Publications by authors named "Jinhee Park"

120 Publications

Comparative Evaluation of the SMARTCHEK Salmonella species Detection Kit for GENECHECKER UF-300 Real-Time PCR System with Rapi:chipTM and Rapi:DirectTM Lysis Buffer for Select Foods: AOAC Performance Tested MethodsSM 032101.

J AOAC Int 2021 Jul 13. Epub 2021 Jul 13.

Diagnostic Technology Development, Team of Genesystem Co., Ltd.

Background: The candidate method, SMARTCHEK Salmonella spp. Detection Kit assay1, is designed for rapid detection of Salmonella spp. in select food matrixes. For use in conjunction with the Genesystems Rapi: chipTM and GENECHECKERUF-300 real-time PCR system.

Objective: The independent laboratory evaluation consisted of an inclusivity and exclusivity study, product consistency study, robustness study, stability study, and a matrix study. The paired matrix study tested romaine lettuce, peanut butter, liquid whole egg, chicken carcass rinsate and raw chicken. The robustness study evaluated the method by changing three method parameters. The accelerated stability and product consistency study was conducted at 8, 16, 24, 32, and 40 days.

Methods: The candidate method was compared to the U.S. Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook 4.10 Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg, and Siluriformes (Fish) Products and Carcass and Environmental Sponges4 and to the Food and Drug Administration Bacteriological Analytical Manual Chapter 5: Salmonella5 following a paired study design.

Results: All 108 Salmonella isolates were detected in all three broths tested for inclusivity, while all 35 non-Salmonella isolates were correctly excluded. In the matrix study, the candidate method showed no significant differences between confirmed candidate methods results and reference method results for all food matrixes evaluated.

Conclusions: The candidate method is an effective method for the qualitative detection of Salmonella spp. in select food matrixes.

Highlight: The SMARTCHEK Salmonella spp. Detection Kit assay allows for fast, reliable detection of Salmonella spp. with results obtained after 24 h of incubation and a PCR run time of only 35 min.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jaoacint/qsab090DOI Listing
July 2021

Combined exercise and nutrition intervention for spinal sarcopenia: A pilot study protocol.

Medicine (Baltimore) 2021 Jun;100(24):e26421

Department of Rehabilitation Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam-si, Gyeonggi-do, Republic of Korea.

Introduction: Spinal sarcopenia is a multifactorial disorder associated with the atrophy of and fatty changes to the paraspinal muscles. We previously developed the concept of spinal sarcopenia in community-dwelling older adults and investigated the association between conventional sarcopenic indices and spinal sarcopenia. However, interventional studies of spinal sarcopenia are lacking. This pilot study will aim to evaluate the effectiveness of a combined exercise and nutrition intervention for treating spinal sarcopenia.

Methods And Analysis: This open-label single-arm prospective study will include 35 community-dwelling older women who were diagnosed with spinal sarcopenia in our previous cohort study. The 12-week combined intervention will consist of back extensor strengthening exercise and nutritional supplementation. The primary outcome of this study will be isometric back extensor strength after the 12-week intervention. All functional and radiographic outcomes will be measured at 0, 12, and 24 weeks post-intervention. The data will be analyzed using the intention-to-treat principle.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000026421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213246PMC
June 2021

Cost Effectiveness of Ribociclib in Combination with Fulvestrant for the Treatment of Postmenopausal Women with HR+/HER2- Advanced Breast Cancer Who Have Received No or Only One Prior Line of Endocrine Therapy: A Canadian Healthcare Perspective.

Pharmacoeconomics 2021 Jun 9. Epub 2021 Jun 9.

Policy Analysis Inc. (PAI), 822 Boylston Street, Suite 206, Chestnut Hill, MA, 02467, USA.

Background: The MONALEESA-3 trial demonstrated the efficacy and safety of ribociclib plus fulvestrant versus placebo plus fulvestrant for patients with hormone receptor-positive and human epidermal growth factor receptor 2-negative (HR+/HER2-) advanced breast cancer (ABC). This analysis evaluated the cost effectiveness of ribociclib plus fulvestrant versus fulvestrant in patients with HR+/HER2- ABC from a Canadian healthcare payer perspective.

Methods: The incremental cost-effectiveness ratio (ICER), expressed as incremental costs per quality-adjusted life-year (QALY) gained for ribociclib plus fulvestrant versus fulvestrant, was estimated using a semi-Markov cohort model developed in Microsoft Excel, with states for progression-free, post-progression, and dead. A 15-year time horizon was used. Survival distributions for progression-free survival (PFS), post-progression survival (PPS), and time to discontinuation (TTD) were based on parametric survival distributions fit to data from MONALEESA-3. Health-state utilities were estimated using EQ-5D index values collected in MONALEESA-3. Direct costs of ABC treatment (medication and administration costs, follow-up and monitoring, adverse events, subsequent treatments) were based on Canadian-specific values from published sources. Costs (2019 CAN$) and QALYs were discounted at 1.5% annually.

Results: In the base case, ribociclib plus fulvestrant was estimated to result in gains of 1.19 life-years and 0.96 QALYs versus fulvestrant, at an incremental cost of $151,371. The ICER of ribociclib plus fulvestrant versus fulvestrant was $157,343 per QALY gained based on the mean of probabilistic analyses. Results were sensitive to parametric distributions used for projecting long-term TTD, PFS, and PPS.

Conclusions: For patients with HR+/HER2- ABC, ribociclib plus fulvestrant is projected to result in substantial gains in QALYs compared with fulvestrant. At its current list price, ribociclib used in combination with fulvestrant is likely to be cost effective in these patients at a threshold ICER of $157,343. These results may be useful in deliberations regarding reimbursement and access to this treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40273-021-01027-4DOI Listing
June 2021

Novel lateral whole-body dual-energy X-ray absorptiometry of lumbar paraspinal muscle mass: results from the SarcoSpine study.

J Cachexia Sarcopenia Muscle 2021 May 28. Epub 2021 May 28.

DInstitute of Aging, Seoul National University, Seoul, South Korea.

Background: Here, we aimed to propose novel lateral whole-body dual-energy X-ray absorptiometry (lateral DXA) as a simple tool for measuring spinal muscle mass and investigate the feasibility of lateral DXA to measure lumbar paraspinal muscle (LPM) mass compared with lumbosacral spine three-dimensional magnetic resonance imaging (3D MRI).

Methods: Twenty consecutive participants were enrolled from a prospective observational cohort (SarcoSpine study). Lateral DXA was scanned with each participant in the lateral decubitus position. The region of interest was defined to analyse the LPM mass. LPM total volume, LPM cross-sectional area at the L3 mid-vertebra and L4/5 mid-disc levels and each signal intensity were measured by 3D MRI. Isokinetic and isometric back extensor muscle strengths as well as back extensor endurance were examined. The correlation between lateral DXA-based mass (weight) and 3D MRI-based LPM volume was analysed.

Results: The mean age of the 20 participants (15 women, 5 men) was 72.2 ± 4.9 years. LPM mass by lateral DXA was positively correlated with LPM volume by 3D MRI (β = 0.333, r = 0.692, p < 0.001) and negatively correlated with signal intensity of the total LPM (β = -0.263, r = -0.530, p = 0.016). LPM mass was also correlated with appendicular limb muscle mass, handgrip strength and gait speed as well as back extensor endurance (r = 0.620, p = 0.004).

Conclusions: Our data suggest that LPM mass assessed by lateral DXA was positively correlated with LPM volume by 3D MRI in older adults. Lateral DXA may be a potential substitute for the cross-sectional area measurement of LPM mass. Further studies are required to validate this lateral DXA technique.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcsm.12721DOI Listing
May 2021

The use of eggshell membrane for the treatment of dye-containing wastewater: Batch, kinetics and reusability studies.

Chemosphere 2021 Oct 11;281:130777. Epub 2021 May 11.

Department of Environmental Science and Engineering, Ewha Womans University, 11-1 Daehyun-Dong, Seodaemun-Gu, Seoul, 120-750, South Korea. Electronic address:

The worldwide consumption of eggs is very high, leading to about 250,000 tons of eggshell membrane (ESM) waste annually. The present research thus investigated the potential use of ESM as an inexpensive and abundant adsorbent for Reactive Red 120 (RR120) in aqueous solutions, a widespread hydrophilic azo dye used in the textile industry. The chemical structure and morphology of ESM were characterized using various spectroscopic methods, including scanning electron microscopy, Fourier transform infrared spectroscopy, and elemental analysis. It was found that natural ESM has a porous structure and surface functional groups that are suitable for the adsorption of the target molecules. The impact of the operating conditions, including the variation in the pH and temperature, on the RR120 sorption capacity and mechanisms of ESM was also analyzed. The maximum monolayer adsorption ability of ESM for RR120 was found to be 191.5 mg/g at 318 K, and the sorption process was spontaneous and endothermic. The adsorption of RR120 onto ESM was significantly influenced by the solution pH and the use of NaOH as eluent, indicating that the driving force for this adsorption was electrostatic attraction. Subsequent desorption experiments using 0.1 M NaOH resulted in satisfactory recovery efficiency. Kinetic, isothermic, and thermodynamic analysis was also conducted to support the experimental findings. The experimental results for the adsorption kinetics of ESM were fitted by a pseudo-second-order model. In conclusion, ESM has the potential to be utilized as an eco-friendly and cost-effective adsorbent for the removal of RR120 from aqueous solutions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.130777DOI Listing
October 2021

Cost Effectiveness of Ribociclib Plus a Nonsteroidal Aromatase Inhibitor in Pre-/Perimenopausal, HR+ and HER2- Advanced Breast Cancer: A Canadian Healthcare Perspective.

Pharmacoeconomics 2021 Jul 18;39(7):853-867. Epub 2021 May 18.

Policy Analysis Inc. (PAI), 822 Boylston Street, Suite 206, Chestnut Hill, MA, 02467, USA.

Background And Objectives: The MONALEESA-7 trial demonstrated the efficacy and safety of ribociclib plus a nonsteroidal aromatase inhibitor (NSAI) [with goserelin] for pre-/perimenopausal women with hormone receptor-positive and human epidermal growth factor receptor 2-negative advanced breast cancer. This analysis evaluated the cost effectiveness of ribociclib plus NSAI vs NSAI monotherapy and tamoxifen monotherapy from the perspective of the Canadian healthcare system.

Methods: The incremental cost-effectiveness ratio expressed as incremental costs per quality-adjusted life-year (QALY) gained for ribociclib plus an NSAI vs an NSAI and vs tamoxifen was estimated using a semi-Markov cohort model developed in Microsoft Excel with a 15-year time horizon and states for progression-free survival, post-progression survival, and dead. Survival distributions for progression-free survival, post-progression survival, and time to discontinuation as well as health-state utilities were estimated using data from MONALEESA-7. Direct costs of advanced breast cancer treatment were based on Canadian-specific values from published sources. Costs ($CAN 2019) and QALYs were discounted at 1.5% annually.

Results: Ribociclib plus an NSAI was estimated to yield gains of 1.42 life-years and 1.17 QALYs vs an NSAI, and 2.61 life-years and 2.12 QALYs vs tamoxifen, at incremental costs of $209,701 and $220,836, respectively. In probabilistic analyses, the incremental cost-effectiveness ratio for ribociclib plus an NSAI was estimated to be $178,872 per QALY gained vs an NSAI and $104,400 per QALY gained vs tamoxifen. Results of deterministic analyses were similar (incremental cost-effectiveness ratios of $177,245 and $103,316 vs NSAI and tamoxifen, respectively). Results were sensitive to parametric distributions used for projecting progression-free survival and the time horizon.

Conclusions: At its current list price, ribociclib used in combination with NSAI is likely to be co-effective relative to an NSAI alone or tamoxifen alone if the willingness-to-pay threshold is less than approximately $178,000 per QALY. These results have informed deliberations regarding reimbursement and access to this treatment in Canada and may be useful for decision makers in other settings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40273-021-01028-3DOI Listing
July 2021

Effectiveness of Alpelisib + Fulvestrant Compared with Real-World Standard Treatment Among Patients with HR+, HER2-, PIK3CA-Mutated Breast Cancer.

Oncologist 2021 07 13;26(7):e1133-e1142. Epub 2021 May 13.

University of California San Francisco Helen Diller Family Comprehensive Cancer Center, San Francisco, California, USA.

Background: The BYLieve trial (NCT03056755) confirmed efficacy and safety of alpelisib with fulvestrant for hormone receptor-positive (HR+), human epidermal growth factor receptor-2-negative (HER2-), PIK3CA-mutated advanced breast cancer (ABC), after cyclin-dependent kinase 4/6 inhibitor (CDK4/6i) with an aromatase inhibitor (AI) as immediate prior therapy. Further analyses were performed to compare efficacy from BYLieve with effectiveness of standard treatment in the real-world setting.

Materials And Methods: Patients who progressed on a CDK4/6i plus AI and were treated with alpelisib with fulvestrant in BYLieve were matched with a real-world patient cohort who received standard-of-care from a deidentified clinico-genomics database (CGDB). Primary and secondary endpoints were to compare progression-free survival (PFS), estimated by the Kaplan-Meier method, and the proportion of patients remaining progression-free at 6 months, respectively, between the two cohorts.

Results: A total of 855 patients with PIK3CA-mutant disease who had prior CDK4/6i plus hormone therapy were selected from the CGDB; further matching to 120 patients from BYLieve selected 95 patients without exposure to HER2-targeting agents, clinical study drug, or alpelisib. In unadjusted and postmatching results, primary and secondary endpoints favored treatment with alpelisib with fulvestrant in BYLieve more than standard treatments in the real-world cohort. Postadjustment, median PFS for patients treated with alpelisib in BYLieve was 7.3 versus 3.7 months in the real-world cohort, and 6-month PFS was 54.6% versus 40.1%, respectively.

Conclusion: Matched/weighted analysis comparing BYLieve with the real-world setting further supports the clinical benefit of alpelisib with fulvestrant for treatment of HR+, HER2-, PIK3CA-mutant ABC after CDK4/6i treatment.

Implications For Practice: Approximately 40% of patients with hormone receptor-positive (HR+), human epidermal growth factor receptor-2-negative (HER2-) advanced breast cancer (ABC) have PIK3CA-mutated tumors, which have been associated with endocrine therapy resistance. Alpelisib, an α-selective phosphatidylinositol-3-kinase inhibitor, demonstrated significantly improved progression-free survival in SOLAR-1 and demonstrated clinical efficacy in BYLieve when combined with fulvestrant. Data are limited in comparing the efficacy of alpelisib combined with fulvestrant with effectiveness of standard therapy after CDK4/6i treatment. Using real-world data, this is the first analysis comparing alpelisib combined with fulvestrant with standard treatments for HR+, HER2-, PIK3CA-mutant ABC in the post-CDK4/6i setting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/onco.13804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265362PMC
July 2021

Patient-Reported Outcomes in Patients With -Mutated Hormone Receptor-Positive, Human Epidermal Growth Factor Receptor 2-Negative Advanced Breast Cancer From SOLAR-1.

J Clin Oncol 2021 Jun 29;39(18):2005-2015. Epub 2021 Mar 29.

Department of Medical Oncology, Institut Gustave Roussy, Villejuif, France.

Purpose: In the phase III SOLAR-1 trial (NCT02437318), the PI3Kα-selective inhibitor and degrader alpelisib significantly improved median progression-free survival when added to fulvestrant in patients with phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha ()-mutated, hormone receptor-positive, human epidermal growth factor receptor 2-negative advanced breast cancer. We assessed health-related quality of life using patient-reported outcome measures in these patients.

Materials And Methods: In the -mutant cohort, 341 patients were randomly assigned 1:1 to receive alpelisib 300 mg daily or placebo plus fulvestrant 500 mg on days 1 and 15 of cycle 1 and on day 1 of subsequent 28-day cycles. Patient-reported outcomes were evaluated with the European Organisation for Research and Treatment of Cancer QoL of Cancer Patients and Brief Pain Inventory-Short Form questionnaires. Changes from baseline and time to 10% deterioration were analyzed using repeated measurement models and Cox models, respectively.

Results: Global Health Status/QoL and functional status were maintained from baseline (mean changes < 10 points) in the alpelisib (overall change from baseline [95% CI], -3.50 [-8.02 to 1.02]) and placebo arms (overall change from baseline [95% CI], 0.27 [-4.48 to 5.02]). Overall treatment effect in Global Health Status/QoL was not significantly different between arms (-3.77; 95% CI, -8.35 to 0.80; = .101). Time to 10% deterioration for Global Health Status/QoL was similar between arms (hazard ratio, 1.03; 95% CI, 0.72 to 1.48). Compared with placebo, deterioration in social functioning and in diarrhea, appetite loss, nausea or vomiting, and fatigue symptom subscales occurred with alpelisib. Numerical improvement in Worst Pain was observed with alpelisib versus placebo (42% 32%, week 24; = .090).

Conclusion: In SOLAR-1, there was no statistical difference in deterioration of Global Health Status/QoL between arms, whereas symptom subscales favored placebo for diarrhea, appetite loss, nausea or vomiting, and fatigue, known side effects of alpelisib. Treatment decisions must consider efficacy and tolerability; taken with clinical efficacy, these results support the benefit-risk profile of alpelisib in patients with hormone receptor-positive, human epidermal growth factor receptor 2-negative -mutated advanced breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1200/JCO.20.01139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210974PMC
June 2021

The Impact of Feeding on the Parent and Family Scales (Feeding Impact Scales): Development and Psychometric Testing.

J Nurs Meas 2020 Nov 16. Epub 2020 Nov 16.

Connell School of Nursing, Boston College, Newton, MA.

Background And Purpose: Families of children with feeding disorder face significant challenges in supporting their child's feeding, growth, and development. The Feeding Impact Scales were developed to assess how child feeding impacts parent and family.

Methods: Items were adapted from an existing scale. Parents of children with feeding difficulty completed the online survey. Item response theory (IRT) analyses were used to evaluate and reduce items. Internal consistency reliability, convergent validity, and readability were tested.

Results: IRT analyses ( = 317) identified 12 items for the Parent Impact and 13 items for the Family Impact. Internal reliability for the scales were acceptable. Convergent validity was supported.

Conclusions: The Feeding Impact Scales have evidence of reliability and validity. They can be utilized in practice and research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1891/JNM-D-20-00008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132290PMC
November 2020

Bottle-Feeding Challenges in Preterm-Born Infants in the First 7 Months of Life.

Glob Pediatr Health 2020 4;7:2333794X20952688. Epub 2020 Sep 4.

William F. Connell School of Nursing, Boston College, Chestnut Hill, MA, USA.

Preterm infants frequently experience oral feeding challenges while in the neonatal intensive care unit, with research focusing on infant feeding during this hospital stay. There is little data on symptoms of problematic feeding in preterm-born infants in the months after discharge. The purpose of this study was to describe symptoms of problematic bottle-feeding in the first 7 months of life in infants born preterm, compared to full-term infants. Parents of infants less than 7 months old completed an online survey that included the Neonatal Eating Assessment Tool-Bottle-feeding and questions about the infant's medical and feeding history. General linear models were used to evaluate differences in NeoEAT-Bottle-feeding total score and subscale scores by preterm category, considering other significant factors. Very preterm infants had more symptoms of problematic bottle-feeding than other infants. Current age, presence of gastroesophageal reflux, and anomalies of the face/mouth were associated with problematic bottle-feeding.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/2333794X20952688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479869PMC
September 2020

Agreement of Postoperative Pain Assessment by Parents and Clinicians in Children Undergoing Orthopedic Surgery.

J Trauma Nurs 2020 Sep/Oct;27(5):302-309

Department of Orthopedic Surgery, Asan Medical Center Children's Hospital, Seoul, South Korea (Dr Kang); and College of Nursing, Seoul National University, Seoul, South Korea (Ms Park and Dr Kim).

Background: Postoperative pain assessment in children is crucial for proper nursing care. However, research on the agreement of these assessments with a patient's self-reported pain level is lacking. The aim was to investigate the agreement between patients' subjective postoperative pain and corresponding observers' pain assessments.

Methods: In children who underwent orthopedic limb surgery, we investigated the agreement between their reported pain levels and observers' pain assessments using the Numeric Pain Rating Scale (NPRS) and the Faces Pain Scale-Revised (FPS-R) on postoperative day 1. The parents (specifically the mother) and clinicians (a doctor and a nurse in the field of pediatric orthopedics) participated as observers. Reliabilities using intraclass correlation coefficients (ICCs) and correlations using Spearman's coefficients (rs) were calculated.

Results: The patients' pain intensities (2.1± 2.1 [NPRS] and 2.0 ± 1.9 [FPS-R]) were overestimated by parents (2.5 ± 2.0 [NPRS], p = .022), but underestimated by clinicians (1.5 ± 1.2 [NPRS], p < .001, and 1.5 ± 1.2 [FPS-R], p = .006). The overall reliabilities between parents and patients were good (ICCs > 0.75 for both NPRS and FPS-R), and the correlations between them were even strong in some circumstances, such as when the patient was a girl, the diagnostic type was a disease, or the lower extremity was operated (rss > 0.7). However, the agreements between clinicians and patients were only moderate.

Conclusions: The subjective pediatric postoperative pain intensities had different agreements according to the observers. The parents' assessments should be considered for the accurate assessments, but their tendency to overestimate their children's pain should also be taken into account.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/JTN.0000000000000533DOI Listing
September 2020

Initial angular deformity and its recovery of the proximal tibial metaphyseal fracture according to the cause: trampoline-related injury other injuries.

J Child Orthop 2020 Aug;14(4):304-311

Department of Orthopedic Surgery, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Purpose: This study investigated the initial angular deformity of proximal tibial metaphyseal fracture in children and its recovery during follow-up according to the cause of injury.

Methods: Prospective data about the patients with a proximal tibial metaphyseal fracture at the age less than six years and available follow-up data at two years post-trauma were retrospectively reviewed. They were grouped into trampoline-related injuries (TRI) and non-TRI groups based on the cause of injury. Proximal tibial valgus and recurvatum angles were measured to assess angular deformity on the coronal and sagittal planes, respectively.

Results: A total of 47 patients (33 TRI and 14 non-TRI) were included. Initially, the valgus angles were -1.5° in TRI and 1.6° in non-TRI groups (p < 0.001) and the recurvatum angles were 7.8° in TRI and 4.1° in non-TRI groups (p = 0.048). After two-year follow-up, the valgus angles were 0.2° in TRI and 0.9° in non-TRI groups (p = 0.070), and the recurvatum angles were 6.5° in TRI and 2.3° in non-TRI groups (p = 0.001).

Conclusion: For children with a proximal tibial metaphyseal fracture, the initial coronal deformity was different according to the injury cause (varus in TRI whereas valgus in non-TRI). Although there was a near complete recovery after approximately two years of follow-up in the coronal deformities, the sagittal deformity (genu recurvatum) seems to recover incompletely or tardily, especially for those caused by TRI.

Level Of Evidence: III.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1302/1863-2548.14.200072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453164PMC
August 2020

Reduction of bioaccessibility of As in soil through in situ formation of amorphous Fe oxides and its long-term stability.

Sci Total Environ 2020 Nov 17;745:140989. Epub 2020 Jul 17.

Department of Civil & Environmental Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea. Electronic address:

The bioaccessibility of As in soil, rather than its total concentration, is closely related to its potential risk. In this study, the in situ formation of amorphous Fe oxides was applied to As-contaminated soil to induce As-Fe coprecipitates that can withstand the gastric digestion condition of human beings. To promote the formation of Fe oxides, 2% ferric nitrate (w/w) and 30% water (v/w) were introduced, and the pH was adjusted to ~7. The chemical extractability of As in soil was determined using the solubility/bioavailability research consortium method and five-step sequential extraction. In situ formation of Fe oxides resulted in a remarkable increase in the As associated with amorphous Fe oxides, decreasing most of the exchangeable As (i.e., the sum of SO and PO extractable As), and thereby reducing the bioaccessibility of As. The types of association between As and Fe oxides were investigated using X-ray absorption spectroscopy analysis. Linear combination fit (LCF) analysis demonstrated that As bound to amorphous Fe oxides could exist as coprecipitates with ferrihydrite and schwertmannite after stabilization. The bioaccessibility of the coprecipitated As in soil further decreased as amorphous Fe oxides transformed to crystalline form with time, which was supported by the LCF results showing an increase of goethite in aged soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.140989DOI Listing
November 2020

Synthesis and Photocatalytic Properties of Titanium-Porphyrinic Aerogels.

Angew Chem Int Ed Engl 2020 Nov 17;59(48):21591-21596. Epub 2020 Sep 17.

Department of Emerging Materials Science, Daegu-Gyeongbuk Institute of Science & Technology, Daegu, 42988, Republic of Korea.

We present novel titanium-porphyrinic gels (TPGs) and titanium-porphyrinic aerogels (TPAs), in which porphyrinic ligand tetrakis(4-carboxyphenyl)porphyrin is coordinated to Ti-oxo clusters. These hierarchically porous TPAs, with micro-, meso-, and macropores and reactant-concentration-dependent Brunauer-Emmett-Teller surface areas of 407-738 m  g , are prepared by CO critical point drying of TPGs. Although the Ti → Ti photoreduction of TPAs is less efficient than that of crystalline microporous Ti-porphyrinic framework DGIST-1, prompt diffusion of O and spin-trapping agents into the TPA pores causes the rapid generation of reactive oxygen species (ROS), as observed by EPR spectroscopy. When used as an ROS scavenger, large 1,3-diphenylisobenzofuran is degraded by the best-performing TPA 10 times faster than by DGIST-1, suggesting that the accessibility of molecules (reactants) to pores (reactive centers) strongly influences photocatalytic activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202007193DOI Listing
November 2020

A practical application of generative adversarial networks for RNA-seq analysis to predict the molecular progress of Alzheimer's disease.

PLoS Comput Biol 2020 07 24;16(7):e1008099. Epub 2020 Jul 24.

Dementia Research Group, Korea Brain Research Institute (KBRI), Daegu, Korea.

Next-generation sequencing (NGS) technology has become a powerful tool for dissecting the molecular and pathological signatures of a variety of human diseases. However, the limited availability of biological samples from different disease stages is a major hurdle in studying disease progressions and identifying early pathological changes. Deep learning techniques have recently begun to be applied to analyze NGS data and thereby predict the progression of biological processes. In this study, we applied a deep learning technique called generative adversarial networks (GANs) to predict the molecular progress of Alzheimer's disease (AD). We successfully applied GANs to analyze RNA-seq data from a 5xFAD mouse model of AD, which recapitulates major AD features of massive amyloid-β (Aβ) accumulation in the brain. We examined how the generator is featured to have specific-sample generation and biological gene association. Based on the above observations, we suggested virtual disease progress by latent space interpolation to yield the transition curves of various genes with pathological changes from normal to AD state. By performing pathway analysis based on the transition curve patterns, we identified several pathological processes with progressive changes, such as inflammatory systems and synapse functions, which have previously been demonstrated to be involved in the pathogenesis of AD. Interestingly, our analysis indicates that alteration of cholesterol biosynthesis begins at a very early stage of AD, suggesting that it is the first effect to mediate the cholesterol metabolism of AD downstream of Aβ accumulation. Here, we suggest that GANs are a useful tool to study disease progression, leading to the identification of early pathological signatures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.pcbi.1008099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406107PMC
July 2020

Effect of neutralizing agents on the type of As co-precipitates formed by in situ Fe oxides synthesis and its impact on the bioaccessibility of As in soil.

Sci Total Environ 2020 Nov 3;743:140686. Epub 2020 Jul 3.

Department of Civil & Environmental Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.

The bioaccessibility of heavy metals in soil is closely related to their potential risk. Therefore, developing techniques for reducing it needs considerable attention. In this study, we aimed to co-precipitate soil As(V) through an in situ formation of Fe oxides, thereby reducing its bioaccessibility. Soil As(V) was co-precipitated by introducing 2% Fe-nitrate (w/w) and 30% water (v/w) into soil at pH ~7. Two different neutralizing agents (NaOH and CaO) were used to induce the precipitation of Fe oxides, and their effects on the speciation of As were investigated. In all the stabilized soils, the exchangeable As fraction decreased, and the fraction of As bound to amorphous Fe oxides increased by a factor of more than 1.4. In contrast, a marked decrease in bioaccessibility of As was achieved using NaOH (40% to 7%). X-ray absorption spectroscopy analysis demonstrated that highly bioaccessible forms of calcium iron arsenate (yukonite and arseniosiderite) could be generated in CaO-stabilized soil. Our study found that neutralizing agents may play an important role in stabilizing As(V) and lowering its bioaccessibility through determining the type of formed Fe oxides in soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.140686DOI Listing
November 2020

Microscopic and Mesoscopic Dual Postsynthetic Modifications of Metal-Organic Frameworks.

Angew Chem Int Ed Engl 2020 Aug 3;59(33):13793-13799. Epub 2020 Jun 3.

Department of Emerging Materials Science, Daegu-Gyeongbuk Institute of Science and Technology (DGIST), 333 Techno Jungang-daero, Dalseong-gun, Daegu, 42988, Korea.

We report the dual postsynthetic modification (PSM) of a metal-organic framework (MOF) involving the microscopic conversion of C-H bonds into C-C bonds and the mesoscopic introduction of hierarchical porosity. MOF crystals underwent single-crystal-to-single-crystal transformations during the electrophilic aromatic substitution of Co (m-DOBDC) (m-DOBDC =4,6-dioxo-1,3-benzenedicarboxylate) with alkyl halides and formaldehyde. The steric hindrance caused by the proximity of the introduced functional groups to the coordination bonds reduced bond stability and facilitated the transformation into hierarchically porous mesostructures by etching with in situ generated protons (hydroniums) and halides. The numerous defect sites in the mesostructural MOFs are potential water-sorption sites. However, since the introduced functional groups are close to the main adsorption sites, even methyl groups are able to considerably decrease water adsorption, whereas hydroxy groups increase adsorption at low vapor pressures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.202000278DOI Listing
August 2020

Directly drawn top-gate semiconducting carbon nanotube thin-film transistors and complementary inverters.

Nanotechnology 2020 Aug 22;31(32):32LT01. Epub 2020 Apr 22.

School of Electrical Engineering, Kookmin University, Seoul 02707, Republic of Korea.

As the emerging demand for electronic devices that are simple, cost effective and capable of rapid fabrication has increased, novel fabrication techniques for designing and manufacturing such devices have attracted remarkable research interest. One method for prototyping these electronic devices is to draw them using a handwriting tool that is commonly available. In this work, we demonstrate a transistor and complementary logic inverter that are directly drawn using a brush and that are based on solution-based materials such as semiconducting carbon nanotubes (CNTs), silver ink and paste, and cross-linked poly(4-vinylphenol) (cPVP). The directly drawn CNT thin-film transistor (TFT) has p-type behavior due to the adsorption of oxygen and moisture, a high current on/off ratio (approximately 10), and a low operating voltage. By employing a solution-based chemical doping treatment with an amine-rich polymer, polyethyleneimine (PEI), that has strong electron-donating ability, the drawn p-type CNT-TFT is successfully converted to an n-type CNT-TFT. Therefore, we fabricate a drawn complementary logic inverter consisting of the p-type CNT-TFT and PEI-treated n-type CNT-TFT and evaluate its electrical performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/ab8c06DOI Listing
August 2020

Sleep Promotion for Preterm Infants in the NICU.

Authors:
Jinhee Park

Nurs Womens Health 2020 Feb 19;24(1):24-35. Epub 2020 Feb 19.

Sleep is a crucial human physiologic need. Preterm infants in the NICU are exposed to noxious stimuli that often disrupt and shorten their sleep periods. Sleep disruption may have a negative effect on clinical outcomes, growth, and development and may also delay hospital discharge. Increasing evidence suggests that sleep quality is critical for brain development and synaptic plasticity and is associated with long-term neurodevelopmental outcomes. The purpose of this article is to discuss the importance of sleep in preterm infants, describe tools commonly used to assess infant sleep and identify different sleep-wake states, and identify interventions that promote sleep in preterm infants in the NICU. Nurses play a vital role in implementing appropriate interventions that promote preterm infants' sleep.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nwh.2019.11.004DOI Listing
February 2020

Graphene-assisted spontaneous relaxation towards dislocation-free heteroepitaxy.

Nat Nanotechnol 2020 Apr 10;15(4):272-276. Epub 2020 Feb 10.

Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Although conventional homoepitaxy forms high-quality epitaxial layers, the limited set of material systems for commercially available wafers restricts the range of materials that can be grown homoepitaxially. At the same time, conventional heteroepitaxy of lattice-mismatched systems produces dislocations above a critical strain energy to release the accumulated strain energy as the film thickness increases. The formation of dislocations, which severely degrade electronic/photonic device performances, is fundamentally unavoidable in highly lattice-mismatched epitaxy. Here, we introduce a unique mechanism of relaxing misfit strain in heteroepitaxial films that can enable effective lattice engineering. We have observed that heteroepitaxy on graphene-coated substrates allows for spontaneous relaxation of misfit strain owing to the slippery graphene surface while achieving single-crystalline films by reading the atomic potential from the substrate. This spontaneous relaxation technique could transform the monolithic integration of largely lattice-mismatched systems by covering a wide range of the misfit spectrum to enhance and broaden the functionality of semiconductor devices for advanced electronics and photonics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41565-020-0633-5DOI Listing
April 2020

State Vocational Rehabilitation Services and Employment Outcomes for Asian Americans with Psychiatric Disabilities.

Community Ment Health J 2020 07 10;56(5):854-866. Epub 2020 Jan 10.

Department of Counseling, Educational Psychology and Special Education, Michigan State University, East Lansing, MI, USA.

One major concern of Asian Americans with psychiatric disabilities is underutilization of services, furthermore their service needs and accessibility have been significantly understudied. This study examined the effects of the public vocational rehabilitation (VR) services on employment outcomes for Asian Americans with psychiatric disabilities in the United States. This study investigated which individual characteristics, work disincentives, and VR service types were related to competitive employment outcomes among Asian Americans with psychiatric disabilities and compared the findings to other racial/ethnic groups. Logistic regression analyses were used to analyze a sample of RSA-911 data from fiscal year 2013. The results provided empirical support regarding VR services and employment outcomes for Asian Americans with psychiatric disabilities. Specifically, level of education, work experiences, and receipt of health insurance benefits were significantly related to employment outcomes among the Asian American group. Regarding VR services, job placement assistance, on-the-job supports, maintenance, miscellaneous training, and other services also predicted employment outcomes. Future research needs to address the impact of specific cultural factors on access to VR services, service utilization, and employment outcomes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10597-020-00547-1DOI Listing
July 2020

Sleep-Wake States and Feeding Progression in Preterm Infants.

Nurs Res 2020 Jan/Feb;69(1):22-30

Jinhee Park, PhD, RN, is Assistant Professor, School of Nursing, Boston College, Chestnut Hill, Massachusetts. Susan G. Silva, PhD, is Associate Professor, School of Nursing, Duke University, Durham, North Carolina. Suzanne M. Thoyre, PhD, RN, FANN, is Professor, School of Nursing, University of North Carolina at Chapel Hill. Debra H. Brandon, PhD, RN, CNS, FAAN, is Associate Professor, School of Nursing, Duke University, Durham, North Carolina.

Background: Previous studies demonstrated a short-term relationship between infant sleep-wake states and oral feeding performance, with state being an indication of infants' neurobehavioral readiness for feeding. However, the relationship between sleep-wake states and feeding skills has not been evaluated longitudinally during hospitalization.

Objectives: The purpose of this study was to examine preterm infants' sleep-wake state developmental trajectories and their associations with feeding progression during hospitalization.

Methods: This descriptive and exploratory study was a secondary analysis using data from a longitudinal two-group, randomized controlled trial evaluating the effects of early and late cycled light on health and developmental outcomes among extremely preterm infants who were born ≤28 weeks of gestational age. Sleep-wake states were assessed for two 2-hour interfeeding periods per day (day and night hours), 30 weeks postmenstrual age, and every 3 weeks until discharge. Occurrences of active sleep, quiet sleep, and waking were recorded every 10 seconds. Feeding progression was assessed based on an infant's postmenstrual age at five milestones: first enteral feeding, full enteral feeding, first oral feeding, half oral feeding, and full oral feeding. Trajectory analyses were used to describe developmental changes in sleep-wake states, feeding progression patterns, and associations between feeding progression and sleep-wake trajectories.

Results: Active sleep decreased while waking, and quiet sleep increased during hospitalization. Two distinct feeding groups were identified: typical and delayed feeding progression. In infants with delayed feeding progression, rates of active and quiet sleep development during the day were delayed compared to those with typical feeding progression. We also found that infants with delayed feeding progression were more likely to be awake more often during the night compared to infants with typical feeding progression.

Discussions: Findings suggest that delays in sleep-wake state development may be associated with delays in feeding progression during hospitalization. Infants with delayed feeding skill development may require more environmental protection to further support their sleep development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/NNR.0000000000000395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6919212PMC
April 2020

Stimuli-Responsive Ti-Organic Gels and Aerogels Derived from Ti-Oxo Clusters: Hierarchical Porosity and Photocatalytic Activity.

Inorg Chem 2019 Dec 19;58(23):15936-15941. Epub 2019 Nov 19.

Department of Emerging Materials Science , Daegu Gyeongbuk Institute of Science and Technology (DGIST) , 333 Techno Jungang-daero , Dalseong-gun, Daegu 42988 , South Korea.

Herein, we report titanium-organic gels (TOGs) as new Ti-oxo-based materials that exhibit stimuli-responsive sol-gel transformations and hierarchical porosity upon the removal of solvent molecules. Heating a solution of Ti-oxo clusters and pyromellitic acid as a tetratopic ligand produces TOGs that readily become sols by applying physical stimuli such as shaking or vortexing under ambient conditions. Porous titanium-organic aerogels (TOAs) were obtained by the CO supercritical point drying (CPD) of the TOGs, and their porous structures were characterized by N adsorption and desorption isotherm measurements. These TOAs, based on the Ti-oxo clusters, possess hierarchical micro-, meso-, and macropores. Furthermore, accompanying the prominent photochromic phenomena, reduction of Ti to Ti was observed upon UV irradiation. The TOAs were successfully applied in the adsorption and photocatalytic degradation of several dye molecules. This research introduces a versatile method for preparing stimuli-responsive and porous Ti-oxo-based photocatalysts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.inorgchem.9b02444DOI Listing
December 2019

BMAL1 associates with chromosome ends to control rhythms in TERRA and telomeric heterochromatin.

PLoS One 2019 21;14(10):e0223803. Epub 2019 Oct 21.

Department of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, United States of America.

The circadian clock and aging are intertwined. Disruption to the normal diurnal rhythm accelerates aging and corresponds with telomere shortening. Telomere attrition also correlates with increase cellular senescence and incidence of chronic disease. In this report, we examined diurnal association of White Collar 2 (WC-2) in Neurospora and BMAL1 in zebrafish and mice and found that these circadian transcription factors associate with telomere DNA in a rhythmic fashion. We also identified a circadian rhythm in Telomeric Repeat-containing RNA (TERRA), a lncRNA transcribed from the telomere. The diurnal rhythm in TERRA was lost in the liver of Bmal1-/- mice indicating it is a circadian regulated transcript. There was also a BMAL1-dependent rhythm in H3K9me3 at the telomere in zebrafish brain and mouse liver, and this rhythm was lost with increasing age. Taken together, these results provide evidence that BMAL1 plays a direct role in telomere homeostasis by regulating rhythms in TERRA and heterochromatin. Loss of these rhythms may contribute to telomere erosion during aging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223803PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6802832PMC
March 2020

NANOG helps cancer cells escape NK cell attack by downregulating ICAM1 during tumorigenesis.

J Exp Clin Cancer Res 2019 Oct 16;38(1):416. Epub 2019 Oct 16.

Division of Gene Therapy Science, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.

Background: At the beginning of tumorigenesis, newly born cancer cells must successfully avoid attack by the immune system. Although most abnormal cells are efficiently identified and destroyed by the immune system, particularly by NK cells, the molecular mechanisms by which newly born cancer cells evade NK cell surveillance are not fully understood.

Methods: NK cell resistance of highly tumorigenic population of human prostate cancer (PCa) cells were confirmed by xenograft in SCID mice with or without NK cell neutralization. The mechanisms by which the tumorigenic PCa cells evaded NK cell attack were investigated by RNAseq, ChIPseq, generation of several transformants and xenograft in SCID mice.

Results: Here, we show that PCa cells have a strengthened ability to escape NK cell attack due to NANOG, a pluripotent-related transcription factor, mediating the repression of ICAM1, a cell adhesion molecule, during tumorigenesis. Mechanistically, NANOG directly binds to the region upstream of ICAM1. As the binding between NANOG and the upstream ICAM1 region increases, p300 binding to this region is diminished, resulting in decreased ICAM1 expression. High NANOG expression confers PCa cells the ability to resist NK cell attack via the repression of ICAM1. Consistent with these results, low ICAM1 expression is significantly correlated with a high recurrence rate in patients with PCa.

Conclusions: Our findings indicate that repression of ICAM1 is a critical mechanism by which cancer cells evade attack from NK cells during tumorigenesis. These results suggest a pivotal role of NANOG in establishing a gene expression profile for escaping the immune system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13046-019-1429-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796413PMC
October 2019

Neonatal Eating Assessment Tool-Breastfeeding: Reference Values for Infants Less Than 7 Months old.

J Hum Lact 2020 May 29;36(2):236-244. Epub 2019 Aug 29.

2331 University of North Carolina at Chapel Hill School of Nursing, Chapel Hill, NC, USA.

Background: The Neonatal Eating Assessment Tool-Breastfeeding is a valid and reliable 62-item parent-report assessment of symptoms of problematic breastfeeding behavior intended for infants less than 7 months old.

Research Aim: The aim of this study was to describe the Neonatal Eating Assessment Tool-Breastfeeding total score and subscale scores within a sample of full-term, healthy, typically-developing infants under 7 months old.

Methods: Parents of healthy, full-term breastfeeding infants ( = 475) less than 7 months old completed the Neonatal Eating Assessment Tool - Breastfeeding through an online survey. Descriptive statistics were calculated for the total score and seven subscale scores within each age group: 0-2, 2-4, 4-6, and 6-7 months.

Results: Neonatal Eating Assessment Tool-Breastfeeding total scores were highest (i.e., more problematic symptoms) at 0-2 months and decreased in older infant age groups. All subscale scores also had a downward trajectory in symptoms of problematic breastfeeding except the subscale Compelling Symptoms of Problematic Feeding, which was very low across age groups. Scores on the Infant Regulation subscale remained elevated for the first 6 months of life, then declined markedly in the 6-7 month age group.

Conclusion: The Neonatal Eating Assessment Tool-Breastfeeding now has reference values to facilitate interpretation of scores and guide decision-making, personalization of interventions, and assessment of response to interventions. For research, the Neonatal Eating Assessment Tool-Breastfeeding can be used to follow longitudinal development of breastfeeding as well as to test efficacy of breastfeeding interventions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0890334419869598DOI Listing
May 2020

Transformation of 2D Planes into 3D Soft and Flexible Structures with Embedded Electrical Functionality.

ACS Appl Mater Interfaces 2019 Oct 30;11(39):36186-36195. Epub 2019 Aug 30.

Center for BioMicroSystems , Korea Institute of Science and Technology (KIST) , Seoul 02792 , Republic of Korea.

Three-dimensional (3D) structures composed of flexible and soft materials have been in demand for implantable biomedical devices. However, the fabrication of 3D structures using microelectromechanical system (MEMS) techniques has limitations in terms of the materials and the scale of the structures. Here, a technique to selectively bond polydimethylsiloxane (PDMS) and parylene-C by plasma treatment is reported, with which two-dimensional structures that are fabricated using MEMS techniques are turned into 3D structures by the inflation of selectively non-bonded patterns. The bonding strength and the bonding mechanism were analyzed by mechanical tests and chemical analyses, respectively. We fabricated soft and flexible 3D structures with various patterns and dimensions, even with embedded electrical functions, including light emitting diodes and electrocorticogram electrodes. Based on these results, the flexible, soft, and MEMS-capable 3D structures that are obtained by the developed selective bonding technique are promising for applications in a wide range of biomedical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.9b09578DOI Listing
October 2019

Probabilistic ecological risk assessment of heavy metals using the sensitivity of resident organisms in four Korean rivers.

Ecotoxicol Environ Saf 2019 Nov 27;183:109483. Epub 2019 Jul 27.

School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology, 123 Cheomdangwagi-ro, Buk-gu, Gwangju, 61005, Republic of Korea. Electronic address:

The environment has been continuously exposed to heavy metals by various routes, from both natural and artificial sources. In particular, heavy metals in water can affect aquatic organisms adversely, even at very low concentrations, and can lead to the disturbance of the ecosystem balance and biodiversity. Ecological risk assessments are conducted to protect the environment from such situations, primarily by deriving the predicted no-effect concentration (PNEC) from the species sensitivity distribution (SSD). This study developed the SSDs based on the species living in Korean freshwater for four heavy metals including cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn). The species compositions of the SSDs were examined, and three types of PNECs were derived by applying different assessment factors (AF). In addition, the occurrence and concentrations of heavy metals in Korean rivers were investigated, and the ecological risk assessment was carried out to compare the SSDs with the environmental concentrations. The SSDs were developed using a sufficient number of species, but the missing data of plants and insects provided an incomplete species composition. The results show that Cd and Pb in the environmental concentrations of rivers would not cause any risk to aquatic organisms from the derived PNEC. However, some organisms might be adversely affected by the concentrations of Zn, and a small amount of risk was expected under the conservative PNEC. The distribution of Cu in the rivers was not considered to be safe for aquatic organisms because the average environmental concentrations potentially affected the proportion of the SSD, and the environmental concentrations exceeded the PNECs. The concentrations of Cu and Zn in industrial waters indicated a considerable risk to aquatic organisms, and the probability of exceeding the PNECs appeared to be quite high. Therefore, this study indicates that additional actions and parallel field studies are required based on the risk posed to aquatic organisms by Cu and Zn in four Korean rivers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2019.109483DOI Listing
November 2019

Visualization of Iodine Chemisorption Facilitated by Aryl C-H Bond Activation.

ACS Appl Mater Interfaces 2019 Jul 9;11(29):25817-25823. Epub 2019 Jul 9.

Department of Emerging Materials Science , Daegu Gyeongbuk Institute of Science and Technology (DGIST) , 333 Techno Jungang-daero , Dalseong-gun, Daegu 42988 , Korea.

The ability to chemisorb iodine is important for the safe long-term storage of fission products from nuclear reactors. Herein, we successfully used single-crystal X-ray diffraction analysis to crystallographically visualize I binding sites in two isostructural metal-organic frameworks, viz. Co(-DOBDC) (-DOBDC = 4,6-dioxo-1,3-benzenedicarboxylate) and Co(-DOBDC) (-DOBDC = 2,5-dioxo-1,4-benzenedicarboxylate), with increasing I loading. Interestingly, the C-H bond at the electron-rich carbon (C5) of -DOBDC is activated toward electrophilic aromatic substitution, forming an aryl C-I bond and I or I that coordinates to unsaturated open Co sites. Cooperation between the ligand and the open Co sites leads to rapid chemisorption of I even under mild adsorption conditions, such as room temperature. In contrast, molecular I coordinates to the open Co sites of Co(-DOBDC). Owing to the chemisorption of I, [email protected](-DOBDC) decomposes at a much higher temperature than [email protected](-DOBDC), as revealed by thermogravimetric analysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.9b04768DOI Listing
July 2019

Histone H3 lysine 4 methyltransferase is required for facultative heterochromatin at specific loci.

BMC Genomics 2019 May 8;20(1):350. Epub 2019 May 8.

Department of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA.

Background: Histone H3 lysine 4 tri-methylation (H3K4me3) and histone H3 lysine 9 tri-methylation (H3K9me3) are widely perceived to be opposing and often mutually exclusive chromatin modifications. However, both are needed for certain light-activated genes in Neurospora crassa (Neurospora), including frequency (frq) and vivid (vvd). Except for these 2 loci, little is known about how H3K4me3 and H3K9me3 impact and contribute to light-regulated gene expression.

Results: In this report, we performed a multi-dimensional genomic analysis to understand the role of H3K4me3 and H3K9me3 using the Neurospora light response as the system. RNA-seq on strains lacking H3 lysine 4 methyltransferase (KMT2/SET-1) and histone H3 lysine 9 methyltransferase (KMT1/DIM-5) revealed some light-activated genes had altered expression, but the light response was largely intact. Comparing these 2 mutants to wild-type (WT), we found that roughly equal numbers of genes showed elevated and reduced expression in the dark and the light making the environmental stimulus somewhat ancillary to the genome-wide effects. ChIP-seq experiments revealed H3K4me3 and H3K9me3 had only minor changes in response to light in WT, but there were notable alterations in H3K4me3 in Δkmt1/Δdim-5 and H3K9me3 in Δkmt2/Δset-1 indicating crosstalk and redistribution between the modifications. Integrated analysis of the RNA-seq and ChIP-seq highlighted context-dependent roles for KMT2/SET1 and KMT1/DIM-5 as either co-activators or co-repressors with some overlap as co-regulators. At a small subset of loci, H3K4 methylation is required for H3K9me3-mediated facultative heterochromatin including, the central clock gene frequency (frq). Finally, we used sequential ChIP (re-ChIP) experiment to confirm Neurospora contains K4/K9 bivalent domains.

Conclusions: Collectively, these data indicate there are obfuscated regulatory roles for H3K4 methylation and H3K9 methylation depending on genome location with some minor overlap and co-dependency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12864-019-5729-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505117PMC
May 2019
-->