Publications by authors named "Jiong Zhou"

105 Publications

A physician survey of poststroke aphasia diagnosis and treatment in China: SPEECH study.

Medicine (Baltimore) 2021 Jun;100(22):e25833

Drug Development Organization, Lundbeck China, Beijing, China.

Abstract: The current status of the diagnosis and management of poststroke aphasia (PSA) in China is unknown.To analyze the physicians' strategy and knowledge about the management of PSA in clinical practice and the needs for standardization of diagnosis and treatment.This survey was conducted in March-August 2019 at 32 tertiary hospitals in 16 provinces/municipalities in China. The attending physicians from the Neurology and Neuro-rehabilitation/Rehabilitation Departments were included. The online questionnaire inquired about patient information, physicians' diagnosis and treatment behavior for PSA, and physicians' understanding of PSA.A total of 236 physicians completed the survey. Regarding PSA assessment, 99.2% of the physicians reported using medical history and physical examination, 93.2% reported using neuroimaging, and 76.3% reported using dedicated scales. Most physicians used a combination of drug and non-drug treatment. Neuro-regenerators/cerebral activators and anti-dementia drugs were the most common pharmacotherapies; butylphthalide, edaravone, and memantine were most frequently prescribed. Six months poststroke was rendered as a spontaneous language recovery period, and a ≥6-month treatment for PSA was suggested by many physicians. The lack of standardized treatment regimen/clinical guidelines and the limited number of approved drugs for PSA were the primary challenges encountered by physicians during practice. The majority of the physicians agreed with the necessity of guidelines or consensus for the diagnosis and treatment of PSA.The knowledge gaps exist among physicians in China regarding the assessment and management of PSA. The improved awareness of the available guidelines/consensus could improve the performance of the physicians.
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http://dx.doi.org/10.1097/MD.0000000000025833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183701PMC
June 2021

Simultaneous nitrogen and phosphorus removal from simulated digested piggery wastewater in a single-stage biofilm process coupling anammox and intracellular carbon metabolism.

Bioresour Technol 2021 Aug 17;333:125152. Epub 2021 Apr 17.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China.

A Single-stage biofilm process coupling Anammox and Intracellular Carbon metabolism (SAIC) was constructed for treating simulated digested piggery wastewater with low carbon/nitrogen ratio (C/N) in this study. TN removal in SAIC system increased by more than 12.77% compared to the reference, and the maximum total phosphorus (TP) removal efficiency reached to 83.70% (C/N = 1.5). Denitrification driven by intracellular carbon, mainly poly-β-hydroxybutyrate (PHB, 78.57%), contributed 32.60% of TN elimination at most, and at least 67.40% should be attributed to anammox. Phosphorus was thought to be mainly removed through biological route, while chemical precipitation also explained around 10% of removed TP. Furthermore, commensalism of glycogen accumulating organisms (GAOs), phosphate accumulating organisms (PAOs), nitrifiers and anammox bacteria was revealed by combining 16S rRNA amplicon sequencing and metagenomics. As a result, multiple metabolic pathways including anammox, (partial) nitrification, endogenous (partial) denitrification and biological P-removal played synergistic effect in SAIC system.
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http://dx.doi.org/10.1016/j.biortech.2021.125152DOI Listing
August 2021

Distinct impaired patterns of intrinsic functional network centrality in patients with early- and late-onset Alzheimer's disease.

Brain Imaging Behav 2021 Apr 12. Epub 2021 Apr 12.

Department of Radiology, The 2nd Affiliated Hospital of Zhejiang University School of Medicine, No.88 Jie-fang Road, Shang-cheng District, Hangzhou, 310009, China.

Early-onset Alzheimer's disease (EOAD) involves multiple cognitive domains and shows more rapid progression than late-onset Alzheimer's disease (LOAD). However, the difference in pathogenesis between EOAD and LOAD is still unclear. Accordingly, we applied intrinsic network analysis to explore the potential neuropathological mechanism underlying distinct clinical phenotypes. According to the cut-off age of 65, we included 20 EOAD patients, 20 LOAD patients, and 36 age-matched controls (19 young and 17 old controls). We employed resting-state functional MRI and network centrality analysis to explore the local (degree centrality (DC)) and global (eigenvector centrality (EC)) functional integrity. Two-sample t-test analysis was performed, with gray matter volume, age, gender, and education as covariates. Furthermore, we performed a correlation analysis between network metrics and cognition. Compared to young controls, EOAD patients exhibited lower DC in the middle temporal gyrus (MTG), parahippocampal gyrus (PHG), superior temporal gyrus (STG), and lower EC in the MTG, PHG, and postcentral gyrus. In contrast, LOAD patients exhibited lower DC in the STG and anterior cingulum gyrus and higher DC in the middle frontal gyrus compared to old controls. No significant difference in EC was observed in LOAD patients. Furthermore, both DC and EC correlated with cognitive performance. Our study demonstrated divergent functional network impairments in EOAD and LOAD patients. EOAD patients showed more complex network damage involving both local and global centrality properties, while LOAD patients mainly featured local functional connectivity changes. Such centrality impairments are related to poor cognition, especially regarding memory performance.
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http://dx.doi.org/10.1007/s11682-021-00470-3DOI Listing
April 2021

Supramolecular cancer nanotheranostics.

Chem Soc Rev 2021 Feb 1;50(4):2839-2891. Epub 2021 Feb 1.

State Key Laboratory of Chemical Engineering, Center for Chemistry of High-Performance & Novel Materials, Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China.

Among the many challenges in medicine, the treatment and cure of cancer remains an outstanding goal given the complexity and diversity of the disease. Nanotheranostics, the integration of therapy and diagnosis in nanoformulations, is the next generation of personalized medicine to meet the challenges in precise cancer diagnosis, rational management and effective therapy, aiming to significantly increase the survival rate and improve the life quality of cancer patients. Different from most conventional platforms with unsatisfactory theranostic capabilities, supramolecular cancer nanotheranostics have unparalleled advantages in early-stage diagnosis and personal therapy, showing promising potential in clinical translations and applications. In this review, we summarize the progress of supramolecular cancer nanotheranostics and provide guidance for designing new targeted supramolecular theranostic agents. Based on extensive state-of-the-art research, our review will provide the existing and new researchers a foundation from which to advance supramolecular cancer nanotheranostics and promote translationally clinical applications.
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http://dx.doi.org/10.1039/d0cs00011fDOI Listing
February 2021

Vapochromic Behaviors of A Solid-State Supramolecular Polymer Based on Exo-Wall Complexation of Perethylated Pillar[5]arene with 1,2,4,5-Tetracyanobenzene.

Angew Chem Int Ed Engl 2021 Apr 3;60(15):8115-8120. Epub 2021 Mar 3.

State Key Laboratory of Chemical Engineering, Key Laboratory of Excited-State Materials of Zhejiang Province, Department of Chemistry, Zhejiang University, Hangzhou, 310027, P. R. China.

The research for the solid-state supramolecular polymers with specific functions accelerates the development of supramolecular and materials sciences. Herein, we discover the different complexation modes of perethylated pillar[5]arene (EtP5) with 1,2,4,5-tetracyanobenzene (TCNB) in various solvents. Driven by charge-transfer interaction, TCNB is enclosed in the cavity of EtP5 in CHCl , while TCNB complexes with EtP5 at the exo-wall of EtP5 in CH Cl . This is because the size of CH Cl matches the cavity of EtP5, forcing TCNB to complex with the exo-wall of EtP5. Furthermore, we fabricate a vapochromic solid-state supramolecular polymer by exploiting the exo-wall complexation, which turns from brown to reddish brown or black after adsorption of alkyl aldehyde vapors. The adsorptive nature for alkyl aldehyde vapors comes from the unoccupied cavity of EtP5 based on C-H⋅⋅⋅π interactions. The vapochromic property is attributed to the change of the charge-transfer interaction caused by molecular rearrangement induced by vapor-capture in the solid-state supramolecular polymer.
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http://dx.doi.org/10.1002/anie.202013701DOI Listing
April 2021

Hydraulic flow direction alters nutrients removal performance and microbial mechanisms in electrolysis-assisted constructed wetlands.

Bioresour Technol 2021 Apr 11;325:124692. Epub 2021 Jan 11.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.

In this study, an electrolysis-assisted down-flow constructed wetland (E-DFCW) was successfully established, and achieved simultaneously efficient removal of PO-P (93.6% ± 3.2%), NO-N (97.1% ± 2.0%) and TN (80.6% ± 5.4%). When compared with electrolysis-assisted up-flow constructed wetland (E-UFCW), E-DFCW allowed significantly lower concentrations of PO-P, NO-N, total Fe and SO-S in effluents. In addition, microbial community and functional genes prediction results indicated that hydraulic flow direction significantly altered microbial nitrogen, sulfur and carbon metabolisms in electrolysis-assisted constructed wetlands (E-CWs). Specifically, multi-path denitrification facilitated NO-N reduction in cathodic chamber of E-DFCW, whereas autohydrogenotrophic denitrification might dominate NO-N reduction in cathodic chamber of E-UFCW. More abundant and diverse denitrifiers in cathodic chamber of E-DFCW contributed to enhanced denitrification performance. Overall, this work provides microbial insights into multi-path nitrogen metabolisms in electrolysis-assisted denitrification systems in response to hydraulic flow direction.
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http://dx.doi.org/10.1016/j.biortech.2021.124692DOI Listing
April 2021

Inhibits Growth and Migration of Lung Cancer Cells through Regulating p53-Bcl2 and MMPs Pathways.

Am J Chin Med 2020 9;48(8):1941-1953. Epub 2020 Dec 9.

School of Life Sciences, University of Technology Sydney, NSW 2007, Australia.

has been shown to possess antitumor activity. This study investigated the effects and mechanisms of extract (ACE) on growth and migration of human non-small cell lung cancer A549 cells. The effect of ACE on cell viability was determined by MTT assay and fluorescent live-cell imaging. The apoptotic effect of ACE was determined by cell cycle distribution using flow cytometry. A P53-mediated apoptosis pathway was identified by measuring protein expression of p53 and Bcl-2 with Western blotting. Additionally, mRNA expression of p53 and -2 and was detected by qRT-PCR. The effect of ACE on cancer cell migration was confirmed by a wound-healing assay. Expression of MMP-2 and MMP-9 at the protein and gene levels was determined by western blot and qRT-PCR analysis. This study demonstrates the inhibitory effect of ACE on A549 cell proliferation in a dose-response manner with an [Formula: see text]. It was determined that ACE concentration at [Formula: see text] induced cell cycle arrest at S phase in A549 cells. The apoptosis-regulating protein p53 expression was enhanced and also associated with the downregulation of Bcl-2 in ACE treatment cells. The mRNA expression of and associated with was consistent with protein expression. The inhibition of migration of cancer cells treated with ACE was clearly evident. At the same time, suppression of expression of MMP-2 and MMP-9 at protein and mRNA levels was observed. The findings of this study highlight ACE as a potential agent of adjuvant therapy for lung cancer.
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http://dx.doi.org/10.1142/S0192415X20500974DOI Listing
May 2021

Distinct Atrophy Pattern of Hippocampal Subfields in Patients with Progressive and Stable Mild Cognitive Impairment: A Longitudinal MRI Study.

J Alzheimers Dis 2021 ;79(1):237-247

Department of Radiology, 2nd Affiliated Hospital of Zhejiang University School of Medicine, China.

Background: Predicting the prognosis of mild cognitive impairment (MCI) has outstanding clinical value, and the hippocampal volume is a reliable imaging biomarker of AD diagnosis.

Objective: We aimed to longitudinally assess hippocampal sub-regional difference (volume and asymmetry) among progressive MCI (pMCI), stable MCI (sMCI) patients, and normal elderly.

Methods: We identified 29 pMCI, 52 sMCI, and 102 normal controls (NC) from the ADNI database. All participants underwent neuropsychological assessment and 3T MRI scans three times. The time interval between consecutive MRI sessions was about 1 year. Volumes of hippocampal subfield were measured by Freesurfer. Based on the analysis of variance, repeated measures analyses, and receiver operating characteristic curves, we compared cross-sectional and longitudinal alteration sub-regional volume and asymmetry index.

Results: Compared to NC, both MCI groups showed significant atrophy in all subfields. At baseline, pMCI have a smaller volume than sMCI in the bilateral subiculum, molecular layer (ML), the molecular and granule cell layers of the dentate gyrus, cornu ammonis 4, and right tail. Furthermore, repeated measures analyses revealed that pMCI patients showed a faster volume loss than sMCI in bilateral subiculum and ML. After controlling for age, gender, and education, most results remained unchanged. However, none of the hippocampal sub-regional volumes performed better than the whole hippocampus in ROC analyses, and no asymmetric difference between pMCI and sMCI was found.

Conclusion: The faster volume loss in subiculum and ML suggest a higher risk of disease progression in MCI patients. The hippocampal asymmetry may have smaller value in predicting the MCI prognosis.
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http://dx.doi.org/10.3233/JAD-200775DOI Listing
January 2021

The Draft Genome of Red Lechwe, .

Front Genet 2020 5;11:582638. Epub 2020 Nov 5.

School of Ecology and Environment, Northwestern Polytechnical University, Xi'an, China.

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http://dx.doi.org/10.3389/fgene.2020.582638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7674670PMC
November 2020

Selective Separation of Methylfuran and Dimethylfuran by Nonporous Adaptive Crystals of Pillararenes.

J Am Chem Soc 2020 11 9;142(46):19722-19730. Epub 2020 Nov 9.

State Key Laboratory of Chemical Engineering, Center for Chemistry of High-Performance & Novel Materials, Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China.

The separation of 2-methylfuran () and 2,5-dimethylfuran () mixtures is very important in the chemical industry. Herein, we offer a novel strategy for the separation of and using nonporous adaptive crystals (NACs) of perethylated pillar[5]arene (), perethylated pillar[6]arene (), perbromoethylated pillar[5]arene (), and perbromoethylated pillar[6]arene (). We find that the crystals of and show remarkable selectivities for in a 50:50 (/) : mixture vapor, yielding purities of 94.0 and 96.3%, respectively. Single-crystal structures reveal that these different selectivities come from the different thermodynamic stabilities and binding modes of the host-guest complexes. Cycling experiments demonstrate that these crystals can be reused more than five cycles without loss of performance.
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http://dx.doi.org/10.1021/jacs.0c09757DOI Listing
November 2020

Epidemiology, mortality and risk factors for patients with K. pneumoniae bloodstream infections: Clinical impact of carbapenem resistance in a tertiary university teaching hospital of Beijing.

J Infect Public Health 2020 Nov 17;13(11):1710-1714. Epub 2020 Oct 17.

Department of Medical Affairs, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China. Electronic address:

Background: This study compared the epidemiology of carbapenem-resistant (CRKP) and carbapenem-sensitive (CSKP) K. pneumoniae bloodstream infections (BSIs), and assessed risk factors for 28-day mortality of patients with K. pneumoniae BSIs.

Methods: A retrospective cohort study was conducted in a 2000-bed tertiary teaching hospital of Beijing between Jan 1st 2013 to Dec 31st, 2019. All patients with K. pneumoniae BSI were identified through the Hospital Information System. The endpoints included incidence rate, mortality and risk factors for mortality of patients with K. pneumoniae BSIs.

Results: 496 patients with K. pneumoniae BSIs were included in the analysis, with 108 CRKP BSIs. The incidence rate of K. pneumoniae BSI was 10.6 (CI: 9.7, 11.6) per 100 000 patient-days, with the rate for CRKP BSI was 2.3 (95% CI: 1.9, 2.8). The 28-day mortality was 38.0% for CRKP BSI and 8.8% for CSKP BSI, respectively. Logistic analysis showed, higher Charlson Comorbidity Index score (OR = 1.26, 95%CI 1.12-1.43, p < 0.001), respiratory failure (OR = 2.73, 95%CI1.28-5.84, p = 0.010), renal failure (OR = 4.13, 95%CI1.93-8.83, p < 0.001), septic shock (OR = 8.77, 95%CI3.60-21.32, p < 0.001), mechanical ventilation (OR = 4.41, 95%CI1.59-12.25, p = 0.004) and CRKP infection (OR = 3.04, 95%CI1.28-7.22, p = 0.012) were independently associated with 28-day mortality.

Conclusions: Considerable incidence rate and remarkable mortality of patients with K. pneumoniae (especially CRKP) BSI was declared in the study. Patient conditions before (higher CCI) and after presentation (respiratory failure, renal failure, septic shock), and healthcare factors (mechanical ventilation and CRKP infection) were independently associated with 28-day mortality. Understanding these risks helps better establishment of infection control strategies.
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http://dx.doi.org/10.1016/j.jiph.2020.09.012DOI Listing
November 2020

Facilitating harmful algae removal in fresh water via joint effects of multi-species algicidal bacteria.

J Hazard Mater 2021 02 12;403:123662. Epub 2020 Aug 12.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China. Electronic address:

Harmful algae blooms posing serious threats to the ecological environment occur frequently across the world. Multi-species algicidal bacteria were enriched by utilizing immobilized carriers in a pilot scale experiment, which significantly promoted the effect of algal control in the reactors. Under the optimal condition, the algicidal ratio and chlorophyll a degradation rate reached 87.69% and 47.00 μg/(L·d), respectively. The growth of Cyanophyta, diatom, Dinoflagellate and Cryptophyta was inhibited significantly by the joint action of algicidal bacteria and light shading of fillers, accounting for 53.74% and 36.47%, respectively. The results of 16S rRNA high-throughput sequencing suggested algicidal bacteria (10.17%) belonging to 13 genera were enriched. Among the algicidal process, Bacillus and Pseudomonas played crucial roles. Fluorescence spectroscopy and UV were adopted to assess the release of dissolved organic matter (DOM) and the precursors of disinfection by-products (DBPs). Two efficient algicidal strains (C1, C4) were isolated which showed high homology with Enterobacter asburiae JCM6051(T) and Pseudomonas simiae oli(T), respectively. This study provided new insights into the in-situ bioremediation of eutrophication in fresh water.
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http://dx.doi.org/10.1016/j.jhazmat.2020.123662DOI Listing
February 2021

The variation on nitrogen removal mechanisms and the succession of ammonia oxidizing archaea and ammonia oxidizing bacteria with temperature in biofilm reactors treating saline wastewater.

Bioresour Technol 2020 Oct 1;314:123760. Epub 2020 Jul 1.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China. Electronic address:

To reveal nitrogen removal mechanisms under environmental stresses, biofilm reactors were operated at different temperatures (10 °C-35 °C) treating saline wastewater (salinity 3%). The results showed nitrogen removal efficiency was 98.46% at 30 °C and 60.85% at 10 °C, respectively. Both ammonia oxidizing archaea (AOA) and ammonia oxidizing bacteria (AOB) participated in nitrification. 94.9% of the overall ammonia oxidation was attributed to AOA at 10 °C, but only 48.2% of that was undertaken by AOA at 35 °C. AOA had a greater contribution at low temperature, which demonstrated that nitrogen removal pathway varied with temperature. Aerobic denitrification was more stable than anoxic denitrification. High-throughput sequencing showed Crenarchaeota was the dominant AOA (97.02-34.47%), cooperating with various heterotrophic AOB. Real-time PCR indicated that AOA was three orders of magnitude more abundant than AOB. AOA was more resistant to low temperature and high-saline stresses. Ammonia oxidizers had distinct responses to temperature change and showed diverse relationships at different temperatures.
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http://dx.doi.org/10.1016/j.biortech.2020.123760DOI Listing
October 2020

Progressive Memory Circuit Impairments along with Alzheimer's Disease Neuropathology Spread: Evidence from in vivo Neuroimaging.

Cereb Cortex 2020 10;30(11):5863-5873

Department of Radiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.

During the progression of Alzheimer's disease (AD), neuropathology may propagate transneuronally, cause disruption in memory circuit, and lead to memory impairment. However, there is a lack of in vivo evidence regarding this process. Thus, we aim to simulate and observe the progression of neuropathology in AD continuum. We included cognitively normal (CN), mild cognitive impairments (MCI), and AD subjects, and further classified them using the A/T/N scheme (Group 0: CN, A - T-; Group 1: CN, A + T-; Group 2: CN, A + T+; Group 3: MCI, A + T+; Group 4: AD, A + T+). We investigated alterations of three core memory circuit structures: hippocampus (HP) subfields volume, cingulum-angular bundles (CAB) fiber integrity, and precuneus cortex volume. HP subfields volume showed the trend of initially increased and then decreased (starting from Group 2), while precuneus volume decreased in Groups 3 and 4. The CAB integrity degenerated in Groups 3 and 4 and aggravated with higher disease stages. Further, memory circuit impairments were correlated with neuropathology biomarkers and memory performance. Conclusively, our results demonstrated a pattern of memory circuit impairments along with AD progression: starting from the HP, then propagating to the downstream projection fiber tract and cortex. These findings support the tau propagation theory to some extent.
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http://dx.doi.org/10.1093/cercor/bhaa162DOI Listing
October 2020

Green tea polyphenols ameliorate metabolic abnormalities and insulin resistance by enhancing insulin signalling in skeletal muscle of Zucker fatty rats.

Clin Sci (Lond) 2020 05;134(10):1167-1180

School of Life Sciences, University of Technology Sydney, NSW 2007, Australia.

In the present study, we evaluated the metabolic effects of green tea polyphenols (GTPs) in high-fat diet (HFD) fed Zucker fatty (ZF) rats, in particular the effects of GTP on skeletal muscle insulin sensitivity. Body weight, visceral fat, glucose tolerance, lipid profiles and whole-body insulin sensitivity were measured in HFD-fed ZF rats after 8-week-treatment with GTP (200 mg/kg of body weight) or saline (5 ml/kg of body weight). Zucker lean rats were studied as controls. Ex vivo insulin-mediated muscle glucose uptake was assessed. Immunoblotting was used to evaluate the expression of key insulin signalling proteins in skeletal muscle. GTP treatment attenuated weight gain (P<0.05) and visceral fat accumulation (27.6%, P<0.05), and significantly reduced fasting serum glucose (P<0.05) and insulin (P<0.01) levels. Homoeostasis model assessment of insulin resistance (HOMA-IR), a measure of insulin resistance, was lower (P<0.01) in GTP-treated animals compared with ZF controls. Moreover, insulin-stimulated glucose uptake by isolated soleus muscle was increased (P<0.05) in GTP-ZF rats compared with ZF-controls. GTP treatment attenuated the accumulation of ectopic lipids (triacyl- and diacyl-glycerols), enhanced the expression and translocation of glucose transporter-4, and decreased pSer612IRS-1 and increased pSer473Akt2 expression in skeletal muscle. These molecular changes were also associated with significantly decreased activation of the inhibitory (muscle-specific) protein kinase (PKC) isoform, PKC-θ. Taken together, the present study has shown that regular ingestion of GTP exerts a number of favourable metabolic and molecular effects in an established animal model of obesity and insulin resistance. The benefits of GTP are mediated in part by inhibiting PKC-θ and improving muscle insulin sensitivity.
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http://dx.doi.org/10.1042/CS20200107DOI Listing
May 2020

Influence of eugenol on algal growth, cell physiology of cyanobacteria Microcystis aeruginosa and its interaction with signaling molecules.

Chemosphere 2020 Sep 30;255:126935. Epub 2020 Apr 30.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, PR China. Electronic address:

Essential oils (EOs) are naturally occurring substances that have shown great prospect in the field of antimicrobial, antioxidant and pest control by nontoxic mechanisms. In this regard, EOs are considered the promising and eco-friendly approach for controlling harmful algae. In this study, the anti-cyanobacterial activity of EOs eugenol against Microcystis aeruginosa are evaluated from the perspective of photosynthetic efficiency, the behavior of extracellular organic matter (EOM), endogenous plant hormone synthesis, and nitric oxide signaling pathway. Results showed that the photosynthetic activity of M. aeruginosa decreased significantly after eugenol treatments. Eugenol treatment resulted in cells rupture and the release of EOM. Levels of endogenous plant hormones salicylic acid (SA) and jasmonic acid (JA) were enhanced separately by 2.32 and 2.01 times after 4 d of exposure to eugenol. And the inhibition of SA and JA biosynthesis further promotes the inhibitory effects of eugenol on algae. Additionally, the signaling molecule nitric oxide (NO) increased significantly by 3.78-fold. Furthermore, the influence of NO on microalgae exposed to eugenol was also determined, suggesting that the inhibitory effect of eugenol stress might be associated with NO generation in M. aeruginosa. These findings will be helpful for the understanding of the fate and potential of eugenol in harmful algae control.
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http://dx.doi.org/10.1016/j.chemosphere.2020.126935DOI Listing
September 2020

Distinct Spontaneous Brain Activity Patterns in Different Biologically-Defined Alzheimer's Disease Cognitive Stage: A Preliminary Study.

Front Aging Neurosci 2019 17;11:350. Epub 2019 Dec 17.

Department of Radiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.

: The National Institute on Aging-Alzheimer's Association (NIA-AA) has proposed a biological definition of Alzheimer's disease (AD): individuals with both abnormal amyloid and tau biomarkers (A+T+) would be defined as AD. It remains unclear why different cognitive status is present in subjects with biological AD. Resting-state functional magnetic resonance imaging (rsfMRI) has provided an opportunity to reveal the brain activity patterns in a biologically-defined AD cohort. Accordingly, we aimed to investigate distinct brain activity patterns in subjects with existed AD pathology but in the different cognitive stages. : We selected individuals with AD pathology (A+T+) and healthy controls (HC, A-T-) based on the cerebrospinal fluid (CSF) biomarkers. According to the cognitive stage, we divided the A+T+ cohort into three groups: (1) preclinical AD; (2) prodromal AD; and (3) AD with dementia (d-AD). We compared spontaneous brain activity measured by a fractional amplitude of low-frequency fluctuation (fALFF) approach among four groups. : The analysis of covariance (ANCOVA) results showed significant differences in fALFF in the posterior cingulate cortex/precuneus (PCC/PCu). Further, compared to HC, we found increased fALFF values in the right inferior frontal gyrus (IFG) in the preclinical AD stage, whereas prodromal AD patients showed reduced fALFF in the bilateral precuneus, right middle frontal gyrus (MFG), right precentral gyrus, and postcentral gyrus. Within the d-AD group, both hyperactivity (right fusiform gyrus, right parahippocampal gyrus (PHG)/hippocampus, and inferior temporal gyrus) and hypoactivity (bilateral precuneus, left posterior cingulate cortex, left cuneus and superior occipital gyrus) were detected. : We found the distinct brain activity patterns in different cognitive stages among the subjects defined as AD biologically. Our findings may be helpful in understanding mechanisms leading to cognitive changes in the AD pathophysiological process.
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http://dx.doi.org/10.3389/fnagi.2019.00350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6980867PMC
December 2019

Separation of Benzene and Cyclohexane by Nonporous Adaptive Crystals of a Hybrid[3]arene.

J Am Chem Soc 2020 02 27;142(5):2228-2232. Epub 2020 Jan 27.

State Key Laboratory of Chemical Engineering, Center for Chemistry of High-Performance & Novel Materials, Department of Chemistry , Zhejiang University , Hangzhou 310027 , People's Republic of China.

The separation of benzene and cyclohexane is one of the most challenging tasks in the petrochemical field. However, conventional separation methods suffer from cumbersome operation, huge energy expenditure, or use of entrainers. Herein, we develop an environmentally friendly and energy saving adsorptive separation strategy using nonporous adaptive crystals of a hybrid[3]arene (). Adaptive crystals separate benzene from an equimolar benzene/cyclohexane mixture with a purity of 97.5%. The selectivity comes from the stability and variability of the new crystal structure upon capture of the preferred guest, benzene. Moreover, reversible transformations between the nonporous guest-free structure and the guest-containing structure make highly recyclable.
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http://dx.doi.org/10.1021/jacs.9b13548DOI Listing
February 2020

Effects of COD/TN ratio on nitrogen removal efficiency, microbial community for high saline wastewater treatment based on heterotrophic nitrification-aerobic denitrification process.

Bioresour Technol 2020 Apr 3;301:122726. Epub 2020 Jan 3.

Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China.

High salinity and high organic concentration impose negative impacts on autotrophic nitrification, which hinders efficient nitrogen removal. To achieve efficient nitrogen removal, high saline wastewater nitrogen removal systems with different COD/TN ratios based on heterotrophic nitrification-aerobic denitrification (HN-AD) process were established in this study. Results demonstrated that the COD/TN ratio had significant effects on nitrogen removal efficiency, microbial community structures and metabolic pathways. The optimal COD/TN ratio was 25 for nitrogen removal, with NH-N and TN removal rates of 11.86 mg·L·h and 11.50 mg·L·h, respectively (3.65 and 3.31 times higher than those COD/TN ratio of 10). 16S rRNA high-throughput sequencing revealed that HN-AD functional bacteria (Pseudomonas, Bacillus, Paracoccus) with highest abundance (7.61%) played a key role in high saline wastewater treatment. And Halomonas, Nitrincola and Oceanimonas participated in the denitrification process. Moreover, the abundance of genes related to nitrogen removal was the highest (1.90%) revealed by functional genes prediction.
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http://dx.doi.org/10.1016/j.biortech.2019.122726DOI Listing
April 2020

Indirect Regulation and Equilibrium of p35 and p40 Subunits of Interleukin (IL)-12/23 by Ustekinumab in Psoriasis Treatment.

Med Sci Monit 2020 Jan 7;26:e920371. Epub 2020 Jan 7.

Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China (mainland).

BACKGROUND Ustekinumab, a human-derived monoclonal antibody that targets the p40 subunit of interleukin (IL)-12 and IL-23, has excellent clinical efficacy and safety in treating psoriasis, with a long half-life. However, no reports have described the use of human skin/serum samples to elucidate its molecular mechanisms. MATERIAL AND METHODS Twenty-four psoriasis patients were enrolled in our double-blind study and randomly divided into placebo and ustekinumab-administered groups. Dynamic changes in psoriasis area-severity index scores, and mRNA and protein levels of p35 and p40 were analyzed at 3 time points (before treatment and during the 12th and 24th weeks of treatment). RESULTS Ustekinumab initially increased and then decreased p35 mRNA expression, but increased p40 mRNA levels throughout the study. The p35 protein levels were not significantly altered, while p40 protein levels were increased after the first 2 injections but decreased after the third injection. CONCLUSIONS We concluded that 2 equilibria influence the efficacy of ustekinumab against psoriasis. First, because of the dual roles of p35 in psoriasis pathogenesis, homeostasis occurs between p35 and p40 expression levels. The second balance lies between the upregulation of p40 mRNA levels and the ability of ustekinumab to neutralize the function of the elevated p40 protein.
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http://dx.doi.org/10.12659/MSM.920371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977627PMC
January 2020

Interactions between sleep disturbances and Alzheimer's disease on brain function: a preliminary study combining the static and dynamic functional MRI.

Sci Rep 2019 12 13;9(1):19064. Epub 2019 Dec 13.

Department of Radiology, 2nd Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang, China.

Though sleep disturbance constitutes the risk factor for Alzheimer's disease (AD), the underlying mechanism is still unclear. This study aims to explore the interaction between sleep disturbances and AD on brain function. We included 192 normal controls, 111 mild cognitive impairment (MCI), and 30 AD patients, with either poor or normal sleep (PS, NS, respectively). To explore the strength and stability of brain activity, we used static amplitude of low-frequency fluctuation (sALFF) and dynamic ALFF (dALFF) variance. Further, we examined white matter hyperintensities (WMH) and amyloid PET deposition, representing the vascular risk factor and AD-related hallmark, respectively. We observed that sleep disturbance significantly interacted with disease severity, exposing distinct effects on sALFF and dALFF variance. Interestingly, PS groups showed the dALFF variance trajectory of initially increased, then decreased and finally increased along the AD spectrum, while showing the opposite trajectory of sALFF. Further correlation analysis showed that the WMH burden correlates with dALFF variance in PS groups. Conclusively, our study suggested that sleep disturbance interacts with AD severity, expressing as effects of compensatory in MCI and de-compensatory in AD, respectively. Further, vascular impairment might act as important pathogenesis underlying the interaction effect between sleep and AD.
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http://dx.doi.org/10.1038/s41598-019-55452-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911090PMC
December 2019

Expert Consensus on the Care and Management of Patients with Cognitive Impairment in China.

Neurosci Bull 2020 Mar 2;36(3):307-320. Epub 2019 Dec 2.

HuanHu Hospital of Nankai University, Tianjin, 300350, China.

The cognitive disease consensus was prepared by panels of health and public representatives based on actual clinical practice in Geriatric Departments in Chinese hospitals and a systematic literature review. This consensus reflects the medical knowledge accumulated by those experts and provides information about professional medical care and advice. A multidisciplinary panel of specialists (neurologists, psychiatrists, and nursing specialists) reports an expert consensus on the medical knowledge accumulated from those experts and provides information about professional medical care and advice. The recommendations focus on the care and management of older adults with mild cognitive impairment, the objectives and methods of maintaining cognition and training, the assessments and measures of daily care for patients at different stages of dementia, the assessments and coping strategies for the behavioral and psychological symptoms of dementia, principles and suggestions for an appropriate living environment, arrangements for recreational activities, the care and management of patients with end-stage dementia, and suggestions for addressing stress in caregivers.
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http://dx.doi.org/10.1007/s12264-019-00444-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056776PMC
March 2020

Path analysis from physical activity to quality of life among dementia patients: A dual-path mediating model.

J Adv Nurs 2020 Feb 25;76(2):546-554. Epub 2019 Nov 25.

Xuanwu Hospital Capital Medical University, Beijing, China.

Aims: To clarify the specific impact paths among physical activity, activity of daily living, depression and quality of life among dementia patients.

Design: A descriptive, cross-sectional design.

Methods: Dementia patients and their caregivers from five tertiary general hospitals and one dementia patients' club were recruited. A total of 216 valid questionnaires were collected from November 2018 - March 2019. Path analysis was performed by Mplus 7.0 to test the casual relationship among physical activity, activity of daily living, depression and quality of life.

Results: Most patients with dementia presented a low level of physical activity and suffered from impaired quality of life. The modified model presented a good model fit and revealed that physical activity had indirect positive effects on quality of life mediated by activity of daily living and depression.

Conclusion: The results showed that physical activity was a promising non-pharmacological method to improve the performance of activity of daily living and reduce depressive symptoms and then enhance the quality of life among dementia patients.

Impact: The findings were beneficial to elevate the awareness of physical activity among patients and professionals. This study was helpful to understand how physical activity exerted effects on life quality of dementia patients. This study provided a new perspective for researchers to elucidate the causal relationship of variables among dementia patients.
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http://dx.doi.org/10.1111/jan.14260DOI Listing
February 2020

Separation of Monochlorotoluene Isomers by Nonporous Adaptive Crystals of Perethylated Pillar[5]arene and Pillar[6]arene.

J Am Chem Soc 2019 10 18;141(43):17102-17106. Epub 2019 Oct 18.

State Key Laboratory of Chemical Engineering, Center for Chemistry of High-Performance & Novel Materials, Department of Chemistry , Zhejiang University , Hangzhou 310027 , P. R. China.

Separation of monochlorotoluene isomers is a vital process to obtain highly pure -chlorotoluene, which is irreplaceable in the production of medicines and pesticides. However, traditional separation methods suffer from great energy consumption, cumbersome operation or use of organic desorbents. Herein, an energy-efficient and environmentally friendly method is developed through an absorptive separation strategy based on nonporous adaptive crystals of perethylated pillar[5]arene () and pillar[6]arene (). and crystals separate -chlorotoluene from a -chlorotoluene/-chlorotoluene equimolar mixture with purities of 99.1% and 96.1%, respectively and show no decrease in selectivity upon cycling. The selectivity is attributed to both the stability of the final crystal structure upon guest capture and suitable host cavity size/shape. Besides, we discovered the gate-opening behavior changes of crystals at different temperatures after absorption of -chlorotoluene/-chlorotoluene mixtures with various -chlorotoluene fractions, which is helpful to understand the thermodynamics of the absorption process.
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http://dx.doi.org/10.1021/jacs.9b09988DOI Listing
October 2019

Application of T1-/T2-Weighted Ratio Mapping to Elucidate Intracortical Demyelination Process in the Alzheimer's Disease Continuum.

Front Neurosci 2019 10;13:904. Epub 2019 Sep 10.

Department of Radiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

The biological diagnosis criteria of the Alzheimer's disease (AD) suggests that previous work may misclassify the cognitive impairment caused by other factors into AD. Consequently, re-assessing the imaging profile of AD continuum is needed. Considering the high vulnerability of cortical association fibers, we aimed to elucidate the cortical demyelination process in the AD continuum biologically defined. According to the biological diagnosis criteria, we determined the positive amyloid status (A+) as cerebrospinal fluid (CSF) amyloid < 192 pg/ml, Florbetapir Positron emission tomography (PET) composite standardized uptake value ratio (SUVR) >1.11. Also, the positive Tau status (T+) was determined as p-Tau > 23 pg/ml. Based on the cognitive characterization, we further categorized 252 subjects into 27 cognitively unimpaired with normal AD biomarkers (A-T-, controls), 49 preclinical AD (A+T+), 113 AD with mild cognitive impairment (MCI) (A+T+), and 63 AD dementia (A+T+). We estimated the intracortical myelin content used the T1- and T2-weighted (T1W/T2W) ratio mapping. To investigate the sensitivity of the ratio mapping, we also utilized well-validated AD imaging biomarkers as the reference, including gray matter volume and Fludeoxyglucose PET (FDG-PET). Based on the general linear model, we conducted the voxel-wise two-sample -tests between the controls and each group in the AD continuum. Compared to the controls, the results showed that the preclinical AD patients exhibited decreased T1W/T2W ratio value in the right inferior parietal lobule (IPL); as the disease progresses, the prodromal AD patients demonstrated lower ratio value in bilateral IPL, with hippocampus (HP) atrophy. Lastly, the AD dementia patients exhibited decreased ratio value in bilateral IPL and hippocampus; also, we observed the bilateral temporal cortices atrophy and widespread decreased metabolism in the AD dementia patients. After corrected with gray volume, the results remained mostly unchanged. Our study implied the decreased right IPL T1W/T2W ratio might represent early AD-related demyelination in disease continuum. Additionally, we demonstrated that the T1W/T2W ratio mapping is an easy-to-implement and sensitive metric to assess the intracortical myelin content in AD, particularly in the early stage.
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http://dx.doi.org/10.3389/fnins.2019.00904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748350PMC
September 2019

[2]Pseudorotaxane-Based Supramolecular Optical Indicator for the Visual Detection of Cellular Cyanide Excretion.

Chemistry 2019 Nov 16;25(63):14447-14453. Epub 2019 Oct 16.

State Key Laboratory of Chemical Engineering, Center for Chemistry of High-Performance & Novel Materials, Department of Chemistry, Zhejiang University, Hangzhou, 310027, P. R. China.

Cyanide is extremely hazardous to living organisms and the environment. Owing to its wide range of applications and high toxicity, the development of functional materials for cyanide detection and sensing is highly desirable. Host-guest complexation between bis(p-phenylene)-34-crown-10 H and N-methylacridinium salt G remarkably decreases the detection limit for cyanide anions compared with that of the guest itself. The [2]pseudorotaxane selectively recognizes the cyanide anion with high optical sensitivity as a result of the nucleophilic addition of the cyanide anion at the 9-position of G. The host-guest complexation is further incorporated into supramolecular materials for the visual detection of cyanide anions, especially the detection of cellular cyanide excretion with a detection limit of 0.6 μm. This supramolecular method provides an extremely distinct strategy for the visual detection of cyanide anions.
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http://dx.doi.org/10.1002/chem.201903577DOI Listing
November 2019

Nanomedicine Fabricated from A Boron-dipyrromethene (BODIPY)-Embedded Amphiphilic Copolymer for Photothermal-Enhanced Chemotherapy.

ACS Biomater Sci Eng 2019 Sep 23;5(9):4463-4473. Epub 2019 Aug 23.

Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland 20892, United States.

To overcome the shortcomings of chemotherapy including side effects and uncontrollable release, as well as to increase the therapeutic efficacy, a diblock copolymer (mPEG--PLA-BODIPY) was constructed containing a NIR absorbing boron-dipyrromethene (BODIPY) tail, a hydrophobic polylactide (PLA) segment, and a hydrophilic poly(ethylene glycol) (PEG) segment. The nanoparticles self-assembled from mPEG--PLA-BODIPY with a core-shell structure were utilized to load docetaxel (DTX) in the core through hydrophobic interaction. Tailored drug release and high tumor penetration of the nanomedicine were realized by fully taking advantage of photothermal effect and the enhanced penetration and retention effect, facilitating enhanced therapeutic performance and reducing undesirable side effects. antitumor studies demonstrate that photothermal-enhanced chemotherapy effectively suppresses tumor progression, while systemic toxicity and side effects of DTX are remarkably decreased benefiting from rational design. This pioneering example provides a blueprint for the next generation of polymeric delivery vehicles integrating novel theranostic functions.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01145DOI Listing
September 2019

Pharmacokinetics and Safety of Icotinib Hydrochloride Cream in Patients with Mild to Moderate Chronic Plaque Psoriasis: A Randomized Double-Blind Vehicle-Controlled Phase 1 Study.

Biomed Res Int 2019 2;2019:9072683. Epub 2019 May 2.

Center of Clinical Pharmacology, The Second Affiliated Hospital, Zhejiang University School of Medicine, China.

Objective: This phase I study aimed to systematically assess the safety, local tolerability, pharmacokinetics, and preliminary efficacy of topical icotinib hydrochloride cream in patients with mild to moderate plaque psoriasis.

Materials And Methods: Eligible Chinese adult patients with mild to moderate psoriasis were assigned to the icotinib cream or vehicle group. Icotinib cream with increasing concentrations (0.5%, 1.0%, 2.0%, and 4.0%) or vehicle were administered by the fingertip unit method to the skin lesions twice a day for 4 weeks. Safety assessments included the incidence and severity of adverse events (AEs), local tolerability at the treatment area, vital signs, and laboratory examinations. Plasma levels of icotinib were also measured for the pharmacokinetics calculation. The efficacy was preliminarily explored by assessing the improvement in the severity level using Target Plaque Severity Score (TPSS) and overall improvement using the Psoriasis Area Severity Index (PASI) and Dermatological Quality Life Index.

Results: Forty-one patients were enrolled and qualified for safety analysis. 27 (65.9%) patients experienced at least one AE, of which application-site adverse drug reactions (ADRs) were reported in 6 (14.6%) patients. All ADRs were of grade 1 or 2, most common irritation (4.5%), itching (3.1%), and erythema (2.4%), and resolved during follow-up. The systemic exposure to icotinib was very low; the highest plasma concentration was 0.214 ng/mL, while the area under the curve from 0 to 12 hours was 1.626 h·ng/mL. The TPSS improved for all icotinib groups after treatment in a dose- and time-dependent manner.

Conclusion: This phase 1 study demonstrated favorable safety, tolerable toxicity, and preliminary efficacy of icotinib cream in patients with mild to moderate psoriasis. The dose concentration of 2.0% (twice daily based on the fingertip unit method) is recommended for further study.

Study Design: This is a single-center, randomized, double-blind, and vehicle-controlled study.
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http://dx.doi.org/10.1155/2019/9072683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6521334PMC
December 2019

Self-Assembled Amphiphilic Janus Double Metallacycle.

Inorg Chem 2019 Jun 16;58(11):7141-7145. Epub 2019 May 16.

Department of Chemistry , University of Utah , 315 South 1400 East, Room 2020 , Salt Lake City , Utah 84112 , United States.

A double metallacycle was prepared via the size-selective integrative self-sorting of four different building blocks driven by a reversible metal-ligand coordination interaction. A hydrophobic dendron was placed on a metallacycle and a hydrophilic dendron was attached to the other metallacycle, producing a two-faced Janus-type supramolecule with two distinct functionalities. In aqueous media, hierarchical self-assembly of the supramolecular system was induced by the combination of coordination interactions and hydrophobic-hydrophilic interactions resulting in the formation of micrometer-sized fiber-like structures, a morphology distinct from metallacycles bearing only one type of functionality. This study provides a versatile approach for the construction of Janus-type molecules and demonstrates that integrative self-sorting of a supramolecular coordination system can be utilized for the preparation of complex supramolecular systems with predesigned functionalities and morphologies.
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http://dx.doi.org/10.1021/acs.inorgchem.9b01036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690363PMC
June 2019

Discovery of non-classical complex models between a cationic water-soluble pillar[6]arene and naphthalenesulfonate derivatives and their self-assembling behaviors.

Soft Matter 2019 May;15(20):4127-4131

Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China.

Molecular recognition based on cationic water-soluble pillar[n]arenes shows considerable advantages in their application in biological and environmental systems, such as excellent anion-binding ability and antimicrobial properties. Unique complex models are discovered in this work where a cationic water-soluble pillar[6]arene binds with disodium 1,5-naphthalenedisulfonate and disodium 2,6-naphthalenedisulfonate at the ratio of 1 : 2, which is proven by results from nuclear magnetic resonance spectroscopy, isothermal titration calorimetry, fluorescence spectroscopy experiments and transmission electron microscopy.
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http://dx.doi.org/10.1039/c9sm00659aDOI Listing
May 2019
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