Publications by authors named "Johannes Winkler"

82Publications

Large Animal Models of Heart Failure With Reduced Ejection Fraction (HFrEF).

Front Cardiovasc Med 2019 14;6:117. Epub 2019 Aug 14.

Division of Cardiology, Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria.

View Article and Find Full Text PDF
August 2019

Targeted delivery and endosomal cellular uptake of DARPin-siRNA bioconjugates: Influence of linker stability on gene silencing.

Eur J Pharm Biopharm 2019 Aug 16;141:37-50. Epub 2019 May 16.

Department of Pharmaceutical Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria; Department of Cardiology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria. Electronic address:

View Article and Find Full Text PDF
August 2019

Covalent Fluorophore Labeling of Oligonucleotides and Generation of Other Oligonucleotide Bioconjugates.

Methods Mol Biol 2019 ;1943:61-72

Department of Pharmaceutical Chemistry, University of Vienna, Vienna, Austria.

View Article and Find Full Text PDF
July 2019

Extrahepatic Targeting of Oligonucleotides with Receptor-Binding Non-Immunoglobulin Scaffold Proteins.

Authors:
Johannes Winkler

Nucleic Acid Ther 2018 06 7;28(3):137-145. Epub 2018 May 7.

Department of Cardiology, Medical University of Vienna , Vienna, Austria .

View Article and Find Full Text PDF
June 2018

Preclinical Studies of Stem Cell Therapy for Heart Disease.

Circ Res 2018 03;122(7):1006-1020

From the Interdisciplinary Stem Cell Institute (B.A.T., W.B., J.M.H.), Department of Surgery (B.A.T.), and Department of Medicine (W.B., J.M.H.), University of Miami Miller School of Medicine, FL; Department of Cardiology, Medical University of Vienna, Austria (J.W., M.G., G.G.); Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Universidad Complutense, Madrid, Spain (F.F.-A.); and CIBERCV, ISCIII, Madrid, Spain (F.F.-A.).

View Article and Find Full Text PDF
March 2018

Critical evaluation of quantification methods for oligonucleotides formulated in lipid nanoparticles.

Int J Pharm 2018 Sep 21;548(2):793-802. Epub 2017 Dec 21.

Department of Pharmaceutics, Utrecht Institute of Pharmaceutical Sciences (UIPS), Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands. Electronic address:

View Article and Find Full Text PDF
September 2018

RNAi-Mediated Knockdown of Protein Expression.

Methods Mol Biol 2017 ;1654:351-360

Department of Pharmaceutical Chemistry, University of Vienna, Althanstraße 14, 1090, Vienna, Austria.

View Article and Find Full Text PDF
June 2018

Isolation of eudesmanes from Pluchea odorata and evaluation of their effects on cancer cell growth and tumor invasiveness in vitro.

Phytochemistry 2017 Sep 26;141:37-47. Epub 2017 May 26.

Department of Pharmacognosy, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. Electronic address:

View Article and Find Full Text PDF
September 2017

Long-term regulation of gene expression in muscle cells by systemically delivered siRNA.

J Control Release 2017 06 26;256:101-113. Epub 2017 Apr 26.

Neuroscience Institute, Lithuanian University of Health Sciences, Eiveniu str. 4, LT-50161 Kaunas, Lithuania. Electronic address:

View Article and Find Full Text PDF
June 2017

Fluorescence- and computed tomography for assessing the biodistribution of siRNA after intratracheal application in mice.

Int J Pharm 2017 Jun 14;525(2):359-366. Epub 2017 Feb 14.

Laboratory of MacroMolecular Cancer Therapeutics (MMCT), Center of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.

View Article and Find Full Text PDF
June 2017

Molecular Imaging of Angiogenesis in Cardiac Regeneration.

Curr Cardiovasc Imaging Rep 2016;9(10):27. Epub 2016 Sep 10.

Department of Cardiology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.

View Article and Find Full Text PDF
September 2016

Fragment-based solid-phase assembly of oligonucleotide conjugates with peptide and polyethylene glycol ligands.

Eur J Med Chem 2016 Oct 3;121:132-142. Epub 2016 May 3.

University of Vienna, Department of Pharmaceutical Chemistry, Althanstraße 14, 1090, Vienna, Austria. Electronic address:

View Article and Find Full Text PDF
October 2016

Triterpenoic Acids from Apple Pomace Enhance the Activity of the Endothelial Nitric Oxide Synthase (eNOS).

J Agric Food Chem 2016 Jan 30;64(1):185-94. Epub 2015 Dec 30.

Department of Pharmacognosy and ‡Department of Pharmaceutical Chemistry, University of Vienna , Althanstrasse 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
January 2016

Therapeutic oligonucleotides with polyethylene glycol modifications.

Authors:
Johannes Winkler

Future Med Chem 2015 29;7(13):1721-31. Epub 2015 Sep 29.

Department of Pharmaceutical Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
July 2016

Concise postsynthetic preparation of oligonucleotide-oligopeptide conjugates through facile disulfide bond formation.

Future Med Chem 2015 18;7(13):1657-73. Epub 2015 Sep 18.

Department of Pharmaceutical Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
July 2016

Going beyond the liver: progress and challenges of targeted delivery of siRNA therapeutics.

J Control Release 2015 Apr 4;203:1-15. Epub 2015 Feb 4.

University of Vienna, Department of Pharmaceutical Chemistry, Althanstraße 14, 1090 Vienna, Austria. Electronic address:

View Article and Find Full Text PDF
April 2015

miRNA-based therapies: strategies and delivery platforms for oligonucleotide and non-oligonucleotide agents.

Future Med Chem 2014 ;6(17):1967-84

University of Vienna, Department of Pharmaceutical Chemistry, Althanstraße 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
August 2015

Oligonucleotides conjugated with short chemically defined polyethylene glycol chains are efficient antisense agents.

Bioorg Med Chem Lett 2014 Dec 22;24(24):5758-5761. Epub 2014 Oct 22.

University of Vienna, Department of Pharmaceutical Chemistry, Althanstraße 14, 1090 Vienna, Austria. Electronic address:

View Article and Find Full Text PDF
December 2014

Chemically defined polyethylene glycol siRNA conjugates with enhanced gene silencing effect.

Bioorg Med Chem 2014 Apr 20;22(7):2320-6. Epub 2014 Feb 20.

University of Vienna, Department of Medicinal Chemistry, Althanstraße 14, 1090 Vienna, Austria. Electronic address:

View Article and Find Full Text PDF
April 2014

Novel targets for ATM-deficient malignancies.

Mol Cell Oncol 2014 13;1(1):e29905. Epub 2014 Aug 13.

Institute for Genetics; University of Cologne; Cologne, Germany.

View Article and Find Full Text PDF
June 2016

Oligonucleotide conjugates for therapeutic applications.

Authors:
Johannes Winkler

Ther Deliv 2013 Jul;4(7):791-809

Department of Medicinal Chemistry, University of Vienna, Vienna, Austria.

View Article and Find Full Text PDF
July 2013

Influence of diverse chemical modifications on the ADME characteristics and toxicology of antisense oligonucleotides.

Expert Opin Biol Ther 2013 Jun 2;13(6):875-88. Epub 2013 Mar 2.

University of Vienna, Department of Medicinal Chemistry, Althanstrasse 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
June 2013

Current status of human pluripotent stem cell based in vitro toxicity tests.

Front Biosci (Schol Ed) 2013 Jan 1;5:118-33. Epub 2013 Jan 1.

Systems toxicology unit, ECVAM, European Centre for the Validation of Alternative Methods, IHCP, JRC, European Commission, Ispra, VA, Italy.

View Article and Find Full Text PDF
January 2013

Gene expression signatures defining fundamental biological processes in pluripotent, early, and late differentiated embryonic stem cells.

Stem Cells Dev 2012 Sep 17;21(13):2471-84. Epub 2012 Apr 17.

Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Cologne, Germany.

View Article and Find Full Text PDF
September 2012

Nanomedicines based on recombinant fusion proteins for targeting therapeutic siRNA oligonucleotides.

Authors:
Johannes Winkler

Ther Deliv 2011 Jul;2(7):891-905

Department of Medicinal Chemistry, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
July 2011

Effect of chemopreventive agents on differentiation of mouse embryonic stem cells.

Front Biosci (Elite Ed) 2012 Jan 1;4:156-68. Epub 2012 Jan 1.

Center of Physiology and Pathophysiology, Institute of Neurophysiology, Robert-Koch Str. 39, 50931 Cologne, Germany.

View Article and Find Full Text PDF
January 2012

Effects of NMDA receptor modulators on a blood-brain barrier in vitro model.

Brain Res 2011 Jun 13;1394:49-61. Epub 2011 Apr 13.

Department of Anaesthesia and Critical Care, University Hospital Würzburg, Oberdürrbacherstrasse 6, 97080 Würzburg, Germany.

View Article and Find Full Text PDF
June 2011

Specific gene signatures and pathways in mesodermal cells and their derivatives derived from embryonic stem cells.

Stem Cell Rev Rep 2012 Mar;8(1):43-54

Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany.

View Article and Find Full Text PDF
March 2012

Effects of cryopreservation on the transcriptome of human embryonic stem cells after thawing and culturing.

Stem Cell Rev Rep 2011 Sep;7(3):506-17

Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931, Cologne, Germany.

View Article and Find Full Text PDF
September 2011

Off-target effects related to the phosphorothioate modification of nucleic acids.

ChemMedChem 2010 Aug;5(8):1344-52

Department of Medicinal Chemistry, University of Vienna, Austria.

View Article and Find Full Text PDF
August 2010

Isolation and functional characterization of alpha-smooth muscle actin expressing cardiomyocytes from embryonic stem cells.

Cell Physiol Biochem 2010 18;25(6):595-604. Epub 2010 May 18.

Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne (CMMC), Cologne, Germany.

View Article and Find Full Text PDF
September 2010

EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins.

Mol Cancer Ther 2009 Sep 1;8(9):2674-83. Epub 2009 Sep 1.

Institute of Pharmacology, University of Bern, 3010 Bern, Switzerland.

View Article and Find Full Text PDF
September 2009

Somitovasculin, a novel endothelial-specific transcript involved in the vasculature development.

Arterioscler Thromb Vasc Biol 2009 Nov 18;29(11):1823-9. Epub 2009 Jun 18.

University of Cologne, Center of Physiology and Pathophysiology, Institute of Neurophysiology, Cologne, Germany.

View Article and Find Full Text PDF
November 2009

Transcriptional profiling of CD31(+) cells isolated from murine embryonic stem cells.

Genes Cells 2009 Feb 15;14(2):243-60. Epub 2008 Jan 15.

Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne, Germany.

View Article and Find Full Text PDF
February 2009

Oligonucleotide-polyamine conjugates: influence of length and position of 2'-attached polyamines on duplex stability and antisense effect.

Eur J Med Chem 2009 Feb 27;44(2):670-7. Epub 2008 May 27.

Department of Medicinal Chemistry, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
February 2009

Oligonucleotide charge reversal: 2'-O-lysylaminohexyl modified oligonucleotides.

Nucleosides Nucleotides Nucleic Acids 2007 ;26(8-9):939-42

Department of Medicinal Chemistry, University of Vienna, Vienna, Austria.

View Article and Find Full Text PDF
March 2008

2'-O-Lysylaminohexyl oligonucleotides: modifications for antisense and siRNA.

ChemMedChem 2008 Jan;3(1):102-10

Department of Medicinal Chemistry, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.

View Article and Find Full Text PDF
January 2008

Cardiovascular genomics: a current overview of in vivo and in vitro studies.

Stem Cell Rev 2006 ;2(1):59-66

Center of Physiology and Pathophysiology Institute of Neurophysiology, Robert-Koch Strasse 39, Cologne, Germany.

View Article and Find Full Text PDF
February 2007

The FunGenES consortium: functional genomics in engineered embryonic stem cells.

Stem Cell Rev 2006 ;2(1):1-4

Centre of Physiology and Pathophysiology, Institute of Neurophysiology, Robert-Koch-Str. 39, 50931 Köln, Germany.

View Article and Find Full Text PDF
February 2007

Zwitterionic oligonucleotides: a study on binding properties of 2'-O-aminohexyl modifications.

Nucleosides Nucleotides Nucleic Acids 2005 ;24(8):1167-85

Department für Medizinische/Pharmazeutische Chemie, Universität Wien, Pharmaziezentrum, Althanstrasse 14, Wien 1090, Austria.

View Article and Find Full Text PDF
December 2005

An optimized embryonic stem cell model for consistent gene expression and developmental studies: a fundamental study.

Thromb Haemost 2005 Oct;94(4):719-27

Center of Physiology and Pathophysiology, Institute of Neurophysiology and Centre of Molecular Medicine, University of Cologne (CMMC), Germany.

View Article and Find Full Text PDF
October 2005

Oligonucleotides conjugated to short lysine chains.

Bioconjug Chem 2005 Jul-Aug;16(4):1038-44

Department of Medicinal Chemistry, Universität Wien, Althanstrasse 14, 1090 Wien, Austria.

View Article and Find Full Text PDF
November 2005

Embryonic stem cells for basic research and potential clinical applications in cardiology.

Biochim Biophys Acta 2005 May 9;1740(2):240-8. Epub 2004 Dec 9.

Centre of Physiology and Pathophysiology, Institute of Neurophysiology, Robert-Koch-Str. 39, 50931 Cologne, Germany.

View Article and Find Full Text PDF
May 2005

Antibodies generated by a novel DNA vaccination identify the MHC class III encoded BAT2 polypeptide.

Vaccine 2005 Mar;23(19):2540-50

Division of Immunobiology, Institute of Molecular Physiology, University of Bonn, Römerstrasse 164, 53117 Bonn, Germany.

View Article and Find Full Text PDF
March 2005

A novel concept for ligand attachment to oligonucleotides via a 2'-succinyl linker.

Nucleic Acids Res 2004 2;32(2):710-8. Epub 2004 Feb 2.

Institute for Pharmaceutical Chemistry, Universität Wien, Austria.

View Article and Find Full Text PDF
April 2004