Publications by authors named "Jingyu Wang"

434 Publications

Field-Created Coordinate Cation Bridges Enable Conductance Modulation and Artificial Synapse within Metal Nanoparticles.

Nano Lett 2022 Aug 8. Epub 2022 Aug 8.

CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, People's Republic of China.

When metal nanoparticles are functionalized with charged ligands, the movement of counterions and conduction electrons is coupled, which enables us to develop electronic devices, including diodes, transistors, and logic gates, but dynamically modulating the conductivity of a synaptic device within these materials has proved challenging. Here we show that an artificial synapse can be created from thin films of functionalized metal nanoparticles using an active silver electrode. The electric-field-injected Ag coordinates with carboxyl ligands that sets up a conduction bridge to increase the nanoparticle conductivity by reducing the electron tunneling/hopping energy barriers. The dynamic modulation of conductivity allows us to implement several important synaptic functions such as potentiation/depression, paired-pulse facilitation, learning behaviors including short-term to long-term memory transition, self-learning, and massed leaning vs spaced learning. Finally, based on the nonvolatile characteristics, the metal nanoparticle synapse is used to build a single-layer hardware spiking neural network (SNN) for pattern recognition.
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http://dx.doi.org/10.1021/acs.nanolett.2c02675DOI Listing
August 2022

Research status of gas sensing performance of MoTe-based gas sensors: A mini review.

Front Chem 2022 22;10:950974. Epub 2022 Jul 22.

College of Engineering and Technology, Southwest University, Chongqing, China.

Transition metal dichalcogenides (TMDs) have been widely explored for their excellent gas sensing properties, especially high sensitivity and stability at room temperature. MoTe exhibits good sensitivity and selectivity to some nitrogen-containing gases (i.e., NO, NH) and has received extensive attention in gas sensing. In addition, increasingly complex production environments place demands on high-quality gas sensors. Therefore, worldwide efforts are devoted to designing and manufacturing MoTe-based gas sensors with faster response and recovery speed. This paper summarizes the research progress of MoTe-based gas sensing, focuses on the practical measures to improve the response and recovery speed of MoTe-based sensors, and discusses the mechanism. This provides guidance for exploring higher performance MoTe sensors.
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http://dx.doi.org/10.3389/fchem.2022.950974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354782PMC
July 2022

Macrophages induce cardiomyocyte ferroptosis via mitochondrial transfer.

Free Radic Biol Med 2022 Aug 3;190:1-14. Epub 2022 Aug 3.

Department of Basic Medicine Sciences, And Department of Orthopaedics of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China. Electronic address:

Introduction: Mitochondrial transfer is a new cell-to-cell communication manner. Whether the mitochondrial transfer is also involved in the macrophage infiltration-induced cardiac injury is unclear.

Objectives: This study aimed to determine whether macrophage mitochondria can be transferred to cardiomyocytes, and to investigate its possible role and mechanism.

Methods: Mitochondrial transfer between macrophages and cardiomyocytes was detected using immunofluorescence staining and flow cytometry. Cellular metabolites were analyzed using LC-MS technique. Differentially expressed mRNAs were identified using RNA-seq technique.

Results: (1) After cardiomyocytes were cultured with macrophage-conditioned medium (COND + group), macrophage-derived mitochondria have been found in cardiomyocytes, which could be blocked by dynasore (an inhibitor of clathrin-mediated endocytosis). (2) Compared with control (CM) group, there were 545 altered metabolites found in COND + group, most of which were lipids and lipid-like molecules. The altered metabolites were mainly enriched in the β-oxidation of fatty acids and glutathione metabolism. And there were 4824 differentially expressed mRNAs, which were highly enriched in processes like lipid metabolism-associated pathway. (3) Both RNA-seq and qRT-PCR results found that ferroptosis-related mRNAs such as Ptgs2 and Acsl4 increased, and Gpx4 mRNA decreased in COND + group (P < 0.05 vs CM group). (4) The levels of cellular free Fe and mitochondrial lipid peroxidation were increased; while GSH/GSSG ratio, mitochondrial aspect ratio, mitochondrial membrane potential, and ATP production were decreased in cardiomyocytes of COND + group (P < 0.05 vs CM group). All the above phenomena could be blocked by a ferroptosis inhibitor ferrostatin-1 (P < 0.05).

Conclusion: Macrophages could transfer mitochondria to cardiomyocytes. Macrophage-derived mitochondria were internalized into cardiomyocytes through clathrin- and/or lipid raft-mediated endocytosis. Uptake of exogenous macrophage mitochondria induced cardiomyocyte injury via triggering ferroptosis.
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http://dx.doi.org/10.1016/j.freeradbiomed.2022.07.015DOI Listing
August 2022

Resveratrol suppresses microglial activation and promotes functional recovery of traumatic spinal cord via improving intestinal microbiota.

Pharmacol Res 2022 Aug 1;183:106377. Epub 2022 Aug 1.

Department of Basic Medicine Sciences, and Department of Orthopaedics of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Spinal cord injury (SCI) can change the intestinal microbiota pattern and corresponding metabolites, which in turn affect the prognosis of SCI. Among many metabolites, short-chain fatty acids (SCFAs) are critical for neurological recovery after SCI. Recent research has shown that resveratrol exerts anti-inflammatory properties. But it is unknown if the anti-inflammatory properties of resveratrol are associated with intestinal microbiota and metabolites. We thus investigate the alteration in gut microbiota and the consequent change of SCFAs following resveratrol treatment. The SCI mouse models with retention of gut microbiota (donor) and depletion of gut microbiota (recipient) were established. Fecal microbiota transplantation from donors to recipients was performed with intragastrical administration. Spinal cord tissues of mice were examined by H&E, Nissl, and immunofluorescence stainings. The expressions of the inflammatory profile were examined by qPCR and cytometric bead array. Fecal samples of mice were collected and analyzed with 16S rRNA sequencing. The results showed that resveratrol inhibited the microglial activation and promoted the functional recovery of SCI. The analysis of intestinal microbiota and metabolites indicated that SCI caused dysbiosis and the decrease in butyrate, while resveratrol restored microbiota pattern, reversed intestinal dysbiosis, and increased the concentration of butyrate. Both fecal supernatants from resveratrol-treated donors and butyrate suppressed the expression of pro-inflammatory genes in BV2 microglia. Our result demonstrated that fecal microbiota transplantation from resveratrol-treated donors had beneficial effects on the functional recovery of SCI. One mechanism of resveratrol effects was to restore the disrupted gut microbiota and butyrate.
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http://dx.doi.org/10.1016/j.phrs.2022.106377DOI Listing
August 2022

Machine learning-based inverse design for electrochemically controlled microscopic gradients of O and HO.

Proc Natl Acad Sci U S A 2022 Aug 1;119(32):e2206321119. Epub 2022 Aug 1.

Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095.

A fundamental understanding of extracellular microenvironments of O and reactive oxygen species (ROS) such as HO, ubiquitous in microbiology, demands high-throughput methods of mimicking, controlling, and perturbing gradients of O and HO at microscopic scale with high spatiotemporal precision. However, there is a paucity of high-throughput strategies of microenvironment design, and it remains challenging to achieve O and HO heterogeneities with microbiologically desirable spatiotemporal resolutions. Here, we report the inverse design, based on machine learning (ML), of electrochemically generated microscopic O and HO profiles relevant for microbiology. Microwire arrays with suitably designed electrochemical catalysts enable the independent control of O and HO profiles with spatial resolution of ∼10 μm and temporal resolution of ∼10° s. Neural networks aided by data augmentation inversely design the experimental conditions needed for targeted O and HO microenvironments while being two orders of magnitude faster than experimental explorations. Interfacing ML-based inverse design with electrochemically controlled concentration heterogeneity creates a viable fast-response platform toward better understanding the extracellular space with desirable spatiotemporal control.
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http://dx.doi.org/10.1073/pnas.2206321119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371721PMC
August 2022

A Dual-Branch Self-Boosting Framework for Self-Supervised 3D Hand Pose Estimation.

IEEE Trans Image Process 2022 2;31:5052-5066. Epub 2022 Aug 2.

Although 3D hand pose estimation has made significant progress in recent years with the development of the deep neural network, most learning-based methods require a large amount of labeled data that is time-consuming to collect. In this paper, we propose a dual-branch self-boosting framework for self-supervised 3D hand pose estimation from depth images. First, we adopt a simple yet effective image-to-image translation technology to generate realistic depth images from synthetic data for network pre-training. Second, we propose a dual-branch network to perform 3D hand model estimation and pixel-wise pose estimation in a decoupled way. Through a part-aware model-fitting loss, the network can be updated according to the fine-grained differences between the hand model and the unlabeled real image. Through an inter-branch loss, the two complementary branches can boost each other continuously during self-supervised learning. Furthermore, we adopt a refinement stage to better utilize the prior structure information in the estimated hand model for a more accurate and robust estimation. Our method outperforms previous self-supervised methods by a large margin without using paired multi-view images and achieves comparable results to strongly supervised methods. Besides, by adopting our regenerated pose annotations, the performance of the skeleton-based gesture recognition is significantly improved.
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http://dx.doi.org/10.1109/TIP.2022.3192708DOI Listing
August 2022

Response mechanism of Vibrio parahaemolyticus at high pressure revealed by transcriptomic analysis.

Appl Microbiol Biotechnol 2022 Jul 25. Epub 2022 Jul 25.

College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, Shandong, China.

Vibrio parahaemolyticus is a common pathogen in aquatic products, such as shellfishes. Laboratory-based simulated studies demonstrated that V. parahaemolyticus can tolerate high hydrostatic pressure (HHP) up to 20 MPa. However, the molecular mechanisms of high-pressure adaptation remain unclear. Herein, we analyzed the physiological changes and transcriptomic responses of V. parahaemolyticus ATCC 17,802 under HHP conditions to determine the possible survival mechanisms. Under HHP conditions, the morphology of V. parahaemolyticus was notably changed exhibiting the coccoid microbial cells. The transcriptome analysis revealed that there were 795 differentially expressed genes (DEGs) under the 20 MPa condition, including 406 upregulated DEGs and 389 downregulated DEGs. Most of the downregulated DEGs encoded proteins related to energy metabolism, such as citrate synthase (gltA), pyruvate kinase (pyk), and glyceraldehyde-3-phosphate dehydrogenase (gapA). Many of the upregulated DEGs encoded proteins related to adhesion and virulence factors, such as RNA polymerase σ factor (rpoE), L-threonine 3-dehydrogenase, and bacterial nucleotide signal c-di-GMP (WU75_RS02745 and WU75_RS07185). In our proposed mechanism model, V. parahaemolyticus responds to HHP stress through RNA polymerase σ factor RpoE. These findings indicate that V. parahaemolyticus cells may adopt a complex adaptation strategy to cope with HHP stress. KEY POINTS: •The transcriptomic response of Vibrio parahaemolyticus under HHP conditions was studied for the first time. •V. parahaemolyticus may adopt a complex adaptation strategy to cope with HHP stress. •ToxRS and RpoE played an important role in sensing and responding the HHP signal.
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http://dx.doi.org/10.1007/s00253-022-12082-yDOI Listing
July 2022

A systematic review of deep learning methods for modeling electrocardiograms during sleep.

Physiol Meas 2022 Aug 12;43(8). Epub 2022 Aug 12.

School of Artificial Intelligence, Peking University, Beijing, 100871, People's Republic of China.

Sleep is one of the most important human physiological activities, and plays an essential role in human health. Polysomnography (PSG) is the gold standard for measuring sleep quality and disorders, but it is time-consuming, labor-intensive, and prone to errors. Current research has confirmed the correlations between sleep and the respiratory/circulatory system. Electrocardiography (ECG) is convenient to perform, and ECG data are rich in breathing information. Therefore, sleep research based on ECG data has become popular. Currently, deep learning (DL) methods have achieved promising results on predictive health care tasks using ECG signals. Therefore, in this review, we systematically identify recent research studies and analyze them from the perspectives of data, model, and task. We discuss the shortcomings, summarize the findings, and highlight the potential opportunities. For sleep-related tasks, many ECG-based DL methods produce more accurate results than traditional approaches by combining multiple signal features and model structures. Methods that are more interpretable, scalable, and transferable will become ubiquitous in the daily practice of medicine and ambient-assisted-living applications. This paper is the first systematic review of ECG-based DL methods for sleep tasks.
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http://dx.doi.org/10.1088/1361-6579/ac826eDOI Listing
August 2022

Associations of essential element serum concentrations with autism spectrum disorder.

Environ Sci Pollut Res Int 2022 Jul 16. Epub 2022 Jul 16.

Neuroscience Research Institute, Peking University, Beijing, 100191, China.

This case-control study explored the associations between autism spectrum disorder (ASD) and the serum concentration of nine chemical elements in children. The study recruited 92 Chinese children with ASD and 103 typically developing individuals. Serum concentrations of nine chemical elements (calcium, iodine, iron, lithium, magnesium, potassium, selenium, strontium, and zinc) were determined by inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma atomic emission spectrometry (ICP-AES). An unconditional logistic regression model was used to analyze the associations between the serum concentrations of the elements and the risk of ASD. After adjusting for confounders, the multivariate analysis results showed that zinc ≤ 837.70 ng/mL, potassium > 170.06 μg/mL, and strontium ≤ 52.46 ng/mL were associated with an increased risk of ASD, while selenium > 159.80 ng/mL was associated with a decreased risk of ASD. Furthermore, the degree of lithium and zinc deficiency was associated with ASD severity. The results indicated that metallomic profiles of some specific elements might play important roles in the development of ASD, a finding of scientific significance for understanding the etiology, and providing dietary guidance for certain ASD types.
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http://dx.doi.org/10.1007/s11356-022-21978-1DOI Listing
July 2022

The Effector BspI Suppresses Inflammation via Inhibition of IRE1 Kinase Activity during Infection.

J Immunol 2022 Aug 15;209(3):488-497. Epub 2022 Jul 15.

Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, China;

Mammalian GTPase-activating proteins (GAPs) can inhibit innate immunity signaling in a spatiotemporal fashion; however, the role of bacterial GAPs in mediating innate immunity remains unknown. In this study, we show that BspI, a type IV secretion system (T4SS) effector protein, containing a GAP domain at the C terminus, negatively regulates proinflammatory responses and host protection to infection in a mouse model. In macrophages, BspI inhibits the activation of inositol-requiring enzyme 1 (IRE1) kinase, but it does not inhibit activation of ATF6 and PERK. BspI suppresses induction of proinflammatory cytokines via inhibiting the activity of IRE1 kinase caused by VceC, a type IV secretion system effector protein that localizes to the endoplasmic reticulum. Ectopically expressed BspI interacts with IRE1 in HeLa cells. The inhibitory function of BspI depends on its GAP domain but not on interaction with small GTPase Ras-associated binding protein 1B (RAB1B). Collectively, these data support a model where BspI, in a GAP domain-dependent manner, inhibits activation of IRE1 to prevent proinflammatory cytokine responses.
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http://dx.doi.org/10.4049/jimmunol.2200001DOI Listing
August 2022

Broadband photodetector based on SnTe nanofilm/n-Ge heterostructure.

Nanotechnology 2022 Jul 29;33(42). Epub 2022 Jul 29.

Kunming Institute of Physics, Kunming 650223, People's Republic of China.

Combining novel two-dimensional materials with traditional semiconductors to form heterostructures for photoelectric detection have attracted great attention due to their excellent photoelectric properties. In this study, we reported the formation of a heterostructure comprising of tin telluride (SnTe) and germanium (Ge) by a simple and efficient one-step magnetron sputtering technique. A photodetector was fabricated by sputtering a nanofilm of SnTe on to a pre-masked n-Ge substrate.-measurements obtained from the SnTe/n-Ge photodetector demonstrated diode and photovoltaic characteristics in the visible to near-infrared (NIR) band (i.e. 400-2050 nm). Under NIR illumination at 850 nm with an optical power density of 13.81 mW cm, the SnTe/n-Ge photodetector exhibited a small open-circuit voltage of 0.05 V. It also attained a high responsivity () and detectivity (*) of 617.34 mA W(at bias voltage of -0.5 V) and 2.33 × 10cmHzW(at zero bias), respectively. Therefore, SnTe nanofilm/n-Ge heterostructure is highly suitable for used as low-power broadband photodetector due to its excellent performances and simple device configuration.
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http://dx.doi.org/10.1088/1361-6528/ac80ccDOI Listing
July 2022

Two Solvent-Induced In(III)-Based Metal-Organic Frameworks with Controllable Topology Performing High-Efficiency Separation of CH/CH and CO/CH.

Inorg Chem 2022 Jul 11;61(29):11057-11065. Epub 2022 Jul 11.

Institute of Molecular Sciences and Engineering, Shandong University, Qingdao 266237, P. R. China.

For pure acetylene manufacturing and natural gas purification, the development of porous materials displaying highly selective CH/CH and CO/CH separation is greatly important but remains a major challenge. In this work, a plausible strategy involving solvent-induced effects and using the flexibility of the ligand conformation to make two In(III) metal-organic frameworks (MOFs) is developed, showing topological diversity and different stability. The X-shaped tetracarboxylic ligand HTPTA ([1,1':3',1″-terphenyl]-4,4',4″,6'-tetracarboxylic acid) was selected to construct two new heteroid In MOFs, namely, {[CHNH][In(TPTA)]·2(NMF)} (MOF 1) and {[In(TPTA)(OH)]·2(HO)·(DMF)} (MOF 2). MOF 1 is a (4, 4)-connected net showing a topology with a large channel that is not conducive to fine gas separation. By contrast, with the reduction of SBU from uninucleated In to an {In-OH-In} chain, MOF 2 has a (4, 6)-connected net with the topology with an ∼5 Å suitable micropore to confine matching small gas. The permanent porosity of MOF 2 leads to the preferential adsorption of CH over CO with superior CH/CH (332.3) and CO/CH (31.2) separation selectivities. Meanwhile, the cycling dynamic breakthrough experiments showed that the high-purity CH (>99.8%) capture capacities of MOF 2 were >1.92 mmol g from a binary CH/CH mixture, and its separation factor reached 10.
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http://dx.doi.org/10.1021/acs.inorgchem.2c00694DOI Listing
July 2022

Effect Analysis of Preoperative Intravenous Tranexamic Acid Combined With Intraoperative Immersion in Reducing Perioperative Blood Loss of One Stage Posterior Thoracolumbar Tuberculosis.

Front Surg 2022 23;9:852589. Epub 2022 Jun 23.

Department of Spine Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.

Background: To investigate the efficacy and safety of preoperative intravenous tranexamic acid (TXA) combined with intraoperative immersion in reducing perioperative blood loss in one-stage posterior thoracolumbar tuberculosis.

Methods: All patients were divided into four groups: Group A received an intravenous drip of TXA before surgery, group B received multiple local immersions during the operation, group C received an intravenous drip combined with multiple local immersions, and the control group (group CG) were not treated with TXA during the same period. The total blood loss (TBL), intraoperative blood loss (IBL), hidden blood loss (HBL), postoperative drainage volume, maximum hemoglobin drop value (max Hb drop), liver and kidney function, coagulation indexes, blood transfusion rate, hospital stay and incidence of complications were compared among the four groups.

Results: TBL, IBL, HBL, max Hb drop, POD1 drainage, and POD2 drainage in group A, group B, and group C were significantly lower than those in group CG. TBL, IBL, HBL and max Hb drop were group C < group A < group B < group CG. The drainage volume of group C was significantly lower than that of the other groups. There was no significant difference in blood coagulation index (PT, D-D) or liver and kidney function (ALT, Cr) among the four groups. There was no difference in postoperative hospital stay between group A and group B, but it was significantly lower in group C than in the other three groups. All patients achieved satisfactory bone graft fusion at the last follow-up.

Conclusion: Preoperative intravenous drip of TXA combined with intraoperative multiple immersion can effectively reduce perioperative blood loss while not increasing the risk of thrombosis without affecting liver and kidney function, coagulation function or tuberculosis prognosis.
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http://dx.doi.org/10.3389/fsurg.2022.852589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260174PMC
June 2022

Transcriptome Analysis of Retinoic Acid-Inducible Gene I Overexpression Reveals the Potential Genes for Autophagy-Related Negative Regulation.

Cells 2022 06 23;11(13). Epub 2022 Jun 23.

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Retinoic acid-inducible gene I (RIG-I) serves as an essential viral RNA sensor for innate immune. The activation of the RIG-I-like receptors (RLRs) pathway triggers many regulations for the outcome of type I interferon, including ubiquitination, dephosphorylation, ISGylation, and autophagy. However, the autophagy-related regulation of RIG-I is still not fully understood. To investigate the potentially unknown genes related to autophagy-related regulation of RIG-I, we firstly confirm the induction of autophagy derived by overexpression of RIG-I. Furthermore, the autophagy inducer and inhibitor drugs were used in different assays. The results showed autophagy could control the activation of RLRs pathway and expression of exogenous RIG-I. In addition, we carried out the transcriptome analysis of overexpression of RIG-I in vitro. Differentially expressed genes (DEGs) in GO and KEGG signaling pathways enrichment provided a newly complex network. Finally, the validation of qPCR indicated that the DEGs PTPN22, PRKN, OTUD7B, and SIRT2 were correlated to the negative regulation of excessive expression of RIG-I. Taken together, our study contributed new insights into a more comprehensive understanding of the regulation of excessive expression of RIG-I. It provided the potential candidate genes for autophagy-related negative regulation for further investigation.
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http://dx.doi.org/10.3390/cells11132009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265583PMC
June 2022

Bidirectional Effects Between Parental Care and Depression Among Adolescent Boys: Results From the Chinese Family Panel Studies.

Front Psychol 2022 15;13:803450. Epub 2022 Jun 15.

Department of Communication, University of Arizona, Tucson, AZ, United States.

Background: Research has consistently shown the adverse effects of inappropriate parenting on adolescent depression. Meanwhile, interpersonal theories of depression suggest that depressed individuals elicit frustration and rejection from their relational partners.

Method: Using two-wave data from the Chinese Family Panel Studies (CFPS), the present study examined the prospective relationships between parental care and adolescent depression. Participant were 426 adolescents (54.9% male) born in 1999 (ages at T1 and T2 were 11 and 13).

Results: Results from the structural equation model showed that parental care prospectively and negatively predicted depression among both adolescent boys and girls. Inversely, adolescent boys' depression, but not girls' depression, negatively predicted subsequent parental care.

Conclusion: The results suggest the interactive dynamic between parental care and adolescent depression as well as parents' gendered responses to adolescent depression.
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http://dx.doi.org/10.3389/fpsyg.2022.803450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240277PMC
June 2022

Associations of the Cardiometabolic Index with the Risk of Cardiovascular Disease in Patients with Hypertension and Obstructive Sleep Apnea: Results of a Longitudinal Cohort Study.

Oxid Med Cell Longev 2022 23;2022:4914791. Epub 2022 Jun 23.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Hypertension Institute, National Health Committee Key Laboratory of Hypertension Clinical Research, Key Laboratory of Xinjiang Uygur Autonomous Region, Xinjiang Clinical Medical Research Center for Hypertension Diseases, Urumqi, China.

Purpose: We aimed to explore the relationship between the cardiometabolic index (CMI) and cardiovascular disease (CVD) and its subtypes (coronary artery disease and stroke) in patients with hypertension and obstructive sleep apnea (OSA).

Methods: We conducted a retrospective cohort study enrolling 2067 participants from the Urumqi Research on Sleep Apnea and Hypertension study. The CMI was calculated as triglyceride to high-density lipoprotein cholesterol ratio × waist-to-height ratio. Participants were divided into three groups (T1, T2, and T3) according to the tertile of CMI. The Kaplan-Meier method helped to calculate the cumulative incidence of CVD in different groups. We assessed the association of CMI with the risk of CVD and CVD subtypes by estimating hazard ratios (HRs) and 95% confidence intervals (CIs) using Cox models.

Results: During a median follow-up of 6.83 years (interquartile range: 5.92-8.00 years), 326 incident CVD were identified, including 121 incident stroke and 205 incident coronary heart disease (CHD). Overall, after adjusting for confounding variables, CMI was positively associated with the risk of new-onset CVD (per SD increment, adjusted HR: 1.31; 95% CI: 1.20, 1.43), new-onset CHD (per SD increment, adjusted HR: 1.33; 95% CI: 1.20, 1.48), and new-onset stroke (per SD increment, adjusted HR: 1.27; 95% CI: 1.10, 1.47). Similar results were obtained in various subgroup and sensitivity analyses. Adding CMI to the baseline risk model for CVD improved the -index ( < 0.001), continuous net reclassification improvement ( < 0.001), and integrated discrimination index ( < 0.001). Similar results were observed for CHD and stroke.

Conclusion: There was a positive association between CMI levels and the risk of new-onset CVD in patients with hypertension and OSA. This finding suggests that CMI may help identify people at high risk of developing CVD.
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http://dx.doi.org/10.1155/2022/4914791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246614PMC
July 2022

The effect of ultrasound-guided lung recruitment maneuvers on atelectasis in lung-healthy patients undergoing laparoscopic gynecologic surgery: a randomized controlled trial.

BMC Anesthesiol 2022 07 1;22(1):200. Epub 2022 Jul 1.

Department of Anesthesiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, Sichuan Province, China.

Background: Atelectasis is the primary cause of hypoxemia during general anesthesia. This study aimed to evaluate the impact of the combination of recruitment maneuvers (RM) and positive end-expiratory pressure (PEEP) on the incidence of atelectasis in adult women undergoing gynecologic laparoscopic surgery using pulmonary ultrasound.

Methods: In this study, 42 patients with healthy lungs undergoing laparoscopic gynecologic surgery were randomly divided into the recruitment maneuver group (RM group; 6 cm HO PEEP and RM) or the control group (C group; 6 cm HO PEEP and no RM), 21 patients in each group. Volume-controlled ventilation was used in all selected patients, with a tidal volume of 6-8 mL·kg of ideal body weight. When atelectasis was detected, patients in the RM group received ultrasound-guided RM, while those in the C group received no intervention. The incidence and severity of atelectasis were determined using lung ultrasound scores.

Results: A total of 41 patients were investigated. The incidence of atelectasis was lower in the RM group (40%) than in the C group (80%) 15 min after arrival in the post-anesthesia care unit (PACU). Meanwhile, lung ultrasound scores (LUSs) were lower in the RM group compared to the C group. In addition, the differences in the LUS between the two groups were mainly due to the differences in lung ultrasound scores in the posterior regions. However, this difference did not persist after 24 h of surgery.

Conclusions: In conclusion, the combination of RM and PEEP could reduce the incidence of atelectasis in patients with healthy lungs 15 min after arrival at the PACU; however, it disappeared within 24 h after surgery.

Trial Registration: (Prospectively registered): ChiCTR2000033529 . Registered on 4/6/2020.
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http://dx.doi.org/10.1186/s12871-022-01742-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248140PMC
July 2022

The relationship between bladder storage function and frequent micturition after TURP.

World J Urol 2022 Aug 1;40(8):2055-2062. Epub 2022 Jul 1.

Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, 030032, China.

Purpose: To investigate the relationship between preoperative bladder function and frequent micturition after transurethral resection of prostate in patients with benign prostatic hyperplasia.

Methods: We retrospectively included 80 eligible patients aged 54-87 years (mean age 69.8 years) who underwent transurethral resection of the prostate at our hospital from January 2019 to October 2021. Patients were divided into detrusor overactivity positive and negative groups, and according to bladder compliance, they were divided into: low (G1), normal (G2), and high (G3) bladder compliance groups.

Results: The incidence and score of postoperative frequent micturition in the detrusor overactivity positive group were higher than those in the detrusor overactivity negative group. The incidence and score of postoperative frequent micturition in the low bladder compliance group were higher than those in the normal and high bladder compliance groups. There was no significant difference in the score of frequent micturition between the normal and high bladder compliance groups. Multivariate logistic regression analysis indicated that frequent micturition was significantly correlated with detrusor overactivity, bladder compliance, maximum cystometric capacity, and maximum flow rate.

Conclusion: This study confirmed that patients with abnormal bladder storage functions (detrusor instability and low bladder compliance) before transurethral resection of the prostate were likely to have frequent and severe urination after transurethral resection of the prostate. Therefore, preoperative urodynamic examination to evaluate the urinary storage function of patients with benign prostatic hyperplasia is of great significance to predict the occurrence and degree of postoperative frequent micturition.
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http://dx.doi.org/10.1007/s00345-022-04079-zDOI Listing
August 2022

Immune Deviation in the Decidua During Term and Preterm Labor.

Front Immunol 2022 10;13:877314. Epub 2022 Jun 10.

Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The maternal-fetal immune disorder is considered to be an important factor of preterm birth (PTB); however, the underlying mechanism is still not fully understood. This study was designed to explore the innate and adaptive immune features in the decidua during term and preterm labor. Women delivered at term or preterm were classified into four groups: term not in labor (TNL, N=19), term in labor (TL, N=17), preterm not in labor (PNL, N=10), and preterm in labor (PIL, N=10). Decidua basalis and parietalis were collected and analyzed for macrophage subtypes (M1 and M2) as well as T helper 1 (Th1), Th2, Th17 and regulatory T (Treg) cells by flow cytometry and immunohistochemistry. Our results demonstrated significantly decreased frequencies of M2 cells and elevated M1/M2 ratio in the PIL group compared to that in the PNL group in both decidua basalis and parietalis, whereas no significant differences were found between the above two groups in both sites in terms of the polarization status of Th cells. On the contrary, macrophage subsets were comparable in the TL and TNL groups, whereas elevated Th1 percentages and Th1/Th2 ratio were observed in TL women compared to that in TNL women in the decidua. Interestingly, although the frequencies and ratios of Th17 and Treg were comparable among the four groups, the Th17/Treg ratios of these groups were significantly increased in decidua basalis than that in decidua parietalis. Collectively, the M1/M2 imbalance is associated with the breakdown of maternal-fetal immune tolerance during PTB, whereas the aberrant Th1/Th2 profile plays an important role in immune disorder during term labor. Moreover, Th17/Treg deviation is more remarkable in decidua basalis than in decidua parietalis.
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http://dx.doi.org/10.3389/fimmu.2022.877314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226582PMC
June 2022

Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.

Curr Issues Mol Biol 2022 Jun 20;44(6):2759-2771. Epub 2022 Jun 20.

School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.

Parkin is a well-established synergistic mediator of mitophagy in dysfunctional mitochondria. Mitochondria are the main target of arsenic trioxide (ATO) cytotoxicity, and the effect of mitophagy on ATO action remains unclear. In this study, we used stable Parkin-expressing (YFP-Parkin) and Parkin loss-of-function mutant (Parkin C431S) HeLa cell models to ascertain whether Parkin-mediated mitophagy participates in ATO-induced apoptosis/cell death. Our data showed that the overexpression of Parkin significantly sensitized HeLa cells to ATO-initiated proliferation inhibition and apoptosis; however, the mutation of Parkin C431S significantly weakened this Parkin-mediated responsiveness. Our further investigation found that ATO significantly downregulated two fusion proteins (Mfn1/2) and upregulated fission-related protein (Drp1). Autophagy was also activated as evidenced by the formation of autophagic vacuoles and mitophagosomes, increased expression of PINK1, and recruitment of Parkin to impaired mitochondria followed by their degradation, accompanied by the increased transformation of LC3-I to LC3-II, increased expression of Beclin1 and decreased expression of P62 in YFP-Parkin HeLa cells. Enhanced mitochondrial fragmentation and autophagy indicated that mitophagy was activated. Furthermore, during the process of mitophagy, the overproduction of ROS implied that ROS might represent a key factor that initiates mitophagy following Parkin recruitment to mitochondria. In conclusion, our findings indicate that Parkin is critically involved in ATO-triggered mitophagy and functions as a potential antiproliferative target in cancer cells.
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http://dx.doi.org/10.3390/cimb44060189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222214PMC
June 2022

Investigation on the Control of COVID-19 in Wuhan: Number of Infections Outside Hospitals and the Reproduction Number.

Disaster Med Public Health Prep 2022 Jun 21:1-16. Epub 2022 Jun 21.

College of Computer and Information Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

COVID-19 is erupting globally, and Wuhan successfully controlled it within a month. Infections arose from infectious persons outside hospitals. After data revision, data-based and model-based analyses were implemented, and the conclusions are as follows. The incubation period of most infected people may be 6-7 days. The number of infectious persons outside hospitals in Wuhan on January 20, 2020 was about 10000 and reached more than 20000 on the day of Lockdown; it exceeded 72000 on February 4. Both data-based and model-based analyses gave out the evolution of the reproduction number, which was over 2.5 in early January, went down to 1.62 in late January and 1.20 in early February, with a sudden drop to less than 0.5 due to the strict Stay-at-home management after February 11. Strategies of Stay-at-home, Safe-protective measures, and Ark hospitals were the main contributions to control COVID-19 in Wuhan. In Wuhan, 2 inflection points of COVID-19, exactly correspond to February 5 and February 15, the 2 days when Ark hospitals were introduced, and the complete implementation of Stay-at-home. Based on the expression of the reproduction number, group immunity is also discussed. It shows that only when the group immunization rate is over 75% can COVID-19 be under control; group immunity would be full infection and the total deaths will be 220000 for a city as big as Wuhan. Sensitivity analysis suggests that 30% of people staying at home in combination with better behavior changes, such as social-distancing and frequent handwashing, can effectively contain COVID-19. However, only when this proportion is over 60% can the controlled effect and efficiency like Wuhan be obtained.
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http://dx.doi.org/10.1017/dmp.2022.161DOI Listing
June 2022

Dynamic Phycobilin Pigment Variations in Diazotrophic and Non-diazotrophic Cyanobacteria Batch Cultures Under Different Initial Nitrogen Concentrations.

Front Microbiol 2022 2;13:850997. Epub 2022 Jun 2.

The Institute of Ecological, Earth & Environmental Sciences, Baylor University, Waco, TX, United States.

Increased anthropogenic nutrient loading has led to eutrophication of aquatic ecosystems, which is the major cause of harmful cyanobacteria blooms. Element stoichiometry of cyanobacteria bloom is subject to nutrient availabilities and may significantly contribute to primary production and biogeochemical cycling. Phycobilisome is the antenna of the photosynthetic pigment apparatus in cyanobacteria, which contains phycobilin pigments (PBPs) and linker proteins. This nitrogen (N)-rich protein complex has the potential to support growth as a N-storage site and may play a major role in the variability of cyanobacteria N stoichiometry. However, the regulation of PBPs during bloom formation remains unclear. We investigated the temporal variation of N allocation into PBPs and element stoichiometry for two ubiquitous cyanobacteria species, and , in a batch culture experiment with different initial N availabilities. Our results indicated that the N allocation into PBPs is species-dependent and tightly regulated by the availability of nutrients fueling population expansion. During the batch culture experiment, different nutrient uptake rates led to distinct stoichiometric imbalances of N and phosphorus (P), which substantially altered cyanobacteria C: N and C: P stoichiometry. invested cellular N into PBPs and exhibited greater flexibility in C: N and C: P stoichiometry than . The dynamics of such N-rich macromolecules may help explain the N stoichiometry variation during a bloom and the interspecific difference between and . Our study provides a quantitative understanding of the elemental stoichiometry and the regulation of PBPs for non-diazotrophic and diazotrophic cyanobacteria blooms.
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http://dx.doi.org/10.3389/fmicb.2022.850997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201475PMC
June 2022

The role and interaction between transcription factor NAC-NOR and DNA demethylase SlDML2 in the biosynthesis of tomato fruit flavor volatiles.

New Phytol 2022 Sep 30;235(5):1913-1926. Epub 2022 Jun 30.

Laboratory of Fruit Quality Biology/Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.

Flavor-imparting volatile chemicals accumulate as fruits ripen, making major contributions to taste. The NAC transcription factor nonripening (NAC-NOR) and DNA demethylase 2 (SlDML2) are essential for tomato fruit ripening, but details of the potential roles and the relationship between these two regulators in the synthesis of volatiles are lacking. Here, we show substantial reductions in fatty acid and carotenoid-derived volatiles in tomato slnor and sldml2 mutants. An unexpected finding is the redundancy and divergence in volatile profiles, biosynthetic gene expression, and DNA methylation in slnor and sldml2 mutants relative to wild-type tomato fruit. Reduced transcript levels are accompanied by hypermethylation of promoters, including the NAC-NOR target gene lipoxygenase (SlLOXC) that is involved in fatty acid-derived volatile synthesis. Interestingly, NAC-NOR activates SlDML2 expression by directly binding to its promoter both in vitro and in vivo. Meanwhile, reduced NAC-NOR expression in the sldml2 mutant is accompanied by hypermethylation of its promoter. These results reveal a relationship between SlDML2-mediated DNA demethylation and NAC-NOR during tomato fruit ripening. In addition to providing new insights into the metabolic modulation of flavor volatiles, the outcome of our study contributes to understanding the genetics and control of fruit ripening and quality attributes in tomato.
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http://dx.doi.org/10.1111/nph.18301DOI Listing
September 2022

Detection of ovarian cancer using plasma cell-free DNA methylomes.

Clin Epigenetics 2022 06 9;14(1):74. Epub 2022 Jun 9.

Sun Yat-Sen Memorial Hospital of Sun Yat-sen University, Guangzhou, China.

Background: Ovarian cancer (OC) is a highly lethal gynecologic cancer, and it is hard to diagnose at an early stage. Clinically, there are no ovarian cancer-specific markers for early detection. Here, we demonstrate the use of cell-free DNA (cfDNA) methylomes to detect ovarian cancer, especially the early-stage OC.

Experimental Design: Plasma from 74 epithelial ovarian cancer patients, 86 healthy volunteers, and 20 patients with benign pelvic masses was collected. The cfDNA methylomes of these samples were generated by cell-free methylated DNA immunoprecipitation and high-throughput sequencing (cfMeDIP-seq). The differentially methylated regions (DMRs) were identified by the contrasts between tumor and non-tumor groups, and the discrimination performance was evaluated with the iterative training and testing method.

Results: The DMRs identified for cfDNA methylomes can well discriminate tumor groups and non-tumor groups (ROC values from 0.86 to 0.98). The late-stage top 300 DMRs are more late-stage-specific and failed to detect early-stage OC. However, the early-stage markers have the potential to discriminate all-stage OCs from non-tumor samples.

Conclusions: This study demonstrates that cfDNA methylomes generated with cfMeDIP-seq could be used to identify OC-specific biomarkers for OC, especially early OC detection. To detect early-stage OC, the biomarkers should be directly identified from early OC plasma samples rather than mix-stage ones. Further exploration of DMRs from a k larger early-stage OC cohort is warranted.
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http://dx.doi.org/10.1186/s13148-022-01285-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185905PMC
June 2022

Feature-preserving simplification framework for 3D point cloud.

Sci Rep 2022 Jun 8;12(1):9450. Epub 2022 Jun 8.

School of Information Science and Technology, Northwest University, Xi'an, 710127, Shaanxi, China.

To obtain a higher simplification rate while retaining geometric features, a simplification framework for the point cloud is proposed. Firstly, multi-angle images of the original point cloud are obtained with a virtual camera. Then, feature lines of each image are extracted by deep neural network. Furthermore, according to the proposed mapping relationship between the acquired 2D feature lines and original point cloud, feature points of the point cloud are extracted automatically. Finally, the simplified point cloud is obtained by fusing feature points and simplified non-feature points. The proposed simplification method is applied to four data sets and compared with the other six algorithms. The experimental results demonstrate that our proposed simplification method has the superiority in terms of both retaining geometric features and high simplification rate.
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http://dx.doi.org/10.1038/s41598-022-13550-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177861PMC
June 2022

A Baseline Study of Oxygen Saturation in Parafoveal Vessels Using Visible Light Optical Coherence Tomography.

Front Med (Lausanne) 2022 12;9:886576. Epub 2022 May 12.

Department of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, MD, United States.

The retinal macula is at the center of our visual field, and thus pathological damage in the macula significantly impacts an individual's quality of life. The parafoveal vessels form the inner retina provide oxygen perfusion, and the measurement of parafoveal oxygen saturation (sO) can evaluate macular metabolism and provide pathophysiological insight. In this paper, for the first time, we present a baseline study of microvascular oxygen saturation (sO) in perifoveal macular region using visible light optical coherence tomography (VIS-OCT) on normal eyes. The arterial and venous sO from all eyes was 92.1 ± 7.1 (vol %) and 48.4 ± 5.0 (vol %) (mean ± SD), respectively. Arteriovenous sO difference was 43.8 ± 9.5 (vol %). Marginal correlation was found between venous sO and intraocular pressure (IOP) among eyes. No significant correlation was found between sO and vessel topological features, including length, diameter, and distance to fovea. This baseline study could serve as a benchmark for the future sO investigation of retinal macular pathologies.
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http://dx.doi.org/10.3389/fmed.2022.886576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133487PMC
May 2022

Exosomal Circsafb2 Reshaping Tumor Environment to Promote Renal Cell Carcinoma Progression by Mediating M2 Macrophage Polarization.

Front Oncol 2022 12;12:808888. Epub 2022 May 12.

Department of Nephrology, The First Hospital of China Medical University, Shenyang, China.

Background: Macrophages are the most abundant infiltrating immune-related stromal cells present in and around tumors, showing different phenotypes and functions. M2 macrophages mainly exert immunosuppressive functions and promote tumor growth. Exosomes are emerging as important mediators of cross-talk between tumor cells and the microenvironment. CircRNAs are novel members of non-coding RNAs that regulate cancer proliferation and progression. However, the mechanism by which exosomal circRNA regulates macrophage polarization in renal cell carcinoma (RCC) is still largely unknown.

Methods: RCC-derived exosomes were characterized using transmission electron microscopy and nanoparticle tracking analysis (NTA). CCK-8, wound healing, and Transwell assays were performed to assess whether exosomes would affect the proliferation, migration, and invasion of RCC. Furthermore, we performed a bioinformatics analysis to identify circRNAs in RCC serum-derived exosomes from the GEO database. The fluorescence hybridization (FISH) assay was used to detect the cellular distribution of circSAFB2. Bioinformatics analyses (StarBase 2.0) were used to pool the miRNA targets of circSAFB2. Luciferase assays were performed to verify the direct interactions. Western blotting was used to detect markers of macrophage M2 polarization. Lastly, mouse xenograft and bioluminescence imaging were used to examine the clinical relevance of exosomal circSAFB2 .

Results: We report the circRNA derived from SAFB2 and evaluate its biological function in promoting the immune escape of RCC. We found that circSAFB2 was highly expressed in RCC tissues and RCC-derived exosomes. Furthermore, we demonstrated that exosomal circSAFB2 mediates the polarization of M2 macrophages through the miR-620/JAK1/STAT3 axis to promote RCC metastasis.

Conclusions: Our data first demonstrated that circSAFB2 leads to immune escape from RCC by mediating M2 macrophage polarization the miR-620/JAK1/STAT3 axis. These findings indicate a novel molecular mechanism of exosomal circSAFB2 in the progression of RCC and implicate circSAFB2 as a target for exosome-mediated tumor immune evasion.
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http://dx.doi.org/10.3389/fonc.2022.808888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133324PMC
May 2022

Early hematopoietic injury triggered by benzene characterized with inhibition of erythrocyte differentiation involving the mollicutes_RF39-derived citrulline.

Chemosphere 2022 Sep 18;303(Pt 1):135009. Epub 2022 May 18.

Department of Occupational Health and Environmental Health, School of Public Health, Capital Medical University, Beijing, 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, 100069, China. Electronic address:

Benzene poisoning is a common adverse blood outcome in occupational workers, manifested by hematopoietic dysfunction. However, the specific phenotype and its mechanisms of early hematopoietic toxicity caused by benzene remain unclear. After 15 days of exposure, the WBC levels were not significantly altered in benzene-exposed mice. However, the level of red blood cells (RBC) showed a significant decrease, and it was significantly and negatively correlated with urinary S-phenylmercapturic acid (SPMA). Notably, 5 mg/kg benzene exposure significantly inhibited the renewal capacity and the number of colony formation of hematopoietic stem progenitor cells in mice, especially erythrocyte differentiation. These results suggested that the early hematopoietic toxicity phenotype caused by benzene was dominated by inhibition of erythroid differentiation rather than WBC-related inflammation. To further understand the underlying mechanisms of benzene-induced early hematopoietic toxicity, 16 S rRNA sequencing and plasma metabolites analysis were conducted to investigate the impact of benzene exposure for 15 days on microbial composition and metabolic profile of mice. We found that short-term benzene exposure induced disturbances in gut microbiota and metabolism. The relative abundance of Mollicutes_RF39 at order levels was significantly reduced in benzene-exposed mice and was strongly correlated with hematopoietic indicators and urinary benzene markers. Interestingly, Mollicutes_RF39 might disturb the levels of eight metabolites, whereas Citrulline was highly linked to Mollicutes_RF39 (r = 0.862, P = 0.000). Consequently, Mollicutes_RF39-derived Citrulline might be the key regulator of early hematopoietic injury induced by benzene exposure. These findings promote the understanding of early hematotoxicity phenotypes and provide new perspectives on the underlying mechanisms of benzene-induced hematotoxicity.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135009DOI Listing
September 2022

Vitamin A supplementation ameliorates prenatal valproic acid-induced autism-like behaviors in rats.

Neurotoxicology 2022 07 17;91:155-165. Epub 2022 May 17.

Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, 123 Tianfei Alley, Mochou Road, Nanjing 210004, PR China; Institute of Pediatrics, Nanjing Medical University, 72 Guangzhou Road, Nanjing 210008, PR China. Electronic address:

Introduction: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social deficits and repetitive stereotyped behaviors. Prenatal exposure to the anticonvulsant drug valproic acid (VPA) is reported to induce ASD in human and ASD-like phenotypes in rodents. Unfortunately, the etiology and pathogenesis of ASD remains unclear.

Methods: Pregnant rats received an intraperitoneal injection of 600 mg/kg VPA on E12.5 to construct the ASD rat model in offspring. The different expression of long non-coding RNA (lncRNA) and mRNA profiles in the hippocampus were determined by RNA sequencing to investigate potential mechanisms of VPA-induced ASD. Gene Ontology (GO) and pathway enrichment analysis were performed to predict the function of dysregulated lncRNAs. Co-expression network and real-time polymerase chain reaction (RT-PCR) analysis were conducted to validate the potential regulatory lncRNA-mRNA network.

Results: VPA increased the total distance, time spent in the central zone and self-grooming (open field test) in rats. Meanwhile, VPA induced social impairment (three-chamber sociability test) and repetitive behaviors (marble burying test). A total of 238 lncRNAs and 354 mRNAs were differentially expressed in the VPA group. In addition, the dysregulated lncRNAs were involved in neural function and developmental processes of ASD. 5 lncRNAs and 7 mRNAs were differently expressed and included in the lncRNA-mRNA co-expression network. RT-PCR confirmed the upregulation of 4 lncRNAs and 6 mRNAs, and identified a potential regulatory network of NONRATT021475.2 (lncRNA) and Desert hedgehog (Dhh). Moreover, VPA decreased the serum vitamin A (VA) levels in offspring rats on postnatal day (PND) 21 and 49. Importantly, VA supplementation significantly restored VPA-induced autism-related behaviors and upregulation of NONRATT021475.2 and Dhh in the hippocampus of ASD rats.

Conclusion: This study not only contributed to understand the importance of lncRNAs and mRNAs in the progression of ASD, but also identified VA as a potential therapy for the condition.

Data Availability: The data that support the findings of this study are available from the corresponding author with reasonable request.
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http://dx.doi.org/10.1016/j.neuro.2022.05.008DOI Listing
July 2022
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