Publications by authors named "Jing Zhong"

335 Publications

Equating the PHQ-9 and GAD-7 to the HADS depression and anxiety subscales in patients with major depressive disorder.

J Affect Disord 2022 May 19. Epub 2022 May 19.

School of Public Health, Southern Medical University, Guangzhou, Guangdong, People's Republic of China; Guangdong Mental Health Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, People's Republic of China. Electronic address:

Objective: The present study aimed to equate the 9-item Patient Health Questionnaire (PHQ-9) and 7-item Generalized Anxiety Disorder Scale (GAD-7) to the Hospital Anxiety and Depression Scale (HADS) depression and anxiety subscales (HADS-D,HADS-A) respectively in patients with major depressive disorder (MDD) and generate crosswalks of each raw score.

Methods: As it is a single group design that adopts common-person equating method, a total of 460 patients with MDD completed the PHQ-9, GAD-7 and HADS at the same time. Rasch analysis was used to filter out invalid participants, investigate the psychometric properties of test items and participants, link the PHQ-9 and HADS-D as well as GAD-7 and HADS-A, and produce conversion tables respectively. The differences between original scores and converted scores were analyzed to validate the crosswalks.

Result: 401 samples of depression part and 396 samples of anxiety part were left for final samples. Both the PHQ-9 / HADS-D and GAD-7 / HADS-A combined analysis adequately fit the unidimensional Rasch model, demonstrated acceptable reliability and item-person targeting and showed no disordering category. Slight differential item functioning across gender was found in item PHQ9 and item GAD6. The crosswalks were generated and verified to be validity.

Limitations: The results might be restricted to patients with MDD recruited in a single mental health center.

Conclusion: The PHQ-9, GAD-7 and HADS depression and anxiety subscales were successfully linked, producing conversion tables that could be used for directly converting raw score from one instrument to the other.
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http://dx.doi.org/10.1016/j.jad.2022.05.079DOI Listing
May 2022

Psychological impact and workload of COVID-19 on healthcare workers in China during the early time of the pandemic: A cross-sectional study.

Disaster Med Public Health Prep 2022 May 20:1-21. Epub 2022 May 20.

Department of Anesthesiology, Zhongshan Hospital Fudan University, Shanghai 200032, China.

Objective: This study aimed to investigate the organization, workload, and psychological impact of COVID-19 on healthcare workers from the domestic Medical Aid Teams (MATs) sent to Wuhan in China.

Methods: MATs leaders and members involved in the care for COVID-19 patients were invited to participate in a study by completing two separate self-report questionnaires from April 1 to 24, 2020.

Results: Nine MAT leaders were involved and 464 valid questionnaires were collected from 140 doctors and 324 nurses. Mean age of the doctors and nurses were 39.34±6.70 (26∼58 yr) and 31.88±5.29 (21∼52 yr), with 72 (15.5%) being males. Nurse was identified as an independent risk factor (HR 1.898; p=0.001) for a day working time in the multivariate analysis. The proportions of psychological consulting received among nurses were higher than those among doctors (49.7vs30.0%, p<0.001). More than half of the anesthetists and emergency doctors who have received psychological consulting thought that it was effective according to self-evaluation.

Conclusions: This study focused on healthcare workers' situation during the early time of pandemic. Nurse worked longer than doctors. The effectiveness of psychological consulting depends on the physicians' specialties. Working conditions of nurses and psychological consulting targeting different specialties need to be improved.
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http://dx.doi.org/10.1017/dmp.2022.129DOI Listing
May 2022

Automated Development of an Accurate Diffusion Database in Fcc AlCoCrFeNi High-Entropy Alloys from a Big Dataset of Composition Profiles.

Materials (Basel) 2022 Apr 30;15(9). Epub 2022 Apr 30.

State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China.

This study aims to incorporate a big dataset of composition profiles of fcc AlCoCrFeNi alloys, in addition to those of the related subsystem, to develop a self-consistent kinetic description for quinary high-entropy alloys. The latest feature of the HitDIC (gh-hroughput etermination of nterdiffusion oefficients) code was adopted in a high-throughput and automatic manner for accommodating a dataset of composition profiles with up to 87 diffusion couples. A good convergence for the optimization process was achieved, while satisfactory results regarding the composition profiles and previously evaluated diffusion properties were obtained. Here, we present an investigation into the elemental effect of Al towards interdiffusion and tracer diffusion, and their potential effect on creep and precipitation processes.
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http://dx.doi.org/10.3390/ma15093240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9102832PMC
April 2022

Mucinous Tubular and Spindle Cell Carcinoma of the Kidney: A Study of Clinical, Imaging Features and Treatment Outcomes.

Front Oncol 2022 11;12:865263. Epub 2022 Apr 11.

Department of Urology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.

Purpose: To describe the clinical, imaging, pathological features and oncologic outcomes of mucinous tubular and spindle cell carcinoma (MTSCC) of the kidney.

Patients And Methods: Twenty-two cases of MTSCC were pathologically identified between January 2004 and April 2021 at our institution. The clinical and imaging findings, pathological features, treatment methods and outcomes of the patients were reviewed.

Results: These cases included 17 women and 5 men, with a median age at diagnosis of 52.5 years. On contrast-enhanced CT, MTSCC was less enhanced than the adjacent renal parenchyma. Tumor attenuation values were 33.3 ± 6.8HU, 44.0 ± 9.1HU, 54.4 ± 13.9HU and 67.1 ± 11.8HU in the non-contrast, corticomedullary, nephrographic and excretory phases of CT, respectively. Contrast-enhanced ultrasonography and MRI also showed hypovascular features of the masses. On MRI, the tumors were isointense on T1-weighted images and slightly hypo- or hyperintense on T2-weighted images. Diffusion-weighted imaging revealed a low apparent diffusion coefficient of the tumor. The patients were managed with laparoscopic partial nephrectomy (n=5), radical nephrectomy (n=16), or robotic-assisted laparoscopic partial nephrectomy (n=1). The median follow-up time was 59.5 months. All the patients were free of local recurrence or distant metastasis.

Conclusions: MTSCC is generally indolent and has favorable outcomes. The imaging features of MTSCC are generally hypovascular, which is significantly different from clear cell renal cell carcinoma. However, it is still difficult to distinguish MTSCC from other hypovascular renal tumors preoperatively because their imaging features overlap. Further studies are essential to fully characterize the features of this rare RCC variant.
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http://dx.doi.org/10.3389/fonc.2022.865263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035933PMC
April 2022

An experimental investigation of particle and NOx emissions for a non-road diesel engine equipped with an integrated DOC + CDPF + SCR aftertreatment system during different operations.

Environ Sci Pollut Res Int 2022 Apr 25. Epub 2022 Apr 25.

Center of Research and Department, Kailong High Technology Company Limited, Wuxi, 214153, China.

An integrated aftertreatment system consisting of diesel oxidation catalyst (DOC), catalytic diesel particulate filter (CDPF), and selective catalytic reduction (SCR) is an effective way of reducing both NOx and particulate matter (PM). In this paper, the effect of DOC + CDPF + SCR on NO and particle emissions is investigated during different operations to assess applicability of this aftertreatment for meeting more rigorous non-road emissions standard. Meanwhile non-negligible issue about regeneration emission is studied. The results show that the DOC + CDPF have no significant effect on NOx but increase the NO/NOx ratio which is correlated with load. SCR is the main NOx reduction device with average efficiency of 86.5% for steady-state operations. Meanwhile, NH slip is lower than 16 ppm. During cold and hot non-road transient cycles (NRTC cycles), average NOx efficiencies are 56.7% and 57.8%, respectively, along with NH slip below 10 ppm. DOC + CDPF + SCR maintain filtration efficiency over 97% and 99% for PM and particle number (PN) for either steady-state operation or NRTC cycle, but particle size distributions change. Compared with the original emissions, NOx, PM, and PN emission factors are all below non-road China-IV limit after equipping with DOC + CDPF + SCR. However, during regeneration the aftertreatment does not maintain a high filtration performance but becomes particle generator. The penetration of nuclear particles and decomposition of agglomerated particles lead to high CDPF-out PN of 1.5 × 10 #/cm-3.5 × 10 #/cm. During regeneration, accumulated NOx is negligible, but the PM is 121.6 and 44.5 times higher than cold and hot NRTC cycles, respectively. In summary, DOC + CDPF + SCR is excellent way to improve non-road emissions but low SCR efficiency at low-temperature and high accumulated PM during regeneration process should be further addressed.
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http://dx.doi.org/10.1007/s11356-022-20152-xDOI Listing
April 2022

Wnt signaling in triple-negative breast cancers: Its roles in molecular subtyping and cancer cell stemness and its crosstalk with non-coding RNAs.

Life Sci 2022 Jul 21;300:120565. Epub 2022 Apr 21.

Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR China; Institute of Clinical Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China. Electronic address:

Triple-negative breast cancers (TNBCs) are now acknowledged as a collection of diseases encompassing distinct histological plasticity, multi-tier molecular heterogeneity, as well as different outcomes. Despite decades of efforts, the molecular subtyping strategy has been theoretical, and target therapies based on molecular alternations barely improve survival rates of TNBC patients, and thus traditional chemotherapy remains the standard of care in clinic. The Wnt signaling is an evolutionarily conserved signaling pathway, playing critical roles in embryogenesis and neoplastic disease. The dysregulation of Wnt signaling pathway endows cancer cells with stem cell-like capacities of self-renewal, cell proliferation and differentiation, thus exerting crucial roles in tumorigenesis and therapy responses. Recently, the gene expression assays and genomic sequencing have demonstrated that the dysregulation of Wnt signaling is associated with progression of TNBCs, particularly with metastasis, relapse and therapy resistance. In this review, we highlight the dysregulation of Wnt signaling in TNBCs and its potential biological roles in molecular subtyping and stemness traits of specific subtypes, as well as its crosstalk with ncRNAs in regulation of the biological features of TNBCs, aiming to update this important oncogenic signaling pathway in TNBCs.
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http://dx.doi.org/10.1016/j.lfs.2022.120565DOI Listing
July 2022

A Chinese adaptation of six items, self-report Hamilton Depression Scale: Factor structure and psychometric properties.

Asian J Psychiatr 2022 Apr 6;73:103104. Epub 2022 Apr 6.

Guangdong Mental Health Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, People's Republic of China. Electronic address:

Objectives: The objective of this research was to verify the psychometric characteristics of the Chinese Adaptation of self-report HAMD-6.

Methods: Outpatients and inpatients who met the DSM-5 criterion for major depressive disorder (MDD) were evaluated by the Chinese self-report HAMD-6, seventeen items of Hamilton Depression Rating Scale (HAMD-17), Patient Health Questionnaire Depression Scale (PHQ-9) and Improved Clinical Global Impression Scale (iCGI-S). The internal consistency reliability, retest reliability, criterion validity and construct validity of the Chinese self-report HAMD-6 were tested. Pearson correlation coefficient was used to assess the correlativity between the total score and the item scores. By drawing the Receiver Operating Characteristics (ROC) curve, the best cut-off value, sensitivity and specificity of Chinese Adaptation self-report HAMD-6 were obtained.

Results: Cronbach's alpha coefficient of the Chinese self-report HAMD-6 was 0.91, and the intra-group correlation coefficient (ICC) of retest reliability was 0.81(P < 0.01). The Spearman correlation coefficients of the Chinese self-report HAMD-6, Chinese clinician version of HAMD-6, PHQ-9 and HAMD-17 were 0.86, 0.81 and 0.86, respectively (all P < 0.01). Results of the confirmatory factor analysis (CFA) supported a unidimensional construct. In addition, HAMD-17 ≤ 7 and iCGI-S= 1 were taken as the remission criteria for depression disorder, and the ROC curves of the Chinese self-report HAMD-6 were plotted with a cut-off value of 3/4, the specificity and sensitivity were 0.85/0.92 and 0.96/0.93 respectively.

Conclusion: These results suggested that the abbreviated Chinese self-report HAMD-6 has good reliability and validity among the Chinese population. This study suggested that the remission cut-off value of the scale is 3/4.
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http://dx.doi.org/10.1016/j.ajp.2022.103104DOI Listing
April 2022

Effects of Perceptual Learning on Deprivation Amblyopia in Children with Limbal Dermoid: A Randomized Controlled Trial.

J Clin Med 2022 Mar 28;11(7). Epub 2022 Mar 28.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Sun Yat-Sen University, Guangzhou 510060, China.

Limbal dermoid (LD) is a congenital ocular tumor that causes amblyopia and damages visual acuity (VA) and visual function. This study evaluated the therapeutic efficacy of perceptual learning (PL) toward improving contrast sensitivity function (CSF) and VA. A total of 25 children with LD and 25 normal children were compared in terms of CSF and VA. The LD group was further randomly allocated into two arms: nine underwent PL combined with patching and eight underwent patching only; eight patients quit the amblyopia treatment. The primary outcome was the area under log CSF (AULCSF), and the secondary outcome was the best corrected VA (BCVA). The CSF was obviously reduced in the LD group compared with that in the normal group. Moreover, the difference in the changes in the AULCSF between the PL and patching groups after 6 months of training was 0.59 (95% CI: 0.32, 0.86, < 0.001), and the between-group difference in VA at 6 months was -0.30 (95% CI: -0.46, -0.14, < 0.001). Children suffering from LD with amblyopia exhibited CSF deficits and VA loss simultaneously. PL could improve CSF and VA in the amblyopic eye better than patching.
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http://dx.doi.org/10.3390/jcm11071879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999262PMC
March 2022

Investigation of the Effect of Graphene Oxide on the Properties and Microstructure of Clay-Cement Composite Grouting Materials.

Materials (Basel) 2022 Feb 22;15(5). Epub 2022 Feb 22.

School of Civil Engineering, Harbin Institute of Technology, Harbin 150006, China.

Reductions in bleeding rates and bulk shrinkage of grouting repair materials comprise the key to solving the leakage of earth-rock dams. In this paper, an anti-seepage grouting material for earth-rock dam was developed by introducing mineral admixtures and graphene oxide (GO) nano sheets into low-cost clay-cement grouting materials and by adding polycarboxylate superplasticizers (PCs) to improve slurry viscosity. The experimental results show that the shear stress and viscosity of the slurry increase with the increase in GO concentration, and the slurry has a certain thixotropy. GO can provide a platform to promote the formation of hydration products and fill the pores of clay particles due to its high specific surface area and low volume; in this paper, the microstructure of clay-cement-graphene oxide (CCGO) grouting materials were improved. Therefore, the bleeding rate, bulk shrinkage rate, setting time and unconfined compressive strength (UCS) of the sample were macroscopically improved. In particular, the bleeding rate and bulk shrinkage rate were shown to be 0% when the content of GO reached 1.08 g/kg. Thus, the grouting anti-seepage and reinforcement performance of CCGO grouting materials were improved.
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http://dx.doi.org/10.3390/ma15051623DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910877PMC
February 2022

3D Printing Graphene Oxide Soft Robotics.

ACS Nano 2022 Mar 15;16(3):3664-3673. Epub 2022 Feb 15.

Key Laboratory of Advanced Structural-Functional Integration Materials & Green Manufacturing Technology, Harbin Institute of Technology, Harbin 150001, China.

We propose a universal strategy to 3D printing the graphene oxide (GO) complex structure with GO highly aligned and densely compacted, by the combination of direct ink writing and constrained drying. The constraints not only allow the generation of a huge capillary force accompanied by water evaporation at nanoscale, which induces the high compaction and alignment of GO, but also limit the shrinkage of the extruded filaments only along the wall thickness direction, therefore, successfully maintaining the uniformity of the structure at macroscale. We discover that the shrinkage stress gradually increased during the drying process, with the maximum exceeding ∼0.74 MPa, significantly higher than other colloidal systems. Interestingly, because of the convergence between plates with different orientations of the constraints, a gradient of porosity naturally formed across the thickness direction at the corner. This allows us to 3D print humidity sensitive GO based soft robotics.
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http://dx.doi.org/10.1021/acsnano.1c06823DOI Listing
March 2022

Selective Activation of Unstrained C(O)-C Bond in Ketone Suzuki-Miyaura Coupling Reaction Enabled by Hydride-Transfer Strategy.

Org Lett 2022 02 7;24(6):1372-1377. Epub 2022 Feb 7.

College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China.

A Rh(I)-catalyzed ketone Suzuki-Miyaura coupling reaction of benzylacetone with arylboronic acid is developed. Selective C(O)-C bond activation, which employs aminopyridine as a temporary directing group and ethyl vinyl ketone as a hydride acceptor, occurs on the alkyl chain containing a β-position hydrogen. A series of acetophenone products were obtained in yields up to 75%.
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http://dx.doi.org/10.1021/acs.orglett.2c00110DOI Listing
February 2022

Segmentation of HE-stained meningioma pathological images based on pseudo-labels.

PLoS One 2022 4;17(2):e0263006. Epub 2022 Feb 4.

College of Computer and Cyber Security, Fujian Normal University, Fuzhou, Fujian, China.

Biomedical research is inseparable from the analysis of various histopathological images, and hematoxylin-eosin (HE)-stained images are one of the most basic and widely used types. However, at present, machine learning based approaches of the analysis of this kind of images are highly relied on manual labeling of images for training. Fully automated processing of HE-stained images remains a challenging task due to the high degree of color intensity, size and shape uncertainty of the stained cells. For this problem, we propose a fully automatic pixel-wise semantic segmentation method based on pseudo-labels, which concerns to significantly reduce the manual cell sketching and labeling work before machine learning, and guarantees the accuracy of segmentation. First, we collect reliable training samples in a unsupervised manner based on K-means clustering results; second, we use full mixup strategy to enhance the training images and to obtain the U-Net model for the nuclei segmentation from the background. The experimental results based on the meningioma pathology image dataset show that the proposed method has good performance and the pathological features obtained statistically based on the segmentation results can be used to assist in the clinical grading of meningiomas. Compared with other machine learning strategies, it can provide a reliable reference for clinical research more effectively.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0263006PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815980PMC
March 2022

Insights into N6-methyladenosine and programmed cell death in cancer.

Mol Cancer 2022 01 28;21(1):32. Epub 2022 Jan 28.

Department of Clinical Laboratory, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen, 518033, Guangdong, China.

N6-methyladenosine (m6A) methylation, the most common form of internal RNA modification in eukaryotes, has gained increasing attention and become a hot research topic in recent years. M6A plays multifunctional roles in normal and abnormal biological processes, and its role may vary greatly depending on the position of the m6A motif. Programmed cell death (PCD) includes apoptosis, autophagy, pyroptosis, necroptosis and ferroptosis, most of which involve the breakdown of the plasma membrane. Based on the implications of m6A methylation on PCD, the regulators and functional roles of m6A methylation were comprehensively studied and reported. In this review, we focus on the high-complexity links between m6A and different types of PCD pathways, which are then closely associated with the initiation, progression and resistance of cancer. Herein, clarifying the relationship between m6A and PCD is of great significance to provide novel strategies for cancer treatment, and has a great potential prospect of clinical application.
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http://dx.doi.org/10.1186/s12943-022-01508-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796496PMC
January 2022

Horner Syndrome Following Intercostal Nerve Block Via an Anterolateral Approach in Breast Lumpectomy: A Prospective Nested Case-control Study.

Pain Physician 2022 Jan;25(1):E55-E65

Department of Anesthesiology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China; Department of Anesthesiology, Zhongshan Hospital Fudan University, Shanghai, China; Department of Anesthesiology, Zhongshan Wusong Hospital Affiliated to Fudan University, Shanghai, China; Fudan Zhangjiang Institute, Shanghai, China; Shanghai Key Laboratory of Perioperative Stress and Protection.

Background: Lumpectomy is important for preventing malignant changes in benign tumors and diagnosing malignant tumors. Intercostal nerve blocks (ICNBs) are useful for breast lumpectomy as either the primary anesthetic or as an adjuvant anesthetic procedure. To our knowledge, no studies have evaluated the association between Horner syndrome and ICNB.

Objectives: This study aimed to explore the characteristics of and related risk factors for Horner syndrome after ICNB.

Study Design: A prospective, nested case-control study.

Setting: Fudan University Shanghai Cancer Centre from April 2020 through July 2020.

Methods: Patients scheduled for breast lumpectomy under ICNB from April 2020 through July 2020 in our hospital were recruited. The ICNB was introduced at the intersection of the midaxillary line and the inferior border of the ribs, according to the location of the mass. Horner syndrome indicators were assessed one, 5, 10, 15, 30, 45, and 60 minutes and 3, 6, 12 and 24 hours after ICNB. Personal data (age, body mass index [BMI], ASA classes), data on anesthetic (the puncture points, dose of local anesthetics, duration of ICNB, Horner syndrome indicators, other complications) and data on postoperative recovery (postoperative activity time, postoperative feeding time) were recorded. Univariate and multivariate logistic regression was used to estimate adjusted odds ratios and 95% confidence intervals.

Results: Ipsilateral Horner syndrome was found in 35 of 998 (3.5%) patients. Ipsilateral miosis, the first symptom to appear and last to disappear, occurred within 4 minutes and lasted 45 minutes to 240 minutes after ICNB. Seven patients showed obvious ipsilateral facial flushing. Logistic multivariate regression analysis showed that independent risk factors for Horner syndrome after ICNB were age <= 45 years, body mass index <= 18.5 kg/m2, and the need for a second ICNB.

Limitations: Firstly, the patients in this study are all adult women, and the applicability of other populations is uncertain. Secondly, the flow trajectory of local anesthetics was not confirmed by imaging tracers.

Conclusions: ICNB via an anterolateral approach promoted enhanced recovery after breast lumpectomy. The incidence of Horner syndrome following ICNB for breast lumpectomy was 3.5%. Horner syndrome occurred on the ipsilateral side of the ICNB and was reversible. Younger age, lower BMI, and the need for a second ICNB were risk factors for Horner syndrome after ICNB.

Key Words: Horner's syndrome, intercostal nerve block, breast lumpectomy, enhanced recovery.
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January 2022

Integrated hepatic single-cell RNA sequencing and untargeted metabolomics reveals the immune and metabolic modulation of Qing-Fei-Pai-Du decoction in mice with coronavirus-induced pneumonia.

Phytomedicine 2022 Mar 2;97:153922. Epub 2022 Jan 2.

School of Pharmacy, Second Military Medical University, Shanghai 200433, China; The Research Center for Traditional Chinese Medicine, Shanghai Institute of Infectious Diseases and Biosafety, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Background: Although Qing-Fei-Pai-Du decoction (QFPDD) is extensively used clinically to treat COVID-19 patients, the mechanism by which it modulates the immunological and metabolic functions of liver tissue remains unknown.

Purpose: The purpose of this study is to investigate the mechanism of action of QFPDD in the treatment of mice with coronavirus-induced pneumonia by combining integrated hepatic single-cell RNA sequencing and untargeted metabolomics.

Methods: We developed a human coronavirus pneumonia model in BALB/c mice by infecting them with human coronavirus HCoV-229E with stimulating them with cold-damp environment. We initially assessed the status of inflammation and immunity in model mice treated with or without QFPDD by detecting peripheral blood lymphocytes and inflammatory cytokines. Then, single-cell RNA sequencing and untargeted metabolomics were performed on mouse liver tissue.

Results: HCoV-229E infection in combination with exposure to a cold-damp environment significantly decreased the percentage of peripheral blood lymphocytes (CD4 and CD8 T cells, B cells) in mice, which was enhanced by QFPDD therapy. Meanwhile, the levels of inflammatory cytokines such as IL-6, TNF-α, and IFN-γ were significantly increased in mouse models but significantly decreased by QFPDD treatment. Single-cell RNA sequencing analysis showed that QFPDD could attenuate disease-associated alterations in gene expression, core transcriptional regulatory networks, and cell-type composition. Computational predictions indicated that QFPDD rectified the observed aberrant patterns of cell-cell communication. Additionally, the metabolic profiles of liver tissue in the Model group were distinct from mice in the Control group, and QFPDD significantly regulated hepatic purine metabolism.

Conclusion: To the best of our knowledge, this study is the first to integrate hepatic single-cell RNA sequencing and untargeted metabolomics into a TCM formula and these valuable findings indicate that QFPDD can improve immune function and reduce liver injury and inflammation.
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http://dx.doi.org/10.1016/j.phymed.2021.153922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720382PMC
March 2022

Ubiquitination regulation of aerobic glycolysis in cancer.

Life Sci 2022 Mar 11;292:120322. Epub 2022 Jan 11.

Institute of Clinical Medicine, the First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR China; Cancer Research Institute, the First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR China. Electronic address:

Aerobic glycolysis, or the Warburg effect, is regarded as a critical part of metabolic reprogramming and plays a crucial role in the occurrence and development of tumours. Ubiquitination and deubiquitination, essential post-translational modifications, have attracted increasing attention with regards to the regulation of metabolic reprogramming in cancer. However, the mechanism of ubiquitination in glycolysis remains unclear. In this review, we discuss the roles of ubiquitination and deubiquitination in regulating glycolysis, and their involvement in regulating important signalling pathways, enzymes, and transcription factors. Focusing on potential mechanisms may provide novel strategies for cancer treatment.
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http://dx.doi.org/10.1016/j.lfs.2022.120322DOI Listing
March 2022

An Effective Strategy to Maintain the CALPHAD Atomic Mobility Database of Multicomponent Systems and Its Application to Hcp Mg-Al-Zn-Sn Alloys.

Materials (Basel) 2021 Dec 31;15(1). Epub 2021 Dec 31.

State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China.

In this paper, a general and effective strategy was first developed to maintain the CALPHAD atomic mobility database of multicomponent systems, based on the pragmatic numerical method and freely accessible HitDIC software, and then applied to update the atomic mobility descriptions of the hcp Mg-Al-Zn, Mg-Al-Sn, and Mg-Al-Zn-Sn systems. A set of the self-consistent atomic mobility database of the hcp Mg-Al-Zn-Sn system was established following the new strategy presented. A comprehensive comparison between the model-predicted composition-distance profiles/inter-diffusivities in the hcp Mg-Al-Zn, Mg-Al-Sn, and Mg-Al-Zn-Sn systems from the presently updated atomic mobilities and those from the previous ones that used the traditional method indicated that significant improvement can be achieved utilizing the new strategy, especially in the cases with sufficient experimental composition-distance profiles and/or in higher-order systems. Furthermore, it is anticipated that the proposed strategy can serve as a standard for maintaining the CALPHAD atomic mobility database in different multicomponent systems.
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http://dx.doi.org/10.3390/ma15010283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746026PMC
December 2021

Nb/Sn Liquid-Solid Reactive Diffusion Couples and Their Application to Determination of Phase Equilibria and Interdiffusion Coefficients of Nb-Sn Binary System.

Materials (Basel) 2021 Dec 24;15(1). Epub 2021 Dec 24.

State Key Lab of Powder Metallurgy, Central South University, Changsha 410083, China.

NbSn plays an irreplaceable role in superconducting parts due to its stable performance under high field conditions. Accurate phase equilibria and interdiffusion coefficients are of great significance for designing novel NbSn superconductors. However, the related experimental information is still in a state of scarcity because of the difficulty in fabrication of Nb-Sn alloys caused by the large difference in melting points of Nb and Sn. In this paper, a simple but pragmatic approach was first proposed to prepare the Nb/Sn liquid-solid reactive diffusion couples (LSDCs) at 1100 °C and 1200 °C, of which the phase identification of the formed layer and the measurement of composition-distance profiles were conducted. The formed layer in Nb/Sn LSDCs was confirmed to be NbSn compound. While the measured composition profiles were employed to determine the phase equilibria according to the local equilibrium hypothesis and the interdiffusion coefficients with an aid of the latest version of HitDIC software. The determined phase equilibria of NbSn, (Nb) and liquid show good agreement with the assessed phase diagram. While the calculated interdiffusion coefficients and activation energy for diffusion in NbSn are consistent with both experimental and theoretical data in the literature. Moreover, the growth of the formed NbSn layer in Nb/Sn LSDCs was also found to be diffusion controlled. All the obtained phase equilibria and interdiffusion coefficients are of great value for further thermodynamic and kinetic modeling of the Nb-Sn system. Furthermore, it is anticipated that the presently proposed approach of fabricating liquid-solid reactive diffusion couple should serve as a general one for various alloy systems with large differences in melting points.
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http://dx.doi.org/10.3390/ma15010113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746297PMC
December 2021

Exploring the Mechanism of Saponins against Alzheimer's Disease by Network Pharmacology and Experimental Validation.

Evid Based Complement Alternat Med 2021 30;2021:5730812. Epub 2021 Dec 30.

School of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China.

Background: saponins (PNS) have been used for neurodegenerative disorders such as cerebral ischemia and Alzheimer's disease (AD). Although increasing evidences show the neuron protective effects of PNS, the vital compounds and their functional targets remain elusive. To explore the potential functional ingredients of PNS for the AD treatment and their molecular mechanisms, an neuron injured model induced by A was investigated, and the potential mechanism was predicted by network pharmacology approach and validated by molecular biology methods.

Methods: Network pharmacology approach was used to reveal the relationship between ingredient-target disease and function-pathway of PNS on the treatment of AD. The active ingredients of PNS were collected from TCMSP, PubChem database, and literature mining in PubMed database. DrugBank and GeneCards database were used to predict potential targets for AD. The STRING database was performed to reveal enrichment of these target proteins, protein-protein interactions, and related pathways. Networks were visualized by utilizing Cytoscape software. The enrichment analysis was performed by the DAVID database. Finally, neuroprotective effect and predictive mechanism of PNS were investigated in an AD model established by A -treated PC12 cells.

Results: An ingredient-target disease and function-pathway network demonstrated that 38 active ingredients were derived from PNS modulated 364 common targets shared by PNS and AD. GO and KEGG analysis, further clustering analysis, showed that mTOR signaling targets were associated with the neuroprotective effects of PNS. In A-treated PC12 cells, PNS treatment improved neuroprotective effect, including mTOR inhibition and autophagy activation.

Conclusions: Collectively, the protective effects of PNS on AD-neuron injury are related to the inhibition of mTOR and autophagy activation.
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http://dx.doi.org/10.1155/2021/5730812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739172PMC
December 2021

Shape Optimization of Costal Cartilage Framework Fabrication Based on Finite Element Analysis for Reducing Incidence of Auricular Reconstruction Complications.

Front Bioeng Biotechnol 2021 13;9:766599. Epub 2021 Dec 13.

Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Skin necrosis is the most common complication in total auricular reconstruction, which is mainly induced by vascular compromise and local stress concentration of the overlying skin. Previous studies generally emphasized the increase in the skin flap blood supply, while few reports considered the mechanical factors. However, skin injury is inevitable due to uneasily altered loads generated by the intraoperative continuous negative suction and uneven cartilage framework structure. Herein, this study aims to attain the stable design protocol of the ear cartilage framework to decrease mechanical damage and the incidence of skin necrosis. Finite element analysis was initially utilized to simulate the reconstructive process while the shape optimization technique was then adopted to optimize the three-pretested shape of the hollows inside the scapha and fossa triangularis under negative suction pressure. Finally, the optimal results would be output automatically to meet clinical requirement. Guided by the results of FE-based shape optimization, the optimum framework with the smallest holes inside the scapha and fossa triangularis was derived. Subsequent finite element analysis results also demonstrated the displacement and stress of the post-optimized model were declined 64.9 and 40.1%, respectively. The following clinical study was performed to reveal that this new design reported lower rates of skin necrosis decrease to 5.08%, as well as the cartilage disclosure decreased sharply from 14.2 to 3.39% compared to the conventional method. Both the biomechanical analysis and the clinical study confirmed that the novel design framework could effectively reduce the rates of skin necrosis, which shows important clinical significance for protecting against skin necrosis.
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http://dx.doi.org/10.3389/fbioe.2021.766599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8711272PMC
December 2021

Development and Validation of a Nomogram for Predicting Sarcopenia in Community-Dwelling Older Adults.

J Am Med Dir Assoc 2022 May 20;23(5):715-721.e5. Epub 2021 Dec 20.

Xiangya Nursing School, The Central South University, Changsha, China. Electronic address:

Objective: To establish and validate a nomogram that predicts the risk of sarcopenia for community-dwelling older residents.

Design: Retrospective study.

Setting And Participants: A total of 1050 community-dwelling older adults.

Methods: Data from a survey of community-dwelling older residents (≥60 years old) in Hunan, China, from June to September 2019 were retrospectively analyzed. The survey included general demographic information, diet, and exercise habits. Sarcopenia diagnosis was according to 2019 Asian Working Group for Sarcopenia criteria. Participants were randomly divided into the development group and validation groups. Independent risk factors were screened by multivariate logistic regression analysis. Based on the independent risk factors, a nomogram model was developed to predict the risk of sarcopenia for community-dwelling older adults. Both in the development and validation sets, the discrimination, calibration, and clinical practicability of the nomogram were verified using receiver operating characteristic curve analysis, Hosmer-Lemeshow test, and decision curve analysis, respectively.

Results: Sarcopenia was identified in 263 (25.0%) participants. Age, body mass index, marital status, regular physical activity habit, uninterrupted sedentary time, and dietary diversity score were significant contributors to sarcopenia risk. A nomogram for predicting sarcopenia in community-dwelling older adults was developed using these factors. Receiver operating characteristic curve analysis showed that the area under the curve was 0.827 (95% CI 0.792-0.860) and 0.755 (95% CI 0.680-0.837) in the development and validation sets, respectively. The Hosmer-Lemeshow test yielded P values of .609 and .565, respectively, for the 2 sets. The nomogram demonstrated a high net benefit in the clinical decision curve in both sets.

Conclusions And Implications: This study developed and validated a risk prediction nomogram for sarcopenia among community-dwelling older adults. Sarcopenia risk was classified as low (<11%), moderate (11%-70%), and high (>70%). This nomogram provides an accurate visual tool to medical staff, caregivers, and older adults for prediction, early intervention, and graded management of sarcopenia.
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http://dx.doi.org/10.1016/j.jamda.2021.11.023DOI Listing
May 2022

Exosomes in triple negative breast cancer: From bench to bedside.

Cancer Lett 2022 02 10;527:1-9. Epub 2021 Dec 10.

Institute of Clinical Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR China; Cancer Research Institute, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR China. Electronic address:

Exosomes are lipid bilayer extracellular vesicles with a size of 30-150 nm, which can be released by various types of cells including breast cancer cells. Exosomes are enriched with multiple nucleic acids, lipids, proteins and play critical biological roles by binding to recipient cells and transmitting various biological cargos. Studies have reported that tumor-derived exosomes are involved in cancer initiation and progression, such as promoting cancer invasion and metastasis, accelerating angiogenesis, contributing to epithelial-mesenchymal transition, and enhancing drug resistance in tumors. Recently the dysregulating of exosomes has been found in triple-negative breast cancer (TNBC), relating to the clinicopathological characteristics and prognosis of TNBC patients. Considering the poor prognosis and lack of adequate response to conventional therapy of TNBC, the discovery of certain exosomes as a new target for diagnosis and treatment of TNBC may be a good choice that provides new opportunities for the early diagnosis, clinical treatment of TNBC. Here, we first discuss the innovative prognostic and predictive effects of exosomes on TNBC, as well as the practical clinical problems. Secondly, we focus on the new therapeutic areas represented by exosomes, especially the impact of introducing exosomes in TNBC treatment in the future.
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http://dx.doi.org/10.1016/j.canlet.2021.12.009DOI Listing
February 2022

The roles and mechanisms of the m6A reader protein YTHDF1 in tumor biology and human diseases.

Mol Ther Nucleic Acids 2021 Dec 4;26:1270-1279. Epub 2021 Nov 4.

Hengyang Medical School, University of South China, 421001 Hengyang, Hunan, China.

YTHDF1 is the most versatile and powerful reader protein of -methyladenosine (m6A)-modified RNA, and it can recognize both G(m6A)C and A(m6A)C RNAs as ligands without sequence selectivity. YTHDF1 regulates target gene expression by different mechanisms, such as promoting translation or regulating the stability of mRNA. Numerous studies have shown that YTHDF1 plays an important role in tumor biology and nontumor lesions by mediating the protein translation of important genes or by affecting the expression of key factors involved in many important cell signaling pathways. Therefore, in this review we focus on some of the roles of YTHDF1 in tumor biology and diseases.
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http://dx.doi.org/10.1016/j.omtn.2021.10.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8609105PMC
December 2021

Panax notoginseng saponins protect PC12 cells against Aβ induced injury via promoting parkin-mediated mitophagy.

J Ethnopharmacol 2022 Mar 21;285:114859. Epub 2021 Nov 21.

School of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, PR China; Key Laboratory of Pharmacology of Chinese Medicine, Guangxi University of Chinese Medicine, Nanning 530200, PR China; Key Laboratory of Guangxi Zhuang and Yao Medicine; Nanning 530200, PR China. Electronic address:

Ethnopharmacological Relevance: Panax notoginseng (Burk) F. H. Chen is a well-known traditional Chinese medicine with a long history and is widely used in the treatment of cerebrovascular disease. Panax notoginseng saponins (PNS) are the main active ingredients in Panax notoginseng (Burk) F. H. Chen, and its injection is used to treat nerve damage caused by cerebral ischemia and other conditions. PNS is thought to alleviate cognitive impairment in patients with Alzheimer's disease; however, its mechanism of action is unclear.

Aim Of The Study: We elucidated the role of PNS in attenuating cellular mitochondrial damage caused by amyloid β (Aβ) protein and in protecting cell viability from the perspective of regulating autophagy. By investigating the effects of PNS on the targets regulating mitophagy, we wanted to reveal the autophagy related mechanism by which PNS attenuated Aβ damage in neuronal cells.

Materials And Methods: The effect of PNS on the mitochondrial membrane potential of Aβ-injured PC12 cells was detected using flow cytometry, which reflected the alleviating effect of PNS on mitochondrial damage. Using mRFP-GFP-LC3-transfected PC12 cells, the effect of PNS on cellular autophagy flux was observed using laser confocal microscopy. Formation of the intracellular autophagosome was observed using transmission electron microscopy, which reflected the activation of autophagy by PNS. The siPINK1 lentivirus was used to silence the PINK1 gene in PC12 cells to obtain siPINK1-PC12 cells. The effects of PNS on the expression of the PINK1 gene and on the autophagy-related proteins LC3II/Ⅰ, p62, PINK1, parkin, NDP52, and OPTN were observed to reveal the possible targets of PNS in regulating autophagy.

Results: After PNS treatment, the viability of Aβ-injured PC12 cells improved and the mitochondrial membrane potential was restored. PNS treatment significantly enhanced the autophagy flux of damaged cells and increased the levels of LC3II/Ⅰ protein and decreased p62 protein, while significantly improving the structure and mitochondrial morphology of PC12 cells injured by Aβ. These changes led to more autophagosomes wrapping around the damaged mitochondria and promoting the depletion of OPTN, a mitophagy receptor. After silencing the PINK1 gene, PNS could not alter the PINK1 gene and protein levels, but could still increase LC3II/Ⅰ, decrease p62 and OPTN, and significantly increase the amount of parkin.

Conclusions: PNS could enhance the autophagic activity of cells, alleviate mitochondrial damage caused by Aβ injury, and protect the activity of PC12 cells. It is possible that enhanced autophagy was achieved by promoting the recruitment of parkin protein to the mitochondrial receptors in a non-PINK1-dependent manner.
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http://dx.doi.org/10.1016/j.jep.2021.114859DOI Listing
March 2022

Refractive Error and Eye Health: An Umbrella Review of Meta-Analyses.

Front Med (Lausanne) 2021 4;8:759767. Epub 2021 Nov 4.

Department of Ophthalmology, Peking University Third Hospital, Beijing, China.

To explore the associations between refractive errors and multiple eye health outcomes. This is an umbrella review based on systematic reviews with meta-analyses. In our study, refractive errors included myopia, hyperopia, astigmatism, and anisometropia. We reconducted the meta-analyses whose primary data were available in sufficient detail by random effect model. Heterogeneity was assessed by . The main outcomes included myopic macular degeneration (MMD), retinal detachment (RD), cataract, open-angle glaucoma (OAG), strabismus, age-related macular degeneration (AMD), and diabetic retinopathy (DR). Myopia was associated with increased risk of MMD (relative risk = 102.11, 95% CI 52.6-198.22), RD (3.45, 1.08-11.00), nuclear cataract (2.15, 1.53-3.03), posterior subcapsular (PSC) cataract (1.74, 1.41-2.15), OAG (1.95, 1.74-2.19), exotropia (5.23, 2.26-12.09), but decreased risk of DR (0.83, 0.66-1.04), and early AMD (0.80, 0.67-0.94). From mild-to-high myopia, the association strengthened for MMD, RD, nuclear cataract, PSC cataract, OAG, and DR. Hyperopia was associated with an increased risk of early AMD (1.09, 1.01-1.18) and esotropia (22.94, 10.20-51.62). Astigmatism and anisometropia were associated with increased risk of both exotropia and esotropia. Myopia, especially high myopia, demonstrated the highest risk for eye health outcomes, such as MMD, RD, OAG, nuclear and PSC cataracts, and exotropia. However, myopia was associated with a lower risk of early AMD and DR. Individuals with hyperopia are more likely to suffer early AMD and esotropia. Astigmatism and anisometropia predispose to strabismus. A lot of research studies on the mechanism of the associations are needed. https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=239744; identifier: 239744.
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http://dx.doi.org/10.3389/fmed.2021.759767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599990PMC
November 2021

PTP70-2, a novel polysaccharide from Polygala tenuifolia, prevents neuroinflammation and protects neurons by suppressing the TLR4-mediated MyD88/NF-κB signaling pathway.

Int J Biol Macromol 2022 Jan 19;194:546-555. Epub 2021 Nov 19.

Clinical Pharmacy of The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510006, China; School of Clinical Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address:

PTP70-2, a novel polysaccharide isolated from Polygala tenuifolia in our previous publication, exhibits potential anti-inflammatory effects. Here, we investigate the mechanisms underlying these effects and the neuroprotective activity of PTP70-2 in lipopolysaccharide (LPS)-damaged BV2 microglial cells and neuroinflammation-injured primary cortical neurons. The results suggest that PTP70-2 dramatically reduces the LPS-stimulated inflammatory cytokines overexpression, as well as down-regulates the levels of TLR4-, MyD88-, and NF-κB-related proteins. The effect of PTP70-2 in down-regulation of proinflammatory cytokines and downstream proteins implicated in MyD88 and NF-κB signaling is related to the TLR4 pathway. Furthermore, this effect is enhanced by the co-incubation of BV2 cells with PTP70-2 and TAK242, a TLR4 inhibitor, before exposure to LPS. Importantly, PTP70-2 prevents neuroinflammation-induced neurotoxicity by mitigating ROS overproduction and MMP dissipation. Overall, the PTP70-2's anti-neuroinflammation and neuroprotection are involved to the modulation of the TLR4-mediated MyD88/NF-κB signaling pathway.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.11.097DOI Listing
January 2022

Gas embolism under standard versus low pneumoperitoneum pressure during laparoscopic liver resection (GASES): study protocol for a randomized controlled trial.

Trials 2021 Nov 15;22(1):807. Epub 2021 Nov 15.

Department of Anesthesiology, Zhongshan Hospital Fudan University, Shanghai, China.

Background: Gas embolism induced by CO pneumoperitoneum is commonly identified as a risk factor for morbidity, especially cardiopulmonary morbidity, after laparoscopic liver resection (LLR) in adults. Increasing pneumoperitoneum pressure (PP) contributes to gas accumulation following laparoscopy. However, few studies have examined the effects of PP in the context of LLR. In LLR, the PP-central venous pressure (CVP) gradient is increased due to hepatic vein rupture, hepatic sinusoid exposure, and low CVP management, which together increase the risk of CO embolization. The aim of this study is to primarily determine the role of low PP (10 mmHg) on the incidence of severe gas embolism.

Methods: Adult participants (n = 140) undergoing elective LLR will be allocated to either a standard (15 mmHg) or low (10 mmHg) PP group. Anesthesia management, postoperative care, and other processes will be performed similarly in both groups. The occurrence of severe gas embolism, which is defined as gas embolism ≥ grade 3 according to the Schmandra microbubble method, will be detected by transesophageal echocardiography (TEE) and recorded as the primary outcome. The subjects will be followed up until discharge and followed up by telephone 1 and 3 months after surgery. Postoperative outcomes, such as the Post-Operative Quality of Recovery Scale, pain severity, and adverse events, will be assessed. Serum cardiac markers and inflammatory factors will also be assessed during the study period. The correlation between intraoperative inferior vena cava-collapsibility index (IVC-CI) under TEE and central venous pressure (CVP) will also be explored.

Discussion: This study is the first prospective randomized clinical trial to determine the effect of low versus standard PP on gas embolism using TEE during elective LLR. These findings will provide scientific and clinical evidence of the role of PP.

Trial Status: Protocol version: version 1 of 21-08-2020 TRIAL REGISTRATION: ChiCTR2000036396 ( http://www.chictr.org.cn ). Registered on 22 August 2020.
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http://dx.doi.org/10.1186/s13063-021-05678-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591437PMC
November 2021

sp. nov. Causes Leaf Spots of in China.

Plant Dis 2022 Apr 30;106(4):1105-1113. Epub 2022 Mar 30.

College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu 210037, China.

Gaertn. (Sapindaceae), or soapberry, is an important biodiesel tree in southern China. In recent years, leaf spot disease on soapberry has been observed frequently in a soapberry germplasm repository in Jianning County, Sanming City, Fujian province, China. The symptoms initially appeared as irregular, small, yellow spots, and the centers of the lesions became dark brown with time. Three fungal isolates from lesions were collected. Koch's postulates were performed, and their pathogenicity was confirmed. Morphologically, α-conidia from diseased tissues were single-celled, hyaline, smooth, clavate or ellipsoidal, and biguttulate, measuring 6.2 to 7.2 × 2.3 to 2.7 μm. In addition, the three isolates in this study developed three types (α, β, and γ) of conidia on potato dextrose agar, and their morphological characteristics matched those of . A phylogenetic analysis based on internal transcribed spacer, , , , and sequence data determined that the three isolates are a new species of . Based on both morphological and phylogenetic analyses, the causal fungus, sp. nov., was described and illustrated.
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http://dx.doi.org/10.1094/PDIS-04-21-0777-REDOI Listing
April 2022

Noncoding RNAs in triple negative breast cancer: Mechanisms for chemoresistance.

Cancer Lett 2021 12 30;523:100-110. Epub 2021 Sep 30.

Institute of Clinical Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR China; Cancer Research Institute, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, PR China. Electronic address:

Triple-negative breast cancer (TNBC) is the most aggressive subtype among breast cancers with high recurrence and this condition is partly due to chemoresistance. Therefore, fully understanding the mechanism of TNBC-resistance is the key to overcoming chemoresistance, which will be an effective strategy for TNBC therapy. Various potential mechanisms involved in the chemoresistance of TNBC have been investigated and indicated that noncoding RNAs (ncRNAs) especially microRNAs (miRNAs), long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) take part in most TNBC resistance. The ncRNA-induced chemoresistance process is involved in the alteration of many activities. here, we mainly summarize the mechanisms of ncRNAs in the chemoresistance of TNBC and discuss the potential clinical application of ncRNAs in the treatment of TNBC, indicating that targeting ncRNAs might be a promising strategy for resensitization to chemotherapies.
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http://dx.doi.org/10.1016/j.canlet.2021.09.038DOI Listing
December 2021

Quantitative analysis of morphological and functional features in Meibography for Meibomian Gland Dysfunction: Diagnosis and Grading.

EClinicalMedicine 2021 Oct 11;40:101132. Epub 2021 Sep 11.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Centre, Sun Yat-sen University, Guangzhou, Guangdong, China.

Background: To explore the performance of quantitative morphological and functional analysis in meibography images by an automatic meibomian glands (MGs) analyser in diagnosis and grading Meibomian Gland Dysfunction (MGD).

Methods: A cross-sectional study collected 256 subjects with symptoms related to dry eye and 56 healthy volunteers who underwent complete ocular surface examination was conducted between January 1, 2019, and December 31, 2020. The 256 symptomatic subjects were classified into MGD group (n = 195) and symptomatic non-MGD group (n = 61). An automatic MGs analyser was used to obtained multi-parametric measurements in meibography images including the MGs area ratio (GA), MGs diameter deformation index (DI), MGs tortuosity index (TI), and MGs signal index (SI). Adjusted odds ratios (ORs) of the multi-parametric measurements of MGs for MGD, and the area under the receiver operating characteristic (AUC-ROC) curves of multi-parametric measurements for MGD diagnosing and grading were conducted.

Findings: When consider age, sex, ocular surface condition together, the estimated ORs for DI was 1.62 (95% CI, 1.29-2.56), low-level SI was 24.34 (95% CI, 2.73-217.3), TI was 0.76(95% CI, 0.54-0.90), and GA was 0.86 (95% CI, 0.74-0.92) for MGD. The combination of DI-TI-GA-SI showed an AUC = 0.82 ( < 0.001) for discriminating MGD from symptomatic subjects. The DI had a higher AUC in identifying early-stage MGD (grade 1-2), while TI and GA had higher AUCs in moderate and advanced stages (grade 3-5). Merging DI-TI-GA showed the highest AUCs in distinguish MGD severities.

Interpretation: The MGs area ratio, diameter deformation, tortuosity and signal intensity could be considered promising biomarkers for MGD diagnosis and objective grading.

Funding: This work was supported by the Key-Area Research and Development Program of Guangdong Province (No. 2019B010152001), the National Natural Science Foundation of China under Grant (81901788) and Guangzhou Science and Technology Program (202002030412).
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http://dx.doi.org/10.1016/j.eclinm.2021.101132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435692PMC
October 2021
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