerning results from the acquired data. Subsequently, they were advised to follow up with their providers for any immediate medical care requirements. Digital hand-bike ergometers and specialized seating system cushions are currently in development. The ICT-based health care management service for individuals with SCI resulted in a favorable expected level of outcome. Based on the results of this study, we have proposed and are now in preparation for a randomized clinical trial.
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http://dx.doi.org/10.3390/s20092491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249337PMC
April 2020

The Effects of Sulglycotide on the Adhesion and the Inflammation of .

Int J Environ Res Public Health 2020 04 23;17(8). Epub 2020 Apr 23.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Korea.

() is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat various gastric diseases. We investigated the inhibitory effects of sulglycotide on adhesion and inflammation after infection in human gastric adenocarcinoma cells (AGS cells). reference strain 60190 (ATCC 49503) was cultured on Brucella agar supplemented with 10% bovine serum. Sulgylcotide-mediated growth inhibition of was evaluated using the broth dilution method. Inhibition of adhesion to AGS cells by sulglycotide was assessed using a urease assay. Effects of sulglycotide on the translocation of virulence factors was measured using western blot to detect cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) proteins. Inhibition of IL-8 secretion was measured using enzyme-linked immunosorbent assay (ELISA) to determine the effects of sulglycotide on inflammation. Sulglycotide did not inhibit the growth of , however, after six and 12 hours of infection on AGS cells, adhesion was significantly inhibited by approximately 60% by various concentrations of sulglycotide. Sulglycotide decreased virulence factor (CagA and VacA) translocation to AGS cells and inhibited IL-8 secretion. Sulglycotide inhibited adhesion and inflammation after infection of AGS cells in vitro. These results support the use of sulglycotide to treat infections.
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http://dx.doi.org/10.3390/ijerph17082918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215434PMC
April 2020

Inhibitory Effects of β-Caryophyllene on Infection In Vitro and In Vivo.

Int J Mol Sci 2020 Feb 3;21(3). Epub 2020 Feb 3.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Korea.

The human specific bacterial pathogen () is associated with severe gastric diseases, including gastric cancer. Recently, the increasing resistance makes the usage of antibiotics less effectively. Therefore, development of a new antimicrobial agent is required to control infection. In the current study, the inhibitory effect of β-caryophyllene on growth, as well as the antibacterial therapeutic effect, has been demonstrated. β-caryophyllene inhibited growth via the downregulation of E, N, B, and A and also downregulated virulence factors such as CagA, VacA, and SecA proteins. β-caryophyllene inhibited expression of several T4SS components, so that CagA translocation into -infected AGS gastric cancer cells was decreased by β-caryophyllene treatment. β-caryophyllene also inhibited VacA entry through the downregulation of T5SS. After β-caryophyllene administration on Mongolian gerbils, the immunohistochemistry (IHC) and Hematoxylin&Eosin stains showed therapeutic effects in the treated groups. Hematological data, which was consistent with histological data, support the therapeutic effect of β-caryophyllene administration. Such a positive effect of β-caryophyllene on infection potently substantiates the natural compound as being capable of being used as a new antimicrobial agent or functional health food to help patients who are suffering from gastroduodenal diseases due to infection.
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http://dx.doi.org/10.3390/ijms21031008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037973PMC
February 2020

Associating Psychotic Symptoms with Altered Brain Anatomy in Psychotic Disorders Using Multidimensional Item Response Theory Models.

Cereb Cortex 2020 05;30(5):2939-2947

Departments of Medicine and Public Health Sciences, University of Chicago, Chicago, IL 60637, USA.

Reduced cortical thickness has been demonstrated in psychotic disorders, but its relationship to clinical symptoms has not been established. We aimed to identify the regions throughout neocortex where clinical psychosis manifestations correlate with cortical thickness. Rather than perform a traditional correlation analysis using total scores on psychiatric rating scales, we applied multidimensional item response theory to identify a profile of psychotic symptoms that was related to a region where cortical thickness was reduced. This analysis was performed using a large population of probands with psychotic disorders (N = 865), their family members (N = 678) and healthy volunteers (N = 347), from the 5-site Bipolar-Schizophrenia Network for Intermediate Phenotypes. Regional cortical thickness from structural magnetic resonance scans was measured using FreeSurfer; individual symptoms were rated using the Positive and Negative Syndrome Scale, Montgomery-Asberg Depression Rating Scale, and Young Mania Rating Scale. A cluster of cortical regions whose thickness was inversely related to severity of psychosis symptoms was identified. The regions turned out to be located contiguously in a large region of heteromodal association cortex including temporal, parietal and frontal lobe regions, suggesting a cluster of contiguous neocortical regions important to psychosis expression. When we tested the relationship between reduced cortical surface area and high psychotic symptoms we found no linked regions describing a related cortical set.
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http://dx.doi.org/10.1093/cercor/bhz285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197072PMC
May 2020

Stakeholder perspectives on research and development priorities for mobility assistive-technology: a literature review.

Disabil Rehabil Assist Technol 2019 Sep 19:1-15. Epub 2019 Sep 19.

VA Pittsburgh Healthcare System, HERL , Pittsburgh , PA , USA.

Over one billion people with disabilities (PWDs) and older adults with mobility impairment are currently in need of assistive technology devices (ATDs) and only 10% of those population have ordinarily access to them. The need for advancement in mobility-assistive technology is growing to address the gap in ATDs provision globally. The purpose of this review is to identify potential future areas of development and research in mobility-assistive technology. Publications were identified using scientific and medical electronic databases. Also, a limited grey literature search was conducted to muster a variety of sources. A combination of keyword search terms was used, corresponding to the medical subject heading (MeSH) terms. A total of 392 articles were identified, of which 75 were selected for detailed review. Twenty-eight articles were identified that met the review's inclusion criteria. Future areas of research for mobility-assistive technology were identified by grouping the publications into four main categories. The findings of this review identified several areas of research and development in ATDs in general and mobility-assistive technology, in particular, with special attention to the importance of engaging users and stakeholders in the process of research and design. It is apparent that users' needs and priorities vary between regions within countries. The majority of studies were noted to mainly identify consumers' perspectives on a national basis. The authors, therefore, suggest that further research should be conducted on a global level to determine the knowledge and perspectives concerning future research and development needs and priorities in mobility-assistive technologies. Implication for Rehabilitation Despite the benefits derived from the use of ATDs, only 10% of people with disabilities have access to them. Increasing access, quality and affordable ATDs in all countries is global demand. Identifying mobility consumers' needs and priorities would help in enhancing their quality of life by translating research into new technologies that meet their environment and culture needs. Users' involvement in research and design process is a crucial approach to re-shape the future research agenda.
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http://dx.doi.org/10.1080/17483107.2019.1650300DOI Listing
September 2019

Stakeholder perspectives on research and development priorities for mobility assistive-technology: a literature review.

Disabil Rehabil Assist Technol 2019 Sep 19:1-15. Epub 2019 Sep 19.

VA Pittsburgh Healthcare System, HERL , Pittsburgh , PA , USA.

Over one billion people with disabilities (PWDs) and older adults with mobility impairment are currently in need of assistive technology devices (ATDs) and only 10% of those population have ordinarily access to them. The need for advancement in mobility-assistive technology is growing to address the gap in ATDs provision globally. The purpose of this review is to identify potential future areas of development and research in mobility-assistive technology. Publications were identified using scientific and medical electronic databases. Also, a limited grey literature search was conducted to muster a variety of sources. A combination of keyword search terms was used, corresponding to the medical subject heading (MeSH) terms. A total of 392 articles were identified, of which 75 were selected for detailed review. Twenty-eight articles were identified that met the review's inclusion criteria. Future areas of research for mobility-assistive technology were identified by grouping the publications into four main categories. The findings of this review identified several areas of research and development in ATDs in general and mobility-assistive technology, in particular, with special attention to the importance of engaging users and stakeholders in the process of research and design. It is apparent that users' needs and priorities vary between regions within countries. The majority of studies were noted to mainly identify consumers' perspectives on a national basis. The authors, therefore, suggest that further research should be conducted on a global level to determine the knowledge and perspectives concerning future research and development needs and priorities in mobility-assistive technologies. Implication for Rehabilitation Despite the benefits derived from the use of ATDs, only 10% of people with disabilities have access to them. Increasing access, quality and affordable ATDs in all countries is global demand. Identifying mobility consumers' needs and priorities would help in enhancing their quality of life by translating research into new technologies that meet their environment and culture needs. Users' involvement in research and design process is a crucial approach to re-shape the future research agenda.
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http://dx.doi.org/10.1080/17483107.2019.1650300DOI Listing
September 2019

Inhibitory Effects of Menadione on Growth and -Induced Inflammation via NF-κB Inhibition.

Int J Mol Sci 2019 Mar 7;20(5). Epub 2019 Mar 7.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju 26493, Korea.

is classified as a group I carcinogen by WHO because of its involvement in gastric cancer development. Several reports have suggested anti-bacterial effects of menadione, although the effect of menadione on major virulence factors of and -induced inflammation is yet to be elucidated. In this study, therefore, we demonstrated that menadione has anti- and anti-inflammatory effects. Menadione inhibited growth of reference strains and clinical isolates. Menadione reduced expression of A in , and translocation of VacA protein into AGS (gastric adenocarcinoma cell) was also decreased by menadione treatment. This result was concordant with decreased apoptosis in AGS cells infected with . Moreover, cytotoxin-associated protein A (CagA) translocation into -infected AGS cells was also decreased by menadione. Menadione inhibited expression of several type IV secretion system (T4SS) components, including B2, B7, B8, and B10, that are responsible for translocation of CagA into host cells. In particular, menadione inhibited nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation and thereby reduced expression of the proinflammatory cytokines such as IL-1β, IL-6, IL-8, and TNF-α in AGS as well as in THP-1 (monocytic leukemia cell) cell lines. Collectively, these results suggest the anti-bacterial and anti-inflammatory effects of menadione against .
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http://dx.doi.org/10.3390/ijms20051169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429389PMC
March 2019

Influence of the Sintering Temperature of Al-Doped Higher Manganese Silicide for Improved Thermoelectric Properties.

J Nanosci Nanotechnol 2019 Mar;19(3):1699-1703

Department of Materials Science and Engineering, Inha University, Incheon 22212, Republic of Korea.

Higher manganese silicide is generally used in thermoelectric devices between 700 K and 900 K. MnSiAl samples were fabricated by two continuous solid-state reactions followed by hot pressing because the electrical conductivity of all the samples is strongly dependent on Al doping, showing superior thermoelectric performance to the as-synthesized higher manganese silicide. The solid-state-reaction was performed at 1173 K for 6 hours. The effects of the sintering temperature were examined by sintering at three different temperatures: 1273 K, 1323 K and 1373 K. For the surface, microstructural, and electrical properties, scanning electron microscopy, X-ray diffraction, and a series of electric conductivity, Seebeck coefficient, and thermal conductivity analyses were conducted, respectively. As a result, the optimal process temperature for Al-doped higher manganese silicide using a hot-press technique was determined.
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http://dx.doi.org/10.1166/jnn.2019.16188DOI Listing
March 2019

Intermetallic Growth Mechanism and Mechanical Properties of Post-Annealed SAC305 Solder Joints on Cu-Based Electrode Interfaces.

J Nanosci Nanotechnol 2019 Mar;19(3):1645-1648

Department of Materials Science and Engineering, Inha University, Incheon 22212, Republic of Korea.

Intrinsic Cu- and Ni-added Cu electrodes were prepared to study Sn-3Ag-0.5Cu lead-free solder joints. Our work focused on three categories: (1) formation and role of intermetallic compounds, (2) structural and compositional change of intermetallic compounds due to thermal aging effects, and (3) mechanical bonding strength of solder joints. A series of SEM, EDX, and bonding test analyses were performed on two electrode types to study joint morphologies, the types of intermetallic compounds formed, and bonding strengths, respectively. As a result, after heat treatments at 150 °C for 10 h, 100 h, and 300 h, CuSn and (Ni, Cu)₃Sn₄ were obtained at the interfaces of the intrinsic Cu electrode and the Ni-added Cu electrode, respectively. In the Ni-added Cu electrode samples, the growth rate of the intermetallic compounds was reduced, but the mechanical bonding strength had a higher value compared to that of the intrinsic Cu electrode. The bonding characteristics under different heat treatment conditions are also discussed.
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http://dx.doi.org/10.1166/jnn.2019.16187DOI Listing
March 2019

Thermoelectric Properties of Cu-Doped Bi₂TeSe Fabricated by Hot Pressing.

J Nanosci Nanotechnol 2019 Mar;19(3):1533-1537

Department of Material Science and Engineering, INHA University, Incheon 22212, Korea.

Owing to the energy and environmental issues, energy recovery technologies attract an increasing interest. Thermoelectric power generation is a recycling technology, which directly converts heat energy into electric energy by reusing waste heat. In this study, -type Bi₂TeSe thermoelectric materials doped with Cu were fabricated by hot pressing. The Bi-Te system has excellent thermoelectric properties in the middle- and low-temperature ranges; when a certain amount of Cu dopant is added, the thermoelectric properties are improved. The thermoelectric properties of the samples doped with Cu were compared with those of the intrinsic Bi-Te-based sample without Cu doping. In addition, the effects of the Cu concentration on the thermoelectric-material structures were investigated.
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http://dx.doi.org/10.1166/jnn.2019.16199DOI Listing
March 2019

Anti-inflammatory effects of Kaempferol on Helicobacter pylori-induced inflammation.

Biosci Biotechnol Biochem 2019 Jan 5;83(1):166-173. Epub 2018 Oct 5.

a Department of Biomedical Laboratory Science, College of Health Sciences , Yonsei University , Wonju , Republic of Korea.

Inflammation induced by Helicobacter pylori infection related to gastric carcinogenesis. In this study, we have investigated the anti-inflammatory effect and its mechanism of kaempferol in the inflammatory response caused by H. pylori infection in vitro. We found that kaempferol reduced the expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-8) and production of IL-8 in AGS cells. In addition, kaempferol suppressed translocation of cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA) of H. pylori to AGS cells. It was due to decreased transcription of type IV secretion system (T4SS) components involved in CagA injection and secretion system subunit protein A (SecA) of type V secretion system (T5SS) involved in VacA secretion by kaempferol. In conclusion, kaempferol shows the anti-inflammatory effect by suppressing the translocation of CagA and VacA proteins and leading to the down-regulation of pro-inflammatory cytokines. Abbreviations: CagA: cytotoxin-associated gene A; VacA: vacuolating cytotoxin A; T4SS: type IV secretion systems; SecA: secretion system subunit protein A; T5SS: type V secretion system.
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http://dx.doi.org/10.1080/09168451.2018.1528140DOI Listing
January 2019

Korean mistletoe lectin enhances natural killer cell cytotoxicity via upregulation of perforin expression.

Asian Pac J Allergy Immunol 2018 Sep;36(3):175-183

School of Life Science, Handong Global University, Pohang, Gyeongbuk 79170, Republic of Korea.

Background: Natural killer (NK) cells are crucial components of the innate immune system, providing the first line of defense against pathogens. In a previous study, we demonstrated prophylactic activity of water extract of Korean mistletoe (Viscum album coloratum) on tumor metastasis. However, the leading compound from water extract of Korean mistletoe was not clearly addressed.

Objective: The purpose of this research was mainly focused on addressing the effect of Korean mistletoe lectin (KMLC) on NK cell cytotoxicity, and the ability of cytokine secretion as well as its signal transduction, mitogen-activated protein kinase (MAPK) pathway.

Methods: KMLC was used to test NK cell-mediated cytotoxicity in vitro and in vivo. Non-isotope cytotoxicity assay (bis-N,N,N',N'-tetraacetic acid (BATDA) release assay) was performed to test the cytotoxicity of NK cells against target tumor cells. Receptor expression was checked by flow cytometry analysis and MAPK signal molecules were analyzed by immunoblotting.

Results And Conclusions: KMLC at 200 ng/mL increased the cytotoxicity of NK92 cells by 35% compared with untreated cells. KMLC-treated (at 100 ng/mL) mice splenocytes showed a 20% increase in cytotoxic activity. Also, the B chain, one of the subchains of KMLC, increases perforin expression. We demonstrated that the signal transduction controlling NK cell cytotoxicity was mediated by upregulation of the NKG2D receptor and expression of a cytotoxic effector molecule. These results suggested that KMLC possessed immunological activity, mediated by NK cell activation.
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http://dx.doi.org/10.12932/AP-030417-0067DOI Listing
September 2018

Effects of Heat Treatment on Interfacial Behavior and Bonding Strength of Surface Activated Bonding Ti-Al Laminate.

J Nanosci Nanotechnol 2018 Mar;18(3):1847-1850

Department of Materials Engineering, Inha University, Incheon 22212, Republic of Korea.

The surface activated bonding (SAB) method generally has the advantage of high bonding strength, low contact resistance, and high microstructural stability at room temperature. In this study, Ti-Al laminates were produced by surface activated bonding with aluminum and titanium foils. Heat treatment was conducted at the temperature range from 200 to 550 °C in vacuum. The bonding strength Ti-Al laminates was measured by a peel test, and the interfacial characteristics were investigated microstructural observation. The results showed that the bonding strength was the highest with heat treatment at 400 °C, microstructure observation revealed that the bonding strength of the Ti-Al laminate was influenced by the interfacial characteristics.
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http://dx.doi.org/10.1166/jnn.2018.14992DOI Listing
March 2018

Regulatory Effects of Black Rice Extract on Helicobacter pylori Infection-Induced Apoptosis.

Mol Nutr Food Res 2018 02 14;62(3). Epub 2017 Dec 14.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Republic of Korea.

Scope: Black rice extract (BRE) contains cyanidin 3-O-glucoside (C3G), an anthocyanin, as the major component. In this study, we found that BRE inhibits the mRNA and protein expression of genes encoding cytotoxin-associated protein A (cagA) and vacuolating protein A (vacA) in Helicobacter pylori 60190 strain.

Methods And Results: We performed RT-PCR and western blotting to show that BRE inhibits the mRNA and protein expression of SecA. Because SecA is involved in VacA export in bacteria, our result suggests a positive correlation between BRE-induced inhibition of secA expression and VacA secretion. Further, we perform MTT assay and flow cytometry to show that BRE decreases the apoptosis of H. pylori-infected KATO III cells. Finally, we perform western blotting to show that the cell-protective effect of BRE is associated with decreased levels of active proapoptotic proteins caspases and PARP and increased levels of antiapoptotic proteins survivin and XIAP in H. pylori-infected cells.

Conclusion: Thus, our results indicate that BRE acts as a potent inhibitor of the biogenesis of H. pylori virulence proteins and decreases the apoptosis of H. pylori-infected cells. Moreover, our results suggest that BRE can be used to exert beneficial effects in patients with gastroduodenal diseases caused by H. pylori.
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http://dx.doi.org/10.1002/mnfr.201700586DOI Listing
February 2018

Antitumor Effect of KML-B-Treated Dendritic Cells via Induction of Lymphocyte Activation.

J Immunol Res 2017 29;2017:2471627. Epub 2017 May 29.

Department of Pharmacy, Sunchon National University, 255 Jungangno, Suncheon 540-950, Republic of Korea.

Lectins are carbohydrate-binding proteins with various biological activities, such as antitumor and immunomodulatory effects. Although lectins have various biological activities, they are still limited by cytotoxicity in normal cells. To overcome this problem, we used the noncytotoxic part of Korean mistletoe lectin B-chain (KML-B) to induce maturation of dendritic cells (DCs). A previous study reported that KML-B induces DC maturation by triggering TLR-4, including expression of costimulatory molecules (CD40, CD80, and CD86), MHC II, and secretion of cytokines in DCs. Additionally, matured DCs by KML-B induced T helper (Th) cell activation and differentiation toward Th1 cells. However, the interaction of KML-B-treated DCs with CD8 T cells is still poorly understood. In this study, we confirmed the ability of matured DCs by KML-B to stimulate cytotoxic T cells using OT-1 mouse-derived CD8 T cells. KML-B induced MHC I expression in DCs, stimulation of CD8 T cell activation and proliferation, and IFN- secretion. Moreover, tumor sizes were reduced by KML-B treatment during vaccination of OVA-pulsed DCs. Here, we confirmed induction of CD8 T cell activation and the antitumor effect of KML-B treatment in DCs.
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http://dx.doi.org/10.1155/2017/2471627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5467341PMC
March 2018

Carbohydrate Composition Associated with the 2-Year Incidence of Metabolic Syndrome in Korean Adults.

Clin Nutr Res 2017 Apr 24;6(2):122-129. Epub 2017 Apr 24.

Department of Foods and Nutrition, Kookmin University College of Science and Technology, Seoul 02707, Korea.

The aim of this study was to investigate the association between macronutrient composition and metabolic syndrome (MetS) incidence in Korean adults. Data were obtained from a cohort of 10,030 members aged 40 to 69 years who were enrolled from the 2 cities (Ansung and Ansan) between 2001 and 2002 to participate in the Korean Genome Epidemiology Study. Of these members, 5,565 participants, who were free of MetS and reported no diagnosis of cardiovascular disease at baseline, were included in this study. MetS was defined using the criteria of the National Cholesterol Education Program-Adult Treatment Panel III and Asia-Pacific criteria for waist circumference. MetS incidence rate were identified during a 2-year follow-up period. Baseline dietary information was obtained using a semi-quantitative food frequency questionnaire. Multivariate logistic regression analysis was used to evaluate the association between the quartiles of percentages of total calorie from macronutrients consumed and MetS incidence. In analyses, baseline information, including age, sex, body mass index, income status, educational status, smoking status, alcohol drinking status, and physical activity level was considered as confounding variables. Participants with the second quartile of the percentages of carbohydrate calorie (67%-70%) had a 23% reduced odds ratio (95% confidence interval, 0.61-0.97) for MetS incidence compared with those with the fourth quartile after adjusting for confounding variables. The findings suggest that middle aged or elderly Korean adults who consume approximately 67%-70% of calorie from carbohydrate have a reduced risk of MetS.
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http://dx.doi.org/10.7762/cnr.2017.6.2.122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5426208PMC
April 2017

Development of a Rapid Reverse Blot Hybridization Assay for Detection of Clinically Relevant Antibiotic Resistance Genes in Blood Cultures Testing Positive for Gram-Negative Bacteria.

Front Microbiol 2017 9;8:185. Epub 2017 Feb 9.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju, South Korea.

Rapid and accurate identification of the causative pathogens of bloodstream infections is crucial for the prompt initiation of appropriate antimicrobial therapy to decrease the related morbidity and mortality rates. The aim of this study was to evaluate the performance of a newly developed PCR-reverse blot hybridization assay (REBA) for the rapid detection of Gram-negative bacteria (GNB) and their extended-spectrum β-lactamase (ESBL), AmpC β-lactamase, and carbapenemase resistance genes directly from the blood culture bottles. The REBA-EAC (ESBL, AmpC β-lactamase, carbapenemase) assay was performed on 327 isolates that were confirmed to have an ESBL producer phenotype, 200 positive blood culture (PBCs) specimens, and 200 negative blood culture specimens. The concordance rate between the results of REBA-EAC assay and ESBL phenotypic test was 94.2%. The sensitivity, specificity, positive predictive value, and negative predictive value of the REBA-EAC assay for GNB identification in blood culture specimens were 100% (95% CI 0.938-1.000, < 0.001), 100% (95% CI 0.986-1.000, < 0.001), 100% (95% CI 0.938-1.000, < 0.001), and 100% (95% CI 0.986-1.000, < 0.001), respectively. All 17 EAC-producing GNB isolates from the 73 PBCs were detected by the REBA-EAC assay. The REBA-EAC assay allowed easy differentiation between EAC and non-EAC genes in all isolates. Moreover, the REBA-EAC assay was a rapid and reliable method for identifying GNB and their β-lactamase resistance genes in PBCs. Thus, this assay may provide essential information for accelerating therapeutic decisions to achieve earlier appropriate antibiotic treatment during the acute phase of bloodstream infection.
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http://dx.doi.org/10.3389/fmicb.2017.00185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299004PMC
February 2017

Korean mistletoe (Viscum album coloratum) extract regulates gene expression related to muscle atrophy and muscle hypertrophy.

BMC Complement Altern Med 2017 Jan 21;17(1):68. Epub 2017 Jan 21.

School of Life Science, Handong Global University, Pohang, 37544, Korea.

Background: Korean mistletoe (Viscum album coloratum) is a semi-parasitic plant that grows on various trees and has a diverse range of effects on biological functions, being implicated in having anti-tumor, immunostimulatory, anti-diabetic, and anti-obesity properties. Recently, we also reported that Korean mistletoe extract (KME) improves endurance exercise in mice, suggesting its beneficial roles in enhancing the capacity of skeletal muscle.

Methods: We examined the expression pattern of several genes concerned with muscle physiology in C2C12 myotubes cells to identify whether KME inhibits muscle atrophy or promotes muscle hypertrophy. We also investigated these effects of KME in denervated mice model.

Results: Interestingly, KME induced the mRNA expression of SREBP-1c, PGC-1α, and GLUT4, known positive regulators of muscle hypertrophy, in C2C12 cells. On the contrary, KME reduced the expression of Atrogin-1, which is directly involved in the induction of muscle atrophy. In animal models, KME mitigated the decrease of muscle weight in denervated mice. The expression of Atrogin-1 was also diminished in those mice. Moreover, KME enhanced the grip strength and muscle weight in long-term feeding mice.

Conclusions: Our results suggest that KME has beneficial effects on muscle atrophy and muscle hypertrophy.
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http://dx.doi.org/10.1186/s12906-017-1575-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5251312PMC
January 2017

Menadione induces G2/M arrest in gastric cancer cells by down-regulation of CDC25C and proteasome mediated degradation of CDK1 and cyclin B1.

Am J Transl Res 2016 15;8(12):5246-5255. Epub 2016 Dec 15.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju, Republic of Korea.

Menadione (vitamin K3) has been reported to induce apoptotic cell death and growth inhibition in various types of cancer cells. However, involvement of menadione in cell cycle control has not been considered in gastric cancer cells yet. In the current study, we have investigated whether menadione is involved in the cell cycle regulation and suppression of growth in gastric cancer cells. In the cell cycle analysis, we found that menadione induced G2/M cell cycle arrest in AGS cells. To elucidate the underlying mechanism, we investigated the cell cycle regulatory molecules involved in the G2/M cell cycle transition. After 24 h of menadione treatment, the protein level of CDK1, CDC25C and cyclin B1 in AGS cells was decreased in a menadione dose-dependent manner. In the time course experiment, the protein level of CDC25C decreased in 6 h, and CDK1and cyclin B1 protein levels began to decrease after 18 h of menadione treatment. We found that mRNA level of CDC25C decreased by menadione treatment in 6 h. Menadione did not have an influence on mRNA level of CDK1 and cyclin B1 though the protein levels were decreased. However, the decreased protein levels of CDK1 and cyclin B1 were recovered by inhibition of proteasome. Collectively, these results suggest that menadione inhibits growth of gastric cancer cells by reducing expression of CDC25C and promoting proteasome mediated degradation of CDK1 and cyclin B1 thereby blocking transition of the cell cycle from G2 phase to M phase.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209479PMC
December 2016

The experience of depression, anxiety, and mania among perinatal women.

Arch Womens Ment Health 2016 10 17;19(5):883-90. Epub 2016 May 17.

University of Chicago, Chicago, USA.

We assessed differential item functioning (DIF) based on computerized adaptive testing (CAT) to examine how perinatal mood disorders differ from adult psychiatric disorders. The CAT-Mental Health (CAT-MH) was administered to 1614 adult psychiatric outpatients and 419 perinatal women with IRB approval. We examined individual item-level differences using logistic regression and overall score differences by scoring the perinatal data using the original bifactor model calibration based on the psychiatric sample data and a new bifactor model calibration based on the perinatal data and computing their correlation. To examine convergent validity, we computed correlations of the CAT-MH with contemporaneously administered Edinburgh Postnatal Depression Scales (EPDS). The rate of major depression in the perinatal sample was 13 %. Rates of anxiety, mania, and suicide risk were 5, 6, and 0.4 %, respectively. One of 66 depression items, one of 69 anxiety items, and 15 of 53 mania items exhibited DIF (i.e., failure to discriminate between high and low levels of the disorder) in the perinatal sample based on the psychiatric sample calibration. Removal of these items resulted in correlations of the original and perinatal calibrations of r = 0.983 for depression, r = 0.986 for anxiety, and r = 0.932 for mania. The 91.3 % of cases were concordantly categorized as either "at-risk" or "low-risk" between the EPDS and the perinatal calibration of the CAT-MH. There was little evidence of DIF for depression and anxiety symptoms in perinatal women. This was not true for mania. Now calibrated for perinatal women, the CAT-MH can be evaluated for longitudinal symptom monitoring.
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http://dx.doi.org/10.1007/s00737-016-0632-6DOI Listing
October 2016

Piperine treatment suppresses Helicobacter pylori toxin entry in to gastric epithelium and minimizes β-catenin mediated oncogenesis and IL-8 secretion in vitro.

Am J Transl Res 2016 15;8(2):885-98. Epub 2016 Feb 15.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University Wonju, Republic of Korea.

Helicobacter pylori related gastric cancer initiation has been studied widely. The objective of our present study was to evaluate the effect of a single compound piperine on H. pylori infection and its anti-inflammatory and anti-cancer effects in vitro. Cytotoxicity was tested by Ez-cytox cell viability assay kit. Effects of piperine on H. pylori toxin gene expression and IL-8 expression in mammalian cells during infection were assessed by RT-PCR. Effects of piperine on toxin entry into host cells, E-cadherin cleavage by H. pylori, and the changes in H. pylori mediated β-catenin expression and IL-8 secretion were determined by immunoblotting. Piperine treatment restrained the entry of CagA and VacA into AGS cells. Piperine administration in H. pylori infection reduced E-cadherin cleavage in stomach epithelium. In addition, H. pylori induced β-catenin up-regulation was reduced. Piperine administration impaired IL-8 secretion in H. pylori-infected gastric epithelial cells. As we reported previously piperine restrained H. pylori motility. The possible reason behind the H. pylori inhibition mechanism of piperine could be the dwindled motility, which weakened H. pylori adhesion to gastric epithelial cells. The reduced adhesion decreased the toxin entry thereby secreting less amount of IL-8. In addition, piperine treatment suppressed H. pylori protease led to reduction of E-cadherin cleavage and β-catenin expression resulting in diminished β-catenin translocation into the nucleus thus decreasing the risk of oncogenesis. To our knowledge, this is the preliminary report of piperine mediated H. pylori infection control on gastric epithelial cells in-vitro.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4846933PMC
May 2016

The first report of NDM-5-producing uropathogenic Escherichia coli isolates in South Korea.

Diagn Microbiol Infect Dis 2016 Jun 9;85(2):198-9. Epub 2016 Feb 9.

Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju-si, Gangwon-do 26493, Republic of Korea. Electronic address:

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http://dx.doi.org/10.1016/j.diagmicrobio.2016.02.006DOI Listing
June 2016

Antimicrobial activities of ethanol and butanol fractions of white rose petal extract.

Regul Toxicol Pharmacol 2016 Apr 21;76:57-62. Epub 2016 Jan 21.

College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea. Electronic address:

White rose (Rosa hybrida) petals were extracted with ethanol (EtOH) or butanol (BuOH), and tested for their antimicrobial activities against two species of Gram-positive bacteria, six species of Gram-negative bacteria, and two species of fungi. On in vitro antimicrobial assays, Helicobacter pylori and Propionibacterium acnes were highly susceptible to white rose petal extract (WRPE)-EtOH and WRPE-BuOH, leading to minimal inhibitory concentrations of 100 and 10 μg/mL for H. pylori and 400 and 40 μg/mL for P. acnes, respectively. In in vivo experiments, C57BL/6 mice were infected with H. pylori by intragastric inoculation (1 × 10(8) CFU/mouse) 3 times, and orally treated twice a day for 14 days with WRPE-EtOH and WRPE-BuOH. On a CLO kit assay, 200 mg/kg of WRPE-EtOH fully eliminated the bacteria from the gastric mucosa, and the effect of 100 mg/kg of ethanol fraction was similar to pantoprazole (30 mg/kg), displaying 75% elimination. WRPE-BuOH was more effective, exhibiting 75% elimination at 20 mg/kg. The CLO test results were confirmed by bacterial identification. WRPE-EtOH and WRPE-BuOH inhibited the growth of various bacteria and fungi, and in particular, they effectively killed H. pylori and eliminated the bacteria from the mouse stomach. The results indicate that WRPE-EtOH and WRPE-BuOH could be good candidates for the elimination of H. pylori.
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http://dx.doi.org/10.1016/j.yrtph.2016.01.011DOI Listing
April 2016

Management of traumatic neuralgia in a patient with the extracted teeth and alveoloplasty: a case report.

J Dent Anesth Pain Med 2015 Dec 31;15(4):241-245. Epub 2015 Dec 31.

Department of Dentistry, Dong San Medical Center, Keimyung University, Daegu, Korea.

A majority of patients who sustain injuries to the peripheral sensory nerves of the face and jaws experience a slow but gradual return of sensation that is functional and tolerable, if not the same as before the injuries. However, long-term effects of such injuries are aggravating for many patients, and a few patients experience significant suffering. In some of these patients, posttraumatic symptoms become pathological and are painful. The predominant painful components are (1) numbing anesthesia dolorosa pain, (2) triggered neuralgiaform pain, (3) burning and aching causalgiaform pain, and (4) phantom pain. This is a case report of conservative management of traumatic neuralgia and neuritis as part of posttraumatic pain syndromes in geriatric patients who have undergone the teeth extraction and alveoloplasty.
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http://dx.doi.org/10.17245/jdapm.2015.15.4.241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564161PMC
December 2015