Publications by authors named "Jinhua Ou"

4 Publications

  • Page 1 of 1

1,2-Dibutoxyethane-Promoted Oxidative Cleavage of Olefins into Carboxylic Acids Using O Under Clean Conditions.

J Org Chem 2021 Oct 11. Epub 2021 Oct 11.

Department of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425100, China.

Herein, we report the first example of an effective and green approach for the oxidative cleavage of olefins to carboxylic acids using a 1,2-dibutoxyethane/O system under clean conditions. This novel oxidation system also has excellent functional-group tolerance and is applicable for large-scale synthesis. The target products were prepared in good to excellent yields by a one-pot sequential transformation without an external initiator, catalyst, and additive.
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http://dx.doi.org/10.1021/acs.joc.1c01701DOI Listing
October 2021

The transcriptional characteristics of mast cells derived from skin tissue in type 2 diabetes patients at the single-cell level.

Acta Histochem 2021 Sep 22;123(7):151789. Epub 2021 Sep 22.

Wound Treatment Department, TCM-Integrated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China. Electronic address:

Objectives: The mechanisms underlying the role of mast cells in wound healing have not been thoroughly studied, and even fewer data are available on studies related to mast cells in the skin of patients with type 2 diabetes mellitus (T2DM). Therefore, this study aims to explore the transcriptional characteristics of mast cells at the single-cell level in patients with T2DM and provide experimental data for studying mast cell behaviors under abnormal glucose metabolism.

Methods: Two patients with T2DM and one trauma patient without diabetes were enrolled. Samples were derived from skin tissue resected at the time of surgery and were isolated by single cell capture technology on BD platform to prepare single cell cDNA library. Seurat was used to process raw reads and analyze data downstream of single-cell RNA sequencing, including removal of low-quality cells, identification of cell clusters at the single-cell level, and screening for differential genes with fold change > 1.5 and p < 0.05 by two-sided t-test. We performed single-cell RNA sequencing on skin tissues of T2DM patients and non-diabetics and identified the cell cluster of skin, single-cell subsets, and transcriptional characteristics of mast cells at a single-cell level. Meanwhile, gene set enrichment(GSEA) analysis was performed on the differentially expressed genes.

Results: A total of 8888 cells were obtained from skin tissue. Clustering analysis revealed eight-cell clusters, identified as smooth muscle cells, dendritic cells, mast cells, and T cells, respectively. Cluster 6 was identified as mast cells with the marker genes TPSAB1, CPA3, TPSB2, MS4A2,KIT, etc., which accounting for 2.7% of the total cell number.Compared with the control group, the genes highly expressed in MCs from T2DM patients, include ADH1C, PAXIP1, HAS1, ARG1, etc., and the low expression genes include PHACTR2, GGA1, RASSF2, etc. GSEA analysis suggested that the signal pathways of MCS in T2DM patients included VEGF signaling pathway, Fc gamma R-mediated phagocytosis, the B cell receptor signaling pathway, natural killer cell-mediated cytotoxicity.

Conclusions: The characteristic genes of MCs in the skin tissues of T2DM patients were described at the single-cell level. These genes and enriched signaling pathways provide a theoretical basis and data support for further researches on dermatopathy in patients with diabetes mellitus.
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http://dx.doi.org/10.1016/j.acthis.2021.151789DOI Listing
September 2021

Treatment of Zn in wastewater by sinusoidal alternating current coagulation: response surface methodology and removal mechanism.

Water Sci Technol 2020 Nov;82(9):1950-1960

College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China E-mail:

A novel sinusoidal alternating current coagulation (SACC) technique was used to remove the Zn from wastewater in the present study. The response surface methodology was used to analyze the effect of current density, time, initial pH and initial Zn concentration in order to obtain the optimum removal efficiency and to lower energy consumption. The results show that SACC with a current density of 0.31 A·m applied to treat wastewater containing 120 mg·dm Zn at pH = 9 for 21.3 min can achieve a removal efficiency of Zn of 98.80%, and the energy consumption is 1.147 kWh·m. The main component of flocs produced in SACC process is FeOOH·4HO (HFO). Large specific surface area and good adsorption performance of HFO are demonstrated. There is strong interaction between Zn and HFO. Zn is adsorbed and trapped by HFO and then co-precipitated. Freundlich adsorption isotherm model and pseudo-second order kinetics model explained the Zn adsorption behavior well. The Zn adsorption on HFO is an endothermic and spontaneous process.
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http://dx.doi.org/10.2166/wst.2020.466DOI Listing
November 2020

Surface-Supported Metal-Organic Framework Thin-Film-Derived Transparent [email protected] Carbon Film as an Efficient Counter Electrode for Bifacial Dye-Sensitized Solar Cells.

ACS Appl Mater Interfaces 2019 Apr 12;11(16):14862-14870. Epub 2019 Apr 12.

State Key Laboratory of Fine Chemicals, Institute of Artificial Photosynthesis , Dalian University of Technology , 116024 Dalian , China.

An effective design for counter electrode (CE) catalytic materials with superior catalytic activity, excellent stability, low cost, and a facile fabrication process is urgently needed for industrialization of dye-sensitized solar cells (DSSCs). Herein, we report a facile in situ method to fabricate transparent CoS anchored on an N-doped carbon film electrode through sulfurization of a cobalt-metalloporphyrin metal-organic framework thin film on fluorine-doped tin oxide glass. The transparent film as counter electrode in bifacial DSSCs exhibited higher power conversion efficiency (9.11% and 6.64%), respectively, from front and rear irradiation than that of Pt (8.04% and 5.87%). The uniformly dispersed CoS nanoparticles on an N-doped carbon film provide a large catalytic active area and facilitate the electron transfer, which leads to the excellent catalytic ability of the [email protected] carbon film. In addition, the in situ preparation of the uniform film with a nanosheet structure offers high electrical conductivity and unobstructed access for the diffusion of triiodide to available electroactive sites, resulting in excellent device performance with superior long-term stability over 1000 h under natural conditions.
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http://dx.doi.org/10.1021/acsami.8b21626DOI Listing
April 2019
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