Publications by authors named "Jingjing Li"

1,052 Publications

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Genome sequencing sheds light on the contribution of structural variants to Brassica oleracea diversification.

BMC Biol 2021 May 5;19(1):93. Epub 2021 May 5.

National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, 100097, China.

Background: Brassica oleracea includes several morphologically diverse, economically important vegetable crops, such as the cauliflower and cabbage. However, genetic variants, especially large structural variants (SVs), that underlie the extreme morphological diversity of B. oleracea remain largely unexplored.

Results: Here we present high-quality chromosome-scale genome assemblies for two B. oleracea morphotypes, cauliflower and cabbage. Direct comparison of these two assemblies identifies ~ 120 K high-confidence SVs. Population analysis of 271 B. oleracea accessions using these SVs clearly separates different morphotypes, suggesting the association of SVs with B. oleracea intraspecific divergence. Genes affected by SVs selected between cauliflower and cabbage are enriched with functions related to response to stress and stimulus and meristem and flower development. Furthermore, genes affected by selected SVs and involved in the switch from vegetative to generative growth that defines curd initiation, inflorescence meristem proliferation for curd formation, maintenance and enlargement, are identified, providing insights into the regulatory network of curd development.

Conclusions: This study reveals the important roles of SVs in diversification of different morphotypes of B. oleracea, and the newly assembled genomes and the SVs provide rich resources for future research and breeding.
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http://dx.doi.org/10.1186/s12915-021-01031-2DOI Listing
May 2021

An Engineered Protein Adhesive with Properties of Tissue Integration and Controlled Release for Efficient Cartilage Repair.

Adv Healthc Mater 2021 May 5:e2100109. Epub 2021 May 5.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.

Cartilage damage is a prevalent health concern among humans. The inertness of cartilage, the absence of self-healing properties, and the lack of appropriate repair materials that integrate into the tissue pose a significant challenge for cartilage repair. Thus, it is important to develop novel soft biomaterials with strong tissue adhesion and chondrogenic capabilities for cartilage repair. Herein, a new type of protein adhesive is reported that exhibits superior cartilage repair performance. The material is fabricated by the electrostatic combination of chondroitin sulfate (CS) and positively charged elastin-like protein, which is derived from natural components of the extracellular matrix (ECM). The adhesive showed robust adhesion properties on different tissue substrates, offering a favorable environment for cartilage tissue integration. Noncovalent bonding between CS molecules in the glue allows for its controlled release, which is required for efficient chondrogenic differentiation. When implanted into a rat model of cartilage defect, this protein adhesive exhibited beneficial healing effects, as evidenced by enhanced chondrogenesis, sufficient ECM production, and lateral integration. Therefore, this engineered protein complex is a promising candidate for translational application in the field of cartilage repair.
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http://dx.doi.org/10.1002/adhm.202100109DOI Listing
May 2021

Development of an Antigen-Antibody Co-Display System for Detecting Interaction of G-Protein-Coupled Receptors and Single-Chain Variable Fragments.

Int J Mol Sci 2021 Apr 29;22(9). Epub 2021 Apr 29.

Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.

G-protein-coupled receptors (GPCRs), especially chemokine receptors, are ideal targets for monoclonal antibody drugs. Considering the special multi-pass transmembrane structure of GPCR, it is often a laborious job to obtain antibody information about off-targets and epitopes on antigens. To accelerate the process, a rapid and simple method needs to be developed. The split-ubiquitin-based yeast two hybrid system (YTH) was used as a blue script for a new method. By fusing with transmembrane peptides, scFv antibodies were designed to be anchored on the cytomembrane, where the GPCR was co-displayed as well. The coupled split-ubiquitin system transformed the scFv-GPCR interaction signal into the expression of reporter genes. By optimizing the topological structure of scFv fusion protein and key elements, including signal peptides, transmembrane peptides, and flexible linkers, a system named Antigen-Antibody Co-Display (AACD) was established, which rapidly detected the interactions between antibodies and their target GPCRs, CXCR4 and CXCR5, while also determining the off-target antibodies and antibody-associated epitopes. The AACD system can rapidly determine the association between GPCRs and their candidate antibodies and shorten the research period for off-target detection and epitope identification. This system should improve the process of GPCR antibody development and provide a new strategy for GPCRs antibody screening.
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http://dx.doi.org/10.3390/ijms22094711DOI Listing
April 2021

Adversarial Entropy Optimization for Unsupervised Domain Adaptation.

IEEE Trans Neural Netw Learn Syst 2021 May 3;PP. Epub 2021 May 3.

Domain adaptation is proposed to deal with the challenging problem where the probability distribution of the training source is different from the testing target. Recently, adversarial learning has become the dominating technique for domain adaptation. Usually, adversarial domain adaptation methods simultaneously train a feature learner and a domain discriminator to learn domain-invariant features. Accordingly, how to effectively train the domain-adversarial model to learn domain-invariant features becomes a challenge in the community. To this end, we propose in this article a novel domain adaptation scheme named adversarial entropy optimization (AEO) to address the challenge. Specifically, we minimize the entropy when samples are from the independent distributions of source domain or target domain to improve the discriminability of the model. At the same time, we maximize the entropy when features are from the combined distribution of source domain and target domain so that the domain discriminator can be confused and the transferability of representations can be promoted. This minimax regime is well matched with the core idea of adversarial learning, empowering our model with transferability as well as discriminability for domain adaptation tasks. Also, AEO is flexible and compatible with different deep networks and domain adaptation frameworks. Experiments on five data sets show that our method can achieve state-of-the-art performance across diverse domain adaptation tasks.
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http://dx.doi.org/10.1109/TNNLS.2021.3073119DOI Listing
May 2021

Application of methane and hydrogen-based breath test in the study of gestational diabetes mellitus and intestinal microbes.

Diabetes Res Clin Pract 2021 Apr 28:108818. Epub 2021 Apr 28.

Department of Obstetrics and Gynaecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan ,China.

Objective: Gestational diabetes mellitus (GDM) is a common complication of pregnancy. The purpose of this study was to compare the incidence of small intestinal bacterial overgrowth (SIBO) in patients with GDM and the control group by methane and hydrogen lactulose breath test (LBT), and to explore its relationship with inflammation, vitamins, and the outcomes of maternal and child.

Methods: LBT was detected in 220 GDM patients, 160 pregnancy control patients and 160 pre-pregnancy control patients. The fasting blood glucose, white blood cells, vitamin A, D, E, neonatal weight , neonatal blood glucose and so on were compared and analyzed.

Results: There was no statistical significance in the general data of the three groups. The proportion of abdominal distension in the GDM group was higher than that in the other two groups (P < 0.001). The positive rates of SIBO+ in GDM group, gestational control group and pre-pregnancy control group were 54.55%, 27.50% and 14.38%, respectively. The average abundance of hydrogen and methane in GDM group was significantly higher than that in control group at each time point. In the GDM group, SIBO+ subjects had higher levels of fasting blood glucose, glycoglycated hemoglobin, C-reactive protein, neonatal weight, and lower levels of vitamin D and neonatal blood glucose (P < 0.001).

Conclusion: Patients with GDM have a high incidence of SIBO, and SIBO may further increase their blood glucose by affecting inflammatory response and vitamin level, and even affect the outcome of mother and child.
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http://dx.doi.org/10.1016/j.diabres.2021.108818DOI Listing
April 2021

Sirolimus for the treatment of kaposiform hemangioendothelioma: In a trough level-dependent way.

J Dermatol 2021 May 1. Epub 2021 May 1.

Department of Pediatric Surgery, Shanghai Children's Medical Center Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

With the accumulation of clinical practice, sirolimus is now widely viewed as an effective agent in kaposiform hemangioendothelioma (KHE) treatment using a dose based on experience. Therefore, this retrospective research aimed to provide evidence-based suggestions on the most appropriate dose and trough level of sirolimus. All unresectable KHE cases diagnosed at our center from January 2016 to December 2019 were included. Sirolimus monotherapy was initiated when there was no sign of Kasabach-Merritt phenomenon (KMP) at a dose of 0.8 mg/m twice a day in order to keep the trough level at 5-20 ng/mL. Patients' clinical information, tumor volume change, trough level fluctuation, and complication occurrence were all recorded. Efficacy represented by tumor shrinkage speed and safety manifested by complication grades were compared between different trough level groups (5-10 vs. 10-15 vs. >15 ng/mL). Twenty-one patients (10 girls and 11 boys) were enrolled. There were eight patients in the 5-10 ng/mL group, seven in the 10-15 ng/mL group, and six in the more than 15 ng/mL group. Trough level over 10 ng/mL manifested better efficacy in tumor shrinkage (t-test, p = 0.011) while a level over 15 ng/mL had no further benefit in efficacy (t-test, p = 0.65). In addition, tumors at a central location reacted better to sirolimus (t-test, p = 0.022). No significant differences were observed in complication occurrence among different concentrations, although boys seemed to be at higher risk of more severe complications (>grade II, χ -test, p = 0.009, odds ratio = 4.52, range = 1.20-17.24). It proved to be most efficacious in the management of KHE at a trough level between 10 and 15 ng/mL. Such concentration was safe and well tolerated.
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http://dx.doi.org/10.1111/1346-8138.15905DOI Listing
May 2021

Oleic acid induces A7r5 cell proliferation and migration associated with increased expression of HGF and p‑p38.

Mol Med Rep 2021 Jul 28;24(1). Epub 2021 Apr 28.

Laboratory of Disorders Genes and Department of Pharmacology, Jishou University School of Pharmacy, Jishou, Hunan 416000, P.R. China.

The phenotypes and mechanisms underlying the proliferation and migration of vascular smooth muscle cells (VSMCs) induced by oleic acid (OA) are not completely understood. Therefore, the aim of the present study was to further elucidate the effects of OA on the proliferation and migration of VSMCs. Using A7r5 cells, the hepatocyte growth factor (HGF) inhibitor PHA665752 and the p38 MAPK inhibitor SB203580 were utilized, and Cell Counting Kit‑8 (CCK‑8) assays, Transwell assays, flow cytometry, ELISAs, western blotting and reverse transcription‑quantitative PCR (RT‑qPCR) were conducted to assess the effects of OA. CCK‑8 assays indicated that OA promoted (at 5 and 50 mol/l) or inhibited (at 800 mol/l) A7r5 cell proliferation in a time‑ and concentration‑dependent manner (P<0.05). Transwell assays revealed that OA also promoted (at 50 mol/l) or inhibited (at 800 mol/l) A7r5 cell migration (P<0.05). Moreover, cell‑cycle analysis identified that 50 mol/l OA reduced the cellular population in the G/G phase and enhanced the cellular population in the S phase (P<0.05), whereas 800 mol/l OA increased the cell number in the G/G phase and decreased the cell number in the S phase (P<0.05). In addition, OA promoted (at 50 mol/l) or inhibited (at 800 mol/l) the expression level of HGF in A7r5 cells, as demonstrated via ELISA, western blotting and RT‑qPCR analyses (P<0.05). It was also found that OA promoted (at 50 mol/l) or inhibited (at 800 mol/l) the expression level of phosphorylated (p)‑p38 in A7r5 cells, as indicated by western blotting (P<0.05). Furthermore, the cell proliferation, migration and HGF expression induced by OA (50 mol/l) were mitigated by treatment with PHA665752 (0.1 mol/l) (P<0.05), and the cell proliferation, migration and p‑p38 expression induced by OA (50 mol/l) were mitigated by SB203580 (2 mol/l) (P<0.05). Thus, the results suggested that OA served a role in the proliferation and migration of VSMCs via HGF and the p38 MAPK pathway. Moreover, the proliferation and migration of VSMCs induced by OA was associated with increased expression levels of HGF and p‑p38. Taken together, OA, HGF and p38 MAPK may be potential therapeutic targets for the treatment of atherosclerosis.
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http://dx.doi.org/10.3892/mmr.2021.12123DOI Listing
July 2021

Current status of ctDNA in precision oncology for hepatocellular carcinoma.

J Exp Clin Cancer Res 2021 Apr 26;40(1):140. Epub 2021 Apr 26.

Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Number 301, Middle Yanchang road, Jing'an, Shanghai, 200072, China.

The conventional method used to obtain a tumor biopsy for hepatocellular carcinoma (HCC) is invasive and does not evaluate dynamic cancer progression or assess tumor heterogeneity. It is thus imperative to create a novel non-invasive diagnostic technique for improvement in cancer screening, diagnosis, treatment selection, response assessment, and predicting prognosis for HCC. Circulating tumor DNA (ctDNA) is a non-invasive liquid biopsy method that reveals cancer-specific genetic and epigenetic aberrations. Owing to the development of technology in next-generation sequencing and PCR-based assays, the detection and quantification of ctDNA have greatly improved. In this publication, we provide an overview of current technologies used to detect ctDNA, the ctDNA markers utilized, and recent advances regarding the multiple clinical applications in the field of precision medicine for HCC.
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http://dx.doi.org/10.1186/s13046-021-01940-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074474PMC
April 2021

A novel compound heterozygous variant in leading to mild Schimke immune-osseous dysplasia identified using whole-exome sequencing.

J Int Med Res 2021 Apr;49(4):3000605211010644

Genetics and Prenatal Diagnosis Center, The First Affiliated Hospital of Zhengzhou University, Erqi District, 1 Jianshe East Road, Zhengzhou, P. R. China.

Schimke immuno-osseous dysplasia (SIOD) is a rare autosomal recessive inherited disorder that is caused by the mutation. The phenotype can vary from mild to severe on the basis of the patient's age at onset. Herein, we report the case of a 14-year-old Chinese boy who presented with short stature, focal segmental glomerulosclerosis (FSGS), and facial dysmorphism. Genetic analysis revealed two compound heterozygous missense mutations, including a well-known mutation (c.1933C>T, p.R645C) and a novel mutation (c.2479G>A, p.V827M) in the gene, which were inherited from his parents. analyses showed that the c.2479G>A (p.V827M) variant affects a highly conserved residue within the ATPase catalytic domain. Finally, we established the diagnosis of mild SIOD and treated the patient with diuretics and angiotensin receptor blockers. This report expands the mutational spectrum of and reinforces the importance of a detailed clinical evaluation, molecular detection, and appropriate genetic counseling.
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http://dx.doi.org/10.1177/03000605211010644DOI Listing
April 2021

Applications of the CRISPR-Cas system for infectious disease diagnostics.

Expert Rev Mol Diagn 2021 Apr 26. Epub 2021 Apr 26.

Jinming Yu Academician Workstation of Oncology, Weifang Medical College, Shandong, China.

Introduction: Rapid and accurate diagnostic approaches are essential for impeding the spread of infectious diseases. This review aims to summarize current progress of clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) systems in the applications for diagnostics of infectious diseases including the ongoing COVID-19 epidemic.

Areas Covered: In this review, we discuss class 2 CRISPR-Cas biosensing systems-based diagnostics in various emerging and re-emerging infectious diseases, CRISPR-Cas systems have created a new era for early diagnostics of infectious diseases, especially with the discovery of the collateral cleavage activity of Cas12 and Cas13. We mainly focus on different CRISPR-Cas effectors for the detection of pathogenic microorganisms as well as provide a detailed explanation of the pros and cons of CRISPR-Cas biosensing systems. In addition, we also introduce future research perspectives.

Expert Opinion: However, further improvement of newly discovered systems and engineering existing ones should be developed to increase the specificity, sensitivity or stability of the diagnostic tools. It may be a long journey to finish the clinical transition from research use. CRISPR-Cas approaches will emerge as more promising and robust tools for infectious disease diagnosis in the future.
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http://dx.doi.org/10.1080/14737159.2021.1922080DOI Listing
April 2021

Age-specific risk factors of severe pneumonia among pediatric patients hospitalized with community-acquired pneumonia.

Ital J Pediatr 2021 Apr 23;47(1):100. Epub 2021 Apr 23.

Department of Pediatrics, Fujian Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, China.

Background: Risk factors that predispose the development of severe community-acquired pneumonia (CAP) among pediatric CAP patients of different age ranges are yet to be identified.

Methods: We retrospectively analyzed pediatric in-patients (< 6 years old) diagnosed with CAP in our hospital. We subdivided patients into four age groups (< 6 months, 6 months-1 year, 1-2 years, and 2-6 years). Their medical records, including demographic information, clinical features, laboratory findings, and chest radiographic reports, were reviewed and collected for further analysis. Univariate logistic regression analysis and stepwise regression analysis were applied to identify risk factors associated with severe CAP and ICU admission for overall patients and age-stratified subgroups.

Results: A total of 20,174 cases were initially included. Among them, 3309 (16.40%) cases were identified as severe CAP, and 2824 (14.00%) cases required ICU admission. Potential risk factors for severe CAP and ICU admission identified by univariate analysis included younger age, rural residency, premature birth, low birth weight (LBW), formula feeding, congenital heart disease (CHD), history of pneumonia or neonatal jaundice, patients with other health issues, certain symptoms (manifesting wheezing, dyspnea, cyanosis, but have no cough or fever), abnormal laboratory findings (abnormal levels of white blood cells, albumin, and C-reactive protein and RSV infection), and chest X-ray (odds ratio [OR] > 1 for all). CHD, low albumin, proteinuria, abnormal chest x-ray were independent risks factors across different age groups, whereas birth or feeding history, history of pneumonia, cyanosis or dyspnea on admission, and RSV infection were independent risk factors for only younger kids (< 1 year), and wheezing was an independent risk factor only for older children (2-5 years old).

Conclusions: Risk factors predicting disease severity among children hospitalized with CAP vary with age. Risk factor stratification of pediatric CAP based on age-specific risk factors can better guide clinical practice.

Trial Registration: This study has been registered in China, with the registration number being ChiCTR2000033019 .
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http://dx.doi.org/10.1186/s13052-021-01042-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062938PMC
April 2021

Concomitant mutation status of -rearranged non-small cell lung cancers and its prognostic impact on patients treated with crizotinib.

Transl Lung Cancer Res 2021 Mar;10(3):1525-1535

Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin Lung Cancer Center, Tianjin, China.

Background: In non-small cell lung cancer (NSCLC), anaplastic lymphoma kinase () rearrangement characterizes a subgroup of patients who show sensitivity to tyrosine kinase inhibitors (TKIs). However, the prognoses of these patients are heterogeneous. A better understanding of the genomic alterations occurring in these tumors could explain the prognostic heterogeneity observed in these patients.

Methods: We retrospectively analyzed 96 patients with NSCLC with detected by immunohistochemical staining (VENTANA anti-(D5F3) Rabbit Monoclonal Primary Antibody). Cancer tissues were subjected to next-generation sequencing using a panel of 520 cancer-related genes. The genomic landscape, distribution of fusion variants, and clinicopathological characteristics of the patients were evaluated. The correlations of genomic alterations with clinical outcomes were also assessed.

Results: Among the 96 patients with immunohistochemically identified fusions, 80 (83%) were confirmed by next-generation sequencing. mutation was the most commonly co-occurring mutation with rearrangement. Concomitant driver mutations [2 Kirsten rat sarcoma viral oncogene homolog () G12, 1 epidermal growth factor receptor () 19del, and 1 exon 14 skipping] were also observed in 4 adenocarcinomas. Echinoderm microtubule associated protein-like 4 ()- fusions were identified in 95% of -rearranged patients, with 16.2% of them also harboring additional non-- fusions. Nineteen non- translocation partners were also discovered, including 10 novel ones. Survival analyses revealed that patients concurrently harboring alterations showed a trend toward shorter progression-free survival (6 13 months, P=0.064) and significantly shorter overall survival (11 32 months, P=0.004) than did -wild-type patients. Patients with concomitant alterations in the signaling pathway also had a shorter median overall survival than those without such alterations (23 32 months, P=0.014), whereas progression-free survival did not differ significantly.

Conclusions: The spectrum of -fusion variants and the landscape of concomitant genomic alterations were delineated in 96 NSCLC patients. Our study also demonstrated the prognostic value of concomitant alterations in crizotinib-treated patients, which could facilitate improved stratification of -rearranged NSCLC patients in the selection of candidates who could optimally benefit from therapy.
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http://dx.doi.org/10.21037/tlcr-21-160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044492PMC
March 2021

Deep sequencing reveals the genomic characteristics of lung adenocarcinoma presenting as ground-glass nodules (GGNs).

Transl Lung Cancer Res 2021 Mar;10(3):1239-1255

Key Laboratory of Genomics and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.

Background: The concept of multi-step progression from atypical adenomatous hyperplasia (AAH) to invasive adenocarcinoma (ADC) has been proposed, and ground-glass nodules (GGNs) may play a critical role during the early lung tumorigenesis. We present the first comprehensive description of the genomic architecture of GGNs to unravel the genetic basis of GGN.

Methods: We investigated 30 GGN-like lungs ADC by performing >1,000× whole-exome sequencing (WES) and characterized the genomic variations and evaluate the relationship between the clinicopathologic and molecular characteristics in this disease.

Results: Despite the low somatic mutation burden, GGNs exhibited high intratumor heterogeneity (ITH) characterized by the proportion of subclonal mutations. Different mutagenesis shaped the genomes of GGN during cancer evolution and were mostly featured by molecular clock-like signatures that occur in clonal mutations and defective DNA mismatch signatures that occur in subclonal mutations. Moreover, 10.7-67.1% clonal mutations occurred after whole-genome doubling (WGD), indicating that WGD could be a frequent truncal event in GGNs. Samples with WGD showed higher genomic instability but lower ITH. These GGNs were characterized by recurrent focal copy-number changes that are highly associated with tumorigenesis, with only two genes ( and ) that were recurrently mutated. Additionally, GGNs with different pathological subtypes or computed tomography (CT) features exhibited distinct genetic characteristics. Lepidic predominant or pure GGNs in CT images carried a lower mutation burden and had a relatively stable genome than nonlepidic or mixed GGNs. GGNs with mutations tended to accompany a pathologically lepidic pattern, indicating may drive the distinct subtype of lung cancer with better prognosis.

Conclusions: These findings facilitated interpreting the genomic characteristics of GGNs, provided insight into the early stages of lung cancer evolution, and possessed potential clinical significance.
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http://dx.doi.org/10.21037/tlcr-20-1086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044491PMC
March 2021

Paeoniflorin protects against dextran sulfate sodium (DSS)-induced colitis in mice through inhibition of inflammation and eosinophil infiltration.

Int Immunopharmacol 2021 Apr 19;97:107667. Epub 2021 Apr 19.

Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University, China; State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, China; Shenzhen Research Institute of Nanjing University, Shenzhen, China. Electronic address:

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) that causes inflammation and ulcers in the digestive tract. The treatment commonly includes anti-inflammatory agents like 5-aminosalicylic acid or corticosteroids or biologics for people with UC who are no longer responding to corticosteroids. The radices of Paeonia lactiflora Pall. or similar plants of the Paeonia genus have been used in Chinese medicine to treat certain diseases that resemble the symptoms of UC. Paeoniflorin, a terpenoid glycoside, is a major active component for the anti-inflammatory and antitumor activity. In this study, we evaluated the therapeutic effect of paeoniflorin (PF) against dextran sulfate sodium (DSS)-induced colitis in mice and found that PF exhibited protective activity against colitis. PF treatment suppressed NF-κB pathway activation, resulting down regulation of pro-inflammatory factor expression. In addition, we detected reduction in eosinophil-related chemokine gene expression and eosinophil infiltration. The treatment also reversed Treg cell population suppression. Although PF treatment did not block COX2 induction, the compound weakly inhibited COX2 activity in an enzymatic assay. Taken together, PF exerts its therapeutic activity against UC through inhibition of inflammation and eosinophil infiltration.
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http://dx.doi.org/10.1016/j.intimp.2021.107667DOI Listing
April 2021

Genetically Engineered Polypeptide Adhesive Coacervates for Surgical Applications.

Angew Chem Int Ed Engl 2021 Apr 22. Epub 2021 Apr 22.

Tsinghua University, Department of Chemistry, qinghua yuan, 100084, Beijing, CHINA.

Adhesive hydrogels have been developed for wound healing applications. However, their adhesive performance is impaired dramatically due to their high swelling on wet tissues. To tackle this challenge, we fabricated a new type of non-swelling protein adhesive for underwater and in vivo applications. In this soft material, the electrostatic complexation between supercharged polypeptides with oppositely charged surfactants containing 3,4-dihydroxylphenylalanine or azobenzene moieties plays an important role for the formation of ultra-strong adhesive coacervates. Remarkably, the adhesion capability is superior to commercial cyanoacrylate when tested in ambient conditions. Moreover, the adhesion is stronger than other reported protein-based adhesives in underwater environment. The ex vivo and in vivo experiments demonstrate the persistent adhesive performance and outstanding behaviors for wound sealing and healing. Thus, this new type of genetically engineered adhesive coacervates is a very promising alternative for surgical applications.
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http://dx.doi.org/10.1002/anie.202100064DOI Listing
April 2021

Prediction of repeated-dose intravenous ketamine response in major depressive disorder using the GWAS-based machine learning approach.

J Psychiatr Res 2021 Apr 12;138:284-290. Epub 2021 Apr 12.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Major depressive disorder (MDD) is one of the most common psychiatric disorders. Various clinical studies have shown that the N-methyl-D-aspartate (NMDA) receptor antagonist ketamine has rapid, robust, and sustained antidepressant effects. However, given the concerns about the adverse effects of ketamine on patients, it would be important to identify a set of biomarkers that could be used to predict clinical outcomes for its treatment. A total of 83 MDD patients received treatment with six ketamine infusions for up to 2 weeks and were classified into "responders" or "non-responders" based on an average change in the HAMD score >50% from baseline. A nested cross-validation approach was applied to prevent information leakage and overestimation of model performance. The initial dataset was divided randomly into training and test sets in a nested six-fold cross-validation. We first performed genome-wide logistic regression to find potentially significant variants related to treatment response and then selected the top SNPs based on the genetic association results using the random forests algorithm. Subsequently, six machine learning models were employed to construct prediction models by using ten-fold cross-validation. A series of model comparisons showed that the best performing fold was characterized by accuracy of 0.85, precision of 0.75, and a sensitivity of 1.00 with the support vector machine algorithm. Together, these findings demonstrated that the machine learning approach can predict the treatment outcomes of multiple ketamine infusions on the basis of the genotyping information of each participant.
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http://dx.doi.org/10.1016/j.jpsychires.2021.04.014DOI Listing
April 2021

Dermoscopic monitoring of erythroplasia of Queyrat treated with photodynamic therapy.

Indian J Dermatol Venereol Leprol 2021 May-Jun;87(3):396-399

Department of Dermatologic Surgery, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu Province, China.

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http://dx.doi.org/10.25259/IJDVL_89_20DOI Listing
January 2020

Polyene-Producing spp. From the Fungus-Growing Termite Exhibit High Inhibitory Activity Against the Antagonistic Fungus .

Front Microbiol 2021 1;12:649962. Epub 2021 Apr 1.

State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, China.

Fungus-growing termites are engaged in a tripartite mutualism with intestinal microbes and a monocultivar ( sp.) in the fungus garden. The termites are often plagued by entomopathogen () and fungus garden is always threatened by competitors ( spp.). Here, we aim to understand the defensive role of intestinal microbes, the actinomycetes which were isolated from the gut of . We obtained 44 antifungal isolates, which showed moderate to strong inhibition to sp. HPLC analysis indicated that different types of polyenes (tetraene, pentene, and heptaene) existed in the metabolites of 10 strong antifungal strains. Two pentene macrolides (pentamycin and 1'14-dihydroxyisochainin) were firstly purified from strain HF10, both exhibiting higher activity against sp. and than cultivar . Subsequently, tetraene and heptaene related gene disruption assay showed that the mutant strains lost the ability to produce corresponding polyenes, and they also had significantly decreased activities against sp. and compared to that of wild type strains. These results indicate that polyene-producing from the guts of have strong inhibition to competitor fungus and polyenes contribute to inhibitory effects on sp.
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http://dx.doi.org/10.3389/fmicb.2021.649962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047067PMC
April 2021

Tumor-targeted Gd-doped mesoporous FeO nanoparticles for T/T MR imaging guided synergistic cancer therapy.

Drug Deliv 2021 Dec;28(1):787-799

School of Medical Imaging, Xuzhou Medical University, Xuzhou, China.

In this study, a novel intelligent nanoplatform to integrate multiple imaging and therapeutic functions for targeted cancer theranostics. The nanoplatform, DOX@Gd-MFeO NPs, was constructed Gd-doped mesoporous FeO nanoparticles following with the doxorubicin (DOX) loading in the mesopores of the NPs. The DOX@Gd-MFeO NPs exhibited good properties in colloidal dispersity, photothermal conversion, NIR triggered drug release, and high T/T relaxicity rate (=9.64 mMs, = 177.71 mMs). Benefiting from the high MR contrast, DOX@Gd-MFeO NPs enabled simultaneous T/T dual-modal MR imagining on 4T1 bearing mice and the MR contrast effect was further strengthened by external magnetic field. In addition, the DOX@Gd-MFeO NPs revealed the strongest inhibition to the growth of 4T1 and under NIR irradiation and guidance of external magnetic field. Moreover, biosafety was also validated by and tests. Thus, the prepared DOX@Gd-MFeO NPs would provide a promising intelligent nanoplatform for dual-modal MR imagining guided synergistic therapy in cancer theranostics.
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http://dx.doi.org/10.1080/10717544.2021.1909177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079076PMC
December 2021

Reducing bias to source samples for unsupervised domain adaptation.

Neural Netw 2021 Mar 26;141:61-71. Epub 2021 Mar 26.

School of Computer Science and Engineering, University of Electronic Science and Technology of China, China.

Unsupervised Domain Adaptation (UDA) makes predictions for the target domain data while labels are only available in the source domain. Lots of works in UDA focus on finding a common representation of the two domains via domain alignment, assuming that a classifier trained in the source domain can be generalized well to the target domain. Thus, most existing UDA methods only consider minimizing the domain discrepancy without enforcing any constraint on the classifier. However, due to the uniqueness of each domain, it is difficult to achieve a perfect common representation, especially when there is low similarity between the source domain and the target domain. As a consequence, the classifier is biased to the source domain features and makes incorrect predictions on the target domain. To address this issue, we propose a novel approach named reducing bias to source samples for unsupervised domain adaptation (RBDA) by jointly matching the distribution of the two domains and reducing the classifier's bias to source samples. Specifically, RBDA first conditions the adversarial networks with the cross-covariance of learned features and classifier predictions to match the distribution of two domains. Then to reduce the classifier's bias to source samples, RBDA is designed with three effective mechanisms: a mean teacher model to guide the training of the original model, a regularization term to regularize the model and an improved cross-entropy loss for better supervised information learning. Comprehensive experiments on several open benchmarks demonstrate that RBDA achieves state-of-the-art results, which show its effectiveness for unsupervised domain adaptation scenarios.
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http://dx.doi.org/10.1016/j.neunet.2021.03.021DOI Listing
March 2021

Engineered Protein Photo-Thermal Hydrogels for Outstanding In Situ Tongue Cancer Therapy.

Adv Mater 2021 Apr 16:e2100619. Epub 2021 Apr 16.

Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, China.

Surgical excision is the main choice for tongue cancer treatment. However, the physiological functions of oral and maxillofacial regions might be severely impaired and high risk of tongue tumor recurrence cannot be avoided. It is thus becoming urgently important to develop alternative strategies for tongue cancer therapy. In this regard, a new class of near-infrared (NIR) light-responsive and peritumoral injectable hydrogel is fabricated with extraordinary photothermal therapy (PTT) for in situ tongue tumors. The as-prepared soft material exhibits good biocompatibility and ultra-strong photothermal effect due to the formed network by negatively charged proteins, chitosan molecules, and Ag AuS nanoparticles (NPs). In a well-constructed in situ tongue tumor model, tumors can be efficiently eradicated by one-time PTT treatment. Importantly, there are no side effects on surrounding normal tissues and potential tumor recurrence is inhibited. In stark contrast to traditional surgical excision, such biomaterials hold great potential for clinical treatment of oral cancers.
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http://dx.doi.org/10.1002/adma.202100619DOI Listing
April 2021

A Novel Autoantibody Induced by Bacterial Biofilm Conserved Components Aggravates Lupus Nephritis.

Front Immunol 2021 25;12:656090. Epub 2021 Mar 25.

Beijing Institute of Pharmacology and Toxicology, Beijing, China.

Systemic lupus erythematosus (SLE) is a systemic autoimmune disease with multiple autoantibody production and often affects the kidneys, known as lupus nephritis. However, the mechanism underlying lupus nephritis development is unclear. Biofilms that protect bacteria from stress are ubiquitous in almost every environment. Here, we identified that a conserved peptide (HU1) derived from DNABII proteins, one of major bacterial biofilm components, was specifically recognized by sera from about 47% patients with SLE. Moreover, the serum anti-HU1 levels showed a significant positive correlation with lupus nephritis occurrence. Presence of antibodies against HU1 in pristane-induced mice aggravated lupus nephritis, although these antibodies also attenuated bacterial biofilm formation. We further identified that antibodies against HU1 cross-recognized protein disulfide isomerase (P4HB) located on the renal cell surface and inhibited the activities of this enzyme. Our findings reveal a novel mechanism underlying the development of lupus nephritis triggered by bacterial biofilms.
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http://dx.doi.org/10.3389/fimmu.2021.656090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027312PMC
March 2021

Systemic Inflammatory Markers of Resectable Colorectal Cancer Patients with Different Mismatch Repair Gene Status.

Cancer Manag Res 2021 30;13:2925-2935. Epub 2021 Mar 30.

Department of Hepato-Pancreato-Biliary & Gastric Medical Oncology, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Cancer and Basic Medicine (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang Province, 310022, People's Republic of China.

Background: We aimed to assess the differences in gene expression and systemic inflammatory markers in colorectal cancer (CRC) patients with different mismatch repair (MMR) statuses.

Methods: Bioinformatics analysis was used to identify the different expression genes in patients with CRC at different MMR statuses. A total of 208 patients with resectable colorectal cancer, including 104 deficient mismatch repair (dMMR) patients and 104 matched proficient mismatch repair (pMMR) patients, were retrospectively analyzed.

Results: Bioinformatics analysis showed that chemokine-mediated signaling pathway and inflammatory responses were the main differences in gene expression between dMMR and pMMR CRC patients. In all 208 patients with CRC, those with dMMR frequently had it located on the right side, with more mucinous adenocarcinoma and grade 3 tumors. Patients with dMMR had an earlier American Joint Committee on Cancer (AJCC) stage than pMMR patients. Meanwhile, lymph nodes (LNs) metastasis was more frequently negative in dMMR patients than pMMR patients. Interestingly, patients with CRC with dMMR had more regional lymph nodes removed during surgery, although with less metastatic cancer. Patients with resectable CRC with dMMR were more likely to have higher levels of neutrophil, monocyte, platelet, neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), C-reactive protein to albumin ratio (CAR), Glasgow prognostic score (GPS) and C-reactive protein (CRP). In patients with dMMR, those with higher levels of PLR, MLR, CAR, and co-effect present had shorter overall survival (OS) significantly. It was noteworthy that the prognosis of high levels of systemic inflammatory markers did not predict prolonged OS in patients with pMMR CRC.

Conclusion: dMMR CRC has presented a comprehensively distinct systemic inflammatory microenvironment. The systemic inflammatory response can predict oncological outcomes in patients with CRC with dMMR.
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http://dx.doi.org/10.2147/CMAR.S298885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019618PMC
March 2021

Comparison of formula-based PICC catheterisation versus common method for the treatment of newborns.

Int J Clin Pract 2021 Apr 5:e14210. Epub 2021 Apr 5.

Neonatal Department, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Aims: Whether PICC in newborns can improve its success rate and reduce the complications by using the formula method to calculate the depth of catheterisation.

Methods: A total of 130 newborns were prospectively studied from December 2018 to December 2019. All newborns were randomly divided into two groups that use formula method and common method respectively. The PICC catheter length of the two groups was observed. The unplanned extubation rate, one-time puncture success rate, catheter indwelling time, complications, duration of hospital stays and pain scores were recorded during the whole research process and compared between these two groups.

Results: The formula method significantly improved the puncture success rate and reduced the secondary adjustment rate. The length of the catheter adjustment was significantly lower than the control group. The retention time also increased significantly. The incidence of PICC-related complications, including phlebitis, thrombosis, catheter-related infection and catheter displacement, was significantly reduced by using the formula method.

Conclusions: The formula method in neonatal PICC catheter had great outcomes in terms of improving the success rate of one-time puncture and prolonging the indwelling time of catheter.
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http://dx.doi.org/10.1111/ijcp.14210DOI Listing
April 2021

Effectiveness and safety of low-level laser therapy in diabetic peripheral neuropathy: a protocol for a systematic review and meta-analysis.

Syst Rev 2021 Apr 2;10(1):96. Epub 2021 Apr 2.

School of Nursing, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, China.

Background: Diabetic peripheral neuropathy (DPN) is one of the most common chronic complications of diabetes mellitus. The main symptoms of DPN include numbness or pain in both extremities and paresthesia (such as formication or burning sensations), which greatly affect patients' quality of life. Pharmacological treatments for DPN are associated with both uncertain therapeutic effects and adverse effects, as well as with high costs. Some clinical studies have reported that low-level laser therapy (LLLT) relieves clinical symptoms and improves nerve function in patients with DPN. We intend to conduct a systematic review and meta-analysis to further evaluate the effectiveness and safety of LLLT for DPN.

Methods: The following electronic databases will be searched to retrieve literature from their inception until December 2020: MEDLINE (PubMed), Embase, Cochrane Central Register of Controlled Trials, Web of Science (the Science and Social Science Citation Index), CNKI, VIP, WanFang, and SinoMed. Simultaneously, clinical registration tests and gray literature will also be retrieved. Randomized controlled trials (RCTs) comparing LLLT with either sham LLLT, no (specific) treatment, or active conventional medical treatments will be included. The primary outcomes will be nerve conduction velocity as well as clinical scores that assess neurological function and related symptoms. The risk of bias of each study and quality of evidence will be assessed using the updated Cochrane Risk of Bias 2.0 tool and GRADE approach, respectively. A meta-analysis will then be conducted using Review Manager software version 5.3.

Discussion: This study will integrate RCTs and analyze data to provide a detailed summary of the evidence relating to the effects and safety of LLLT in patients with DPN. LLLT will be compared with sham LLLT, no (specific) treatment, or active conventional medical treatments, especially in terms of neurological function, quality of life, and adverse events. In conclusion, this systematic review will generate evidence regarding the use of LLLT to treat DPN, in terms of both its efficacy and safety.

Systematic Review Registration: This protocol was registered with the International Prospective Register of Systematic Reviews on April 2020 (registration number: CRD42020170625 ).
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http://dx.doi.org/10.1186/s13643-021-01656-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017787PMC
April 2021

Parent-Adolescent Communication, School Engagement, and Internet Addiction among Chinese Adolescents: The Moderating Effect of Rejection Sensitivity.

Int J Environ Res Public Health 2021 03 29;18(7). Epub 2021 Mar 29.

Center for Studies of Psychological Application, School of Psychology, South China Normal University, Guangzhou 510631, China.

Although a large body of research has indicated that parent-adolescent communication is a crucial protective factor for adolescent Internet addiction, the mediating and moderating mechanisms underlying this relationship remain unclear. To address this research gap, this study, based on social control theory and the organism-environment interaction model, was designed to test whether school engagement mediated the relationship between parent-adolescent communication and adolescent Internet addiction and whether this mediating effect was moderated by rejection sensitivity. A sample of 1006 adolescents ( = 13.16 years, = 0.67) anonymously completed the questionnaires. The results showed that the positive association between parent-adolescent communication and adolescent Internet addiction was mediated by school engagement. Moreover, this indirect link was stronger among adolescents with high rejection sensitivity than those with low rejection sensitivity. These findings highlighted school engagement as a potential mechanism linking parent-adolescent communication to adolescent Internet addiction, with high rejection sensitivity being an important risk factor amplifying this indirect effect. Intervention programs aimed at reducing Internet addiction among adolescents might benefit from the current research.
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http://dx.doi.org/10.3390/ijerph18073542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037552PMC
March 2021

Insight into the effect of manganese substitution on mesoporous hollow spinel cobalt oxides for catalytic oxidation of toluene.

J Colloid Interface Sci 2021 Jul 19;594:713-726. Epub 2021 Mar 19.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; National Engineering Laboratory for VOCs Pollution Control Technology and Equipment, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China; Guangdong Provincial Engineering and Technology Research Centre for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China. Electronic address:

The cobalt oxides and manganese oxides have high-activity potential for catalytic oxidation of volatile organic compounds (VOCs), while the mesoporous hollow morphology is crucial to the mass transfer of reactant and product. Therefore, it is worth investigating the effect of manganese substitution in mesoporous hollow cobalt oxides on catalytic oxidation. Herein, a partially disordered spinel structure is formed by the Mn substitution in CoO and the mesoporous hollow microsphere is improved in morphology homogeneity with the decrease of Co/Mn ratio in the range of 1.8-28.8. The 5Co1Mn (Mn-substituted CoO with Co/Mn at 5.4) exhibits outstanding catalytic activity for toluene oxidation with 50% CO generation at 237 °C, which is 21 °C lower than CoO. Moreover, the 5Co1Mn displays satisfactory stability in reusability, lifetime, and water resistance. The small defective crystallite, mesoporous hollow morphology, and high specific surface area endow Mn-substituted CoO with more surface chemical adsorbed oxygen, enhancing the catalytic oxidation of toluene. Theoretical calculation on (311) plane of CoO reveals that Mn or Mn substitution increases the formation energy of oxygen vacancy and makes it difficult to adsorb gaseous oxygen on the defective surface. The interaction between Co and Mn impedes the improvement of toluene oxidation because the mobility of lattice oxygen, the surface distribution of Co, and the ratio of surface adsorbed oxygen to surface lattice oxygen are hindered by Mn substitution. The chemical adsorbed oxygen is more active than lattice oxygen in the oxidation of adsorbed intermediates (phenolate, benzoate species, etc.). The Langmuir-Hinshelwood mechanism dominates in the catalytic oxidation at 200-250 °C, while the catalytic oxidation follows both the Langmuir-Hinshelwood mechanism and Mars-van Krevelen mechanism above 250 °C. This work provides some enlightenment for exploring the role of surface oxygen species in VOCs oxidation and uncovering the interaction in binary spinel oxides.
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http://dx.doi.org/10.1016/j.jcis.2021.03.093DOI Listing
July 2021

Prevalence and healthcare utilization of rare neurological diseases in Hong Kong: 2014-2018.

Eur J Neurol 2021 Apr 1. Epub 2021 Apr 1.

Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong SAR, China.

Background: There has been increasing attention focused on the epidemiology of rare diseases (RDs) in recent years. Rare neurological diseases (RNDs) constitute a significant proportion of RDs; however, relevant research is still lacking.

Methods: A list of ICD-10 codes corresponding to RNDs was compiled using adaptations from the Orphanet Classification of Rare Diseases, and classified into rare epilepsy, movement-related, neurocutaneous, neuroimmune, neurometabolic and neurodegenerative, neuromuscular and other RNDs. Using the Clinical Data Analysis and Reporting System, which holds public hospital healthcare records of Hong Kong anonymously, we calculated the prevalence and healthcare utilization of RND patients between 2014 and 2018. The list of RNDs was also used to review relevant pharmacological trials within the International Clinical Trials Registry Platform between 2009 and 2018.

Results: The prevalence of RNDs in Hong Kong is 3.6 in 1,000 individuals. Patients with RNDs had frequent emergency department, outpatient and inpatient healthcare utilization. The average annual cost per patient is estimated at HKD 182,075 (€ 19,688). Different categories of RNDs showed different patterns of healthcare utilization. Moreover, there were only 677 RND-related pharmacological trials during the study period, and no trial was found for 78% of RNDs.

Conclusions: This is one of the first population studies on the prevalence and healthcare utilization patterns of RNDs, with comprehensive reviews of RND-related pharmacological research. It shows high healthcare utilization rates among patients with RNDs, as well as a wide research gap in many RNDs. We call for better attention and tailored healthcare for these patients.
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http://dx.doi.org/10.1111/ene.14852DOI Listing
April 2021

A serotonin voltammetric biosensor composed of carbon nanocomposites and DNA aptamer.

Mikrochim Acta 2021 Apr 1;188(4):146. Epub 2021 Apr 1.

Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu-city, 41566, Republic of Korea.

A sensitive and selective voltammetric biosensor composed of layer-by-layer (LbL) self-assembly of positively charged poly(diallyldimethylammonium)-wrapped oxidized single-walled carbon nanotubes (PDDA-oSWCNTs), negatively charged serotonin (5-hydroxytryptamine, 5-HT)-specific aptamer, and tyrosinase on Au nanoparticles deposited screen printed carbon electrode was developed for measurement of 5-HT. Surface characteristics of 5-HT biosensor were explored using scanning electron microscopy, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. The respective effects of 5-HT-specific aptamer and oSWCNTs on the detection of 5-HT were investigated by differential pulse voltammetry (DPV). The peak current at the potential of 0.29 V (vs. Ag/AgCl) increased with respect to 5-HT concentration resulting in two dynamic ranges from 0.05 to 0.5 and 1 to 20 μM with a limit of detection of 2 nM from the LbL biosensor in buffer solution, which were better than those without the LbL of aptamer and oSWCNTs. The developed biosensor was applied to the direct determination of 5-HT concentrations in undiluted healthy control and Internet gaming disorder serum samples. The results were verified by comparison with those from liquid chromatography-mass spectrometric analyses.
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http://dx.doi.org/10.1007/s00604-021-04798-xDOI Listing
April 2021

Utility of photodynamic diagnosis plus reflectance confocal microscopy in detecting the margins of extramammary Paget disease.

Indian J Dermatol Venereol Leprol 2021 Mar-Apr;87(2):207-213

Department of Dermatologic Surgery, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing.

Background: Due to the clinically poorly delineated unclear margin of extramammary Paget disease, the recurrence rate after surgical resection is high.

Aims: To compare photodynamic diagnosis and photodynamic plus reflectance confocal microscopy diagnosis in determining the tumor margins in patients with extramammary Paget disease.

Methods: Thirty-six patients with histopathologically confirmed primary extramammary Paget disease between January 2017 to June 2018 were included in the study. The skin lesion margins were preoperatively observed by the naked eye and with photodynamic diagnosis and photodynamic diagnosis plus reflectance confocal microscopy and they were compared to the postoperative histopathological examination results.

Results: Among the 130 sections taken from 36 patients, 83 sections (63.8%, 83/130) had tumor margins beyond the macroscopic line with a distance of 3.5 ± 3.1mm and a median of 2.7mm. Forty-six sections (35.4%, 46/130) exceeded the photodynamic diagnosis marker line with a distance of 2.1 ± 1.7mm and a median of 1.5mm. Twenty seven sections (20.8%, 27/130) were obtained beyond the photodynamic diagnosis plus reflectance confocal microscopy marker line with a distance of 1.4 ± 1.2mm and a median of 0.9mm.

Limitations: Photodynamic diagnosis and reflectance confocal microscopy detection can be used to observe only the superficial margin of the tumor and not the deep part. Moreover, reflectance confocal microscopy was not used alone as a control.

Conclusion: In terms of determining the extramammary Paget disease margin invasively, photodynamic diagnosis and photodynamic diagnosis plus reflectance confocal microscopy were found superior to observations made with the naked eye, while photodynamic diagnosis plus reflectance confocal microscopy was superior to photodynamic diagnosis alone.
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http://dx.doi.org/10.25259/IJDVL_90_20DOI Listing
January 2020