Publications by authors named "Jingbo Sun"

75 Publications

Ultrafast, Kinetically Limited, Ambient Synthesis of Vanadium Dioxides through Laser Direct Writing on Ultrathin Chalcogenide Matrix.

ACS Nano 2021 Jun 19;15(6):10502-10513. Epub 2021 May 19.

State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

Vanadium dioxide (VO) is a strongly correlated electronic material and has attracted significant attention due to its metal-to-insulator transition and diverse smart applications. Traditional synthesis of VO usually requires minutes or hours of global heating and low oxygen partial pressure to achieve thermodynamic control of the valence state. Further patterning of VO through a series of lithography and etching processes may inevitably change its surface valence, which poses a great challenge for the assembly of micro- and nanoscale VO-based heterojunction devices. Herein, we report an ultrafast method to simultaneously synthesize and pattern VO on the time scale of seconds under ambient conditions through laser direct writing on a VS "canvas". The successful ambient synthesis of VO is attributed to the ultrafast local heating and cooling process, resulting in controlled freezing of the intermediate oxidation phase during the relatively long kinetic reaction. A Mott memristor based on a VS-VO-VS lateral heterostructure can be fabricated and integrated with a MoS channel, delivering a transistor with abrupt switching transfer characteristics. The other device with a VSO channel exhibits a large negative temperature coefficient of approximately 4.5%/K, which is highly desirable for microbolometers. The proposed approach enables fast and efficient integration of VO-based heterojunction devices and is applicable to other intriguing intermediate phases of oxides.
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http://dx.doi.org/10.1021/acsnano.1c03050DOI Listing
June 2021

Broadband second-harmonic generation from artificial optical nonlinearity.

Opt Lett 2021 May;46(10):2368-2371

In this Letter, we present a mechanism for effectively broadening the bandwidth of second-harmonic generation (SHG) with the metamaterial-based artificial optical nonlinearity. As the nonlinear response of the artificial nonlinearity arising from the magnetoelectric coupling constructed by the meta-molecule (MM) structure, the broadband second-order nonlinearity can be built by simply combining the MMs with different geometrical sizes together. The physical model and the numerical simulation fully support the artificial generation and modulation of the broadband second harmonics. Our work suggests a new route for realizing the on-chip custom-designed nonlinear optical devices with broadband operation.
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http://dx.doi.org/10.1364/OL.423200DOI Listing
May 2021

Effects of compound deer bone extract on osteoporosis model mice and intestinal microflora.

J Food Biochem 2021 06 26;45(6):e13740. Epub 2021 Apr 26.

Department of Pharmaceutical Analysis, College of Pharmacy, Beihua University, Jilin, China.

The preventive and therapeutic mechanisms of CDBE on osteoporosis were studied by observing the serum bone-related biochemical indicators, bone trabecular micro-structure and intestinal flora in ovariectomized osteoporosis model mice, in order to provide a scientific theoretical basis for the further study on the effect of CDBE on osteoporosis, and the prevention and treatment of osteoporosis with clinical traditional Chinese medicines. The components in CDBE were detected by UHPLC-MS. A mouse osteoporosis model was established by the bilateral ovariectomy in female ICR mice. The biochemical indicators related to osteoporosis were detected, the right proximal tibia was scanned by Micro-CT, the intestinal microflora in the colon contents were examined, and the changes of microflora were taken as the main target to evaluate the effect of CDBE on the intestinal microflora in the model mice. A total of 16 compounds were obtained by the combined application of UHPLC-MS. CDBE could significantly increase the contents of E2, Ca , CT, HyP, OCN, FOXP3, P1NP and CTX-II, in the model mice. CDBE could significantly improve the trabecular micro-structure, Tb.N, Tb.Sp, SMI and Conn.D. CDBE could make the intestinal flora of osteoporosis model mice tend to healthy mice in species and quantity. CDBE can improve the symptoms of postmenopausal osteoporosis in mice, with a positive effect on the intestinal flora of postmenopausal mice. Its mechanism of regulating the symptoms of osteoporosis may be related to the regulation of bone-related biochemical indicators in the serum of mice. PRACTICAL APPLICATIONS: This research has a positive impact on the development of functional food with anti-osteoporosis in the future.
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http://dx.doi.org/10.1111/jfbc.13740DOI Listing
June 2021

Correction to: Mir-20a-5p induced WTX deficiency promotes gastric cancer progressions through regulating PI3K/AKT signaling pathway.

J Exp Clin Cancer Res 2021 Jan 28;40(1):46. Epub 2021 Jan 28.

Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510000, Guangdong Province, People's Republic of China.

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http://dx.doi.org/10.1186/s13046-020-01809-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841984PMC
January 2021

Oral Chinese Herbal Medicine as Prophylactic Treatment for Episodic Migraine in Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Evid Based Complement Alternat Med 2020 28;2020:5181587. Epub 2020 Dec 28.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine and Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, China.

Background: The prophylactic effects of Chinese herbal medicine (CHM) for migraine were examined in numerous clinical trials. This review aimed to analyze the effectiveness and safety of CHM as prophylactic treatment of migraine compared to flunarizine.

Methods: Nine databases were searched for randomized controlled trials (RCTs) that evaluated effects of CHM for episodic migraine prophylaxis compared to flunarizine, published before March 2019.

Results: Thirty-five RCTs with 2,840 participants met the inclusion criteria, and 31 of them were included in meta-analyses. The overall meta-analysis indicated that, when compared to flunarizine, CHM reduced the frequency of migraine attacks at the end of treatment (EoT) (21 studies, mean difference (MD) -1.23, 95% confidence interval (CI) (-1.69, -0.76)) and at the end of follow-up (EoFU) (five studies, MD -0.96, 95% CI (-1.70, -0.21)). Subgroup analyses based on the treatment duration, follow-up duration, and the dosage of flunarizine showed that CHM was superior to or comparable with flunarizine in reducing migraine frequency. Similar results were also found for secondary outcomes such as the pain visual analogue scale, migraine duration, responder rate, and acute medication usage. In particular, the studies that used CHM containing herb pairs (Chuan Xiong plus Bai Zhi and Chuan Xiong plus Tian Ma) showed promising results. However, the certainty of this evidence was evaluated as "low" or "very low" using the Grading of Recommendations, Assessment, Development and Evaluations approach.

Conclusion: CHM appeared to be comparable with flunarizine in reducing the frequency of episodic migraine attacks in adults at EoT and EoFU and well-tolerated by participants, regardless of the treatment duration, follow-up duration, and dosage of flunarizine. Due to the low certainty of the evidence, the suggested promising prophylactic outcomes require higher quality evidence from further rigorous RCTs.
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http://dx.doi.org/10.1155/2020/5181587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781685PMC
December 2020

Resveratrol induces human colorectal cancer cell apoptosis by activating the mitochondrial pathway via increasing reactive oxygen species.

Mol Med Rep 2021 03 5;23(3). Epub 2021 Jan 5.

Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Colorectal cancer (CRC) is the third most commonly diagnosed malignancy and the second leading cause of cancer‑related mortality worldwide according to Global Cancer Statistics 2018. Resveratrol (RSV) is a phenolic compound that possesses anticancer functions against various types of cancer, including breast and gastric cancer. However, the functions and mechanism underlying RSV in CRC are not completely understood. The present study aimed to investigate the anticancer effects and mechanism underlying RSV in CRC cells by conducting Cell Counting Kit‑8, apoptosis, reactive oxygen species (ROS) and western blotting assays. The results suggested that RSV dose‑dependently inhibited CRC cell viability, and increased cell apoptosis and ROS levels compared with the control group. The protein expression levels of Bax, cytochrome c, cleaved caspase‑9 and cleaved caspase‑3 were upregulated, whereas Bcl‑2 expression levels were downregulated in RSV‑treated CRC cells compared with control cells. The results indicated that RSV might activate the mitochondrial apoptotic pathway by increasing ROS release. The present study suggested that RSV possessed antitumour activity against CRC by modulating an ROS‑mediated mitochondrial apoptotic pathway.
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http://dx.doi.org/10.3892/mmr.2020.11809DOI Listing
March 2021

Agaricus blazei polypeptide exerts a protective effect on D-galactose-induced aging mice via the Keap1/Nrf2/ARE and P53/Trim32 signaling pathways.

J Food Biochem 2021 01 16;45(1):e13555. Epub 2020 Nov 16.

College of Pharmacy, Beihua University, Jilin, China.

This experiment mainly optimized the extraction technology of Agaricus blazei polypeptide (ABp) and evaluated its protective effect on aging mice. In this study, a novel single component, the M is 3 kD, was isolated and purified from Agaricus blazei. An aging mouse model was established using D-galactose. After the administration of ABp, the contents of total antioxidant capacity (T-AOC), malondialdehyde (MDA), catalase (CAT), and reactive oxygen species were significantly changed. Through immunofluorescence staining, it was observed that ABp can reduce changes in brain tissue. The differential expression of genes was analyzed by RNA-seq. A total of 295 differentially expressed genes were screened out in the ABp group.RT-qPCR verified important genes and showed that the mRNA expression levels of Hsph1, Trim32, HK1, Hnrnpa1, and Grik5 were significantly increased, and those of ApoE, Atp1a3, Stxbp1, and Mapk8ip1 was significantly decreased. Western blotting showed that the protein expression levels of Keap1 and p53 were significantly lower, while the protein expression levels of Nrf2, HO-1, Hsph1, and Trim32 were significantly higher in the ABP group. ABp played an anti-aging role in an aging mouse model. The specific mechanism of action may be related to the regulation of the expression of the Keap1/Nrf2/P53 signaling pathway and related factors. PRACTICAL APPLICATIONS: The research may contribute to the development of ABp as functional foods or dietary supplements for anti-aging in the future.
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http://dx.doi.org/10.1111/jfbc.13555DOI Listing
January 2021

Protocol of the Development of a Core Outcome Set for Ischemic Stroke in Clinical Trials of Chinese Medicine.

Evid Based Complement Alternat Med 2020 3;2020:2649843. Epub 2020 Oct 3.

The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.

. Ischemic stroke (IS) seriously impacts the quality of life of survivors. Chinese medicine (CM) has been developed for more than 2000 years and plays a key role in the treatment of ischemic stroke. Many Chinese medicine clinical trials have been conducted; however, the heterogeneity of outcome measurements and reporting limits implications of the findings in clinical practice and health policy development. Therefore, it is important to develop a core outcome set (COS) that should be used and reported in trials for ischemic stroke treated by Chinese medicine. This protocol describes the process of developing the IS-CM-COS. . The development of the COS will involve the following four steps: (1) A list of outcomes reported in the registered and published Chinese medicine trials of ischemic stroke will be extracted by conducting a systematic literature review. (2) An additional outcome list will be collected by semistructured interview to patients with ischemic stroke. (3) A two-round Delphi survey will be performed to prioritize and condense the outcomes. (4) In the consensus meeting, a final recommended COS will be developed. . The COS could improve the reliability and consistency of outcome reporting. We hope that this IS-CM-COS will be used in the future Chinese medicine trials for the treatment of ischemic stroke and improve research quality. . This study was registered with the Core Outcome Measures in Effectiveness Trials Initiative (http://www.comet-initiative.org/studies/details/1282).
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http://dx.doi.org/10.1155/2020/2649843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7556059PMC
October 2020

Mir-20a-5p induced WTX deficiency promotes gastric cancer progressions through regulating PI3K/AKT signaling pathway.

J Exp Clin Cancer Res 2020 Oct 8;39(1):212. Epub 2020 Oct 8.

1Department of Pathology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510000, Guangdong Province, People's Republic of China.

Background: The X-linked gene WTX (also called AMER1) has been reported to function as a tumour suppressor gene in Wilms' tumour. In our previous study, WTX expression was shown to be significantly reduced in gastric cancer (GC), but the function and mechanism associated with WTX loss had yet to be fully elucidated.

Methods: WTX expression and clinical significance were father analyzed in GC and control normal gastric tissues, and validated in public databases. The candidate pathway which was regulated by WTX during GC progression was searched by KEGG pathway analysis. The miRNA which monitored WTX expression was screened by miRNA microarray. After verified the pathway and miRNA both in vitro and in vivo, the relationship of miRNA, WTX and the downstream pathway were analyzed by Western blot, immunohistochemistry, RT-PCR, Co-immunoprecipitation (Co-IP), and luciferase analyses.

Results: The results showed that WTX serves as a tumour suppressor gene in GC. The loss of WTX which is associated with the aggressiveness of GC by promoting GC cell proliferation in vitro and high metastasis in vivo. Furthermore, WTX expression was positively correlated with the overall survival of GC patients. Microarray assays, bioinformatics analysis, and verification experiments showed that WTX loss activates the PI3K/AKT/mTOR pathway and promotes GC cell proliferation and invasion. And the aberrant miR-20a-5p upregulation contributes to WTX loss in GC, which stimulates PI3K phosphorylation to activate PI3K/AKT/mTOR signaling pathway and promoted GC progression.

Conclusions: The results of the present study elucidated the mechanism of GC progression, which is at least partially caused by aberrant miR-20a-5p upregulation leading to the inhibition of WTX expression and PI3K/AKT/mTOR signaling pathway activation. These findings provide a comprehensive understanding of the action of the miR-20a-5p/WTX/PI3K/AKT/mTOR signaling pathway in the progression and metastasis of GC.
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http://dx.doi.org/10.1186/s13046-020-01718-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545863PMC
October 2020

GFPT2 promotes metastasis and forms a positive feedback loop with p65 in colorectal cancer.

Am J Cancer Res 2020 1;10(8):2510-2522. Epub 2020 Aug 1.

Department of Pathology, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong Province, People's Republic of China.

As a rate-limiting enzyme of the hexosamine biosynthesis pathway (HBP), which is responsible for glycosylation, Glutamine fructose-6-phosphate amidotransferase 2 (GFPT2) is involved in human breast and lung tumorigenesis. However, whether GFTP2 is associated with tumor metastasis remains unclear. Here, we found that GFPT2 promoted the proliferation, migration, invasion and metastasis of colorectal cancer (CRC) cells. Mechanically, p65 acted as an upstream transcription factor of GFPT2 and regulated its expression and function. In turn, GFPT2 enhanced the glycosylation of p65, which led to the nuclear translocation of p65 and then activated NF-κB pathway. Thus, GFTP2 and p65 formed a positive feedback loop to promote the progression of CRC. In addition, GFPT2 was up-regulated in CRC tissues and closely related with liver metastasis (P<0.0001) and tumor stage (P=0.0184). High expression of GFPT2 predicted poor prognosis for CRC patients. Moreover, GFTP2 expression was positively linked with O-linked N-acetylglucosamine transferase in CRC tissues. Our study reveals a new mechanism of GFPT2 in CRC metastasis and provides a new target therapeutic target to deter metastasis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471343PMC
August 2020

Tunable topological charge vortex microlaser.

Science 2020 05;368(6492):760-763

Department of Materials Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA.

The orbital angular momentum (OAM) intrinsically carried by vortex light beams holds a promise for multidimensional high-capacity data multiplexing, meeting the ever-increasing demands for information. Development of a dynamically tunable OAM light source is a critical step in the realization of OAM modulation and multiplexing. By harnessing the properties of total momentum conservation, spin-orbit interaction, and optical non-Hermitian symmetry breaking, we demonstrate an OAM-tunable vortex microlaser, providing chiral light states of variable topological charges at a single telecommunication wavelength. The scheme of the non-Hermitian-controlled chiral light emission at room temperature can be further scaled up for simultaneous multivortex emissions in a flexible manner. Our work provides a route for the development of the next generation of multidimensional OAM-spin-wavelength division multiplexing technology.
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http://dx.doi.org/10.1126/science.aba8996DOI Listing
May 2020

MicroRNA-451 Aggravates Kainic Acid-induced Seizure and Neuronal Apoptosis by Targeting GDNF.

Curr Neurovasc Res 2020 ;17(1):50-57

Department of Neurology, The Second Affiliated Hospital of Guizhou, Guizhou University of Traditional Chinese Medicine, Guiyang City, Guizhou Province, 550003, China.

Aim: Epilepsy is a common and serious neurological disease that causes recurrent episodes, but its molecular mechanism remains unclear. Abnormal miRNA expression is associated with epilepsy, including miR-451. This research investigated the role of miR-451 in seizure and its detailed mechanism.

Methods: The seizure mice model was induced by kainic acid (KA) injection to the right lateral cerebral ventricle. Behavioral changes in mice were observed and evaluated by the Racine Scale. The miR-451 knockout mice were established by adenovirus infection. The in vitro model was performed by miR-451 mimics transfected HEK-293 cells. The amount of neuronal death and morphological changes were evaluated by Nissl staining and H&E staining.

Results: The results showed that miR-451 is up regulated in KA-induced seizure models and miR- 451 knockout decreased the behavior score and improved the pathological changes of the hippocampus. Besides, MiR-451 knockout inhibited the apoptosis of hippocampal neurons. Bioinformatics studies have shown that glial cell line-derived neurotrophic factor (GDNF) is a target gene of miR-451. MiR-451 could negatively regulate the expression of GDNF. GDNF overexpression could reverse the effect of miR-451 on KA induced brain injury and neuronal apoptosis.

Conclusion: This research demonstrates that miR-451 can affect the behavior of KA-induced epilepsy mice and hippocampal neuronal damage by regulating GDNF expression. The results would provide an experimental foundation for further research about the potential contribution of mi- RNAs to epilepsy pathophysiology.
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http://dx.doi.org/10.2174/1567202617666191223150510DOI Listing
July 2021

Hydrocortisone Suppresses Early Paraneoplastic Inflammation And Angiogenesis To Attenuate Early Hepatocellular Carcinoma Progression In Rats.

Onco Targets Ther 2019 8;12:9481-9493. Epub 2019 Nov 8.

School of Pharmacy and Medical Sciences, and UniSA Cancer Research Institute, University of South Australia, Adelaide, SA 5001, Australia.

Background: Inflammation is implicated in both hepatic cirrhosis development and hepatocellular carcinogenesis, and treatment with long-acting glucocorticoid dexamethasone prevented liver carcinogenesis in mice. However, it is unclear whether glucocorticoids have anti-inflammatory effect on hepatocellular carcinoma (HCC) and if short-acting glucocorticoids (with fewer adverse effects) inhibit paraneoplastic inflammation and HCC progression.

Methods: To investigate whether different types of anti-inflammatory agents attenuate HCC progression, the current study compared effects of treatments with hydrocortisone (a short-acting glucocorticoid) or aspirin on HCC progression. HCC was induced in diethylnitrosamine-treated rats which were randomly divided into 4 groups (n=8), respectively receiving orally once daily vehicle, glucuronolactone, glucuronolactone+hydrocortisone, and glucuronolactone+aspirin. Diethylnitrosamine (DEN) was given to rats in drinking water (100mg/L) to induce HCC. At weeks 12 and 16 post-induction, effects were compared on HCC nodule formation, microvessel density, and macrophage infiltration, and levels of paraneoplastic protein expression of tumor necrosis factor (TNF)-α, p38 mitogen-activated protein kinase (p38), phosphorylated p38 (p-p38), nuclear factor (NF)-κB, interleukin (IL)-10, hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1 and vascular endothelial growth factor (VEGF).

Results: Compared to the model and glucuronolactone alone groups, HCC nodule number and microvessel density in the glucuronolactone+hydrocortisone group were significantly lower at week 12. At week 12 but not week 16, significantly lower levels of macrophages, TNF-α, p-p38, NF-κB, IL-10, HGF, TGF-β1 and VEGF were observed in the paraneoplastic tissue of the glucuronolactone+hydrocortisone group when compared with the control and glucuronolactone groups.

Conclusion: The results suggest that hydrocortisone treatment reduces macrophage polarization, expression of inflammatory and anti-inflammatory cytokines, and angiogenesis in paraneoplastic tissue, and attenuates early HCC progression. Although hydrocortisone did not have attenuation effect on advanced solid tumor, the current study shows the potential benefits and supports potential clinical use of hydrocortisone in attenuating early progression of HCC, which is through suppressing paraneoplastic inflammation and angiogenesis.
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http://dx.doi.org/10.2147/OTT.S224618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850701PMC
November 2019

Overexpression of CENPF correlates with poor prognosis and tumor bone metastasis in breast cancer.

Cancer Cell Int 2019 11;19:264. Epub 2019 Oct 11.

1Department of General Surgery, The Third Affiliated Hospital of Southern Medical University, 183 West Zhongshan Avenue, Guangzhou, 510630 Guangdong China.

Background: Centromere Protein F (CENPF) associates with the centromere-kinetochore complex and influences cell proliferation and metastasis in several cancers. The role of CENPF in breast cancer (BC) bone metastasis remains unclear.

Methods: Using the ONCOMINE database, we compared the expression of CENPF in breast cancer and normal tissues. Findings were confirmed in 60 BC patients through immunohistochemical (IHC) staining. Microarray data from GEO and Kaplan-Meier plots were used analyze the overall survival (OS) and relapse free survival (RFS). Using the GEO databases, we compared the expression of CENPF in primary lesions, lung metastasis lesions and bone metastasis lesions, and validated our findings in BALB/C mouse 4T1 BC models. Based on gene set enrichment analysis (GSEA) and western blot, we predicted the mechanisms by which CENPF regulates BC bone metastasis.

Results: The ONCOMINE database and immunohistochemical (IHC) showed higher CENPF expression in BC tissue compared to normal tissue. Kaplan-Meier plots also revealed that high CENPF mRNA expression correlated to poor survival and shorter progression-free survival (RFS). From BALB/C mice 4T1 BC models and the GEO database, CENPF was overexpressed in primary lesions, other target organs, and in bone metastasis. Based on gene set enrichment analysis (GSEA) and western blot, we predicted that CENPF regulates the secretion of parathyroid hormone-related peptide (PTHrP) through its ability to activate PI3K-AKT-mTORC1.

Conclusion: CENPF promotes BC bone metastasis by activating PI3K-AKT-mTORC1 signaling and represents a novel therapeutic target for BC treatment.
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http://dx.doi.org/10.1186/s12935-019-0986-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6788011PMC
October 2019

The add-on effect of dengzhan shengmai capsules on secondary prevention of ischemic stroke: A multicentre, randomised, placebo-controlled clinical trial.

Complement Ther Med 2019 Oct 24;46:189-194. Epub 2019 Aug 24.

Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China. Electronic address:

Objective: The Dengzhan Shengmai (DZSM) capsule is a commercially available type of Chinese herbal medicine frequently administered to improve neurological impairment after stroke. Its ability to prevent recurrent stroke, however, has not been determined. This study therefore evaluated the ability of DZSM as an add-on to conventional secondary preventive agents to prevent recurrent ischemic stroke.

Methods: In this randomised, double-blind, placebo-controlled trial, conducted at 83 hospitals in Mainland China, 3143 patients in 14-180 days after the initial onset of ischemic stroke, were randomly allocated to the DZSM (0.36 g, twice daily for 12 months) or the placebo group. All patients in both groups received standard secondary preventive medications. The primary outcome was the 1-year incidence of stroke. Between group differences were assessed using the Cox proportional hazards model.

Results: Intent-to-treat analysis showed that 58 (3.8%) participants in the DZSM group and 82 (5.4%) in the placebo group experienced new stroke events (hazard ratio = 0.70, 95% confidence interval = 0.50-0.98, P = 0.036). The type and incidence of adverse events were similar in the DZSM and placebo groups.

Conclusions: The addition of DZSM capsules to standard secondary preventive agents provides additional benefits after the initial onset of ischemic stroke, reducing recurrent stroke without increasing severe adverse events. However, further study is needed to elucidate the role of DZSM on the updated practice of conventional secondary prevention for ischemic stroke.
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http://dx.doi.org/10.1016/j.ctim.2019.08.015DOI Listing
October 2019

A new cerebroside from cordyceps militaris with anti-PTP1B activity.

Fitoterapia 2019 Oct 31;138:104342. Epub 2019 Aug 31.

College of Pharmacy, Beihua University, Jilin 132013, China. Electronic address:

Cordyceps militaris (L.) Link (C. militaris) has been used as a folk medicine for treatment of various diseases in China and some other countries. Recent evidence suggests that aqueous extracts of C. militaris have hypoglycemic activity. So the aim of this study was to isolate and characterize compounds with aiti-PTP1B (protein tyrosine phosphatase 1B) activity from C. militaris. As a result, cordycerebroside B (1) together with other three known cerebrosides (2-4) and a disaccharide (5) were isolated by silica gel column chromatography and semi-preparative high performance liquid chromatography (HPLC) and then elucidated on the basis of 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, mass spectroscopy (MS) and chemical method. Among of which, cordycerebroside B was a new compound and isolated from C. militaris for the first time. The results of the activity assays demonstrated that all these four cerebrosides (compounds 1-4) showed marked inhibition activity against PTP1B with IC values of 4.68 ± 0.18, 16.93 ± 1.08, 10.43 ± 0.64 and 18.92 ± 1.65 μM. All the compounds had no discernible cytotoxicity for Rat pheochromocytoma (PC12 cells). These findings suggested that C. militaris or its cerebrosides may be considered as potential useful therapeutic agents for type 2 diabetes.
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http://dx.doi.org/10.1016/j.fitote.2019.104342DOI Listing
October 2019

[Expression of NUF2 in breast cancer and its clinical significance].

Nan Fang Yi Ke Da Xue Xue Bao 2019 May;39(5):591-597

Department of General Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China.

Objective: To investigate the expression of the cell division- associated gene NUF2 in breast cancer and its clinical significance.

Methods: The expression of NUF2 in breast cancer tissues was analyzed using Oncomine database. The relationship between the expression of NUF2 and the prognosis of breast cancer was analyzed using the Kaplan-Meier Plotter database. Gene set enrichment analysis (GSEA) and GEO database were used to investigate the effect of NUF2 on gene enrichment. The String database was utilized to analyze the proteins associated with NUF2. The TIMER database was analyzed to assess the correlations of NUF2 with BUB1, MAD2L1 and MYC. The expressions of NUF2 mRNA in 8 pairs of breast cancer tissues and adjacent tissues were verified by q-PCR.

Results: Compared with that in normal breast tissue, NUF2 was significantly overexpressed in breast cancer ( < 0.001). The overall survival time (HR = 1.52, = 0.015) and the recurrence-free survival time (HR = 1.85, = 3.2e-14) of the patients with high NUF2 expression were significantly shorter than those of patients with low NUF2 expression. In patients with high NUF2 expression, the enriched genes were involved mainly in cell cycle, P53, G2/M, DNA repair, MYC, and PI3K-AKT-MTOR signaling pathways, which were associated with tumor proliferation, invasion, metastasis and stemness. Combination of the results of String database, gene enrichment and TIMER database analyses suggested that NUF2 interacted directly with BUB1, MAD2L1, and MYC, which could promote the progression of breast cancer. The results of q-PCR showed that NUF2 expression was up-regulated in 6 cancer tissues and down-regulated in 2 cancer tissues.

Conclusions: NUF2 gene is overexpressed in breast cancer, and its expression level is important in predicting the prognosis of breast cancer.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2019.05.15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6743945PMC
May 2019

FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression.

Cell Death Dis 2019 04 25;10(5):351. Epub 2019 Apr 25.

Department of Pathology, Southern Medical University, 510515, Guangzhou, Guangdong Province, P. R. China.

F-box only protein 8 (FBX8), as a critical component of the SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases, has been associated with several malignancies through interacting with a member of proteins. However, the substrates of FBX8 for destruction in the progression of colorectal carcinoma (CRC) need to be explored. Here, we show that loss of FBX8 accelerates chemical-induced colon tumorigenesis. FBX8 directly targets GSTP1 for ubiquitin-mediated proteasome degradation in CRC. GSTP1 promotes the proliferation, invasion, and metastasis of CRC cells. Furthermore, GSTP1 is upregulated in CRC tissue samples and predicts poor prognosis of CRC patients. The inactivation of FBX8 negatively correlated with increased levels and stability of GSTP1 in clinical CRC tissues and FBX8 knockout transgenic mice. These findings identify a novel ubiquitination pathway as FBX8-GSTP1 axis that regulates the progression of CRC, which might be a potential prognostic biomarker for CRC patients.
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http://dx.doi.org/10.1038/s41419-019-1588-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484082PMC
April 2019

Duodenojejunal Bypass Plus Sleeve Gastrectomy Reduces Infiltration of Macrophages and Secretion of TNF-α in the Visceral White Adipose Tissue of Goto-Kakizaki Rats.

Obes Surg 2019 06;29(6):1742-1750

Southern Medical University, Guangzhou, 510515, China.

Background: Current studies indicate that inflammation of white adipose tissue (WAT) is a pathogenic characteristic of insulin resistance. However, the significance of visceral WAT inflammation after bariatric surgery remains unclear.

Methods: Duodenojejunal bypass plus sleeve gastrectomy (DJB-SG) was performed on Goto-Kakisaki rats. Weight, fasting blood glucose (FBG), and homeostatic model assessment of insulin resistance (HOMA-IR) in the DJB-SG group were compared to those in a sham surgery (SHAM) group every 2 weeks. The results of an oral glucose tolerance test (OGTT) and the volume of visceral adipose tissue (Visc.Fat) were compared before and 8 weeks postsurgery. Eight weeks after surgery, the rats were sacrificed and visceral WAT collected from the greater omentum. Tumor necrosis factor-α (TNF-α) and cluster of differentiation 68 (CD68) expression in the WAT were evaluated in paraffin-embedded sections by immunohistochemistry.

Results: Compared with the SHAM group, the DJB-SG group demonstrated a significant reduction in weight, FBG, and HOMA-IR (P < 0.05), with elevation of insulin levels (P < 0.05) from 4 weeks after surgery. OGTT and the quantity of Visc.Fat were significantly reduced (P < 0.05) 8 weeks after surgery. Moreover, the expression of TNF-α and CD68 in the visceral white adipose tissue was significantly lower 8 weeks after surgery (P < 0.05).

Conclusions: The DJB-SG model established in Goto-Kakisaki rats achieved anticipated efficacy. Reduced TNF-α-related inflammation in visceral WAT may result in improved insulin resistance.
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http://dx.doi.org/10.1007/s11695-019-03755-1DOI Listing
June 2019

Cancer-derived exosomal miR-25-3p promotes pre-metastatic niche formation by inducing vascular permeability and angiogenesis.

Nat Commun 2018 12 19;9(1):5395. Epub 2018 Dec 19.

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong Province, People's Republic of China.

Cancer-derived exosomes are considered a major driver of cancer-induced pre-metastatic niche formation at foreign sites, but the mechanisms remain unclear. Here, we show that miR-25-3p, a metastasis-promoting miRNA of colorectal cancer (CRC), can be transferred from CRC cells to endothelial cells via exosomes. Exosomal miR-25-3p regulates the expression of VEGFR2, ZO-1, occludin and Claudin5 in endothelial cells by targeting KLF2 and KLF4, consequently promotes vascular permeability and angiogenesis. In addition, exosomal miR-25-3p from CRC cells dramatically induces vascular leakiness and enhances CRC metastasis in liver and lung of mice. Moreover, the expression level of miR-25-3p from circulating exosomes is significantly higher in CRC patients with metastasis than those without metastasis. Our work suggests that exosomal miR-25-3p is involved in pre-metastatic niche formation and may be used as a blood-based biomarker for CRC metastasis.
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http://dx.doi.org/10.1038/s41467-018-07810-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300604PMC
December 2018

Reconfiguring structured light beams using nonlinear metasurfaces.

Opt Express 2018 Nov;26(23):30930-30943

Ultra-compact, low-loss, fast, and reconfigurable optical components, enabling manipulation of light by light, could open numerous opportunities for controlling light on the nanoscale. Nanostructured all-dielectric metasurfaces have been shown to enable extensive control of amplitude and phase of light in the linear optical regime. Among other functionalities, they offer unique opportunities for shaping the wave front of light to introduce the orbital angular momentum (OAM) to a beam. Such structured light beams bring a new degree of freedom for applications ranging from spectroscopy and micromanipulation to classical and quantum optical communications. To date, reconfigurability or tuning of the optical properties of all-dielectric metasurfaces have been achieved mechanically, thermally, electrically or optically, using phase-change or nonlinear optical materials. However, a majority of demonstrated tuning approaches are either slow or require high optical powers. Arsenic trisulfide (AsS) chalcogenide glass offering ultra-fast and large χnonlinearity as well as a low two-photon absorption coefficient in the near and mid-wave infrared spectral range, could provide a new platform for the realization of fast and relatively low intensity reconfigurable metasurfaces. Here, we design and experimentally demonstrate an AsS chalcogenide glass based metasurface that enables reshaping of a conventional Hermite-Gaussian beam with no OAM into an OAM beam at low intensity levels, while preserves the original beam's amplitude and phase characteristics at high intensity levels. The proposed metasurface could find applications for a new generation of optical communication systems and optical signal processing.
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http://dx.doi.org/10.1364/OE.26.030930DOI Listing
November 2018

H- and J-aggregates formed from a nontraditional π-gelator depending on the solvent polarity for the detection of amine vapors.

Org Biomol Chem 2018 10;16(40):7438-7445

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P.R. China.

A tert-butyl carbazole-modified difluoroboron β-diketonate complex (TCbzB) has been synthesized. Although no traditional gelation group was involved in TCbzB, it could form organogels in the mixed solvents of o-dichlorobenzene/cyclohexane (v/v = 1/5 or 1/2), toluene/cyclohexane (v/v = 1/2) and chlorobenzene/cyclohexane (v/v = 1/2). Interestingly, an orange organogel was obtained in o-dichlorobenzene/cyclohexane (v/v = 1/2) with relatively high polarity and red organogels were gained in the other three mixed solvents with relatively low polarity. TCbzB self-assembled into H-aggregates and J-aggregates in orange and red organogels, respectively, and the corresponding xerogels emitted yellow and red light, respectively, under UV illumination. The red emission of the xerogel-based film could be quenched significantly by gaseous n-propylamine and aniline because of the decomplexation of the difluoroboron β-diketonate complex by n-propylamine and the weak interactions between aniline and boron difluoride units.
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http://dx.doi.org/10.1039/c8ob01596aDOI Listing
October 2018

Combined antitumor effects of P-5m octapeptide and 5-fluorouracil on a murine model of H22 hepatoma ascites.

Exp Ther Med 2018 Sep 6;16(3):1586-1592. Epub 2018 Jul 6.

Department of Microbial and Biochemical Pharmacy, College of Pharmaceutical Science, Beihua University, Changchun, Jilin 132013, P.R. China.

The present study has demonstrated that P-5m octapeptide (P-5m) has therapeutic potential in metastatic human hepatocarcinoma, possibly through the modulation of matrix metalloproteinase-2 expression. The purpose of the present study was to evaluate the antitumor effect of P-5m combined with 5-fluorouracil (5-Fu) on the treatment of hepatoma 22 (H22) hepatocarcinoma malignant ascites in a mouse model. The inhibitory effect on the growth of mouse ascites tumors was monitored by measuring body weight gain, survival time, ascites volume, numbers of tumor cells, DNA synthesis and peritoneal capillary permeability analysis. The present data revealed a significant reduction in ascites volume and cell count in mice that were treated with P-5m plus 5-Fu. Furthermore, the median survival time in mice in the combination group was prolonged compared with the disease control group. Moreover, a significant reduction in the total H22 ascites cell count in mice from the combination group was observed when compared with the disease control group. P-5m plus 5-Fu was able to induce the cell cycle arrest and inhibit the peritoneal capillary permeability of the mice. To conclude, the present study indicated that P-5m may have therapeutic potential in ascites caused by hepatocellular carcinoma.
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http://dx.doi.org/10.3892/etm.2018.6422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122418PMC
September 2018

Light-Induced Bending of Needle-Like Crystals of Naphthylvinylbenzoxazole Triggered by trans-cis Isomerization.

Chem Asian J 2018 Jul 6;13(13):1719-1724. Epub 2018 Jun 6.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, P.R. China.

New diarylethene derivatives containing benzoxazole (NBO) and benzothiazole (NBT) have been synthesized. Light-induced trans-cis isomerization of NBO and NBT took place in crystals, and only induced the needle-like crystals of NBO to bend backwards away from the UV light source. The movement of the atoms was deemed to take place during the isomerization of NBO; hence, strain would be produced and accumulated rapidly in the surface of crystals exposed to UV light. The uniform release of strain led to the bending of needle-like crystals. The light-induced trans-cis isomerization efficiency of NBT was too low to drive the motion of crystals, which might have originated from the large repulsion between naphthyl and benzothiazole. These results provide a new platform for the transformation of light energy into mechanical energy in molecular crystals through the unimolecular photochemical reaction of diarylethene derivatives.
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http://dx.doi.org/10.1002/asia.201800380DOI Listing
July 2018

TGF- Overexpression in Breast Cancer Bone Metastasis and Primary Lesions and TGF- Enhancement of Expression of Procancer Metastasis Cytokines in Bone Marrow Mesenchymal Stem Cells.

Biomed Res Int 2018 8;2018:6565393. Epub 2018 Feb 8.

Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia.

Bone metastasis (BM) is the advanced complication of breast cancer, while bone marrow-derived mesenchymal stem cells (BMSCs) in the microenvironment unclearly contribute to cancer metastasis. This study investigated potential roles of transforming growth factor- (TGF-) in the interaction between breast cancer and BMSCs in BM. Clinical cases of breast cancer with bone metastasis (BMBC), breast cancer without bone metastasis (Non-BM-BC), and benign fibroadenoma (Benign) were enlisted in a retrospective study. TGF- was found obviously overexpressed in BM lesion of BMBC compared to primary lesion of both BMBC and Non-BM-BC ( < 0.01), and TGF- was higher in primary lesion of both BMBC and Non-BM-BC ( < 0.01) than Benign group. Interestingly, TGF- in nontumor tissues of both BMBC and Non-BM-BC was at a higher level than Benign group ( < 0.01), and numbers of macrophages in nontumor tissues of both BMBC and Non-BM-BC ( < 0.01) were higher than Benign group. Furthermore, in cultured human BMSCs, TGF- stimulated production of procancer cytokines including IL-6, VEGF, FGF10, FGF17, and TGF-1 in a dose-dependent manner. Thus, TGF- in BC could potentially be an important signal of carcinogenesis and metastasis. Macrophages in the nontumor tissue of BC may not be protective but could promote cancer metastasis.
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http://dx.doi.org/10.1155/2018/6565393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5822790PMC
September 2018

Nanoscale orbital angular momentum beam instabilities in engineered nonlinear colloidal media.

Opt Express 2018 Mar;26(5):5118-5125

Colloidal media with well-defined optical properties have been widely used as model systems in many fundamental and applied studies of light-matter interactions in complex media. Recent progress in the field of engineered nanoscale optical materials with fundamentally new physical properties opens new opportunities for tailoring the properties of colloids. In this work, we experimentally demonstrate the evolution of the optical vortex beams of different topological charges propagating in engineered nano-colloidal suspension of negative polarizability with saturable nonlinearities. Due to the high power of the incident beam, the modulation instability leads to an exponential growth of weak perturbations and thus splits the original vortex beam into a necklace beam consisting of several bright spots. At a fixed power, the number of observed bright spots is intrinsically determined by the topological charge of the incident beam and agrees well with the predictions of our linear stability analysis and numerical simulations. Besides contributing to the fundamental science of light-matter interactions in engineered soft-matter media, this work opens new opportunities for dynamic optical manipulation and transmission of light through scattering media as well as formation of complex optical patterns and light filamentation in naturally existing colloids such as fog and clouds.
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http://dx.doi.org/10.1364/OE.26.005118DOI Listing
March 2018

Diarylethene-based xerogels: the fabrication of more entangled networks driven by isomerization and acidofluorochromism.

Org Biomol Chem 2018 03 7;16(12):2114-2124. Epub 2018 Mar 7.

Sate Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, P.R. China.

Fully-conjugated styrylbenzoxazoles and styrylbenzothiazoles of BOAF24, BOACl24, BOACl35, BOABr24, BOABr35, BTAF24, BTACl24 and BTABr24 without traditional gelation groups could form organogels. It was found that introduction of chlorine atoms in the 2,4-positions of the phenyl group would improve gelation abilities, and benzothiazole derivatives exhibited better gelation abilities than benzoxazoles with a similar π-skeleton due to better π-electron delocalization. Interestingly, the organogel of BTACl24 could change into solution by UV light due to trans-cis isomerization, which could also induce morphological changes in xerogels. The smooth organogel nanofibers stretched out lots of thin 'arms' to hold together or to catch other nanofibers upon UV irradiation, so more entangled networks were generated. Moreover, TFA (trifluoroacetic acid) could induce a gel-sol transformation on account of the protonation of the benzoxazole or benzothiazole unit, accompanied by emission quenching. BTACl24 exhibited higher performance than BOACl24 in the detection of TFA because of its strong basicity. The decay time and the detection limit of BTACl24 in xerogel-based film towards TFA vapor were of 0.7 s and 0.3 ppm, respectively. Therefore, organogelation of non-traditional organogelators is a powerful approach to the fabrication of multi-stimuli-responsive soft materials, and provides a new method to generate more entangled 3D networks through photochemical reactions in xerogels.
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http://dx.doi.org/10.1039/c8ob00113hDOI Listing
March 2018

Novel Wearable Electrodes Based on Conductive Chitosan Fabrics and Their Application in Smart Garments.

Materials (Basel) 2018 Mar 2;11(3). Epub 2018 Mar 2.

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.

Smart garments, which can capture electrocardiogram signals at any time or location, can alert others to the risk of heart attacks and prevent sudden cardiac death when people are sleeping, walking, or running. Novel wearable electrodes for smart garments based on conductive chitosan fabrics were fabricated by electroless plating of silver nanoparticles onto the surfaces of the fibers. The electrical resistance, which is related to the silver content of the composite fabrics, can be as low as 0.0332 ± 0.0041 Ω/sq due to the strong reactivity between amine groups and silver ions. After washing these fabrics eight times, the electrical resistance remained below 1 Ω/sq. The conductive chitosan fabrics were applied to smart garments as wearable electrodes to capture electrocardiogram signals of the human body in static state, jogging state, and running state, which showed good data acquisition ability and sensitivity.
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http://dx.doi.org/10.3390/ma11030370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872949PMC
March 2018

Toward Practical, Subwavelength, Visible-Light Photolithography with Hyperlens.

ACS Nano 2018 01 2;12(1):542-548. Epub 2018 Jan 2.

Electrical Engineering Department, University at Buffalo, The State University of New York , Buffalo, New York 14260, United States.

The future success of semiconductor technology relies on the continuing reduction of the feature size, allowing more components per chip and higher speed. Optical metamaterial-based hyperlens exhibit the ability for spatial pattern compression from the micro- to nanoscale, potentially addressing the ever-increasing demand of photolithograpy for inexpensive, all-optical nanoscale pattern recoding. Here, we demonstrate a photolithography system enabling a feature size of 80 nm using a 405 nm laser source. To realize such a system, we developed a fabrication method to obtain very thick hyperbolic metamaterial enabling a hyperlens with a very large demagnification rate of 3.75. Finally, we discuss several steps necessary to transform the proposed technique into a practical solution for the visible-light-based nanolithography. These include flattening of the inner surface of the hyperlens to increase the working area and integrating the proposed device into a conventional stepper system.
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http://dx.doi.org/10.1021/acsnano.7b07185DOI Listing
January 2018