Publications by authors named "Jinping Wang"

176 Publications

Nanomedicine-Enabled Modulation of Tumor Hypoxic Microenvironment for Enhanced Cancer Therapy.

Adv Ther (Weinh) 2020 Jan 30;3(1). Epub 2019 Sep 30.

Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA; Department of Chemistry and Chemical Biology, Stevens Institute of Technology, Hoboken, NJ 07030, USA.

Hypoxia is a common condition of solid tumors that is mainly caused by enhanced tumor proliferative activity and dysfunctional vasculature. In the treatment of hypoxic human solid tumors, many conventional therapeutic approaches (e.g., oxygen-dependent photodynamic therapy, anticancer drug-based chemotherapy or X-ray induced radiotherapy) become considerably less effective or ineffective. In recent years, various strategies have been explored to deliver or generate oxygen inside solid tumors to overcome tumorous hypoxia and show promising evidence to improve the antitumor efficiency. In this review, the extrinsic regulation of tumor hypoxia via nanomaterial delivery is discussed followed by a summary of the mechanisms through which the modulated tumor hypoxic microenvironment improves therapeutic efficacy. The review concludes with future perspectives, to specifically address the translation of nanomaterial-based therapeutic strategies for clinical applications.
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http://dx.doi.org/10.1002/adtp.201900083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281934PMC
January 2020

Efficacy of lifestyle intervention in adults with impaired glucose tolerance with and without impaired fasting plasma glucose: a post hoc analysis of Da Qing Diabetes Prevention Outcome Study.

Diabetes Obes Metab 2021 Jul 1. Epub 2021 Jul 1.

Phoenix Epidemiology and Clinical Research Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Phoenix, Arizona, USA.

Aims: The extent that pre-diabetic fasting plasma glucose (FPG) levels influence the effectiveness of lifestyle interventions in preventing type 2 diabetes (T2DM) is uncertain. We aimed to determine if the outcome of lifestyle intervention in people with impaired glucose tolerance (IGT) differs in those with normal or impaired FPG levels.

Methods: Using data from the Da Qing Diabetes Prevention Outcome Study, a 30-year follow-up of a six-year randomized trial of lifestyle intervention in 576 people with IGT, we conducted a post-hoc analysis to compare the efficacy of intervention to reduce the incidence of T2DM and its complications in those with baseline FPG <100 mg/dL and FPG ≥100 mg/dL.

Results: Lifestyle intervention reduced the cumulative incidence of T2DM by 37-46% in those with baseline FPG <100 mg/dL and by 47-51% in those with FPG ≥100 mg/dL. The FPG <100 mg/dL group had a lower cumulative incidence of diabetes and 6.41 years median delay in its onset compared with 2.21 years delay in the FPG >100 mg/dL group. In those with FPG <100 mg/dL intervention was associated with at least as great a reduction in cardiovascular disease (CVD) and all-cause mortality as in the FPG >100 mg/dL group.

Conclusions: Lifestyle intervention reduced the incidence of T2DM in people with IGT regardless of baseline FPG levels, and in those with FPG <100 mg/dL led to a substantial delay in its onset. All persons with IGT, with normal or impaired FPG levels, may benefit from lifestyle intervention to delay its onset and mitigate the incidence of T2DM. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/dom.14481DOI Listing
July 2021

Leached starch content and molecular size during sorghum steaming for baijiu production is not determined by starch fine molecular structures.

Int J Biol Macromol 2021 Jun 9;184:50-56. Epub 2021 Jun 9.

School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Institutes of Agricultural Science and Technology Development of Yangzhou University, Yangzhou 225009, Jiangsu, China. Electronic address:

Sorghum steaming properties are important for both flavor and brewing efficiency of baijiu (Chinese alcohol liquor). However, it is currently unclear with respects to structural factors that affect sorghum steaming properties during baijiu production. In this study, starch fine molecular structures were characterized by size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis for 8 sorghum varieties used in baijiu production. Starch crystalline structures and ordering of double helices were characterized by the X-ray diffraction and differential scanning calorimetry. Results showed that only small differences were observed for starch molecular size distributions and chain-length distributions in the raw sorghum flour. Of significance, the leached starch content and molecular size during steaming was very different among these sorghum varieties. Furthermore, Spearman correlation analysis showed that there was no significant correlation between starch fine structural parameters with the leached starch content. On the other hand, the correlation analysis showed that leached starch molecular size was negatively correlated with starch crystallinity, while positively correlated with the onset and peak gelatinization temperatures. It is concluded that the sorghum steaming property is controlled by the starch crystalline structures instead of starch fine molecular structures. These results could help the baijiu industry to produce baijiu with more desirable properties.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.031DOI Listing
June 2021

Interference-free photoelectrochemical immunoassays using carboxymethylated dextran-coated and gold-modified TiO nanotube arrays.

Anal Bioanal Chem 2021 Jun 11. Epub 2021 Jun 11.

Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, Shandong, 250022, People's Republic of China.

An interference-free photoelectrochemical (PEC) immunoassay was developed for cardiac troponin I (cTnI) detection. Covalent linkage of cTnI antibody to carboxymethylated (CM-) dextran pre-immobilized onto a gold nanoparticles (AuNPs)-modified TiO nanotube array (NTA) affords five consecutive analyte captures with surface regenerations in between. Changes in the photocurrents at this photoanode before and after cTnI captures can be well fitted with the Langmuir isotherm from 0.220 pM to 2.20 nM cTnI. Owing to the inherently high sensitivity of the PEC detection, the detection limit (2.20 pg/mL) is lower than the range attainable with the enzyme-linked immunosorbent assay (ELISA) (6.00-40.0 pg/mL). Furthermore, CM-dextran prevents species in complex biological matrices from nonspecifically adsorbing onto the sensor surface, a feature not attainable with uncoated semiconductor electrodes or those coated with non-hydrogel-based chemical modifiers. The excellent anti-fouling property of dextran hydrogel allowed us to validate the accuracy of our regenerable sensors through a comparison of PEC immunoassays of patient sera to those of ELISA.
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http://dx.doi.org/10.1007/s00216-021-03442-0DOI Listing
June 2021

Losartan protects against osteoarthritis by repressing the TGF-β1 signaling pathway upregulation of PPARγ.

J Orthop Translat 2021 Jul 18;29:30-41. Epub 2021 May 18.

Department of Sports Medicine, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, Guangdong, 518035, China.

Objective: Losartan and activation of the peroxisome proliferator-activated receptor-γ (PPARγ) have been previously reported to alleviate the progression of osteoarthritis (OA). However, the nature of the interaction between losartan and PPARγ in OA remains elusive. Therefore, we aimed to investigate the mechanism of the regulation of PPARγ by losartan in the context of OA.

Methods: Clinical samples of OA patients were collected and the chondrocytes were further isolated, and used to construct OA chondrocyte model via induction with IL-1β. An OA mouse model was developed by the surgical destabilization of the medial meniscus (DMM). OA chondrocytes were treated with losartan, PPARγ siRNA and the PPAR- agonist GW1929 alone or in combination. Furthermore, the OA mice were treated with varying doses of losartan to determine the best mode of administration and treatment dose. Subsequently, the DMM mice were treated with losartan and GW9662. Expression of PPARγ, key proteins of the transforming growth factor-beta1 (TGF-β1) signaling pathway and the markers of OA degeneration were evaluated by the Western blot analysis, while effects on OA inflammatory factors were determined by ELISA.

Results: The downregulation of PPARγ and the upregulation of TGF-β1 signaling pathway were detected in the OA cartilage tissues and chondrocytes. Losartan treatment or PPARγ activation contributes to reduced levels of IL-6, IL-1β, TNF-α, and COX-2, expression of TGF-β1, MMP-13, ADAMTS-4, ADAMTS-5, HtrA1, and iNOS, along with reduced Smad2 and Smad3 phosphorylation, but elevated PPARγ and Collagen II expression and . Additionally, the intraarticular injection of losartan into the knee joint proved to be the best mode of administration, and 10 ​mg/mL being the optimal treatment concentration.

Conclusion: Our results show that losartan could arrest the progression of OA by upregulating PPARγ expression and inactivating the TGF-β1 signaling pathway.The translational potential of this article: Our results provide a biological rationale for the use of losartan as a potential candidate for OA treatment.
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http://dx.doi.org/10.1016/j.jot.2021.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142050PMC
July 2021

A deep learning approach for the fast generation of acoustic holograms.

J Acoust Soc Am 2021 Apr;149(4):2312

Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Acoustic holographic techniques are crucial in diverse applications, such as three-dimensional holographic display and particle manipulation. However, conventional methods for computer-generated acoustics holography rely heavily on iterative optimization algorithms, which are time-consuming and particularly hinder their capacity of generating a dynamic hologram in real time. Here, a deep learning approach based on U-Net is proposed to rapidly generate an acoustic hologram with optimal amplitude and phase maps. It is demonstrated that, after being trained with adequate data that are numerically synthesized by the pseudo-inverse method, the proposed deep learning approach can generate both amplitude and phase maps for new target images with an improved overall reconstruction quality. Remarkably, after the offline cost is compensated by a lower online cost for the proposed DL approach, the hologram generation speed is significantly accelerated by the proposed deep learning approach as compared with the pseudo-inverse method, especially for complicated or dynamic images. With the hierarchical feature learning capability and the fast online computational speed, the proposed deep learning approach can serve as a smart platform for rapidly generating complete maps of holograms for the sophisticated or dynamical target images, leading to the new possibility of real-time acoustic-hologram-based applications.
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http://dx.doi.org/10.1121/10.0003959DOI Listing
April 2021

Recent advances in porous nanostructures for cancer theranostics.

Nano Today 2021 Jun 8;38. Epub 2021 Apr 8.

Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, New Jersey, 07030, United States.

Porous nanomaterials with high surface area, tunable porosity, and large mesopores have recently received particular attention in cancer therapy and imaging. Introduction of additional pores to nanostructures not only endows the tunability of optoelectronic and optical features optimal for tumor treatment, but also modulates the loading capacity and controlled release of therapeutic agents. In recognition, increasing efforts have been made to fabricate various porous nanomaterials and explore their potentials in oncology applications. Thus, a systematic and comprehensive summary is necessary to overview the recent progress, especially in last ten years, on the development of various mesoporous nanomaterials for cancer treatment as theranostic agents. While outlining their individual synthetic mechanisms after a brief introduction of the structures and properties of porous nanomaterials, the current review highlighted the representative applications of three main categories of porous nanostructures (organic, inorganic, and organic-inorganic nanomaterials). In each category, the synthesis, representative examples, and interactions with tumors were further detailed. The review was concluded with deliberations on the key challenges and future outlooks of porous nanostructures in cancer theranostics.
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http://dx.doi.org/10.1016/j.nantod.2021.101146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059603PMC
June 2021

A randomized, double-blind, phase 2b proof-of-concept clinical trial in early Alzheimer's disease with lecanemab, an anti-Aβ protofibril antibody.

Alzheimers Res Ther 2021 04 17;13(1):80. Epub 2021 Apr 17.

Chambers-Grundy Center for Transformative Neuroscience, Department of Brain Health, School of Integrated Health Sciences, University of Nevada Las Vegas, Las Vegas, NV, USA.

Background: Lecanemab (BAN2401), an IgG1 monoclonal antibody, preferentially targets soluble aggregated amyloid beta (Aβ), with activity across oligomers, protofibrils, and insoluble fibrils. BAN2401-G000-201, a randomized double-blind clinical trial, utilized a Bayesian design with response-adaptive randomization to assess 3 doses across 2 regimens of lecanemab versus placebo in early Alzheimer's disease, mild cognitive impairment due to Alzheimer's disease (AD) and mild AD dementia.

Methods: BAN2401-G000-201 aimed to establish the effective dose 90% (ED90), defined as the simplest dose that achieves ≥90% of the maximum treatment effect. The primary endpoint was Bayesian analysis of 12-month clinical change on the Alzheimer's Disease Composite Score (ADCOMS) for the ED90 dose, which required an 80% probability of ≥25% clinical reduction in decline versus placebo. Key secondary endpoints included 18-month Bayesian and frequentist analyses of brain amyloid reduction using positron emission tomography; clinical decline on ADCOMS, Clinical Dementia Rating-Sum-of-Boxes (CDR-SB), and Alzheimer's Disease Assessment Scale-Cognitive Subscale (ADAS-Cog14); changes in CSF core biomarkers; and total hippocampal volume (HV) using volumetric magnetic resonance imaging.

Results: A total of 854 randomized subjects were treated (lecanemab, 609; placebo, 245). At 12 months, the 10-mg/kg biweekly ED90 dose showed a 64% probability to be better than placebo by 25% on ADCOMS, which missed the 80% threshold for the primary outcome. At 18 months, 10-mg/kg biweekly lecanemab reduced brain amyloid (-0.306 SUVr units) while showing a drug-placebo difference in favor of active treatment by 27% and 30% on ADCOMS, 56% and 47% on ADAS-Cog14, and 33% and 26% on CDR-SB versus placebo according to Bayesian and frequentist analyses, respectively. CSF biomarkers were supportive of a treatment effect. Lecanemab was well-tolerated with 9.9% incidence of amyloid-related imaging abnormalities-edema/effusion at 10 mg/kg biweekly.

Conclusions: BAN2401-G000-201 did not meet the 12-month primary endpoint. However, prespecified 18-month Bayesian and frequentist analyses demonstrated reduction in brain amyloid accompanied by a consistent reduction of clinical decline across several clinical and biomarker endpoints. A phase 3 study (Clarity AD) in early Alzheimer's disease is underway.

Trial Registration: Clinical Trials.gov NCT01767311 .
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http://dx.doi.org/10.1186/s13195-021-00813-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053280PMC
April 2021

A Sensitive Fluorescent Sensor for Highly Sensitive Detection of Water in Organic Solvents Based on Substituted Imidazole.

J Fluoresc 2021 Jul 7;31(4):907-913. Epub 2021 Apr 7.

College of Chemistry and Chemical Engineering, Qiqihar University, 161006, Qiqihar, People's Republic of China.

A novel substituted imidazole derivative 1a with carboxyl and quinoline structure has been designed and synthesized. And our initial discovery is that this compound can effectively detect water in ethanol. And the metal in solution couldn't affect the absorption and fluorescence Spectra of 1a. With the addition of water, the energy band appears a red-shift from 330nm to 355nm in the absorption spectra. And the emission spectrum undergoes an important change in its fluorescent effect in the presence of water. Furthermore, absorption peak of 1a displays a red-shift with increasing pH from 2.31 to 10.72. All available data (absorption and emission) strongly support the possible mechanism. Due to the pronounced fluorescence changing property, the substituted imidazole derivative 1a could be utilized as fluorescent probes for detecting water in ethanol.
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http://dx.doi.org/10.1007/s10895-021-02729-wDOI Listing
July 2021

Mechanical properties of de-epithelialized tracheal allografts.

J Thorac Dis 2021 Feb;13(2):1066-1074

Department of Otolaryngology, Tangdu Hospital, Air Force Medical University (Fourth Military Medical University), Xi'an, China.

Background: Many studies have shown that mucosa-treated tracheas can be used for allotransplantation without the use of immunosuppressants. However, no literature has reported whether the transplanted trachea has normal biomechanics. In this study, fresh dog trachea was used as a control to determine the mechanical properties of allograft trachea after mucous membrane removal.

Methods: Cervical tracheas (~10 cm in length, consisting of the cartilage rings down to the tracheal carina) and the surrounding connective tissues were removed from 20 mixed-breed dogs and then divided into 4 groups with 5 samples in each group. The groups were defined as follows: group A, fresh canine tracheas without any treatment; group B, canine tracheas after sodium dodecyl sulphate (SDS) de-epithelialization; group C, canine tracheas implanted into the dorsal fascia of beagle dogs for 1 month after SDS de-epithelialization; group D, canine tracheas implanted into the dorsal fascia of beagle dogs for 6 months after SDS de-epithelialization. All heterotopically implanted beagle dogs were free of any immunosuppressive treatment. Uniaxial tensile (n=20) and compressive (n=20) experiments were performed on the 4 groups of trachea using a universal testing machine; the tensile load was recorded at 150% of the original tracheal length (50% displacement), and the compressive load was recorded at 50% of the initial anteroposterior diameter (50% displacement). The average difference in tensile and compressive loads between groups was compared by analysis of variance.

Results: No significant difference was found in the mean compressive strength among groups (group A =2.38±0.87 N, group B =3.33±1.03 N, group C =5.39±2.44 N, group D =3.04±2.12 N; P=0.07 for all groups). The mean tensile strength of group C was 9.68±0.86 N, resulting in a significant difference in tensile strength compared to group A (22.51±3.91 N) and B (24.24±7.46 N) (P<0.001). No significant difference in tensile strength was observed among groups A, B, and D.

Conclusions: The de-epithelialized trachea showed no significant change in mechanical properties after 6 months of implantation.
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http://dx.doi.org/10.21037/jtd-20-2739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947527PMC
February 2021

Camouflaged Gold Nanodendrites Enable Synergistic Photodynamic Therapy and NIR Biowindow II Photothermal Therapy and Multimodal Imaging.

ACS Appl Mater Interfaces 2021 Mar 1;13(9):10778-10795. Epub 2021 Mar 1.

Department of Chemistry and Chemical Biology, Stevens Institute of Technology, Hoboken, New Jersey 07030, United States.

Gold nanodendrite (AuND)-based nanotheranostic agents with versatile capabilities were fabricated by optimizing the geometrical configurations (dendrite length and density) of AuND to achieve localized surface plasmon resonance (LSPR) in near-infrared biowindow II (NIR-II), and then subsequently functionalizing with a mitochondria-targeting compound (triphenylphosphonium, TPP), loading with an NIR-photosensitizer (indocyanine green, ICG) and coating with the macrophage cell membrane (MCM) to trap ICG within AuND and selectively interact with MDA-MB-231 cells. The novel [email protected] system enabled the integration of multimodal fluorescence/photoacoustic/surface-enhanced Raman imaging with synergistic therapies of NIR-II photothermal therapy and NIR-I photodynamic therapy for cancer treatment. Enhanced hyperthermia and elevated production of reactive oxygen species within the tumors MCM coating and mitochondria targeting afforded a synergistic efficacy for tumor eradication with limited side effects. The demonstrated biocompatibility, multi-imaging capability, and high therapeutic efficiency under NIR laser irradiation indicate the potentials of this multifunctional nanotheranostic platform for clinical utility in cancer therapy.
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http://dx.doi.org/10.1021/acsami.1c01238DOI Listing
March 2021

Associations of progression to diabetes and regression to normal glucose tolerance with development of cardiovascular and microvascular disease among people with impaired glucose tolerance: a secondary analysis of the 30 year Da Qing Diabetes Prevention Outcome Study.

Diabetologia 2021 Jun 19;64(6):1279-1287. Epub 2021 Feb 19.

Center of Endocrinology and Cardiovascular Metabolism, Fuwai Hospital, Beijing, China.

Aims/hypothesis: We aimed to determine associations of regression to normal glucose tolerance (NGT), maintaining impaired glucose tolerance (IGT) or progression to diabetes with subsequent risks of CVD and microvascular disease among Chinese adults with IGT.

Methods: We conducted an observational study among 540 participants in the Da Qing Diabetes Prevention Study, a 6 year lifestyle intervention trial in people with IGT, defined by 1985 WHO criteria as fasting plasma glucose <7.8 mmol/l and 2 h post-load plasma glucose ≥7.8 and <11.1 mmol/l. At the end of the trial, the groups that had regressed to NGT, remained with IGT or progressed to diabetes were identified. Participants were then followed for 24 years after completion of the trial, during which we compared the incidence and hazard ratios for CVD and microvascular disease in each group and estimated the differences in their median time to onset from parametric Weibull distribution models.

Results: At the end of the 6 year trial, 252 (46.7%) participants had developed diabetes, 114 (21.1%) had remained with IGT and 174 (32.2%) had regressed to NGT. Compared with those who developed diabetes during the trial, the median time to onset of diabetes was delayed by 14.86 years (95% CI 12.49, 17.25) in the NGT and 9.87 years (95% CI 8.12, 11.68) in the IGT groups. After completion of the trial, among those with diabetes, IGT and NGT, the 24 year cumulative incidence of CVD was 64.5%, 48.5% and 45.1%, respectively, and 36.8%, 21.7% and 16.5% for microvascular diseases. Compared with participants who had progressed to diabetes during the trial, those who regressed to NGT had a 37% (HR 0.63; 95% CI 0.47, 0.85) reduction in CVD incidence and a median delay of 7.45 years (95% CI 1.91, 12.99) in onset, and those who remained with IGT had a 34% (HR 0.66; 95% CI 0.47, 0.91) lower CVD incidence with a median delay in onset of 5.69 years (95% CI 1.0, 10.38). Participants with NGT had a 66% (HR 0.34; 95% CI 0.20, 0.56) lower incidence of microvascular diseases and a median delay in the onset of 18.66 years (95% CI 6.08, 31.24), and those remaining with IGT had a 52% (HR 0.48; 95% CI 0.29, 0.81) lower incidence with a median delay of 12.56 years (95% CI 2.49, 22.63).

Conclusions/interpretation: People with IGT who reverted to NGT or remained with IGT at the end of the 6 year trial subsequently had significantly lower incidences of CVD and microvascular disease than those who had developed diabetes.
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http://dx.doi.org/10.1007/s00125-021-05401-xDOI Listing
June 2021

Are We More Reactive to Persuasive Health Messages When They Appear in Our Customized Interfaces? The Role of Sense of Identity and Sense of Control.

Health Commun 2021 Feb 17:1-9. Epub 2021 Feb 17.

Donald P. Bellisario College of Communications, Pennsylvania State University.

Online users today are provided with a variety of customization tools to build their own information environment. A growing number of public health messages reach their target audiences via these digital venues. Given the deeply personal and individualized nature of customized environments, do online users show lesser reactance to persuasive health messages? Or, are they more likely to show reactance because they feel intruded upon? We conducted a 2 (Customization: present vs. absent) × 2 (Message threat: high vs. low) experiment (N = 145) to find out. Data showed that interface customization increases the sense of control and sense of identity among users. Sense of control is positively associated with threat to freedom of action, causing more affective reactance. In contrast, a sense of identity is negatively associated with anger toward the persuasive message. Theoretical and practical implications for online health campaigns are discussed.
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http://dx.doi.org/10.1080/10410236.2021.1885772DOI Listing
February 2021

Reconciling global mean and regional sea level change in projections and observations.

Nat Commun 2021 02 12;12(1):990. Epub 2021 Feb 12.

Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Laboratory of Physical Oceanography, Ocean University of China, Qingdao, China.

The ability of climate models to simulate 20th century global mean sea level (GMSL) and regional sea-level change has been demonstrated. However, the Intergovernmental Panel on Climate Change (IPCC) Fifth Assessment Report (AR5) and Special Report on the Ocean and Cryosphere in a Changing Climate (SROCC) sea-level projections have not been rigorously evaluated with observed GMSL and coastal sea level from a global network of tide gauges as the short overlapping period (2007-2018) and natural variability make the detection of trends and accelerations challenging. Here, we critically evaluate these projections with satellite and tide-gauge observations. The observed trends from GMSL and the regional weighted mean at tide-gauge stations confirm the projections under three Representative Concentration Pathway (RCP) scenarios within 90% confidence level during 2007-2018. The central values of the observed GMSL (1993-2018) and regional weighted mean (1970-2018) accelerations are larger than projections for RCP2.6 and lie between (or even above) those for RCP4.5 and RCP8.5 over 2007-2032, but are not yet statistically different from any scenario. While the confirmation of the projection trends gives us confidence in current understanding of near future sea-level change, it leaves open questions concerning late 21 century non-linear accelerations from ice-sheet contributions.
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http://dx.doi.org/10.1038/s41467-021-21265-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881246PMC
February 2021

Biomechanical changes of freezer-storaged and decellularized pig tracheal scaffoldings.

J Biomater Appl 2021 Apr 21;35(9):1208-1217. Epub 2021 Jan 21.

Department of Otolaryngology, the Second Affiliated Hospital, Air Force Medical University, Xi'an, China.

Background: As an excellent xenotransplant, the pig trachea can be decellularized and cryopreserved to reduce its immunogenicity. However, few reports are found on the changes of its mechanical properties after cryopreservation and decellularization.

Objective: To evaluate the structure and biomechanical properties in pig tracheal scaffolds resulting from decellularized and cryopreserved.

Material And Methods: Twenty-five pig tracheal segments were separated into five groups: untreated (group A), only decellularized (group B), only cryopreserved (group C), decellularized after cryopreserved (group D) and cryopreserved after decellularized (group E). Tracheal segments were subjected to uniaxial tension or compression using a universal testing machine to determine structural biomechanical changes.

Results: It showed that there was no statistically significant difference in the tensile strength of the trachea in each group. The compressive strength of group B, C and D were same as the group A ( > 0.05), while the group E was lower than that of the group A ( < 0.05).Conclusions and significance: The histological examination of the decellularization after cryopreservation shows that the removal of epithelial cells and submucosal cells is more thorough, and the biomechanical structure of the trachea is better preserved. This proved to be a new method to prepare xenotransplantation of trachea graft.
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http://dx.doi.org/10.1177/0885328220985662DOI Listing
April 2021

Prediction of 10-year mortality using hs-CRP in Chinese people with hyperglycemia: Findings from the Da Qing diabetes prevention outcomes study.

Diabetes Res Clin Pract 2021 Mar 14;173:108668. Epub 2021 Jan 14.

Endocrinology and Cardiovascular Metabolism Centre, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Aims: To examine whether high-sensitivity C-reactive protein (hs-CRP) can predict all-cause death in Chinese adults with hyperglycemia.

Methods: All the 237 diabetes and 49 prediabetes recruited in the study were evolved from the participants with impaired glucose tolerance in the original Da Qing Diabetes Study. Blood hs-CRP level was measured at 2006. Ten-year outcome of death was traced from 2006 to 2016. Cox model was used to analyse the association between hs-CRP level and the risk of all-cause death occurred over the subsequent 10 years.

Results: During the follow-up, death occurred in 36 (37.9%) subjects in the highest hs-CRP tertile group (hs-CRP > 2.16 mg/L) and 19 (20.0%) in the lowest hs-CRP tertile group (hs-CRP < 0.82 mg/L, p < 0.05). The corresponding incidence of all-cause death (per 1,000 person-years) was 44.7 (95% CI 30.1-59.3) and 21.6 (95% CI 11.9-31.3) in the two groups respectively (p < 0.0001). The highest hs-CRP tertile was associated with the increased risk of all-cause death significantly (hazard ratio 1.88, 95% CI 1.07-3.32) after controlling for traditional risk factors.

Conclusions: Serum hs-CRP was predictive of 10-year all-cause death in Chinese adults with hyperglycemia, suggesting the impact of low-grade inflammation on mortality deserves more attention.
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http://dx.doi.org/10.1016/j.diabres.2021.108668DOI Listing
March 2021

Long Non-Coding RNA 554 Promotes Cardiac Fibrosis via TGF-β1 Pathway in Mice Following Myocardial Infarction.

Front Pharmacol 2020 16;11:585680. Epub 2020 Dec 16.

Department of Cardiovascular Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangdong, China.

Cardiac fibrosis is observed in nearly every form of myocardial disease. Long non-coding RNAs (lncRNAs) have been shown to play an important role in cardiac fibrosis, but the detailed molecular mechanism remains unknown. We aimed at characterizing lncRNA 554 expression in murine cardiac fibroblasts (CFs) after myocardial infarction (MI) to identify CF-enriched lncRNA and investigate its function and contribution to cardiac fibrosis and function. In this study, we identified lncRNA NONMMUT022554 (lncRNA 554) as a regulator of MI-induced cardiac fibrosis. We found that lncRNA 554 was significantly up-regulated in the mouse hearts following MI. Further study showed that lncRNA 554 was predominantly expressed in cardiac fibroblasts, indicating a potential role of lncRNA 554 in cardiac fibrosis. knockdown of lncRNA 554 by siRNA suppressed fibroblasts migration and expression of extracellular matrix (ECM); while overexpression of lncRNA 554 promoted expression of ECM genes. Consistently, lentivirus mediated knockdown of lncRNA 554 could inhibit cardiac fibrosis and improve cardiac function in mouse model of MI. More importantly, TGF-β1 inhibitor (TEW-7197) could reverse the pro-fibrotic function of lncRNA 554 in CFs. This suggests that the effects of lncRNA 554 on cardiac fibrosis is TGF-β1 dependent. Collectively, our study illustrated the role of lncRNA 554 in cardiac fibrosis, suggested that lncRNA 554 might be a novel target for cardiac fibrosis.
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http://dx.doi.org/10.3389/fphar.2020.585680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7772239PMC
December 2020

IRF8 is crucial for the nicotine withdrawal-induced hyperalgesia in mice.

Transl Neurosci 2020 9;11(1):283-293. Epub 2020 Sep 9.

Department of Anesthesiology, Liaocheng People's Hospital, Cheeloo College of Medicine, Shandong University, Liaocheng, 252000, Shandong, China.

Background: Interferon regulatory factor 8 (IRF8) is involved in the pathogenesis of neuropathic pain. However, whether and how IRF8 can regulate the nicotine withdrawal (NTW)-induced hyperalgesia has not been clarified.

Methods: C57BL/6 mice were randomized and injected subcutaneously with saline (Control) or nicotine (3 mg/kg) three times per day for 7 consecutive days, followed by injection with mecamylamine to induce NTW. Their paw withdrawal latencies (PWLs) were measured, and the relative levels of IRF8 expression in the spinal cord tissues were determined longitudinally by western blot. The numbers of IRF8+ cells in the spinal cord tissues were examined. In addition, the NTW mice were randomized and infused intrathecally with vehicle saline (NS), control lentivirus or lentivirus for the expression of IRF8-specific shRNA for three days. Their PWLs, microglia activation, IRF8 and P2X4R and BDNF expression in the spinal cord tissues were determined.

Results: In comparison with the Control mice, the NTW significantly decreased the PWLs but increased the relative levels of IRF8 expression and the numbers of IRF8+ cells in the spinal cord tissues of mice. IRF8-silencing significantly mitigated the NTW-decreased PWLs and attenuated the NTW-enhanced microglia activation and P2X4R and BDNF expression in the spinal cord tissues of mice.

Conclusions: Spinal IRF8 is crucial for the NTW-induced hyperalgesia by enhancing microglia activation and spinal P2X4R and BDNF expression in mice. The IRF8/P2X4R/BDNF axis may be potential therapeutic targets for postoperative pain of smokers.
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http://dx.doi.org/10.1515/tnsci-2020-0139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712045PMC
September 2020

Affordable automated phenotypic antibiotic susceptibility testing method based on a contactless conductometric sensor.

Sci Rep 2020 12 4;10(1):21216. Epub 2020 Dec 4.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.

User-friendly phenotypic antibiotic susceptibility testing (AST) methods are urgently needed in many fields including clinical medicine, epidemiological studies and drug research. Herein, we report a convenient and cost-effective phenotypic AST method based on online monitoring bacterial growth with a developed 8-channel contactless conductometric sensor (CCS). Using E. coli and V. parahaemolyticus as microorganism models, as well as enoxacin, florfenicol, ampicillin, kanamycin and sulfadiazine as antibiotic probes. The minimum inhibitory concentration (MIC) determination was validated in comparison with standard broth microdilution (BMD) assay. The total essential agreements between the CCS AST assays and the reference BMD AST assays are 68.8-92.3%. The CCS has an approximate price of $9,000 (USD). Requiring neither chemical nor biotic auxiliary materials for the assay makes the cost of each sample < $1. The MICs obtained with the automated CCS AST assays are more precise than those obtained with the manual BMD. Moreover, in 72 percent of the counterpart, the MICs obtained with the CCS AST assays are higher than that obtained with the BMD AST assays. The proposed CCS AST method has advantages in affordability, accuracy, sensitivity and user-friendliness.
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http://dx.doi.org/10.1038/s41598-020-77938-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7718250PMC
December 2020

Evaluation of resource and energy utilization, environmental and economic benefits of rice water-saving irrigation technologies in a rice-wheat rotation system.

Sci Total Environ 2021 Feb 24;757:143748. Epub 2020 Nov 24.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address:

Currently, numerous challenges such as excessive irrigation water consumption, labor shortage, lower economic and environmental benefits pose serious threats to rice cultivation systems. Therefore, more water- and labor-efficient irrigation technologies are needed in rice production for minimal environmental hazards and greater economic benefits. After the screening experiment of water-saving cultivation technologies and cultivars, a two-year field experiment was conducted to further explore the effects of efficient water-saving technologies and rice cultivars on the comprehensive benefits, global warming potential (GWP), grain yield, economic benefits, water productivity, nitrogen partial factor productivity, radiation, accumulated temperature and energy use efficiency (EUE) of a rice-wheat rotation system. Conventional flooding irrigation (F), intermittent irrigation (IR), transplanting rainfed (TR) and rice dry cultivation (D) were implemented with two rice cultivars, including Hanyou73 (HY) and Huanghuazhan (HH). After rice harvest, a winter wheat cultivar (Huamai 2566) was planted with traditional methods. The system of rice dry cultivation and wheat rotation had higher comprehensive benefits, which were attributed to greater water productivity, economic benefits and EUE and lower GWP, especially in the rice growing season. D treatment enhanced the comprehensive and economic benefits by 2.5% and 26.8%, 1.6% and 11.3%, 3.3% and 0.6%, and reduced the GWP by 3.4%, 56.7% and 30.2% compared with F, IR and TR treatments in the rotation system, respectively. During the rice season, D treatment significantly (P < 0.05) increased the economic benefits, water productivity and EUE, but slight decreased the grain yield than other treatments. Overall, rice dry cultivation (especially with the HY cultivar) can achieve higher comprehensive benefits in rice growing season as well as in the whole rotation system.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143748DOI Listing
February 2021

Effect of Yugengtongyu Granules in Patients with Stable Coronary Artery Disease on Reducing Adverse Cardiovascular Events: A Double-Blind Controlled Trial.

J Altern Complement Med 2021 Feb 1;27(2):142-149. Epub 2020 Dec 1.

China-Japan Friendship Hospital, Beijing, China.

To evaluate the effect of Yugengtongyu granules on reducing the incidence of adverse cardiovascular events and improving quality of life (QOL) in patients with stable coronary artery disease (SCAD). A double-blind randomized controlled trial was conducted among SCAD population. One hundred fourteen patients were randomly assigned to experimental group ( = 57) and control group ( = 57) following randomized block design. Combined with the basis of standard treatment of SCAD, the experimental group and control group received Yugengtongyu granules or placebo, respectively, twice daily for 6 months and were followed for another 1 year (18 months in total from enrollment). Major outcomes (any occurrence of cardiovascular death, nonfatal myocardial infarction, or coronary revascularization), minor outcomes (any occurrence of all-cause death, ischemic stroke, readmission due to unstable angina, heart failure, or malignant arrhythmia), and composite outcomes (union of major and minor outcomes) were used to evaluate prognosis; Seattle Angina Questionnaire (SAQ) was applied to evaluate QOL, and levels of low density lipoprotein-cholesterol (LDL-C) and high sensitive C reacting protein (HS-CRP) in serum were tested. The incidence of composite outcomes in the experimental group was significantly lower than that in the control group (3 [5.2%] vs. 11 [19.2%], hazard ratio: 0.273, 95% confidence interval: 0.080-0.926,  = 0.022); major outcomes, minor outcomes, and independent events such as nonfatal myocardial infarction showed lowering trend in experimental group. Experimental group scored significantly higher than control group in four dimensions of SAQ: physical limitation, angina frequency, treatment satisfaction, and disease perception at the third- and sixth-month follow-up; there was no significant difference in serum level of LDL or HS-CRP at all scheduled timepoints. The addition of Yugengtongyu granules based on current standard treatment reduced the incidence of composite outcomes and improved QOL in patients with SCAD. The trial was registered in the Chinese Clinical Trial Registry (ChiCTR-TRC-13004370).
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http://dx.doi.org/10.1089/acm.2020.0361DOI Listing
February 2021

Novel Serotype of Epizootic Hemorrhagic Disease Virus, China.

Emerg Infect Dis 2020 12;26(12):3081-3083

In 2018, a strain of epizootic hemorrhagic disease virus (EHDV), named YNDH/V079/2018, was isolated from a sentinel calf in Mangshi County, Yunnan Province, China. Nucleotide sequencing and neutralization tests indicated that the virus belongs to a novel serotype of EHDV that had not been reported previously.
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http://dx.doi.org/10.3201/eid2612.191301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706924PMC
December 2020

Impact of prior endovascular interventions on outcomes of lower limb bypass surgery: A systematic review and meta-analysis.

Exp Ther Med 2020 Dec 27;20(6):259. Epub 2020 Oct 27.

Department of Vascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.

The aim of this meta-analysis was to evaluate the mortality, amputation and complication rates in patients with peripheral lower limb arterial disease undergoing bypass surgery with or without a prior history of endovascular operation. A systematic literature screen was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines on four academic databases, Medline, Scopus, Embase and Cochrane Central Register of Controlled Trials. Out of 1,072 records, six articles involving 11,420 patients (mean age, 68.1±2.0 years) met the inclusion criteria. The findings presented a 2b level of evidence (i.e. overall evidence represents data from individual cohort study or low quality randomized controlled trials) and suggested lower mortality, amputation and complication rates for patients undergoing bypass surgery without any history of endovascular operation, compared with those with a history of prior endovascular operation. Moreover, a random-effect meta-analysis suggested a small, positive reduction in mortality (Hedge's g=0.08), amputation (Hedge's g=0.18) and complication rates (Hedge's g=0.05) for patients undergoing bypass surgery without any history of endovascular operation. Nevertheless, owing to the scarcity of high-quality data, further studies and randomized clinical trials are needed to confirm these effects.
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http://dx.doi.org/10.3892/etm.2020.9389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668154PMC
December 2020

An ultrasmall chitosan nanosphere encapsulating carbon dots and rhodamine B as a ratiometric probe for the determination of Hg.

Mikrochim Acta 2020 Nov 12;187(12):655. Epub 2020 Nov 12.

College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

Hg-sensitive carbon dots (CDs) were synthesized by microwave-assisted pyrolysis of citric acid, sodium fluoride, and urea. The CDs as a signal report unit and rhodamine B (RhB) as a reference were then encapsulated in a nanosphere of chitosan assembled by a nonsolvent-induced chitosan colloidal formation and in situ cross-linking to construct a ratiometric probe for Hg (chitosan-CDs-RhB). Interestingly, without any assistance from acids to improve the solubility of chitosan, the nanosphere containing CDs and RhB had an ultrasmall size of 9.7 nm with only approximately 1.1-nm-thick layers of chitosan enclosing one dot. In order to keep the residual functional groups on the nanosphere from compromising the fluorescence response of CDs to Hg, Co was used as a fluorescently intact metal ion to saturate the functional groups. The saturated chitosan-CDs-RhB was thus potentially developed for determining Hg in the fruit bodies and mycelia of edible and medicinal fungi. Limits of detection (LODs) of 2.24, 5.29, and 2.03 μM and recoveries in the ranges 98.3 to 101.8%, 99.5 to 104.6%, and 97.4 to 100.9% were estimated for the determination of Hg in the fruit bodies of Pleurotus ostreatus, Lentinus edodes, and Hypsizygus marmoreus, respectively. Chitosan-CDs-RhB was further developed as a fluorescent ratiometric probe for quantitatively determining intracellular Hg in fungal mycelia with a linear calibration curve of R = - 0.145c + 1.69 within the range 0.013 to 0.356 μg g. Graphical abstract An ultrasmall chitosan nanosphere encapsulating carbon dots and rhodamine B as a ratiometric probe for the determination of Hg.
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http://dx.doi.org/10.1007/s00604-020-04627-7DOI Listing
November 2020

Construction of Pseudomolecules for the Chinese Chestnut () Genome.

G3 (Bethesda) 2020 10 5;10(10):3565-3574. Epub 2020 Oct 5.

Shandong Institute of Pomology, 66 Longtan Road, Tai'an Shandong, 271000, China

The Chinese chestnut ( Bl.) is a woody nut crop with a high ecological value. Although many cultivars have been selected from natural seedlings, elite lines with comprehensive agronomic traits and characters remain rare. To explore genetic resources with aid of whole genome sequence will play important roles in modern breeding programs for chestnut. In this study, we generated a high-quality genome assembly by combining 90× Pacific Biosciences long read and 170× high-throughput chromosome conformation capture data. The assembly was 688.93 Mb in total, with a contig N50 of 2.83 Mb. Most of the assembled sequences (99.75%) were anchored onto 12 chromosomes, and 97.07% of the assemblies were accurately anchored and oriented. A total of 33,638 protein-coding genes were predicted in the genome. Comparative genomic and transcriptomic analyses provided insights into the genes expressed in specific tissues, as well as those associated with burr development in the Chinese chestnut. This highly contiguous assembly of the genome provides a valuable resource for studies aiming at identifying and characterizing agronomical-important traits, and will aid the design of breeding strategies to develop more focused, faster, and predictable improvement programs.
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http://dx.doi.org/10.1534/g3.120.401532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7534444PMC
October 2020

[Observation on nystagmus characteristics and curative effect of patients with light cupulopathy].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2020 Jun;34(6):532-535

Department of Otology,First Hospital of Qinhuangdao,Qinhuangdao,066000,China.

To describe the characteristics of nystagmus in patients with Light Cupulopathy and to investigate its therapeutic effect. The Supine roll test in our hospital's otology clinic showed persistent DCPN in 26 patients, who received Supine roll test, null-point position test, and prone-supine test. All patients were treated with barbecue maneuver. The immediate, short-term and long-term effects of treatment were investigated. Null-point position on one side was present in 26 patients. The angles ranged from 15-45°, with an average of (26.34±8.78)°. Spontaneous nystagmus was observed in 30.8% ( 8/26 ) patients. The nystagmus was directed to the affected side in prone position and to the healthy side in supine position. Seventy-three percent of the patients had strong nystagmus and 26.9% had weak nystagmus in the Supine roll test. All patients received Barbecue maneuver, and the recovery rates were 0, 42.3% and 88.5% immediately, 1 week and 1 month after treatment respectively. There was a statistical difference in the recovery rate among immediate, one week and one month later (<0.01). The nystagmus performance of patients with persistent DCPN conforms to the light cupula hypothesis. The lesion side can be determined according to the null-point position and prone-supine test. The lesion side cannot be determined by the intensity of nystagmus in the Supine roll test in some cases. Repositioning maneuver is ineffective in treating light cupulopathy, but the disease is self-limited.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2020.06.013DOI Listing
June 2020

Evaluation of Salt-Induced Damage to Aged Wood of Historical Wooden Buildings.

Int J Anal Chem 2020 1;2020:8873713. Epub 2020 Aug 1.

Canadian Light Source, 44 Innovation Boulevard, Saskatoon, SK S7N 2V3, Canada.

Salt is a common cause of damage to building materials used in cultural and historical buildings. The damage to aged wood in historical wooden buildings has not been extensively studied, resulting in the need for a more detailed analysis. In this work, Yingxian Wooden Pagoda, a typical historical wooden structure, was taken as the research object. Multichemical analyses were conducted to evaluate and understand the salt-induced damage to the aged wood using a scanning electron microscope equipped with an energy-dispersive X-ray spectrometer, sulphur K-edge X-ray absorption near-edge structure spectroscopy, X-ray fluorescence spectroscopy, X-ray powder diffraction, and attenuated total reflectance fourier transformed infrared spectroscopy. The results showed the presence of invasive salt crystallisations and ions in the aged samples. The source of these invasive elements was deduced by identifying the type, amount, and valency of the elements; they were found to be derived from environmental factors such as acid rain and atmospheric pollutant. The unique damage mechanism and route induced by salt in historical buildings made of wood were summarised; the damage was attributed to the accumulation of sulphate salt causing hydrolysis of the carbohydrates and salt crystallisation resulting in mechanical damage. This interdisciplinary study is significant for decision making in studies related to the preservation and evaluation of historical wooden buildings.
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http://dx.doi.org/10.1155/2020/8873713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7416263PMC
August 2020

Coexistence of Low-Grade Fetal Adenocarcinoma and Adenocarcinoma in situ of the Lung Harboring Different Genetic Mutations: A Case Report and Review of Literature.

Onco Targets Ther 2020 7;13:6675-6680. Epub 2020 Jul 7.

Department of Pathology, College of Basic Medical Sciences and the First Affiliated Hospital, China Medical University, Shenyang, People's Republic of China.

Low-grade fetal lung adenocarcinoma (L-FLAC) is an exceptionally rare pulmonary tumor, presenting with unclear histological and molecular features. In particular, the potential driver genes of L-FLAC remain elusive. To date, only five reports have documented genetic aberrations in L-FLAC. In the current study, we describe an unusual case of L-FLAC coexisting with adenocarcinoma in situ (AIS) of the lung, harboring different genetic mutations. A 39-year-old non-smoker female patient was referred to our hospital with the chief complaint of dyspnea for 20 days. Chest computed tomography (CT) revealed a 2.5×1.5×1.5 cm nodule in the right middle lobe, with no mediastinal lymph node enlargement or distant metastases. Thoracoscopic surgery was performed to remove the nodules. Histopathological analysis of the tissue sections, based on findings from immunohistochemical staining, confirmed a diagnosis of L-FLAC coexisting with AIS of the lung. Next-generation sequencing revealed L-FLAC-based mutations in and , and AIS harboring mutations. Currently, the patient remains recurrence-free 17 months after the initial diagnosis. This report presents the first case demonstrating the coexistence of L-FLAC and AIS in the same pulmonary nodule, harboring different genetic mutations. Based on the literature review, this is the second reported case of L-FLAC bearing mutations.
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http://dx.doi.org/10.2147/OTT.S260993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354012PMC
July 2020

Metabolomic analysis reveals metabolites and pathways involved in grain quality traits of high-quality rice cultivars under a dry cultivation system.

Food Chem 2020 Oct 18;326:126845. Epub 2020 Apr 18.

College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, Hubei 434025, China. Electronic address:

Dry cultivation of high-yielding or drought-resistant rice cultivars relieves the current pressure on rice cultivation systems. However, the metabolites and metabolic pathways that affect rice quality in high-yield or drought-resistant rice cultivars under dry cultivation have not yet been explored. A field experiment was conducted in 2017 to explore the effects of flooding irrigation (F) and dry cultivation (D) of high-yield and -quality cultivars (Huanghuazhan, HH; Yueyou9113, YY) and a drought-resistant cultivar (Hanyou73, HY) on rice quality traits using metabolomic analysis. Treatment DHH maintained higher head rice yield, amylose content, protein content, and breakdown values and a lower whiteness index and setback value than other cultivars under dry cultivation. These rice quality traits were related to 16 metabolites and 8 metabolic pathways. DHH showed decreases in stress response metabolites (m72, m98, m127, m165, m167, m213, m297, and m298) but maintained stress resistance (m29, m30, m39, m48, and m58) and sucrose (m150) accumulation in grains to support head rice yields and a low whiteness index. Raising the lactose, choline, and nicotinoylcholine levels in rice grains improved their protein content and cooking quality. DHH also adjusted the glycine, serine and threonine metabolism, galactose metabolism, and starch and sucrose metabolism of rice. This affected the biosynthesis of anthocyanin, phenylpropanoid, and flavonoid, supporting protein biosynthesis and starch accumulation in the endosperm. These findings provide further possibilities for improving rice quality traits of high-yield and -quality rice cultivars under dry cultivation.
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http://dx.doi.org/10.1016/j.foodchem.2020.126845DOI Listing
October 2020

Dual-emission carbon dots achieved by luminescence center modulation within one-pot synthesis for a fluorescent ratiometric probe of pH, Hg, and glutathione.

Mikrochim Acta 2020 05 15;187(6):330. Epub 2020 May 15.

Shandong Province Key Laboratory of Applied Mycology, College of Chemical and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.

Dual-emission carbon dots were synthesized by one-pot hydrothermal pyrolysis of citric acid and polyethyleneimine in the presence of rhodamine B at 160 °C for 5 h. The carbon dots have an average diameter of 2.51 nm with rhodamine moiety on their surface. Two emission bands centered at 447 and 581 nm are exhibited in their fluorescence spectra excited at 360 nm, and the former is sensitive while the latter is insensitive to Hg and pH. Glutathione (GSH) can recover the fluorescence quenched by Hg. Therefore, the dual-emission carbon dots were developed as a fluorescent ratiometric probe employing the ratio of the two intensities at 447 and 581 nm (R) as the signal for the determinations of pH, Hg, and GSH. In the range of 5.0-10.0, a good linear relationship between R and pH was built with a regression equation of R = 11.95-0.56 pH (R = 0.998). In the range from 0.0 to 8.0 μM, an excellent linear relationship between R and the concentration of Hg was obtained with a calibration equation of R = 6.2317-0.4458c (R = 0.995) and a limit of detection (LOD) of 0.24 μM. In the range from 1.0 to 10.0 μM, a linear equation, R = 1.9133-0.4157c (R = 0.995), was calibrated between R and the concentration of glutathione with a LOD of 0.27 μM. The recoveries for the determinations of Hg and GSH in real samples were in the ranges of 94.6 to 103.8% and 94.3 to 104.2%, respectively. Graphical abstract Dual-emission carbon dots achieved by luminescence center modulation within one-pot synthesis for a fluorescent ratiometric probe of pH, Hg, and glutathione.
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http://dx.doi.org/10.1007/s00604-020-04311-wDOI Listing
May 2020