Publications by authors named "Jintao Wang"

131 Publications

Synthesis of ruthenium complexes functionalized with benzothiophene and their antibacterial activity against Staphylococcus aureus.

Dalton Trans 2021 Apr 9;50(16):5607-5616. Epub 2021 Apr 9.

School of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang, 330013, China.

New effective antimicrobial agents with novel modes of action are urgently needed due to the continued emergence of drug-resistant bacteria. Here, three ruthenium complexes functionalized with benzothiophene: [Ru(phen)(BTPIP)](ClO) (Ru(II)-1), [Ru(dmp)(BTPIP)](ClO) (Ru(II)-2) and [Ru(dmb)(BTPIP)](ClO) (Ru(II)-3) (dmb = 4,4'-dimethyl-2,2'-bipyridine, phen = 1,10-phenanthroline, dmp = 2,9-dimethyl-1,10-phenanthroline) have been synthesized and their antimicrobial activities in vitro were assessed. Minimum inhibitory concentration (MIC) assays indicated that the three Ru(II)-1, Ru(II)-2 and Ru(II)-3 complexes all showed antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. The most active Ru(II)-3 complex was further tested against biofilms. Furthermore, it was also tested whether complex Ru(II)-3 could serve as an antibacterial adjuvant. Interestingly, the checkerboard data showed that Ru(II)-3 selectively exhibited synergism with aminoglycoside antibiotics. More importantly, the observed synergetic effect might be attributed to the inhibition of the regulatory function of SaCcpA. Finally, in vivo bacterial infection treatment studies through a murine skin infection model and skin irritation test were also conducted. All in all, these results confirmed that ruthenium complexes functionalized with benzothiophene have good antimicrobial activity against Staphylococcus aureus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0dt04258gDOI Listing
April 2021

Efficient combination of Human Papillomavirus Genotyping for the triage of women with Atypical Squamous Cells of Undetermined Significance in Chinese rural population: A population-based study.

J Cancer 2021 14;12(10):2815-2824. Epub 2021 Mar 14.

Department of Obstetrics and Gynecology, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China.

In this prospective, population-based study, we evaluated the utility of high-risk human papillomavirus (HR-HPV) genotyping for triaging women with atypical squamous cells of undetermined significance (ASC-US) in the Chinese rural area. A total of 40,000 women were recruited from rural areas of Shanxi Province, China, between June 2014 and December 2014. Women with Pap results of ASC-US underwent HPV genotyping, colposcopy and histopathological examination. For those with normal cervixes or cervical intraepithelial neoplasia (CIN) 1 on the initial evaluation, a 2-year follow-up study was performed. The reporting rate of ASC-US was 5.76% (2,304/40,000) in the study population. The detection rates of CIN 2 or above (CIN2+) and CIN 3 or above (CIN3+) in women with ASC-US were 7.28% and 1.75%, respectively. HPV 16 (39.53%), HPV 58 (17.83%), and HPV 52 (15.50%) were the three most prevalent HR-HPV genotypes among all women with ASC-US cytology. The five most common HR-HPV genotypes in CIN3+ lesions were HPV16, HPV58, HPV33, HPV31 and HPV18. Compared with the 15 HR-HPV testing, genotyping for a combination of HPV16/18/31/33/58 increased specificity significantly with virtually no loss of sensitivity for detecting CIN2+ and CIN3+ lesions, as well as significantly reduced colposcopy referral rate (23.15% vs 33.70%, p<0.01). In addition, in the 2-year follow-up period, women with infection of HPV16, 18, 31, 33 or 58 genotypes were the most likely population (92%, 23/25) to develop CIN2 lesion. Our results demonstrate that genotyping for a combination of HPV16/18/31/33/58 provides a more efficient and cost-effective model to risk-stratify women with ASC-US in the Chinese rural population.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.55771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040879PMC
March 2021

LINC00221 silencing prevents the progression of hepatocellular carcinoma through let-7a-5p-targeted inhibition of MMP11.

Cancer Cell Int 2021 Apr 9;21(1):202. Epub 2021 Apr 9.

Department of Oncology, Shaanxi Province, Affiliated Hospital of the Shaanxi University of Traditional Chinese Medicine, No. 2, Weiyang West Road, Xianyang, 712000, People's Republic of China.

Background: Microarray profiles of hepatocellular carcinoma (HCC) identified that long intergenic noncoding RNA 00221 (LINC00221) was upregulated. Herein, we aimed to identify the functional significance and underlying mechanisms of LINC00221 in HCC.

Methods And Results: Human HCC samples had increased expression of LINC00221. Effects of LINC00221 on HCC cellular functions were analyzed using gain- and loss-function approaches. LINC00221 knockdown repressed HCC cell growth, migration, and invasion and enhanced their apoptosis. This anti-tumor effect was validated in vivo. Online prediction showed the potential binding relationship between LINC00221 and let-7a-5p, as well as that between let-7a-5p and matrix metalloproteinase 11 (MMP11). The results of luciferase, RNA immunoprecipitation, and RNA pull-down assays identified that LINC00221 interacted with let-7a-5p to increase expression of MMP11. Furthermore, we demonstrated that LINC00221 silencing increased let-7a-5p and inhibited MMP11 expression, thereby delaying the progression of HCC in vitro.

Conclusions: Silencing of LINC00221 could prevent HCC progression via upregulating let-7a-5p and downregulating MMP11. As such, LINC00221 inhibition presents a promising antitumor strategy for the treatment of HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12935-021-01819-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035785PMC
April 2021

Diagnosis and gI antibody dynamics of pseudorabies virus in an intensive pig farm in Hei Longjiang Province.

J Vet Sci 2021 Mar;22(2):e23

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Gaoxin District, Daqing, Hei Longjiang 163319, China.

Background: Pseudorabies (PR), caused by the pseudorabies virus (PRV), is an endemic disease in some regions of China. Although there are many reports on epidemiological investigations into pseudorabies, information on PRV gI antibody dynamics in one pig farm is sparse.

Objectives: To diagnose PR and analyze the course of PR eradication in one pig farm.

Methods: Ten brains and 1,513 serum samples from different groups of pigs in a pig farm were collected to detect PRV gE gene and PRV gI antibody presence using real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.

Results: The July 2015 results indicated that almost all brain samples were PRV gE gene positive, but PRV gI antibody results in the serum samples of the same piglets were all negative. In the boar herd, from October 2015 to July 2018 three positive individuals were culled in October 2015, and the negative status of the remaining boars was maintained in the following tests. In the sow herd, the PRV gI antibody positive rate was always more than 70% from October 2015 to October 2017; however, it decreased to 27% in January 2018 but increased to 40% and 52% in April and July 2018, respectively. The PRV gI antibody positive rate in 100-day pigs markedly decreased in October 2016 and was maintained at less than 30% in the following tests. For 150-day pigs, the PRV gI antibody positive rate decreased notably to 10% in April 2017 and maintained a negative status from July 2017. The positive trend of PRV gI antibody with an increase in pig age remarkably decreased in three tests in 2018.

Conclusions: The results indicate that serological testing is not sensitive in the early stage of a PRV infection and that gilt introduction is a risk factor for a PRV-negative pig farm. The data on PRV gI antibody dynamics can provide reference information for pig farms wanting to eradicate PR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4142/jvs.2021.22.e23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007445PMC
March 2021

Photooxidation crosslinking to recover residual stress in decellularized blood vessel.

Regen Biomater 2021 Mar 13;8(2):rbaa058. Epub 2021 Mar 13.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400030, China.

Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular grafts. However, this method will reduce the opening angle of the blood vessel and result in the reduction of residual stress. Residual stress reduced has an adverse effect on the compliance and permeability of small diameter vascular grafts. To improve the situation, acellular blood vessels were treated with glutaraldehyde and photooxidation crosslinking respectively, and the changes of opening angle, circumferential residual strain of native blood vessels, decellularized arteries and crosslinked blood vessels were measured by means of histological examination, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) in this study. The opening angle of decellularized arteries significantly restored after photooxidation crosslinking ( = 0.0216), while that of glutaraldehyde crosslinking blood vessels reduced. The elastic fibers inside the blood vessels became densely rearranged after photooxidation crosslinking. The results of finite element simulation showed that the residual stress increased with the increase of opening angle. In this study, we found at the first time that photooxidation crosslinking method could significantly increase the residual stress of decellularized vessels, which provides biomechanical support for the development of new biomaterials of vascular grafts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/rb/rbaa058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955719PMC
March 2021

Endothelial cell-activating antibodies in COVID-19.

medRxiv 2021 Jan 20. Epub 2021 Jan 20.

Patients with coronavirus disease 19 ( ) are at high risk for fibrin-based occlusion of vascular beds of all sizes. Although endothelial cell activation has regularly been described as part of the COVID-19 thrombo-inflammatory storm, the upstream mediators of this activation have yet to be fully elucidated. Here, we began by pursuing the hypothesis that circulating factors such as neutrophil extracellular trap (NET) remnants, D-dimer, or C-reactive protein might predict the COVID-19 serum samples (n=118) that most robustly activated cultured endothelial cells. Indeed, we found modest correlations between serum NET remnants (cell-free DNA, myeloperoxidase-DNA complexes, citrullinated histone H3) and upregulation of surface E-selectin, VCAM-1, and ICAM-1 on endothelial cells. However, a more robust predictor of the ability of COVID-19 serum to activate endothelial cells was the presence of circulating antiphospholipid antibodies, specifically anticardiolipin IgG and IgM and anti-phosphatidlyserine/prothrombin (anti-PS/PT) IgG and IgM. Depletion of total IgG from anticardiolipin-high and anti-PS/PT-high samples markedly restrained upregulation of E-selectin, VCAM-1, and ICAM-1. At the same time, supplementation of control serum with patient IgG was sufficient to trigger endothelial cell activation. These data are the first to reveal that patient antibodies are a driver of endothelial cell activation and add important context regarding thrombo-inflammatory effects of COVID-19 autoantibodies in severe COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1101/2021.01.18.21250041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836141PMC
January 2021

Optimizing Age Penalty in Time-Varying Networks with Markovian and Error-Prone Channel State.

Entropy (Basel) 2021 Jan 10;23(1). Epub 2021 Jan 10.

Department of Electronic Engineering, Tsinghua University, Beijing 100084, China.

In this paper, we consider a scenario where the base station (BS) collects time-sensitive data from multiple sensors through time-varying and error-prone channels. We characterize the data freshness at the terminal end through a class of monotone increasing functions related to Age of information (AoI). Our goal is to design an optimal policy to minimize the average age penalty of all sensors in infinite horizon under bandwidth and power constraint. By formulating the scheduling problem into a constrained Markov decision process (CMDP), we reveal the threshold structure for the optimal policy and approximate the optimal decision by solving a truncated linear programming (LP). Finally, a bandwidth-truncated policy is proposed to satisfy both power and bandwidth constraint. Through theoretical analysis and numerical simulations, we prove the proposed policy is asymptotic optimal in the large sensor regime.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/e23010091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827969PMC
January 2021

Lower dietary mineral intake is significantly associated with cervical cancer risk in a population-based cross-sectional study.

J Cancer 2021 1;12(1):111-123. Epub 2021 Jan 1.

Department of Obstetrics and Gynecology, Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Population-based studies investigating the association between dietary mineral intake and risk of cervical intraepithelial neoplasia (CIN) or cervical cancer in Chinese women are few. We performed a cross-sectional analysis of screening data obtained from 2,304 women in 2014 within an ongoing cohort study comprising 40,000 women in China. Dietary intake was assessed using a semiquantitative food frequency questionnaire. Nutrition intake was calculated using a 26-item list of food sources drawn from a validated, comprehensive database. All participants were surveyed through in-person interviews, physical examinations, and laboratory tests. The Pearson chi-square test was used for categorical variables. Multivariable logistic regression models were used to evaluate the relationship between dietary mineral intake and CIN+ risk. The food frequency questionnaire exhibited acceptable reproducibility and reasonable validity in assessing nutrient intakes among these women. After adjusting for multiple potential confounders, low dietary calcium intake was associated with CIN2+ risk (first versus fourth quartile: odds ratio [OR]=1.52, 95% confidence interval [CI]: 1.01-2.32). Similar for magnesium (OR=1.80, 95% CI: 1.20-2.68), phosphorus (OR=1.69, 95% CI: 1.12-2.55), zinc (OR=1.55, 95% CI: 1.03-2.34), and potassium (OR=1.92, 95% CI: 1.28-2.88). Low dietary intakes of calcium and potassium were significantly associated with CIN1 risk. Increased CIN2+ risk correlated with rates of no oral contraceptives and lower levels of dietary Potassium. These results thus proposed that low dietary mineral intake was an independent risk factor, potential synergy may exist between low dietary mineral levels and oral contraceptives contribute to the development of higher-grade CIN and cervical cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.39806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738836PMC
January 2021

Prevalence and Genotype Distribution of High-Risk Human Papillomavirus Infection in Women with Abnormal Cervical Cytology: A Population-Based Study in Shanxi Province, China.

Cancer Manag Res 2020 8;12:12583-12591. Epub 2020 Dec 8.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030000, People's Republic of China.

Purpose: High-risk human papillomavirus (HR-HPV) infection is widely known as the major cause of cervical cancer and there are notable differences in HR-HPV prevalence and genotype distribution in different populations. Women with abnormal cervical cytology are at increased risk of cervical cancer; however, the genotype distribution of HR-HPV in women with abnormal cervical cytology remains unclear.

Methods: A total of 2,300 women with abnormal cervical cytology (from 39,988 women completing a baseline survey in a cohort established during June 2014 to December 2014) were enrolled in this study. All participants gave informed consent and completed a questionnaire about characteristics related to HPV infection. HPV genotypes were identified using flow-through hybridization, and cytology was assessed by the ThinPrep cytological test. Data were analyzed using SPSS 22.0 for Windows.

Results: The overall prevalence of HR-HPV in the 2,300 women with abnormal cervical cytology was 32%, with single and multiple HR-HPV infections making up 70.2% and 29.8%, respectively. The top-five HR-HPV genotypes were HPV16 (13.5%), HPV58 (5.7%), HPV52 (4.9%), HPV53 (2.5%), and HPV51 (2.3%). The prevalence of HR-HPV in atypical squamous cells of undetermined significance, low-grade squamous intraepithelial lesions, and high-grade squamous intraepithelial lesions or higher was 30.8%, 36.5%, and 54.9%, respectively, showing an increasing trend with severity of cervical cytology (=13.952, <0.001). The prevalence of HPV16 and HPV33 increased significantly with the degree of cytological abnormality. HR-HPV infection risk was statistically higher in women aged 35-45 years, with low education, infrequent bathing, multiple gravidity, multiple parity, history of gynecological diseases, and premenopause.

Conclusion: HR-HPV infection in women with abnormal cervical cytology was 32%, and the top-five HR-HPV genotypes were HPV16, HPV58, HPV52, HPV53, and HPV51 in Shanxi Province, China. These results shed light on demographic and behavioral characteristics related to HR-HPV infection in women with abnormal cervical cytology and provide an insight for the development of HPV vaccines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/CMAR.S269050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733379PMC
December 2020

Dietary nutrient intake related to higher grade cervical intraepithelial neoplasia risk: a Chinese population-based study.

Nutr Metab (Lond) 2020 Nov 30;17(1):100. Epub 2020 Nov 30.

Department of Obstetrics and Gynecology, Second Hospital of Shanxi Medical University, 382 Wuyi Rd, Taiyuan, 030001, Shanxi, China.

Background: Dietary nutrient intake plays a significant role in carcinogenesis. Few studies have investigated the association between dietary nutrient intake and cervical intraepithelial neoplasia (CIN) risk in China.

Methods: Data on 2304 women from an ongoing cohort comprising 40,000 women from China in 2014 were included. Study randomly selected 218 out of 2304 people as subjects during 2019. All participants were surveyed through in-person interviews, physical examinations, and laboratory tests. Clinical data were obtained from physical examinations and laboratory tests. Dietary intakes were assessed using a semiquantitative food frequency questionnaire. Nutrition intakes from 26 food sources were calculated using a comprehensive validated database. Descriptive statistics were used to describe the frequency and proportion, and mean and standard deviation of the demographic characteristics. Characteristics were examined for significant differences, and Pearson chi-square tests were used for categoric variables. Logistic regression was used to obtain odds ratios (ORs) and confidence intervals (CIs) for CIN risk in each nutrient intake quartile relative to that in the highest quartile.

Results: The food frequency questionnaire exhibited acceptable reproducibility and reasonable validity in assessing nutrient intakes among these women. After adjusting for multiple confounders, several dietary nutrients showed significant associations with CIN2+ risk. Low dietary folate intake was associated with the risk of CIN2+ (first versus fourth quartile: OR = 1.55, 95% CI 1.03-2.33). Similar results were also observed for vitamin B6 (OR = 1.63, 95% CI 1.08-2.46), vitamin C (OR = 1.59, 95% CI 1.05-2.42), niacin (OR = 1.65, 95% CI 1.08-2.51), and vitamin K (second versus fourth quartile: OR = 1.60, 95% CI 1.05-2.44).

Conclusions: Low folate; vitamin B6, C, and K; and niacin intakes were associated with CIN2+ risk. Nutrients may influence the development of higher grade CIN and cervical cancer. Trial registration The study was registered in the Chinese Clinical Trial Register (ChiCTR-ROC-15006479) ( https://www.chictr.org.cn ).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12986-020-00521-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708219PMC
November 2020

Prothrombotic autoantibodies in serum from patients hospitalized with COVID-19.

Sci Transl Med 2020 11 2;12(570). Epub 2020 Nov 2.

Division of Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.

Patients with COVID-19 are at high risk for thrombotic arterial and venous occlusions. Lung histopathology often reveals fibrin-based blockages in the small blood vessels of patients who succumb to the disease. Antiphospholipid syndrome is an acquired and potentially life-threatening thrombophilia in which patients develop pathogenic autoantibodies targeting phospholipids and phospholipid-binding proteins (aPL antibodies). Case series have recently detected aPL antibodies in patients with COVID-19. Here, we measured eight types of aPL antibodies in serum samples from 172 patients hospitalized with COVID-19. These aPL antibodies included anticardiolipin IgG, IgM, and IgA; anti-β glycoprotein I IgG, IgM, and IgA; and anti-phosphatidylserine/prothrombin (aPS/PT) IgG and IgM. We detected aPS/PT IgG in 24% of serum samples, anticardiolipin IgM in 23% of samples, and aPS/PT IgM in 18% of samples. Antiphospholipid autoantibodies were present in 52% of serum samples using the manufacturer's threshold and in 30% using a more stringent cutoff (≥40 ELISA-specific units). Higher titers of aPL antibodies were associated with neutrophil hyperactivity, including the release of neutrophil extracellular traps (NETs), higher platelet counts, more severe respiratory disease, and lower clinical estimated glomerular filtration rate. Similar to IgG from patients with antiphospholipid syndrome, IgG fractions isolated from patients with COVID-19 promoted NET release from neutrophils isolated from healthy individuals. Furthermore, injection of IgG purified from COVID-19 patient serum into mice accelerated venous thrombosis in two mouse models. These findings suggest that half of patients hospitalized with COVID-19 become at least transiently positive for aPL antibodies and that these autoantibodies are potentially pathogenic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/scitranslmed.abd3876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724273PMC
November 2020

2.8  µm passively Q-switched Er:ZBLAN fiber laser with an Sb saturable absorber mirror.

Appl Opt 2020 Oct;59(29):9165-9168

A -switched Er:ZBLAN fiber laser operating at 2.8 µm was realized by employing Sb as the saturable material. The Sb material was deposited on a gold mirror by the magnetron-sputtering deposition method to develop a saturable absorber mirror (SAM). By employing the Sb-SAM in an Er:ZBLAN fiber laser, stable -switching operation was achieved at central wavelength of 2799.7 nm with the repetition rates ranging from 33.3 to 58.8 kHz and the pulse duration ranging from 5.7 to 1.7 µs. The Sb-SAM still works stably under the maximum pump power of 5.6 W, with an output power of 59 mW corresponding to the pulse energy of 1.03 µJ. To our knowledge, this was the first demonstration of Sb-based saturable material in Er:ZBLAN fiber laser for mid-infrared -switched pulse generation operating in the 2.8 µm regime, indicating its potential applications in the mid-infrared waveband.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.402227DOI Listing
October 2020

Cadherin repeat 5 mutation associated with Bt resistance in a field-derived strain of pink bollworm.

Sci Rep 2020 10 8;10(1):16840. Epub 2020 Oct 8.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Evolution of resistance by pests reduces the benefits of transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt). Here we analyzed resistance to Bt toxin Cry1Ac in a field-derived strain of pink bollworm (Pectinophora gossypiella), a global pest of cotton. We discovered that the r14 allele of the pink bollworm cadherin gene (PgCad1) has a 234-bp insertion in exon 12 encoding a mutant PgCad1 protein that lacks 36 amino acids in cadherin repeat 5 (CR5). A strain homozygous for this allele had 237-fold resistance to Cry1Ac, 1.8-fold cross-resistance to Cry2Ab, and developed from neonate to adult on Bt cotton producing Cry1Ac. Inheritance of resistance to Cry1Ac was recessive and tightly linked with r14. PgCad1 transcript abundance in midgut tissues did not differ between resistant and susceptible larvae. Toxicity of Cry1Ac to transformed insect cells was lower for cells expressing r14 than for cells expressing wild-type PgCad1. Wild-type PgCad1 was transported to the cell membrane, whereas PgCad1 produced by r14 was not. In larval midgut tissue, PgCad1 protein occurred primarily on the brush border membrane only in susceptible larvae. The results imply r14 mediates pink bollworm resistance to Cry1Ac by reduced translation, increased degradation, and/or mislocalization of cadherin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-74102-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7544870PMC
October 2020

Antibacterial Zwitterionic Polyelectrolyte Hydrogel Adhesives with Adhesion Strength Mediated by Electrostatic Mismatch.

ACS Appl Mater Interfaces 2020 Oct 1;12(41):46816-46826. Epub 2020 Oct 1.

School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou 510275, P. R. China.

Biotissue adhesives and antibacterial materials have great potential applications in wound dressing, implantable devices, and bioelectronics. In this study, stretchable tissue adhesive hydrogels with intrinsic antibacterial properties have been demonstrated by copolymerizing zwitterionic monomers with ionic monomers. The hydrogels are stretchable to about 900% strain and show a modulus of 4-9 kPa. The zwitterionic moieties provide strong dipole-dipole interaction, electrostatic interaction, and hydrogen bonding with the skin surface, and thus show adhesion strength values of 1-4 kPa to skin. Meanwhile, the copolymerized cationic or anionic monomers break the intrinsic electrostatic stoichiometry of the zwitterionic units and thus mediate the electrostatic interactions and the adhesion strength with the surface. The stretchable hydrogels form a robust and compliant (due to low modulus and stretchability) adhesive to skin, rubber, glass, and plastics, and could be repeatedly peeled-off and readhered to the skin. Moreover, the abundant quaternary ammonium (QA) groups in the zwitterionic moieties and the added QA groups endow it outstanding antibacterial properties (>99%). These stretchable tissue adhesive antibacterial hydrogels are promising for wound dressings and implantable devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c14959DOI Listing
October 2020

Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease.

Haematologica 2020 08 13. Epub 2020 Aug 13.

University of Michigan Internal Medicine - Cardiology division, Ann Arbor, MI, USA;

Sickle cell disease (SCD) is associated with chronic hemolytic anemia and a heightened inflammatory state. The causal role of inflammatory pathways in stroke associated with SCD is unclear. Therefore, the hypothesis that deletion of the non-hematopoietic interleukin-1 receptor (IL-1R) pool may be beneficial in SCD was pursued. Since potential deleterious effects of IL-1R signaling in SCD could be mediated via downstream production of interleukin-6 (IL-6), the role of the non-hematopoietic IL-6 pool was also addressed. Bone marrow transplantation (BMT) from SCD to wild-type (WT) recipient mice was used to generate SCD mice (Wt,SCDbmt). To generate mice with non-hematopoietic deficiency of IL-1R or IL-6, SCD marrow was transplanted into IL-1R deficient (IL1R-/-,SCDbmt) or IL-6 deficient recipients (IL6-/-,SCDbmt). Blood counts, reticulocytes, soluble E-selectin (Esel), and IL-6 levels were analyzed 14-15 weeks post-BMT. Ischemic stroke was induced by middle cerebral artery (MCA) photothrombosis at 16 weeks post-BMT. A separate group of Wt,SCDbmt mice was given the IL-1R inhibitor, anakinra, following stroke induction. Seventy-two hours after MCA occlusion, stroke volume was assessed by staining brain sections with 2,3,5-triphenyltetrazolium chloride. Formalin-fixed brain sections were also stained for macrophages with MAC3, for endothelial activation with ICAM-1, and for loss of blood brain barrier (BBB) integrity with fibrin(ogen) staining. All SCD mice generated by BMT were anemic and the severity of anemia was not different between Wt,SCDbmt, IL1R-/-,SCDbmt, and IL6-/-,SCDbmt mice. Three days following MCA occlusion, stroke volume was significantly reduced in IL1R-/-,SCDbmt mice compared to Wt,SCDbmt mice and IL6-/-,SCDbmt mice. Plasma levels of sE-sel were lower in IL1R-/-,SCDbmt compared to Wt,SCDbmt and IL-6-/-,SCDbmt mice. Post-stroke treatment of Wt,SCDbmt mice with anakinra decreased stroke size, leukocyte infiltration, ICAM-1 expression, and fibrin(ogen) accumulation compared to vehicle-treated mice. Deficiency of non-hematopoietic IL-1R or treatment with an IL-1R antagonist is sufficient to confer protection against the increased stroke size associated with SCD. These effects of IL1R deficiency are associated with reduced endothelial activation, leukocyte infiltration, and blood brain barrier disruption, and are independent of non-hematopoietic IL-6 signaling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3324/haematol.2020.252395DOI Listing
August 2020

Magnetic Stress Sensing System for Nondestructive Stress Testing of Structural Steel and Steel Truss Components Based on Existing Magnetism.

Sensors (Basel) 2020 Jul 21;20(14). Epub 2020 Jul 21.

Mechanical Engineering College, Xi'an Shiyou University, Xi'an 710065, China.

To detect the stress of steel structures and members using the existing magnetism, a magnetic stress sensing system integrating a magnetic flux induction coil, a magnetic flux measurement device, a loaded device, and data acquisition software was developed. The magnetic coupling test research was carried out for different grades of structural building and bridge steel specimens to establish the magnetic stress flux mathematical model, and the fitting equation of the magnetic flux changes with the positions of different sections of specimens was analyzed. Furthermore, a practical formula for stress detection was obtained through the experiments. Meanwhile, on these bases, the typical steel truss structure model of a Bailey beam was designed and manufactured under different working conditions, nondestructive online stress testing was carried out, and the stress of the model structure and its members was measured by strain and magnetic flux tests to obtain the curves of the test results for the stress-strain and magnetic stress flux, respectively. The results of these two methods are in good agreement with each other. The stress of the steel truss model structure was analyzed and calculated using the finite element method. The results agreed well with the experimental results from the magnetic stress sensing system-the maximum error was about 5%, which meets the requirements of engineering applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s20144043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412352PMC
July 2020

Antibacterial activity of ruthenium polypyridyl complexes against Staphylococcus aureus and biofilms.

J Biol Inorg Chem 2020 08 20;25(5):747-757. Epub 2020 Jun 20.

School of Pharmacy, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.

There is clearly a need for the development of new classes of antimicrobials to fight against multidrug-resistant bacteria. Here, we designed and synthesized of three ruthenium polypyridyl complexes: [Ru(bpy)(BTPIP)](ClO) (Ru(II)-1), [Ru(bpy)(ETPIP)](ClO) (Ru(II)-2) and [Ru(bpy)(CAPIP)](ClO) (Ru(II)-3) (N-N = bpy = 2,2'-bipyridine), their antimicrobial activities against S. aureus were assessed. The lead complexes of this set, Ru(II)-1(MIC = 0.016 mg/mL), was tested against biofilm. We also investigated whether bacteria can easily develop resistance to Ru(II)-1. The result demonstrated that S. aureus could not easily develop resistance to the ruthenium complexes. In addition, aimed to test whether ruthenium complexes treatment could increase the susceptibility of S. aureus to antibiotics, the synergism between Ru(II)-1 and common antibiotics against S. aureus were investigated using the checkerboard method. Interesting, Ru(II)-1 could increased the susceptibility of S. aureus to some aminoglycoside antibiotics(kanamycin and gentamicin). Finally, in vivo bacterial infection treatment studies were also conducted through murine skin infection model. These results confirmed ruthenium complexes have good antimicrobial activity in vitro and in vivo.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00775-020-01797-wDOI Listing
August 2020

Using a Two-Sex Life Table Tool to Calculate the Fitness of as a Predator of .

Insects 2020 Apr 30;11(5). Epub 2020 Apr 30.

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

A two-sex life table is a useful tool for studying the fitness of predators. Previous studies of Poppius (Heteroptera: Anthocoridae) fitness have not been done on (Lepidoptera: Gelechiidae) using a two-sex life table tool. This study reports the fitness of the minute predatory flower bug, when feeding on the cotton pest using a two-sex life table tool. Different densities (5, 10, and 15 eggs) of eggs were used to calculate the feeding capacity and fitness of in the laboratory at 28 °C ± 1, 75 ± 5% RH and 16:8 (L:D). The results concluded that is an efficient predator of . The maximum growth capacity of the predatory bug was attained when it was fed on 10 and 15 eggs. Furthermore, shorter generation and development time were also observed in the case of 15 eggs of . These results suggest that has considerable predatory potential and prefers feeding on eggs than on the first instar larvae at the fourth instar or the female stage. Although the field potential of is still unknown, this study will support future investigations in terms of field applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/insects11050275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290837PMC
April 2020

Diagnostic Yield and Performance of a Large Population-Based Cervical Cancer Screening Program in High-Risk Rural China.

J Cancer 2020 6;11(13):4000-4006. Epub 2020 Apr 6.

Department of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, China.

Primary cervical cancer screening by liquid-based cytology alone or with adjunctive HPV testing are available worldwide. However, little if any information is available about cervical cancer diagnostic yield of population-based cervical cancer screening in China. In response to it, we conducted a large prospective study on 40,000 women cervical cancer screening within six-month period in rural Shanxi Province, where has been reported as the highest cervical cancer rates in China. A standard cross-sectional survey by self-completed questionnaire was collected and followed by the liquid-based cytology screening. Follow-up biopsy with the diagnosis of cervical intraepithelial neoplasia 2 or higher lesion (CIN2+) were analyzed. Of initial 40,000 women participating in this study, 6.76% (2702/40,000) women had ASC-US or higher cytology screening results with ASC/SIL ratio at 6.14 (2381:388). Among them, 1.96% (782/40,000) women were found CIN lesions (95% CI, 1.68-2.23%) on confirmatory biopsies, including 0.55% (218/40,000) CIN2+ and 19 SCCa (47.5/100,000, 95% CI, 29-74/100,000). Women in Yangqu County had lower ASC/SIL ratio, but higher CIN2+ detection rate in comparison with that of Jiexiu County (6.69 vs. 8.84 and 56.7% vs. 43.9%), which reflects the cervical cancer distribution in different populations and regions. Analysis in age-stratified cytology results indicated women aged 60-65 years had the highest incidence of cytologic abnormality among all the age group; likewise, women aged >50 years were at higher risk in developing cervical high grade dysplasia or cancer than women aged <50 years (0.82% vs. 0.49%). This large-scale cervical cancer screening study provided important references as the instructive for establishing the nation-wide cervical cancer screening strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.41472DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171492PMC
April 2020

Effect of Cast Defects on the Corrosion Behavior and Mechanism of UNS C95810 Alloy in Artificial Seawater.

Materials (Basel) 2020 Apr 10;13(7). Epub 2020 Apr 10.

Dalian Marine Propeller Co., Ltd., Dalian 116021, China.

To study the effect of cast defects on the corrosion behavior and mechanism of the UNS C95810 alloy in seawater, an investigation was conducted by weight loss determination, scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), X-ray diffraction (XRD) and electrochemical testing of the specimen with and without cast defects on the surface. The results show that the corrosion rate of the alloy with cast defects is higher than that of the alloy without cast defects, but the defects do not change the composition of the resulting corrosion products. The defects increase the complexity of the alloy microstructure and the tendency toward galvanic corrosion, which reduce the corrosion potential from -3.83 to -86.31 mV and increase the corrosion current density from 0.228 to 0.23 μA⋅cm.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma13071790DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179039PMC
April 2020

Predictive value of p16, Ki-67 and ProExC immuno-qualitative features in LSIL progression into HSIL.

Exp Ther Med 2020 Apr 7;19(4):2457-2466. Epub 2020 Feb 7.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

The current nested case-control study was conducted to explore the prognostic value of cyclin-dependent kinase inhibitor 2A (p16), marker of proliferation Ki-67 (Ki-67) and immunohistochemical cocktail containing antibodies directed against topoisomerase IIα (TOP2A) and minichromosome maintenance 2 (MCM2) proteins (ProExC) immuno-qualitative features to predict low-grade squamous intraepithelial lesion (LSIL) progression. A total of 92 LSIL patients were followed-up for 2 years, where those with high-grade squamous intraepithelial lesion (HSIL) or persistent LSIL were designated as the case group and those who spontaneously regressed were designated as the control group. The infection status of human papillomavirus (HPV) was evaluated using flow-through hybridization and gene chip, whilst the expression of p16, Ki-67 and ProExC were tested in LSIL patient biopsies by immunohistochemistry. All data were collected at the beginning of the follow-up and patient outcomes were diagnosed by histopathological examination. To analyze the risk factors for LSIL progression, sensitivity, specificity, positive-negative predictive value (PPV-NPV), positive-negative likelihood ratio (PLR-NLR), Youden's index (YI) and multinomial logistic regression analysis was performed. The expression rates of p16, Ki-67, and ProExC were found to be higher in the progression group compared with those in the persistence and regression groups. Only p16 expression significantly associated with high-risk HPV infection. With respect to predicting HSIL, p16 staining was the most sensitive but Ki-67 staining was found to be the most specific. YI was the highest (42.1%) for p16 expression in the present study, followed by ProExC (39.5%) and Ki-67 (28.3%). However, the expression of ProExC was found to be an independent risk factor for LSIL progression into HSIL. In conclusion, whilst immunohistochemical staining for p16, Ki-67, and ProExC can be used to predict HSIL progression, only ProExC expression can be applied an independent risk factor for LSIL progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/etm.2020.8496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086290PMC
April 2020

High-power mode-locked thulium-doped fiber laser with tungsten ditelluride as saturable absorber.

Appl Opt 2020 Jan;59(1):196-200

A passively mode-locked thulium-doped fiber laser using a tungsten ditelluride saturable absorber (${{\rm WTe}_2}\mbox{-}{\rm SA}$WTe-SA) is demonstrated. High-power mode-locked pulses with an average output power of 108.1 mW were achieved by incorporating the ${{\rm WTe}_2}\mbox{-}{\rm SA}$WTe-SA into a thulium-doped fiber oscillator. To the best of our knowledge, this is the highest average power obtained from a ${{\rm WTe}_2}\mbox{-}{\rm SA}$WTe-SA-based fiber laser. We further amplified the output power to 5.60 W with an all-fiber thulium-doped double-cladding fiber amplifier. Our result indicates that ${{\rm WTe}_2}\mbox{-}{\rm SA}$WTe-SA could be an excellent candidate for a high-power fiber laser system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.59.000196DOI Listing
January 2020

Intranasal administration of dantrolene increased brain concentration and duration.

PLoS One 2020 11;15(3):e0229156. Epub 2020 Mar 11.

Department of Anesthesiology and Critical Care, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, United States of America.

Dantrolene has been demonstrated to be neuroprotective for multiple neurodegenerative diseases. However, dantrolene's limited penetration into the CNS hampers its effectiveness as a neuroprotective agent. Here, we studied whether the intranasal administration of dantrolene provided better penetration into the brain than the commonly used oral approach. C57BL/6 mice, aged 2-4 months, received a single dose of either intranasal or oral dantrolene (5mg/kg). Inhibition of dantrolene clearance from the brain was examined by co-administration with P-gp/BCRP inhibitors, nimodipine or elacridar. The concentration of dantrolene in the brain and plasma was measured at 10, 20, 30, 50, 70, 120, 150 and 180 minutes after administration. Separate cohorts of mice were given intranasal dantrolene (5mg/kg) or vehicle, 3 times/ week, for either 3 weeks or 4 months, to examine potential adverse side effects on olfaction and motor coordination, respectively. We found that Dantrolene concentrations were sustained in the brain after intranasal administration for 180 min, while concentrations fell to zero at 120 min for oral administration. Chronic use of intranasal dantrolene did not impair olfaction or motor function in these mice. Blood brain barrier pump inhibitors did not further increase dantrolene peak concentrations in the brain. Our results suggested that Intranasal administration of dantrolene is an effective route to increase its concentration and duration in the brain compared to the oral approach, without any obvious side effects on olfaction or motor function.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229156PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065741PMC
June 2020

Ultrafast fiber laser based on HfSe saturable absorber.

Nanotechnology 2020 Mar 26;31(24):245204. Epub 2020 Feb 26.

Shenzhen Key Laboratory of Laser Engineering, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, People's Republic of China. Sino-German College of Intelligent Manufacturing, Shenzhen Technical University, Shenzhen 518118, People's Republic of China.

We demonstrate the HfSe saturable absorber (SA) for the generation of ultrafast pulse laser. The HfSe SA device is fabricated by integrating HfSe nanosheets (NSs) with a microfiber. The material and optical characteristics of HfSe NSs show their high quality. The nonlinear optical absorption of HfSe SA is measured with a modulation depth of 5.8%. Stable soliton mode-locked laser based on HfSe SA is realized at the central wavelength of 1561.43 nm with pulse duration of 297 fs and the maximum pulse energy of 2.68 nJ. Our soliton fiber laser has a maximum output power of 48.5 mW with a high slope efficiency of 12.8%, which indicate that HfSe is a good candidate of SA for high efficient ultrashort pulses generation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ab7a2fDOI Listing
March 2020

Rapamycin-eluting Single and Double Stenting on Serum Markers in Coronary Bifurcation Lesions.

J Coll Physicians Surg Pak 2020 Feb;30(2):222-224

Department of Cardiology, Gansu Provincial Hospital, China.

The objective of study was to compare effects of rapamycin-eluting single and double stenting on serum markers like high sensitivity C-reactive protein (hs-CRP), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in patients with coronary bifurcation lesions. It was an experimental study carried out from April 2016 to July 2017. One hundred and twenty-six patients were divided into two equal groups according to different treatment regimens. Group A was treated with rapamycin-eluting single stenting and group B with rapamycin-eluting double stenting. Three months after operation, hs-CRP, TNF-α, IL-6, IL-8 and MCP-1 levels in group B were lower than those in group A (p=0.010, p <0.001, p <0.001, p <0.001 and p <0.001, respectively). After one year of follow-up, rate of intrasegmental restenosis of branch vessels was higher in group A than in group B (p=0.011). Compared with rapamycin-eluting single stenting, rapamycin-eluting double stenting may regulate more effectively the above serum markers levels, reduce the incidence of intrasegmental restenosis of branch vessels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.29271/jcpsp.2020.02.222DOI Listing
February 2020

Few-layer metal monochalcogenide saturable absorbers for high-energy Q-switched pulse generation.

Nanotechnology 2020 May 3;31(20):205204. Epub 2020 Feb 3.

Shenzhen Key Laboratory of Laser Engineering, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, People's Republic of China.

Two-dimensional layered materials have been widely utilized as nonlinear absorption materials to transfer continue-wave into pulse trains in fiber laser systems. Here, we prepare robust GaSe/GeSe composites with high power bearing capacity as saturable absorbers (SAs) and then investigate their nonlinear optical properties via broadband Z-scan measurement at 800 nm and 1550 nm, respectively. The modulation depths of GaSe/GeSe based SAs are measured to be 11.97% and 7.69% at 1550 nm. After incorporating the GaSe/GeSe SAs into an Erbium-doped fiber laser cavity, passively Q-switched pulse trains could be obtained with repetition rates changing from 83.58 to 136.78 kHz (70.41 to 161.65 kHz). The maximum output power and pulse energy are 52.1 mW/370.67 nJ (GaSe) and 21.6 mW/133.74 nJ (GeSe) under the maximum pump power of 600 mW. The results indicate that GaSe and GeSe possess outstanding thermal stability and could be employed as remarkable saturable absorption materials for high-energy pulses generation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ab7251DOI Listing
May 2020

Association between Vaginal Micro-environment Disorder and Cervical Intraepithelial Neoplasia in a Community Based Population in China.

J Cancer 2020 1;11(2):284-291. Epub 2020 Jan 1.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, China.

There are other factors that contribute to cervical carcinogenesis except HPV infection. This study aimed to investigate the association between vaginal micro-environment factors, including HO, vaginal PH value, vagina cleanness, β-glucuronidase, coagulase, neuraminidase and leukocyte esterase and cervical intraeipithelial neoplasia (CIN). In total 1019 participants, including 623 normal cervical (NC) women, 303 patients with low-grade cervical intraepithelial neoplasia (CIN1) and 93 patients with high-grade cervical intraepithelial neoplasia (CIN2/3), were enrolled into the study. HPV genotyping was detected by flow-through hybridization and gene chip. Vaginal HO, β-glucuronidase, coagulase, neuraminidase and leukocyte esterase were detected by Aerobic Vaginitis (AV) / Bacterial Vaginal Disease (BV) Five Joint Test Kit. Vaginal PH was measured on the glass slide after microscopy, using color strips with a PH range of 3.8-5.4. Vagina cleanness was determined according to the National Clinical Laboratory Practice Guideline. test and Logistic regression were operated using SPSS 22.0 software. Our results showed that HPV16 infection rate and the abnormal rates of HO, PH, vagina cleanness, β-glucuronidase or neuraminidase increased gradually along with the severity of CIN (<0.05). Abnormities of HO, cleanness, β-glucuronidase and neuraminidase were risk factors for CIN regardless of HPV16 infection, furthermore, abnormities of PH value, leukocyte esterase could also increase the risk of CIN in HPV16 positive group. In addition, women with abnormal vaginal micro-environment factors in HPV16 positive group had a significantly higher risk of developing CIN than HPV16 negative group. The results from generalized multifactor dimensionality reduction (GMDR) model showed that there was interaction effect with abnormities of vagina cleanness, HO, β-glucuronidase and neuraminidase on CIN2/3 in HPV16 negative group, while, there was interaction effect with abnormities of vagina cleanness, β-glucuronidase and neuraminidase on CIN1 and with abnormities of vagina cleanness, PH, HO, β-glucuronidase, neuraminidase and leukocyte esterase on CIN2/3 in HPV16 positive group. Our results suggested that vaginal micro-environment disorder could increase the risk of CIN, especially, the abnormality of HO, cleanness, β-glucuronidase and neuraminidase. There were interaction effects with abnormities of HO, vagina cleanness, β-glucuronidase and neuraminidase on CIN whether HPV16 was infected or not.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/jca.35022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930421PMC
January 2020

Bimodal Molecule as NIR-CT Contrast Agent for Hepatoma Specific Imaging.

Anal Chem 2020 01 20;92(1):1138-1146. Epub 2019 Dec 20.

School of Basic Medicine and Clinical Pharmacology , China Pharmaceutical University , Nanjing 211100 , China.

With currently available molecular imaging techniques, hepatocellular carcinoma (HCC), a liver cancer with high mortality rates and poor treatment responses, is mostly diagnosed at its late stage. This is largely due to the lack of highly sensitive contrast agents with high liver specificity. Herein, we report a novel bimodal contrast agent molecule CNCI-1 for the effective detection of HCC at its early stage both in vitro and in vivo. The agent has high liver specificity with effective X-ray computed tomography (CT)/near-infrared (NIR) imaging functions. It has been successfully applied to in vivo NIR imaging with high sensitivity and high selectivity to the HCC region of the HepG2 tumor-xenografted mice model and LM3 orthotopic hepatoma mice model. Moreover, the agent was found to be noninvasive and hepatocarcinoma cells preferential. Furthermore, it also enhanced the tumor imaging by revealing the blood vessels nearby for the CT image acquisition in the VX2 orthotopic hepatoma rabbit model. Our design strategy provides a new avenue to develop the medical relevant bimodal contrast agents for diagnosis of HCC at its early stage.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.9b04212DOI Listing
January 2020

Prevalence, genotype distribution and risk factors of cervical HPV infection in Yangqu, China: a population-based survey of 10086 women.

Hum Vaccin Immunother 2020 07 6;16(7):1645-1652. Epub 2019 Dec 6.

Department of Obstetrics and Gynecology, Second Hospital of Shanxi Medical University , Taiyuan, China.

Human papillomavirus(HPV) infection is a necessary factor for the development of cervical cancer. The HPV vaccine is currently available, but there is still a lack of large-scale research on the distribution and risk factors of HPV. The aim of this study is to investigate the genotype distribution and risk factors of HPV infection in Yangqu which is located in North China. This study enrolled 10086 women aged <65 years from Yangqu County. HPV genotypes were identified via standard HPV DNA testing. The overall prevalence of HPV infection was 8.92%. The prevalence of high-risk HPV types was 8.80%, and it was 0.38% for low-risk HPV types. Single genotype infection accounted for 67.91% in HPV-positive cases. The most common HPV genotypes were HPV-16, -52, and -58. HPV-18 was only the 11th most common type in HPV-positive cases. Women ≥50 years of age had the highest prevalence rate of HPV, and women <30 years had the lowest prevalence rate. The distribution of HPV genotypes also varied among the three age groups: <30, 30-49, and ≥50 years. The risk factors that contributed to the rate of HPV infection included low educational level, low income, smoking, age at first sexual encounter <23 years old, and number of births ≥3 times. This large routine clinical practice report of HPV prevalence and genotype distribution revealed the characteristics of HPV infection-type distributions in Shanxi Province, which should be considered in formulating comprehensive prevention strategies including vaccination for cervical cancer in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/21645515.2019.1689743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482860PMC
July 2020

Promising Hybrid Graphene-Silver Nanowire Composite Electrode for Flexible Organic Light-Emitting Diodes.

Sci Rep 2019 Nov 29;9(1):17998. Epub 2019 Nov 29.

State Key Laboratory of Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China.

Thanks to its high transparency, high carrier mobility, and thermal conductivity, graphene is often used as transparent conductive electrode (TCE) in optoelectronic devices. However, the low carrier concentration and high resistance caused by vacancy defects, grain boundaries, and superposed folds in typical graphene films limit its application. In this study, we propose a method to increase both the conductivity and carrier concentration in single-layer graphene (SLG) by blending it with silver nanowires (AgNWs). AgNWs provide connections between grain boundaries of graphene to improve charge-carrier transport. The AgNWs in this study can reduce the resistance of SLG from 650 Ω/◻ to 27 Ω/◻ yet still maintain a transmittance of 86.7% (at 550 nm). Flexible organic light-emitting diode, with a maximum 15000 cd m luminance was successfully fabricated using such graphene and AgNWs composite transparent electrodes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-54424-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884630PMC
November 2019