Publications by authors named "João Victor Cabral"

10 Publications

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The healing dynamics of non-healing wounds using cryo-preserved amniotic membrane.

Int Wound J 2021 Nov 17. Epub 2021 Nov 17.

Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Prague, Czech Republic.

We evaluated the effect of the application of cryo-preserved amniotic membrane on the healing of 26 non-healing wounds (18 patients) with varying aetiologies and baseline sizes (average of 15.4 cm ), which had resisted the standard of care treatment for 6 to 456 weeks (average 88.8 weeks). Based on their average general responses to the application of cryo-preserved AM, we could differentiate three wound groups. The first healed group was characterised by complete healing (100% wound closure, maximum treatment period 38 weeks) and represented 62% of treated wounds. The wound area reduction of at least 50% was reached for all wounds in this group within the first 10 weeks of treatment. Exactly 19% of the studied wounds responded partially to the treatment (partially healed group), reaching less than 25% of closure in the first 10 weeks and 90% at maximum for extended treatment period (up to 78 weeks). The remaining 19% of treated wounds did not show any reaction to the AM application (unhealed defects). The three groups have different profiles of wound area reduction, which can be used as a guideline in predicting the healing prognosis of non-healing wounds treated with a cryo-preserved amniotic membrane.
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http://dx.doi.org/10.1111/iwj.13719DOI Listing
November 2021

Beautiful death: point of view.

Rev Assoc Med Bras (1992) 2021 04;67(4):481-484

Univervidade Federal de Pernambuco, Programa de Pós-Graduação em Ciências da Saúde - Recife (PE), Brazil.

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http://dx.doi.org/10.1590/1806-9282.20200999DOI Listing
April 2021

Triggering receptor expressed on myeloid cells-1 as pediatric sepsis biomarker.

Rev Assoc Med Bras (1992) 2021 Mar;67(3):449-453

Universidade Federal de Pernambuco, Postgraduate Program in Therapeutic Innovation - Recife (PE), Brazil.

Objective: Triggering receptor expressed on myeloid cells-1 concentration can be used as a predictive, diagnostic, and prognostic marker in patients with sepsis. The objective of this study was to determine the validity of triggering receptor expressed on myeloid cells-1 levels as a biomarker of sepsis in pediatric patients.

Methods: This was an integrative literature review. PubMed, ScienceDirect, LILACS, MEDLINE, and VHL databases were searched for papers published between 2015 and 2020, using the keywords triggering receptor expressed on myeloid cells-1, sepsis, and child.

Results: The review included ten studies, of which four used triggering receptor expressed on myeloid cells-1 as a predictive biomarker; four, as a diagnostic biomarker; and two, as a prognostic biomarker. A total of 1,409 and 1,628 patients were included in primary and review studies, respectively. There was a predominance of significant results for the validity of triggering receptor expressed on myeloid cells-1 levels in the prediction, diagnosis, and prognosis of sepsis in pediatric patients.

Conclusions: Triggering receptor expressed on myeloid cells-1 is a valid predictive, diagnostic, and prognostic biomarker of sepsis with good sensitivity and specificity in the pediatric population.
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http://dx.doi.org/10.1590/1806-9282.20200765DOI Listing
March 2021

How Can Galectin-3 as a Biomarker of Fibrosis Improve Atrial Fibrillation Diagnosis and Prognosis?

J Clin Med Res 2020 Oct 21;12(10):647-654. Epub 2020 Sep 21.

Postgraduate Program in Therapeutic Innovation, Federal University of Pernambuco (PPGIT/UFPE), Recife, PE, Brazil.

Galectin-3 (Gal-3) is a biomarker of fibrosis that has been associated with atrial remodeling. Acknowledging the presence of a biomarker in patients with atrial fibrillation (AF) can allow for a better clinical treatment. The aim of this study was to assess the association of Gal-3 with atrial fibrosis in patients with AF. This is a systematic review study. From the total number of studies analyzed, 12 demonstrated a relation between atrial fibrosis and Gal-3 in patients with AF and presented statistically significant association values. We conclude that Gal-3 is associated with atrial fibrosis in patients with AF in all types, as well as after the arrhythmia treatment by ablation.
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http://dx.doi.org/10.14740/jocmr4313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524564PMC
October 2020

Mortality due to congenital heart disease in Pernambuco from 1996 to 2016.

Rev Assoc Med Bras (1992) 2020 Jul 24;66(7):931-936. Epub 2020 Aug 24.

Programa de Pós-graduação em Ciências da Saúde, Faculdade de Ciências Médicas e Instituto de Ciências Biológicas, Universidade de Pernambuco, Recife, PE, Brasil.

BACKGROUND To determine the magnitude and temporal trends of deaths due to congenital heart disease (CHD) in Pernambuco between 1996 and 2016. METHODS This was an ecological, time-series study, involving all cases of deaths from congenital cardiovascular malformations in the state of Pernambuco, from 1996 to 2016, using data from DATASUS, SINASC and SIM. RESULTS There were 3,584 deaths from congenital cardiovascular malformations amongst individuals aged 0 to 14 years, of which 81.94% were concentrated in children aged under one year. The infant mortality rate (IMR) presented a linear growth trend of 0.4645 per year (p <0.01). The cause-of-death code Q24 (other congenital malformations of the heart) was present in 72.54% of the death records and 48.17% of the deaths occurred in infants aged between 28 and 364 days of life. The highest occurrence of deaths was identified in children with low birth weight (500 and 1,499g), male, premature, children of mothers without schooling, in deliveries at home (p <0.05). CONCLUSIONS Congenital heart disease still represents a public health problem as a cause of death, particularly in the first year of life, with IMR in a linear growth trend. Deaths from CHD were more prevalent in male children, born prematurely, with low birth weight, born to mothers with low schooling and deliveries without medical care.
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http://dx.doi.org/10.1590/1806-9282.66.7.931DOI Listing
July 2020

Ex vivo cultivated oral mucosal epithelial cell transplantation for limbal stem cell deficiency: a review.

Stem Cell Res Ther 2020 07 21;11(1):301. Epub 2020 Jul 21.

Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic.

Destruction or dysfunction of limbal epithelial stem cells (LESCs) leads to unilateral or bilateral limbal stem cell deficiency (LSCD). Fifteen years have passed since the first transplantation of ex vivo cultivated oral mucosal epithelial cells (COMET) in humans in 2004, which represents the first use of a cultured non-limbal autologous cell type to treat bilateral LSCD. This review summarizes clinical outcomes from COMET studies published from 2004 to 2019 and reviews results with emphasis on the culture methods by which grafted cell sheets were prepared.
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http://dx.doi.org/10.1186/s13287-020-01783-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7374839PMC
July 2020

Discontinuous transcription of ribosomal DNA in human cells.

PLoS One 2020 2;15(3):e0223030. Epub 2020 Mar 2.

Laboratory of Cell Biology, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic.

Numerous studies show that various genes in all kinds of organisms are transcribed discontinuously, i.e. in short bursts or pulses with periods of inactivity between them. But it remains unclear whether ribosomal DNA (rDNA), represented by multiple copies in every cell, is also expressed in such manner. In this work, we synchronized the pol I activity in the populations of tumour derived as well as normal human cells by cold block and release. Our experiments with 5-fluorouridine (FU) and BrUTP confirmed that the nucleolar transcription can be efficiently and reversibly arrested at +4°C. Then using special software for analysis of the microscopic images, we measured the intensity of transcription signal (incorporated FU) in the nucleoli at different time points after the release. We found that the ribosomal genes in the human cells are transcribed discontinuously with periods ranging from 45 min to 75 min. Our data indicate that the dynamics of rDNA transcription follows the undulating pattern, in which the bursts are alternated by periods of rare transcription events.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223030PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7051091PMC
May 2020

Burnout syndrome among medical residents: A systematic review and meta-analysis.

PLoS One 2018 12;13(11):e0206840. Epub 2018 Nov 12.

Health Science Postgraduate Program, Universidade Federal do Rio Grande do Norte, Natal, Brazil.

Background: Burnout is a psychological syndrome that is very common among medical residents. It consists of emotional exhaustion (EE), depersonalization (DP) and reduced personal accomplishment (PA).

Objective: To estimate burnout among different medical residency specialties.

Methods: A systematic review with meta-analysis was performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A search of bibliographic databases and grey literature was conducted, from inception to March 2018. The following databases were accessed: Embase, PubMed, Web of Science, Google Scholar and Scopus, and 3,575 studies were found. Methodological quality was evaluated by Agency for Healthcare Research and Quality Methodology Checklist for Cross-Sectional/Prevalence Study. In the final analysis, 26 papers were included. Their references were checked for additional studies, but none were included.

Results: 4,664 medical residents were included. High DP, EE and low PA proportions were compared. Specialties were distributed into three groups of different levels of burnout prevalence: general surgery, anesthesiology, obstetrics/gynecology and orthopedics (40.8%); internal medicine, plastic surgery and pediatrics (30.0%); and otolaryngology and neurology (15.4%). Overall burnout prevalence found for all specialties was 35.7%.

Conclusion: The prevalence of burnout syndrome was significantly higher among surgical/urgency residencies than in clinical specialties.

Prospero Registration: CRD42018090270.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0206840PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6231624PMC
April 2019

Vulvar Hemangioma: Case Report.

Rev Bras Ginecol Obstet 2018 Jun 6;40(6):369-371. Epub 2018 Jul 6.

Department of Gynecology and Obstetrics, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.

Hemangioma is a benign neoplasm that may affect the vulva, and it can cause functional or emotional disability. This article reports the case of a 52-year-old female patient with a history of a genital ulcer for the past 3 years and who had undergone various treatments with creams and ointments. The patient was biopsied and diagnosed with vulvar hemangioma and was subsequently submitted to surgical excision of the lesion. We emphasize the importance of following the steps of the differential diagnosis and proceeding with a surgical approach only if necessary.
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http://dx.doi.org/10.1055/s-0038-1657786DOI Listing
June 2018

Hyporeninemic hypoaldosteronism and diabetes mellitus: Pathophysiology assumptions, clinical aspects and implications for management.

World J Diabetes 2016 Mar;7(5):101-11

André Gustavo P Sousa, Adriana Bezerra Nunes, Department of Clinical Medicine, Federal University of Rio Grande do Norte, Natal, RN 59012-300, Brazil.

Patients with diabetes mellitus (DM) frequently develop electrolyte disorders, including hyperkalemia. The most important causal factor of chronic hyperkalemia in patients with diabetes is the syndrome of hyporeninemic hypoaldosteronism (HH), but other conditions may also contribute. Moreover, as hyperkalemia is related to the blockage of the renin-angiotensin-aldosterone system (RAAS) and HH is most common among patients with mild to moderate renal insufficiency due to diabetic nephropathy (DN), the proper evaluation and management of these patients is quite complex. Despite its obvious relationship with diabetic nephropathy, HH is also related to other microvascular complications, such as DN, particularly the autonomic type. To confirm the diagnosis, plasma aldosterone concentration and the levels of renin and cortisol are measured when the RAAS is activated. In addition, synthetic mineralocorticoid and/or diuretics are used for the treatment of this syndrome. However, few studies on the implications of HH in the treatment of patients with DM have been conducted in recent years, and therefore little, if any, progress has been made. This comprehensive review highlights the findings regarding the epidemiology, diagnosis, and management recommendations for HH in patients with DM to clarify the diagnosis of this clinical condition, which is often neglected, and to assist in the improvement of patient care.
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http://dx.doi.org/10.4239/wjd.v7.i5.101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4781902PMC
March 2016
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