Publications by authors named "Jingwen Ma"

41 Publications

MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-β-Treated Endometrial Stromal Cells via the MAPK and Wnt/β-Catenin Signaling Pathways.

Pathol Oncol Res 2021 29;27:1609761. Epub 2021 Apr 29.

Department of Gynecology, General Hospital of Ningxia Medical University, Yinchuan, China.

Intrauterine adhesion (IUA) is one of the most prevalent reproductive system diseases in females. MicroRNAs (miRNAs) are reported to be master regulators in a variety of diseases, including IUA, but the role of microRNA-543 (miR-543) in IUA remains to be elucidated. In this study, we observed that miR-543 was downregulated in transforming growth factor-beta (TGF-β)-treated endometrial stromal cells (ESCs). Functionally, we observed that miR-543 suppressed the migration, epithelial-to-mesenchymal transition (EMT), and inhibited expression of extracellular matrix (ECM) proteins in TGF-β-treated ESCs. Mechanistically, MAPK1 is targeted by miR-543 after prediction and screening. A luciferase reporter assay demonstrated that miR-543 complementarily binds with the 3' untranslated region of mitogen-activated protein kinase 1 (MAPK1), and western blot analysis indicated that miR-543 negatively regulates MAPK1 protein levels. In addition, results from rescue assays showed that miR-543 inhibits the migration and EMT of TGF-β-treated ESCs by targeting MAPK1. In addition, we observed that miR-543 inactivates the Wnt/β-catenin signaling pathway through inhibiting the phosphorylation of MAPK1 and β-catenin. Finally, we confirmed that miR-543 represses migration, EMT and inhibits levels of ECM proteins in TGF-β-treated ESCs by targeting the Wnt/β-catenin signaling pathway. Our results demonstrated that miR-543 suppresses migration and EMT of TGF-β-treated ESCs by targeting the MAPK and Wnt/β-catenin pathways.
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http://dx.doi.org/10.3389/pore.2021.1609761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262167PMC
April 2021

Nitrogen-carbon materials base on pyrolytic graphene hydrogel for oxygen reduction.

J Colloid Interface Sci 2021 Jun 8;602:274-281. Epub 2021 Jun 8.

Lab of Advanced Nano-structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin 300354, China; Chemistry and Chemical Engineering Guangdong Laboratory, Shantou 515031 China. Electronic address:

Hypothesis: Oxygen reduction reaction (ORR) has played a significant role in the utilization of energy nowadays. Nitrogen-doped carbon materials are seen as promising catalysts for ORR, so it is of great significance in studying the functions of different nitrogen moieties.

Experiments: The graphene hydrogel-based nitrogen-arbon materials (GH N-C) were fabricated by first obtaining a gel through hydrothermal treatment using graphene oxide (GO) as precursor, and then calcined in an ammonia atmosphere at different temperatures to form N-doped graphitized materials with divers nitrogen configuration.

Findings: GH N-C materials with tunable nitrogen configuration were synthesized by a two-step method base on graphene hydrogel. Benefiting from the 3D hydrogel structure, rich defects and optimized chemical properties, GH N-C-900 prepared by NH pyrolysis at 900 °C exhibits an excellent electrocatalytic performance toward ORR, with the onset potential of 0.947 ± 0.013 V versus RHE, half-wave potential of 0.830 ± 0.010 V versus RHE, electron transfer number of 3.61-3.99, along as methanol tolerance and superior long-term stability. Comprehensive studies have shown that there is a positive correlation between the total amount of pyrrolic-N and quaternary-N and the catalytic performance of ORR.
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http://dx.doi.org/10.1016/j.jcis.2021.06.036DOI Listing
June 2021

The PI3K/Akt Pathway: Emerging Roles in Skin Homeostasis and a Group of Non-Malignant Skin Disorders.

Cells 2021 May 17;10(5). Epub 2021 May 17.

Department of Dermatology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310014, China.

The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway regulates cell proliferation, differentiation, and migration, along with angiogenesis and metabolism. Additionally, it could mediate skin development and homeostasis. There is much evidence to suggest that dysregulation of PI3K/Akt pathway is frequently associated with several human cutaneous malignancies like malignant melanoma (MM), basal cell carcinoma (BCC), and cutaneous squamous cell carcinoma (SCC), as well as their poor outcomes. Nevertheless, emerging roles of PI3K/Akt pathway cascade in a group of common non-malignant skin disorders including acne and psoriasis, among others, have been recognized. The enhanced understanding of dysfunction of PI3K/Akt pathway in patients with these non-malignant disorders has offered a solid foundation for the progress of updated therapeutic targets. This article reviews the latest advances in the roles of PI3K/Akt pathway and their targets in the skin homeostasis and progression of a wide range of non-malignant skin disorders and describes the current progress in preclinical and clinical researches on the involvement of PI3K/Akt pathway targeted therapies.
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http://dx.doi.org/10.3390/cells10051219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156939PMC
May 2021

Nanomechanical topological insulators with an auxiliary orbital degree of freedom.

Nat Nanotechnol 2021 May 19;16(5):576-583. Epub 2021 Apr 19.

Department of Electronic Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.

Discrete degrees of freedom, such as spin and orbital, provide a tool to manipulate electrons, photons and phonons. Topological insulators have stimulated intense interests in condensed-matter physics, optics, acoustics and mechanics, usually with a focus on the spin degree of freedom. However, the orbital degree of freedom constitutes another fundamental attribute in crystals, but has seldom been investigated in topological insulators. Here, we demonstrate topological insulators with an auxiliary orbital degree of freedom on a nanomechanical platform. We realize an adiabatic transition between distinct topological edge states, which constitutes a crucial functionality for integrated circuits accommodating distinct topological edge channels. Beyond the one-dimensional edge states, we further construct zero-dimensional Dirac-vortex states using the orbital degree of freedom. These nanomechanical Dirac-vortex states exhibit strong second-order and third-order nonlinearities. Our results introduce the orbital degree of freedom as an alternative means to manipulate the topological phase transition on an integrated platform.
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http://dx.doi.org/10.1038/s41565-021-00868-6DOI Listing
May 2021

The Differences in the Whole-Brain Functional Network between Cantonese-Mandarin Bilinguals and Mandarin Monolinguals.

Brain Sci 2021 Mar 2;11(3). Epub 2021 Mar 2.

Department of Maternal and Child Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, Guangdong, China.

Cantonese-Mandarin bilinguals are logographic-logographic bilinguals that provide a unique population for bilingual studies. Whole brain functional connectivity analysis makes up for the deficiencies of previous bilingual studies on the seed-based approach and helps give a complete picture of the brain connectivity profiles of logographic-logographic bilinguals. The current study is to explore the effect of the long-term logographic-logographic bilingual experience on the functional connectivity of the whole-brain network. Thirty Cantonese-Mandarin bilingual and 30 Mandarin monolingual college students were recruited in the study. Resting state functional magnetic resonance imaging (rs-fMRI) was performed to investigate the whole-brain functional connectivity differences by network-based statistics (NBS), and the differences in network efficiency were investigated by graph theory between the two groups (false discovery rate corrected for multiple comparisons, = 0.05). Compared with the Mandarin monolingual group, Cantonese-Mandarin bilinguals increased functional connectivity between the bilateral frontoparietal and temporal regions and decreased functional connectivity in the bilateral occipital cortex and between the right sensorimotor region and bilateral prefrontal cortex. No significant differences in network efficiency were found between the two groups. Compared with the Mandarin monolinguals, Cantonese-Mandarin bilinguals had no significant discrepancies in network efficiency. However, the Cantonese-Mandarin bilinguals developed a more strongly connected subnetwork related to language control, inhibition, phonological and semantic processing, and memory retrieval, whereas a weaker connected subnetwork related to visual and phonology processing, and speech production also developed.
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http://dx.doi.org/10.3390/brainsci11030310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000089PMC
March 2021

Experimental Demonstration of Dual-Band Nano-Electromechanical Valley-Hall Topological Metamaterials.

Adv Mater 2021 Mar 4;33(10):e2006521. Epub 2021 Feb 4.

Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

Suppression of undesired backscattering of very-high-frequency elastic signals has been considered as a grand challenge in integrated phononic circuits. Originating from condensed-matter physics, valley-Hall topological insulators provide an intriguing strategy to overcome this challenge. To date, phononic valley-Hall topological insulators have been demonstrated only in bulk acoustic and mechanical systems operating at relatively low frequencies. Here, an integrated nano-electromechanical valley-Hall topological insulator operating in the very-high-frequency regime is experimentally realized. Valley kink states that are backscattering-immune against sharp bends and exhibit the "valley-momentum locking" effect simultaneously in the fundamental (≈60 MHz) and second-order (≈120 MHz) frequency bands are demonstrated. It is further shown that the propagation directions of these dual-band valley kink states are always locked to their valley pseudospins. The results not only enable various applications in very-high-frequency integrated phononic circuits with enhanced robustness and capacity, but also open the door to experimental exploration of mechanical nonlinearities, particularly those involving the fundamental and second-order frequencies, in topologically nontrivial nanostructures.
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http://dx.doi.org/10.1002/adma.202006521DOI Listing
March 2021

Observation of chiral edge states in gapped nanomechanical graphene.

Sci Adv 2021 Jan 6;7(2). Epub 2021 Jan 6.

Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.

Emerging in diverse areas of physics, edge states have been exploited as an efficient strategy of manipulating electrons, photons, and phonons for next-generation hybrid electro-optomechanical circuits. Among various edge states, gapless chiral edge states harnessing quantum spin/valley Hall effects in graphene or graphene-like materials are especially unique. Here, we report on an experimental demonstration of chiral edge states in gapped "nanomechanical graphene"-a honeycomb lattice of free-standing silicon nitride nanomechanical membranes with broken spatial inversion symmetry. These chiral edge states can emerge from the conventional flat-band edge states by tuning the on-site boundary potentials. We experimentally demonstrated that they are backscattering-immune against sharp bends and exhibit the "valley-momentum locking" effect. We further realized smooth transition between the chiral edge states and the well-known valley kink states. Our results open the door to experimental investigation of exotic graphene-related physics in the very-high-frequency integrated nanomechanical systems.
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http://dx.doi.org/10.1126/sciadv.abe1398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787500PMC
January 2021

Brain Activity during Visual and Auditory Word Rhyming Tasks in Cantonese-Mandarin-English Trilinguals.

Brain Sci 2020 Dec 4;10(12). Epub 2020 Dec 4.

Department of Maternal and Child Health, School of Public Health, Sun Yat-sen University, Guangzhou 510080, China.

It is unclear whether the brain activity during phonological processing of second languages (L2) is similar to that of the first language (L1) in trilingual individuals, especially when the L1 is logographic, and the L2s are logographic and alphabetic, respectively. To explore this issue, this study examined brain activity during visual and auditory word rhyming tasks in Cantonese-Mandarin-English trilinguals. Thirty Chinese college students whose L1 was Cantonese and L2s were Mandarin and English were recruited. Functional magnetic resonance imaging (fMRI) was conducted while subjects performed visual and auditory word rhyming tasks in three languages (Cantonese, Mandarin, and English). The results revealed that in Cantonese-Mandarin-English trilinguals, whose L1 is logographic and the orthography of their L2 is the same as L1-i.e., Mandarin and Cantonese, which share the same set of Chinese characters-the brain regions for the phonological processing of L2 are different from those of L1; when the orthography of L2 is quite different from L1, i.e., English and Cantonese who belong to different writing systems, the brain regions for the phonological processing of L2 are similar to those of L1. A significant interaction effect was observed between language and modality in bilateral lingual gyri. Regions of interest (ROI) analysis at lingual gyri revealed greater activation of this region when using English than Cantonese and Mandarin in visual tasks.
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http://dx.doi.org/10.3390/brainsci10120936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761916PMC
December 2020

ZFAS1 Exerts an Oncogenic Role via Suppressing miR-647 in an mA-Dependent Manner in Cervical Cancer.

Onco Targets Ther 2020 17;13:11795-11806. Epub 2020 Nov 17.

Gastrointestinal Department, General Hospital of Ningxia Medical University, Yinchuan City, Ningxia Province, People's Republic of China.

Background: Cervical cancer (CC) is the second serious health threat in women worldwide. LncRNA ( antisense RNA 1) has been observed to abnormally express in human cancers. However, the expression pattern, clinical significance and molecular mechanism of ZFAS1 have not been thoroughly studied in CC.

Methods: qRT-PCR was performed to examine the differential expression of ZFAS1 in CC tissues and adjacent normal cervical tissues. Gain- and loss-of-function experiments were constructed to test the functional role of ZFAS1 in CC by CCK-8, colony formation, transwell and xenograft models assays. Luciferase reporter, RNA immunoprecipitation (RIP), methylated RNA immunoprecipitation (MeRIP), RNA pull-down assays were used to reveal the underlying mechanisms.

Results: We found that ZFAS1 was significantly upregulated in CC tissues. Elevation of ZFAS1 correlated with advanced FIGO stage, lymph node and distant metastasis, and also indicated poor overall survival in patients with CC. Functional experiments demonstrated that ZFAS1 promoted CC cell proliferation, migration and invasion in vitro, and facilitated tumor growth and metastasis in vivo. Mechanistic investigation revealed that ZAFS1 sequestered miR-647, and this RNA-RNA interaction is regulated by METLL3-mediated mA modification.

Conclusion: Our findings elucidate the functional roles of ZFAS1 and its mA modification in CC cells and indicate that ZFAS1 may be a promising target for CC treatment.
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http://dx.doi.org/10.2147/OTT.S274492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680607PMC
November 2020

Silencing microRNA-210 in Hypoxia-Induced HUVEC-Derived Extracellular Vesicles Inhibits Hemangioma.

Cerebrovasc Dis 2020 2;49(5):462-473. Epub 2020 Sep 2.

Department of Dermatology, Zhejiang Provincial People's Hospital, Hangzhou, China.

Background: Hemangioma (Hem) is a benign tumor commonly seen in infancy with a relative high morbidity. Human umbilical vein endothelial cell (HUVEC)-derived extracellular vesicles (EVs) are actively participated in Hem. Therefore, this study is designed to figure out the underlying mechanism of HUVEC-derived EVs in Hem.

Methods: Initially, EVs were separated from HUVECs and identified. HUVEC-derived EVs in normoxia or hypoxia were then cultivated with Hem endothelial cells (HemECs) to test the proliferation, apoptosis, and migration of HemECs. Microarray analysis was performed to select microRNAs (miRs) with differential expression. miR-210 in hypoxia-induced HUVECs was silenced, and the relevant EVs were extracted and then co-cultured with HemECs to perform biological effect experiments. Then, the target relation between miR-210 and homeobox A9 (HOXA9) was identified by the dual luciferase reporter gene assay and RNA immunoprecipitation assay. Moreover, xenograft transplantation was also applied to confirm the in vitro experiments.

Results: Hypoxia-induced HUVECs promoted release of EVs, which were absorbed by HemECs. Hypoxia-induced HUVEC-EVs promoted HemEC proliferation and migration and inhibited apoptosis. miR-210 from the hypoxia-induced HUVEC-EVs was highly expressed and promoted HemEC growth. Silencing miR-210 expression in the hypoxia-induced HUVEC-EVs suppresses Hem development in vivo. In addition, miR-210 targeted HOXA9.

Conclusion: Silencing miR-210 in HUVEC-derived EVs could suppress Hem by targeting HOXA9. This investigation may provide novel insights for Hem treatment.
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http://dx.doi.org/10.1159/000508302DOI Listing
November 2020

Estrogen attenuates TGF-β1-induced EMT in intrauterine adhesion by activating Wnt/β-catenin signaling pathway.

Braz J Med Biol Res 2020 6;53(8):e9794. Epub 2020 Jul 6.

College of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, China.

Although estrogen has crucial functions for endometrium growth, the specific dose and underlying molecular mechanism in intrauterine adhesion (IUA) remain unclear. In this study, we aimed to investigate the effects of estrogen on epithelial-mesenchymal transition (EMT) in normal and fibrotic endometrium, and the role of estrogen and Wnt/β-catenin signaling in the formation of endometrial fibrosis. CCK-8 and immunofluorescence assay were performed to access the proliferation of different concentrations of estrogen on normal human endometrial epithelial cells (hEECs). qRT-PCR and western blot assay were utilized to explore the effect of estrogen on EMT in normal and fibrotic endometrium, and main components of Wnt/β-catenin signaling pathway in vitro. Hematoxylin and eosin and Masson staining were used to evaluate the effect of estrogen on endometrial morphology and fibrosis in vivo. Our results indicated that the proliferation of normal hEECs was inhibited by estrogen at a concentration of 30 nM accompanied by upregulation of mesenchymal markers and downregulation of epithelial markers. Interestingly, in the model of transforming growth factor β1 (TGF-β1)-induced endometrial fibrosis, the same concentration of estrogen inhibited the process of EMT, which might be partially mediated by regulation of the Wnt/β-catenin pathway. In addition, relatively high doses of estrogen efficiently increased the number of endometrial glands and reduced the area of fibrosis as determined by the reduction of EMT in IUA animal models. Taken together, our results demonstrated that an appropriate concentration of estrogen may prevent the occurrence and development of IUA by inhibiting the TGF-β1-induced EMT and activating the Wnt/β-catenin pathway.
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http://dx.doi.org/10.1590/1414-431x20209794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346761PMC
August 2020

Effects of low doses of UV-B radiation supplementation on tuber quality in purple potato ( L.).

Plant Signal Behav 2020 09 24;15(9):1783490. Epub 2020 Jun 24.

College of Life Science, Henan Normal University , Xinxiang, China.

UV-B is an important environmental factor that differentially affects plant growth and secondary metabolites. However, our knowledge regarding the physiological and biochemical changes in under-ground plant organs responded to UV-B treatment remains limited. In this study, we investigated potato plant ( L.) and tuber responses to short-term supplemental UV-B exposure performed during tuber development. Our results indicated that the supplemental UV-B radiation with relative low dose had no obvious adverse impact on plant growth or tuber production. Nutritional composition analyses of tubers revealed that the contents of starch, soluble sugars, and proteins were significantly increased under lower UV-B radiation relative to controls. Similarly, low dose of UV-B treatment promoted the health-promoting compounds, including anthocyanin, phenols, and flavonoids in tubers. Moreover, higher activities of antioxidant enzymes were significantly induced in tubers in response to lower UV-B radiation. These findings suggest that short-term UV-B radiation supplementation at relative low doses can improve the tuber quality in potato plants.
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http://dx.doi.org/10.1080/15592324.2020.1783490DOI Listing
September 2020

Hyperuricemia is associated with androgenetic alopecia in men: A cross-sectional case-control study.

J Cosmet Dermatol 2020 Nov 12;19(11):3122-3126. Epub 2020 Apr 12.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Early androgenetic alopecia (AGA) is patterned hair loss occurring before 30 years. Early AGA is frequently reported in men and carries the risk of obesity, metabolic syndrome, and cardiovascular diseases. Hyperuricemia used to be a minor component of metabolic syndrome. Recently, increasing number of studies has proved that hyperuricemia is an independent risk factor for many cardiovascular diseases and psoriasis. However, none of these studies have examined the relationship between hyperuricemia and AGA.

Aims: To determine the association between hyperuricemia and AGA in men.

Methods: A cross-sectional case-control study was conducted. The medical charts and photographs of men with a clinical diagnosis of AGA were reviewed. The clinical and laboratory data of AGA and control groups were compared.

Results: Men with AGA (n = 1312) had higher mean uric acid level (6.25 mg/dL vs 5.97 mg/dL; P < .001) and higher prevalence of hyperuricemia (25.0% vs 15.6%; P < .001) than those without AGA (n = 2624). There was no statistically significant association between AGA severity and hyperuricemia (P = .295).

Conclusions: Men with early AGA have a higher prevalence of hyperuricemia.
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http://dx.doi.org/10.1111/jocd.13401DOI Listing
November 2020

Neural substrates of bilingual processing in a logographic writing system: An fMRI study in Chinese Cantonese-Mandarin bilinguals.

Brain Res 2020 07 28;1738:146794. Epub 2020 Mar 28.

Department of Maternal and Child Health, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address:

Logographic-logographic bilingualism is lacking in bilingual researches. Cantonese and Mandarin are two Chinese languages that belong to the logographic writing system and share the same set of Chinese characters. Cantonese-Mandarin bilinguals employ logographic-logographic bilingualism, and thus provide a unique population for bilingual studies. In Cantonese-Mandarin bilinguals, it remains unclear whether the second language (L2) phonological processing conforms to the assimilation or accommodation hypothesis and whether the brain mechanisms underlying Cantonese and Mandarin phonological processing are similar. Differential brain mechanisms of Mandarin as first language (L1) and L2 also needs to be clarified. Task-modality functional magnetic resonance imaging (fMRI) was used to explore these questions in Cantonese-Mandarin bilinguals and native Mandarin speakers. Univariate analysis, activation similarity analysis, brain-behavioral analysis and psychophysiological interaction (PPI) analysis were conducted. Novel evidence for accommodation was observed in the L2 processing of Cantonese-Mandarin bilinguals, indicating that small language distance leads to the accommodation of L2 processing, which is absolutely distinct to alphabetic bilingualism. We speculated that the role of language distance in the balance between assimilation and accommodation may be regulated by L1. Notably, we found Cantonese processing relied more on orthography-to-phonology mapping, phonological processing, language control and inhibition while Mandarin relied more on memory extraction. And Mandarin processing as L1 and L2 in our study are highly similar, suggesting the stability of brain regions processing Mandarin.
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http://dx.doi.org/10.1016/j.brainres.2020.146794DOI Listing
July 2020

The intronic promoter of Actin4 mediates high-level transgene expression mainly in the wing and epidermis of silkworms.

Transgenic Res 2020 04 20;29(2):243-251. Epub 2020 Jan 20.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

The cytoplasmic actin gene Actin4 (A4) in silkworm (Bombyx mori) was isolated 20 years ago and has a distal promoter upstream of the first exon and a proximal promoter within the first intron; however, how the promoter regulates gene expression has yet to be fully elucidated. Here, we characterized the function and expression of the proximal promoter (named A4IP) by analyzing transgenic Gal4/UAS silkworms, A4IP-Gal4/UAS-EGFP. We demonstrated that A4IP drives the expression of Gal4 and thereby activates UAS-linked EGFP in transgenic silkworms beginning in day-3 embryos through adults. Further detection revealed that EGFP was expressed at a low level in tissues including the trachea, fat body and midgut but was highly expressed in the wing disks/wings and inner epidermis of transgenic silkworms. No EGFP signals were detected in other tissues by western blot assay. Interestingly, EGFP fluorescence had a spot-like distribution on the epidermis of transgenic larvae. These observations are quite different from those in transgenic silkworms driven by the promoter of Actin3 (A3), another cytoplasmic actin gene in B. mori. These findings reveal the expression profiles of the A4IP promoter and provide new insights into the regulatory mechanism of cytoplasmic actin genes in silkworms.
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http://dx.doi.org/10.1007/s11248-020-00192-0DOI Listing
April 2020

Amplification of 18 OAM modes in a ring-core erbium-doped fiber with low differential modal gain.

Opt Express 2019 Dec;27(26):38087-38097

We theoretically propose two orbital angular momentum (OAM) erbium-doped ring-core fiber (RCF) amplifiers capable of providing relatively uniform gain for 22 modes with 18 OAM ones over the C-band. Two schemes of doping profile are discussed, one with single layer erbium doping and the other with double layer erbium doping. Theoretical analyses and numerical simulations suggest that the proposed first OAM erbium-doped fiber amplifier (OAM-EDFA) can obtain a gain larger than 20 dB for all 22 modes with differential modal gain (DMG) lower than 0.71 dB. The second OAM-EDFA performs better and can provide a larger gain over 21.5 dB for all 22 modes, with a smaller DMG below 0.27 dB and the noise figure (NF) lower than 3.9 dB over the whole C band. The presented OAM-EDFA may open up new perspectives for long-distance transmission in capacity scaling fiber-optic communications using OAM modes.
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http://dx.doi.org/10.1364/OE.27.038087DOI Listing
December 2019

Insights into regulatory characteristics of the promoters of Sericin 1 and Sericin 3 in transgenic silkworms.

Biochem Biophys Res Commun 2020 02 25;522(2):492-498. Epub 2019 Nov 25.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; College of Biotechnology, Southwest University, Chongqing, China. Electronic address:

Sericin, produced in the middle silk gland (MSG) of silkworms, is a group of glue proteins that coat and cement silk fibers. Several genes are known to encode sericin, but their spatiotemporal regulation has yet to be fully elucidated. Here, we report in detail the expression profiles of the promoters of two major sericin-coding genes, Sericin 1 (Ser1)and Sericin 3 (Ser3), by analyzing Gal4/UAS transgenic silkworms. We found that UAS-linked EGFP fluorescence in transgenic silkworms driven by Ser1-Gal4was detected in only the R3, R4 and R5 regions of MSG starting inday-3 fifth-instar larvae and was continuously expressed until silk gland degradation. In transgenic silkworms driven by Ser3-Gal4, EGFP fluorescence was detected at a low level in the R2 region of MSG since the last day of fifth-instar larvae, and the expression increased during the wandering stages and was continuously detected until silk gland degradation. The molecular detection of EGFP expression in each of the Gal4/UAS transgenic silkworms was consistent with fluorescence observations. These findings reveal clear differences in the regulatory characteristics of the promoters of Ser1and Ser3 and provide new insights into the regulatory mechanism of the expression of sericin-coding genes.
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http://dx.doi.org/10.1016/j.bbrc.2019.11.094DOI Listing
February 2020

PVA reinforced gossypolone and doxorubicin π-π stacking nanoparticles towards tumor targeting and ultralow dose synergistic chemotherapy.

Biomater Sci 2019 Aug;7(9):3662-3674

Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

To improve the tumor synergistic therapeutic effects of carrier-free dual-drug delivery systems and realize ultralow dose administration, we developed a tumor targeting and high-efficiency synergistic chemotherapy system ([email protected] NPs) based on polyvinyl alcohol (PVA) reinforced gossypolone (Gn) and doxorubicin (DOX) π-π stacking nanoparticles (DPGn NPs), in which PVA filled the gaps between Gn and DOX and bridged Gn and DOX tightly. Hyaluronic acid modifier hyaluronic acid-gossypolone (HA-Gn) was covered on the surface of DPGn NPs to form [email protected] NPs that procured active targeting properties. This system presented a spherical shape with a uniform hydrodynamic size of 87 ± 6.8 nm, a high drug loading of 80.31%, and high stability. FTIR and UV spectra demonstrated that HA-Gn was covered on the surface of the system and showed significant π-π stacking properties. A considerably low combination index of Gn and DOX (0.1862) was determined at an ultra-low dose of DOX under a Gn : DOX ratio of 50 : 1. [email protected] NPs also demonstrated excellent tumor synergistic therapeutic efficacy (TIR > 87%) at an ultralow dose of DOX and Gn. This system demonstrates high tumor comprehensive synergistic therapeutic efficacy at an ultralow drug dose with multiple favorable therapeutic characteristics, including negligible side effects, tumor targeting ability and thermal-responsive drug release, and thus has considerable potential for tumor synergistic therapy.
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http://dx.doi.org/10.1039/c9bm00674eDOI Listing
August 2019

Identification of blood microRNA alterations in patients with severe active alopecia areata.

J Cell Biochem 2019 09 15;120(9):14421-14430. Epub 2019 Apr 15.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Alopecia areata (AA) is a common inflammatory disease characterized by cellular infiltration of T cells targeting the anagen-stage hair follicle. Lack of efficacious treatment for AA may be due to little knowledge about its exact cellular mechanism. Studies have demonstrated that microRNAs (miRNAs) play an important role in the regulation of inflammatory skin diseases such as atopic dermatitis and psoriasis. However, little is known about the role of miRNAs in AA.

Objective: The present study aimed to explore the blood miRNAs alterations in patients with severe active AA.

Methods: We constructed a bipartite miRNA-messenger RNA (mRNA) regulatory network by the validated miRNA-mRNA relationships. Subsequently, the miRNA-miRNA synergistic network was formed in consideration of the Gene Ontology function enrichment of coregulated target genes. Lastly, the functional network was identified by the ingenuity pathway analysis.

Results: By using an Agilent microarray that covers 2549 human miRNAs, we identified 36 significantly differentially expressed miRNAs in severe active AA patients. miRNA target gene prediction and functional annotation analysis showed significant enrichment in several pathways including the ribosome, cancer, cell cycle, insulin signaling, transforming growth factor-βsignaling, and p53 signaling pathways. Analysis of the three kinds of network showed that miR-185-5p, miR-125b-5p, and miR-186-5p might play important and synergistic roles in the active phase of AA. According to the receiver operating characteristic curve analysis, several miRNAs were selected for the quantitative real-time polymerase chain reaction validation. Among the miRNAs, miR-210 and miR-1246 had high prediction with high accuracy.

Conclusion: Blood dysregulated miRNAs are potentially associated with the severe active AA. These miRNAs could function synergistically and might be promising targets for the development of novel treatments for AA in the future.
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http://dx.doi.org/10.1002/jcb.28700DOI Listing
September 2019

Redox-Responsive Dual Chemophotothermal Therapeutic Nanomedicine for Imaging-Guided Combinational Therapy.

J Mater Chem B 2018 Sep 23;6(33):5362-5367. Epub 2018 Jul 23.

State key Laboratory of Natural medicines, Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.

Chemotherapy is currently the major therapeutic method against cancer. However, chemo drugs are usually lacking in the specificity towards cancer cells over normal cells. In this study, we prepared a novel multifunctional trimeric prodrug by linking the chemo drug camptothecin (CPT) and the exceptional photostable near infrared (NIR) croconaine dye (CR) a glutathione (GSH)-sensitive disulfide linker. Compared with CPT, our novel trimeric CR-(SS-CPT) is more hydrophobic and bulky, making it highly effcient to be encapsulated into biocompatible polymeric nanocarrier. The prodrug underwent rapid drug release specifically in cancer cells with high GSH concentration. The hyperthermia produced by CR upon laser irradiation could further accelerate the break of disulfide bond, which makes the release of CPT even more controllable. We further loaded the CR-(SS-CPT) into folate modified lipid-polymer nanoparticles, which demonstrated high tumor accumulation and retention. The biodistribution of these nanoparticles can be directly monitored in real time NIR fluorescence and photoacoustic imaging. Under the imaging guided chemo- and photothermal- synergistic therapy, the tumors were completely ablated with no recurrence. The design not only highly enhanced the therapeutic specificity and effeciency of CPT, but also provide a "all in one" nanomedicine for imaging-guided dual modality therapy.
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http://dx.doi.org/10.1039/C8TB01360HDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435337PMC
September 2018

Differential expression patterns of specific long noncoding RNAs and competing endogenous RNA network in alopecia areata.

J Cell Biochem 2019 06 20;120(6):10737-10747. Epub 2019 Feb 20.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China.

Background: Long noncoding RNAs (lncRNAs) regulate gene expression by acting with microRNAs (miRNAs) and indirectly interact with messenger RNA (mRNAs). However, the roles of specific lncRNA and its related competing endogenous RNAs (ceRNA) network in alopecia areata (AA) are not fully understood.

Methods: The blood lncRNA profiles were obtained by microarray from 10 samples, including five alopecia areata samples and five normal samples. Based on bioinformatics generated from miRcode, starBase, and miRTarBase, we constructed an lncRNA-miRNA-mRNA network (ceRNA network) in alopecia areata.

Results: We found 154 differentially expressed lncRNAs and 46 differentially expressed genes (DEGs). The functional enrichment indicated that the DEGs mainly regulated the pathways of focal adhesion, Mucin type O-glycan biosynthesis, and so on. The differentially expressed lncRNA (DElncRNA) involved in the pathway of thyronamine and iodothyronamine metabolism and so on. Through integrated lncRNA-mRNA and miRNA-mRNA pairs, the ceRNA network was constructed, thereafter, six ceRNA subnetworks were identified and subnetwork 1 were found to be significantly associated with the occurrence of alopecia areata.

Conclusion: Our results showed blood lncRNA expression patterns and a complex ceRNA network in alopecia areata. However, futher studies on blood and tissue verification of these lncRNAs and relative pathways are needed.
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http://dx.doi.org/10.1002/jcb.28365DOI Listing
June 2019

3D self-supported Ni(PO)-MoO nanorods anchored on nickel foam for highly efficient overall water splitting.

Nanoscale 2018 Dec 27;10(47):22173-22179. Epub 2018 Nov 27.

Lab of Advanced Nano Structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin 300354, P. R. China.

Electrolyzing water as a sustainable energy source is a promising and appealing method to resolve the environmental crisis. Developing efficient and stable bifunctional electrocatalysts for the hydrogen evolution reaction (HER) and the oxygen evolution reaction (OER) is crucial and challenging in the overall water splitting process. Herein, we report the synthesis of Ni(PO)-MoO nanorods anchored on nickel foam (Ni(PO)-MoO/NF) within a two-step strategy and their application as a bifunctional water splitting electrocatalyst. The results show that the optimal Ni(PO)-MoO/NF electrodes exhibit superior catalytic activity with robust durability and ultralow overpotentials of 86 mV for HER and 234 mV for OER to achieve 10 mA cm (η) in alkaline solution. The favorable performance of the obtained catalyst is attributed mainly to the synergetic effect between Ni(PO) and MoO, as well as the self-supporting porous conductive substrate. As a result, the integrated Ni(PO)-MoO/NF electrodes deliver η at a small potential of 1.47 V for overall water splitting, highlighting a promising application as a bifunctional electrocatalyst.
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http://dx.doi.org/10.1039/c8nr07577hDOI Listing
December 2018

Hypoxia-activated prodrugs and redox-responsive nanocarriers.

Int J Nanomedicine 2018 18;13:6551-6574. Epub 2018 Oct 18.

Engineering Research Center of Molecular and Neuro Imaging of the Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an 710071, Shaanxi Province, People's Republic of China,

Hypoxia is one of the marked features of malignant tumors, which is associated with several adaptation changes in the microenvironment of tumor cells. Therefore, targeting tumor hypoxia is a research hotspot for cancer therapy. In this review, we summarize the developing chemotherapeutic drugs for targeting hypoxia, including quinones, nitroaromatic/nitroimidazole, N-oxides, and transition metal complexes. In addition, redox-responsive bonds, such as nitroimidazole groups, azogroups, and disulfide bonds, are frequently used in drug delivery systems for targeting the redox environment of tumors. Both hypoxia-activated prodrugs and redox-responsive drug delivery nanocarriers have significant effects on targeting tumor hypoxia for cancer therapy. Hypoxia-activated prodrugs are commonly used in clinical trials with favorable prospects, while redox-responsive nanocarriers are currently at the experimental stage.
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http://dx.doi.org/10.2147/IJN.S173431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202002PMC
December 2018

Magnetic Au-Ag-γ-Fe₂O₃/rGO Nanocomposites as an Efficient Catalyst for the Reduction of 4-Nitrophenol.

Nanomaterials (Basel) 2018 Oct 25;8(11). Epub 2018 Oct 25.

Lab of Advanced Nano-structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin 300354, China.

In this paper, a facile route has been developed to prepare magnetic trimetallic Au-Ag-γ-Fe₂O₃/rGO nanocomposites. The impact of the preparation method (the intensity of reductant) on the catalytic performance was investigated. The nanocomposites were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The prepared nanocomposites show fine catalytic activity towards the reduction reaction of 4-nitrophenol (4-NP). The nanocomposites also have superparamagnetism at room temperature, which can be easily separated from the reaction systems by applying an external magnetic field.
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http://dx.doi.org/10.3390/nano8110877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266811PMC
October 2018

Effect of 410 nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor (VEGF) in cultured human vascular endothelial cells.

Lasers Med Sci 2019 Feb 22;34(1):149-155. Epub 2018 Oct 22.

Department of Dermatology, Huashan Hospital, Fudan University, No. 12 Wulumuqi Zhong Road, Shanghai, 200040, China.

Photodynamic therapy (PDT) is considered an effective alternative for the treatment of port-wine stains (PWS) using hemoporfin (hematoporphyrin monomethyl ether, HMME), a novel photosensitizer with better efficacy and lower recurrence. Vascular endothelial growth factor (VEGF) plays an important role in the development of PWS. Therefore, we conducted this study to investigate the effect of HMME-PDT on VEGF expression. Human vascular endothelial cells (HUVECs) were treated with different doses of HMME and irradiated with 410-nm light emitting-diode (LED) light. To assess cell viability, CCK-8 assays were performed. At 48 h after PDT, the expression of VEGF/VEGF receptor (VEGFR) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Measurement of VEGF protein was carried out using western blotting assays. Cell viability was significantly inhibited after HMME-PDT and was dose-dependent within a certain range. HMME-PDT decreased secretion of VEGF 48 h after irradiation in HUVECs as compared to controls. The downregulation of VEGF and VEGFR mRNA as well as VEGF protein expression was more significant in the high HMME concentration group (4 μg/mL) than in the lower concentration group (2 μg/mL). Our outcomes provide evidence, that HMME-PDT can downregulate VEGF expression in cultured HUVECs and may explain the efficacy of hemoporfin PDT for PWS treatment.
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http://dx.doi.org/10.1007/s10103-018-2649-8DOI Listing
February 2019

Parity-time-symmetric mechanical systems by the cavity optomechanical effect.

Opt Lett 2018 Sep;43(17):4088-4091

Parity-time (PT) symmetry, an interesting concept originally introduced in quantum mechanics, has been extended to many other branches of physics. Besides its demonstrations in optics and electronics, the study of PT-symmetric mechanics is growing rapidly. To date, most PT-symmetric mechanical systems have relatively large size and low operating frequency, which limits their applications in high-speed sensing and signal processing. Here, we propose a PT-symmetric mechanical system with gain/loss provided by the cavity optomechanical effect, which can overcome those limitations. We theoretically analyze and numerically simulate the optical control of PT-symmetric optomechanical systems that consist of two or more mechanical resonators. We find the property of unidirectional reflection in these systems, which may pave the way for the study of topological acoustics and phononic signal processing.
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http://dx.doi.org/10.1364/OL.43.004088DOI Listing
September 2018

Preparation of Cuprous Oxide Mesoporous Spheres with Different Pore Sizes for Non-Enzymatic Glucose Detection.

Nanomaterials (Basel) 2018 Jan 29;8(2). Epub 2018 Jan 29.

Lab of Advanced Nano-Structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin 300354, China.

Mass transfer plays a significant role in a sensor's performance, because the substrate can be detected only when it contacts with the active catalytic surface. In this work, cuprous oxide mesoporous nanospheres (Cu₂O MPNS) with different pore size distributions are fabricated and applied as electrocatalysts for glucose detection. The small pore Cu₂O (SP-Cu₂O, mean pore size of 5.3 nm) and large pore Cu₂O (LP-Cu₂O, mean pore size of 16.4 nm) spheres are prepared by the template method and an etching treatment. The obtained two kinds of Cu₂O MPNS exhibit high porosity with a similar specific surface area of 61.2 and 63.4 (m²·g), respectively. The prepared Cu₂O MPNS are used to construct an electrochemical non-enzymatic glucose sensor. The results show that the LP-Cu₂O exhibits better performance than SP-Cu₂O, which illustrates that the internal diffusion takes a great impact on the performance of the sensor. The LP-Cu₂O modified electrode possesses a high and reproducible sensitivity of 2116.9 μA mM·cm at the applied potential of 0.6 V with a wide detection range of 0.003-7.8 mM and a low detection limit of 0.42 μM.
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http://dx.doi.org/10.3390/nano8020073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853705PMC
January 2018

Polyaniline Derived N-Doped Carbon-Coated Cobalt Phosphide Nanoparticles Deposited on N-Doped Graphene as an Efficient Electrocatalyst for Hydrogen Evolution Reaction.

Small 2018 01 17;14(2). Epub 2017 Nov 17.

Lab of Advanced Nano Structures & Transfer Processes, Department of Chemical Engineering, Tianjin University, Tianjin, 300354, P. R. China.

The development of highly efficient and durable non-noble metal electrocatalysts for the hydrogen evolution reaction (HER) is significant for clean and renewable energy research. This work reports the synthesis of N-doped graphene nanosheets supported N-doped carbon coated cobalt phosphide (CoP) nanoparticles via a pyrolysis and a subsequent phosphating process by using polyaniline. The obtained electrocatalyst exhibits excellent electrochemical activity for HER with a small overpotential of -135 mV at 10 mA cm and a low Tafel slope of 59.3 mV dec in 0.5 m H SO . Additionally, the encapsulation of N-doped carbon shell prevents CoP nanoparticles from corrosion, exhibiting good stability after 14 h operation. Moreover, the as-prepared electrocatalyst also shows outstanding activity and stability in basic and neutral electrolytes.
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http://dx.doi.org/10.1002/smll.201702895DOI Listing
January 2018

Natural Product Gossypol and its Derivatives in Precision Cancer Medicine.

Curr Med Chem 2019 ;26(10):1849-1873

The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China.

Gossypol, a natural product extracted from the seed, roots, and stem of cotton, was initially used as a male contraceptive but was subsequently investigated as a novel antitumor agent. This review depicts the current status of gossypol and its derivatives as novel antitumor agents as well as presents their preparation and characteristics, especially of some gossypol Schiff bases, through quantitative and structural analysis. The main attractive target sites of gossypol and its derivatives are Bcl-2 family proteins containing the anti-apoptosis proteins Bcl-2 and Bcl-XL. The molecular mechanism of gossypol analogs not only involves cell apoptosis but also autophagy, cell cycle arrest, and other abnormal cellular phenomena. Gossypol and its derivatives exert antitumor effects on different cancer types in vitro and in vivo, and demonstrate synergistic effects with other chemo- and radio- therapeutic treatments. In addition, several nanocarriers have been designed to load gossypol or its derivatives in order to expand the range of their applications and evaluate their combination effects with other anti-tumor agents. This review may serve as a reference for the rational application of gossypol analogs as anti-tumor agents.
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http://dx.doi.org/10.2174/0929867324666170523123655DOI Listing
July 2019

Thermostable polymeric nanomicelles of iridium(iii) complexes with aggregation-induced phosphorescence emission characteristics and their recyclable double-strand DNA monitoring.

J Mater Chem B 2017 Jan 1;5(1):123-133. Epub 2016 Dec 1.

Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, P. R. China.

Using 4,7-diphenyl-1,10-phenanthroline (DIP) as a main ligand and ethyl cyanoacrylate (ECA) as both an auxiliary ligand and a polymer skeleton, polymer iridium(iii) complexes (DIP)Ir(ECA) and their nanomicelles with aggregation-induced phosphorescence emission (AIPE) activity were synthesized. The morphology, chemical structure and composition of (DIP)Ir(ECA) nanomicelles were characterized using transmission electron microscopy, size distribution/zeta potential analysis, nuclear magnetic resonance, and Fourier transform infrared spectroscopy. Their AIPE-active effects and double-stranded DNA (dsDNA) monitoring abilities were determined using phosphorescence spectroscopy using a spectrophotofluorometer. The results showed that (DIP)Ir(ECA) nanomicelles had a good thermostability within 0-100 °C and their size distribution was 29.14 ± 1.46 nm. These nanomicelles showed AIPE-active effects and their phosphorescence intensity increased nearly 30-fold in water compared to in acetone. These nanomicelles could be used in AIPE-active intracellular imaging and dsDNA monitoring. Owing to the specific phosphorescence quenching that occurred when dsDNA encountered (DIP)Ir(ECA) nanomicelles, thermostable (DIP)Ir(ECA) nanomicelles could quickly detect dsDNA with high sensitivity and could be conveniently applied not only in monitoring DNA degradation in a wider pH range (specifically in an acidic environment), but also during PCR procedures. More importantly, both (DIP)Ir(ECA) nanomicelles and immobilized DNase I could be recycled and utilized at least four times using our novel phosphorescence "quenching-recovery" dsDNA detection procedure. The polymeric (DIP)Ir(ECA) nanomicelles were fast, sensitive, and convenient in monitoring dsDNA and could be recycled four times owing to their thermostability, indicating their great potential in biomedical and environmental applications.
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http://dx.doi.org/10.1039/c6tb02336cDOI Listing
January 2017
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