Publications by authors named "Jiyang Li"

119 Publications

Antifungal effect of photodynamic therapy mediated by curcumin on Candida albicans biofilms in vitro.

Photodiagnosis Photodyn Ther 2019 Sep 21;27:280-287. Epub 2019 Jun 21.

Institution: Department of Stomatology, Huashan Hospital, Fudan University, No.12, Rd. Wulumuqi, Shanghai, China. Electronic address:

Background: Canida albicans can cause opportunistic infections ranging from superficial mucous membrane lesions to life-threatening disease. The aim of this study is to investigate the antifungal effect of photodynamic therapy (PDT) mediated by curcumin (CUR) on C. albicans biofilms in vitro.

Methods: One standard strain ATCC 90028 and two clinical isolates from HIV (CCA1) and oral lichen planus (CCA2) patients' oral cavities were used in this study. Biofilms were photosensitized with 60 μM CUR and irradiated by light emitting diode (LED) under the wavelength of 455 nm and energy densities of 2.64, 5.28, 7.92, 10.56, 13.2 J/cm. Then the antifungal effects of CUR-PDT were evaluated by XTT reduction assay and confocal light scanning microscopy (CLSM) observations. The effects of CUR-PDT on the expression levels of hypha-specific and biofilm-related genes including EFG1, UME6, HGC1 and ECE1 were assessed by quantitative Real-time PCR (qRT-PCR) method.

Results: The inhibition rates after CUR-PDT in three biofilms(ATCC 90028, CCA1, CCA2)were 90.87%, 66.44% and 86.74% respectively (p < 0.05). Relative gene expression levels of EFG1, UME6, HGC1 and ECE1 were all downregulated after CUR-PDT, with fold-decrease of 6.865, 3.382, 2.167 and 6.887 in ATCC 90028, 2.466, 2.146, 1.627 and 3.102 in CCA1, and 5.406, 2.347, 2.073and 3.711 in CCA2 (p < 0.05).

Conclusions: Curcumin-mediated PDT could effectively inactivateCandida albicans biofilms in vitro. Expression of genes involved in biofilms formation were downregulated after CUR-PDT.
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http://dx.doi.org/10.1016/j.pdpdt.2019.06.015DOI Listing
September 2019

Red Room-Temperature Phosphorescence of [email protected] Composites Triggered by Heteroatoms in Zeolite Frameworks.

ACS Cent Sci 2019 Feb 22;5(2):349-356. Epub 2019 Jan 22.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Carbon dots (CDs) with red-emitting room-temperature phosphorescence (RTP) are rarely reported because of the increasing nonradiative decay of the excited states and the decreasing energy gap between the excited states and ground states. Herein, we demonstrate a facile strategy for modulating the RTP properties of CDs in terms of donor-acceptor energy transfer (EnT) in the CDs-in-zeolite system. Upon tuning of the heteroatoms (Zn, Mn) doped in the aluminophosphate zeolite frameworks, [email protected] composites with green and red phosphorescence have been prepared via in situ hydrothermal synthesis. In such composites, the zeolite matrix provides an efficient confinement role in stabilizing the triplet states of CDs. Significantly, the Mn-doped zeolite could act as an energy acceptor allowing EnT from excitons of CDs to the dopant in the host matrix, generating the intriguing red RTP behavior. This work provides an effective strategy for developing CD-based composite materials with special RTP emissions as well as new fields for applications.
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http://dx.doi.org/10.1021/acscentsci.8b00844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396386PMC
February 2019

Long noncoding RNA regulates tumor cell proliferation and invasion by epithelial-mesenchymal transition in gastric cancer.

Therap Adv Gastroenterol 2019 19;12:1756284819827697. Epub 2019 Feb 19.

Department of General Surgery & Institute of General Surgery, Chinese People's Liberation Army General Hospital, Fuxing Road 28, Beijing 100853, PR China.

Background: The clinical relevance and biological role of tissular in gastric cancer (GC) remains to be clarified.

Methods: The association between expression and clinicopathological characteristics was investigated. , 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, wound healing and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed to explore the biological effects of on GC cell proliferation, migration, invasion, and apoptosis in MGC-803 and BGC-823 cell lines. , animal experiments were conducted to confirm the findings. Quantitative real-time polymerase chain reaction, western blotting, and immunofluorescence were used to investigate the potential mechanisms.

Results: Expression levels of were significantly higher in tumor tissues than in noncancerous tissues, and patients with high levels of had poor overall and disease-free survival. expression was correlated with lymphovascular invasion. , knockdown of inhibited tumor cell proliferation, migration, and invasion, and promoted apoptosis of MGC-803 and BGC-823 cells. , BGC-823 cells transfected with siRNA formed smaller and lighter tumors than BGC-823 cells transfected with negative control siRNA in severe combined immunodeficiency mice. Additionally, the si- group had less proliferating cells and more apoptotic cells in tumor xenografts compared with the negative control. Mechanistically, knockdown of decreased the expression of vimentin and MMP9, while increasing the expression of E-cadherin. Immunofluorescence confirmed the relationship between expression and E-cadherin, vimentin, and MMP9 levels in clinical GC specimens.

Conclusions: promotes tumorigenesis and progression partly through epithelial-mesenchymal transition in GC. Additionally, may serve as a prognostic biomarker for clinical decision making.
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http://dx.doi.org/10.1177/1756284819827697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383096PMC
February 2019

Carbon Dots-in-Matrix Boosting Intriguing Luminescence Properties and Applications.

Small 2019 08 13;15(32):e1805504. Epub 2019 Feb 13.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun, 130012, P. R. China.

As a new class of luminescent nanomaterials, carbon dots (CDs) have aroused significant interest because of their fascinating photoluminescence properties and potential applications in biological, optoelectronic, and energy-related fields. Strikingly, embedding CDs in host matrices endow them with intriguing luminescent properties, in particular, room temperature phosphorescence and thermally activated delayed fluorescence, due to the confinement effect of the host matrix and the H-bonding interactions between CDs and the matrix. Here, the state-of-the-art strategies for introducing CDs in various host matrices are summarized, such as nanoporous materials, polyvinyl alcohol, polyurethane, potash alum, layered double hydroxides, amorphous silica, etc. The resultant luminescent properties of the composites and their emission mechanisms are discussed. Their applications in bioimaging, drug delivery/release, sensing, and anticounterfeiting are also presented. Finally, current problems and challenges of CDs-based composites are noted for future development of such luminescent materials.
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http://dx.doi.org/10.1002/smll.201805504DOI Listing
August 2019

A novel hybrid of 3-benzyl coumarin seco-B-ring derivative and phenylsulfonylfuroxan induces apoptosis and autophagy in non-small-cell lung cancer.

Phytomedicine 2019 Jan 26;52:79-88. Epub 2018 Sep 26.

Minghang Hospital & Department of Microbiological and Biochemical Pharmacy at School of Pharmacy, Fudan University, Shanghai 201199, China. Electronic address:

Background: Compound 6, as a novel hybrid of 3-benzyl coumarin seco-B-ring derivative and nitric oxide (NO) donor phenylsulfonylfuroxan, has the potential to develop into an anticancer drug because it displays significant antiproliferation activitity for various solid cancer cell lines including non-small-cell lung cancer (NSCLC) cells.

Purpose: We attempt to uncover the capacities of compound 6 to induce apoptosis and autophagy in NSCLC cells, as well as the underlying mechanism involved in this process.

Methods: The effect of compound 6 on cell viability was evaluated in A549 cells by MTT assay. Apoptosis was mainly detected by flow cytometry. The induction of autophagy was observed by transmission electron microscopy (TEM), confocal microscopy as well as western-blotting technique. The expression of all related-proteins including PI3K/Akt/mTOR signaling pathway were also examined by western-blotting technique.

Results: Above all, distinct growth inhibition and caspase-dependent apoptosis were detected in A549 cells administered with compound 6. Then, we confirmed the induction of autophagy triggered by compound 6 in A549 cells. Noticeably, blocking autophagy using a series of inhibitors and ATG5 siRNA had little effect on the cytotoxicity of compound 6, elucidating nonprotective autophagy triggered in NSCLC cells. Further research illustrated that PI3K/Akt/mTOR signaling pathway was involved in compound 6-induced apoptosis, and 3-MA as well as LY294002 had synergistic inhibiting effect on proliferation of A549 cells through the pathway mentioned above.

Conclusion: These findings raise a rationale that this 3-benzyl coumarin seco-B-ring derivative and phenylsulfonylfuroxan hybrid could be a promising candidate for developing as a therapeutic agent toward NSCLC, and the combination therapy through PI3K/Akt/mTOR signaling pathway may result in optimized treatment outcomes.
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http://dx.doi.org/10.1016/j.phymed.2018.09.216DOI Listing
January 2019

Randomized controlled trial comparing short-term outcomes of laparoscopic and open spleen-preserving splenic hilar lymphadenectomy for advanced proximal gastric cancer: An interim report.

J Surg Oncol 2018 Dec 31;118(8):1264-1270. Epub 2018 Oct 31.

Department of General Surgery, Chinese PLA General Hospital, China.

Background And Objectives: We compared the clinical outcomes of laparoscopic and open spleen-preserving splenic hilar lymphadenectomy (LSPL and OSPL) for gastric cancer.

Methods: We performed a single-center, randomized, controlled trial to compare the short-term surgical outcomes between LSPL and OSPL. The study was registered in ClinicalTrials.gov (NCT02980861).

Results: A total of 222 patients were enrolled (114 in the LSPL group and 108 in the OSPL group). There were no significant differences between the two groups in operative time (P = 0.152), a number of harvested lymph nodes (P = 0.669) including no. 10 lymph nodes (2.1 ± 1.4 vs 2.3 ± 1.2, P = 0.713). The time taken for no. 10 lymph node dissection was similar in both groups (13.9 ± 10.4 vs 15.2 ± 9.4 minutes, P = 0.217); however, the LSPL group experienced less total blood loss (P < 0.001) and less blood loss during no. 10 lymph node dissection compared with the OSPL group (15.3 ± 37.8 vs 29.5 ± 36.4 mL, P < 0.001). The postoperative complication rates of LSPL and OSPL were 18.3% and 16.1%, respectively (P = 0.331).

Conclusion: LSPL is a safe and feasible surgical procedure in no. 10 LN dissection for patients with advanced proximal gastric cancer. Thus, this prospective trial is continuing.
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http://dx.doi.org/10.1002/jso.25262DOI Listing
December 2018

Depth of focus extension by filtering in the frequency domain in laser frequency-shifted feedback imaging.

Appl Opt 2018 Jul;57(20):5823-5830

The depth of focus extension in optical imaging is of considerable interest. In this paper, a laser frequency-shifted feedback scanning imaging configuration is demonstrated whose depth of focus is greatly extended through numerical filtering. The transmission characteristics of the system are studied. The original image is acquired through a two-dimensional scanning point by point with the target placed on a defocused plane. Filtered in the frequency domain, images on any oriented plane can be refocused. The superior performances are presented by imaging a three-dimensional target, and the process of gradual refocusing is demonstrated. To obtain the maximum extension in the depth of defocus, a series of numerical experiments has been carried out, which reveals its depth of focus is capable of being extended to four times the length of the objective focus length. The fabulous performances can motivate three-dimensional surface profile measurement.
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http://dx.doi.org/10.1364/AO.57.005823DOI Listing
July 2018

Comparison of robotic- and laparoscopic-assisted gastrectomy in advanced gastric cancer: updated short- and long-term results.

Surg Endosc 2019 02 16;33(2):528-534. Epub 2018 Jul 16.

Department of General Surgery, Chinese People's Liberation Army General Hospital, Fuxing Road 28, Beijing, 100853, People's Republic of China.

Background: Emerging evidence has demonstrated that either laparoscopic-assisted gastrectomy (LAG) or robotic-assisted gastrectomy (RAG) could be adopted as standard treatment for early gastric cancer. However, the long-term survival and recurrence rate after LAG or RAG for locally advanced gastric cancer (AGC) has seldom been reported.

Methods: We retrospectively analyzed the data from 339 patients who underwent LAG and 163 patients who underwent RAG from a prospectively established database in the Chinese People's Liberation Army General Hospital. We compared the short- and long-term oncological outcomes of the RAG group versus the LAG group in the entire cohort, and in a propensity score-matched cohort.

Results: Before propensity score matching (PSM), the two groups revealed comparable 3-year overall survival rates (OS, RAG vs. LAG: 76.1 vs. 81.7%, p = 0.118), and recurrence-free survival rates (RFS, RAG vs. LAG: 73.0 vs. 67.6%, p = 0.297). Similar results were obtained in the propensity score-matched cohort; the respective overall survival rates in the propensity score-matched RAG and LAG groups were 76.1 and 79.8% (p = 0.552), and the respective RFS rates were 73.0 and 68.7% (p = 0.386). After PSM, RAG was still associated with a significantly longer mean operating time (249.46 ± 63.26 vs. 232.17 ± 65.39 min, p = 0.008) and higher total costs (133.38 ± 41.62 vs. 95.34 ± 29.39 10 RMB, p < 0.001) than LAG; the two groups did not significantly differ in other surgical and oncological characteristics.

Conclusion: Although there were some differences in the outcomes of RAG versus LAG in AGC patients, both RAG and LAG were similar in short-term recovery and long-term oncological outcomes.
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http://dx.doi.org/10.1007/s00464-018-6327-5DOI Listing
February 2019

Electrochemical Degradation Mechanism and Thermal Behaviors of the Stored LiNiCoMnO Cathode Materials.

ACS Appl Mater Interfaces 2018 Aug 18;10(30):25454-25464. Epub 2018 Jul 18.

Department of Chemistry, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering , Xiamen University , Xiamen 361005 , China.

The degradation mechanism of the stored LiNiCoMnO (NCM523) electrode has been systematically investigated by combining physical and electrochemical tests. After stored at 55 °C and 80% relative humidity for 4 weeks, the NCM523 materials are coated with a layer of impurities containing adsorbed species, LiCO and LiOH, resulting in both the weight gains of the materials and the electrochemical performance deterioration of the electrode. The impurities generated in air will react with the electrolyte and instantly turn into Li PO F and other species containing the decomposition products of electrolyte when the stored NCM523 materials are soaked into the electrolyte, causing the charge potential plateau and the impedance to ascend. For the stored NCM523 electrodes, the huge and changeable impedance deteriorates the discharge capacity in the first 10 cycles and the discharge capacity will slowly recover and stabilize within 10 cycles when charging/discharging in 0.1 or 0.2 C. The thermal stability of the stored NCM523 materials get slightly better due to the relatively lower delithiated state after charged to 4.3 V.
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http://dx.doi.org/10.1021/acsami.8b07873DOI Listing
August 2018

Formation and origin of multicenter photoluminescence in zeolite-based carbogenic nanodots.

Nanoscale 2018 Jun 30;10(22):10650-10656. Epub 2018 May 30.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Carbogenic nanodots (CNDs) are inspiring intensive research efforts owing to their excellent optical properties; however, their chemical structures and photoluminescent origins are still under study. Herein, CNDs with interesting multicenter photoluminescence (PL) have been successfully prepared by pyrolysis of organo-templated zeolites. The simultaneously observed excitation-dependent blue and excitation-independent green PL emissions as well as an uncommon ultraviolet-excited cyan emission are quite different from those of the known multicenter emissive CNDs. To get a thorough understanding of the formation of these CNDs and their PL origins, structural characterizations coupled with femtosecond transient absorption and fluorescence lifetime spectra analyses have been employed to study such PL behavior. The results show that the as-prepared CNDs consist of three main batches with different polarities, and they are composed of a combination of six small fluorophores with diverse ratios. The multicenter PL is obtained due to competition between the conjugated domain fluorophores and the surface-occupied functional group fluorophores. This study provides a comprehensive insight into the origin of multicenter photoluminescence in zeolite-based CNDs.
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http://dx.doi.org/10.1039/c8nr02043dDOI Listing
June 2018

[Comparison of clinicopathological features and prognosis analysis between gastric stump cancer and recurrence of gastric cancer after radical gastrectomy].

Zhonghua Wei Chang Wai Ke Za Zhi 2018 May;21(5):507-513

Department of General Surgery, Chinese People's Liberation Army General Hospital, Beijing 100853,China.

Objective: To investigate and compare the clinicopathological characteristics of gastric stump cancer(GSC) and the recurrent of gastric cancer (RGC) following radical gastrectomy, and to evaluate survival prognosis.

Methods: A retrospective cohort study was performed on clinicopathological and survival data of patients with GSC (n=31) and with RGC (n=105) following radical gastrectomy at the Chinese People's Liberation Army General Hospital between January 1992 and August 2017. GSC was defined as cancer occured in remnant stomach ≥10 years after radical gastrectomy, while RGC was defined as <10 years. Patients of both groups received radical resection or palliative operation with tumor resection and had complete clinicopathological data regarding the first operation and gastric stump operation. T-test was used to compare quantitative data between the two groups, and Pearson χ test was used to compare qualitative data between the two groups. Kaplan-Meier method was applied to draw survival curves and log-rank test to assess survival differences.

Results: Of the 136 enrolled patients, 113 were male and 23 were female. In the first operation, compared with RGC group, in GSC group, the Borrmann type and histological differentiation were more better [Borrmann I(: 11/31 (35.5%) vs. 5/105 (4.8%), χ=23.003, P=0.001; the high differentiation: 15/31 (48.4%) vs. 1/105 (1.0%), χ=57.137, P=0.001]; the tumor diameter was smaller [<4 cm: 28/31(90.3%) vs. 56/105(53.3%), χ=14.045, P=0.001]; the pT stage [pT1: 12/31 (38.7%) vs. 3/105 (2.9%), χ=50.373, P=0.001], pN stage [pN0: 28/31 (90.3%) vs. 19/105 (18.1%), χ=55.722, P=0.001] and pTNM staging [I(: 26/31 (83.9%) vs. 11/105 (10.5%), χ=66.688, P=0.001] were earlier. Most of the GSC occurred at non-anastomotic sites, while the recurrence mostly occurred at anastomotic sites [51.6%(16/31) vs. 61.9%(65/105), χ=7.520, P=0.023]. Compared with RGC group, GSC group had better histological differentiation [high differentiation: 5/31 (16.1%) vs. 2/105(1.9%), χ=10.029, P=0.007]. There was more histological type change between the first and the second operation in GSC group than that in RGC group[48.4%(15/31) vs. 26.7%(28/105), χ=5.222, P=0.022]. The overall survival time of GSC group was significantly longer than that of RGC group [mean: (161.0±18.6) months vs. (50.8±27.6) months, respectively, Log-rank: 76.818, P=0.001]. The survival time after the second surgery of GSC group was longer than that of RGC group [mean: (30.7±18.4) months vs. (20.5±15.0) months, P=0.003]. In the subgroup analysis of all the 136 patients according to histological type change between the two surgeries (unchanged 93 patients, changed 43 patients), compared with unchanged group, the overall survival time of changed group was longer [mean: (99.6±56.5) months vs. (72.1±58.1) months, P=0.008].

Conclusions: GSC patients have better histological differentiation and earlier clinical stage of primary gastric cancer, and longer survival time compared with RGC patients. The histological type change between two operations may be used as a new factor to define GSC.
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May 2018

Robot-Assisted Versus Laparoscopy-Assisted Proximal Gastrectomy for Early Gastric Cancer in the Upper Location: Comparison of Oncological Outcomes, Surgical Stress, and Nutritional Status.

Cancer Control 2018 Jan-Mar;25(1):1073274818765999

1 Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, China.

Background: An increasing amount of attention has been paid to minimally invasive function-preserving gastrectomy, with an increase in incidence of early gastric cancer in the upper stomach. This study aimed to compare oncological outcomes, surgical stress, and nutritional status between robot-assisted proximal gastrectomy (RAPG) and laparoscopy-assisted proximal gastrectomy (LAPG).

Methods: Eighty-nine patients were enrolled in this retrospective study between November 2011 and December 2013. Among them, 27 patients underwent RAPG and 62 underwent LAPG. Perioperative parameters, surgical stress, nutritional status, disease-free survival, and overall survival were compared between the 2 groups.

Results: Sex, age, and comorbidity were similar in the RAPG and LAPG groups. There were also similar perioperative outcomes regarding operation time, complications, and length of hospital stay between the groups. The reflux esophagitis rates following RAPG and LAPG were 18.5% and 14.5%, respectively ( P = .842). However, patients in the RAPG group had less blood loss ( P = .024), more harvested lymph nodes ( P = .021), and higher costs than those in the LAPG group ( P < .001). With regard to surgical stress, no significant differences were observed in C-reactive protein concentrations and white blood cell count on postoperative days 1, 3, and 7 between the groups ( Ps > .05). There appeared to be higher hemoglobin levels at 6 months ( P = .053) and a higher body mass index at 12 months ( P = .056) postoperatively in patients in the RAPG group compared with those in the LAPG group, but this difference was not significant. Similar disease-free survival and overall survival rates were observed between the groups.

Conclusions: RAPG could be an alternative to LAPG for patients with early gastric cancer in the upper stomach with comparable oncological safety and nutritional status. Further well-designed, prospective, large-scale studies are needed to validate these results.
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http://dx.doi.org/10.1177/1073274818765999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852367PMC
November 2018

An Extra-Large-Pore Zeolite with 24×8×8-Ring Channels Using a Structure-Directing Agent Derived from Traditional Chinese Medicine.

Angew Chem Int Ed Engl 2018 05 22;57(22):6486-6490. Epub 2018 Mar 22.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun, 130012, P. R. China.

Extra-large-pore zeolites have attracted much interest because of their important applications for processing larger molecules. Although great progress has been made in academic science and industry, it is challenging to synthesize these materials. A new extra-large-pore zeolite SYSU-3 (Sun Yat-sen University no. 3) has been synthesized by using a novel sophoridine derivative as an organic structure-directing agent (OSDA). The framework structure was solved and refined using continuous rotation electron diffraction (cRED) data from nanosized crystals. SYSU-3 exhibits a new zeolite framework topology, which has the first 24×8×8-ring extra-large-pore system and a framework density (FD) as low as 11.4 T/1000 Å . The unique skeleton of the OSDA plays an essential role in the formation of the distinctive zeolite structure. This work provides a new perspective for developing new zeolitic materials by using alkaloids as cost-effective OSDAs.
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http://dx.doi.org/10.1002/anie.201801386DOI Listing
May 2018

Carbon Quantum Dots as Fluorescent Probes for Imaging and Detecting Free Radicals in .

J Nanosci Nanotechnol 2018 Feb;18(2):763-771

College of Chemistry, Jilin University, Changchun, Jilin, 130012, China.

Uniform and hydrophilic carbon quantum dots (C-QDs) were synthesized by calcination and NaOH treatment from an organo-templated zeolite precursor. The color of luminescence was determined by the concentration of C-QDs. These variable-color C-QDs were applied for the first time in the imaging of Caenorhabditis elegans (C. elegans) as a model organism. The effects of C-QDs and possible behavioral changes in C. elegans were evaluated under treatment conditions. We could clearly observe distribution of C-QDs in C. elegans from the fluorescence images. Furthermore, we observed significant and detectable fluorescence quenching of the C-QDs by free radicals in C. elegans. Our work affirms that C-QDs can be developed as imaging probes and as potential fluorescent quantitative probes for detecting free radicals.
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http://dx.doi.org/10.1166/jnn.2018.15190DOI Listing
February 2018

A hybrid of coumarin and phenylsulfonylfuroxan induces caspase-dependent apoptosis and cytoprotective autophagy in lung adenocarcinoma cells.

Phytomedicine 2018 Jan 26;39:160-167. Epub 2017 Dec 26.

Department of Microbiological & Biochemical Pharmacy, School of Pharmacy, Fudan University, Shanghai, 201203, China. Electronic address:

Background: Lung adenocarcinoma is the most primary histologic subtype of non-small cell lung cancer (NSCLC). Compound 8b, a novel coumarin derivative with phenylsulfonylfuroxan group, shows significant antiproliferation activity against lung adenocarcinoma cell with low toxicity.

Purpose: This study aims to uncover the potential of compound 8b in relation to apoptosis as well as autophagy induction in lung adenocarcinoma cells.

Study Design: The cytotoxicity and apoptosis of A549 and H1299 cells induced by compound 8b were detected by MTT, microscope and western blot analysis. Autophagy was determined by TEM, confocal microscopy and western blot analysis. Akt/mTOR and Erk signaling pathway were also examined by western blot analysis.

Results: First, significant growth inhibition and caspase-dependent apoptosis were observed in compound 8b-treated A549 and H1299 cells. Then, we confirmed compound 8b-induced autophagy by autophagosomes formation, upregulated expression of autophagy-related protein LC3-II and autophagic flux. Importantly, abolishing autophagy using inhibitors and ATG5 siRNA enhanced the cytotoxicity of compound 8b, indicating the cytoprotective role of autophagy in lung adenocarcinoma. Further mechanistic investigations suggested that Akt/mTOR and Erk signaling pathways contributed to autophagy induction by compound 8b.

Conclusion: This results demonstrate that compound 8b induces caspase-dependent apoptosis as well as cytoprotective autophagy in lung adenocarcinoma cells, which may provide scientific evidence for developing this furoxan-based NO-releasing coumarin derivative as a potential anti-lung adenocarcinoma therapeutic agents.
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http://dx.doi.org/10.1016/j.phymed.2017.12.029DOI Listing
January 2018

A Zwitterionic Ligand-Based Cationic Metal-Organic Framework for Rapidly Selective Dye Capture and Highly Efficient Cr O Removal.

Chemistry 2018 Feb 31;24(11):2718-2724. Epub 2018 Jan 31.

State Key Lab of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun Province, 2699 Qianjin Street, Changchun, 130012, P. R. China.

A cationic metal-organic framework (MOF), [Cu L(H O) ]⋅(NO ) ⋅5.5 H O (1) has been successfully synthesized from a zwitterionic ligand 1,1'-bis(3,5-dicarboxyphenyl)-4,4'-bipyridinium chlorine ([H L]Cl ). The framework of compound 1 contains classical {Cu (O C) } paddlewheels, and possesses typical nbo-type topology and two types of channels with sizes of 5.0 and 15.54 Å. Benefitting from the 3D cationic framework and high pore volume, compound 1 shows interesting selective adsorption ability for anionic dyes. Such material can be successfully employed in a chromatographic column to efficiently separate mixed dyes of Fluorescein Sodium and Methylene Blue. In addition, compound 1 exhibits excellent Cr O removal capacity with maximum adsorption amount of 222.5 mg g , which ranks among the higher Cr O adsorption amounts of MOF materials ever reported, based on ion-exchange. The strategy to construct cationic MOFs based on zwitterionic ligands will promote the development of functional porous materials for the capture and removal of anionic pollutant species from contaminated liquid.
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http://dx.doi.org/10.1002/chem.201705399DOI Listing
February 2018

Shikonin induces ROS-based mitochondria-mediated apoptosis in colon cancer.

Oncotarget 2017 Dec 17;8(65):109094-109106. Epub 2017 Nov 17.

Department of General Surgery, Chinese People's Liberation Army General Hospital, Beijing 100853, China.

Colon cancer is the third most common malignancy worldwide, and chemotherapy is a widely used strategy in clinical therapy. Chemotherapy-resistant of colon cancer is the main cause of recurrence and progression. Novel drugs with efficacy and safety in treating colon cancer are urgently needed. Shikonin, a naphthoquinone derived from the roots of the herbal plant , has been determined to be a potent anti-tumor agent. The aim of the present study was to detect the underlying anti-tumor mechanism of shikonin in colon cancer. We found that shikonin suppressed the growth of colon cancer cells in a dose-dependent manner and . Shikonin induced mitochondria-mediated apoptosis, which was regulated by Bcl-2 family proteins. Shikonin increased the generation of intracellular ROS, which played an upstream role in shikonin-induced apoptosis. Our data indicated that generation of ROS, down-regulated expression of Bcl-2 and Bcl-xL, depolarization of the mitochondrial membrane potential and activation of the caspase cascade were components of the programmed event of shikonin-induced apoptosis in colon cancer cells. In addition, shikonin presented minimal toxicity to non-neoplastic colon cells and no liver injury in xenograft models, showing safety in the control of colon cancer cell growth and . Taken together, our findings suggest that shikonin might serve as a potential novel therapeutic drug in the treatment of human colon cancer.
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http://dx.doi.org/10.18632/oncotarget.22618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5752506PMC
December 2017

Minimally invasive surgery as a treatment option for gastric cancer with liver metastasis: a comparison with open surgery.

Surg Endosc 2018 03 26;32(3):1422-1433. Epub 2017 Oct 26.

Department of General Surgery, Chinese People's Liberation Army General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, China.

Background: As minimally invasive techniques advances, minimally invasive surgery (MIS) has emerged as an alternative modality for advanced gastric cancer. In this study, we compared the short- and long-term surgical outcomes of MIS and conventional open surgery for gastric cancer liver metastasis (GCLM) in terms of safety, feasibility, and efficacy.

Methods: This retrospective study used data from a prospective database at the Chinese People's Liberation Army General Hospital. From January 2006 to June 2016, 53 gastric cancer patients with synchronous liver metastasis accepted radical gastrectomy combined with either or both hepatectomy and radiofrequency ablation for liver metastases. The 53 patients enrolled in the study were divided into two groups: a conventional open surgery group (n = 42) and an MIS group (n = 11). Propensity score matching (PSM) analysis was performed to overcome possible bias.

Results: With PSM performed at a 1:3 ratio, 11 patients who received MIS were compared with 33 open surgery cases. Mean operation time was significantly longer for the MIS group compared with the open surgery group (301 vs. 236 min, P = 0.032), while the open surgery group had a larger estimated blood loss than the MIS group (421 vs. 196 ml, P = 0.019). Time to first flatus and postoperative complications, including Clavien-Dindo classification, were similar in the two groups. However, patients undergoing MIS had a significantly shorter time to first sips of water (P = 0.020) and soft diet (P = 0.020) compared with open surgery counterparts. Long-term outcomes were comparable between groups (P = 0.090) after adjustment by PSM analysis.

Conclusions: MIS achieved superior short-term outcomes and comparable long-term outcomes compared with open surgery in GCLM patients. For experienced surgeons, both laparoscopic and robotic methods of MIS are reasonable approaches for the management of highly selected GCLM patients.
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http://dx.doi.org/10.1007/s00464-017-5826-0DOI Listing
March 2018

Laser diode side-pumped Nd:YVO microchip laser with film-etched microcavity mirrors.

J Opt Soc Am A Opt Image Sci Vis 2017 Oct;34(10):1787-1792

Microchip lasers are applied as the light sources on various occasions with the end-pumping scheme. However, the vibration, the temperature drift, or the mechanical deformation of the pumping light in laser diodes in the end-pumping scheme will lead to instability in the microchip laser output, which causes errors and malfunctioning in the optic systems. In this paper, the side-pumping scheme is applied for improving the disturbance-resisting ability of the microchip laser. The transverse mode and the frequency purity of the laser output are tested. To ensure unicity in the frequency of the laser output, numerical simulations based on Fresnel-Kirchhoff diffraction theory are conducted on the parameters of the microchip laser cavity. Film-etching technique is applied to restrain the area of the film and form the microcavity mirrors. The laser output with microcavity mirrors is ensured to be in single frequency and with good beam quality, which is significant in the applications of microchip lasers as the light sources in optical systems.
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http://dx.doi.org/10.1364/JOSAA.34.001787DOI Listing
October 2017

Evaluation of hepatectomy and palliative local treatments for gastric cancer patients with liver metastases: a propensity score matching analysis.

Oncotarget 2017 Sep 27;8(37):61861-61875. Epub 2017 Jun 27.

Department of General Surgery, Chinese People's Liberation Army General Hospital, Beijing 100853, China.

Background: The optimal treatments for gastric cancer with liver metastases (GCLM) remain controversial. This study aimed to evaluate the efficacy of hepatectomy, RFA and TACE as local treatments for GCLM.

Methods: From 2001 to 2015, 119 consecutive patients who received multidisciplinary treatments based on curative gastrectomy and local treatments (hepatectomy, RFA and TACE) for liver metastases were enrolled in this retrospective cohort study. Patients were divided into Group A (46, hepatectomy) and Group B (73, either or both RFA and TACE). Propensity score matching analysis was employed.

Results: The propensity model revealed that hepatectomy was associated with significantly longer OS compared with either or both RFA and TACE (P=0.021). The 1-, 3- and 5-year OS rates were 80.5%, 41.5% and 24.4%, respectively in Group A; and 85.4%, 21.9% and 12.2%, respectively in Group B. Subgroup analyses indicated that hepatectomy was associated with significantly longer long-term survival compared with TACE (P=0.033) and RFA (P=0.010). TACE had a similar efficacy as RFA (P=0.518), but with significantly lower costs (P=0.014) in for patients with metachronous GCLM.

Conclusion: Hepatectomy is the optimal local treatment for GCLM when surgical R0 resection is intended. TACE attained a similar prognosis as RFA with relatively high cost-effectiveness, particularly for patients with metachronous GCLM.
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http://dx.doi.org/10.18632/oncotarget.18709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5617470PMC
September 2017

High sulfur-containing carbon polysulfide polymer as a novel cathode material for lithium-sulfur battery.

Sci Rep 2017 09 12;7(1):11386. Epub 2017 Sep 12.

State Key Laboratory of Physical Chemistry of Solid Surfaces, State-Province Joint Engineering Laboratory of Power Source Technology for New Energy Vehicle, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, P.R. China.

The lithium-sulfur battery, which offers a high energy density and is environmental friendly, is a promising next generation of rechargeable energy storage system. However, despite these attractive attributes, the commercialization of lithium-sulfur battery is primarily hindered by the parasitic reactions between the Li metal anode and dissolved polysulfide species from the cathode during the cycling process. Herein, we synthesize the sulfur-rich carbon polysulfide polymer and demonstrate that it is a promising cathode material for high performance lithium-sulfur battery. The electrochemical studies reveal that the carbon polysulfide polymer exhibits superb reversibility and cycle stability. This is due to that the well-designed structure of the carbon polysulfide polymer has several advantages, especially, the strong chemical interaction between sulfur and the carbon framework (C-S bonds) inhibits the shuttle effect and the π electrons of the carbon polysulfide compound enhance the transfer of electrons and Li. Furthermore, as-prepared carbon polysulfide polymer-graphene hybrid cathode achieves outstanding cycle stability and relatively high capacity. This work highlights the potential promise of the carbon polysulfide polymer as the cathode material for high performance lithium-sulfur battery.
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http://dx.doi.org/10.1038/s41598-017-11922-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5595856PMC
September 2017

Carbon dots in zeolites: A new class of thermally activated delayed fluorescence materials with ultralong lifetimes.

Sci Adv 2017 May 26;3(5):e1603171. Epub 2017 May 26.

State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China.

Thermally activated delayed fluorescence (TADF) materials are inspiring intensive research in optoelectronic applications. To date, most of the TADF materials are limited to metal-organic complexes and organic molecules with lifetimes of several microseconds/milliseconds that are sensitive to oxygen. We report a facial and general "dots-in-zeolites" strategy to in situ confine carbon dots (CDs) in zeolitic matrices during hydrothermal/solvothermal crystallization to generate high-efficient TADF materials with ultralong lifetimes. The resultant [email protected] composites exhibit high quantum yields up to 52.14% and ultralong lifetimes up to 350 ms at ambient temperature and atmosphere. This intriguing TADF phenomenon is due to the fact that nanoconfined space of zeolites can efficiently stabilize the triplet states of CDs, thus enabling the reverse intersystem crossing process for TADF. Meanwhile, zeolite frameworks can also hinder oxygen quenching to present TADF behavior at air atmosphere. This design concept introduces a new perspective to develop materials with unique TADF performance and various novel delayed fluorescence-based applications.
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http://dx.doi.org/10.1126/sciadv.1603171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446214PMC
May 2017

Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-β/catenin signaling pathway.

Stem Cell Res Ther 2017 04 26;8(1):98. Epub 2017 Apr 26.

Department of General Surgery, Chinese PLA General Hospital, Beijing, 100853, China.

Background: Ring finger protein 43 (RNF43) is a member of the transmembrane E3 ubiquitin ligase family that was originally found in stem cells and plays important roles in tumor formation and progression. Our previous study indicated that RNF43 might be a tumor suppressor protein in gastric cancer. Given its antagonistic relationship with leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5), one of the gastric cancer stem cell markers, investigation of the potential role of RNF43 in gastric stem cancer cells is necessary.

Methods: Immunohistochemistry staining, western blot analysis, and quantitative reverse transcription polymerase chain reaction were used to determine the mRNA and protein expression level of RNF43 and other Wnt pathway factors. Gastric cancer stem-like cells were obtained from gastric cancer tumor and cell lines by tumorsphere culture. The adeno-associated virus system was used to upregulate RNF43 expression in cancer cells. Functional experiments including tumorsphere formation, chemotherapy resistance, surface marker detection, and tumor xenograft assay were performed to measure stem-like properties in gastric cancer stem-like cells after RNF43 overexpression.

Results: RNF43 loss was significantly associated with TNM stage, distant metastasis, and Lauren classification, and predicted worse prognosis in gastric cancer patients. RNF43 expression was even lower in tumorspheres derived from tumor tissues or cell lines compared with adherent cancer cells and normal gastric cells. Overexpression of RNF43 in gastric cancer cells impaired their stem-like properties, including sphere formation ability, chemoresistance in vitro, and tumorigenicity in vivo. Moreover, Wnt pathway-related proteins were decreased in RNF43-overexpressing cells, while Wnt pathway activators could reverse the trend to some extent.

Conclusions: Our findings indicated that RNF43 might not only participate in gastric cancer progression, but also attenuate the stemness of gastric cancer stem-like cells through the Wnt/β-catenin pathway.
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http://dx.doi.org/10.1186/s13287-017-0548-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5406878PMC
April 2017

Peniproline A, a new 1-phenylamino-2-pyrrolidone metabolite from the endophytic fungus Penicillium decumbens CP-4.

Nat Prod Res 2017 Aug 16;31(15):1772-1777. Epub 2017 Feb 16.

a Department of Biosynthetic Medicinal Chemistry, School of Pharmacy , Fudan University , Shanghai , PR China.

One new (named peniproline A, 1) and 11 known compounds were isolated from a culture of the endophytic fungus Penicillium decumbens CP-4. The structure of 1, featuring a phenylamine unit coupled with 2-pyrrolidone by forming a distinctive nitrogen-nitrogen bond, was elucidated based on extensive spectroscopic methods. Compound 1 was found to have cytotoxic effects against Bel-7402 and Hela cell lines with IC values of 8.1 and 15.5 μM, respectively.
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http://dx.doi.org/10.1080/14786419.2017.1290623DOI Listing
August 2017

Human apolipoprotein A-I exerts a prophylactic effect on high-fat diet-induced atherosclerosis via inflammation inhibition in a rabbit model.

Acta Biochim Biophys Sin (Shanghai) 2017 Feb;49(2):149-158

Department of Biosynthesis & Key Laboratory of Smart Drug Delivery, Ministry of Education, School of Pharmacy, Fudan University, Shanghai 201203, China.

Apolipoprotein A-I (apoA-I) is the major functional protein fraction of high-density lipoprotein. The prophylactic effect and mechanism of human apoA-I on atherosclerosis (AS) were investigated in a high-fat diet-induced AS rabbit model. The rabbits were injected with apoA-I once a week while fed high-fat diet for 20 weeks. Our results showed that apoA-I could raise the serum level of high-density lipoprotein-cholesterol and reduce those of lipid total cholesterol, triglyceride, and low-density lipoprotein-cholesterol in AS rabbits. Decreased aortic plaque area and aortic injury degree were also observed by Oil Red O staining and HE staining in apoA-I-treated high-fat diet-induced AS rabbits. Further study elucidated that apoA-I could down-regulate the expression of some inflammatory mediators including intercellular adhesion molecule type 1, vascular adhesion molecule-1 (VCAM-1), monocyte chemoattractant protein-1, tumor necrosis factor-α, interleukin-6 (IL-6), and C-reactive protein in serum and aorta of AS rabbits. In addition, real-time quantitative RT-PCR analyses showed that the apoA-I infusions decreased the mRNA levels of two pro-inflammatory molecules, i.e. nuclear factor kappa B (NF-κB) and cyclooxygenase-2 (COX-2), in aorta of AS rabbits, which was associated with a concomitant reduction in endothelial VCAM-1 and IL-6 mRNA transcription. Together, our results support the atheroprotective and prophylactic role of apoA-I in vivo, and this activity may be correlated with its anti-inflammatory effect.
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http://dx.doi.org/10.1093/abbs/gmw128DOI Listing
February 2017

Genome-Wide lncRNA Microarray Profiling Identifies Novel Circulating lncRNAs for Detection of Gastric Cancer.

Theranostics 2017 1;7(1):213-227. Epub 2017 Jan 1.

Department of General Surgery & Institute of General Surgery, Chinese People's Liberation Army General Hospital, Fuxing Road 28, Beijing 100853, P.R. China.

Long non-coding RNAs (lncRNAs) can serve as blood-based biomarkers for cancer detection. To identify novel lncRNA biomarkers for gastric cancer (GC), we conducted, for the first time, genome-wide lncRNA screening analysis in two sets of samples: five paired preoperative and postoperative day 14 plasma samples from GC patients, and tissue samples from tumor and adjacent normal tissues. Candidate tumor-related lncRNAs were then quantitated and evaluated in three independent phases comprising 321 participants. The expression levels of lncRNAs were also measured in GC cell lines and the corresponding culture medium. Biomarker panels, lncRNA-based Index I and carcinoembryonic antigen (CEA)-based Index II, were constructed using logistic regression, and their diagnostic performance compared. Fagan's nomogram was plotted to facilitate clinical application. As a result, we identified five novel plasma lncRNAs (, , , and ), which, when combined in the lncRNA-based Index I, outperformed the CEA-based Index II ( < 0.001) and could distinguish GC patients from healthy controls with an area under the receiver-operating curve (AUC) of 0.91 (95% confidence interval (CI): 0.88-0.95). The lncRNA-based index decreased significantly by postoperative day 14 ( = 0.016), indicating its ability to monitor tumor dynamics. High values of the lncRNA-based index were correlated with tumor size ( = 0.036), depth of invasion ( = 0.025), lymphatic metastasis ( = 0.012) and more advanced tumor stages ( = 0.003). The lncRNA-based index was also able to discriminate GC patients from precancerous individuals and patients with gastrointestinal stromal tumor with AUC values of 0.82 (95% CI: 0.71-0.92) and 0.80 (95% CI: 0.68-0.91), respectively. Taken together, our findings demonstrate that this panel of five plasma lncRNAs could serve as a set of novel diagnostic biomarkers for GC detection.
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http://dx.doi.org/10.7150/thno.16044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5196898PMC
October 2017

Diagnostic and prognostic value of circulating tumor DNA in gastric cancer: a meta-analysis.

Oncotarget 2017 Jan;8(4):6330-6340

Chinese PLA General Hospital, Department of General Surgery, Beijing, 100853, People's Republic of China.

Background: Circulating tumor DNA (ctDNA) has offered a minimally invasive approach for detection and measurement of gastric cancer (GC). However, its diagnostic and prognostic value in gastric cancer still remains unclear.

Results: A total of 16 studies comprising 1193 GC patients met our inclusion criteria. The pooled sensitivity and specificity were 0.62 (95% confidence intervals (CI) 0.59-0.65) and 0.95 (95% CI 0.93-0.96), respectively. The AUSROC (area under SROC) curve was 0.94 (95% CI 0.89-0.98). The results showed that the presence of certain ctDNA markers was associated with larger tumor size (OR: 0.26, 95% CI 0.11-0.61, p = 0.002), TNM stage (I + II/III + IV, OR: 0.11, 95% CI 0.07-0.17, p = 0.000), as well as H. pylori infection. (H.p negative/H.p positive, OR: 0.57, 95% CI 0.36-0.91, p = 0.018). Moreover, there was also a significant association between the presence of ctDNA and worse overall survival (HR 1.77, 95% CI 1.38-2.28, p < 0.001), as well as disease-free survival (HR 4.36, 95% CI 3.08-6.16, p < 0.001).

Materials And Methods: Pubmed, Embase, Cochrane Library and Web of Science databases were searched for relating literature published up until November 30, 2016. Diagnostic accuracy variables were pooled by the Meta-Disc software. Engauge Digitizer and Stata software were applied for prognostic data extraction and analysis.

Conclusions: Our meta-analysis indicates the detection of certain ctDNA targets is significantly associated with poor prognosis of GC patients, with high specificity and relatively moderate sensitivity.
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http://dx.doi.org/10.18632/oncotarget.14064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351635PMC
January 2017

C2-ceramide enhances sorafenib-induced caspase-dependent apoptosis via PI3K/AKT/mTOR and Erk signaling pathways in HCC cells.

Appl Microbiol Biotechnol 2017 Feb 2;101(4):1535-1546. Epub 2016 Nov 2.

Department of Biosynthesis and Key Laboratory of Smart Drug Delivery, Ministry of Education, School of Pharmacy, Fudan University, Shanghai, China.

Sorafenib as an effective multikinase inhibitor has been approved for the clinical treatment against advanced hepatocellular carcinoma (HCC). HCC treatment requires usually combined therapy because of its complex pathogenesis. Ceramide has been confirmed to induce remarkable apoptosis in human tumor cells and has attracted increasing attention in investigations on combination therapy. In this paper, the anti-HCC effect of sorafenib combined with C2-ceramide was investigated on cell vitality, apoptosis, and migration, and the underlying mechanism was examined using flow cytometry and western blot. Bel7402 cells coincubated with sorafenib and C2-ceramide exhibited lower cell vitality and more irregular cellular morphology and cell cycle arrest. Sorafenib plus C2-ceramide stimulated significantly the production of reactive oxygen species (ROS) and mitochondrial depolarization, which promoted caspases-dependent cell apoptosis as illustrated by related protein expression including caspase 3, caspase 9, Bax, Bcl-2, and cytochrome c. Combination treatment of sorafenib and C2-ceramide inhibited obviously cell growth and proliferation via PI3K/AKT/mTOR and Erk signaling pathways. Furthermore, the combination treatment was proved to inhibit cell migration and epithelial-mesenchymal transition (EMT). These findings indicated that the combination of C2-ceramide and sorafenib provided synergistic inhibitory effects on HCC cells.
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http://dx.doi.org/10.1007/s00253-016-7930-9DOI Listing
February 2017

Chlorogenic acid prevents isoproterenol-induced DNA damage in vascular smooth muscle cells.

Mol Med Rep 2016 Nov 15;14(5):4063-4068. Epub 2016 Sep 15.

Department of Obstetrics and Gynecology, Tongji Hospital of Tongji University, Tongji University School of Medicine, Shanghai 200065, P.R. China.

Numerous clinical therapeutic agents have been identified as DNA damaging. The present study revealed that isoproterenol (Iso) resulted in DNA damage in vascular smooth muscle cells (VSMCs) and increased the levels of intracellular oxygen free radicals. Administration of chlorogenic acid (CGA) inhibited this effect. Pretreatment with CGA abrogated the increase in protein expression levels of γ‑H2A histone family member X, phosphorylated ataxia telangiectasia mutated, phosphorylated Rad3‑related protein, breast cancer 1 and C‑terminal Src homologous kinase induced by Iso. In addition, the increase in levels of intracellular reactive oxygen species (ROS) induced by Iso was inhibited by CGA pretreatment in a dose‑dependent manner. The results of the present study suggest that CGA may inhibit Iso‑induced VSMC damage via the suppression of ROS generation. Therefore, CGA may be a novel agent for the treatment of vascular diseases.
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http://dx.doi.org/10.3892/mmr.2016.5743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101879PMC
November 2016

The Activity of Fungichromin against the Formation of Candida albicans Biofilm.

Biol Pharm Bull 2016 Dec 8;39(12):1948-1954. Epub 2016 Sep 8.

Department of Biosynthetic Medicinal Chemistry, School of Pharmacy, Fudan University.

The effect of fungichromin (FC) on the formation of Candida albicans biofilm was assessed using 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction method, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Results showed that FC revealed an inhibitory effect on the formation of C. albicans biofilm in a dose-dependent manner with a minimum inhibitory concentration (MIC) of 10 µg/mL. Over 80% of biofilm formation was inhibited by FC at the concentration of 40 µg/mL when compared with the control. Similarly, real-time PCR showed that the expression of the genes such as ALS1, ALS3, HWP1, EFG1, HYR1, CPH1 and BCR1 appeared to be remarkably affected by FC at the concentration of 20 µg/mL during the biofilm formation. In addition, FC could also induce the apoptosis of C. albicans cells in a dose-dependent manner. Therefore, FC displayed potent activity against the formation of C. albicans biofilm in vitro and played an important role in reducing the incidence of device-associated infections.
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http://dx.doi.org/10.1248/bpb.b16-00380DOI Listing
December 2016