Publications by authors named "Jingyi Shen"

34 Publications

Assessment of Prophylactic Carbapenem Antibiotics Administration for Severe Acute Pancreatitis: An Updated Systematic Review and Meta-Analysis.

Digestion 2022 Jan 13:1-9. Epub 2022 Jan 13.

The Emergency Department, Zhuji People's Hospital, Shaoxing, China.

Background: The effectiveness of prophylactic antibiotics in severe acute pancreatitis (SAP) remains a debatable issue. This meta-analysis aimed to determine the efficacy of prophylactic carbapenem antibiotics in SAP.

Methods: This meta-analysis of prophylactic carbapenem antibiotics for SAP was conducted in PubMed, EMBASE, Web of Science, MEDLINE, and Cochrane Library up to February 2021. The related bibliographies were manually searched. The primary outcomes involved infected pancreatic or peripancreatic necrosis, mortality, complications, infections, and organ failure.

Results: Seven articles comprised 5 randomized controlled trials and 2 retrospective observational studies, including 3,864 SAP participants. Prophylactic carbapenem antibiotics in SAP were associated with a statistically significant reduction in the incidence of infections (odds ratio [OR]: 0.27; p = 0.03) and complications (OR: 0.48; p = 0.009). Nevertheless, no statistically significant difference was demonstrated in the incidence of infected pancreatic or peripancreatic necrosis (OR: 0.74; p = 0.24), mortality (OR: 0.69; p = 0.17), extrapancreatic infection (OR: 0.64, p = 0.54), pulmonary infection (OR: 1.23; p = 0.69), blood infection (OR: 0.60; p = 0.35), urinary tract infection (OR: 0.97; p = 0.97), pancreatic pseudocyst (OR: 0.59; p = 0.28), fluid collection (OR: 0.91; p = 0.76), organ failure (OR: 0.63; p = 0.19), acute respiratory distress syndrome (OR: 0.80; p = 0.61), surgical intervention (OR: 0.97; p = 0.93), dialysis (OR: 2.34; p = 0.57), use of respirator or ventilator (OR: 1.90; p = 0.40), intensive care unit treatment (OR: 2.97; p = 0.18), and additional antibiotics (OR: 0.59; p = 0.28) between the experimental and control groups.

Conclusions: It is not recommended to administer routine prophylactic carbapenem antibiotics in SAP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000520892DOI Listing
January 2022

Inhibition of IL-1 Receptor-Associated Kinase 1 Decreases Murine Acute Graft-versus-Host Disease While Preserving the Graft-versus-Lymphoma Effect.

Transplant Cell Ther 2021 Dec 9. Epub 2021 Dec 9.

Blood Diseases Institute, Xuzhou Medical University, Xuzhou, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, China. Electronic address:

Activation of antigen-presenting cells (APCs) is crucial in initiating inflammation and alloreaction during acute graft-versus-host disease (aGVHD), a common life-threatening complication of allogeneic hematopoietic cell transplantation (allo-HCT). IL-1 receptor-associated kinase 1 (IRAK1) regulates the activation of APCs in inflammatory settings, and inhibition of IRAK1 might decrease APC activation and aGVHD. This study was conducted to explore the impact of IRAK1 inhibition on APC activation and aGVHD in mice. We administered a selective IRAK1 inhibitor, Jh-X-119-01, to recipient mice undergoing allo-HCT or co-challenged by A20 lymphoma cells. We assessed aGVHD and the graft-versus-lymphoma (GVL) effect. T cell and APC activations were analyzed as well. Jh-X-119-01 was associated with increased survival and decreased aGVHD of recipients. Jh-X-119-01 decreased the proportions of Th1 cells and Tc1 cells in the aGVHD model and in the in vitro mixed lymphocyte reaction. The IRAK1 inhibitor reduced production of TNFα and IFNγ in macrophages of recipient mice. In in vitro cultured bone marrow dendritic cells (BMDCs), Jh-X-119-01 decreased productions of inflammatory cytokines, reduced expression levels of CD80 and CD86, and decreased protein levels of antiapoptotic Bcl2 and phosphorylated NF-κB p65. RNA-seq analysis showed that Jh-X-119-01 had an impact on several pathophysiologic processes of BMDCs, including reduction of GVHD-related genes and regulation of helper T cell differentiation. Importantly, IRAK1 inhibition did not impair cytotoxic function of T cells or the allo-HCT-related GVL effect against A20 lymphoma cells. In addition, the IRAK1 inhibitor did not retard recovery of hematopoietic cells in blood or bone marrow. Our findings show that selective IRAK1 inhibition ameliorates murine aGVHD but preserves the GVL effect. Our findings may have implications for the use of an IRAK1 inhibitor in allo-HCT.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jtct.2021.12.001DOI Listing
December 2021

Architectural Glazed Tiles Used in Ancient Chinese Screen Walls (15th-18th Century AD): Ceramic Technology, Decay Process and Conservation.

Materials (Basel) 2021 Nov 24;14(23). Epub 2021 Nov 24.

School of Civil Engineering, Shandong University, Jinan 250012, China.

The glazed tile is an important building material used throughout the history of traditional Chinese architecture. Architectural glazed tiles used to decorate the screen walls of ancient China are studied scientifically for the first time. More than 30 glazed tile samples from the screen walls of the 15th to 18th century AD of the Hancheng Confucian Temple and Town God's Temple in Shaanxi Province were carefully investigated using SEM-EDS and XRD. Microstructure and chemistry indicated the raw materials, the recipes and the technological choices used to produce the paste and glaze of the glazed tile samples studied. The causes for the key degradation processes of these glazed tiles used as building materials in the screen walls have also been discussed. This work has clear implications for the restoration and conservation treatments on these kinds of ancient Chinese building materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14237146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658199PMC
November 2021

Effect of artificial intelligence-aided colonoscopy for adenoma and polyp detection: a meta-analysis of randomized clinical trials.

Int J Colorectal Dis 2021 Nov 11. Epub 2021 Nov 11.

Department of Gastroenterology, HwaMei Hospital, University of Chinese Academy of Sciences, Haishu District, Northwest Street 41, Ningbo, 315010, Zhejiang, China.

Background: This meta-analysis aimed to determine whether artificial intelligence (AI) improves colonoscopy outcome metrics i.e. adenoma detection rate (ADR) and polyp detection rate (PDR).

Methods: Two authors independently searched Web of Science, PubMed, Science Direct, and Cochrane Library to find all published research before July 2021 that has compared AI-aided colonoscopy with routine colonoscopy (RC) for detection of adenoma and polyp.

Results: This meta-analysis included 10 RCTs with 6629 individuals in AI-aided (n = 3300) and routine (n = 3329) groups. The results showed that both ADR (RR, 1.43; P < 0.001) and PDR (RR, 1.44; P < 0.001) using AI-aided endoscopy were significantly greater when compared with RC. The adenomas detected per colonoscopy (APC) (WMD, 0.25; P = 0.009), polyps detected per colonoscopy (PPC) (WMD, 0.52; P < 0.001), and sessile serrated lesions detected per colonoscopy (SSLPC) (RR, 1.53; P < 0.001) were significantly higher in the AI-aided group compared with the RC group. Subgroup analysis based on size, location, and shape of adenomas and polyps demonstrated that, except for in the cecum and pedunculated adenomas or polyps, the AI-aided groups of the other subgroups are more advantageous. Withdrawal time was longer in the AI-aided group when biopsies were included, while withdrawal time excluding biopsy time showed no significant difference.

Conclusions: AI-aided polyp detection system significantly increases lesion detection rate. In addition, lesion detection by AI is hardly affected by factors such as size, location, and shape.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00384-021-04062-xDOI Listing
November 2021

Cr release after Cr(III) and Cr(VI) enrichment from different layers of cast iron corrosion scales in drinking water distribution systems: the impact of pH, temperature, sulfate, and chloride.

Environ Sci Pollut Res Int 2021 Oct 26. Epub 2021 Oct 26.

Department of Environmental Engineering, School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.

Chromium accumulated from source water and pipeline lining materials in corrosion scales could potentially be released into bulk water in drinking water distribution systems (DWDS). This study examined the influence of pH (pH 4, pH 5.5, pH 7, pH 8.5, pH 10), temperature (5 °C, 15 °C, 25 °C), sulfate (50 mg/L, 150 mg/L, 250 mg/L), and chloride (50 mg/L, 150 mg/L, 250 mg/L) on chromium accumulation and release between iron corrosion scale phase and the surrounding water phase. For the first time, the accumulation and release behaviors of chromium were assessed and compared in two distinct layers of iron corrosion scales based on the speciation distributions of heavy metals. Results showed that in the outer and inner layers of corrosion scales, chromium exhibited an almost similar trend but significant differences in quantity, with the outer layer accumulating less and releasing more. In particular, the average difference of chromium released after Cr(VI) enrichment from the outer and inner layers was 50.53 μg/L under the same conditions. Further studies conclusively showed that in Cr(VI) accumulation process, a portion of Cr(VI) would be reduced to Cr(III) by Fe(II) in iron corrosion scales. The mechanisms of chromium retention based on different iron (oxyhydr)oxides were discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15754-wDOI Listing
October 2021

Comparing Hypofractionated With Conventional Fractionated Radiotherapy After Breast-Conserving Surgery for Early Breast Cancer: A Meta-Analysis of Randomized Controlled Trials.

Front Oncol 2021 1;11:753209. Epub 2021 Oct 1.

Department of General Surgery, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China.

Background: The purpose of this meta-analysis was to compare the safety and efficacy between hypofractionated and conventional fractionation radiotherapy in patients with early-stage breast cancer after breast-conserving surgery.

Methods: We conducted a comprehensive search of PubMed, Embase, Web of Science, and the Cochrane Library to identify relevant randomized controlled trials (RCTs) published before February 2021. At the same time, the hazard ratio (HR), risk ratio (RR), and 95% confidence interval (CI) were calculated to evaluate local recurrence (LR), relapse-free survival (RFS), overall survival (OS), adverse events, and cosmetic outcomes.

Results: A total of 14 articles were included in this meta-analysis. Four thousand eight hundred and sixty-nine patients were randomly assigned to the control group to receive conventional radiotherapy (CFRT); 6,072 patients were randomly assigned to the experimental group and treated with hypofractionated radiotherapy (HFRT). The results showed that there was no statistical difference between HFRT and CFRT in LR (HR = 0.99, 95%CI = 0.97-1.02,  = 0.476), RFS (HR = 0.99, 95%CI = 0.97-1.02,  = 0.485), OS (HR = 1.00, 95%CI = 0.97-1.03,  = 0.879), and cosmetic outcomes (RR = 1.03, 95%CI = 0.95-1.12,  = 0.53). In addition, HFRT showed fewer severe adverse reactions such as acute skin toxicity, induration, breast atrophy, and pain.

Conclusion: Our results suggest that there is no statistical difference between HFRT and CFRT in terms of LR, RFS, OS, and cosmetic outcomes. HFRT reduces the risk of developing toxicity reactions compared to CFRT. HFRT may be a better option for patients with early breast cancer after breast-conserving surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.753209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8518530PMC
October 2021

Thymus Degeneration and Regeneration.

Front Immunol 2021 1;12:706244. Epub 2021 Sep 1.

Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University, Xuzhou, China.

The immune system's ability to resist the invasion of foreign pathogens and the tolerance to self-antigens are primarily centered on the efficient functions of the various subsets of T lymphocytes. As the primary organ of thymopoiesis, the thymus performs a crucial role in generating a self-tolerant but diverse repertoire of T cell receptors and peripheral T cell pool, with the capacity to recognize a wide variety of antigens and for the surveillance of malignancies. However, cells in the thymus are fragile and sensitive to changes in the external environment and acute insults such as infections, chemo- and radiation-therapy, resulting in thymic injury and degeneration. Though the thymus has the capacity to self-regenerate, it is often insufficient to reconstitute an intact thymic function. Thymic dysfunction leads to an increased risk of opportunistic infections, tumor relapse, autoimmunity, and adverse clinical outcome. Thus, exploiting the mechanism of thymic regeneration would provide new therapeutic options for these settings. This review summarizes the thymus's development, factors causing thymic injury, and the strategies for improving thymus regeneration.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.706244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442952PMC
December 2021

Patterns and predictors of adolescent life change during the COVID-19 pandemic: a person-centered approach.

Curr Psychol 2021 Sep 13:1-15. Epub 2021 Sep 13.

Institute of Developmental Psychology, Beijing Normal University, No. 19 Xin Jie Kou Wai Street, Hai Dian District, Beijing, 100875 China.

The present study investigated patterns of adolescent life changes across multiple life domains and utilized a holistic-interactionistic perspective to examine their individual, familial, and societal correlates with a sample of 2544 Chinese parent-adolescent dyads. Adolescents were aged from 10 to 19 years old (50.16% girls). Latent profile analysis revealed five life change profiles, including three improved profiles at various degrees, one unchanged profile, and one worsened profile. The majority of adolescents had an improved or unchanged life. Multinomial logistic regression analyses found that most of the individual, familial, and societal factors predicted the group memberships. Notably, parent-adolescent conflict was a significant factor that predicted memberships of all patterns. These findings show the resilience of adolescents and indicate the need for policies and interventions that consider the holistic nature of adolescents' person-context system, especially during a global crisis such as the COVID-19 pandemic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12144-021-02204-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435363PMC
September 2021

Exploration of spatial distribution of brain metastasis from small cell lung cancer and identification of metastatic risk level of brain regions: a multicenter, retrospective study.

Cancer Imaging 2021 Jun 13;21(1):41. Epub 2021 Jun 13.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, 88 Keling Road, Suzhou New District, Suzhou, 215163, Jiangsu, China.

Objectives: This study aimed to explore the spatial distribution of brain metastases (BMs) from small cell lung cancer (SCLC) a homogenous sample, and to identify the metastatic risk levels in brain regions.

Methods: T1-enhanced magnetic resonance imaging (MRI) from SCLC patients were retrospectively reviewed from three medical institutions in China. All images were registered to the standard brain template provided by the Montreal Neurological Institute (MNI) 152 database, followed by transformation of the location of all BMs to the space of standard brain. The MNI structural atlas and Anatomical Automatic Labeling (AAL) atlas were then used to identify the anatomical brain regions, and the observed and expected rates of BMs were compared using 2-tailed proportional hypothesis testing. The locations and sizes of brain lesions were analyzed after image standardization.

Results: A total of 215 eligible patients with 1033 lesions were screened by MRI, including 157 (73%) males and 58 (27%) females. The incidence of crucial structures were as follows: hippocampus 0.68%, parahippocampal 0.97%, brainstem 2.05%, cauate 0.68%, putamen 0.68%, pallidum 0.2%, thalamus 1.36%. No BMs were found in the amygdala, pituitary gland, or pineal gland. The cumulative frequency of the important structures was 6.62%. Based on the results of MNI structural atlas, the cerebellum, deep white matter and brainstem was identified as a higher risk region than expected for BMs (P = 9.80 ×10, 9.04 ×10), whereas temporal lobe were low-risk regions (P = 1.65 ×10). More detailed AAL atlas revealed that the low-risk regions for BMs was inferior frontal gyrus (P = 6.971 ×10), while the high-risk regions for BMs was cerebellar hemispheres (P = 1.177 ×10).

Conclusion: Many crucial structures including the hippocampus, parahippocampus, pituitary gland and thalamus etc. have low frequency of brain metastases in a population of SCLC patients. This study provides the help to investigate the clinical feasibility of HA-WBRT and non-uniform dose of PCI in a population of SCLC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40644-021-00410-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201893PMC
June 2021

Overexpression of macrophage-colony stimulating factor-1 receptor as a prognostic factor for survival in cancer: A systematic review and meta-analysis.

Medicine (Baltimore) 2021 Mar;100(12):e25218

Cancer Center, The People's Hospital of Guangxi Zhuang Autonomous Region, China.

Background: The relation between the expression of macrophage-colony stimulating factor-1 receptor (CSF-1R) and prognosis of cancer patients has been evaluated in multiple studies, but the results remain controversial. We, therefore, performed a meta-analysis and systematic review to figure out the role of CSF-1R in the prognosis of patients with cancer.

Methods: Several databases were searched, including Web of Science, PubMed, and EMBASE. All human studies were published as full text. The Newcastle-Ottawa risk of bias scale was applied to evaluate the research. We extracted hazard ratios (HRs) with 95% confidence interval (95% CI) which assessed progression-free survival (PFS) and overall survival (OS) in order to assess the impacts of CSF-1R on the prognosis of cancer patients.

Results: A total of 12 citations were identified, with studies including 2260 patients in different cancer types that met the eligibility criteria. It was suggested in a pooled analysis that the over-expression of CSF-1R was significantly related to worse PFS (HR: 1.68; P < .001, 1.25-2.10, 95% CI) and also poorer OS (HR=1.28; P < .001, 1.03-1.54, 95% CI). Analysis in subgroups indicated over-expressed CSF-1R was significantly associated with worse OS in hematological malignancy (HR = 2.29; P < .001, 1.49-3.09, 95% CI; model of fixed-effects; I2 = 0.0%, P < .001). Sensitivity analysis suggested that there was no study influencing the stability of the results.

Conclusions: The overexpression of CSF-1R was significantly predictive of worse prognosis in those who suffer from different kinds of malignancies, particularly in hematological malignancy, which indicates that it might be a potential biomarker of prognosis in cancer survival and a potential molecular target in the treatment of malignant tumors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025218DOI Listing
March 2021

Perceived Social Support Protects Lonely People Against COVID-19 Anxiety: A Three-Wave Longitudinal Study in China.

Front Psychol 2020 6;11:566965. Epub 2020 Nov 6.

Faculty of Psychology, Beijing Normal University, Beijing, China.

The isolation necessary to prevent the spread of the coronavirus disease 2019 (COVID-19) can give rise to anxiety, especially for lonely people who often feel upset without others' company. Although isolated from others, people can still receive support from others, which might lower their COVID-19 anxiety. To examine the relationship between loneliness, perceived social support, and anxiety, we measured 222 Chinese participants' (54.50% female, = 31.53, = 8.17) trait loneliness, chronic anxiety before the outbreak, COVID-19 anxiety at the peak and decline stages of COVID-19, and their perceived social support across the three time points. The results showed that people's perceived social support dramatically increased from the pre-pandemic to the peak COVID-19 stage, and remained stable during the decline of COVID-19 stage. In contrast, COVID-19 anxiety decreased from the peak to the decline stage. Further, perceived social support consistently moderated the relationship between loneliness with both chronic anxiety and COVID-19 anxiety. The current study provides initial evidence that perceived social support provides protection for lonely people in daily life as well as during unexpected disasters, which will contribute to finding ways to alleviate lonely people's anxiety during this global health crisis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyg.2020.566965DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7677578PMC
November 2020

Short-term stock market price trend prediction using a comprehensive deep learning system.

J Big Data 2020 28;7(1):66. Epub 2020 Aug 28.

School of Information Technology, Carleton University, Ottawa, ON Canada.

In the era of big data, deep learning for predicting stock market prices and trends has become even more popular than before. We collected 2 years of data from Chinese stock market and proposed a comprehensive customization of feature engineering and deep learning-based model for predicting price trend of stock markets. The proposed solution is comprehensive as it includes pre-processing of the stock market dataset, utilization of multiple feature engineering techniques, combined with a customized deep learning based system for stock market price trend prediction. We conducted comprehensive evaluations on frequently used machine learning models and conclude that our proposed solution outperforms due to the comprehensive feature engineering that we built. The system achieves overall high accuracy for stock market trend prediction. With the detailed design and evaluation of prediction term lengths, feature engineering, and data pre-processing methods, this work contributes to the stock analysis research community both in the financial and technical domains.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40537-020-00333-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467129PMC
August 2020

Camphor wood, a potentially harmful museum storage material: an analytical study using instrumental methods.

Authors:
Jingyi Shen

Environ Sci Pollut Res Int 2021 Sep 2;28(34):46458-46468. Epub 2020 Jul 2.

Shanghai Museum Conservation Center, Shanghai Museum, Shanghai, 200231, China.

Camphor wood is welcomed by museums due to its insect-repelling effect but the smell indicates a potential risk to the collections. In order to judge the suitability of camphor wood as a museum storage material, typical camphor wood (Cinnamomum camphora) samples aged for different years were evaluated by conducting the Oddy test. Gas chromatography-mass spectrometry (GC-MS), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDS), and time of flight-secondary ion mass spectrometry (ToF-SIMS) were applied to identifying the volatile organic compounds (VOCs) emitted by the materials and the corrosion products, respectively. The results showed that the camphor wood samples led to visible corrosion on copper and lead coupons. GC-MS indicated that the major VOCs emitted were terpenes and their derivatives, while XRD, EDS, and ToF-SIMS provided various clues to the corrosion mechanisms. Pb(CO)(OH)O and CuO were regarded as the major corrosion products of lead and copper coupons, respectively. The study provides the museum curators and the conservators with abundant information to reassess the application of camphor wood to museums as well as a different way to understand the mechanism of metallic corrosion caused by camphor wood.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-020-09446-0DOI Listing
September 2021

Four Loci Are Associated with Cardiorespiratory Fitness and Endurance Performance in Young Chinese Females.

Sci Rep 2020 06 22;10(1):10117. Epub 2020 Jun 22.

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Collaborative Innovation Center for Brain Science, Shanghai Jiao Tong University, Shanghai, 200240, China.

Cardiorespiratory fitness (CRF) and endurance performance are characterized by a complex genetic trait with high heritability. Although research has identified many physiological and environmental correlates with CRF, the genetic architecture contributing to CRF remains unclear, especially in non-athlete population. A total of 762 Chinese young female participants were recruited and an endurance run test was used to determine CRF. We used a fixed model of genome-wide association studies (GWAS) for CRF. Genotyping was performed using the Affymetrix Axiom and illumina 1 M arrays. After quality control and imputation, a linear regression-based association analysis was conducted using a total of 5,149,327 variants. Four loci associated with CRF were identified to reach genome-wide significance (P < 5.0 × 10), which located in 15q21.3 (rs17240160, P = 1.73 × 10, GCOM1), 3q25.31 (rs819865, P = 8.56 × 10, GMPS), 21q22.3 (rs117828698, P = 9.59 × 10, COL18A1), and 17q24.2 (rs79806428, P = 3.85 × 10, PRKCA). These loci (GCOM1, GMPS, COL18A1 and PRKCA) associated with cardiorespiratory fitness and endurance performance in Chinese non-athlete young females. Our results suggest that these gene polymorphisms provide further genetic evidence for the polygenetic nature of cardiorespiratory endurance and be used as genetic biomarkers for future research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-67045-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723046PMC
June 2020

Association of Twice-Daily Radiotherapy With Subsequent Brain Metastases in Adults With Small Cell Lung Cancer.

JAMA Netw Open 2019 05 3;2(5):e190103. Epub 2019 May 3.

Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong, China.

Importance: Although thoracic twice-daily radiotherapy (TDRT) is one of the standards of care for small cell lung cancer, its association with brain metastases remains unknown.

Objective: To investigate the association of TDRT vs once-daily radiotherapy (ODRT) with brain metastases after prophylactic cranial irradiation in patients with small cell lung cancer.

Design, Setting, And Participants: In this multicenter cohort study, data on 778 consecutive patients with small cell lung cancer who had undergone thoracic radiotherapy (609 received ODRT and 169 received TDRT), chemotherapy, and prophylactic cranial irradiation were retrieved from the databases of 8 hospitals in China between July 1, 2003, and June 30, 2016. A 1:1 propensity score matching approach was used to control for confounding between the ODRT and TDRT groups. Confounding covariates included 8 demographic variables and 8 treatment-related covariates. Data analysis was conducted from November 1, 2017, to May 31, 2018, and reanalyzed for revision.

Exposures: The ODRT group received 50 to 66 Gy given in 25 to 33 fractions. The TDRT group received 45 Gy given in 30 fractions.

Main Outcomes And Measures: The primary end point was brain metastases. Secondary end points included progression-free survival and overall survival.

Results: Of the 778 patients (median age, 55 years [interquartile range, 48-61 years]), 204 were women and 574 were men. At a median follow-up of 23.6 months (interquartile range, 14.2-38.2 months), 131 patients (16.8%) experienced brain metastases. The rate of brain metastasis at 3 years in the TDRT group was significantly higher than in the ODRT group (26.0% vs 16.9%; hazard ratio, 1.55; 95% CI, 1.06-2.26; P = .03). Of the 338 matched patients (169 in the ODRT group vs 169 in the TDRT group), 60 (17.8%) experienced brain metastases, with a rate at 3 years of 14.9% in the ODRT group vs 26.0% in the TDRT group (hazard ratio, 1.71; 95% CI, 1.02-2.88; P = .04). Progression-free survival was similar in both the whole cohort and the matched cohort. Median overall survival in the ODRT group tended to be significantly longer than in the TDRT group after matching (47.2 vs 32.8 months; hazard ratio, 1.41; 95% CI, 0.99-2.01; P = .06).

Conclusions And Relevance: In this study, patients with small cell lung cancer who received thoracic TDRT appeared to have a higher risk of brain metastases than those who received ODRT, which supports the need for further prospective randomized clinical trials, especially in China and other parts of Asia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1001/jamanetworkopen.2019.0103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6537825PMC
May 2019

Proteasome β5 subunit overexpression improves proteostasis during aging and extends lifespan in Drosophila melanogaster.

Sci Rep 2019 02 28;9(1):3170. Epub 2019 Feb 28.

Department of Biology, Harvey Mudd College, Claremont, CA, 91711, USA.

The β5 subunit of the proteasome has been shown in worms and in human cell lines to be regulatory. In these models, β5 overexpression results in upregulation of the entire proteasome complex which is sufficient to increase proteotoxic stress resistance, improve metabolic parameters, and increase longevity. However, fundamental questions remain unanswered, including the temporal requirements for β5 overexpression and whether β5 overexpression can extend lifespan in other species. To determine if adult-only overexpression of the β5 subunit can increase proteasome activity in a different model, we characterized phenotypes associated with β5 overexpression in Drosophila melanogaster adults. We find that adult-only overexpression of the β5 subunit does not result in transcriptional upregulation of the other subunits of the proteasome as they do in nematodes and human cell culture. Despite this lack of a regulatory role, boosting β5 expression increases the chymotrypsin-like activity associated with the proteasome, reduces both the size and number of ubiquitinated protein aggregates in aged flies, and increases longevity. Surprisingly, these phenotypes were not associated with increased resistance to acute proteotoxic insults or improved metabolic parameters.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-39508-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6395709PMC
February 2019

Evaluating the suitability of museum storage or display materials for the conservation of metal objects: a study on the conformance between the deposited metal film method and the Oddy test.

Environ Sci Pollut Res Int 2018 Dec 16;25(35):35109-35129. Epub 2018 Oct 16.

Conservation Center, Shanghai Museum, 1118 Longwu Road, Shanghai, 200231, China.

Storing and exhibiting cultural heritages are the basic social functions of museum. Since it is impossible to store or display objects without using containers, cases, or holders, the equipment quality is of great importance. Evaluating the suitability of the equipment should not only learn the mechanical behavior but also focus on the materials because some of them (such as woods) may do harm to the objects due to contaminants released. A convenient test by the deposited metal film method has been proposed previously in order to evaluate more potential museum materials in limited time. The conformance between this method and the Oddy test, the classic method for evaluating and selecting museum materials, is mainly studied in this work. The two testing systems were compared from several aspects such as sample appearance, corrosion product, surface morphology, and metallic content by naked eye and modern characterization measures like X-ray diffraction, scanning electron microscopy, and energy-dispersive spectroscopy. The corrosion mechanisms were deduced according to the corrosion products, including Cu → CuO → CuO and Cu → CuO → Cu(OH)·HO → Cu(HCOO)(OH). The suitability of potential materials for the conservation of metal objects was defined according to the metallic contents of coupons (calculated by atomic ratio) which were classified by the Oddy test. The critical values distinguishing permanently usable from temporarily usable are approximately determined as 70% for copper and 75% for silver, and those distinguishing temporarily usable from unusable are approximately determined as 55% for copper and 60% for silver. The corresponding metal films were classified based on the metallic content standard derived, and then typical appearances of the films assigned to different suitability levels were suggested. Special phenomena, such as the failure in detecting some corrosion products, is attributed to low yield and uneven distribution of ultrafine corrosion products on the films, the covering effect caused by other corrosion products on the copper coupons, and the weakening effect resulted from intensive metal peaks, while some unexpected corrosion conditions on the coupons and the films, are related to the characteristics of general corrosion and pitting corrosion. The results indicate the potential application of the deposited metal film method, giving an optional choice to evaluate and select museum materials with less time. The evaluation methods were preliminarily established from three aspects, such as artificial judgment, metallic content analysis, and corrosion product identification. The artificial judgment is generally applicable, and the other two are useful for verifying the result if possible. More actual cases and further calibration work are essential for further development of the deposited metal film method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-018-3436-6DOI Listing
December 2018

Association of fat mass and obesity-associated and retinitis pigmentosa guanosine triphosphatase (GTPase) regulator-interacting protein-1 like polymorphisms with body mass index in Chinese women.

Endocr J 2018 Jul 14;65(7):783-791. Epub 2018 Apr 14.

Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), the Collaborative Innovation Center for Brain Science, Shanghai Jiao Tong University, Shanghai 200030, China.

Body mass index (BMI) is the most commonly used quantitative measure of adiposity. It is a kind of complex genetic diseases which are caused by multiple susceptibility genes. The first intron of fat mass and obesity-associated (FTO) has been widely discovered to be associated with BMI. Retinitis pigmentosa GTPase regulator-interacting protein-1 like (RPGRIP1L) is located in the upstream region of FTO and has been proved to be linked with obesity through functional tests. We carried out a genetic association analysis to figure out the role of the FTO gene and the RPGRIP1L gene in BMI. A quantitative traits study with 6,102 Chinese female samples, adjusted for age, was performed during our project. Among the twelve SNPs, rs1421085, rs1558902, rs17817449, rs8050136, rs9939609, rs7202296, rs56137030, rs9930506 and rs12149832 in the FTO gene were significantly associated with BMI after Bonferroni correction. Meanwhile, rs9934800 in the RPGRIP1L gene showed significance with BMI before Bonferroni correction, but this association was eliminated after Bonferroni correction. Our results suggested that genetic variants in the FTO gene were strongly associated with BMI in Chinese women, which may serve as targets of pharmaceutical research and development concerning BMI. Meanwhile, we didn't found the significant association between RPGRIP1L and BMI in Chinese women.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1507/endocrj.EJ17-0554DOI Listing
July 2018

Common variants in ZMIZ1 and near NGF confer risk for primary dysmenorrhoea.

Nat Commun 2017 04 27;8:14900. Epub 2017 Apr 27.

The Affiliated Hospital of Qingdao University &The Biomedical Sciences Institute of Qingdao University (Qingdao Branch of SJTU Bio-X Institutes), Qingdao University, Qingdao 266003, China.

Primary dysmenorrhoea, defined as painful menstrual cramps in the absence of pelvic pathology, is a common problem in women of reproductive age. Its aetiology and pathophysiology remain largely unknown. Here we performed a two-stage genome-wide association study and subsequent replication study to identify genetic factors associated with primary dysmenorrhoea in a total of 6,770 Chinese individuals. Our analysis provided evidence of a significant (P<5 × 10) association at rs76518691 in the gene ZMIZ1 and at rs7523831 near NGF. ZMIZ1 has previously been associated with several autoimmune diseases, and NGF plays a key role in the generation of pain and hyperalgesia and has been associated with migraine. These findings provide future directions for research on susceptibility mechanisms for primary dysmenorrhoea. Furthermore, our genetic architecture analysis provides molecular support for the heritability and polygenic nature of this condition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/ncomms14900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5414039PMC
April 2017

Turning Redundant Ligands into Treasure: A New Strategy for Constructing MIL-53(Al)@Nanoscale TiO2 Layers.

Chemistry 2015 Nov 14;21(48):17485-90. Epub 2015 Oct 14.

College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Siping Rd 1239, 200092 Shanghai (P.R. China).

A strategy for in situ fabrication of nanoscale-thin layers of anatase TiO2 coated on the metal-organic framework (MOF) material, MIL-53(Al), is developed. The preparation conditions for crystallized TiO2 are normally incompatible with the thermal and chemical stability of MOFs. Based on our strategy, we found that the redundant organic ligands (1,4-benzenedicarboxylic acid, H2 BDC) within the pores of the as-synthesized MOF play a key function in the protection and support of the framework during hydrothermal loading of the TiO2 precursor, as well as in preventing the infiltration of the precursor into the pores. After annealing, a nanoscale-thin layer of highly crystalline anatase TiO2 , with a thickness of 6-10 nm, was successfully attached to the external surface of the MIL-53(Al) crystals, while the porous framework remains intact. The core-shell structure of the [email protected] nanocomposite endows the resulting materials with additional optical response and enhanced moisture and chemical stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/chem.201503052DOI Listing
November 2015

The mitochondrial genome of an endangered native Singidia tilapia, Oreochromis esculentus: genome organization and control region polymorphism.

Mitochondrial DNA A DNA Mapp Seq Anal 2016 11 14;27(6):4344-4346. Epub 2015 Oct 14.

a Department of Aquaculture , College of Marine Sciences, Ningbo University , Ningbo , Zhejiang , People's Republic of China.

Singidia tilapia (Oreochromis esculentus) is a native Cichlid fish of important commercial value, distributed in Lake Victoria, East Africa. Due to its declining population levels in its natural habitat, this species has now been classified as a Critically Endangerd by the International Union for the Conservation of nature (IUCN). In the present study the complete nucleotide sequence of the mitochondrial genome (mtDNA) of O. esculentus was determined. In addition, polymorphism analysis based on the mtDNA's control region sequence was investigated on two of its remaining populations of Yala and Borabu as well as a phylogenetic consideration using 16S rRNA mtDNA genes to explore its position and relationship within Cichlidae fish. The length of the complete mitogenome of O. esculentus is 16 622 bp, containing the same order and an identical number of genes and regions with the other reported Cichlid fishes, which consists of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a putative non-coding region. The phylogenetic analysis shows O. esculentus being clustered within the Oreochromini sub-tribe of the Cichlidae. The high genetic diversity and low genetic differentiation between the two populations indicated the need for conservation of both the refuge ecosystems and the fish species under study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3109/19401736.2015.1089493DOI Listing
November 2016

Multifunctional mesoporous silica nanoparticles mediated co-delivery of paclitaxel and tetrandrine for overcoming multidrug resistance.

Int J Pharm 2015 Jul 5;489(1-2):318-30. Epub 2015 May 5.

Department of Pharmacy, Qilu Hospital of Shandong University, 107 Wenhua Xilu, Jinan 250012, PR China.

The objective of the study is to fabricate multifunctional mesoporous silica nanoparticles for achieving co-delivery of conventional antitumor drug paclitaxel (PTX) and the multidrug resistance reversal agent tetrandrine (TET) expecting to overcome multidrug resistance of MCF-7/ADR cells. The nanoparticles were facile to prepare by self-assemble in situ drug loading approach. Namely, PTX and TET were solubilized in the cetyltrimethylammonium bromide (CTAB) micelles and simultaneously silica resources hydrolyze and condense to form nanoparticles. The obtained nanoparticles, denoted as PTX/[email protected], exhibited pH-responsive release property with more easily released in the weak acidic environment. Studies on cellular uptake of nanoparticles demonstrated TET could markedly increase intracellular accumulation of nanoparticles. Furthermore, the PTX/[email protected] suppressed tumor cells growth more efficiently than only delivery of PTX ([email protected]) or the free PTX. Moreover, the nanoparticle loading drugs with a PTX/TET molar ratio of 4.4:1 completely reversed the resistance of MCF-7/ADR cells to PTX and the resistance reversion index was 72.3. Mechanism research showed that both TET and CTAB could arrest MCF-7/ADR cells at G1 phase; and besides PTX arrested cells at G2 phase. This nanocarrier might have important potential in clinical implications for co-delivery of multiple drugs to overcome MDR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpharm.2015.05.010DOI Listing
July 2015

In vitro and in vivo study of Gal-OS self-assembled nanoparticles for liver-targeting delivery of doxorubicin.

J Pharm Sci 2014 Mar 24;103(3):987-93. Epub 2014 Jan 24.

Department of Pharmaceutics, School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, People's Republic China.

A liver-targeting drug delivery system for doxorubicin (DOX), that is, DOX-loaded self-assembled nanoparticles based on galactosylated O-carboxymethyl chitosan-graft-stearic acid conjugates (Gal-OS/DOX), has been prepared. The objective of the present study was to investigate the preparation, in vitro release, in vivo pharmacokinetics, and tissue distribution of Gal-OS/DOX nanoparticles. The drug-loaded nanoparticles were spherical in shape with mean size of 181.9 nm. In vitro release profiles indicated that the release of DOX from Gal-OS/DOX nanoparticles behaved with a sustained and pH-dependent drug release. Pharmacokinetics study revealed Gal-OS/DOX nanoparticles exhibited a higher AUC value and a prolonged residence time of drug in the blood circulation than those of DOX solution. Furthermore, Gal-OS/DOX nanoparticles increased the uptake of DOX in liver and spleen, but decreased uptake in heart, lung, and kidney in the tissue distribution study. These results suggested that the Gal-OS/DOX nanoparticles could prolong blood circulation time, enhance the liver accumulation, and reduce the side effect especially the cardiotoxicity of DOX. In conclusion, Gal-OS/DOX nanoparticles could be a promising drug delivery system for liver cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jps.23875DOI Listing
March 2014

Self-assembled nanoparticles based on galactosylated O-carboxymethyl chitosan-graft-stearic acid conjugates for delivery of doxorubicin.

Int J Pharm 2013 Dec 17;458(1):31-8. Epub 2013 Oct 17.

Department of Pharmaceutics, School of Pharmaceutical Sciences, Shandong University, 44 Wenhua Xilu, Jinan 250012, PR China.

A novel polymer, i.e. galactosylated O-carboxymethyl chitosan-graft-stearic acid (Gal-OCMC-g-SA) was synthesized for liver targeting delivery of doxorubicin. The chemical structure was characterized by FT-IR, (1)H NMR and elemental analysis. Gal-OCMC-g-SA could self-assemble into nanoparticles with diameter of 160 nm by probe sonication in aqueous medium and exhibited a low critical aggregation concentration of 0.047 mg/mL. The DOX-loaded Gal-OCMC-g-SA (Gal-OCMC-g-SA/DOX) self-assembled nanoparticles were almost spherical in shape with an average diameter of less than 200 nm and zeta potential of around -10 mV. In vitro release revealed that the Gal-OCMC-g-SA/DOX nanoparticles exhibited a sustained and pH-dependent drug release manner. Furthermore, the hemolysis test demonstrated the good safety of Gal-OCMC-g-SA in blood-contacting applications. These results indicated that Gal-OCMC-g-SA/DOX nanoparticles were highly potential to be applied in cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpharm.2013.10.020DOI Listing
December 2013

Synthesis, characterization, in vitro and in vivo evaluation of PEGylated oridonin conjugates.

Int J Pharm 2013 Nov 21;456(1):80-6. Epub 2013 Aug 21.

Department of Pharmaceutics, College of Pharmacy, Shandong University, Jinan 250012, PR China.

Oridonin (ORI), a diterpenoid compound with promising antitumor activity, was proved to possess potent antileukemia efficacies in vitro and in vivo recently. However, the development and application of ORI was limited by its poor solubility and rapid plasma clearance. The purpose of this study was to solve these problems. PEGylated oridonin linked with succinic acid (SA) as spacer moiety (PEG-SA-ORI conjugate) was synthesized. mPEG amines with four specifications of molecular weight (MW) were utilized. All polymeric conjugates showed satisfactory aqueous solubility and in vitro studies implied that the drug solubility and release features of conjugates were relevant to PEGs. The drug solubility increased more when the MW of PEG was lower, while more significant sustained-release effect was shown with higher PEG MW. Moreover, the release behaviors of conjugates showed a pH-sensitive property. In vivo pharmacokinetic studies demonstrated that the elimination half-life was prolonged in comparison with ORI solution. PEGylation could be a promising method to obtain better efficacy in the field of drug delivery system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpharm.2013.08.014DOI Listing
November 2013

Nanosuspension for parenteral delivery of a p-terphenyl derivative: preparation, characteristics and pharmacokinetic studies.

Colloids Surf B Biointerfaces 2013 Aug 14;108:29-33. Epub 2013 Mar 14.

Department of Pharmaceutics, College of Pharmacy, Shandong University, 44 Wenhua Xilu, Jinan 250012, PR China.

Recently, nanosuspension technology has evolved into a mature drug delivery system, which can enhance the saturation solubility and dissolution velocity of poorly soluble drugs. In this study, nanosuspensions of a p-terphenyl derivative (H2) were prepared by combining microfluidization and precipitation method and transformed into dry powder by lyophilization. The resultant nanosuspensions had a mean particle size of 201.7±5.87nm and a zeta potential of -21.07±0.57mV. The X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) analysis verified that the crystalline state of H2 was not transformed when it was prepared to nanosuspensions. An increased saturation solubility (1.46ug/ml) and accelerated dissolution velocity were achieved. The percent drug release of bulk H2 and H2 nanosuspension dried powder was 7.16% and 93.5% at 120min point, respectively. The pharmacokinetic test in rats indicated that the area under plasma concentration-time curve (AUC0~∞) value of H2 nanosuspension (5.183mg/Lh) was about 5-fold higher than that of H2 solution (1.094mg/Lh). Additionally, the mean retention time (MRT) value of H2 nanosuspension (2.832h) was significantly longer than that of H2 solution (0.997h).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2013.02.038DOI Listing
August 2013

Preparation and characterization of galactosylated bovine serum albumin nanoparticles for liver-targeted delivery of oridonin.

Int J Pharm 2013 May 19;448(1):79-86. Epub 2013 Mar 19.

College of Pharmacy, Shandong University, Jinan 250012, China.

In this study, galactosylated bovine serum albumin (GB), which could be developed for a liver targeting carrier was synthetized and it was identified by Fourier transform infrared (FT-IR) spectrometer. Oridonin loaded bovine serum albumin nanoparticle (ORI-BSA-NP) and oridonin loaded GB nanoparticle (ORI-GB-NP) were prepared and optimized by the desolvation technique. During the preparation of ORI-GB-NP, galactosamine was introduced to end-cap the free aldehyde groups on nanoparticles. The characteristics of ORI-GB-NP such as particle size, zeta potential, particle morphologie, entrapment efficiency and drug loading were evaluated. The nearly spherical nanoparticles, with a narrow size distribution below 200 nm, were negatively charged with zeta potential of about -30 mV. Meanwhile, differential scanning calorimetry (DSC) and X-ray diffraction confirmed the amorphous state of ORI in ORI-GB-NP. The in vitro drug release of ORI from ORI-GB-NP presented a biphasic pattern with an initial burst effect and consequently sustained release. These results implied that the nanoparticles possessed fine physicochemical characteristics and seemed to be a stable delivery system for poorly soluble oridonin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpharm.2013.03.019DOI Listing
May 2013

In vitro and in vivo evaluation of paclitaxel-loaded mesoporous silica nanoparticles with three pore sizes.

Int J Pharm 2013 Mar 4;445(1-2):12-9. Epub 2013 Feb 4.

Department of Pharmaceutics, College of Pharmacy, Shandong University, 44 Wenhua Xilu, Jinan 250012, PR China.

In the present study, mesoporous silica nanoparticles (MSNs) with three pore size were manufactured by the etch method. A typical chemotherapeutic agent, paclitaxel (PTX) was loaded into these MSNs. The in vitro drug release behavior, the in vitro anti-tumor activity, the morphological apoptosis cell changes, cell apoptosis rate and pharmacokinetics were extensively evaluated to clarify the biomedical roles of these MSNs in the application of drug delivery. The results showed that paclitaxel-loaded MSNs not only demonstrated effective drug loading but also exhibited pore-size-dependent drug release performance in vitro. In addition, MSNs exhibited pore-size-dependent anti-tumor activity against breast cancer MCF-7 cells. The apoptosis mechanism study demonstrated that the percentage of early and late apoptosis of all PTX-loaded MSNs treated MCF-7 cells were significantly higher than that of free PTX, and additionally the percentage of apoptosis for PTX-loaded MSNs increased as the pore size of carriers enlarged. The pharmacokinetics results showed that PTX-loaded MSNs with the largest pore size exhibited the pharmacokinetic property similar to the PTX solution and the other drug loaded MSNs displayed sustained release behavior. These results demonstrate that MSNs could be a very promising drug delivery system for pore-size controllable drug release and enhancing the anti-tumor activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpharm.2013.01.058DOI Listing
March 2013

In vitro and in vivo evaluation of riccardin D nanosuspensions with different particle size.

Colloids Surf B Biointerfaces 2013 Feb 11;102:620-6. Epub 2012 Sep 11.

Department of Pharmaceutics, School of Pharmaceutical Sciences, Shandong University, 44 Wenhua Xilu, Jinan 250012, PR China.

Riccardin D (RD) is a novel compound extracted from Chinese liverwort Marchantia polymorpha L. It exhibits various anticancer activities and can be used during lung cancer treatment. However, the compound's low solubility hinders its development. Recently nanosuspension has been developed as one of the most promising formulations for poorly water-soluble drugs. In order to understand the dissolution behavior of riccardin D in vitro and in vivo, two nanosuspensions of riccardin D with markedly different sizes were prepared. The particle size of nanosuspension A prepared by bottom-up method was 184.1±3.15 nm, while that of nanosuspension B prepared by top-down method was 815.4±9.65 nm. The main purpose of this study was to investigate the effects of particle size on pharmacokinetics and tissue distribution after intravenous administration. Riccardin D dissolving in organic solution was studied as control group. In pharmacokinetics study in Wistar rats, nanosuspension A showed properties similar to the control group, while nanosuspension B exhibited rather different properties. In tissue distribution research on Kunming strain mice, nanosuspension A had a multi-peak phenomenon because of reticulate endothelial system (RES) while nanosuspension B showed a high uptake in RES organs that passively target to the lungs. In conclusion, particle size of riccardin D nanosuspensions had obvious effects on pharmacokinetics and tissue distribution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.colsurfb.2012.09.006DOI Listing
February 2013

Successfully tailoring the pore size of mesoporous silica nanoparticles: exploitation of delivery systems for poorly water-soluble drugs.

Int J Pharm 2012 Dec 16;439(1-2):81-91. Epub 2012 Oct 16.

Department of Pharmaceutics, College of Pharmacy, Shandong University, 44 Wenhua Xilu, Jinan250012, PR China.

A novel approach was applied to fabricate mesoporous silica nanoparticles (MSNs) with different pore size in this study. The pore size of MSNs can be modulated conveniently from 3 nm to 10nm by controlling the etching time of MSNs with the NaBH(4) solution. The as-synthesized MSNs were investigated as carriers for loading and delivery of the model drug paclitaxel (PTX). The characteristics, drug loading capacity, in vitro drug release behavior, anti-tumor activity and the mechanism of cell uptake were systematically studies. The resultant MSNs showed uniform and mono-dispersed sphere with high drug loading capacity (12-21%). The in vitro drug release exhibited that the released rate of PTX from MSNs could be controlled by the pore size and the larger the pore size, the faster the release rate of PTX. The in vitro anti-tumor studies demonstrated that PTX-loaded MSNs produced higher cytotoxicity than free PTX. Besides, the PTX-loaded MSNs with largest pore size showed the highest anti-tumor activity. These results indicated that these MSNs could provide a promising platform for delivering water-insoluble drugs, controlling the release rate of drugs and increasing the anti-tumor activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijpharm.2012.10.011DOI Listing
December 2012
-->