Publications by authors named "Jiye Zhang"

73 Publications

The efficacy of vaccination to prevent human papilloma viruses infection at anal and oral: a systematic review and meta-analysis.

Public Health 2021 Jul 3;196:165-171. Epub 2021 Jul 3.

Medical Laboratory, Liang Ping People's Hospital of Chongqing, Liangping, 405200, Chongqing City, China. Electronic address:

Objective: The objective of this study was to review evidence on the effectiveness of vaccination in the prevention of human papilloma virus (HPV) infection at the cervix, anal, and oral.

Study Design: Systematic review and meta-analysis.

Methods: The key search limitations are as follows: "Human Papilloma Virus", "Papilloma Virus, Human" "Human Papillomavirus Virus", "HPV" and "oral", "anus", "anal", "penis", "cervical," and "vaccine". Randomized controlled studies were searched and analyzed the risk ratio by Review Manager 5.3; funnel plot was adopted for publication bias analysis.

Results: Five randomized controlled studies enrolling 13,686 participants were retrieved, analyzed, and showed that HPV vaccination can effectively block HPV infection at cervical, anal, and oral. Subgroup analysis, moreover, proved that HPV 16/18 is more effective than HPV 6/11/16/18 in preventing anal and oral infections.

Conclusion: HPV vaccine is efficacious in preventing HPV infection not only at cervical but also at anal and oral, as evidence supported by relevant studies.
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http://dx.doi.org/10.1016/j.puhe.2021.05.012DOI Listing
July 2021

Small molecule selenium-containing compounds: Recent development and therapeutic applications.

Eur J Med Chem 2021 Nov 12;223:113621. Epub 2021 Jun 12.

Department of Medicinal Chemistry, School of Pharmacy, Health Science Center, Xi'an Jiaotong University, 76 West Yanta Road, Xi'an, Shaanxi, 710061, PR China. Electronic address:

Selenium (Se) is an essential micronutrient of organism and has important function. It participates in the functions of selenoprotein in several manners. In recent years, Se has attracted much attention because of its therapeutic potential against several diseases. Many natural and synthetic organic Se-containing compounds were studied and explored for the treatment of cancer and other diseases. Studies have showed that incorporation of Se atom into small molecules significantly enhanced their bioactivities. In this paper, according to different applications and structural characteristics, the research progress and therapeutic application of Se-containing compounds are reviewed, and more than 110 Se-containing compounds were selected as representatives which showed potent activities such as anticancer, antioxidant, antifibrolytic, antiparasitic, antibacterial, antiviral, antifungal and central nervous system related effects. This review is expected to provide a basis for further study of new promising Se-containing compounds.
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http://dx.doi.org/10.1016/j.ejmech.2021.113621DOI Listing
November 2021

Transcriptional Start Site Coverage Analysis in Plasma Cell-Free DNA Reveals Disease Severity and Tissue Specificity of COVID-19 Patients.

Front Genet 2021 28;12:663098. Epub 2021 May 28.

BGI-Shenzhen, Shenzhen, China.

Symptoms of coronavirus disease 2019 (COVID-19) range from asymptomatic to severe pneumonia and death. A deep understanding of the variation of biological characteristics in severe COVID-19 patients is crucial for the detection of individuals at high risk of critical condition for the clinical management of the disease. Herein, by profiling the gene expression spectrum deduced from DNA coverage in regions surrounding transcriptional start site in plasma cell-free DNA (cfDNA) of COVID-19 patients, we deciphered the altered biological processes in the severe cases and demonstrated the feasibility of cfDNA in measuring the COVID-19 progression. The up- and downregulated genes in the plasma of severe patient were found to be closely related to the biological processes and functions affected by COVID-19 progression. More importantly, with the analysis of transcriptome data of blood cells and lung cells from control group and cases with severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection, we revealed that the upregulated genes were predominantly involved in the viral and antiviral activity in blood cells, reflecting the intense viral replication and the active reaction of immune system in the severe patients. Pathway analysis of downregulated genes in plasma DNA and lung cells also demonstrated the diminished adenosine triphosphate synthesis function in lung cells, which was evidenced to correlate with the severe COVID-19 symptoms, such as a cytokine storm and acute respiratory distress. Overall, this study revealed tissue involvement, provided insights into the mechanism of COVID-19 progression, and highlighted the utility of cfDNA as a noninvasive biomarker for disease severity inspections.
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http://dx.doi.org/10.3389/fgene.2021.663098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194351PMC
May 2021

L-Se-methylselenocysteine sensitizes lung carcinoma to chemotherapy.

Cell Prolif 2021 May 1;54(5):e13038. Epub 2021 Apr 1.

State Key Laboratory of Oncogenes and Related Genes, Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objectives: Organic Selenium (Se) compounds such as L-Se-methylselenocysteine (L-SeMC/SeMC) have been employed as a class of anti-oxidant to protect normal tissues and organs from chemotherapy-induced systemic toxicity. However, their comprehensive effects on cancer cell proliferation and tumour progression remain elusive.

Materials And Methods: CCK-8 assays were conducted to determine the viabilities of cancer cells after exposure to SeMC, chemotherapeutics or combined treatment. Intracellular reactive oxygen species (ROS) levels and lipid peroxidation levels were assessed via fluorescence staining. The efficacy of free drugs or drug-loaded hydrogel against tumour growth was evaluated in a xenograft mouse model.

Results: Among tested cancer cells and normal cells, the A549 lung adenocarcinoma cells showed higher sensitivity to SeMC exposure. In addition, combined treatments with several types of chemotherapeutics induced synergistic lethality. SeMC promoted lipid peroxidation in A549 cells and thereby increased ROS generation. Significantly, the in vivo efficacy of combination therapy was largely potentiated by hydrogel-mediate drug delivery.

Conclusions: Our study reveals the selectivity of SeMC in the inhibition of cancer cell proliferation and develops an efficient strategy for local combination therapy.
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http://dx.doi.org/10.1111/cpr.13038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088472PMC
May 2021

Dasatinib loaded nanostructured lipid carriers for effective treatment of corneal neovascularization.

Biomater Sci 2021 Apr 16;9(7):2571-2583. Epub 2021 Feb 16.

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, P. R. China.

Corneal neovascularization (CNV) is one of the most important causes of visual impairment worldwide. Dasatinib, a poorly water-soluble tyrosine kinase inhibitor with dual Src family kinase and platelet derived growth factor receptor inhibiting capability, has great potential in the treatment of CNV. In this study, dasatinib was successfully encapsulated into a nanostructured lipid carrier (Dasa-NLC) and the size was approximately 78 nm with a small polydispersity index. The NLC increased the solubility of dasatinib by more than 1220 times, sustained the drug release, reduced the ocular toxicity and facilitated its penetration into the cornea. Dasa-NLC significantly inhibited the proliferation, migration and tube formation of HUVEC cells, the three most important angiogenesis-related cellular changes of the CNV. Next, the in vivo anti-CNV effect of Dasa-NLC was evaluated using an alkaline burned mice CNV model, in which the development of the CNV and pathological changes of the cornea were significantly inhibited. The immunohistochemistry analysis indicated that Dasa-NLC could inhibit both the expression and activation of Src family kinase, a key component in the angiogenesis cascade. Therefore, Dasa-NLC showed considerable promise in the treatment of CNV.
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http://dx.doi.org/10.1039/d0bm01599gDOI Listing
April 2021

RssB-mediated σ Activation is Regulated by a Two-Tier Mechanism via Phosphorylation and Adaptor Protein - IraD.

J Mol Biol 2021 02 18;433(3):166757. Epub 2020 Dec 18.

BioBank, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 710061, China; Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, SW7 2AZ London, United Kingdom; Instrument Analysis Center of Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China. Electronic address:

Regulation of bacterial stress responding σ is a sophisticated process and mediated by multiple interacting partners. Controlled proteolysis of σ is regulated by RssB which maintains minimal level of σ during exponential growth but then elevates σ level while facing stresses. Bacteria developed different strategies to regulate activity of RssB, including phosphorylation of itself and production of anti-adaptors. However, the function of phosphorylation is controversial and the mechanism of anti-adaptors preventing RssB-σ interaction remains elusive. Here, we demonstrated the impact of phosphorylation on the activity of RssB and built the RssB-σ complex model. Importantly, we showed that the phosphorylation site - D58 is at the interface of RssB-σ complex. Hence, mutation or phosphorylation of D58 would weaken the interaction of RssB with σ. We found that the anti-adaptor protein IraD has higher affinity than σ to RssB and its binding interface on RssB overlaps with that for σ. And IraD-RssB complex is preferred over RssB-σ in solution, regardless of the phosphorylation state of RssB. Our study suggests that RssB possesses a two-tier mechanism for regulating σ. First, phosphorylation of RssB provides a moderate and reversible tempering of its activity, followed by a specific and robust inhibition via the anti-adaptor interaction.
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http://dx.doi.org/10.1016/j.jmb.2020.166757DOI Listing
February 2021

High-power vertical external-cavity surface-emitting laser emitting switchable wavelengths.

Opt Express 2020 Oct;28(22):32612-32619

In this paper we reported on the optically pumped VECSELs with switchable lasing wavelengths. The two lasing wavelengths of λ ≈ 954 nm and 1003 nm are generated at different pumping powers from the same gain chip. The thermal rollover of output power is observed twice, and the first rollover on the power curve indicates the switch of lasing wavelength. During the operation of our VECSEL, the increase of pumping power changes the temperature within the gain chip, and thus the gain spectrum is tuned to the one of two modes, which is defined by the dips on the reflectivity spectrum. The maximum output power of each wavelength exceeds 2.2 W at -5 °C. The dual-wavelength emission at λ ≈954 nm and 1003 nm is also demonstrated, and the output power of the dual-wavelength emission reached nearly 2 W.
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http://dx.doi.org/10.1364/OE.405062DOI Listing
October 2020

In vitro and in vivo effects of the combination of myricetin and miconazole nitrate incorporated to thermosensitive hydrogels, on C. albicans biofilms.

Phytomedicine 2020 Jun 12;71:153223. Epub 2020 May 12.

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, P. R. China. Electronic address:

Background: Candida albicans-related infections are common infections in clinic, among which biofilm-associated infections are most devastating and challenging to overcome. Myricetin (MY) is a plant-derived natural product with various pharmacological activities. Its anti-biofilm effect against C. albicans and its ability to increase the antifungal effect of miconazole nitrate (MN) were unclear and yet need to be explored.

Hypothesis/purpose: In this study the anti-biofilm effect of MY and its ability to increase the antifungal effect of MN were investigated in vitro and in vivo.

Study Design And Methods: MY or/and MN were incorporated into a thermosensitive hydrogel (TSH) of poloxamer. The safety of MY or/and MN loaded TSHs towards human umbilical vein endothelial cells (HUVEC) was evaluated by a MTT assay and the in vivo safety towards mice knees was confirmed by histopathological examination. The anti-biofilm effect of MY and its ability to increase the antifungal effect of MN were investigated in vitro with C. albicans ATCC 10231 by broth microdilution method, crystal violet staining and scanning electron microscopy (SEM), as well as in vivo in an established mouse model of periprosthetic joint infection (PJI) by SEM, histological analysis, microorganism culture and detection of the serum levels of interleukin-6 (IL-6). The mechanism of action of MY was analyzed by qRT-PCR assay with C. albicans SC5314.

Results: Our results showed that MY and MN incorporated into TSHs exhibited good stability and safety, excellent temperature sensitivity and controlled drug release property. MY (5-640 µg/ml) exhibited no effect on C. albicans cell viability and MY (≥80 µg/ml) showed a significantly inhibitory effect on biofilm formation. MIC (the lowest concentrations of drugs resulting in 50% decrease of C. albicans growth) and MIC (the lowest concentrations of drugs resulting in 80% decrease of C. albicans growth) of MN were respectively decreased from 2 µg/ml to 0.5 µg/ml and from 4 µg/ml to 2 µg/ml when used in combination with MY (80 µg/ml). The mouse PJI was effectively prevented by MY and MN incorporated into TSH.

Conclusions: Local application of MY and MN incorporated into TSH might be useful for clinical biofilm-associated infections.
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http://dx.doi.org/10.1016/j.phymed.2020.153223DOI Listing
June 2020

Semiconductor glass with superior flexibility and high room temperature thermoelectric performance.

Sci Adv 2020 Apr 10;6(15):eaaz8423. Epub 2020 Apr 10.

Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Most crystalline inorganic materials, except for metals and some layer materials, exhibit bad flexibility because of strong ionic or covalent bonds, while amorphous materials usually display poor electrical properties due to structural disorders. Here, we report the simultaneous realization of extraordinary room temperature flexibility and thermoelectric performance in AgTe S -based materials through amorphization. The coexistence of amorphous main phase and crystallites results in exceptional flexibility and ultralow lattice thermal conductivity. Furthermore, the flexible AgTeS glass exhibits a degenerate semiconductor behavior with a room temperature Hall mobility of ~750 cm V s at a carrier concentration of 8.6 × 10 cm, which is at least an order of magnitude higher than other amorphous materials, leading to a thermoelectric power factor also an order of magnitude higher than the best amorphous thermoelectric materials known. The in-plane prototype uni-leg thermoelectric generator made from this material demonstrates its potential for flexible thermoelectric device.
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http://dx.doi.org/10.1126/sciadv.aaz8423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148084PMC
April 2020

Precise Regulation of Carrier Concentration in Thermoelectric BiSbTe Alloys via Magnetic Doping.

ACS Appl Mater Interfaces 2020 May 23;12(18):20653-20663. Epub 2020 Apr 23.

School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, Shanghai 200444, China.

The BiTe-based alloy is the best commercial thermoelectric material around room temperature, although it is extremely difficult to further improve its thermoelectric performance. In this work, we demonstrate that magnetic doping is an effective strategy to regulate the thermoelectric performance of p-type BiSbTe. According to our experiments, it is much more difficult for ferromagnetic Fe/Co to enter the BiSbTe lattice in comparison with diamagnetic Pb, which can be understood by the "like dissolves like" rule. At the same doping content, Fe and Co provide much lower hole carriers than Pb due to their larger carrier thermal activation energies, indicating that Fe and Co as dopants are very applicable for the fine regulation of the carrier concentration. The Fe/Co-doped samples have higher Seebeck coefficients but less carrier mobilities than the Pb-doped sample since the doped magnetic atoms induce additional carrier scattering. Beyond the solid solubility limit, excess Fe/Co represents as the impurity, which can maintain a high carrier concentration due to the metal-semiconductor contact. Finally, the values of ∼1.05 and 1.15 near room temperature have been achieved for the samples with 1.71 at. % Co and 1.80 at. % Fe, respectively.
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http://dx.doi.org/10.1021/acsami.0c02408DOI Listing
May 2020

High Thermoelectric Performance of Cu-Doped PbSe-PbS System Enabled by High-Throughput Experimental Screening.

Research (Wash D C) 2020 7;2020:1736798. Epub 2020 Mar 7.

School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, Shanghai 200444, China.

Recent advances in high-throughput (HTP) computational power and machine learning have led to great achievements in exploration of new thermoelectric materials. However, experimental discovery and optimization of thermoelectric materials have long relied on the traditional Edisonian trial and error approach. Herein, we demonstrate that ultrahigh thermoelectric performance in a Cu-doped PbSe-PbS system can be realized by HTP experimental screening and precise property modulation. Combining the HTP experimental technique with transport model analysis, an optimal Se/S ratio showing high thermoelectric performance has been efficiently screened out. Subsequently, based on the screened Se/S ratio, the doping content of Cu has been subtly adjusted to reach the optimum carrier concentration. As a result, an outstanding peak zT~1.6 is achieved at 873 K for a 1.8 at% Cu-doped PbSeS sample, which is the superior value among the -type Te-free lead chalcogenides. We anticipate that current work will stimulate large-scale unitization of the HTP experimental technique in the thermoelectric field, which can greatly accelerate the research and development of new high-performance thermoelectric materials.
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http://dx.doi.org/10.34133/2020/1736798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080995PMC
March 2020

Design of C N -Based Hybrid Heterojunctions for Enhanced Photocatalytic Hydrogen Production Activity.

ChemSusChem 2020 Mar 3;13(5):876-881. Epub 2020 Feb 3.

Key Laboratory of Green Perovskites Application of Fujian Province Universities, Fujian Jiangxia University, Fuzhou, 350108, P.R. China.

Semiconductors and metals can form an Ohmic contact with an electric field pointing to the metal, or a Schottky contact with an electric field pointing to the semiconductor. If these two types of heterojunctions are constructed on a single nanoparticle, the two electric fields may cause a synergistic effect and increase the separation rate of the photogenerated electrons and holes. Metal Ni and Ag nanoparticles were successively loaded on the graphitic carbon nitride (g-C N ) surface by precipitation and photoreduction in the hope of forming hybrid heterojunctions on single nanoparticles. TEM/high-resolution TEM images showed that Ag and Ni were loaded on different locations on C N , which indicated that during the photoreduction reaction Ag obtained electrons from C N in the reduction reaction, whereas oxidation reactions proceeded on Ni nanoparticles. Photocatalytic hydrogen production experiments showed that C N -based hybrid heterojunctions can greatly improve the photocatalytic activity of materials. The possible reason is that two heterojunctions could form a long-range electric field similar to the p-i-n structure in semiconductors. Most of the photogenerated carriers were generated and then separated in this electric field, thereby increasing the separation rate of electrons and holes. This further improved the photocatalytic activity of C N .
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http://dx.doi.org/10.1002/cssc.201903437DOI Listing
March 2020

Naringin-loaded polymeric micelles as buccal tablets: formulation, characterization, release, cytotoxicity and histopathology studies.

Pharm Dev Technol 2020 Jun 22;25(5):547-555. Epub 2020 Jan 22.

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, P. R. China.

Naringin (NG) has been proved to have numerous notable biological effects, including anti-inflammatory effect, anti-cancer effect, and anti-ulcer effect, yet there are no clinical preparations of naringin due to its poor solubility and low dissolution rate after oral administration. In this study, in order to overcome these problems, NG was encapsulated into MPEG-PCL micelles (NGMs) by using a thin-film hydration method. NMGs were in a typical core-shell structure, with a mall particle size (23.95 ± 0.51 nm), high drug loading, and encapsulation efficiency. release of NGMs indicated that the dissolution of NG was increased after being encapsulated in the micelles. NGMs were nontoxic in the cytotoxicity and histopathology studies. Furthermore, when the freeze-dried NGMs were compressed into buccal tablets (NGBTs) by direct compression, the release speed of NG under simulated oral cavity condition from NGBTs was higher than the control tablets, with the accumulated dissolution at 93.13% in 8 hours. In conclusion, NGMs and NGBTs represent a promising drug delivery system for NG, which has the potential to improve the current treatment of oral diseases.
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http://dx.doi.org/10.1080/10837450.2020.1715427DOI Listing
June 2020

Preparation, characterization and in vivo evaluation of pharmacological activity of different crystal forms of ibuprofen.

Pak J Pharm Sci 2019 Sep;32(5):2139-2147

Health Science Center, Xi'an Jiaotong University, Xi'an, China.

Different polymorphic forms can affect the performance of the drug product. In addition, isomorphic crystals show different chemical and physical properties due to the changes in the crystal habit. However, it is unclear whether the crystal habit results in different pharmacological activity. The aim of this study was to investigate whether the pharmacological effect of ibuprofen could be affected due to the variety of the crystal habit. Solvent change technique and conventional fusion method were carried out to modify the characteristics of ibuprofen. The physicochemical properties of each were investigated using powder X-ray diffraction (PXRD), Fourier transform infrared (FT-IR) spectroscopy and differential scanning calorimetry (DSC) techniques. Results of scanning electron microscopy (SEM) analysis revealed differences in the surface characteristics of the crystals obtained. Further study revealed that the samples crystallized exhibited the remarkable variation on the dissolution profiles in different dissolution medium. Moreover, in vivo antinociceptive and anti-inflammatory findings demonstrated that the crystal habit modifications resulted in the different therapeutic efficacy. Taken together, these results indicate that the modification of the crystal habit had a great influence on the in vivo pharmacological activity of ibuprofen crystals.
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September 2019

Three-dimensional printed dry lab training models to simulate robotic-assisted pancreaticojejunostomy.

ANZ J Surg 2019 12 6;89(12):1631-1635. Epub 2019 Nov 6.

Department of Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, China.

Background: This pioneering study is aimed to design training models for robotic pancreaticojejunostomy (PJ) and to assess their usefulness using quality improvement exercise in the dry lab.

Methods: Three dry lab models were developed including the anastomosis model of a transected silicon pancreatic stent (model 1), a rough model (model 2) simulating PJ, and an advanced three-dimensional printed model (model 3) more vividly simulating PJ. Three surgeons (A, B, C) with same specialty and levels of expertise in surgery were enrolled in the training which was divided into three rounds of tasks. In the first round, all three surgeons (A, B, C) participated in the training on basic technical tasks before moved on to the next rounds. While surgeons A, B participated in the second round on model 1, only surgeon A worked on model 2 in the third round. Their proficiency of performance was evaluated on model 3.

Results: The results of the first and second rounds between surgeons are similar. Surgeon A practiced with model 2 for 6 h, completing 10 cases. In model 3, the times of attempts before achieving a consecutively three times of satisfactory anastomosis procedures were compared, for surgeon A, six cases, 20 for B and 25 for C.

Conclusions: The specifically designed series of dry lab training models may be a potential training tool for advancing the robotic PJ through quality improvement exercise in dry lab. Further larger and well-designed studies are warranted to validate this issue.
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http://dx.doi.org/10.1111/ans.15544DOI Listing
December 2019

Comparative pharmacokinetics of verapamil and norverapamil in normal and ulcerative colitis rats after oral administration of low and high dose verapamil by UPLC-MS/MS.

Xenobiotica 2020 Jun 31;50(6):713-721. Epub 2019 Oct 31.

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China.

In this study, UC rat model was established by administration of 5% (w/v) dextran sulfate sodium, and the pharmacokinetics of verapamil and norverapamil were evaluated in normal and UC rats using UPLC-MS/MS after oral administration of 5 mg/kg and 50 mg/kg verapamil.The peak concentration (C) and the area under plasma concentration-time curves (AUC) of verapamil in UC rats after oral administration of 5 mg/kg were significantly greater (2.5 times and 2 times, respectively) than those in normal rats, but the clearance rate (Cl) was significantly lower (by 50%). For norverapamil, C and AUC were significantly greater (2.8 times and 2.5 times, respectively), and Cl was significantly lower (by 45%). But, pharmacokinetic parameters of verapamil and norverapamil after oral administration of 50 mg/kg were no significant differences between UC and normal rats.The better absorption and poor excretion for low-dose verapamil may be attributed to down-regulation of P-gp expression in the intestine and kidney. No significant differences of pharmacokinetic parameters for high-dose verapamil may be explained as the saturation of an efflux mechanism.The findings of this study suggested that in UC patients, doses of verapamil should be decreased when low-dose verapamil was orally administrated.
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http://dx.doi.org/10.1080/00498254.2019.1682715DOI Listing
June 2020

[Lumbar facet joint degeneration contributes to degenerative lumbar scoliosis induced by asymmetric stress in rabbits].

Nan Fang Yi Ke Da Xue Xue Bao 2019 Aug;39(8):993-997

Department of Orthopedics and Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To investigate the role of lumbar facet joint degeneration in the development of degenerative lumbar scoliosis caused by asymmetric stress.

Methods: Thirty-six New Zealand white rabbits were randomly divided into 3 groups (n=12): Group A with aspiration of the nucleus pulposus to induce disc degeneration; Group B with removal of the left capsule from the facet joints at L3/4 to L5/6 to induce degeneration; and Group C with both treatments. Springs were deployed on the left adjacent facets at L3/4, L5/6 and L5/6 to stress the facet joints. Serial radiographs were taken at 3 and 6 months, and the facet joint tissues were sampled at 6 months for Safranin O-fast green staining to assess the severity of cartilage degeneration based on the Mankin score.

Results: The Cobb angle differed significantly among the 3 groups (=24.865, =0.000). In all the groups, the Cobb angles at 6 months increased significantly as compared with that at 3 months ( <0.05). The Cobb angles were significantly greater in group C than in the other 2 groups at both 3 and 6 months ( <0.05) but showed no significant difference between Groups A and B (>0.05). The severity of facet joint degeneration also differed significantly among the 3 groups (= 22.009, =0.000), and was the most severe in group C ( <0.05); facet joint degeneration was more severe in group B than in group A ( <0.05).

Conclusions: Facet joint degeneration is an important factor that contributes to the development of degenerative lumbar scoliosis. Disc degeneration and facet joints degeneration can lead to lumbar scoliosis, which in turn aggravates disc degeneration, facet joints degeneration and asymmetric stress, thus forming a vicious circle to further exacerbate lumbar scoliosis.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2019.08.18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765594PMC
August 2019

Thermosensitive hydrogels as a controlled release system for alkannin to improve localized treatment of Candida vaginitis after external beam radiotherapy in vitro and in vivo.

Pathog Dis 2019 06;77(4)

School of Pharmacy, Health Science Center, Xi'an Jiaotong University, No. 76 YantaWestroad, Xi'an, People's Republic of China 710061.

External beam radiotherapy increases the risk of Candida vaginitis in cervical cancer patients, which brings a lot of insufferable influence to their life. Here, we explored the efficacy of alkannin in the treatment of Candida vaginitis after external beam radiotherapy. We exploit thermosensitive hydrogel-mediated alkannin as the topical formulation in a rat model established in our work. Periodic acid-Schiff of vaginas indicated little Candida albicans adhered to the vaginal tissue in treatment group. Additionally, hematoxylin and eosin stain revealed that inflammatory response of high dose alkannin was reduced. Above all, the animal model was first established in our work for the clinical desire. Our results suggested the promising application of alkannin for the disease with satisfying fungicidal activity and anti-inflammatory activity.
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http://dx.doi.org/10.1093/femspd/ftz041DOI Listing
June 2019

miR-182-5p affects human bladder cancer cell proliferation, migration and invasion through regulating Cofilin 1.

Cancer Cell Int 2019 28;19:42. Epub 2019 Feb 28.

1Department of Urology, Hainan General Hospital, Haikou, China.

Background: Human bladder cancer is one of the common malignant tumors, and it mainly occurs in men. miR-182-5p, a member of miR-183 family, acts as tumor suppressor or oncogene in various kinds of tumors. In this study, we first investigate that the absence of miR-182-5p in human bladder cancer promotes tumor growth by regulating the expression of Cofilin 1, an actin modulating-protein.

Methods: Human bladder tumor tissue specimens were collected to detect the expression of miR-182-5p and Cofilin 1 by qRT-PCR. Luciferase activity assay was performed to demonstrate the regulation of Cofilin 1 mRNA 3'UTR by miR-182-5p. Then, cell experiments were performed to analysis the effect of miR-182-5p/Cofilin 1 pathway on tumor cell proliferation, migration, invasion and colony forming efficiency. Finally, xenograft tumor models were established to evaluate the role of miR-182-5p in tumorigenesis abilities in vivo.

Results: qRT-PCR and Western blotting analysis showed that Cofilin 1 expression was up-regulated in both bladder cancer tissues and cell lines compared with normal. Luciferase activity assay showed that miR-182-5p specifically targets Cofilin 1 mRNA 3'UTR and represses the expression of Cofilin 1. Also, miR-182-5p inhibited bladder tumor cell proliferation, migration, invasion and colony forming efficiency. Furthermore, xenograft tumor model assay showed that miR-182-5p plays a negative role in bladder cancer tumorigenesis abilities in vivo.

Conclusion: Present results suggest that miR-182-5p could inhibit human bladder tumor growth by repressing Cofilin 1 expression. Our findings may provide a new horizon for exploring therapeutic target of bladder cancer.
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http://dx.doi.org/10.1186/s12935-019-0758-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394052PMC
February 2019

Hydroxytyrosol NO regulates oxidative stress and NO production through SIRT1 in diabetic mice and vascular endothelial cells.

Phytomedicine 2019 Jan 25;52:206-215. Epub 2018 Sep 25.

Department of Pharmacology, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China. Electronic address:

Background: Vascular complications are major causes of disability and death in people with diabetes mellitus (DM). Nitric oxide (NO) supplement may help prevent vascular complications and is an attractive treatment option for DM. Hydroxytyrosol (HT) is a major polyphenol in olive oil. It is mainly used as a dietary supplement because of its antioxidant effect.

Purpose: We aimed to determine the effects of hydroxytyrosol nitric oxide (HT-NO) on oxidative stress and NO level as well as related mechanisms.

Study Design/methods: The effects of HT-NO on oxidative stress and NO level were examined by using diabetic mouse model and HUVECs.

Results: Our results showed that HT-NO has antioxidant and NO-releasing activities in vitro and in DM mice. HT-NO not only decreased blood glucose and oxidative stress but also increased NO level and deacetylase Sirtuin 1 (SIRT1) expression in DM mice and high glucose (HG)-stimulated HUVECs. Further studies found that SIRT1 activation augmented the effect of HT-NO on eNOS phosphorylation in HG-stimulated HUVECs. However, the promotive effect of HT-NO on eNOS phosphorylation was abolished by SIRT1 knockdown. Most importantly, HT-NO inhibited reactive oxygen species (ROS) production through SIRT1 in HUVECs. The ROS scavenger enhanced the effect of HT-NO on eNOS phosphorylation.

Conclusion: These results suggest that HT-NO regulates oxidative stress and NO production partly through SIRT1 in DM mice and HG-stimulated HUVECs.
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http://dx.doi.org/10.1016/j.phymed.2018.09.208DOI Listing
January 2019

Interaction of long-chain non-coding RNAs and important signaling pathways on human cancers (Review).

Int J Oncol 2018 Dec 27;53(6):2343-2355. Epub 2018 Sep 27.

Department of Hepatobiliary and Pancreatic Surgery and Minimally Invasive Surgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310014, P.R. China.

Long non-coding RNAs (lncRNAs) usually refer to non-coding RNA transcripts >200 nucleotides in length. In terms of the full genomic transcript, the proportion of lncRNAs far exceeds that of coding RNA. Initially, lncRNAs were considered to be the transcriptional noise of genes, but it has since been demonstrated that lncRNAs serve an important role in the regulation of cellular activities through interaction with DNA, RNA and protein. Numerous studies have demonstrated that various intricate signaling pathways are closely related to lncRNAs. Here, we focus on a large number of studies regarding the interaction of lncRNAs with important signaling pathways. It is comprehensively illustrated that lncRNAs regulate key metabolic components and regulatory factors of signaling pathways to affect the biological activities of tumor cells. Evidence suggests that the abnormal expression or mutation of lncRNAs in human tumor cells, and their interaction with signaling pathways, may provide a basis and potential target for the diagnosis and treatment of human cancers.
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http://dx.doi.org/10.3892/ijo.2018.4575DOI Listing
December 2018

High fluorescent sulfur regulating graphene quantum dots with tunable photoluminescence properties.

J Colloid Interface Sci 2018 Nov 7;529:205-213. Epub 2018 Jun 7.

Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, PR China. Electronic address:

Sulfur-doped graphene quantum dots (S-GQDs) were synthesized by two facile hydrothermal technologies. The photoluminescence (PL) properties of the GQDs and S-GQDs samples were mainly investigated. Through regulating the content of S powders in S-GQDs synthesizing process, the optimal S-GQDs have a high S/C atomic ratio of 19.53%. The S doping introduce more functional groups on the C sp skeleton of S-3 sample and result in the appearance of the strong absorption band in the UV region. In comparison with other reported S-GQDs, the S-GQDs exhibit overwhelming high fluorescence quantum yield (57%) and excitation-independent emission, resulting from the outcome of the doped sulfur atoms. Moreover, the PL intensity of GQDs can be increased by doping it with S and the increasing efficiency depends on the thiophene sulfur content.
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http://dx.doi.org/10.1016/j.jcis.2018.06.016DOI Listing
November 2018

Protective effect of dihydromyricetin on LPS-induced acute lung injury.

J Leukoc Biol 2018 Mar 9. Epub 2018 Mar 9.

Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, P. R. China.

Dihydromyricetin (DHM), a bioactive flavonoid component isolated from Ampelopsis grossedentata, is known to have anti-inflammatory effect, but the effect of DHM on acute lung injury (ALI) is largely unknown. Here, we investigated the effect of DHM on ALI and the underlying mechanism by bioinformatic analyses and animal experiments. We found that pretreatment with DHM ameliorated lung pathological changes and suppressed the inflammation response in lung tissues after LPS challenge. The potential targets of DHM were predicted by DDI-CPI and DRAR-CPI tools and analyzed using the STRING server to predict the functionally related signaling pathways, such as MAPK signaling. Molecular docking calculations indicated that DHM could be embedded tightly into the binding pocket of ERK, JNK, and p38. Furthermore, the activation of MAPK signaling induced by LPS was inhibited by DHM. In conclusion, these findings suggest that DHM may exert its protective effect on ALI by inhibiting MAPK signaling. The present study supports a potential clinical application for DHM in treating ALI and provides a novel design that combines in silico methods with in vivo experiments for drug research.
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http://dx.doi.org/10.1002/JLB.3MA0317-101RRRDOI Listing
March 2018

A Rapid Colorimetric Method to Visualize Protein Interactions.

Chemistry 2018 May 17;24(26):6727-6731. Epub 2018 Apr 17.

College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, P. R. China.

As key molecules in most biological pathways, proteins physically contact one or more biomolecules in a highly specific manner. Several driving forces (i.e., electrostatic and hydrophobic) facilitate such interactions and a variety of methods have been developed to monitor these processes both in vivo and in vitro. In this work, a new method is reported for the detection of protein interactions by visualizing a color change of a cyanine compound, a supramolecule complex of 3,3-di-(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (MTC). Nuclear magnetic resonance (NMR) studies suggest that the hydrophobic nature of the protein surfaces drives MTC into different types of aggregates with distinct colors. When proteins interact with other biomolecules, the hydrophobic surface of the complex differs, resulting in a shift in the form of MTC aggregation, which results in a color change. As a result, this in vitro method has the potential to become a rapid tool for the confirmation of protein-biomolecule interactions, without the requirements for sophisticated instrumentation or approaches.
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http://dx.doi.org/10.1002/chem.201800401DOI Listing
May 2018

SIRT1 inhibition promotes atherosclerosis through impaired autophagy.

Oncotarget 2017 Aug 8;8(31):51447-51461. Epub 2017 May 8.

Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi, P. R. China.

SIRT1, a highly conserved NAD-dependent protein deacetylase, plays a pivotal role in the pathogenesis and therapy of atherosclerosis (AS). The aim of this study is to investigate the potential effects of SIRT1 on AS in ApoE mice and the underlying mechanisms of autophagy in an ox-LDL-stimulated human monocyte cell line, THP-1. , the accelerated atherosclerotic progression of mice was established by carotid collar placement; then, mice were treated for 4 weeks with a SIRT1-specific inhibitor, EX-527. The atherosclerotic lesion size of EX-527-treated mice was greatly increased compared to that of the mice in the control group. Immunostaining protocols confirmed that the inhibition of SIRT1 during plaque initiation and progression enhanced the extent of intraplaque macrophage infiltration and impaired the autophagy process. cultured THP-1 macrophages exposed to ox-LDL were utilized to study the link between the SIRT1 function, autophagy flux, pro-inflammatory cytokine secretion, and foam cell formation using different methods. Our data showed that ox-LDL markedly suppressed SIRT1 protein expression and the autophagy level, while it elevated the MCP-1 production and lipid uptake. Additionally, the application of the SIRT1 inhibitor EX-527 or SIRT1 siRNA further attenuated ox-LDL-induced autophagy inhibition. In conclusion, our results show that the inhibition of SIRT1 promoted atherosclerotic plaque development in ApoE mice by increasing the MCP-1 expression and macrophage accumulation. In particular, we demonstrate that blocking SIRT1 can exacerbate the acetylation of key autophagy machinery, the Atg5 protein, which further regulates the THP-1 macrophage-derived foam cell formation that is triggered by ox-LDL.
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http://dx.doi.org/10.18632/oncotarget.17691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584260PMC
August 2017

Preparation, characterization and toxicity evaluation of amphotericin B loaded MPEG-PCL micelles and its application for buccal tablets.

Appl Microbiol Biotechnol 2017 Oct 3;101(19):7357-7370. Epub 2017 Sep 3.

Department of Pharmacology, Health Science Center, Xi'an Jiaotong University, No. 76 Yanta Westroad, Xi'an, 710061, People's Republic of China.

Oral candidiasis or thrush is a fungal infection due to Candida albicans, causing discomfort in areas inside mouth or tongue. The clinical application of antifungal reagent amphotericin B (AMB), which is believed to offer a better treatment for oral candidiasis, is greatly compromised by its toxicities (mainly nephrotoxicity) and poor solubility. In order to overcome these issues, we characterized AMB-loaded MPEG-PCL micelles in vitro and in vivo. In addition, the antifungal activities of AMB/MPEG-PCL micelles-loaded buccal tablet were also evaluated in vitro. We found that micelles system could significantly improve the solubility of AMB yet reduce the overall toxicity, while the buccal tablet system is capable to suppress C. albicans biofilm formation. Furthermore, the toxicity of the buccal tablet system is also reduced compared with other standard preparations. Therefore, the prepared tablet with AMB-loaded MPEG-PCL micelles as oral topical preparations has the potential to improve current treatment of superficial oral C. albicans infections.
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http://dx.doi.org/10.1007/s00253-017-8463-6DOI Listing
October 2017

Hydroxytyrosol regulates the autophagy of vascular adventitial fibroblasts through the SIRT1-mediated signaling pathway.

Can J Physiol Pharmacol 2018 Jan 3;96(1):88-96. Epub 2017 Aug 3.

c Department of Pharmacology, Xi'an Jiaotong University Health Science Center Xi'an, Shaanxi 710061, China.

Hydroxytyrosol (HT), a phenolic compound in olive oil, exerts an anti-inflammatory effect in cardiovascular diseases. Recent studies found that autophagy was a therapeutic target of diseases. However, the effect of HT on autophagy in vascular adventitial fibroblasts (VAFs) remains unknown. Thus, in this study, we aimed to determine the effect of HT on cell autophagy and related signaling pathway and whether HT regulates the inflammatory response through autophagy in VAFs. Our results showed that HT promoted cell autophagy by increasing the conversion of LC3 and Beclin1 expression and the autophagic flux in VAFs stimulated with tumor necrosis factor-α (TNF-α). HT also upregulated the expression of the deacetylase sirtuin 1 (SIRT1) protein and mRNA compared with the TNF-α group. The molecular docking studies showed the good compatibility between HT and SIRT1, indicating that HT might act through SIRT1. Further study found that HT regulated autophagy through SIRT1-mediated Akt/mTOR suppression in VAFs. In addition, HT inhibited TNF-α-induced inflammatory response in VAFs through SIRT1. Furthermore, the study showed that HT inhibited the inflammatory response of VAFs through autophagy. These findings indicate that HT regulates the autophagy of VAFs through SIRT1-mediated Akt/mTOR suppression and then inhibits the inflammatory response of VAFs.
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http://dx.doi.org/10.1139/cjpp-2016-0676DOI Listing
January 2018

Preparation, characterization, and evaluation of amphotericin B-loaded MPEG-PCL-g-PEI micelles for local treatment of oral .

Int J Nanomedicine 2017 6;12:4269-4283. Epub 2017 Jun 6.

Department of Pharmacology.

Fatal infections in the mucosal system can occur in association with immune-compromised diseases and dysbacteriosis. Currently, amphotericin B (AmB) is considered to be the most effective antibiotic in the treatment of infections, but its clinical application is limited by side effects and poor bioavailability. In order to use AmB in the local treatment of oral infections, AmB/MPEG-PCL-g-PEI (monomethoxy poly(ethylene glycol)-poly(epsilon-caprolactone)-graft-polyethylenimine, MPP) micelles were prepared. A series of characterizations were performed. The micelles allowed a sustained in vitro release in both normal oral conditions (pH 6.8) and infection conditions (pH 5.8). Then, buccal tablets containing freeze-dried powder of AmB/MPP micelles were produced by direct compression process and evaluated as regards to weight variation, hardness, and friability. In vitro drug release of the buccal tablets was measured in both the United States Pharmacopeia dissolution apparatus and the dissolution rate test apparatus, which was previously designed for simulating in vivo conditions of the oral cavity. The buccal tablets could sustainably release within 8 h and meet the antifungal requirements. Regarding safety assessment of AmB/MPP micelles, in vivo histopathological data showed no irritation toward buccal mucosa of the rats in both optical microscopy and ultrastructure observation of the tissues. MTT experiment proved that AmB/MPP micelles reduced the cytotoxicity of AmB. The micelles delivered through the gastrointestinal route were also found to be non-systemic toxicity by liquid chromatography-mass spectrometry analysis. Furthermore, the antifungal action of AmB/MPP micelles was evaluated. Although AmB/MPP had no obvious improvement as compared to AmB alone in the antifungal effect on planktonic , the micelles significantly enhanced the antifungal activity against the biofilm state of . Thus, it was concluded that AmB/MPP micelles represent a promising novel drug delivery system for the local treatment of oral infections.
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http://dx.doi.org/10.2147/IJN.S124264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5473597PMC
October 2017

Retraction: Synthesis and facet-dependent photocatalytic activity of BiOBr single-crystalline nanosheets.

Chem Commun (Camb) 2017 04;53(35):4861

Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, P. R. China.

Retraction of 'Synthesis and facet-dependent photocatalytic activity of BiOBr single-crystalline nanosheets' by Wenwen Lin et al., Chem. Commun., 2014, DOI: 10.1039/c3cc41498a
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http://dx.doi.org/10.1039/c7cc90134hDOI Listing
April 2017

Enhanced Average Thermoelectric Figure of Merit of the PbTe-SrTe-MnTe Alloy.

ACS Appl Mater Interfaces 2017 Mar 3;9(10):8729-8736. Epub 2017 Mar 3.

School of Materials Science and Engineering, Shanghai University , 99 Shangda Road, Shanghai 200444, China.

Thermoelectric properties of Na-doped PbTe-SrTe system have been improved by the addition of Mn. The substitution of Mn for Pb modifies the band structure of the PbTe-SrTe alloy, which enlarges the band gap and increases the valence band degeneracy. This leads to increased thermopowers and power factors near room temperature, and the electronic contribution to the total thermal conductivity is also substantially reduced due to increased resistivity. Moreover, alloying of MnTe within the PbTe matrix introduces low angle grain boundaries, and significantly reduces the lattice thermal conductivity due to the dislocation scattering. A thermoelectric figure of merit as high as 1.98 and an enhancement of the average thermoelectric figure of merit by 18% are achieved for the sample with 4 at% Mn with respect to the Mn-free sample, which can be mainly attributed to the synergistic effects of the band structure modification and the dislocation scattering on phonon transport, both induced by alloying with MnTe. Our experimental results demonstrate the promising potential of PbTe-SrTe-MnTe system for the application of waste heat recovery.
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http://dx.doi.org/10.1021/acsami.6b16060DOI Listing
March 2017
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