Publications by authors named "Jinjin Liu"

84 Publications

Ultrasound-based radiomics score: a potential biomarker for the prediction of progression-free survival in ovarian epithelial cancer.

Abdom Radiol (NY) 2021 Jun 13. Epub 2021 Jun 13.

Department of Ultrasound Imaging, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

Purpose: More than 80% of patients with ovarian epithelial cancer (OEC) show complete remission after initial treatment but eventually experience recurrence of the disease. This study aimed to develop a radiomics signature to identify a new prognostic indicator based on preoperative ultrasound imaging.

Methods: A total of 111 patients with OEC who underwent transvaginal ultrasound before surgery were included. Of these, 76 were divided into the training cohort and 35 into the test cohort. We defined the region of interest (ROI) of the tumor by manually drawing the tumor contour on the ultrasound image of the lesion. The radiomics features were extracted from ultrasound images. The radiomics score (Rad-Score) was constructed using the least absolute shrinkage and selection operator (LASSO) analysis and Cox regression. Combined with the ultrasound radiomics features, significant clinical variables were also used to establish predictive models for 5-year progression-free survival (PFS) prediction. The efficiency of the model was evaluated using the area under the curve (AUC). Kaplan-Meier analysis was used to evaluate the association between the Rad-Score and PFS.

Results: The combined model was superior to the clinical and Rad-Score models in estimating 5-year PFS and achieved an AUC of 0.868 (95%CI 0.766-0.971) in the training cohort. The Rad-Score was negatively correlated with prognosis in the training and test cohorts.

Conclusions: The combined model that incorporated both clinical parameters and ultrasound radiomics features achieved a good prognosis in patients with OEC, which might aid clinical decision-making.
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http://dx.doi.org/10.1007/s00261-021-03163-zDOI Listing
June 2021

Electrochemical aptasensor for 17β-estradiol using disposable laser scribed graphene electrodes.

Biosens Bioelectron 2021 Aug 28;185:113247. Epub 2021 Apr 28.

Polymer Biointerface Centre, School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of Wellington, PO Box 600, Wellington, New Zealand. Electronic address:

17β-Estradiol (E2), the strongest of the three major physiological estrogens in females, is an important factor in the female reproductive system. The abnormal level of E2 causes health issues, such as weak bones, urinary tract infections and even depression. Here, we present a novel, sensitive and selective, electrochemical aptasensor for detection of 17β-estradiol (E2). The E2 recognition aptamer was split into two fragments: the first fragment, functionalised with adamantane, is attached to poly(β-cyclodextrin) (poly(β-CD))-modified electrode surface through host-guest interactions between the adamantane and poly(β-CD). The second fragment, labelled with gold nanoparticles, forms the stem-loop structure with the first fragment only in the presence of E2. That specific recognition process triggers the change in the electrochemical signal (a change in the peak current from reduction of AuNPs), recorded by means of differential pulse voltammetry (DPV). The feasibility of the sensing design was firstly investigated on the commercially available glass carbon electrodes (GCE), with achieved a linear detection range of 1.0 × 10 to 1.0 × 10 M and a limit of detection (LoD) 0.7 fM. The sensing methodology was then translated onto single-use, disposable, laser-scribed graphene electrodes (LSGE) on a plastic substrate. The dynamic sensing range of E2 on LSGE was found to be 1.0 × 10 to 1.0 × 10 M, with a LoD of 63.1 fM, comparable to these of GCE. The successful translation of the developed E2 aptasensor from GCE to low-cost, disposable LSGE highlights a potential of this sensing platform in commercial, portable sensing detection systems for E2 and similar targets of biological interest.
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http://dx.doi.org/10.1016/j.bios.2021.113247DOI Listing
August 2021

Coronal Compensation Mechanism of Pelvic Obliquity in Patients With Developmental Dysplasia of the Hip.

Global Spine J 2021 May 5:21925682211010760. Epub 2021 May 5.

NanKai University School of Medicine, Tianjin, China.

Study Design: Descriptive retrospective study.

Objective: DDH is a common lower limb deformity. It has been reported that this deformity can change the sagittal alignment of spine and pelvis. Sacral obliquity (SO) and iliac obliquity (IO) have been proven simple and reliable pelvic-lower limb coronal parameters to evaluate the coronal balance of the pelvis. To study the spine-pelvic coronal compensation mechanism of pelvic obliquity in patients with developmental dysplasia of the hip (DDH).

Methods: We collected the data of 110 patients with DDH in our hospital from January 2009 to December 2019, who underwent total hip arthroplasty. We used ICC (Intraclass correlation coefficient) to test the reliability of the measurement result of SO and IO in patients with DDH. A paired t-test was used for the statistical analysis.

Results: The intra observer reliability of IO and SO was 0.965 and 0.875 respectively, and the inter observer reliability of IO and SO was 0.887 and 0.889 respectively. The sacroiliac joint and spine can compensate for pelvic obliquity in patients with DDH. We classified the compensatory mechanism into 3 types according to the different imaging performances.

Conclusions: The sacroiliac joint has a considerable compensatory ability to ensure coronal balance. We clarified and classified the compensatory mechanism of pelvic obliquity into 3 types. We differentiated the pelvic obliquity in patients with DDH and in those with scoliosis, which is clinical significant to understand the process of development of the disease.
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http://dx.doi.org/10.1177/21925682211010760DOI Listing
May 2021

A nomogram to predict rupture risk of middle cerebral artery aneurysm.

Neurol Sci 2021 Apr 15. Epub 2021 Apr 15.

Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.

Background: Determining the rupture risk of unruptured intracranial aneurysm is crucial for treatment strategy. The purpose of this study was to predict the rupture risk of middle cerebral artery (MCA) aneurysms using a machine learning technique.

Methods: We retrospectively reviewed 403 MCA aneurysms and randomly partitioned them into the training and testing datasets with a ratio of 8:2. A generalized linear model with logit link was developed using training dataset to predict the aneurysm rupture risk based on the clinical variables and morphological features manually measured from computed tomography angiography. To facilitate the clinical application, we further constructed an easy-to-use nomogram based on the developed model.

Results: Ruptured MCA aneurysm had larger aneurysm size, aneurysm height, perpendicular height, aspect ratio, size ratio, bottleneck factor, and height-width ratio. Presence of a daughter-sac was more common in ruptured than in unruptured MCA aneurysms. Six features, including aneurysm multiplicity, lobulations, size ratio, bottleneck factor, height-width ratio, and aneurysm angle, were adopted in the model after feature selection. The model achieved a relatively good performance with areas under the receiver operating characteristic curves of 0.77 in the training dataset and 0.76 in the testing dataset. The nomogram provided a visual interpretation of our model, and the rupture risk probability of MCA aneurysms can be directly read from it.

Conclusion: Our model can be used to predict the rupture risk of MCA aneurysm.
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http://dx.doi.org/10.1007/s10072-021-05255-6DOI Listing
April 2021

Rapid initiation and stable maintenance of municipal wastewater nitritation during the continuous flow anaerobic/oxic process with an ultra-low sludge retention time.

Water Res 2021 Jun 29;197:117091. Epub 2021 Mar 29.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address:

Rapid achievement of nitritation of mainstream municipal wastewater in a continuous-flow process is attractive since it favors the involvement of the anammox process and reduces the operational costs. In this study, a feasible and economical strategy is proposed to rapidly achieve the nitritation of municipal wastewater. By aggressively discharging excess sludge during the seasonal warming period (temperature increasing from 18°C to 22°C), nitritation was established in 15 days with a nitrite accumulation ratio of 85.09% in a continuous-flow anaerobic/oxic (An/O) reactor. Meanwhile, qPCR results revealed that amoA abundance increased from (1.78±0.10) × 10 copies/(g VSS) to (1.05±0.11) × 10 copies/(g VSS) while the abundance of nitrite-oxidizing bacteria decreased from (1.1±0.02) × 10 copies/(g VSS) to (5.01±0.02) × 10 copies/(g VSS). The temperature gradually stabilized at 26°C during the following operational period and stable nitritation was maintained with a nitrite accumulation ratio above 90%, which was mainly attributed to a short sludge retention time (SRT) of 4.3 days and a low dissolved oxygen of 0.86 ± 0.5 mg/L. Falling temperature negatively impacted the stability of nitritation, but nitritation could be restarted by aggressively discharging excess sludge during another temperature increase period. Overall, this study provides a feasible strategy to start-up nitritation that has great potential applications for municipal wastewater treatment.
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http://dx.doi.org/10.1016/j.watres.2021.117091DOI Listing
June 2021

Arc concave sign on thin-section computed tomography:A novel predictor for invasive pulmonary adenocarcinoma in pure ground-glass nodules.

Eur J Radiol 2021 Jun 27;139:109683. Epub 2021 Mar 27.

Depatment of Radiology, The First Affiliated Hospital of Wenzhou Medical University, NO.2 Fuxue Rd, Wenzhou, 325000, China. Electronic address:

Objective: We aimed to investigate the risk factors of invasive pulmonary adenocarcinoma, especially to report and validate the use of our newly identified arc concave sign in predicting invasiveness of pure ground-glass nodules (pGGNs).

Methods: From January 2015 to August 2018, we retrospectively enrolled 302 patients with 306 pGGNs ≤ 20 mm pathologically confirmed (141 preinvasive lesions and 165 invasive lesions). Arc concave sign was defined as smooth and sunken part of the edge of the lesion on thin-section computed tomography (TSCT). The degree of arc concave sign was expressed by the arc chord distance to chord length ratio (AC-R); deep arc concave sign was defined as AC-R larger than the optimal cut-off value. Logistic regression analysis was used to identify the independent risk factors of invasiveness.

Results: Arc concave sign was observed in 65 of 306 pGGNs (21.2 %), and deep arc concave sign (AC-R > 0.25) were more common in invasive lesions (P = 0.008). Under microscope, interlobular septal displacements were found at tumour surface. Multivariate analysis indicated that irregular shape (OR, 3.558; CI: 1.374-9.214), presence of deep arc concave sign (OR, 3.336; CI: 1.013-10.986), the largest diameter > 10.1 mm (OR, 4.607; CI: 2.584-8.212) and maximum density > -502 HU (OR, 6.301; CI: 3.562-11.148) were significant independent risk factors of invasive lesions.

Conclusions: Arc concave sign on TSCT is caused by interlobular septal displacement. The degree of arc concave sign can reflect the invasiveness of pGGNs. Invasive lesions can be effectively distinguished from preinvasive lesions by the presence of deep arc concave sign, irregular shape, the largest diameter > 10.1 mm and maximum density > -502 HU in pGGNs ≤ 20 mm.
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http://dx.doi.org/10.1016/j.ejrad.2021.109683DOI Listing
June 2021

Green synthesis of olefin-linked covalent organic frameworks for hydrogen fuel cell applications.

Nat Commun 2021 Mar 31;12(1):1982. Epub 2021 Mar 31.

State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin, China.

Green synthesis of crystalline porous materials for energy-related applications is of great significance but very challenging. Here, we create a green strategy to fabricate a highly crystalline olefin-linked pyrazine-based covalent organic framework (COF) with high robustness and porosity under solvent-free conditions. The abundant nitrogen sites, high hydrophilicity, and well-defined one-dimensional nanochannels make the resulting COF an ideal platform to confine and stabilize the HPO network in the pores through hydrogen-bonding interactions. The resulting material exhibits low activation energy (E) of 0.06 eV, and ultrahigh proton conductivity across a wide relative humidity (10-90 %) and temperature range (25-80 °C). A realistic proton exchange membrane fuel cell using the olefin-linked COF as the solid electrolyte achieve a maximum power of 135 mW cm and a current density of 676 mA cm, which exceeds all reported COF materials.
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http://dx.doi.org/10.1038/s41467-021-22288-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012354PMC
March 2021

Investigation of the Active Ingredients and Mechanism of in Asthma Based on Network Pharmacology and Experimental Verification.

Drug Des Devel Ther 2021 9;15:1075-1089. Epub 2021 Mar 9.

Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, People's Republic of China.

Background: is a Chinese medicine commonly used to treat phlegm-heat asthma. However, its anti-asthmatic active ingredients and mechanism are still unknown. The aim of this study was to predict the active ingredients and pathways of and to further explore the potential molecular mechanism in asthma by using network pharmacology.

Methods: The active ingredients and their targets related to were seeked out with the TCM systematic pharmacology analysis platform (TCMSP), and the ingredient-target network was constructed. The GeneCards, DrugBank and OMIM databases were used to collect and screen asthma targets, and then the drug-target-disease interaction network was constructed with Cytoscape software. A target protein-protein interaction (PPI) network was constructed using the STRING database to screen key targets. Finally, GO and KEGG analyses were used to identify biological processes and signaling pathways. The anti-asthmatic effects of and its active ingredients were tested in vitro for regulating airway smooth muscle (ASM) cells proliferation and MUC5AC expression, two main symptoms of asthma, by using Real-time PCR, Western blotting, CCK-8 assays and annexin V-FITC staining.

Results: Twelve active ingredients in and 479 related target proteins were screened in the relevant databases. Among these target proteins, 191 genes had been found to be differentially expressed in asthma. PPI network analysis and KEGG pathway enrichment analysis predicted that the could regulate the AKT, MAPK and apoptosis signaling pathways. Consistently, further in vitro experiments demonstrated that and resveratrol (one active ingredient of ) were shown to inhibit ASM cells proliferation and promoted apoptosis of ASM cells. Furthermore, and resveratrol inhibited PDGF-induced AKT/mTOR activation in ASM cells. In addition, Polygonum cuspidatum decreased HO induced MUC5AC overexpression in airway epithelial NCI-H292 cells.

Conclusion: Polygonum cuspidatum could alleviate the symptoms of asthma including ASM cells proliferation and MUC5AC expression through the mechanisms predicted by network pharmacology, which provides a basis for further understanding of in the treatment of asthma.
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http://dx.doi.org/10.2147/DDDT.S275228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955765PMC
March 2021

Radiomics in Stroke Neuroimaging: Techniques, Applications, and Challenges.

Aging Dis 2021 Feb 1;12(1):143-154. Epub 2021 Feb 1.

Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang, China.

Stroke is a leading cause of disability and mortality worldwide, resulting in substantial economic costs for post-stroke care each year. Neuroimaging, such as cranial computed tomography or magnetic resonance imaging, is the backbone of stroke management strategies, which can guide treatment decision-making (thrombolysis or hemostasis) at an early stage. With advances in computational technologies, particularly in machine learning, visual image information can now be converted into numerous quantitative features in an objective, repeatable, and high-throughput manner, in a process known as radiomics. Radiomics is mainly used in the field of oncology, which remains an area of active research. Over the past few years, investigators have attempted to apply radiomics to stroke in the hope of gaining benefits similar to those obtained in cancer management, i.e., in promoting the development of personalized precision medicine. Currently, radiomic analysis has shown promise for a variety of applications in stroke, including the diagnosis of stroke lesions, early prediction of outcomes, and evaluation for long-term prognosis. In this article, we elaborate the contributions of radiomics to stroke, as well as the subprocesses and techniques involved in radiomics studies. We also discuss the potential challenges facing its widespread implementation in routine practice and the directions for future research.
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http://dx.doi.org/10.14336/AD.2020.0421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801280PMC
February 2021

Mean apparent diffusion coefficient in a single slice may predict tumor response to whole-brain radiation therapy in non-small-cell lung cancer patients with brain metastases.

Eur Radiol 2021 Jan 16. Epub 2021 Jan 16.

Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, South Baixiang Street, Wenzhou, 325000, People's Republic of China.

Objectives: This study aimed to access the performance of apparent diffusion coefficient (ADC) as a predictor for treatment response to whole-brain radiotherapy (WBRT) in patients with brain metastases (BMs) from non-small-cell lung cancer (NSCLC).

Methods: A retrospective analysis was conducted of 102 NSCLC patients with BMs who underwent WBRT between 2012 and 2016. Diffusion-weighted MRI were performed pre-WBRT and within 12 weeks after WBRT started. Mean single-plane ADC value of ROIs was evaluated by two radiologists blinded to results of each other. The treatment response rate, intracranial progression-free survival (PFS), and overall survival (OS) were analyzed based on the ADC value and ΔADC respectively. At last, we used COX and logistic regression to do the multivariate analysis.

Results: There was good inter-observer agreement of mean ADC value pre-WBRT, post-WBRT, and ΔADC between the 2 radiologists (Pearson correlation 0.915 [pre-WBRT], 0.950 [post-WBRT], 0.937 [ΔADC], p < 0.001, for each one). High mean ADC value were related with better response rate (72.2% vs 37.5%, p = 0.001) and iPFS (7.6 vs 6.4 months, p = 0.031). High ΔADC were related with better response rate (73.6% vs 36.7%, p < 0.001). Multivariate analysis shows that histopathology, BMs number, high ADC value pre-WBRT, and high ΔADC post-WBRT were related to better treatment response of WBRT, and KPS, BMs number, and low ADC value pre-WBRT increased the risk of developing intracranial relapse.

Conclusions: The mean single-plane ADC value pre-WBRT and ΔADC post-WBRT were potential predictor for intracranial tumor response to WBRT in NSCLC patients with brain metastases.

Key Points: • ADC value is a potential predictor of intracranial treatment response to WBRT in NSCLC patients with brain metastases. • Higher mean ADC value pre-WBRT and ΔADC post-WBRT of brain metastases were related to better intracranial tumor response. • Prediction of response before WBRT using ADC value can help oncologists to make better therapy plans and avoid missing opportunities for rescue therapy.
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http://dx.doi.org/10.1007/s00330-020-07584-0DOI Listing
January 2021

Benzo(a)pyrene induces MUC5AC expression through the AhR/mitochondrial ROS/ERK pathway in airway epithelial cells.

Ecotoxicol Environ Saf 2021 Mar 8;210:111857. Epub 2021 Jan 8.

Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, PR China. Electronic address:

Objectives: Benzo(a)pyrene (BaP) is a ubiquitous air pollutants, and BaP exposure leads to a risk of respiratory diseases. The oversecretion of airway mucus and high expression of mucin 5AC (MUC5AC) are associated with common respiratory disorders caused by air pollution. We aimed to investigate the effect of BaP on MUC5AC expression, especially the mechanisms by which BaP induces MUC5AC gene expression.

Methods: The human airway epithelial cell NCI-H292 was used to test the effects of BaP on the expression of MUC5AC in vitro. MUC5AC mRNA and protein expression were assessed with real-time quantitative PCR, immunochemistry, and western blotting. A luciferase assay was conducted to detect the activity of the promoter. The total cellular ROS and mitochondrial ROS were measured by corresponding probes. Small-interfering RNAs were used for gene silencing. AhR-overexpressing cell lines were constructed by transfection with AhR overexpression lentivirus.

Results: We found that BaP stimulation upregulated the MUC5AC mRNA and protein levels and activated the ERK pathway. Suppressing ERK with U0126 (an ERK inhibitor) or knocking down ERK with siRNA decreased BaP-induced MUC5AC expression. The luciferase activity transfected with the MUC5AC promoter and cAMP-response element (CRE) was increased after BaP treatment, whereas CREB siRNA suppressed the BaP-induced overexpression of MUC5AC. In addition, BaP increased mitochondrial ROS production, and Mito-TEMP, a mitochondrial ROS inhibitor, inhibited BaP-induced MUC5AC expression and ERK activation. BaP increased the mRNA levels of CYP1A1 and CYP1B1, while Alizarin, a CYP1s inhibitor, suppressed the effects of BaP, including the MUC5AC overexpression, ERK activation and mitochondrial ROS generation. BaP induced the translocation of aryl hydrocarbon receptor (AhR) from the cytoplasm to the nucleus. SiRNA-mediated knockdown or chemical inhibition of AhR decreased the BaP-induced expression of MUC5AC, while the overexpression of AhR significantly enhanced the BaP-induced expression of MUC5AC. ITE, an endogenous AhR ligand, also upregulated the mRNA and protein expression of MUC5AC. Furthermore, resveratrol treatment inhibited the BaP-induced MUC5AC overexpression, AhR translocation, mitochondrial ROS production and ERK pathway activation.

Conclusion: Here, we highlighted the crucial role of AhR/mitochondrial ROS/ERK pathway activation in BaP-induced MUC5AC overexpression and identified resveratrol as a promising drug to reduce BaP-induced MUC5AC overexpression.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111857DOI Listing
March 2021

Correlation of serum vitamin D with lipid profiles in middle-aged and elderly Chinese individuals.

Asia Pac J Clin Nutr 2020 ;29(4):839-845

Department of Endocrinology, the First Hospital of Lanzhou University, Lanzhou, China. Email:

Background And Objectives: Deficiency of vitamin D has been associated with various health conditions. The purpose of this study was to evaluate the associations between the serum 25OHD concentration and lipid profiles in Chinese individuals.

Methods And Study Design: Serum 25OHD and lipid profiles were obtained for a cross sectional sample of 10100 individuals aged 40-75 years from Lanzhou city, which is located in western China. Linear-by-linear association, partial correlation analysis and multiple logistic regression analysis were used to evaluate associations between serum 25OHD concentration and lipid profiles.

Results: 10038 subjects aged 40- 75 years were included in the study. The 25OHD deficient and insufficient groups had higher TC, LDL-C and TG when compared to the optimal group. The dyslipidemia rates of vitamin D deficiency, insufficiency, and sufficiency groups were 45.4%, 41.6%, 38.8%, respectively. The prevalence rates of dyslipidemia, high cholesterol, high LDL-C, hypertriglyceridemia and mixed type hyperlipidemia exhibited decline trend in vitamin D deficiency, insufficiency and sufficiency groups. The correlation coefficients in between TC and 25OHD, LDL-C and 25OHD, TG and 25OHD were -0.033, -0.022, -0.044, respectively. Low 25OHD levels were associated with the risk of onset of dyslipidemia [OR 1.225 (95% CI 1.075-1.397), p=0.002] in the logistical regression analyses.

Conclusions: Deficient serum 25OHD is associated with higher TC, LDL-C, and TG in middle-aged and elderly Chinese individuals. These findings suggest that low 25OHD levels observationally is simply a marker for elevated atherogenic lipoproteins and question a role for vitamin D supplementation in the prevention of cardiovascular disease.
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http://dx.doi.org/10.6133/apjcn.202012_29(4).0020DOI Listing
January 2020

In Reply: Predicting Long-Term Outcomes After Poor-Grade Aneurysmal Subarachnoid Hemorrhage Using Decision Tree Modeling.

Neurosurgery 2021 02;88(3):E300-E301

Department of Radiology The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.

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http://dx.doi.org/10.1093/neuros/nyaa521DOI Listing
February 2021

Different functional connectivity modes of the right fronto-insular cortex in akinetic-rigid and tremor-dominant Parkinson's disease.

Neurol Sci 2020 Nov 24. Epub 2020 Nov 24.

Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.

Background: Patients with akinetic-rigid Parkinson's disease (AR-PD) are more prone to cognitive decline and depressive symptoms than tremor-dominant PD (TD-PD) patients. The right fronto-insular cortex (rFIC), as a key node of salience network, plays a critical role in the switching between central executive network and default mode network. In this study, we explored the functional connectivity mode of rFIC with triple-brain networks, namely default mode network, salience network, and central executive network, in two motor subtypes of PD.

Methods: We recruited 44 PD patients (including the TD-PD group and AR-PD group) and 18 age-matched healthy controls (HCs). We performed functional connectivity (FC) analysis of resting-state functional MRI.

Results: Compared with TD-PD, decreased FC were found in the right insular cortex and bilateral anterior cingulate gyrus in AR-PD. Compared with HCs, decreased FC in the bilateral insula, the anterior cingulate gyrus, the precentral gyrus, and the right medial frontal gyrus were found; therein, the FC value of rFIC-precentral gyrus was positively correlated with the Unified Parkinson's Disease Rating Scale-II score in AR-PD (p = 0.0482, r = 0.4162). While TD-PD showed decreased FC in the left insula as well as bilateral anterior cingulate gyrus when compared with HCs, and the FC value of the rFIC-left insula was positively correlated with its Hamilton Depression Rating Scale score (p = 0.02, r = 0.50).

Conclusion: The functional connectivity mode of rFIC in AR-PD differed from that in TD-PD. The decreased rFIC FC with the other nodes of salience network might be a potential indicator for AR-PD patients prone to develop cognitive decline and depressive symptoms.
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http://dx.doi.org/10.1007/s10072-020-04917-1DOI Listing
November 2020

Prediction of Clinical Outcome at Discharge After Rupture of Anterior Communicating Artery Aneurysm Using the Random Forest Technique.

Front Neurol 2020 29;11:538052. Epub 2020 Oct 29.

Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Aneurysmal subarachnoid hemorrhage (SAH) is a devastating disease. Anterior communicating artery (ACoA) aneurysm is the most frequent location of intracranial aneurysms. The purpose of this study is to predict the clinical outcome at discharge after rupture of ACoA aneurysms using the random forest machine learning technique. A total of 607 patients with ruptured ACoA aneurysms were included in this study between December 2007 and January 2016. In addition to basic clinical variables, 12 aneurysm morphologic parameters were evaluated. A multivariate logistic regression analysis was performed to determine the independent predictors of poor outcome. Of the 607 patients, 485 patients were randomly selected for training and the remaining for internal testing. The random forest model was developed using the training data set. An additional 202 patients from February 2016 to December 2017 were collected for externally validating the model. The prediction performance of the random forest model was compared with two radiologists. Patients' age (odds ratio [OR] = 1.04), ventilated breathing status (OR = 4.23), World Federation of Neurosurgical Societies (WFNS) grade (OR = 2.13), and Fisher grade (OR = 1.50) are significantly associated with poor outcome. None of the investigated morphological parameters of ACoA aneurysm is an independent predictor of poor outcome. The developed random forest model achieves sensitivities of 78.3% for internal test and 73.8% for external test. The areas under receiver operating characteristic (ROC) curve of the random forest model were 0.90 for the internal test and 0.84 for the external test. Both sensitivities and areas under ROC curves of our model are superior to those of two raters in both internal and external tests. The random forest model presents good performance in predicting the outcome after rupture of ACoA aneurysms, which may aid in clinical decision making.
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http://dx.doi.org/10.3389/fneur.2020.538052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658443PMC
October 2020

Genomic and phylodynamic analysis of sapoviruses isolated in Henan Province, China.

Arch Virol 2021 Jan 9;166(1):265-270. Epub 2020 Nov 9.

The Sixth People's Hospital of Zhengzhou, Zhengzhou, 450000, People's Republic of China.

In this study, we determined the near-complete and partial genome sequences of ten SaV isolates. Phylogenetic analysis based on full-length VP1 and RdRp nucleotide sequences indicated that nine isolates were of GI.1 and one was GII.3. Evolutionary dynamics analysis indicated that GI.1 and GII.3 SaVs evolved at different rates, the latter evolving more rapidly. Cluster analysis indicated that distantly related GI.1 SaVs were more similar in their amino acid compositions than were GII.3 SaVs. The data provided in this study may facilitate studies on SaV genomic diversity and epidemiological patterns in China and worldwide.
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http://dx.doi.org/10.1007/s00705-020-04876-0DOI Listing
January 2021

Risk Factors for The Growth of Residual Nodule in Surgical Patients with Adenocarcinoma Presenting as Multifocal Ground-glass Nodules.

Eur J Radiol 2020 Dec 21;133:109332. Epub 2020 Oct 21.

Depatment of Radiology, Wenzhou Medical University, First Affiliated Hospital, NO. 2 Fuxue Rd., Wenzhou, 325000, China. Electronic address:

Purpose: We aim to investigate the risk factors influencing the growth of residual nodule (RN) in surgical patients with adenocarcinoma presenting as multifocal ground-glass nodules (GGNs).

Method: From January 2014 to June 2018, we enrolled 238 patients with multiple GGNs in a retrospective review. Patients were categorized into growth group 63 (26.5%), and non-growth group 175 (73.5%). The median follow-up time was 28.2 months (range, 6.3-73.0 months). To obtain the time of RN growth and find the risk factors for growth, data such as age, gender, history of smoking, history of malignancy, type of surgery, pathology and radiological characteristics were analyzed to use Kaplan-Meier method with the log-rank test and Cox regression analysis.

Results: The median growth time of RN was 56.0 months (95% CI, 45.0-67.0 months) in all 238 patients. Roundness (HR 4.62, 95% CI 2.20-9.68), part-solid nodule (CTR ≥ 50%) (HR 4.39, 95% CI 2.29-8.45), vascular convergence sign (HR 2.32, 95% CI 1.36-3.96) of RN, and age (HR 1.04, 95% CI 1.01-1.07) were independent predictors of further nodule growth. However, radiological characteristics and pathology of domain tumour (DT) cannot be used as indicators to predict RN growth.

Conclusions: RN showed an indolent growth pattern in surgical patients with multifocal GGNs. RN with a higher roundness, presence of vascular convergence sign, more solid component, and in the elder was likely to grow. However, the growth of RN showed no association with the radiological features and pathology of DT.
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http://dx.doi.org/10.1016/j.ejrad.2020.109332DOI Listing
December 2020

[Correlation of type 2 diabetes and impaired glucose regulation with chronic kidney disease in middle-aged and elderly individuals].

Nan Fang Yi Ke Da Xue Xue Bao 2020 Oct;40(10):1457-1464

Department of Endocrinology, First Hospital of Lanzhou University, Gansu Provincial People's Hospital, Lanzhou 730000, China.

Objective: To explore the correlation of different glucose metabolism statues with chronic kidney disease (CKD) in middle-aged and elderly individuals in Lanzhou.

Methods: Based on the baseline data of REACTION Study in Lanzhou area, we randomly sampled 10 038 residents aged 40-75 years in 3 communities in Lanzhou, who were classified into normal glucose tolerance (NGT), impaired glucose regulation (IGR) and diabetes groups. The estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatinine ratio (ACR) were used to assess the renal function and albuminuria, respectively. Binary logistic regression was performed to analyze the contribution of the risk factors to CKD. Polynominal regression was used to determine the trends of eGFR with the increment of ACR.

Results: Among all the participants, the prevalences of albuminuria, CKD and renal insufficiency (RI) were 26.2%, 27.4% and 2.5%, respectively. The prevalence of albuminuria, CKD and RI were significantly higher in the diabetes group than in IGR and NGT groups ( < 0.05). In IGR group, age, hypertension, and hypertriglyceridemia were positively correlated with the risk of RI (OR: 1.113, 1.904, and 2.608, respectively; < 0.05). In diabetes group, age, coronary heart disease, obesity, hypertriglyceridemia, and elevated LDL-C level were positively correlated with the risk of RI (OR: 1.069, 2.535, 3.359, 1.827, and 2.690, respectively; < 0.05). Logistic regression analysis showed that diabetes mellitus significantly increased the risk of albuminuria (OR: 1.543, =0.000) and RI (OR: 1.446, =0.005). Logistic regression analysis and multivariate regression analysis showed that although the deterioration trends of eGFR were similar in diabetes group and IGR group, IGR was not a significant risk factor for albuminuria or RI (OR:1.057, =0.355; OR: 0.918, =0.614).

Conclusions: Diabetes mellitus is a significant risk factor for albuminuria and RI, while IGR is not. Screening for albuminuria and eGFR is highly recommended for individuals with diabetes, hypertension, and obesity, especially in women and the elderly population.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2020.10.11DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606229PMC
October 2020

Tethering Flexible Polymers to Crystalline Porous Materials: A Win-Win Hybridization Approach.

Angew Chem Int Ed Engl 2021 Jun 25;60(26):14222-14235. Epub 2021 Feb 25.

Renewable energy conversion and storage center, College of Chemistry, Nankai University, Tianjin, 300071, China.

Crystalline porous materials (CPMs) have been widely studied over the past few decades due to their well-defined, customizable, and porous structures. However, the limited stability and machinability restrict their particle applications. Recently, a new trend to use hybrid strategies to bridge the gap between CPMs and flexible polymers has emerged as an effective solution to tackle the above issues. Polymers can be either cross-linked within the framework or anchored to the outer surface of the materials, that endow CPMs with polymer features, such as flexibility, ductility, and machinability. Such resulting polymer-tethered CPMs (named as polyCPMs) have demonstrated improved performances in many application scopes such as membrane separation, heterogeneous catalysis and smart response. This minireview highlights state of the art for the hybridization strategies of CPMs with flexible polymers, and provide a perspective on their potential applications and future directions.
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http://dx.doi.org/10.1002/anie.202011213DOI Listing
June 2021

The Tat system and its dependent cell division proteins are critical for virulence of extra-intestinal pathogenic .

Virulence 2020 12;11(1):1279-1292

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan, China.

The twin-arginine translocation (Tat) system is involved in a variety of important bacterial physiological processes. Conserved among bacteria and crucial for virulence, the Tat system is deemed as a promising anti-microbial drug target. However, the mechanism of how the Tat system functions in bacterial pathogenesis has not been fully understood. In this study, we showed that the Tat system was critical for the virulence of an extra-intestinal pathogenic (ExPEC) strain PCN033. A total of 20 Tat-related mutant strains were constructed, and competitive infection assays were performed to evaluate the relative virulence of these mutants. The results demonstrated that several Tat substrate mutants, including the Δ, ΔΔ double mutant as well as each single mutant, Δ, Δ, and Δ, were significantly outcompeted by the WT strain, among which the Δ and ΔΔ strains showed the lowest competitive index (CI) value. Results of individual mouse infection assay, cell adhesion assay, whole blood bactericidal assay, and serum bactericidal assay further confirmed the virulence attenuation phenotype of the Δ and ΔΔ strains. Moreover, the two mutants displayed chained morphology in the log phase resembling the Δ and were defective in stress response. Our results suggest that the Tat system and its dependent cell division proteins SufI, AmiA, and AmiC play critical roles during ExPEC pathogenesis.
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http://dx.doi.org/10.1080/21505594.2020.1817709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549933PMC
December 2020

Phylogenetic and biological characterizations of a GI.3 norovirus.

Infect Genet Evol 2020 11 11;85:104554. Epub 2020 Sep 11.

The Sixth People's Hospital of Zhengzhou, Zhengzhou, China. Electronic address:

Noroviruses (NoVs) are a major cause of acute non-bacterial gastroenteritis worldwide. In this study, we report the isolation, near-complete genome sequencing, and expression and biological characterization of the major capsid protein (VP1) of a GI.3 NoV isolated from a child presenting acute gastroenteritis. The genome of the GI.3 NoV is 7746 bp in length, not including the poly-adenylation tail. Phylogenetic analysis based on the complete VP1 nucleotide sequences indicates that GI.3 NoVs could be divided into four clusters, with 4.6%, 5.3%, 6.6%, 1.9% intracluster variations in nucleotide and 4.8%, 3.8%, 6.1%, 1.7% intracluster variations in amino acid sequences, respectively. A Bayesian evolutionary analysis showed that GI.3 NoVs evolved at 2.44 × 10, 2.78 × 10, and 3.04 × 10 nucleotide substitutions/site/year using a strict clock model, an uncorrelated log-normal model (UCLN), and an uncorrelated exponential derivation model (UCED), respectively. VP1 protein expression using a recombinant baculovirus expression system leads to the successful assembly of virus-like particles (VLPs). In vitro VLP-Histo-blood group antigen (HBGA) binding assay indicates that GI.3 NoV VLPs strongly bind to blood type A salivary HBGAs, moderately bind to blood type O salivary HBGAs, and weakly bind or do not bind to blood type B and AB salivary HBGAs. In vitro VLP-HBGA binding blockade assay indicated that the binding of GI.3 NoV VLPs to blood type A salivary HBGAs could only be blocked by anti-GI.3 NoV VLPs serum but not non-GI.3 NoV genotype-specific hyperimmune sera (GI.2, GI.7, GII.4, GII.6, GII.7, and GII.17). The detailed characterization of GI.3 NoV in this study provides evidence that GI.3 NoV undergoes rapid evolution and exhibits no cross-blocking effects, suggesting that GI.3 NoV may potentially be utilized in the development of multivalent NoV vaccines.
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http://dx.doi.org/10.1016/j.meegid.2020.104554DOI Listing
November 2020

Qi-Xian Decoction Upregulated E-cadherin Expression in Human Lung Epithelial Cells and Ovalbumin-Challenged Mice by Inhibiting Reactive Oxygen Species-Mediated Extracellular-Signal-Regulated Kinase (ERK) Activation.

Med Sci Monit 2020 Aug 24;26:e922003. Epub 2020 Aug 24.

Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China (mainland).

BACKGROUND Loss of the epithelial barrier is characterized by a reduction in E-cadherin expression and is a hallmark of asthma. Qi-xian decoction (QXT) is a Chinese medicinal formula that has been used to effectively treat asthma. This study aimed to investigate the effect of QXT on E-cadherin expression in human lung epithelial 16HBE cells and ovalbumin-challenged mice and to explore the underlying molecular mechanism. MATERIAL AND METHODS Ovalbumin (OVA)-induced mice were used as a model of asthma. Real-time PCR and Western blotting were utilized to examine mRNA and protein levels. Lung tissue reactive oxygen species (ROS) levels were evaluated using dichloro-dihydro-fluorescein diacetate (DCFH-DA). Serum superoxide dismutase (SOD) and the total antioxidant capacity (TAOC) were measured via enzyme-linked immunosorbent assay (ELISA)-based analyses. 16HBE cells were utilized to explore the effect of QXT or hydrogen peroxide (H₂O₂) on the expression of E-cadherin in vitro. RESULTS We found that QXT treatment increased E-cadherin expression and decreased extracellular-signal-regulated kinase (ERK) phosphorylation levels in the lung tissues of OVA-challenged mice. QXT also downregulated ROS levels and increased serum SOD and TAOC levels in OVA-challenged mice. In vitro studies demonstrated that increased ROS generation induced by H₂O₂ resulted in decreased E-cadherin expression levels in 16HBE cells, which was attenuated by inhibition of ERK signaling. Moreover, the H₂O₂-induced downregulation of E-cadherin expression, increased ROS generation, and ERK activation in 16HBE cells were restored by treatment with QXT water or ethanol extract. CONCLUSIONS These data demonstrate that one mechanism by which QXT protects against asthma is to restore E-cadherin expression in vivo and in vitro by inhibiting ROS-mediated ERK activation.
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http://dx.doi.org/10.12659/MSM.922003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461650PMC
August 2020

Long non-coding RNA SNHG22 facilitates the malignant phenotypes in triple-negative breast cancer via sponging miR-324-3p and upregulating SUDS3.

Cancer Cell Int 2020 17;20:252. Epub 2020 Jun 17.

The Third Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, West Huan-Hu Rd., Ti Yuan Bei, Hexi District, Tianjin, 300060 People's Republic of China.

Background: Increasing evidence has indicated the important role of long non-coding RNAs (lncRNAs) in regulating the development and progression of cancers, including triple-negative breast cancer (TNBC). Small nucleolar RNA host gene 22 (SNHG22) is a novel lncRNA that has been identified as tumor-contributor in ovarian carcinoma. However, its function has not been explored in TNBC.

Methods: qRT-PCR was used to identify gene expression at mRNA level while western blot was utilized to analyze the protein level. Functional assays were implemented to identify changes on the proliferation, apoptosis and motility of TNBC cells under different conditions. Additionally, mechanistic assays, such as RIP assay, RNA pull down assay and luciferase reporter assay, were applied to assess relationships between molecules.

Results: SNHG22 represented a high expression level in TNBC tissues and cells. Besides, SNHG22 silencing restrained the proliferation, migration and invasion of TNBC cells. Furthermore, miR-324-3p that was lowly expressed in TNBC cells was conformed to be sponged by SNHG22. Moreover, upregulated miR-324-3p inhibited cell proliferation and motility in TNBC. Subsequently, we identified that SUDS3, a tumor-facilitator with elevated expression in TNBC, was the downstream target of SNHG22/miR-324-3p axis. Of note, miR-324-3p repression or SUDS3 overexpression could rescue the anti-tumor effect of SNHG22 silencing on the malignant phenotypes of TNBC cells.

Conclusion: LncRNA SNHG22 facilitated cell growth and motility in TNBC via sponging miR-324-3p and upregulating SUDS3, highlighting a new promising road for TNBC treatment development.
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http://dx.doi.org/10.1186/s12935-020-01321-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302359PMC
June 2020

Blend Sign Is a Strong Predictor of the Extent of Early Hematoma Expansion in Spontaneous Intracerebral Hemorrhage.

Front Neurol 2020 19;11:334. Epub 2020 May 19.

Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

It is unclear which imaging marker is optimal for predicting the extent of hematoma expansion (EHE). We aimed to compare the usefulness of the blend sign (BS) with that of other non-contrast computed tomography (NCCT) markers for predicting the EHE in patients with spontaneous intracerebral hemorrhage (sICH). Patients with sICH admitted to our Neurology Emergency Department between September 2013 and January 2019 were enrolled. The EHE was calculated as the absolute increase in hematoma volume between baseline and follow-up CT (within 72 h). The EHE was categorized into four groups: "no growth," "minimal change" (≤5.1 ml), "moderate change" (5.1-12.5 ml), and "massive change" (>12.5 ml). Univariate and multivariate analyses were performed to investigate the relationship between the NCCT markers [BS, black hole sign (BHS), satellite sign, and island sign] and the EHE. A total of 1,111 sICH patients were included (median age: 60 years; 66.5% males). Multiple linear regression analysis showed that the presence of the BS and BHS was independently associated with the EHE, after adjusting for confounders ( < 0.001 and = 0.003, respectively). The presence of the BS and BHS was positively correlated with growth category ( = 0.285 and = 0.199, both s < 0.001). The BS demonstrated a better predictive performance for the EHE than did the BHS [area under the curve (AUC): 0.67 vs. 0.57; both s < 0.001]. In patients with acute sICH, the BS showed a better performance in predicting the EHE compared with other NCCT markers. This imaging marker may help identify patients at a high risk of significant hematoma expansion and may facilitate its early management.
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http://dx.doi.org/10.3389/fneur.2020.00334DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248383PMC
May 2020

Evaluation of reliability and validity of the Patient Health Questionnaire-9 in patients with acne.

Dermatol Ther 2020 07 6;33(4):e13584. Epub 2020 Jul 6.

Department of Dermatology, The First Affiliated Hospital of WenZhou Medical University, WenZhou, China.

To evaluate the reliability and validity of the Patient Health Questionnaire-9 (PHQ-9) in patients with acne. Three hundred acne patients were enrolled from January 2019 to December 2019. PHQ-9 and Hamilton Depression Scale(HAMD) survey was conducted to evaluate their depression status. Cronbach's α coefficient and test-retest reliability after 1 week were used to analyze the reliability of PHQ-9. Factor analysis, Spearman correlation analysis, receiver operating characteristic curve (ROC curve) were used for validity analysis of the PHQ-9.The screening validity was analyzed in different subgroups. Two hundred fifty-eight patients with acne completed the questionnaire. Forty-seven were diagnosed with depressive disorder. The best cut-off point for the PHQ-9 score is 9 points, with a sensitivity of 95.7%,a specificity of 88.6% and the area under the ROC curve(AUC) of 0.973.In validity analysis, the correlation coefficient between the total score of PHQ-9 and HAMD was 0.766,the kappa value was 0.530.Factor analysis revealed two common factors (cognitive-affective factor and somatic symptom factor),which explained 65.52% of the total variances. In reliability analysis, the Cronbach's α coefficient of the PHQ-9 was 0.851,the test-retest reliability value was 0.824.The time to complete the PHQ-9 was significantly less than the time to complete HAMD (P < .001).The PHQ-9 shows good reliability and validity for the diagnosis of depression in patients with acne, and can be used for preliminary screening of depression.
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http://dx.doi.org/10.1111/dth.13584DOI Listing
July 2020

Effects of Angiotensin II Receptor Blockers and ACE (Angiotensin-Converting Enzyme) Inhibitors on Virus Infection, Inflammatory Status, and Clinical Outcomes in Patients With COVID-19 and Hypertension: A Single-Center Retrospective Study.

Hypertension 2020 07 29;76(1):51-58. Epub 2020 Apr 29.

From the Department of Geriatrics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China (G.Y., Z.T., L.P., J.L., J.C., S.H.).

With the capability of inducing elevated expression of ACE2 (angiotensin-converting enzyme 2), the cellular receptor for severe acute respiratory syndrome coronavirus 2, angiotensin II receptor blockers (ARBs) or ACE inhibitors treatment may have a controversial role in both facilitating virus infection and reducing pathogenic inflammation. We aimed to evaluate the effects of ARBs/ACE inhibitors on coronavirus disease 2019 (COVID-19) in a retrospective, single-center study. One hundred twenty-six patients with COVID-19 and preexisting hypertension at Hubei Provincial Hospital of Traditional Chinese Medicine in Wuhan from January 5 to February 22, 2020, were retrospectively allocated to ARBs/ACE inhibitors group (n=43) and non-ARBs/ACE inhibitors group (n=83) according to their antihypertensive medication. One hundred twenty-five age- and sex-matched patients with COVID-19 without hypertension were randomly selected as nonhypertension controls. In addition, the medication history of 1942 patients with hypertension that were admitted to Hubei Provincial Hospital of Traditional Chinese Medicine from November 1 to December 31, 2019, before the COVID-19 outbreak were also reviewed for external comparison. Epidemiological, demographic, clinical, and laboratory data were collected, analyzed, and compared between these groups. The frequency of ARBs/ACE inhibitors usage in patients with hypertension with or without COVID-19 were comparable. Among patients with COVID-19 and hypertension, those received either ARBs/ACE inhibitors or non-ARBs/ACE inhibitors had comparable blood pressure. However, ARBs/ACE inhibitors group had significantly lower concentrations of hs-CRP (high-sensitivity C-reactive protein; =0.049) and PCT (procalcitonin, =0.008). Furthermore, a lower proportion of critical patients (9.3% versus 22.9%; =0.061) and a lower death rate (4.7% versus 13.3%; =0.216) were observed in ARBs/ACE inhibitors group than non-ARBs/ACE inhibitors group, although these differences failed to reach statistical significance. Our findings thus support the use of ARBs/ACE inhibitors in patients with COVID-19 and preexisting hypertension.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.15143DOI Listing
July 2020

Characterization of HIV-1 subtypes and drug resistance mutations in Henan Province, China (2017-2019).

Arch Virol 2020 Jun 11;165(6):1453-1461. Epub 2020 Apr 11.

The Sixth People's Hospital of Zhengzhou, Zhengzhou, 450000, China.

Human immunodeficiency virus type 1 (HIV-1) infection remains a severe public health problem worldwide. In this study, we investigated the distribution of HIV-1 subtypes and the prevalence of drug resistance mutations (DRMs) among patients with HIV-1 infection in Henan Province, China. HIV-1 strains in blood samples taken from inpatients and outpatients visiting the Sixth People's Hospital of Zhengzhou from August 2017 to July 2019 with a viral load (VL) greater than 1000 copies/ml were subjected to subtype and DRMs analysis. Out of a total of 769 samples, subtype and DRM data were obtained from 657 (85.43%) samples. Phylogenetic analysis based on partial pol gene sequences indicated that the most commonly found genotype was subtype B (45.51%, 299/657), followed by CRF01_AE (28.61%, 188/657), CRF07_BC (15.68%, 103/657), CRF08_BC (0.76%, 5/657), C (0.61%, 4/657), A (0.30%, 2/657), and others (8.52%, 56/657). Circulating recombinant forms (CRFs) were most commonly found in patients who were naïve to antiretroviral treatment (ART) (68.67%, 160/233). The percentage of patients with one or more major drug-resistance mutations was 50.99% (335/657), and it was 6.44% (15/233) in ART-naive patients that were primarily infected with subtype B (17.74%). Resistance mutations were most common at codons 65, 103, 106, 184, and 190 of the reverse transcriptase gene and codon 46 of the protease gene. Our study provides detailed information about the distribution of HIV-1 subtypes and the incidence of drug resistance mutations of different subtypes in ART-experienced and naïve patients. This can guide policymakers in making decisions about treatment strategies against HIV-1.
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http://dx.doi.org/10.1007/s00705-020-04606-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222071PMC
June 2020

SMYD2 suppresses APC2 expression to activate the Wnt/β-catenin pathway and promotes epithelial-mesenchymal transition in colorectal cancer.

Am J Cancer Res 2020 1;10(3):997-1011. Epub 2020 Mar 1.

Department of General Surgery, Affiliated Hospital of Xuzhou Medical University Xuzhou 221000, China.

Cancer metastasis is a significant challenge in colorectal cancer (CRC) therapy. SET and MYND domain-containing protein 2 (SMYD2) is highly expressed in multiple cancers but is rarely studied in CRC. This study aims to identify whether abnormal expression of SMYD2 is associated with cancer metastasis in CRC. In this study, we demonstrated that SMYD2 not only promoted cell proliferation but also increased the metastatic ability of CRC. The expression of adenomatous polyposis coli 2 (APC2), an inhibitor of the Wnt/β-catenin pathway, was suppressed by SMYD2 overexpression. Overexpression of SMYD2 activated the Wnt/β-catenin pathway and then induced the epithelial-mesenchymal transition (EMT) program in CRC. Mechanistically, low APC2 expression in CRC cells was due to SMYD2-mediated DNA methylation modification. This modification might require synergism with DNMT1. In summary, our study provides new insights into SMYD2-related transcriptional regulation patterns and indicates that SMYD2 could be a potential therapeutic target for CRC patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136915PMC
March 2020

Clinical-radiomics Nomogram for Risk Estimation of Early Hematoma Expansion after Acute Intracerebral Hemorrhage.

Acad Radiol 2021 03 29;28(3):307-317. Epub 2020 Mar 29.

Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China. Electronic address:

Rationale And Objectives: Noncontrast CT-based radiomics signature has shown ability for detecting hematoma expansion (HE) in spontaneous intracerebral hemorrhage (ICH). We sought to compare its predictive performance with clinical risk factors and develop a clinical-radiomics nomogram to assess the risk of early HE.

Materials And Methods: In total, 1153 patients with ICH who underwent baseline cranial CT within 6 hours and follow-up scans within 72 hours of stroke onset were enrolled, of whom 864 (75%) were assigned to the derivation cohort and 289 (25%) to the validation cohort. Based on LASSO algorithm or stepwise logistic regression analysis, three models (clinical model, radiomics model, and hybrid model) were constructed to predict HE. The Akaike information criterion (AIC) and likelihood ratio test (LRT) were used for comparing the goodness of fit of the three models, and the AUC was used to evaluate their discrimination ability for HE.

Results: The hybrid model (AIC = 681.426; χ= 128.779) was the optimal model with the lowest AIC and highest chi-square values compared to the radiomics model (AIC = 767.979; χ = 110.234) or the clinical model (AIC = 753.757; χ = 56.448). The radiomics model was superior in the prediction of HE to the clinical model in both derivation (p = 0.009) and validation (p = 0.022) cohorts. In both datasets, the clinical-radiomics nomogram showed satisfactory discrimination and calibration for detecting HE (AUC = 0.771, Sensitivity = 87.0%; AUC = 0.820, Sensitivity = 88.1%; respectively).

Conclusion: Among patients with acute ICH, noncontrast CT-based radiomics model outperformed the clinical-only model in the prediction of HE, and the established clinical-radiomics nomogram with favorable performance can offer a noninvasive tool for the risk stratification of HE.
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http://dx.doi.org/10.1016/j.acra.2020.02.021DOI Listing
March 2021

Predicting Long-Term Outcomes After Poor-Grade Aneurysmal Subarachnoid Hemorrhage Using Decision Tree Modeling.

Neurosurgery 2020 09;87(3):523-529

Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.

Background: Despite advances in the treatment of poor-grade aneurysmal subarachnoid hemorrhage (aSAH), predicting the long-term outcome of aSAH remains challenging, although essential.

Objective: To predict long-term outcomes after poor-grade aSAH using decision tree modeling.

Methods: This was a retrospective analysis of a prospective multicenter observational registry of patients with poor-grade aSAH with a World Federation of Neurosurgical Societies (WFNS) grade IV or V. Outcome was assessed by the modified Rankin Scale (mRS) at 12 mo, and an unfavorable outcome was defined as an mRS of 4 or 5 or death. Long-term prognostic models were developed using multivariate logistic regression and decision tree algorithms. An additional independent testing dataset was collected for external validation. Overall accuracy, sensitivity, specificity, and area under receiver operating characteristic curves (AUC) were used to assess model performance.

Results: Of the 266 patients, 139 (52.3%) had an unfavorable outcome. Older age, absence of pupillary reactivity, lower Glasgow coma score (GCS), and higher modified Fisher grade were independent predictors of unfavorable outcome. Modified Fisher grade, pupillary reactivity, GCS, and age were used in the decision tree model, which achieved an overall accuracy of 0.833, sensitivity of 0.821, specificity of 0.846, and AUC of 0.88 in the internal test. There was similar predictive performance between the logistic regression and decision tree models. Both models achieved a high overall accuracy of 0.895 in the external test.

Conclusion: Decision tree model is a simple tool for predicting long-term outcomes after poor-grade aSAH and may be considered for treatment decision-making.
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http://dx.doi.org/10.1093/neuros/nyaa052DOI Listing
September 2020