Publications by authors named "Jingxiu Xu"

4 Publications

  • Page 1 of 1

Redox-sensitive CDC-42 clustering promotes wound closure in C. elegans.

Cell Rep 2021 Nov;37(8):110040

Center for Stem Cell and Regenerative Medicine and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China; Zhejiang University-University of Edinburgh Institute, Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address:

Tissue damage induces immediate-early signals, activating Rho small GTPases to trigger actin polymerization essential for later wound repair. However, how tissue damage is sensed to activate Rho small GTPases locally remains elusive. Here, we found that wounding the C. elegans epidermis induces rapid relocalization of CDC-42 into plasma membrane-associated clusters, which subsequently recruits WASP/WSP-1 to trigger actin polymerization to close the wound. In addition, wounding induces a local transient increase and subsequent reduction of HO, which negatively regulates the clustering of CDC-42 and wound closure. CDC-42 CAAX motif-mediated prenylation and polybasic region-mediated cation-phospholipid interaction are both required for its clustering. Cysteine residues participate in intermolecular disulfide bonds to reduce membrane association and are required for negative regulation of CDC-42 clustering by HO. Collectively, our findings suggest that HO-regulated fine-tuning of CDC-42 localization can create a distinct biomolecular cluster that facilitates rapid epithelial wound repair after injury.
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http://dx.doi.org/10.1016/j.celrep.2021.110040DOI Listing
November 2021

Wounding triggers MIRO-1 dependent mitochondrial fragmentation that accelerates epidermal wound closure through oxidative signaling.

Nat Commun 2020 02 26;11(1):1050. Epub 2020 Feb 26.

Center for Stem Cell and Regenerative Medicine and Department of Cardiology of The Second Affiliated Hospital, Zhejiang University School of Medicine, 310058, Hangzhou, China.

Organisms respond to tissue damage through the upregulation of protective responses which restore tissue structure and metabolic function. Mitochondria are key sources of intracellular oxidative metabolic signals that maintain cellular homeostasis. Here we report that tissue and cellular wounding triggers rapid and reversible mitochondrial fragmentation. Elevated mitochondrial fragmentation either in fzo-1 fusion-defective mutants or after acute drug treatment accelerates actin-based wound closure. Wounding triggered mitochondrial fragmentation is independent of the GTPase DRP-1 but acts via the mitochondrial Rho GTPase MIRO-1 and cytosolic Ca. The fragmented mitochondria and accelerated wound closure of fzo-1 mutants are dependent on MIRO-1 function. Genetic and transcriptomic analyzes show that enhanced mitochondrial fragmentation accelerates wound closure via the upregulation of mtROS and Cytochrome P450. Our results reveal how mitochondrial dynamics respond to cellular and tissue injury and promote tissue repair.
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http://dx.doi.org/10.1038/s41467-020-14885-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044169PMC
February 2020

Spatiotemporal expression pattern of Sjfz7 and its expression comparison with other frizzled family genes in developmental stages of Schistosoma japonicum.

Gene Expr Patterns 2019 06 6;32:44-52. Epub 2019 Mar 6.

Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, China. Electronic address:

Wnts are secreted signaling molecules that are implicated in a variety of growth-related processes. Frizzled proteins have been identified as receptors for Wnt ligands in vertebrates and invertebrates, but a functional role for dioecious flatworm Frizzleds has not been determined. To evaluate the endogenous role of Frizzled proteins during development, we have identified and characterized a Schistosoma japonicum frizzled gene (Sjfz7). We found that Sjfz7 encodes a 698 amino acid protein with typical characteristics of Frizzled proteins. The immunohistochemical localization pattern showed that Sjfz7 protein was extensively distributed in almost all tissues of S. japonicum, including subtegumental muscle cells, parenchymal cells, intestinal epithelial cells and male and female germ cells. This indicated that Sjfz7-mediated Wnt signaling might be associated with the development of musculature, intestinal tract and reproductive organs in schistosome. Comparing mRNA levels between frizzled family members showed that Sjfz7 mRNA was consistently higher in the developmental stages analyzed, suggesting that Sjfz7 may be responsible for more functional tasks than other frizzled family members. Comparing frizzled mRNA levels between not fully developed and normal worms suggested that Wnt signaling might be abnormal in not fully developed worms.
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http://dx.doi.org/10.1016/j.gep.2019.02.005DOI Listing
June 2019

Characterization and expression pattern of a novel Frizzled 8 receptor gene in Schistosoma japonicum.

Parasitol Int 2017 Oct 4;66(5):522-528. Epub 2017 Apr 4.

Northeast Agricultural University, College of Veterinary Medicine, Harbin 150030, People's Republic of China.

Wnt signaling as mediated by the Frizzled family receptors plays a vital role in the early development of animal embryos, organ formation, tissue regeneration and other physiological processes. In the present study, a novel Frizzled member, SjFz8, was isolated and characterized in Schistosoma japonicum. SjFz8 encodes an 1162-amino-acid protein with typical characteristics of Frizzled proteins. Quantitative real-time polymerase chain reaction analysis indicated that SjFz8 transcript level was highest in 7-day-old schistosomula. In adult stages, SjFz8 mRNA expression remained at a low level after male-female pairing. The immunohistochemical localization of the Fz8 protein revealed that it existed in almost all tissues of S. japonicum, including subtegumental muscle, parenchyma, oral suckers, ventral suckers, testes of the male and ovaries of the female. We speculated that the Wnt signaling pathway that was mediated by Fz8 might take part in regulating histogenesis and organogenesis during the schistosomulum period, and play an important role in regulating further growth and development of male and female worms.
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http://dx.doi.org/10.1016/j.parint.2017.04.001DOI Listing
October 2017
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