Publications by authors named "Jingfeng Zhang"

61 Publications

Report of Eleven Patients of Subcutaneous Panniculitis-Like T-Cell Lymphoma: Clinicopathologic Features, F-FDG PET/CT Findings and Outcome.

Front Oncol 2021 1;11:650822. Epub 2021 Jul 1.

Nanfang PET Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Objectives: Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) is a fairly rare subtype of primary cutaneous lymphoma. This study aims to investigate the clinicopathologic features, F-FDG PET/CT findings, and outcome of patients with SPTCL.

Methods: A retrospective single-center study enrolled 11 patients with SPTCL between August 2010 and March 2020. A total of 26 F-FDG PET/CT scans were performed, and the initial and follow-up PET/CT imaging features, clinicopathologic and immunohistochemical characteristics, and outcome were analyzed.

Results: The male-to-female ratio was 1.2. The mean age at diagnosis was 24.2 years (age range: 13-48 years). Histopathological examinations revealed atypical T-lymphocyte rimming of individual subcutaneous adipocytes, mostly with CD2, CD3, CD4, CD5, CD8, CD56, T-cell intracellular antigen-1, Granzyme B, and high Ki-67 index. Multiple large skin ulcerations with a maximum diameter of 10 cm were observed in one of the 11 patients (9.1%, 1/11), and hemophagocytic syndrome was found in another one. At initial PET/CT scans, the lesions in all 11 patients showed increased uptake of F-FDG with a wide range of maximum standard uptake value (SUVmax) from 2.0 to 14.9. The morphology of the lesions presented as multiple nodules and/or disseminated plaques mainly involving the trunk and/or limbs. Five patients had extracutaneous non-lymph node lesions with SUVmax of 5.6 ± 2.8 on F-FDG PET/CT. No significant correlation between SUVmax and Ki-67 index was observed (r = 0.19, > 0.05). Follow-up F-FDG PET/CT scans in six patients showed complete remission of the disease in two, partial remission in three, and progressive disease in one. During the follow-up period, there was no death except for the patient with multiple ulcerations who died 4 months after diagnosis of SPTCL.

Conclusions: SPTCL may be a group of heterogeneous diseases with varying degrees of F-FDG uptake. F-FDG PET/CT demonstrates its usefulness in detecting disease extent, providing diagnostic work-up, staging, and evaluating treatment response of SPTCL. Multiple large skin ulcerations may be a factor of poor prognosis for patients with SPTCL.
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http://dx.doi.org/10.3389/fonc.2021.650822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281960PMC
July 2021

Prognostic Value of Pretreatment Overweight/Obesity and Adipose Tissue Distribution in Resectable Gastric Cancer: A Retrospective Cohort Study.

Front Oncol 2021 24;11:680190. Epub 2021 Jun 24.

Department of Thoracic Surgery, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China.

Background: This is a study aimed at exploring the relationship between pretreatment overweight/obesity, adipose tissue distribution, and long-term prognosis of gastric cancer.

Methods: A total of 607 gastric cancer patients were involved in the retrospective cohort study. Overweight/obese patients were defined as body mass index (BMI) greater than 25 kg/m, and adipose tissue distribution parameters, including visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), and VAT/SAT ratio were measured at the level of the third lumbar vertebra using computerized tomography images within 15 days before the surgery. Multiple Cox regression models were applied to evaluate the association between overweight/obesity and disease-specific survival (DSS) of gastric cancer, and covariates including age, gender, T stage, N stage, and chemotherapy were adjusted. Furthermore, multiple Cox regression models were performed to evaluate the association between adipose tissue distribution parameters and DSS of gastric cancer; except for covariates mentioned above, overweight/obesity was adjusted additionally.

Results: Overweight/obesity was a predictive factor (HR = 0.61, 95% CI: 0.37-0.99) for the prognosis of gastric cancer. After additionally adjusting for overweight/obesity, high SAT percentage was an independent protective factor (HR = 0.59, 95% CI: 0.36-0.96), while high VAT percentage (HR = 1.68, 95% CI: 1.06-2.68) and high VAT/SAT ratio (HR = 1.99, 95% CI: 1.19-3.34) were independent risk factors for DSS of gastric cancer. Compared with other patients (overweight/obesity with low VAT/SAT ratio group, non-overweight/obesity or high VAT/SAT ratio group), patients in the non-overweight/obesity with high VAT/SAT ratio group had a worse prognosis (HR = 1.89, 95% CI: 1.28-2.77).

Conclusion: These results suggest that overweight/obesity is a predictive factor for the prognosis of gastric cancer. The VAT/SAT ratio could be used as a promising prognostic factor for gastric cancer. Therefore, in preoperative evaluation of gastric cancer patients, attention should be paid not only to BMI but also to adipose tissue distribution.
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http://dx.doi.org/10.3389/fonc.2021.680190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264507PMC
June 2021

Survival of viable but nonculturable Cronobacter sakazakii in macrophages contributes to infections.

Microb Pathog 2021 Jun 24;158:105064. Epub 2021 Jun 24.

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Wu Shan Road 483, Guangzhou 510642, PR China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong, PR China. Electronic address:

Cronobacter sakazakii (C. sakazakii), a pathogen that exists in dry and low-moisture environments, such as powder infant formula (PIF), can enter a viable but nonculturable (VBNC) state under harsh conditions, which enables it to escape traditional detection methods and thus poses a potential public health risk. This study aimed at assessing the virulent nature of VBNC C. sakazakii. Our results showed that VBNC C. sakazakii induced intestinal inflammation in neonatal rats. However, the degree of inflammation was significantly lower than that of culturable bacteria due to decreasing endotoxin production, motility, adhesion, and invasion ability in the VBNC state. From the perspective of bacterial translocation, the numbers of C. sakazakii in the blood, liver, and spleen of rats treated with VBNC cells were in the same order of magnitude as those treated with its culturable counterpart and may lead to the same degree of bacteremia. According to the macrophage survival assays, the survival rate of VBNC C. sakazakii within macrophages was 4.7 times higher than that of culturable cells. Based on these findings, we hypothesize that VBNC C. sakazakii evaded the host immune defense system, penetrated the tissue barrier, and translocated to the bloodstream, liver, and spleen through macrophages. Thus, our study reveals that VBNC C. sakazakii could be a potential risk for infants' health.
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http://dx.doi.org/10.1016/j.micpath.2021.105064DOI Listing
June 2021

Quantitative detection of trace VBNC Cronobacter sakazakii by immunomagnetic separation in combination with PMAxx-ddPCR in dairy products.

Food Microbiol 2021 Oct 19;99:103831. Epub 2021 May 19.

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

One immunomagnetic separation (IMS) assay based on immunomagnetic beads (IMBs) has been evaluated as a potential pretreatment tool for the separation and enrichment of target bacteria. In this study, we successfully immobilized antibodies onto magnetic bead surfaces to form IMBs through biotin and a streptavidin (SA) system to capture viable but nonculturable (VBNC) Cronobacter sakazakii (C. sakazakii) from dairy products. Various parameters that affected the capture efficiency (CE) of IMS, including the number of antibodies, IMBs dose, incubation time, magnetic separation time, and immunoreaction temperature, were systematically investigated. We further determined the optimal enrichment conditions for different dairy substrates to ensure maximum enrichment of target pathogens in the system. An IMS technique combining improved propidium monoazide (PMAxx) and droplet digital PCR (ddPCR) was established to detect the pathogenic VBNC C. sakazakii. The IMS-PMAxx-ddPCR method after IMBs enrichment showed higher accuracy when the VBNC C. sakazakii was under 1 Log copies/g. The detection limit for this method in a background of powdered infant formula (PIF) was 5.6 copies/g. In summary, the developed IMS-PMAxx-ddPCR method has great potential for the analysis and detection of VBNC bacteria in food.
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http://dx.doi.org/10.1016/j.fm.2021.103831DOI Listing
October 2021

Supplementation with soy isoflavones alleviates depression-like behaviour reshaping the gut microbiota structure.

Food Funct 2021 Jun;12(11):4995-5006

College of Food Science, South China Agricultural University, Guangzhou, 510642, China. and Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, South China Agricultural University, Guangzhou, 510642, China.

Soy isoflavones (SI) are known for their beneficial effects in alleviating neurodegenerative diseases, while the mechanism of alleviation of depression-like behaviour by SI remains unclear. In this study, a chronic unpredictable mild stress (CUMS)-induced depression rat model was used to determine the effect of SI in alleviating depression-like behaviour and its possible mechanisms. SI supplements significantly improved the CUMS-induced depression-like behaviour by increasing the monoamine neurotransmitter levels. A specific SI dose significantly modulated the composition of the gut microbiota, which in turn improved the maximum biotransformation ability of SI. Spearman's correlation analysis illustrated that some of the gut microbiota genera were strongly correlated with monoamine neurotransmitters. Moreover, more attention should be paid to gender differences, which may be related to changes in the gut microbiota. These results suggest that SI might affect monoamine neurotransmitters of CUMS rats by reshaping the structure of the gut microbiota, thereby alleviating depression-like behaviour.
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http://dx.doi.org/10.1039/d0fo03254aDOI Listing
June 2021

A case report of primary pulmonary meningioma masquerading as lung metastasis in a patient with rectal carcinoma: role of F-FDG PET/CT.

J Cardiothorac Surg 2021 May 29;16(1):153. Epub 2021 May 29.

Ningbo PET/CT center, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, China.

Background: Primary pulmonary meningioma (PPM) is an extremely rare disease, which is often misdiagnosed as lung metastasis. Previous studies indicated that PPM usually showed homogeneous enhancement on enhanced CT and high uptake of F-fluorodeoxyglucose (F-FDG) on positron emission tomography/CT (PET/CT). In this study, we report a case of PPM with atypical enhanced CT and F-FDG PET/CT features in a patient with rectal carcinoma.

Case Presentation: A 70-year-old male was demonstrated to have rectal carcinoma by biopsy while a solitary pulmonary nodule (SPN) with well-defined edges measuring 13 × 13 × 15 mm was almost simultaneously found in the right lower robe on chest CT scan. Contrast-enhanced CT and PET/CT revealed mild centripetal enhancement of the nodule without accumulation of F-FDG. A thoracoscopic wedge resection of the right lower lobe was finally performed and histopathologic examinations and PET/CT imaging showed that the nodule was a PPM.

Conclusion: PPM is a rare disease with heterogeneity not only in blood supply but also in glucose metabolism. F-FDG PET/CT may be an effective method for differentiating benign and malignant SPNs. The diagnosis of PPM depends on pathological and radiological examinations.
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http://dx.doi.org/10.1186/s13019-021-01546-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164743PMC
May 2021

Thymic transcriptome analysis after Newcastle disease virus inoculation in chickens and the influence of host small RNAs on NDV replication.

Sci Rep 2021 May 13;11(1):10270. Epub 2021 May 13.

Henan University of Animal Husbandry and Economy, No. 2 Yingcai Street, Huiji District, Zhengzhou, 450044, China.

Newcastle disease (ND), caused by virulent Newcastle disease virus (NDV), is a contagious viral disease affecting various birds and poultry worldwide. In this project, differentially expressed (DE) circRNAs, miRNAs and mRNAs were identified by high-throughput RNA sequencing (RNA-Seq) in chicken thymus at 24, 48, 72 or 96 h post LaSota NDV vaccine injection versus pre-inoculation group. The vital terms or pathways enriched by vaccine-influenced genes were tested through KEGG and GO analysis. DE genes implicated in innate immunity were preliminarily screened out through GO, InnateDB and Reactome Pathway databases. The interaction networks of DE innate immune genes were established by STRING website. Considering the high expression of gga-miR-6631-5p across all the four time points, DE circRNAs or mRNAs with the possibility to bind to gga-miR-6631-5p were screened out. Among DE genes that had the probability to interact with gga-miR-6631-5p, 7 genes were found to be related to innate immunity. Furthermore, gga-miR-6631-5p promoted LaSota NDV replication by targeting insulin induced gene 1 (INSIG1) in DF-1 chicken fibroblast cells. Taken together, our data provided the comprehensive information about molecular responses to NDV LaSota vaccine in Chinese Partridge Shank Chickens and elucidated the vital roles of gga-miR-6631-5p/INSIG1 axis in LaSota NDV replication.
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http://dx.doi.org/10.1038/s41598-021-89464-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8119446PMC
May 2021

Mycoplasma synoviae induces serum amyloid A upregulation and promotes chicken synovial fibroblast cell proliferation.

Microb Pathog 2021 May 13;154:104829. Epub 2021 Mar 13.

Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China; National Center for Engineering Research of Veterinary Bio-products, Jiangsu Academy of Agricultural Sciences, Nanjing, China. Electronic address:

Mycoplasma synoviae (MS) infection causes infectious synovitis and arthritis with hyperplasia of synovial cells in the chicken joint. However, its mechanism is unknown. We used primary chicken synovial fibroblast (CSF) as the research object to study the role of MS in the proliferation of MS-infected CSF and determine the mechanisms involved. Using integrated transcriptomic and proteomic analyses of the interaction between CSF and MS, we screened a proliferation-regulated factor, serum amyloid A (SAA), that may regulate proliferation of MS-infected CSF. SAA appears to be associated with MS-induced CSF proliferation. To study the role of SAA in MS-induced CSF proliferation, a eukaryotic expression vector overexpressing SAA and a small interfering RNA (siRNA) targeting Saa were constructed to manipulate the expression of SAA. Cell proliferation and apoptosis were detected via cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU), or terminal deoxyribonucleotidyl transferase-mediated dUTP nick-dnd labeling (TUNEL) assays, respectively. Western blot analysis was used to examine the protein expression level of SAA, cyclin E1, and cyclin-dependent kinase 2 (CDK2). In vitro, MS significantly promoted the proliferation of CSF and increased the production of SAA. Overexpression of SAA accelerated the proliferative ability of CSF, whereas knockdown of SAA depressed the proliferative ability of CSF. A TUNEL assay indicated that MS did not induce apoptosis. Silencing of SAA suppressed the expression of cyclin E1 and CDK2. These results suggest that MS may upregulate the expression of SAA, accelerate the cell cycle, and promote proliferation of CSF.
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http://dx.doi.org/10.1016/j.micpath.2021.104829DOI Listing
May 2021

Musculoskeletal ultrasound diagnosis of quadrilateral space syndrome: A case report.

Medicine (Baltimore) 2021 Mar;100(10):e24976

Department of Ultrasound, Baoji High-tech Hospital, Baoji, Shaanxi, China.

Introduction: Quadrilateral space syndrome (QSS) is a peripheral nerve entrapment disease, which can be misdiagnosed in clinic. In the past, QSS was mainly diagnosed by clinical symptoms combined with magnetic resonance imaging (MRI), electromyography (EMG), and arterial angiography. There are few reports on the diagnosis of QSS by musculoskeletal ultrasound (MSKUS) combined with clinical symptoms.

Patient Concerns: A middle-aged female patient had posterolateral pain and numbness in her right shoulder for 2 months.

Diagnoses: At first, she was diagnosed as suprascapular nerve entrapment, while EMG of suprascapular nerve and axillary nerve indicated that nerve conduction was normal. Then, MRI was performed, showing the shoulder had no abnormalities, and EMG and arterial angiography of upper limb showed no abnormalities too. Finally, she was diagnosed as QSS according to MSKUS and lidocaine block test.

Interventions: Two sealing treatments of axillary nerve block in quadrilateral space under the guidance of MSKUS were performed.

Outcomes: After 2 treatments, the pain and numbness in her shoulder disappeared, and her shoulder could move normally. There was no recurrence after 3 months of follow-up.

Conclusion: MSKUS is an effective method to diagnose QSS. It is fast, convenient and inexpensive, and is worth popularizing in clinic.
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http://dx.doi.org/10.1097/MD.0000000000024976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969238PMC
March 2021

Comparative Genomics of and New Targets for Molecular Diagnostics.

Front Vet Sci 2021 19;8:640067. Epub 2021 Feb 19.

Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

is an important pathogen of poultry, causing significant economic losses in this industry. Analysis of the unique genes and shared genes among different strains and among related species is helpful for studying the molecular pathogenesis of and provides valuable molecular diagnostic targets to facilitate the identification of species. We selected a total of 46 strains, including six strains, from 25 major animal (including avian) species/subspecies that had complete genome sequences and annotation information published in GenBank, and used them for comparative genomic analysis. After analysis, 16 common genes were found in the 46 strains. Thirteen single-copy core genes and the 16s rRNA genes were used for genetic evolutionary analysis. was found to have a distant evolutionary relationship not only with other arthritis-causing mycoplasmas, but also with another major avian pathogen, , that shares the major virulence factor with . Subsequently, six unique coding genes were identified as shared among these strains that are absent in other species with published genome sequences. Two of the genes were found to be located in the genetically stable regions of the genomes of and were determined to be present in all isolated strains ( = 20) and -positive clinical samples ( = 48) preserved in our laboratory. These two genes were used as molecular diagnostic targets for which SYBR green quantitative PCR detection methods were designed. The two quantitative PCR methods exhibited good reproducibility and high specificity when tested on positive plasmid controls and genomic DNA extracted from different strains, other major avian pathogenic bacteria/mycoplasmas, and low pathogenic Mycoplasma species. The detection limit for the two genes was 10 copies or less per reaction. The clinical sensitivity and specificity of the quantitative PCR methods were both 100% based on testing chicken hock joint samples with positive or negative infection. This research provides a foundation for the study of species-specific differences and molecular diagnosis of .
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http://dx.doi.org/10.3389/fvets.2021.640067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933220PMC
February 2021

Radiology residency training in China: results from the first retrospective nationwide survey.

Insights Imaging 2021 Feb 17;12(1):25. Epub 2021 Feb 17.

Vanke School of Public Health, Tsinghua University, Haidian District, Beijing, 100084, China.

Objectives: This was the first study to systematically landscape and examine China's nationwide standardized residency training in radiology.

Methods: In this retrospective cross-sectional study, we used data from the 2019 national survey of the first two cohorts of 3679 radiology residents who completed training in 2017 and 2018 across all 31 provinces in China. A total of 1163 (32%) residents participated in the survey. Multivariable logistic regression was used to examine the implementation frequency of 24 identified training tasks (categorized into six competencies) by region, demographics, and other residency information.

Results: Among the 1163 respondents, 592 (51%) were trained in the more developed eastern region. Of the 24 identified training tasks, 15 were implemented significantly differently across regions, while the frequency of the most frequently conducted tasks (e.g., CT, MR, and radiograph interpretation and reporting) was consistent. The top 10 tasks all fell into the patient care and medical knowledge competency domains, while other competencies tended to be neglected. We found region and marital status were the most influential factors of training task implementation frequencies. Respondents trained in the northeast and the west were more likely to report, for instance, radiological examination recommendation (OR = 1.91, 95%CI = 1.27-2.88), as "very frequent." Married respondents were more likely to report first-line night shift as "very frequent" (OR = 1.71, 95%CI = 1.29-2.26).

Conclusions: Despite the fast-win achievements of developing a national radiology residency training program, there is a gap to train quality and homogeneous radiologists across regions. Future improvement should be more tailored to residents' personal characteristics and emphasize some "soft" competencies (e.g., communication skills).
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http://dx.doi.org/10.1186/s13244-021-00970-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889775PMC
February 2021

Interaction of Mycoplasma synoviae with chicken synovial sheath cells contributes to macrophage recruitment and inflammation.

Poult Sci 2020 Nov 25;99(11):5366-5377. Epub 2020 Aug 25.

Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China; National Center for Engineering Research of Veterinary Bio-Products, Jiangsu Academy of Agricultural Sciences, Nanjing, China. Electronic address:

Mycoplasma synoviae (MS) is an important avian pathogen causing considerable economic hardship in the poultry industry. A major inflammation caused by MS is synovitis that occurs in the synovial tendon sheath and joint synovium. However, the overall appearance of pathological changes in the tendon sheath and surrounding tissues caused by MS infection at the level of pathological tissue sections was poor. Studies on the role of MS and synovial sheath cells (SSCs) interaction in the development of synovitis have not been carried out. Through histopathological observation, our study found that a major MS-induced pathological change of the tendon sheath synovium was extensive scattered and focal inflammatory cell infiltration of the tendon sheath synovial layer. In vitro research experiments revealed that the CFU numbers of MS adherent and invading SSC, the levels of expression of various pattern recognition receptors, inflammatory cytokines, and chemokines coding genes, such as IL-1β, IL-6, IL-8, CCL-20, RANTES, MIP-1β, TLR7, and TLR15 in SSCs, and chemotaxis of macrophages were significantly increased when the multiplicity of infection (MOI) of MS to SSC were increased tenfold. The expression level of IL-12p40 in SSC was significantly higher when the MOIs of MS to SSC were increased by a factor of 100. The interaction between MS and SSC can activate macrophages, which was manifested by a significant increase in the expression of IL-1β, IL-6, IL-8, CCL-20, RANTES, MIP-1β, and CXCL-13. This study systematically demonstrated that the interaction of MS with chicken SSC contributes to the inflammatory response caused by the robust expression of related cytokines and macrophage chemotaxis. These findings are helpful in elucidating the molecular mechanism of MS-induced synovitis in chickens.
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http://dx.doi.org/10.1016/j.psj.2020.08.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647830PMC
November 2020

Development and application of a real-time loop-mediated isothermal amplification method for quantification of Acetobacter aceti in red wine.

FEMS Microbiol Lett 2020 10;367(19)

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.

This study reports the development and optimization of a real-time loop-mediated isothermal amplification (qLAMP) method for rapid detection of Acetobacter aceti strain in red wine samples. Our results showed that the primers and probes designed for 16S rRNA were effective for A. aceti detection. The quantification limit of real-time polymerase chain reaction (qPCR) and qLAMP in pure culture was 2.05 × 101 colony forming units (CFU) mL-1. qLAMP had a sensitivity of 6.88 × 101 CFU mL-1 in artificially contaminated Changyu dry red wine (CDRW) and Changyu red wine (CRW), and 6.88 × 102 CFU mL-1 in artificially contaminated Greatwall dry red wine (GDRW), which was 10 times higher than that of qPCR. In conclusion, this newly developed qLAMP is a reliable, rapid and accurate method for the detection and quantification of A. aceti species in red wine samples. Furthermore, our work provides a standard reference method for the quantitative detection of A. aceti and other acetic acid bacteria during the fermentation and storage of red wine samples.
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http://dx.doi.org/10.1093/femsle/fnaa152DOI Listing
October 2020

Treatment for Liver Tumor Using Combined Transarterial Embolization and Interaarterial Transfecting HIF-1α shRNA in a Rabbit VX2 Model.

Onco Targets Ther 2020 24;13:8511-8519. Epub 2020 Aug 24.

Department of Radiology, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province 210029, People's Republic of China.

Background: Hypoxia-inducible factor-1α (-1α) has been selected as therapeutic gene in gene therapy. The aim of this study was to explore the treatment effect of combined transarterial embolization using microsphere treatment (MD) and intraarterial transfecting HIF-1α shRNA on hepatocellular carcinoma (HCC).

Materials And Methods: Rabbit skin fibroblast was transfected with -1α shRNA to evaluate the knocking down efficiency. Sixteen rabbit VX2 liver tumor models were randomly divided into four groups: the control group without any treatment, the MD group, the shRNA group (HIF-1α shRNA transfection by transcatheter intraarterial infusion), and the shRNA+MD group. The necrotic score, mitotic count and expression of HIF-1α, vascular endothelial growth factor (VEGF), CD34 and periodic acid-Schiff (PAS) stain were evaluated at the 14th and 28th day after treatment. The expression of HIF-1α and VEGF of VX2 tumors was also evaluated by real-time polymerase chain reaction on the 28th day.

Results: The expression of -1α-mRNA was lower in HIF-1α shRNA group than the control (p < 0.01). The tumor size was smaller in the shRNA + MD group than the shRNA group and the MD group (p < 0.05) on the 28th day. The growth rate of tumors in the shRNA + MD group was also lower than in other groups. The gene and protein expressions of both HIF-1α and VEGF in the shRNA + MD group were lower than the MD group, shRNA group and control group on the 28th day (p < 0.05). The necrotic score was higher in the shRNA + MD group than the MD group and control group (p < 0.05). The mitotic count and PAS-positive cells in shRNA + MD group were lower and CD34 was higher than the other three groups (p < 0.05).

Conclusion: Compared to therapy with MD or HIF-1α shRNA with transcatheter intraarterial transfection alone, the combined treatment has a better effect on HCC.
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http://dx.doi.org/10.2147/OTT.S262434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455770PMC
August 2020

Survival strategy of Cronobacter sakazakii against ampicillin pressure: Induction of the viable but nonculturable state.

Int J Food Microbiol 2020 Dec 6;334:108819. Epub 2020 Aug 6.

Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address:

In a viable but nonculturable (VBNC) state, bacteria are no longer culturable on standard laboratory media, but still, remain a pathogenic potential and present possible health risks. In this study, we investigated ampicillin's ability, which is commonly used in dairy cattle disease treatment, to induce Cronobacter sakazakii into the VBNC state. After treatment with ampicillin, the counts of culturable cells decreased from 10 CFU/mL to an undetected level 7-30 days post-treatment. Meanwhile, viable cells were still approximately 10-10 cells/mL, and could be resuscitated under appropriate conditions. Fluorescence microscopy showed that VBNC cell maintained apparent cellular integrity, but that the morphology of VBNC cells differed visibly from that of normal cells. Moreover, the respiratory chain activity of VBNC cells were confirmed by flow cytometry (FCM) analysis, suggesting that cells in a VBNC state were physiologically active. Finally, transcriptomics analysis and real-time PCR (qPCR) validation were used to explore the underlying mechanisms of VBNC cell formation. Over-expression of relA, lon, ppx, and ppk in the toxin-antitoxin (TA) trigger system contributed to VBNC cell formation. In the TA trigger system, RelA and exopolyphosphatases/guanosine pentaphosphate phosphohydrolases (PPX/GPPA) synthesize ppGpp, which activates polyphosphate kinase (PPK), the cellular enzyme that accumulates plyphosphate (PolyP). PolyP combines with and stimulates Lon to degrade the antitoxins, thereby activating the toxins that induce a VBNC state. The results of our research will facilitate a better understanding of the survival strategies that bacteria develop to deal with ampicillin pressure and the health risks associated with VBNC Cronobacter sakazakii induced by antibiotics.
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http://dx.doi.org/10.1016/j.ijfoodmicro.2020.108819DOI Listing
December 2020

Structural basis of DNA binding to human YB-1 cold shock domain regulated by phosphorylation.

Nucleic Acids Res 2020 09;48(16):9361-9371

State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan National Laboratory for Optoelectronics, Wuhan 430071, China.

Human Y-box binding protein 1 (YB-1) is a multifunctional protein and overexpressed in many types of cancer. It specifically recognizes DNA/RNA through a cold shock domain (CSD) and regulates nucleic acid metabolism. The C-terminal extension of CSD and the phosphorylation of S102 are indispensable for YB-1 function. Until now, the roles of the C-terminal extension and phosphorylation in gene transcription and translation are still largely unknown. Here, we solved the structure of human YB-1 CSD with a C-terminal extension sequence (CSDex). The structure reveals that the extension interacts with several residues in the conventional CSD and adopts a rigid structure instead of being disordered. Either deletion of this extension or phosphorylation of S102 destabilizes the protein and results in partial unfolding. Structural characterization of CSDex in complex with a ssDNA heptamer shows that all the seven nucleotides are involved in DNA-protein interactions and the C-terminal extension provides a unique DNA binding site. Our DNA-binding study indicates that CSDex can recognize more DNA sequences than previously thought and the phosphorylation reduces its binding to ssDNA dramatically. Our results suggest that gene transcription and translation can be regulated by changing the affinity of CSDex binding to DNA and RNA through phosphorylation, respectively.
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http://dx.doi.org/10.1093/nar/gkaa619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498358PMC
September 2020

Colonization Potential to Reconstitute a Microbe Community in Pseudo Germ-Free Mice After Fecal Microbe Transplant From Equol Producer.

Front Microbiol 2020 5;11:1221. Epub 2020 Jun 5.

College of Food Science, South China Agricultural University, Guangzhou, China.

Human intestinal microbiota plays a crucial role in the conversion of isoflavones into equol. Usually, human microbiota-associated (HMA) animal models are used, since it is difficult to establish the mechanism and causal relationship between equol and microbiota in human studies. Currently, several groups have successfully established HMA animal models that produce equol through germ-free mice or rats; however, the HMA model of producing equol through pseudo germ-free mice has not been established. The objective of this study is to establish an HMA mice model for equol production through pseudo germ-free mice, mimicking the gut microbiota of an adult human equol producer. First, a higher female equol producer was screened as a donor from 15 volunteers. Then, mice were exposed to vancomycin, neomycin sulfate, metronidazole, and ampicillin for 3 weeks to obtain pseudo germ-free mice. Finally, pseudo germ-free mice were inoculated with fecal microbiota of the equol producer for 3 weeks to establish HMA mice of producing equol. The results showed that (i) the ability to produce equol was partially transferred from the donor to the HMA mice. (ii) Most of the original intestinal microbiota of mice were eliminated after broad-spectrum antibiotic administration. (iii) The taxonomy data from HMA mice revealed similar taxa to the donor sample, and the species richness returned to the level close to the donor. (iv) The family and genera were successfully transferred from the donor to HMA mice. In conclusion, the HMA mice model for equol production, based on pseudo germ-free mice, can replace the model established by germ-free mice. The model also provides a basis for studying microbiota during the conversion from isoflavones into equol.
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http://dx.doi.org/10.3389/fmicb.2020.01221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7291926PMC
June 2020

A super-spreader of COVID-19 in Ningbo city in China.

J Infect Public Health 2020 Jul 7;13(7):935-937. Epub 2020 Jun 7.

Hwa Mei Hospital, University of Chinese Academy of Sciences, China; Institute of Life and Health Industry, University of Chinese Academy of Sciences, China. Electronic address:

An outbreak of Coronavirus Disease 2019 (COVID-19) has spread rapidly. It is imperative to control the epidemic by understanding the epidemiological feature, preventative quarantine, and effective hygiene measures. In the present study, we report a case of super-spreader who transmitted the disease to over twenty-eight persons in Ningbo, Zhejiang. Identifying and isolated super-spreaders, understanding the reasons behind the efficient transmission ability are important for the control and management of the ongoing COVID-19 pandemic.
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http://dx.doi.org/10.1016/j.jiph.2020.05.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275998PMC
July 2020

Reflection on SARS-CoV-2 infection of container ship seafarers.

Travel Med Infect Dis 2020 Jul - Aug;36:101787. Epub 2020 Jun 10.

Hwa Mei Hospital, University of Chinese Academy of Sciences, 315010, Ningbo, PR China; Ningbo Institute of Life and Health, University of Chinese Academy of Sciences, 315010, Ningbo, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.tmaid.2020.101787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283070PMC
September 2020

Development of a live attenuated duck hepatitis A virus type 3 vaccine (strain SD70).

Vaccine 2020 06 20;38(30):4695-4703. Epub 2020 May 20.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu Province, China; Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu Province, China. Electronic address:

Duck hepatitis A virus type 3 (DHAV-3) is an important pathogen that causes substantial losses in the Chinese duck industry. DHAV-3 is highly fatal to ducklings and there is no licensed vaccine in China available to reduce DHAV-3 infection. Our goal was to develop a live attenuated vaccine candidate against DHAV-3. A field isolated strain, SD, was attenuated by serially passaging in specific-pathogen-free (SPF) chicken embryos, and it lost its pathogenicity after 40 passages. The 70th passaged strain (SD70), which achieved good growth capacity in chicken embryos with a viral titer of 10 ELD/mL, was chosen to be the live attenuated vaccine candidate. The SD70 strain did not cause clinical signs of disease or mortality in 1-day-old ducklings and showed no virulence reversion after seven rounds of in vivo back passages. The minimum effective dose of SD70 was determined to be 10 ELD via the vaccination route of subcutaneous inoculation. A single dose of the SD70 provided good protection to susceptible ducklings against the lethal DHAV-3 strain. Compared with the genomic sequence of the parent SD strain, the SD70 had 12 amino acid substitutions, some of which may play a role in virulence attenuation. This study demonstrated that the attenuated SD70 strain is a promising vaccine candidate for the prevention of DHAV-3 infection in China. It exhibited safety, good stability and excellent protection.
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http://dx.doi.org/10.1016/j.vaccine.2020.05.030DOI Listing
June 2020

Computed tomography (CT) scan challenges the result of SARS-CoV-2 nucleic acid test in a suspected COVID-19 case.

Infect Control Hosp Epidemiol 2020 11 22;41(11):1362-1363. Epub 2020 Apr 22.

Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo, Zhejiang, China.

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http://dx.doi.org/10.1017/ice.2020.171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200842PMC
November 2020

Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and .

Front Microbiol 2020 3;11:576. Epub 2020 Apr 3.

Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

(MS), which causes respiratory disease, eggshell apex abnormalities, infectious synovitis, and arthritis in avian species, has become an economically detrimental poultry pathogen in recent years. In China, the disease is characterized by infectious synovitis and arthritis. However, the mechanism by which MS causes infectious synovitis and arthritis remains unknown. Increasing evidence suggests that synovial fibroblasts (SF) play a key role in the pathogenesis of arthritis. Here, both RNA sequencing and tandem mass tag analyses are utilized to compare the response of primary chicken SF (CSF) following infection with and without MS. The host response between non-infected and infected cells was remarkably different at both the mRNA and protein levels. In total, 2,347 differentially expressed genes (DEGs) (upregulated, = 1,137; downregulated, = 1,210) and 221 differentially expressed proteins (DEPs) (upregulated, = 129; downregulated, = 92) were detected in the infected group. A correlation analysis indicated a moderate positive correlation between the mRNA and protein level changes in MS-infected CSF. At both the transcriptomic and proteomic levels, 149 DEGs were identified; 88 genes were upregulated and 61 genes were downregulated in CSF. Additionally, part of these regulated genes and their protein products were grouped into seven categories: proliferation-related and apoptosis-related factors, inflammatory mediators, proangiogenic factors, antiangiogenic factors, matrix metalloproteinases, and other arthritis-related proteins. These proteins may be involved in the pathogenesis of MS-induced arthritis in chickens. To our knowledge, this is the first integrated analysis on the mechanism of CSF-MS interactions that combined transcriptomic and proteomic technologies. In this study, many key candidate genes and their protein products related to MS-induced infectious synovitis and arthritis were identified.
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http://dx.doi.org/10.3389/fmicb.2020.00576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147270PMC
April 2020

A miniaturized ECR plasma flood gun for wafer charge neutralization.

Rev Sci Instrum 2020 Mar;91(3):033319

State Key Laboratory of Nuclear Physics and Technology and Institute of Heavy Ion Physics, Peking University, Beijing 100871, People's Republic of China.

In modern ion implanters, a plasma flood gun (PFG) is used to neutralize wafer charge during the doping process, preventing the breakdown of floating wafers caused by the space charge accumulation. Typically, there are two kinds of PFGs, namely, dc arc discharge with filament and RF discharge. As a PFG, the filament one has limited lifetime and cannot avoid metallic contamination because of the thermal emitting filament. RF discharge PFG has been developed to solve these problems, including prolonging the source lifetime and avoiding metal pollution. Recently, a 2.45 GHz electron cyclotron resonance (ECR) ion source is also regarded as a potential choice for PFG. However, the dimension of the 2.45 GHz ECR source system including the size of the source itself and its meter's length RF subsidiary limits its application within an ion implanter. At Peking University, a miniaturized 2.45 GHz permanent magnet electron cyclotron resonance plasma flood gun with a coaxial RF transmission line has been built and tested. The dimensions of the ECR source body are Φ60 mm × Φ88 mm with a Φ30 mm × Φ40 mm plasma chamber. Its RF transmission line consists of a 200 W microwave generator, a 30 cm coaxial line, a 7 cm coaxial-to-waveguide transducer, and a microwave window that also serves as a vacuum seal. In continuous wave experiments, the electron extraction currents can be as high as 8.8 mA at an input RF power of 22 W with argon gas. The gas flow is less than 1.0 SCCM for this test.
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http://dx.doi.org/10.1063/1.5128522DOI Listing
March 2020

The preliminary test of multi-charged ions generation with a 2.45 GHz microwave-driven ion source.

Rev Sci Instrum 2020 Feb;91(2):023312

State Key Laboratory of Nuclear Physics and Technology and Institute of Heavy Ion Physics, Peking University, Beijing 100871, China.

A 2.45 GHz microwave-driven ion source for the generation of multicharged ions has been designed and built at Peking University recently. The magnetic field configuration of this ion source is a minimum-B type with a combination of a hexapole field and an axial mirror field. Argon was selected as the first tested beam generated by this ion source. A 63 µA Ar ion beam at 35 kV extraction voltage was obtained in the pulsed mode (50 Hz/500 µs). Without the hexapole magnetic field, the highest charge state was only Ar, and no Ar ion beam was detected. The comparison between the two sets of experimental results with different magnetic configurations has proven the rationality of the production of multicharged ions with this ion source. Both experimental results and discussion will be presented in this paper.
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http://dx.doi.org/10.1063/1.5128130DOI Listing
February 2020

Complete Genome Sequencing of Mycoplasma synoviae Strain HN01, Isolated from Chicken in Henan Province, China.

Microbiol Resour Announc 2020 Feb 6;9(6). Epub 2020 Feb 6.

Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China

Here, we report the complete genome sequence of HN01, a virulent epidemic strain isolated from a sick chicken with synovitis in Henan Province, China. HN01 is the Asian source of an strain that is completely sequenced, genome annotated, and published with relevant data.
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http://dx.doi.org/10.1128/MRA.01480-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005120PMC
February 2020

A unit-cell model for thermal regulation of degradation of organics in solid waste.

Waste Manag Res 2020 Jun 6;38(6):680-688. Epub 2020 Jan 6.

Geotechnical Engineering Research Institute, Hohai University, China.

It is a well appreciated fact that temperature is one of the key factors influencing the degradation of organics. Heat exchangers are a viable option that can be used to adjust the temperature in solid waste to an extent most suitable for waste degradation. This paper focuses on an experimental and theoretical investigation of the feasibility of using a water-circulating heat exchanger for thermal regulation of waste degradation. A cylindrical bioreactor with a central pipe connected to a water circulation system is devised and instrumented. The changes in temperature and gas production were monitored during the degradation of the organic component of the waste. Test results with and without thermal regulation are analyzed and compared. In addition, an analytical model is proposed to simulate the symmetrical heat transport behavior subjected to heat exchange. Heat generation due to the degradation of organics is taken into account. There was a good correlation between the analytical model prediction and the experimental data obtained from the laboratory test and field monitoring.
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http://dx.doi.org/10.1177/0734242X19893006DOI Listing
June 2020

Possibility of generating H, or H , or H dominated ion beams with a 2.45 GHz permanent magnet ECR ion source.

Rev Sci Instrum 2019 Dec;90(12):123305

State Key Laboratory of Nuclear Physics and Technology and Institute of Heavy Ion Physics, Peking University, Beijing 100871, China.

At Peking University (PKU), experimental research as well as theoretical study on how to produce high intense H, H , or H dominated ion beams with a compact permanent magnet 2.45 GHz electron cyclotron resonance (PMECR) ion source have been continuously carried out in the past few decades. Based on the comprehension of hydrogen plasma processes inside a 2.45 GHz PMECR discharge chamber, a three-phase diagram of ion fraction dominant regions that illustrates the relationship between the H, H , and H ion species and working parameters was presented. Meanwhile, a numerical model based on the particle population balance equations was developed for quantitative comprehension of electron cyclotron heated hydrogen plasma. Calculated results of H, H , and H fractions against gas pressure, microwave density, and wall material obtained with this numerical model agree well with the measured ones. Recently, a miniaturized ECR ion source has been developed, and a 52 mA hydrogen beam was extracted. Under the guidance of the model, H, H , and H beams with a fraction of 88%, 80%, and 82%, respectively, were obtained with this miniaturized ECR ion source under suitable working parameters. A PMECR ion source for a proton therapy facility has been built at PKU recently. A 34 mA beam H fraction of 91% was obtained at the first attempt.
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http://dx.doi.org/10.1063/1.5128019DOI Listing
December 2019

Comprehensive profiling of BRCA1 and BRCA2 variants in breast and ovarian cancer in Chinese patients.

Hum Mutat 2020 03 24;41(3):696-708. Epub 2019 Dec 24.

Novogene Co. Ltd., Beijing, China.

The identification and interpretation of germline BRCA1/2 variants become increasingly important in breast and ovarian cancer (OC) treatment. However, there is no comprehensive analysis of the germline BRCA1/2 variants in a Chinese population. Here we performed a systematic review and meta-analysis on such variants from 94 publications. A total of 2,128 BRCA1/2 variant records were extracted, including 601 from BRCA1 and 632 from BRCA2. In addition, 414, 734, 449, and 307 variants were also recorded in the BIC, ClinVar, ENIGMA, and UMD databases, respectively, and 579 variants were newly reported. Subsequent analysis showed that the overall germline BRCA1/2 pathogenic variant frequency was 5.7% and 21.8% in Chinese breast and OC, respectively. Populations with high-risk factors exhibited a higher pathogenic variant percentage. Furthermore, the variant profile in Chinese is distinct from that in other ethnic groups with no distinct founder pathogenic variants. We also tested our in-house American College of Medical Genetics-guided pathogenicity interpretation procedure for Chinese BRCA1/2 variants. Our results achieved a consistency of 91.2-97.6% (5-grade classification) or 98.4-100% (2-grade classification) with public databases. In conclusion, this study represents the first comprehensive meta-analysis of Chinese BRCA1/2 variants and validates our in-house pathogenicity interpretation procedure, thereby providing guidance for further PARP inhibitor development and companion diagnostics in the Chinese population.
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http://dx.doi.org/10.1002/humu.23965DOI Listing
March 2020

Imaging Features of Sclerosing Angiomatoid Nodular Transformation in Spleen.

J Comput Assist Tomogr 2019 Nov/Dec;43(6):863-869

From the Department of Radiology, the First Affiliated Hospital, College of Medicine, Jiaxing University.

Purpose: The purpose of this study was to evaluate the features of sclerosing angiomatoid nodular transformation (SANT) in spleen on the imaging of computed tomography (CT) and magnetic resonance (MR).

Materials And Methods: From July 2006 to April 2017, 12 patients with SANT confirmed by pathology were evaluated in a retrospective study. Eight patients were with CT imaging only, 2 patients were with MR imaging only, and 2 patients were with both CT and MR. Three professional senior radiologists analyzed the imaging features on CT and MR. The main characteristic analysis included size, margin, density, signal intensity, and enhancement pattern. The significant enhancement was defined as the degree of enhancement of lesion that is higher than the surrounding spleen parenchyma, and the mild enhancement was defined as the degree of enhancement of lesion that is lower than the surrounding spleen parenchyma.

Results: All the 12 patients (5 men, 7 women; mean age, 45.8 years; age range, 21-62 years) presented as single lesion without special clinical symptoms. The range of lesions on diameter was from 25 to 80 mm. On CT images, 9 (90%) of 10 presented as hypodense in comparison with the parenchyma of spleen, 1 (10%) of 10 presented as isodense, and calcification was observed in 4 (40%) of 10 cases. On MR images, 4 (100%) of 4 manifested heterogeneous hypointensity on in-phase sequence and 3 (75%) of 4 performed as isointensity on out-of-phase sequence of T1-weighted. On the sequences of T2-weighted and diffusion-weighted image, 4 (100%) of 4 showed hypointensity. On CT and MR enhancement images, the number of significant enhancement and mild enhancement was 2 and 10, respectively. Seven (58%) of 12 showed progressive enhancement with the pattern of "spoke-wheel."

Conclusions: Imaging features on CT and MR have a high diagnostic value for SANT, especially when CT combined with MR examination.
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http://dx.doi.org/10.1097/RCT.0000000000000910DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6867663PMC
November 2019