Joana Antunes - Florida International University - Ph.D Candidate

Joana Antunes

Florida International University

Ph.D Candidate

Miami, FL

Main Specialties: Biochemical Genetics, Biotechnology

ORCID logohttps://orcid.org/0000-0003-3614-3233


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Joana Antunes - Florida International University - Ph.D Candidate

Joana Antunes

Introduction

Grant award winning Scientist, with 3+ years of hands-on experience in molecular biology & DNA sequencing. Published 6 peer-reviewed articles and 2 patents, currently serving as a reviewer for 2 journals. Organized laboratory space and executed the transition to a LIMS platform, organizing reagents supplies, inventory and ordering.
· Conceptualized an experimental strategy to identify new DNA methylation loci based on bioinformatics and high-resolution melt analysis, resulting in $50,000/year grant award
· Initiated and guided a cross-functional team, developing a novel bioinformatics analysis of 450,000 DNA methylation loci data sets, identifying new 150 potential loci
· Collaborated with an international team to validate SNaPshot analysis while managing progress of experimental work regarding identification of new markers
· Executed a cost-effective screening of pre-selected loci which resulted in the identification of 5 new biomarkers submitted to the US Patent & Trademarks Office (US Patent 10,053,740B1 from August 21, 2018)
· Assisted faculty with administrative tasks, including budgeting, grant writing and submission, and research dissemination through presentations
· Managed and organized a laboratory, monitoring use of common spaces and maintaining equipment
· Increased throughput by more than 50% through the development of an assay based on real-time PCR and DNA melt analysis

Primary Affiliation: Florida International University - Miami, FL

Specialties:


View Joana Antunes’s Resume / CV

Education

Apr 2018
Florida International University
Doctoral
Mar 2009
Universidade da Beira Interior
Masters
Research project done at Anacor Pharmaceuticals Inc
Jul 2005
Universidade da Beira Interior
Bachelors

Experience

Sep 2015
National Institute of Justice (NIJ) Graduate Student Fellowship Grant award (2015-R2-CX-0012)
Research Assistant
Feb 2014
FSF Student Scholarship Award to Register in the 66th Annual AAFS conference
Aug 2013
NIST Stipend Award covering all costs to attend the Annual IAI conference
Feb 2008 - Feb 2009
Anacor Pharmaceuticals Inc
Research Scientist I
Apr 2008
Worlds Ahead Graduate-Spring 2018

Publications

8Publications

129Reads

715Profile Views

21PubMed Central Citations

Detection and evaluation of DNA methylation markers found at SCGN and KLF14 loci to estimate human age

Alghanim, Hussain, et al. "Detection and evaluation of DNA methylation markers found at SCGN and KLF14 loci to estimate human age." Forensic Science International: Genetics 31 (2017): 81-88.

Forensic Science International : Genetics

Recent developments in the analysis of epigenetic DNA methylation patterns have demonstrated that certain genetic loci show a linear correlation with chronological age. It is the goal of this study to identify a new set of epigenetic methylation markers for the forensic estimation of human age. A total number of 27 CpG sites at three genetic loci, SCGN, DLX5 and KLF14, were examined to evaluate the correlation of their methylation status with age. These sites were evaluated using 72 blood samples and 91 saliva samples collected from volunteers with ages ranging from 5 to 73 years. DNA was bisulfite modified followed by PCR amplification and pyrosequencing to determine the level of DNA methylation at each CpG site. In this study, certain CpG sites in SCGN and KLF14 loci showed methylation levels that were correlated with chronological age, however, the tested CpG sites in DLX5 did not show a correlation with age. Using a 52-saliva sample training set, two age-predictor models were developed by means of a multivariate linear regression analysis for age prediction. The two models performed similarly with a single-locus model explaining 85% of the age variance at a mean absolute deviation of 5.8 years and a dual-locus model explaining 84% of the age variance with a mean absolute deviation of 6.2 years. In the validation set, the mean absolute deviation was measured to be 8.0 years and 7.1 years for the single- and dual-locus model, respectively. Another age predictor model was also developed using a 40-blood sample training set that accounted for 71% of the age variance. This model gave a mean absolute deviation of 6.6 years for the training set and 10.3 years for the validation set. The results indicate that specific CpGs in SCGN and KLF14 can be used as potential epigenetic markers to estimate age using saliva and blood specimens. These epigenetic markers could provide important information in cases where the determination of a suspect’s age is critical in developing investigative leads.

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November 2017
8 Reads

A collaborative exercise on DNA methylation based body fluid typing.

Electrophoresis 2016 10 19;37(21):2759-2766. Epub 2016 Sep 19.

Department of Forensic Medicine, Yonsei University College of Medicine, Seoul, South Korea.

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http://dx.doi.org/10.1002/elps.201600256DOI Listing
October 2016
18 Reads
3.030 Impact Factor

Forensic discrimination of vaginal epithelia by DNA methylation analysis through pyrosequencing.

Electrophoresis 2016 10 28;37(21):2751-2758. Epub 2016 Jun 28.

Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

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http://dx.doi.org/10.1002/elps.201600037DOI Listing
October 2016
10 Reads
3.030 Impact Factor

Developmental validation studies of epigenetic DNA methylation markers for the detection of blood, semen and saliva samples.

Forensic Sci Int Genet 2016 07 1;23:55-63. Epub 2016 Feb 1.

Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA. Electronic address:

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http://dx.doi.org/10.1016/j.fsigen.2016.01.017DOI Listing
July 2016
12 Reads
1 Citation
4.604 Impact Factor

Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing®.

Methods Mol Biol 2015 ;1315:397-409

Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami, FL, 33199, USA.

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http://dx.doi.org/10.1007/978-1-4939-2715-9_27DOI Listing
March 2016
46 Reads
1 Citation

High-resolution melt analysis of DNA methylation to discriminate semen in biological stains.

Anal Biochem 2016 Feb 22;494:40-5. Epub 2015 Oct 22.

Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA. Electronic address:

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http://dx.doi.org/10.1016/j.ab.2015.10.002DOI Listing
February 2016
9 Reads
2 Citations
2.220 Impact Factor

Evaluation of DNA methylation markers and their potential to predict human aging.

Electrophoresis 2015 Aug 14;36(15):1775-80. Epub 2015 Jul 14.

Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

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http://dx.doi.org/10.1002/elps.201500137DOI Listing
August 2015
21 Reads
8 Citations
3.030 Impact Factor

Boron-based phosphodiesterase inhibitors show novel binding of boron to PDE4 bimetal center.

FEBS Lett 2012 Sep 25;586(19):3410-4. Epub 2012 Jul 25.

Anacor Pharmaceuticals Inc., Palo Alto, CA 94303, USA.

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http://dx.doi.org/10.1016/j.febslet.2012.07.058DOI Listing
September 2012
13 Reads
9 Citations
3.170 Impact Factor

Top co-authors

George Duncan
George Duncan

School of Biological Sciences

5
Kuppareddi Balamurugan
Kuppareddi Balamurugan

School of Criminal Justice

5
Clarice S Alho
Clarice S Alho

Laboratory of Human and Molecular Genetics

3
Richard Kimura
Richard Kimura

Stanford University

1
Chen Dong
Chen Dong

The University of Texas MD Anderson Cancer Center

1
Yvonne R Freund
Yvonne R Freund

SRI International

1
Tsutomu Akama
Tsutomu Akama

University Park

1