Publications by authors named "Jinlong Ma"

90 Publications

Chemotherapeutic drug-induced immunogenic cell death for nanomedicine-based cancer chemo-immunotherapy.

Nanoscale 2021 Oct 13. Epub 2021 Oct 13.

College of Pharmacy, Weifang Medical University, Weifang 261053, China.

Chemotherapy has been a conventional paradigm for cancer treatment, and multifarious chemotherapeutic drugs have been widely employed for decades with significant performances in suppressing tumors. Moreover, some of the antitumor chemotherapeutic agents, such as doxorubicin (DOX), oxaliplatin (OXA), cyclophosphamide (CPA) and paclitaxel (PTX), can also tackle tumors through the induction of immunogenic cell death (ICD) in tumor cells to trigger specific antitumor immune responses of the body and improve chemotherapy efficacy. In recent years, chemo-immunotherapy has attracted increasing attention as one of the most promising combination therapies to struggle with malignant tumors. Many effective antitumor therapies have benefited from the successful induction of ICD in tumors, which could incur the release of endogenous danger signals and tumor-associated antigens (TAAs), further stimulating antigen-presenting cells (APCs) and ultimately initiating efficient antitumor immunity. In this review, several well-characterized damage-associated molecular patterns (DAMPs) were introduced and the progress of ICD induced by representative chemotherapeutic drugs for nanomedicine-based chemo-immunotherapy was highlighted. In addition, the combination strategies involving ICD cooperated with other therapies were discussed. Finally, we shared some perspectives in chemotherapeutic drug-induced ICD for future chemo-immunotherapy. It was hoped that this review would provide worthwhile presentations and enlightenments for cancer chemo-immunotherapy.
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http://dx.doi.org/10.1039/d1nr05512gDOI Listing
October 2021

Association between endometrial thickness and birth weight in fresh IVF/ICSI embryo transfers: a retrospective cohort study of 9273 singleton births.

Reprod Biomed Online 2021 Aug 30. Epub 2021 Aug 30.

Center for Reproductive Medicine, Reproductive Hospital Affiliated to Shandong University, No.157 Jingliu Road, Jinan 250000, China; National Research Centre for Assisted Reproductive Technology and Reproductive Genetic, Jinan, China. Electronic address:

Research Question: What is the association between endometrial thickness (EMT) on HCG trigger day and outcomes related to birth weight in fresh IVF and intracytoplasmic sperm injection (ICSI) embryo transfer cycles?

Design: A retrospective cohort study of 9273 singleton live births born to women undergoing fresh IVF/ICSI cycles in a single centre between January 2014 and December 2018. Multivariable logistic regression was used to investigate the associations between EMT, low birth weight (LBW) and small for gestational age (SGA). Multivariable-adjusted linear regression models incorporating restricted cubic splines were used to investigate the dose-response relationship between EMT, birth weight and birth weight z-score, respectively. An EMT of 8 mm was set as a reference value.

Results: Compared with women with an EMT measuring between 8 mm or less and less than 14 mm, the risk of delivering a SGA infant was higher when EMT measured less than 8.0 mm (adjusted OR 1.78, 95% CI 1.09 to 2.90) and lower when EMT measured 14.0 mm or above (adjusted OR 0.57, 95% CI 0.35 to 0.93, respectively). Compared with women with an EMT of 8.0 mm, women with an EMT of 5.0, 6.0 and 7.0 mm were associated with a decrease of 120 g (95% CI -175 g to -66 g), 80 g (95% CI -116 g to -44 g), and 40 g (95% CI -58 g to -22 g) in birth weight; and a decrease of 0.19 (95% CI -0.27 to -0.10), 0.12 (95% CI -0.18 to -0.07) and 0.06 (95% CI -0.09 to -0.03) in birth weight z-score, respectively.

Conclusions: A thinner endometrium was associated with lower birth weight and birth weight z-score, and higher risk of SGA. Women with a thin endometrium warrant special attention during pregnancy.
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http://dx.doi.org/10.1016/j.rbmo.2021.08.021DOI Listing
August 2021

Effect of hydrogenation on the thermal conductivity of 2D gallium nitride.

Phys Chem Chem Phys 2021 Oct 13;23(39):22423-22429. Epub 2021 Oct 13.

State Key Laboratory of Coal Combustion, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

The indirect bandgap of two-dimensional GaN hinders its application in the optical field. Hydrogenation can convert the bandgap type of the GaN monolayer from an indirect to a direct one and also tune the bandgap size. The thermal transport, an important property in the application of two-dimensional materials, is also influenced by hydrogenation. By performing first-principles calculations and solving the phonon Boltzmann equation, we investigate the effect of hydrogenation on the thermal conductivity of the GaN monolayer. The results show that hydrogenation will slightly increase the thermal conductivity of the GaN monolayer from 70.62 Wm K to 76.23 Wm K at 300 K. The little effect of hydrogenation on thermal conductivity is mainly dominated by two competing factors: (1) the reduction of ZA mode lifetime due to the breaking of reflection symmetry after hydrogenation and (2) the increased contribution from TA and LA modes due to the reduction of anharmonic scattering caused by the enlarged phonon bandgap after hydrogenation. The results are compared with other two-dimensional materials with hexagonal monolayer structures.
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http://dx.doi.org/10.1039/d1cp02759jDOI Listing
October 2021

An integrated RNA-Seq and network study reveals that valproate inhibited progesterone production in human granulosa cells.

J Steroid Biochem Mol Biol 2021 Sep 3;214:105991. Epub 2021 Sep 3.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, 250012, China. Electronic address:

Background: Valproate (VPA) is an antiepileptic drug (AEDs) with an ideal effect against epilepsy as well as other neuropsychiatric diseases. There is considerable evidence that women taking VPA are prone to reproductive endocrine disorders. However, few studies have been published about VPA effects on human ovarian granulosa cells.

Methods: By treating human ovarian granulosa cell line KGN with VPA, the cell viability and progesterone production function were evaluated. RNA-sequencing was applied to uncover the global gene expression upon VPA treatment.

Results: We revealed that VPA dose-dependently repressed the viability of KGN. VPA treatment at 600 μM inhibited the progesterone production. The mRNA and protein expression of CYP11A1 and STAR, two key enzymes in the biosynthesis of progesterone, were both suppressed. Gene set enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis of the transcriptome revealed classical functions of VPA as a neuromodulator and regulator of histone acetylation modifications. In addition to this, VPA commonly affected many steroid metabolism related genes in follicle cells, such as promoting the expression of vitamin D receptor (VDR).

Conclusion: Our findings suggest that VPA caused steroids metabolism pathways disturbance related with ovarian function and inhibited progesterone biosynthesis by inhibiting the expression of steroidogenesis genes. Our research may provide theoretical basis for the better use of VPA and the possible ways to counteract its side effects.
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http://dx.doi.org/10.1016/j.jsbmb.2021.105991DOI Listing
September 2021

Differential Lipidomic Characteristics of Children Born to Women with Polycystic Ovary Syndrome.

Front Endocrinol (Lausanne) 2021 9;12:698734. Epub 2021 Aug 9.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

Objective: To describe the lipidomic characteristics of offspring born to polycystic ovary syndrome (PCOS) women (PCOS-off) and assess the associations between differential lipids and clinical phenotypes.

Methods: Ultra performance liquid chromatography and mass spectrometry were performed on plasma samples from 70 PCOS-off and 71 healthy controls. The associations of differential metabolites with clinical phenotypes were examined by multiple linear regression.

Results: Forty-four metabolites were significantly altered in PCOS-off, including 8 increased and 36 decreased. After stratification according to sex, 44 metabolites (13 increased and 31 decreased) were expressed differently in girls born to PCOS women (PCOS-g), most of which were glycerolipids. Furthermore, 46 metabolites (9 increased and 35 decreased) were expressed differently in boys born to PCOS women (PCOS-b), most of which were glycerophospholipids. Significant associations of metabolites with weight Z-score and high density lipoprotein cholesterol were found in PCOS-off. Triglycerides, low density lipoprotein cholesterol, and thyroid-stimulating hormone were separately correlated with some lipids in PCOS-g and PCOS-b.

Conclusions: PCOS-off showed specific lipid profile alterations. The abnormal level of glycerophospholipids and sphingomyelin indicated the risk of glucose metabolism and cardiovascular diseases in PCOS-off. Some lipids, such as phosphatidylcholines, lysophosphatidylcholine and sphingomyelin, may be the potential markers. The results broadened our understanding of PCOS-offs' cardiometabolic status and emphasized more specific and detailed monitoring and management in this population.
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http://dx.doi.org/10.3389/fendo.2021.698734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8380809PMC
August 2021

Selective thermotherapy of tumor by self-regulating photothermal conversion system.

J Colloid Interface Sci 2022 Jan 30;605:752-765. Epub 2021 Jul 30.

College of Pharmacy, Weifang Medical University, Weifang 261053, China; Collaborative Innovation Center for Target Drug Delivery System, Weifang Medical University, Weifang 261053, China; Shandong Engineering Research Center for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang 261053, China. Electronic address:

One major challenge of photothermal therapy (PTT) is achieving thermal ablation of the tumor without damaging the normal cells and tissues. Here, we designed a self-regulating photothermal conversion system for selective thermotherapy based on self-assembling gold nanoparticles (S-AuNPs) and investigated the selectivity effect using a novel home-made in vitro selective photothermal transformation model and an in vivo skin damaging assessment model. In the in vitro selective photothermal transformation model, laser irradiation selectively increased the temperature of the internal microenvironment (pH 5.5) and resulted in an obvious temperature difference (ΔT ≥ 5 °C) with that of the external environment (pH 7.4). More importantly, in the in vivo skin damaging assessment model, S-AuNPs achieved good tumor inhibition without damaging the normal skin tissue compared with the conventional photothermal material. This work provides not only a novel validation protocol for tumor thermotherapy to achieve the biosafety of specifically killing tumor cells and normal tissue but also an evaluation methodology for other precise therapy for cancers.
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http://dx.doi.org/10.1016/j.jcis.2021.07.134DOI Listing
January 2022

Berberine for bone regeneration: Therapeutic potential and molecular mechanisms.

J Ethnopharmacol 2021 Sep 29;277:114249. Epub 2021 May 29.

College of Pharmacy, Weifang Medical University, Weifang, 261053, PR China; Collaborative Innovation Center for Target Drug Delivery System, Weifang Medical University, Weifang, 261053, Shandong, PR China; Shandong Engineering Research Center for Smart Materials and Regenerative Medicine, Weifang Medical University, Weifang, Shandong, 261053, PR China. Electronic address:

Ethnopharmacological Relevance: Berberine is a quaternary ammonium isoquinoline alkaloid, mainly extracted from plants berberaceae, papaveraceae, ranunculaceae and rutaceae such as coptis chinensis Franch, Phellodendron chinense, and berberis pruinosa. The plants are extensively used in traditional medicine for treating infection, diabetes, arrhythmia, tumor, osteoporosis et al. Pharmacological studies showed berberine has effects of anti-inflammation, anti-tumor, lower blood lipid, lower blood glucose, anti-osteoporosis, anti-osteoarthritis et al. AIM OF THE STUDY: This review aims to summarize the application of natural herbs that contain berberine, the further use and development of berberine, the effects as well as mechanism of berberine on osteoblasts and osteoclasts, the recent advances of in vivo studies, in order to provide a scientific basis for its traditional uses and to prospect of the potential applications of berberine in clinics.

Method: The research was achieved by retrieving from the online electronic database, including PubMed, Web of Science, Google Scholar and China national knowledge infrastructure (CNKI). Patents, doctoral dissertations and master dissertations are also searched.

Results: Berberine has a long history of medicinal use to treat various diseases including bone disease in China. Recent studies have defined its function in promoting bone regeneration and great potential in developing new drugs. But the systemic mechanism of berberine on bone regeneration still needs more research to clarify.

Conclusion: This review has systematically summarized the application, pharmacological effects, mechanism as well as in vivo studies of berberine and herbs which contain berberine. Berberine has a definite effect in promoting the proliferation and differentiation of osteoblasts as well as inhibiting the production of osteoclasts to promote bone regeneration. However, the present studies about the system mechanisms and pharmacological activity of berberine were incomplete. Applying berberine for new drug development remains an exciting and promising alternative to bone regeneration engineering, with broad potential for therapeutic and clinical practice.
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http://dx.doi.org/10.1016/j.jep.2021.114249DOI Listing
September 2021

Analysis of STAG3 variants in Chinese non-obstructive azoospermia patients with germ cell maturation arrest.

Sci Rep 2021 05 12;11(1):10077. Epub 2021 May 12.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, 44 Wenhua Xi Road, Jinan, 250012, Shandong, China.

STAG3 is essential for male meiosis and testis of male Stag3 mice shows the histopathological type of germ cell maturation arrest (MA). Whether variants of the STAG3 gene exist in Chinese idiopathic non-obstructive azoospermia (NOA) patients needs to be determined. We recruited 58 Chinese NOA men with MA who underwent testis biopsy and 192 fertile men as the control group. The 34 exons of the STAG3 gene were amplified using polymerase chain reaction (PCR) and sequenced. We identified eight novel single nucleotide polymorphisms (SNPs), including two missense SNPs (c.433T > C in exon2 and c.553A > G in exon3), three synonymous SNPs (c.539G > A, c.569C > T in exon3, and c.1176C > G in exon8), and three SNPs in introns. The allele and genotype frequencies of the novel and other SNPs have no significant differences between two groups. Our results indicated that variants in the coding sequence of the STAG3 gene were uncommon in NOA patients with MA in Chinese population. Future studies in large cohorts of different ethnic populations will be needed to determine the association between the STAG3 gene and NOA.
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http://dx.doi.org/10.1038/s41598-021-89559-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115624PMC
May 2021

Thin endometrium is associated with the risk of hypertensive disorders of pregnancy in fresh IVF/ICSI embryo transfer cycles: a retrospective cohort study of 9,266 singleton births.

Reprod Biol Endocrinol 2021 Apr 9;19(1):55. Epub 2021 Apr 9.

Center for Reproductive Medicine, Shandong University, No.157 Jingliu Road, Jinan, 250021, China.

Background: Thin endometrial thickness (EMT) has been suggested to be associated with reduced incidence of pregnancy rate after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment, but the effect of thin endometrium on obstetric outcome is less investigated. This study aims to determine whether EMT affects the incidence of obstetric complications in fresh IVF/ICSI-embryo transfer (ET) cycles.

Methods: We conducted a retrospective cohort study collecting a total of 9266 women who had singleton livebirths after fresh IVF/ICSI-ET treatment cycles at the Center for Reproductive Medicine Affiliated to Shandong University between January 2014 and December 2018. The women were divided into three groups according to the EMT: 544 women with an EMT ≤8 mm, 6234 with an EMT > 8-12 mm, and 2488 with an EMT > 12 mm. The primary outcomes were the incidence of obstetric complications including hypertensive disorders of pregnancy (HDP), gestational diabetes mellitus (GDM), placental abruption, placenta previa, postpartum hemorrhage (PPH) and cesarean section. Multivariable logistic regression analysis was performed to calculate the odds ratios (ORs) and 95% confidence intervals (CIs) for associations between the EMT measured on the day of human chorionic gonadotropin (HCG) trigger and the risk of the outcomes of interest.

Results: The HDP incidence rate of pregnant women was highest in EMT ≤ 8 mm group and significantly higher than those in EMT from > 8-12 mm and EMT > 12 mm group, respectively (6.8% versus 3.6 and 3.5%, respectively; P = 0.001). After adjustment for confounding variables by multivariate logistic regression analysis, a thin EMT was still statistically significant associated with an increased risk of HDP. Compared with women with an EMT > 8-12 mm, women with an EMT ≤8 mm had an increased risk of HDP (aOR = 1.853, 95% CI 1.281-2.679, P = 0.001).

Conclusion: A thin endometrium (≤8 mm) was found to be associated with an increased risk of HDP after adjustment for confounding variables, indicating that the thin endometrium itself is a risk factor for HDP. Obstetricians should remain aware of the possibility of HDP when women with a thin EMT achieve pregnancy through fresh IVF/ICSI-ET treatment cycles.
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http://dx.doi.org/10.1186/s12958-021-00738-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034143PMC
April 2021

Ultrahigh Thermal Conductivity of θ-Phase Tantalum Nitride.

Phys Rev Lett 2021 Mar;126(11):115901

Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.

Extracting long-lasting performance from electronic devices and improving their reliability through effective heat management requires good thermal conductors. Taking both three- and four-phonon scattering as well as electron-phonon and isotope scattering into account, we predict that semimetallic θ-phase tantalum nitride (θ-TaN) has an ultrahigh thermal conductivity (κ), of 995 and 820  W m^{-1} K^{-1} at room temperature along the a and c axes, respectively. Phonons are found to be the main heat carriers, and the high κ hinges on a particular combination of factors: weak electron-phonon scattering, low isotopic mass disorder, and a large frequency gap between acoustic and optical phonon modes that, together with acoustic bunching, impedes three-phonon processes. On the other hand, four-phonon scattering is found to be significant. This study provides new insight into heat conduction in semimetallic solids and extends the search for high-κ materials into the realms of semimetals and noncubic crystal structures.
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http://dx.doi.org/10.1103/PhysRevLett.126.115901DOI Listing
March 2021

Spatio-temporal distribution and source identification of heavy metals in particle size fractions of road dust from a typical industrial district.

Sci Total Environ 2021 Aug 16;780:146357. Epub 2021 Mar 16.

School of Information and Safety Engineering, Zhongnan University of Economics and Law, Wuhan 430073, China; Research Center for Environment and Health, Zhongnan University of Economics and Law, Wuhan 430073, China.

Seasonally distribution and source apportionment of Cr, Mn, Ni, Cu, Zn, Cd, and Pb in the road dust (RD) with the four size fraction sizes (<45 μm, 45-63 μm, 63-150 μm and all sizes) in a typical industrial district were investigated using a combination of Moran index, Principal component analysis (PCA), and Positive matrix factorization (PMF). Results showed that from winter to summer, the proportion of the <45 μm fraction dust in the total RD mass increased from 6.72% to 15.92% and that of 63-150 μm dust particles decreased from 31.13% to 21.76%. The proportion of the enrichment factors (EF) at moderate pollution level in winter was higher than that in summer, especially for Cu, Cd and Pb. Further, the heavy metals were relatively enriched in particles 63-150 μm in summer, while in particles <45 μm in winter. Spatially, the distribution of heavy metal concentrations was more concentrated in the winter and showed low levels of regional diffusion. Based on the pollution mapping and PCA-PMF, the integrated source appointment showed that the industrial sources are the main sources of Zn, Cd and Pb, and their contributions are higher at a particle below 45 μm in winter. The construction source significantly influenced Cr, Mn and Cu in summer with little diversity among particle size ranges. Therefore, the <45 μm particles from industrial emission in winter is suggested to be under priority control. And the industrial transformation demonstration area in the Qingshan district should upgrade heavy pollution industry lines and strengthen the monitoring of soot emissions. Further, emissions from coal-fired enterprises should be restricted in winter. Besides, the attention should be paid to avoid urban traffic jams around construction projects and increase enclosed construction ratio.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146357DOI Listing
August 2021

Fabrication and Characterization of Chitosan/Poly(Lactic-Co-glycolic Acid) Core-Shell Nanoparticles by Coaxial Electrospray Technology for Dual Delivery of Natamycin and Clotrimazole.

Front Bioeng Biotechnol 2021 5;9:635485. Epub 2021 Mar 5.

College of Pharmacy, Weifang Medical University, Weifang, China.

Natamycin (NAT) is the drug of choice for the treatment of fungal keratitis (FK). However, its inherent shortcomings, such as poor solubility, high dosing frequency, and long treatment cycle, need to be urgently addressed by designing a new delivery to widen its clinical utility. Growing research has confirmed that clotrimazole (CLZ) plays a significant role in fungal growth inhibition. Hence, coaxial electrospray (CO-ES) technology is used herein to prepare nano-systems with an average hydrodynamic particle size of 309-406 nm for the co-delivery of NAT and CLZ in chitosan (CTS) and poly(lactic-co-glycolic acid) (PLGA). The resulting NAT/[email protected]/PLGA formulations were characterized by a transmission electron microscope (TEM) and release test. The results show that the formulations had obvious core-shell structures, uniform particle distribution, and also can sustain the release of drugs over 36 h. Furthermore, hemolysis, corneal irritation test, local allergenic test, and antifungal activity analyses are performed to evaluate the safety and efficiency of the formulations. Thus, good biosafety along with a significant anti-candidiasis effect are found in the NAT/[email protected]/PLGA nanoparticles (NPs). Taken together, the results suggest that this design may provide a promising drug delivery system and a new option for the treatment of FK.
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http://dx.doi.org/10.3389/fbioe.2021.635485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973235PMC
March 2021

Factors Influencing the Live Birth Rate Following Fresh Embryo Transfer Cycles in Infertile Women After Endometrioma Cystectomy.

Front Med (Lausanne) 2021 25;8:622087. Epub 2021 Feb 25.

School of Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

Reproductive outcomes after fresh fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET) cycles are diverse in infertile women with a history of ovarian cystectomy for endometriomas. We aimed to develop a logistic regression model based on patients' characteristics including number of embryos transferred and stimulation protocols to predict the live birth rate in fresh IVF/ICSI-ET cycles for such patients. We recruited 513 infertile women with a history of ovarian cystectomy for endometriomas who underwent their first fresh ET with different stimulation protocols following IVF/ICSI cycles in our unit from January 2014 to December 2018. One or two embryo are implanted. Clinical and laboratory parameters potentially affecting the live birth rate following fresh ET cycles were analyzed. Univariable and multivariable analyses were performed to assess the relationship between predictive factors and live birth rate. The overall live birth rate was 240/513 (46.8%). Multivariable modified Poisson regression models showed that two factors were significantly lowers the probability of live birth: female age ≥ 5 years (aOR 0.603; 95% CI 0.389-0.933; = 0.023); BMI range 21-24.99 kg/m compared with BMI <21 kg/m (aOR 0.572; 95% CI 0.372-0.881, = 0.011). And two factors significantly increased the probability of live birth: AFC >7 (aOR 1.591; 95% CI 1.075-2.353; = 0.020); two embryos transferred (aOR 1.607; 95% CI 1.089-2.372; = 0.017). For these infertile women who had undergone ovarian cystectomy for endometriosis, female age <35 years, AFC > 7, and two embryos transferred might achieve better clinical fresh IVF/ICSI-ET outcomes. BMI <21 kg/m or ≥25 kg/m might also have positive effects on the live birth rate, but different ovarian stimulation protocols had no significant effects. However, a larger sample size may be needed for further study.
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http://dx.doi.org/10.3389/fmed.2021.622087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947290PMC
February 2021

Structure and electronic bandgap tunability of -plane GaN multilayers.

Phys Chem Chem Phys 2021 Mar;23(9):5431-5437

State Key Laboratory of Coal Combustion, School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Two-dimensional (2D) gallium nitride (GaN) has attracted a lot of attention due to its promising applications in photoelectric nano-devices. Most previous research studies have focused on polar c-plane 2D structures. Here, by employing first principles calculations, we systematically investigate the structural and electronic properties of non-polar m-plane GaN with different numbers of atomic layers. The results show a layer-dependent structure transition and electronic band variation for m-plane GaN. It is found that the monolayer keeps a planar hexagonal structure due to sp2 hybridization, whereas the multilayers are formed by stacking of buckled hexagonal monolayers with unsaturated coordination number at the surface sublayer and bulk-like inner layers. These discrepancies in the structure further induce an indirect to direct transition of the band gap type when the layer number reaches twelve. By carefully examining the relationship between the structure and electronic bandgap, we find that the indirect bandgap comes from the unsaturated surface with a planar like structure. On surface modification, saturation of the surface dangling bonds results in an indirect to direct band gap transition.
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http://dx.doi.org/10.1039/d0cp06093cDOI Listing
March 2021

The aggravated short-term PM-related health risk due to atmospheric transport in the Yangtze River Delta.

Environ Pollut 2021 Apr 5;275:116672. Epub 2021 Feb 5.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, China; Institute of Eco-Chongming (IEC), Shanghai, 202162, China. Electronic address:

Severe fine particulate matter (PM) pollution and the associated health risks remain pressing issues in the Yangtze River Delta (YRD), although significant efforts have been made locally, such as the Clean Air Action since 2013. Regional transport is an important contributor to high PM levels during haze episodes in the YRD, but its impact on human health is rarely analyzed. In this study, we evaluate the short-term PM-related health risks and associated economic losses due to different source regions by estimating daily mortality based on model results in the YRD. The results show that regional transport induces significant health risks in the YRD during haze days, contributing over 60% of daily premature mortality in Shanghai and Nanjing (major cities in the YRD). Moreover, in Hangzhou and Jiaxing, regional transport's contribution can be as high as 70%. The total daily mean economic loss in the YRD is estimated as 526.8 million Chinese Yuan (approximately 81.4 million U.S. dollar) in winter of 2015 and 2016, accounting for 1.4% of the daily averaged gross domestic product (GDP) of the YRD. Emission control (in accordance with the 13th Five-year Energy Conservation and Emission Reduction Plan) is an effective way to reduce health risks in the YRD, reducing premature deaths during haze days by 12-33%. More stringent emission control measures are suggested for further reduce PM-related health risks.
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http://dx.doi.org/10.1016/j.envpol.2021.116672DOI Listing
April 2021

Enhanced atmospheric oxidation capacity and associated ozone increases during COVID-19 lockdown in the Yangtze River Delta.

Sci Total Environ 2021 May 7;768:144796. Epub 2021 Jan 7.

Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China; Institute of Eco-Chongming (IEC), Shanghai 200062, China. Electronic address:

Aggressive air pollution control in China since 2013 has achieved sharp decreases in fine particulate matter (PM), along with increased ozone (O) concentrations. Due to the pandemic of coronavirus disease 2019 (COVID-19), China imposed nationwide restriction, leading to large reductions in economic activities and associated emissions. In particular, large decreases were found in nitrogen oxides (NO) emissions (>50%) from transportation. However, O increased in the Yangtze River Delta (YRD), which cannot be fully explained by changes in NO and volatile organic compound (VOCs) emissions. In this study, the Community Multi-scale Air Quality model was used to investigate O increase in the YRD. Our results show a significant increase of atmospheric oxidation capacity (AOC) indicated by enhanced oxidants levels (up to +25%) especially in southern Jiangsu, Shanghai and northern Zhejiang, inducing the elevated O during lockdown. Moreover, net P(HO) of 0.4 to 1.6 ppb h during lockdown (Case 2) was larger than the case without lockdown (Case 1), mainly resulting in the enhanced AOC and higher O production rate (+12%). This comprehensive analysis improves our understanding on AOC and associated O formation, which helps to design effective strategies to control O.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787908PMC
May 2021

A Rapid and Simple Method for Lithium Purification and Isotopic Analysis of Geological Reference Materials by MC-ICP-MS.

Front Chem 2020 23;8:557489. Epub 2020 Nov 23.

State Key Laboratory of Isotope Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.

A simple method has been developed to purify lithium (Li) from matrix elements in geological reference materials, using a single-column packed with AGMP-50 cation exchange resin, followed by high-precision Li isotope measurements by multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS). A series of tests, such as different types of resin, loading amount of Li, loading volumes, and various eluents, were conducted to ascertain the optimal conditions for Li purification and the effects of intensity, acidity, and presence of potential matrix elements on Li isotope measurements were also evaluated. In our experiment, Al and high-field-strength elements (HFSEs), such as Ti, Zr, and Hf, were eluted by 0.2 M HCl + 0.3 M HF, and 0.73 M HCl was used to separate Li from other matrix elements, such as Na. This method is suitable for processing large amount of Li (60-270 ng) and enabling a Li recovery of close to 100%, with effective removal of matrix elements such as Na and Ca. Besides, our method achieves low matrix interferences (e.g., Na/Li << 1 and Ca/Li << 1 for rock and seawater via a single-column procedure; Ca/Li < 2 for carbonate via a two-column procedure) and also uses small volume of eluents and is rapid (~5 h), enabling a total separation to be completed in ~0.5 d. Using this method, we report Li isotopic compositions of various geological reference materials, including igneous rocks, seawater, and carbonate. The Li isotopic compositions are consistent with the data published previously for the analyzed reference materials. As such, the reported method is ideally suited for Li separation from multiple types of geological samples prior to isotopic analysis.
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http://dx.doi.org/10.3389/fchem.2020.557489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719721PMC
November 2020

Fresh versus frozen embryo transfer in women with thin endometrium: a retrospective cohort study.

Ann Transl Med 2020 Nov;8(21):1435

Department of Pathology, Reproductive Hospital Affiliated to Shandong University, Jinan, China.

Background: A thin endometrium affects embryo implantation. We designed a retrospective cohort study to analyze the differences of fertilization-intracytoplasmic sperm injection (IVF-ICSI) outcomes between fresh embryo transfer (ET) and frozen ET in patients with a thin endometrium.

Methods: The present study comprised of 1,110 patients with a thin endometrium undergoing IVF-ICSI between January 2013 and December 2017 in our unit. Propensity score matching (PSM) was used to balance the influence of intergroup confounding factors and to compare the pregnancy outcomes of the matched patients in the two groups. The fresh embryo group and frozen embryo group consisted of 632 and 478 women, respectively. After PSM, 173 patients were included in the two groups, respectively.

Results: The frozen embryo group showed a higher live birth rate (31% 18.4%, P<0.001; 30.6% 19.7%, P=0.019), clinical pregnancy rate (40% 26.4%, P<0.001; 38.7% 25.4%, P=0.008), and biochemical pregnancy rate (46.2% 32.9%, P<0.001; 44.5% 31.8%, P=0.020) than the fresh embryo group before and after PSM.

Conclusions: Our results demonstrated that for women with a thin endometrium who were undergoing IVF, the live birth rate, clinical pregnancy rate, and biochemical pregnancy rate after frozen ET were significantly higher than in the fresh ET group.
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http://dx.doi.org/10.21037/atm-20-3230DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723638PMC
November 2020

Responses of decline in air pollution and recovery associated with COVID-19 lockdown in the Pearl River Delta.

Sci Total Environ 2021 Feb 26;756:143868. Epub 2020 Nov 26.

Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China; Institute of Eco-Chongming (IEC), Shanghai 200062, China. Electronic address:

The Guangdong government implemented lockdown measures on January 23, 2020, to ease the spread of the coronavirus disease 2019 (COVID-19). These measures prohibit a series of human activities and lead to a great reduction in anthropogenic emissions. Starting on February 20, all companies resumed work and production, and emissions gradually recovered. To investigate the response of air pollutants in the Pearl River Delta (PRD) to the emission reduction and recovery related to COVID-19 lockdown, we used the Community Multi-scale Air Quality (CMAQ) model to estimate the changes in air pollutants, including three periods: Period I (January 10 to January 22, 2020), Period II (January 23 to February 19, 2020), Period III (February 20 to March 9, 2020). During Period II, under the concurrent influence of emissions and meteorology, air quality improved significantly with PM, NO, and SO decreased by 52%, 67%, and 25%, respectively. O had no obvious changes in most cities, which mainly due to the synergetic effects of emissions and meteorology. In Period III, with the recovery of emissions and the changes in meteorology, the increase of secondary components was faster than that of primary PM (PPM), which indicated that changes in PPM concentration were more sensitive to emissions reduction. O concentration increased as emission and temperature rising. Our findings elucidate that more effective emission control strategies should be implemented in PRD to alleviate the increasingly serious pollution situation.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688412PMC
February 2021

Activin A increases human trophoblast invasion by upregulating integrin β1 through ALK4.

FASEB J 2021 02 23;35(2):e21220. Epub 2020 Nov 23.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China.

Activin A promotes human trophoblast invasion during the first trimester of pregnancy and is associated with preeclampsia and pregnancy-induced hypertension (PE/PIH) in naturally conceived pregnancies. However, whether integrin β1 mediates activin A-increased trophoblast invasion remains unknown and the evidence is limited regarding the predictive value of activin A for PE/PIH in women receiving in vitro fertilization (IVF) treatment. Here, we studied the role and underlying molecular mechanisms of integrin β1 in activin A-promoted invasion in immortalized (HTR8/SVneo) and primary human extravillous trophoblast (EVT) cells. A nest case-control study was designed to investigate the predictive/diagnostic value of activin A in IVF pregnancies. Results showed that integrin β1 expression increased after activin A treatment and knockdown of integrin β1 significantly decreased both basal and activin A-increased HTR8/SVneo cell invasion. SB431542 (TGF-β type I receptors inhibitor) abolished activin A-induced SMAD2/SMAD3 phosphorylation and integrin β1 overexpression. Activin A-upregulated integrin β1 expression was attenuated after the depletion of ALK4 or SMAD4 in both HTR8/SVneo and primary EVT cells. Furthermore, we found similar first-trimester activin A levels in IVF patients with or without subsequent PE/PIH. These results reveal that integrin β1 mediates activin A-promoted trophoblast invasion through ALK4-activated SMAD2/3-SMAD4 pathway, and the predictive/diagnostic value of first-trimester maternal serum activin A for hypertensive disorders of pregnancy might be different in IVF population.
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http://dx.doi.org/10.1096/fj.202001604RDOI Listing
February 2021

Down-Regulation of C3aR/C5aR Inhibits Cell Proliferation and EMT in Hepatocellular Carcinoma.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820970668

Department of Hepatobiliary Surgery, 105002General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.

Complement 3a (C3a) and complement 5a (C5a), small cleavage fragments generated by complement activation, has been previously shown to be obviously up-regulated in highly metastatic hepatocellular carcinoma (HCC) cells. However, their functional roles in HCC cells remains unclear. Here, we investigated the biological function of G protein-coupled receptor C3aR/C5aR using small interference RNA in HCC cells. Our data showed that C3aR and C5aR knockdown significantly inhibited the proliferation, migration and invasion of HCC cells using CCK-8, colony formation and transwell assays. Flow cytometry assay showed C3aR and C5aR knockdown induced cell cycle G0/G1 phase arrest and apoptosis in HCC cells. Moreover, we found down-regulation of C3aR/C5aR obviously down-regulated the expression of PCNA, Ki-67 and suppressed the epithelial-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin and vimentin) in HCC cells. Collectively, our data demonstrated that targeting C3aR/C5aR may hold promise for the treatment of HCC.
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http://dx.doi.org/10.1177/1533033820970668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672723PMC
November 2020

Cryptic t(15;17) acute promyelocytic leukemia with a karyotype of add(11)(p15) and t(13,20)- A case report with a literature review.

Bosn J Basic Med Sci 2021 Apr 1;21(2):246-251. Epub 2021 Apr 1.

Department of Hematology, Zhongda Hospital, School of Medicine, Southeast University, Institute of Hematology Southeast University, Nanjing, China.

Most acute promyelocytic leukemia (APL) are characterized by reciprocal translocations t(15;17)(q22;21), which results in the fusion of PML gene at 15q22 with RARα gene at 17q21. However, several complex variant translocations also have been reported. Here we report a 62-year-old man with typical morphology and clinical features of APL with a complex karyotype including add(11)(p15) and t(13,20)(q12;q11.2) without typical t(15;17) assayed by the G-banding analysis. FISH with a PML/RARα dual-color DNA probe showed an atypical fusion signal, RT-qPCR analysis showed PML/RARα fusion transcripts, and NGS detected FLT3, WT1, and KRAS mutations. The patient achieved complete remission after treatment with conventional chemotherapy combined ATRA and ATO. Although the mechanism of this kind of cryptic variant remains unknown, we conclude that the cryptic PML/RARα fusion with add(11)(p15), t(13,20)(q12;q11.2) seems not to alter the effectiveness of chemotherapy combined with ATRA and ATO.
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http://dx.doi.org/10.17305/bjbms.2020.5106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982059PMC
April 2021

Differential expression profile of plasma exosomal microRNAs in women with polycystic ovary syndrome.

Fertil Steril 2021 03 9;115(3):782-792. Epub 2020 Oct 9.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, People's Republic of China; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, People's Republic of China; Shandong Key Laboratory of Reproductive Medicine, Jinan, People's Republic of China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, People's Republic of China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, People's Republic of China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, People's Republic of China; Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Objective: To examine different expression profiles of plasma exosomal microRNA (miRNA) in polycystic ovary syndrome (PCOS) patients and controls, and their potential roles in PCOS pathogenesis.

Design: Experimental study.

Setting: Center for reproductive medicine.

Patient(s): Seventy-five PCOS patients and 75 age-matched controls.

Intervention(s): Plasma exosomes miRNAs sequenced from 15 PCOS patients and 15 controls.

Main Outcome Measure(s): Plasma exosomal miRNA expression and the correlation between PCOS phenotypes and miRNA expression.

Result(s): The sequenced plasma exosomes miRNAs were further determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in a larger cohort, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Correlation analysis and receiver operating characteristic (ROC) curve analysis were used to determine the association between PCOS phenotypes and miRNA expression. The miRNA sequencing revealed 34 exosomal miRNAs were differentially expressed between PCOS patients and controls. Via qRT-PCR, five differentially expressed miRNAs (miR-126-3p, miR-146a-5p, miR-20b-5p, miR-106a-5p, and miR-18a-3p) were identified. The GO and KEGG analyses predicted their target functions included axon guidance, mitogen-activated protein kinase (MAPK) signaling, endocytosis, circadian rhythms, and cancer pathways. The expression of these miRNAs correlated with menstrual cycle, antral follicle count, hormone level, and combined yielded a ROC curve area of 0.781 in discriminating PCOS patients from the controls.

Conclusion(s): Differential expression of plasma exosomal miRNAs may confer a risk of PCOS and may be helpful in distinguishing PCOS patients from controls. Certain miRNA expression may associated to the disease progression, which could help in an epigenetic understanding of the pathophysiology of PCOS.
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http://dx.doi.org/10.1016/j.fertnstert.2020.08.019DOI Listing
March 2021

Lattice Thermal Transport in Monolayer Group 13 Monochalcogenides MX (M = Ga, In; X = S, Se, Te): Interplay of Atomic Mass, Harmonicity, and Lone-Pair-Induced Anharmonicity.

Inorg Chem 2020 Oct 29;59(20):14899-14909. Epub 2020 Sep 29.

Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.

We perform a systematic study of the lattice dynamics and the lattice thermal conductivity, κ, of monolayer group 13 monochalcogenides MX (M = Ga, In; X = S, Se, Te) by combining an iterative solution for linearized phonon Boltzmann transport equation and density functional theory. Among the competing factors influencing κ, harmonic parameters along with the atomic masses dominate over anharmonicity. An increase in atomic mass leads to a decrease in phonon frequencies and phonon group velocities and consequently in κ. At = 300 K, the calculated κ values are 54.9, 48.1, 44.3, 25.0, 22.3, and 17.3 W m K for GaS, InS, GaSe, InSe, GaTe, and InTe monolayers, respectively. Further analysis of anharmonic scattering rates and average scattering matrix elements evidences that the anharmonicity characterized by the third-order IFCs in GaS and InS are the largest among all monolayer group 13 monochalcogenides despite the largest κ values. This is attributed to a strong interaction between nonbonding lone-pair s electrons around the S atom and adjacent bonding electrons. In addition, the κ of these monolayers further reduces to 50% for sample sizes 300-400 nm. Our findings provide fundamental insights into thermal transport in monolayer group 13 monochalcogenides and should stimulate further experimental exploration of thermal transport in these materials for possible theromoelectric and thermal management applications.
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http://dx.doi.org/10.1021/acs.inorgchem.0c01407DOI Listing
October 2020

Strain-Induced Ultrahigh Electron Mobility and Thermoelectric Figure of Merit in Monolayer α-Te.

ACS Appl Mater Interfaces 2020 Sep 15;12(39):43901-43910. Epub 2020 Sep 15.

Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China.

In line with the classic phonon-glass electron-crystal (PGEC) paradigm, semiconducting and semimetallic multinary compounds remain the cornerstone of the state-of-the-art thermoelectric materials. By contrast, elemental PGEC is very rare. In this work, we report a thermoelectric study of monolayer α-Te by first-principles calculations and solving the parameter-free Boltzmann transport equation. It is found that monolayer α-Te possesses high electron mobility (about 2500 cm V s) at room temperature due to small effective mass, low phonon frequencies, and thus a restricted phase space for electron-phonon scattering. In monolayer α-Te, the electrons near the conduction band edge are mainly scattered by the heavily populated quadratically dispersing out-of-plane acoustic (ZA) phonon modes. The thermoelectric figure of merit () for -type monolayer α-Te is 0.55 at 300 K and 1.46 at 700 K. Notably, tensile strain stiffens the ZA modes, yielding a linear energy-momentum dispersion relation and the removal of the diverging thermal population of ZA phonons. Consequently, the electron mobility is enhanced. At a 4% tensile strain, the electron mobility can reach up to 8000 cm V s at room temperature while the thermal conductivity is almost unaffected, yielding a state-of-the-art value of 0.94 and 2.03 in n-type monolayer α-Te at 300 and 700 K, respectively. For completeness, the thermoelectric study of p-type monolayer α-Te is also conducted. These results beckon further experiments toward high-performance α-Te-based thermoelectric materials via doping, alloying, and compositing.
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http://dx.doi.org/10.1021/acsami.0c10236DOI Listing
September 2020

Verification of a ZBTB16 variant in polycystic ovary syndrome patients.

Reprod Biomed Online 2020 Oct 19;41(4):724-728. Epub 2020 May 19.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, China; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China; Shandong Provincial Clinical Medicine Research Center for Reproductive Health, Shandong University, Jinan, China. Electronic address:

Research Question: This study investigated whether rs1784692 is a risk factor for polycystic ovary syndrome (PCOS) in Han Chinese women.

Design: A case-control study was conducted in Han Chinese women, involving 526 PCOS patients and 522 control participants. A TaqMan MGB probe assay was used to genotype the variant rs1784692. Dominant and additive models were employed for genotype-phenotype association analysis in the PCOS and control samples.

Results: The minor allele C of rs1784692 is protective against PCOS (odds ratio [OR] 0.556, 95% confidence interval [CI] 0.408-0.759, P = 1.83 × 10), even after adjustment for body mass index (BMI) and age (OR 0.539, 95% CI 0.391-0.743, P= 1.62 × 10). Genotype-phenotype analysis of the dominant model showed that mean BMI in the CC+CT group was higher than in the TT group in the PCOS group (27.12 ± 5.82 versus 24.57 ± 4.52, P = 1.0 × 10), but not in the control groups, indicating that the minor allele C of rs1784692 associates with BMI in women with PCOS. The mean LH (luteinizing hormone) concentration in the CC+CT group was lower than in the TT group in PCOS and control participants (9.33 ± 5.08 versus 10.93 ± 5.91, P = 0.036; 4.39 ± 1.66 versus 4.89 ± 2.07, P = 0.021). Genotype-phenotype analysis of additive model showed that mean BMI in TC group was higher than in the TT group in PCOS patients compared with control participants (27.14 ± 5.81 versus 24.57 ± 4.52, P = 3.06 × 10).

Conclusions: The SNP rs1784692 in gene ZBTB16 is protective against PCOS but is associated with increased BMI in Han Chinese women with PCOS.
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http://dx.doi.org/10.1016/j.rbmo.2020.05.005DOI Listing
October 2020

MiR-148a-3p may contribute to flawed decidualization in recurrent implantation failure by modulating HOXC8.

J Assist Reprod Genet 2020 Oct 8;37(10):2535-2544. Epub 2020 Aug 8.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, No. 157 Jingliu Road, Jinan, 250012, Shandong, China.

Purpose: To evaluate whether miR-148a-3p overexpression is associated with disrupted decidualization of recurrent implantation failure (RIF).

Methods: Endometrial miRNA and mRNA expression profiles during the implantation window derived from women with and without RIF were identified using microarray and RT-qPCR. Immortalized human endometrial stromal cells (HESCs) were cultured for proliferation and in vitro decidualization assays after enhancing miR-148a-3p expression or inhibiting putative target gene homeobox C8 (HOXC8) expression. RT-qPCR, western blot, and luciferase reporter assays were used to confirm the relationship between miR-148a-3p and HOXC8 gene.

Results: MiR-148a-3p was significantly upregulated in RIF endometrial tissues. Forced expression of miR-148a-3p notably attenuated HESC in vitro decidualization. Mechanistic studies revealed that miR-148a-3p directly bounds to the HOXC8 3' untranslated region (3'UTR) and suppressed HOXC8 expressions in both mRNA and protein levels. Further investigations demonstrated that inhibition of HOXC8 in HESCs induced similar effects on decidual process as those induced by miR-148a-3p overexpression.

Conclusion: Taken together, our findings suggested that elevated miR-148a-3p might account for flawed decidualization in RIF by negatively regulating HOXC8, raising the possibility that miR-148a-3p might be a novel therapeutic target in RIF.
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http://dx.doi.org/10.1007/s10815-020-01900-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550445PMC
October 2020

Increased risk of metabolic dysfunction in children conceived by assisted reproductive technology.

Diabetologia 2020 10 5;63(10):2150-2157. Epub 2020 Aug 5.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, No.157 Jingliu Road, Jinan, 250000, China.

Aims/hypothesis: Assisted reproductive technology (ART) is the most widely used treatment for infertility and has resulted in millions of births worldwide. The safety of the offspring has been of the utmost concern. Previous studies suggested an increase in metabolic disorders in offspring later in life. The aim of the present study was to investigate metabolic changes at age 6-10 years in offspring conceived as a result of in vitro fertilisation/intracytoplasmic sperm injection (IVF/ICSI).

Methods: A total of 380 children born from IVF/ICSI and a matched control group of 380 naturally conceived children, all aged 6-10 years, were recruited. Anthropometric measures, ultrasound and serum tests were performed for body mass, glucose metabolism and lipid profiles, and examination of vasculature structure.

Results: The children conceived by ART showed significantly higher fasting blood glucose and serum insulin levels and HOMA-IR (adjusted β [95% CI]: fasting blood glucose 0.49 [0.42, 0.55]; log-transformed insulin 0.28 [0.20, 0.35]; log-transformed HOMA-IR 0.38 [0.30, 0.46]), as well as a lower HOMA-B and serum apolipoprotein A (ApoA) levels (adjusted β [95% CI]: log-transformed HOMA-B -0.19 [-0.27, -0.11]; ApoA -0.17 [-0.21, -0.13]), when compared with the control group. Furthermore, the ultrasound scan indicated elevated carotid intima-media thickness in children conceived by ART (β 0.13 [95% CI 0.12, 0.13]).

Conclusions/interpretation: Children conceived by IVF/ICSI have a less favourable glucose and cardiovascular metabolic profile in childhood when compared with naturally conceived children. The underlying mechanisms and potential long-term consequences need to be elucidated in future studies. Graphical abstract.
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http://dx.doi.org/10.1007/s00125-020-05241-1DOI Listing
October 2020

Electron mobility and mode analysis of scattering for β-GaO from first principles.

J Phys Condens Matter 2020 Jul 23;32(46):465704. Epub 2020 Jul 23.

School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan 430074, People's Republic of China.

The electrical transport properties of β-GaO are studied by first-principles calculations. The calculated intrinsic electron Hall mobilities agree well with experiments, with intrinsic Hall factor decreasing monotonically from 1.54 at 100 K to 1.14 at 800 K. The anisotropy of electron mobility is weak due to the almost isotropic electron effective mass, which also results in nearly isotropic Seebeck coefficient and electronic contribution to the thermal conductivity. The mode analysis of phonon scattering reveals that the optical phonon scattering is almost entirely determined by the long-range polar interactions, whereas the acoustic phonon scattering also plays an important role especially at low temperatures. The intrinsic electron mobility is significantly overestimated even above room temperature by only considering the polar optical phonon scattering, in contrast to previous predictions from fitting of phenomenological models.
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http://dx.doi.org/10.1088/1361-648X/aba8caDOI Listing
July 2020

Transmission of polycystic ovary syndrome susceptibility single-nucleotide polymorphisms and their association with phenotype changes in offspring.

Hum Reprod 2020 07;35(7):1711-1718

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.

Study Question: Does the inheritance of polycystic ovary syndrome (PCOS) susceptibility single-nucleotide polymorphism affect the phenotype of offspring?

Summary Answer: Male offspring who inherit PCOS-related genetic variations from PCOS mothers were more susceptible to developing the metabolic abnormality in their later life.

What Is Known Already: Genetic factors are considered the major etiology of PCOS. Previous studies have highlighted that offspring of women with PCOS had an increased risk of the same disease or PCOS-like symptoms.

Study Design, Size, Duration: The study involved 172 children born to women with PCOS and 529 children born to non-PCOS women. All offspring were conceived by assisted reproductive technologies.

Participants/materials, Setting, Methods: The offspring ranged from 1 to 8 years old. Metabolic phenotype analyses were performed in offspring aged from 2 to 8 (N = 619). Sanger sequencing, TaqMan and Sequenom MassARRAY were used to sequence the samples.

Main Results And The Role Of Chance: In male offspring, the fasting insulin (FINS) (P = 0.037) homeostasis model assessment of insulin resistance (HOMA-IR) (P = 0.038) and the homeostasis model assessment of pancreatic beta-cell function (HOMA-β) (P = 0.038) levels were higher in offspring of PCOS mothers compared to controls. In female offspring, PCOS offspring had a significantly higher anti-Müllerian hormone levels (P = 0.001) compared to those from control mothers. In male offspring of PCOS mothers, subjects with a T allele at rs2349415 in the gene FSHR had higher FINS (P = 0.023), HOMA-IR (P = 0.030) and HOMA-β levels (P = 0.013) than those in the homozygous CC group. The same increased trend in FINS, HOMA-IR and HOMA-β levels could be found in the CC and TC group in rs2268361 located in gene FSHR compared to the TT group (P = 0.029, P = 0.030, P = 0.046, respectively). As for rs10818854 in the DENND1A gene, the AA and AG group had a higher FINS (P = 0.037) and HOMA-β (P = 0.008) levels than the homozygous CC group.

Limitations, Reasons For Caution: Firstly, the offspring may be too young to see any phenotype changes. Secondly, this study only analyzed the differences of genotype frequency using the dominant model instead of all three models due to the limited sample size of the homozygous model. The results, therefore, should be replicated and performed in a larger sample size population. Thirdly, environmental impacts cannot be ruled out.

Wider Implications Of The Findings: The findings presented in this thesis add to our understanding the changes in offspring born to PCOS women and remind us to consider early intervention to avoid more severe effects.

Study Funding/competing Interest(s): This study was supported by the National Key Research and Development Program of China 2017YFC1001000 (to Z.-J.C.), the National Natural Science Foundation of China 81430029 (to Z.-J.C.), 81622021 and 31571548 (to H.Z.), the National Natural Science Foundation of Shandong Province JQ201816 (to H.Z.) and Shandong Provincial Key Research and Development Program 2017G006036 (to L.-L.C.) and 2018YFJH0504 (to Z.-J.C.). There are no conflicts of interest to declare.

Trial Registration Number: N/A.
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http://dx.doi.org/10.1093/humrep/deaa125DOI Listing
July 2020
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