Publications by authors named "Jing Yuan"

1,486 Publications

  • Page 1 of 1

From encoding to retrieval: Change in level of unitization resolves debate about Unitization's effect on associative recognition.

Psychophysiology 2022 Aug 10:e14161. Epub 2022 Aug 10.

Beijing Key Laboratory of Learning and Cognition, Department of Psychology, Capital Normal University, Beijing, China.

Although it is widely accepted that familiarity could support associative recognition when the to-be-learn items are 'unitized' into a new representation, the effects of unitization on associative recognition and recollection remain much debated. The current study aimed to explain these debates by exploring when and how unitization benefits associative recognition using event-related potentials (ERPs). During the encoding phase, participants learned compound words and unrelated word pairs (i.e., High vs. Low level of unitization). At retrieval, the compound words were rearranged into new compound words (i.e., no-change) and unrelated word pairs (i.e., change). Similarly, the unrelated word pairs were rearranged into new unrelated word pairs (i.e., no-change) and compound words (i.e., change). Results showed that under the no-change condition, unitization did not affect associative recognition, nor its underlying processes. In contrast, under the change condition, unitization improved associative recognition by increasing both familiarity-related FN400 effect and recollection-related LPC effect. In addition, a planned comparison between the compound-change and unrelated-no change conditions-a common index for unitization effect in past studies-revealed that unitization could not only elicit significant FN400 effect, but also improve associative recognition by increasing LPC effect. Collectively, these results not only allowed to explain the current discrepancies in the literature concerning the effect of unitization on associative recognition, but also emphasized the importance of matching the level of unitization between the studied and rearranged word pairs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/psyp.14161DOI Listing
August 2022

Biomimetic material degradation for synergistic enhanced therapy by regulating endogenous energy metabolism imaging under hypothermia.

Nat Commun 2022 Aug 5;13(1):4567. Epub 2022 Aug 5.

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, P. R. China.

Inefficient tumour treatment approaches often cause fatal tumour metastases. Here, we report a biomimetic multifunctional nanoplatform explicitly engineered with a Co-based metal organic framework polydopamine heterostructure (MOF-PDA), anethole trithione (ADT), and a macrophage membrane. Co-MOF degradation in the tumour microenvironment releases Co, which results in the downregulation of HSP90 expression and the inhibition of cellular heat resistance, thereby improving the photothermal therapy effect of PDA. HS secretion after the enzymatic hydrolysis of ADT leads to high-concentration gas therapy. Moreover, ADT changes the balance between nicotinamide adenine dinucleotide/flavin adenine dinucleotide (NADH/FAD) during tumour glycolysis. ATP synthesis is limited by NADH consumption, which triggers a certain degree of tumour growth inhibition and results in starvation therapy. Potentiated 2D/3D autofluorescence imaging of NADH/FAD is also achieved in liquid nitrogen and employed to efficiently monitor tumour therapy. The developed biomimetic nanoplatform provides an approach to treat orthotopic tumours and inhibit metastasis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-32349-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355994PMC
August 2022

Early-onset diabetes involving three consecutive generations had different clinical features from age-matched type 2 diabetes without a family history in China.

Endocrine 2022 Aug 3. Epub 2022 Aug 3.

Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China.

Purpose: Early-onset, multigenerational diabetes is a heterogeneous disease, which is often simplistically classified as type 1 diabetes (T1D) or type 2 diabetes(T2D). However, its clinical and genetic characteristics have not been clearly elucidated. The aim of our study is to investigate the clinical features of early-onset diabetes involving three consecutive generations (eDia3) in a Chinese diabetes cohort.

Methods: Of 6470 type 2 diabetic patients, 105 were identified as eDia3 (1.6%). After a case-control match on age, we compared the clinical characteristics of 89 eDia3 patients with 89 early-onset T2D patients without a family history of diabetes (eDia0). WES was carried out in 89 patients with eDia3. We primarily focused on 14 known maturity-onset diabetes of the young (MODY) genes. Variants were predicted by ten tools (SIFT, PolyPhen2_HDIV, PolyPhen2_HVAR, LRT, Mutation Assessor, Mutation Taster, FATHMM, GERP++, PhyloP, and PhastCons). All suspected variants were then validated by Sanger sequencing and further investigated in the proband families.

Results: Compared to age-matched eDia0, eDia3 patients had a younger age at diagnosis (26.5 ± 5.8 vs. 29.4 ± 5.3 years, P = 0.001), lower body mass index (25.5 ± 3.9 vs. 27.4 ± 4.6 kg/m, P = 0.003), lower systolic blood pressure (120 ± 15 vs. 128 ± 18 mmHg, P = 0.003), and better metabolic profiles (including glucose and lipids). Of the 89 eDia3 patients, 10 (11.2%) carried likely pathogenic variants in genes (KLF11, GCK, ABCC8, PAX4, BLK and HNF1A) of MODY.

Conclusions: eDia3 patients had unique clinical features. Known MODY genes were not common causes in these patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12020-022-03144-2DOI Listing
August 2022

Is dietary choline intake related to dementia and Alzheimer's disease risk: results from the Framingham Heart Study.

Am J Clin Nutr 2022 Aug 2. Epub 2022 Aug 2.

Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA.

Background: The positive association of choline for cognition has been reported in both animal and human studies, yet the association of choline with the risk of incident dementia or Alzheimer's disease (AD) in humans is unclear.

Objectives: Our objective was to test the hypothesis that lower (higher) dietary choline intake is associated with increased (decreased) risk of incident dementia or AD.

Design: Data from the Framingham Heart Study (FHS) Offspring Cohort Exam 5 to Exam 9 were used. Participants were free of dementia and stroke with valid self-report 126-item Harvard food-frequency questionnaire (FFQ) at Exam 5. The intakes of total choline, its contributing compounds, and betaine were estimated based on a published nutrient database. The intakes were updated at each exam to represent the cumulative average intake across the five exams. The associations between dietary choline intake and incident dementia and AD were examined in the mixed effect Cox proportional hazard models, adjusting for covariates.

Results: A total of 3,224 participants (53.8% female, mean ± SD age 54.5 ± 9.7 year) were followed up for a mean ± SD of 16.1 ± 5.1 years (1991-2011). There were 247 incident dementia cases, of which 177 were AD. Dietary choline intake showed non-linear relationship with incident dementia and AD. After adjusting for covariates, low choline intake (defined as choline/100 ≤ 2.19 and choline/100 ≤ 2.15 in our sample) was significantly associated with incident dementia or incident AD.

Conclusion: Low choline intake was associated with increased risk of incident dementia or AD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/ajcn/nqac193DOI Listing
August 2022

Detection of SARS-CoV-2 Specific Antibodies in Saliva Samples.

Front Immunol 2022 8;13:880154. Epub 2022 Jul 8.

Institute of Hepatology, National Clinical Research Center for Infectious Disease, The Third People's Hospital of Shenzhen, The Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China.

Molecular assays on nasopharyngeal swabs act as a confirmatory test in coronavirus disease (COVID-19) diagnosis. However, the technical requirements of nasopharyngeal sampling and molecular assays limit the testing capabilities. Recent studies suggest the use of saliva for the COVID-19 diagnostic test. In this study, 44 patients diagnosed with COVID-19 in The Third People's Hospital of Shenzhen were enrolled. Saliva and serum specimens were obtained at different time points and the immunoglobulins against SARS-CoV-2 were measured. The results showed that saliva IgA presented a higher COI value than IgG and IgM. In matched saliva and serum samples, all saliva samples presented lower IgG levels than serum samples, and only one saliva sample presented a higher IgM level. The conversion rates of saliva IgA and the detection of viral nucleic acids were analyzed in the first and second weeks after hospitalization. The positive rates increased when combining saliva IgA and viral nucleic acid detection. In conclusion, our results provide evidence that saliva IgA could serve as a useful index for the early diagnosis of COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.880154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309249PMC
July 2022

A Prospective Study of Stereotactic Body Radiotherapy (SBRT) with Concomitant Whole-Pelvic Radiotherapy (WPRT) for High-Risk Localized Prostate Cancer Patients Using 1.5 Tesla Magnetic Resonance Guidance: The Preliminary Clinical Outcome.

Cancers (Basel) 2022 Jul 18;14(14). Epub 2022 Jul 18.

Research Department, Hong Kong Sanatorium & Hospital, Happy Valley, Hong Kong SAR, China.

Conventionally fractionated whole-pelvic nodal radiotherapy (WPRT) improves clinical outcome compared to prostate-only RT in high-risk prostate cancer (HR-PC). MR-guided stereotactic body radiotherapy (MRgSBRT) with concomitant WPRT represents a novel radiotherapy (RT) paradigm for HR-PC, potentially improving online image guidance and clinical outcomes. This study aims to report the preliminary clinical experiences and treatment outcome of 1.5 Tesla adaptive MRgSBRT with concomitant WPRT in HR-PC patients. Forty-two consecutive HR-PC patients (72.5 ± 6.8 years) were prospectively enrolled, treated by online adaptive MRgSBRT (8 Gy(prostate)/5 Gy(WPRT) × 5 fractions) combined with androgen deprivation therapy (ADT) and followed up (median: 251 days, range: 20-609 days). Clinical outcomes were measured by gastrointestinal (GI) and genitourinary (GU) toxicities according to the Common Terminology Criteria for Adverse Events (CTCAE) Scale v. 5.0, patient-reported quality of life (QoL) with EPIC (Expanded Prostate Cancer Index Composite) questionnaire, and prostate-specific antigen (PSA) responses. All MRgSBRT fractions achieved planning objectives and dose specifications of the targets and organs at risk, and they were successfully delivered. The maximum cumulative acute GI/GU grade 1 and 2 toxicity rates were 19.0%/81.0% and 2.4%/7.1%, respectively. The subacute (>30 days) GI/GU grade 1 and 2 toxicity rates were 21.4%/64.3% and 2.4%/2.4%, respectively. No grade 3 toxicities were reported. QoL showed insignificant changes in urinary, bowel, sexual, and hormonal domain scores during the follow-up period. All patients had early post-MRgSBRT biochemical responses, while biochemical recurrence (PSA nadir + 2 ng/mL) occurred in one patient at month 18. To our knowledge, this is the first prospective study that showed the clinical outcomes of MRgSBRT with concomitant WPRT in HR-PC patients. The early results suggested favorable treatment-related toxicities and encouraging patient-reported QoLs, but long-term follow-up is needed to confirm our early results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/cancers14143484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321843PMC
July 2022

Effects of dicyandiamide, phosphogypsum and superphosphate on greenhouse gas emissions during pig manure composting.

Sci Total Environ 2022 Jul 20;846:157487. Epub 2022 Jul 20.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Science, China Agricultural University, Beijing 100193, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China. Electronic address:

This study investigated the effects of dicyandiamide, phosphogypsum and superphosphate on greenhouse gas emissions and compost maturity during pig manure composting. The results indicated that the addition of dicyandiamide and phosphorus additives had no negative effect on organic matter degradation, and could improve the compost maturity. Adding dicyandiamide alone reduced the emissions of ammonia (NH), methane (CH) and nitrous oxide (NO) by 9.37 %, 9.60 % and 31.79 %, respectively, which was attributed that dicyandiamide effectively inhibited nitrification to reduce the formation of NO. Dicyandiamide combined with phosphogypsum or superphosphate could enhance mitigation of the total greenhouse gas (29.55 %-37.46 %) and NH emission (18.28 %-21.48 %), which was mainly due to lower pH value and phosphoric acid composition. The combination of dicyandiamide and phosphogypsum exhibited the most pronounced emission reduction effect, simultaneously decreasing the NH, CH and NO emissions by 18.28 %, 38.58 % and 36.14 %, respectively. The temperature and C/N content of the compost were significantly positively correlated with greenhouse gas emissions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2022.157487DOI Listing
July 2022

Ultrahigh efficient emulsification with drag-reducing liquid gating interfacial behavior.

Proc Natl Acad Sci U S A 2022 Jul 11;119(29):e2206462119. Epub 2022 Jul 11.

State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Emulsification is a crucial technique for mixing immiscible liquids into droplets in numerous areas ranging from food to medicine to chemical synthesis. Commercial emulsification methods are promising for high production, but suffer from high energy input. Here, we report a very simple and scalable emulsification method that employs the drag-reducing liquid gating structure to create a smooth liquid-liquid interface for the reduction of resistance and tunable generation of droplets with good uniformity. Theoretical modeling and experimental results demonstrate that our method exhibits ultrahigh efficiency, which can reach up to more than 4 orders of magnitude greater energy-saving compared to commercial methods. For temperature-sensitive biological components, such as enzymes, proteins, and bacteria, it can offer a comfortable environment to avoid exposure to high temperatures during emulsifying, and the interface also enables the suppression of fouling. This unique drag-reducing liquid gating interfacial emulsification mechanism promotes the efficiency of droplet generation and provides fresh insight into the innovation of emulsifications that can be applied in many fields, including the food industry, the daily chemical industry, biomedicine, material fabrication, the petrochemical industry, and beyond.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.2206462119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304007PMC
July 2022

Diagnostic Value and Clinical Application of mNGS for Post-Liver Transplantation Infection: A Cross-Sectional Study With Case Reports.

Front Microbiol 2022 1;13:919363. Epub 2022 Jul 1.

Department for Infectious Diseases, Shenzhen Third People's Hospital, Second Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China.

Liver transplantation is widely acknowledged as the only effective treatment for end-stage liver disease, and infection is reportedly an important cause of postoperative death. Clinical use of metagenomic next-generation sequencing (mNGS) to diagnose postoperative infection and successfully guide drug therapy remains rare. This study included patients with infectious complications after liver transplantation from July 2019 to December 2020 and was divided into three groups: pneumonia, unknown fever, and others (including hepatic failure, kidney failure, cirrhosis after LT, and other postoperative complications that predispose to infection). The mNGS sequencing was used to detect microorganisms, and the results were compared with traditional culture. We found that mNGS yielded improved sensitivity over culture (85.19 vs. 22.22%; <0.0001) but lower specificity (35.71 vs. 89.28%; <0.0001). Among the 48 kinds of pathogens detected, the Torque teno virus 22 (15/122) was the most common, followed by Primate erythroparvovirus 1 (13/122). The top four bacteria included ( = 8), ( = 5), ( = 4), and ( = 4). was the most common fungus. The bronchoalveolar lavage fluid (BALF) exhibited the highest proportion of positive findings among sample types, with viral, fungal, and bacterial mixed infection being the most common ( = 6, 19.35%). Besides, using mNGS for early diagnosis of infection after liver transplantation may effectively prolong patient survival. This is the first study to explore the application value of mNGS and its comparison with traditional culture in pneumonia and other infections in post-liver transplantation patients. The simultaneous application of these two methods suggested that the Torque teno virus 22, , and the are the most common pathogens of viruses, bacteria, and fungi after LT, suggesting that these pathogens may be associated with postoperative pathogen infection and patient prognosis. The mNGS technique showed distinct advantages in detecting mixed, viral, and parasitic infections in this patient population. Further studies are warranted to systematically elucidate the dynamic evolution and molecular characteristics of infection after liver transplantation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2022.919363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283086PMC
July 2022

Development and Clinical Application of a Rapid and Visual Loop-Mediated Isothermal Amplification Test for tetM gene in Clostridioides difficile Strains Cultured from Feces.

Int J Infect Dis 2022 Jul 14. Epub 2022 Jul 14.

Department of Gastroenterology, Integrative Microecology Center, Shenzhen Hospital, Southern Medical University, 1333 New Lake Road, Shenzhen, 518100, China. Electronic address:

Objectives: To develop a rapid and visual loop-mediated isothermal amplification (LAMP) assay targeting the tetM gene in Clostridioides difficile (C. difficile) strains cultured from feces.

Methods: Primers were designed to recognize the tetM gene in C. difficile by LAMP, using turbidity and visual detection. The sensitivity and specificity of LAMP primers was determined. Besides, We conducted both LAMP and polymerase chain reaction (PCR) for the tcdA, tcdB, cdtA, cdtB, ermB, tetM genes in 300 toxigenic C. difficile strains cultured from feces.

Results: The target DNA was amplified and visualized within 60 minutes at a temperature of 62°C. A total of 26 bacterial strains were found negative for tetM, which manifested high specificity of the primers. The detection limit of LAMP was 36.1 pg/µl, which was 100-fold more sensitive than PCR. The positive rate of tetM in toxigenic C. difficile strains cultured from feces was 93.3% by both LAMP and PCR. The proportion of toxin types in those C. difficile strains was 95.7% for A+B+CDT-, 4% for A-B+CDT-, and 0.3% for A+B+CDT+, respectively.

Conclusions: This is the first study examining tetM gene in C. difficile strains cultured from feces by LAMP. Its high specificity and sensitivity, as well as visual detection, make the new assay a powerful diagnostic tool for rapid testing.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijid.2022.07.032DOI Listing
July 2022

SGLT2 inhibitor, canagliflozin, ameliorates cardiac inflammation in experimental autoimmune myocarditis.

Int Immunopharmacol 2022 Jul 13;110:109024. Epub 2022 Jul 13.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Myocarditis is an inflammatory cardiovascular disease which contributes to dilated cardiomyopathy (DCM) and heart failure. Canagliflozin (CANA) exerts anti-inflammatory and cardioprotective effects in heart failure besides its hypoglycemic effect. However, the role of CANA in myocarditis has not been elucidated. In this work, CANA treatment markedly alleviated cardiac inflammation and improved cardiac function in experimental autoimmune myocarditis (EAM) mice induced by α-myosin-heavy chain peptides. The expressions of NLRP3 inflammasome complexes (NLRP3, ASC, and Caspase-1) and their downstream molecules (IL-1β, IL-18) were significantly downregulated by CANA, accompanied with reduced Th17 cell infiltration in hearts. Furthermore, Bax/Bcl-2 ratio, Cleaved Caspase-3 protein level and the percentage of TUNEL-positive myocardial cells, which usually indicated apoptosis, were reduced by CANA treatment. These findings suggest CANA could be a valuable medication for myocarditis treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2022.109024DOI Listing
July 2022

Differential gene screening and bioinformatics analysis of epidermal stem cells and dermal fibroblasts during skin aging.

Sci Rep 2022 Jul 14;12(1):12019. Epub 2022 Jul 14.

Key Laboratory of Dermatology in Integrated Traditional Chinese and Western Medicine, The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, 350003, China.

To explore the differentially expressed genes (DEGs) and potential therapeutic targets of skin aging in GEO database by bioinformatics methods. Dermal fibroblasts and skin aging related data sets GSE110978 and GSE117763 were downloaded from GEO database, and epidermal stem cells and skin aging related data sets GSE137176 were downloaded. GEO2R was used to screen DEGs of candidate samples from the three microarrays, GO function analysis and KEGG pathway analysis were performed. Protein interaction network was constructed using String database, and hub gene was obtained by Cytoscape. NetworkAnalys was used to analyze the coregulatory network of DEGs and MicroRNA (miRNA), interaction with TF, and protein-chemical interactions of DEGs. Finally, DSigDB was used to determine candidate drugs for DEGs. Six DEGs were obtained. It mainly involves the cytological processes such as response to metal ion, and is enriched in mineral absorption and other signal pathways. Ten genes were screened by PPI analysis. Gene-miRNA coregulatory network found that Peg3 and mmu-miR-1931 in DEGs were related to each other, and Cybrd1 was related to mmu-miR-290a-5p and mmu-miR-3082-5p. TF-gene interactions found that the transcription factor UBTF co-regulated two genes, Arhgap24 and Mpzl1. Protein-chemical Interactions analysis and identification of candidate drugs show results for candidate drugs. Try to explore the mechanism of hub gene action in skin aging progression, and to discover the key signaling pathways leading to skin aging, which may be a high risk of skin aging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-16314-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283434PMC
July 2022

MicroRNA-21-5p promotes mucosal type 2 inflammation via regulating GLP1R/IL-33 signaling in chronic rhinosinusitis with nasal polyps.

J Allergy Clin Immunol 2022 Jul 11. Epub 2022 Jul 11.

Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China; Beijing Laboratory of Allergic Diseases and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing 100005, China; Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China. Electronic address:

Background: It has been known that chronic rhinosinusitis with nasal polyps (CRSwNP) is a type 2 inflammation dominated disease, however, the reasons causing such type of mucosal inflammation in CRSwNP are not well elucidated.

Objective: We sought to investigate the role of microRNA-21-5p (miR-21-5p) in regulating mucosal type 2 inflammation in CRSwNP.

Methods: MiR-21-5p expression was detected in nasal mucosa of patients with CRSwNP. Correlations between miR-21-5p and indicators of type 2 inflammation were further analyzed. MiR-21 knockout (KO) mice were used to explore the role of miR-21-5p in a murine model of eosinophilic (E) CRSwNP. Target gene of miR-21-5p related to type 2 inflammation in CRSwNP was identified.

Results: The up-regulated miR-21-5p in nasal mucosa of CRSwNP patients, compared to control subjects, was expressed higher in ECRSwNP than nonECRSwNP patients. MiR-21-5p expression was positively correlated with mucosal eosinophil infiltrations, and the expression of type 2 inflammatory cytokines. In the CRSwNP mice, miR-21KO significantly attenuated type 2 inflammation, as indicated by eosinophil infiltrations and cytokines/chemokines expression in nasal mucosa and lavage fluid; moreover, genes associated with type 2 inflammation were extensively down-regulated at the transcriptome level in miR-21KO mice. Glucagon-like peptide-1 receptor (GLP1R), which was negatively correlated with miR-21-5p expression in human nasal mucosa, was identified as the target of miR-21-5p. Overexpression of miR-21-5p induced IL-33 expression, whereas GLP1R agonist decreased IL-33 production in airway epithelial cells.

Conclusion: MiR-21-5p aggravates type 2 inflammation in nasal mucosa of CRSwNP via targeting GLP1R/IL-33 signaling, which may be a potential therapeutic target for CRSwNP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jaci.2022.05.030DOI Listing
July 2022

Efficacy and Safety of Anlotinib in the Treatment of Small Cell Lung Cancer: A Real-World Observation Study.

Front Oncol 2022 20;12:917089. Epub 2022 Jun 20.

Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Aims: This study aimed to observe the efficacy and safety of anlotinib in the treatment of small cell lung cancer (SCLC) in the real world, as first-line maintenance therapy, second-line, and above.

Methods: Clinical data of 109 patients with SCLC treated with anlotinib and hospitalized at The First Affiliated Hospital of Zhengzhou University from June 2018 to June 2020 were retrospectively analyzed. Analysis of short-term efficacy and survival was performed, with <0.05 being considered statistically significant.

Results: The median progression-free survival (mPFS) of anlotinib monotherapy used as first-line maintenance treatment of SCLC was 6.3 months (11.7 months in the limited phase and 5.8 months in the extensive phase) and median overall survival (mOS) was 16.7 months (not reached in limited phase, 12.6 months in extensive phase). In second-line treatment, anlotinib with chemotherapy prolonged PFS and OS as compared to anlotinib monotherapy (<0.05). In third-line and above treatment, there was no improvement in mPFS with the chemotherapy combination regimen compared to anlotinib monotherapy (3.6 months vs. 3.8 months, .398), with a trend toward impaired mOS (8.5 months vs. not achieved, .060). Univariate analyses and multivariate analyses revealed that Eastern Cooperative Oncology Group performance status and liver metastases were independent prognostic factors affecting PFS and OS. No new anlotinib-related adverse reactions were identified.

Conclusion: Anlotinib was effective for first-line maintenance and second-line treatment, and the chemotherapy combination regimen was superior to monotherapy when applied as second-line treatment. However, this trend was not observed in third-line and above therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.917089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251318PMC
June 2022

A case report of retroperitoneal ectopic pregnancy with lymphatic migration after in vitro fertilization-embryo transfer: an explanation to embryo distant migration.

Fertil Steril 2022 Jul 2. Epub 2022 Jul 2.

Department of Obstetrics and Gynecology, the Third Xiangya Hospital of Central South University, Changsha, Hunan, People's Republic of China. Electronic address:

Objective: To present a rare case of retroperitoneal ectopic pregnancy with lymphatic migration after in vitro fertilization-embryo transfer (IVF-ET) and propose the possible explanation for embryonic migration.

Design: Illustrative video presentation. Images, videos (educational videos), and title slides were used to introduce the case of a patient with retroperitoneal ectopic pregnancy with lymphatic migration after IVF-ET and provide a potential explanation for embryo distant migration in the patient. This work was approved by the institutional review board.

Setting: University hospital.

Patient(s): A 32-year-old woman (gravida 2, para 0) with a history of right salpingectomy was admitted to the hospital 40 days after IVF-ET because of ectopic pregnancy for 1 day; the patient had secondary infertility for 6 years. Gynecologic examination indicated anterior 40-day uterus with no tenderness. A preoperative B-mode ultrasound scan showed that the endometrium was 23 mm, and there was no acoustic image of the pregnant sac in the uterine cavity. Magnetic resonance imaging showed that 1 oval signal measuring approximately 30 × 28 × 35 mm was detected at the gap between the aorta anterior to the third lumbar vertebra and inferior vena cava. The inferior vena cava could be seen on the rear right of the gestational sac, and the abdominal aorta could be seen on the rear left.

Intervention(s): Retroperitoneal ectopic pregnancy with lymphatic migration after IVF-ET was diagnosed using B-mode ultrasound, MRI, and pathology analysis and was removed laparoscopically.

Main Outcome Measure(s): Mechanism analysis of distant ectopic migration in a patient receiving IVF-ET with a history of tubal surgery.

Result(s): The patient was diagnosed using B-mode ultrasound and MRI and underwent laparoscopic surgery to diagnose and remove the retroperitoneal ectopic pregnancy tissue. Further, the pregnancy lesion was completely removed using an ultrasonic knife. The pathological examination showed that the pregnancy tissue was located inside an enlarged lymph node surrounded by lymphoid tissue, and lymphocyte infiltration was also seen in the endometrial tissue, suggesting that the implanted embryo migrated to the retroperitoneum through the lymphatic channels. The patient successfully conceived through IVF-ET 1 year after the operation, and a full-term neonate was delivered by cesarean section.

Conclusion(s): This case reinforces that in patients with a history of tubal surgery, whether unilateral or bilateral, clinicians should pay more attention to the possibility of retroperitoneal pregnancy after IVF-ET and to the follow-up of such patients. The pathological examination report provided evidence that lymphatic migration may be the possible mechanism of retroperitoneal ectopic pregnancy or embryonic migration after intrauterine placement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fertnstert.2022.05.034DOI Listing
July 2022

Efficacy and safety of DBPR108 (prusogliptin) as an add-on to metformin therapy in patients with type 2 diabetes: A 24-week, multi-centre, randomized, double-blind, placebo-controlled, superiority, phase III clinical trial.

Diabetes Obes Metab 2022 Jul 6. Epub 2022 Jul 6.

Key Laboratory of Endocrinology, Ministry of Health, Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Aim: To evaluate the efficacy and safety of DBPR108 (prusogliptin), a novel dipeptidyl peptidase-4 (DPP-4) inhibitor, as an add-on therapy in patients with type 2 diabetes (T2D) that is inadequately controlled with metformin.

Materials And Methods: In this 24-week, multi-centre, randomized, double-blind, placebo-controlled, superiority, phase III study, adult T2D patients with HbA1c levels ranging from 7.0% to 9.5% on stable metformin were enrolled and randomized (2:1) into the DBPR108 + metformin and placebo + metformin groups. The primary endpoint was the change from baseline in HbA1c at week 24 of DBPR108 versus placebo as an add-on therapy to metformin.

Results: At week 24, the least-square mean (standard error) change from baseline in HbA1c was significantly greater in the DBPR108 group (-0.70% [0.09%]) than in the placebo group (-0.07% [0.11%]) (P < .001), with a treatment difference of -0.63% (95% confidence interval: -0.87%, -0.39%) on the full analysis set. A higher proportion of patients achieved an HbA1c of 6.5% or less (19.7% vs. 8.5%) and an HbA1c of 7.0% or less (50.0% vs. 21.1%) at week 24 in the DBPR108 + metformin group. Furthermore, add-on DBPR108 produced greater reductions from baseline in fasting plasma glucose and 2-hour postprandial plasma glucose without causing weight gain. The overall frequency of adverse events was similar between the two groups.

Conclusions: DBPR108 as add-on therapy to metformin offered a significant improvement in glycaemic control, was superior to metformin monotherapy (placebo) and was safe and well-tolerated in patients with T2D that is inadequately controlled with metformin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/dom.14810DOI Listing
July 2022

New Insights into Human Biotransformation of BDE-209: Unique Occurrence of Metabolites of Ortho-Substituted Hydroxylated Higher Brominated Diphenyl Ethers in the Serum of e-Waste Dismantlers.

Environ Sci Technol 2022 07 5;56(14):10239-10248. Epub 2022 Jul 5.

State Key Laboratory of Organic Geochemistry, Guangdong Key Laboratory of Environment Protection and Resource Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, Guangdong 510640, China.

Extremely high levels of decabromodiphenyl ether (BDE-209) are frequently found in the serum of occupationally exposed groups, such as e-waste dismantlers and firefighters. However, the metabolism of BDE-209 in the human body is not adequately studied. In this study, 24 serum samples were collected from workers at a typical e-waste recycling workshop in Taizhou, Eastern China, and the occurrence and fate of these higher brominated diphenyl ethers (PBDEs) were investigated. The median concentration of the total PBDEs in the serum was 199 ng/g lipid weight (lw), ranging from 125 to 622 ng/g lw. Higher brominated octa- to deca-BDEs accounted for more than 80% of the total PBDEs. Three ortho-hydroxylated metabolites of PBDEs─6-OH-BDE196, 6-OH-BDE199, and 6'-OH-BDE206─were widely detected with a total concentration (median) of 92.7 ng/g lw. The concentrations of the three OH-PBDEs were significantly higher than their octa- and nona-PBDE homologues, even exceeding those of the total PBDEs in several samples, indicating that the formation of OH-PBDEs was a major metabolic pathway of the higher brominated PBDEs in occupationally exposed workers. An almost linear correlation between 6-OH-BDE196 and 6-OH-BDE199 ( = 0.971, < 0.001) indicates that they might undergo a similar biotransformation pathway in the human body or may be derived from the same precursor. In addition, the occurrence of a series of penta- to hepta- ortho-substituted OH-PBDEs was preliminarily identified according to their unique "predioxin" mass spectral profiles by GC-ECNI-MS. Taken together, the tentative metabolic pathway for BDE-209 in e-waste dismantlers was proposed. The oxidative metabolism of BDE-209 was mainly observed at the ortho positions to form 6'-OH-BDE-206, which later underwent a consecutive loss of bromine atoms at the meta or para positions to generate other -OH-PBDEs. Further studies are urgently needed to identify the chemical structures of these -OH-PBDE metabolites, and perhaps more importantly to clarify the potentially toxic effects, along with their underlying molecular mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.est.2c02074DOI Listing
July 2022

A Multiobjective Hybrid Optimization Algorithm for Path Planning of Coal Mine Patrol Robot.

Comput Intell Neurosci 2022 23;2022:9094572. Epub 2022 Jun 23.

School of Mechanical Engineering, Liaoning Technical University, Fuxin 123000, China.

In the complex underground environment, the paths planned for coal mine patrol robot are often too long and unsmooth under the influence of low visibility and poor road conditions. To solve the problems, this paper improves the hybrid algorithm between the improved artificial fish swarm algorithm (AFSA) and the dynamic window algorithm (DWA) for global path planning of coal mine patrol robot and introduces the improved genetic algorithm (GA) to enhance the path planning accuracy. Based on the global optimal path, the improved DWA was adopted to design a new adaptive trajectory evaluation function, which improves the ability of the patrol robot to avoid local obstacles. The proposed optimization algorithm was proved feasible through simulations. In addition, a simulation platform for the control of coal mine patrol robot was established, using the software development platform for coal mine patrol robot and robot operating system (ROS). The simulation results show that the improvement shortened the path length by 0.12 m, reduced the time by 3.14 s, and removed many turning points and redundant points. Therefore, the proposed improved hybrid path planning algorithm is effective and superior.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/9094572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246641PMC
July 2022

Antiviral Efficacy and Safety of Molnupiravir Against Omicron Variant Infection: A Randomized Controlled Clinical Trial.

Front Pharmacol 2022 15;13:939573. Epub 2022 Jun 15.

Shenzhen Key Laboratory of Pathogen and Immunity, National Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China.

The rapid worldwide spread of the Omicron variant of SARS-CoV-2 has unleashed a new wave of COVID-19 outbreaks. The efficacy of molnupiravir, an approved drug, is still unknown in patients infected with the Omicron variant. Evaluated the antiviral efficacy and safety of molnupiravir in patients infected with SARS-CoV-2 Omicron variant, with symptom duration within 5 days. We conducted a randomized, controlled trial involving patients with mild or moderate COVID-19. Patients were randomized to orally receive molnupiravir (800 mg) plus basic treatment or only basic treatment for 5 days (BID). The antiviral efficacy of the drug was evaluated using reverse transcriptase polymerase chain reaction. Results showed that the time of viral RNA clearance (primary endpoint) was significantly decreased in the molnupiravir group (median, 9 days) compared to the control group (median, 10 days) (Log-Rank = 0.0092). Of patients receiving molnupiravir, 18.42% achieved viral RNA clearance on day 5 of treatment, compared to the control group (0%) ( = 0.0092). On day 7, 40.79%, and 6.45% of patients in the molnupiravir and control groups, respectively, achieved viral RNA clearance ( = 0.0004). In addition, molnupiravir has a good safety profile, and no serious adverse events were reported. Molnupiravir significantly accelerated the SARS-CoV-2 Omicron RNA clearance in patients with COVID-19. [chictr.org.cn], identifier [ChiCTR2200056817].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2022.939573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248931PMC
June 2022

Procyanidins from hawthorn () alleviate lipid metabolism disorder inhibiting insulin resistance and oxidative stress, normalizing the gut microbiota structure and intestinal barrier, and further suppressing hepatic inflammation and lipid accumulation.

Food Funct 2022 Jul 18;13(14):7901-7917. Epub 2022 Jul 18.

Department of Nutrition and Food Safety, College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, P. R. China.

Recently, lipid metabolism disorder (LMD) has been regarded as a risk factor leading to multiple diseases and affecting human health. Procyanidins have been reported to be a potential therapy for LMD. This study explored the effect and possible mechanism of procyanidins from hawthorn (HPC) in LMD rats fed a high-fat diet. HPC supplementation for 8 weeks significantly relieved lipid accumulation in the serum and liver, and protected the structure of liver in LMD rats. The amelioration of insulin resistance, lipid metabolism related hormones and oxidative stress was also found. Moreover, HPC also regulated the structure of gut microbiota, especially increased the abundances of , and , and decreased , , , and . Additionally, HPC enriched genes were involved in the fatty acid biosynthesis, MAPK, and lipopolysaccharide biosynthesis pathways. We found a high LPS level due to the microbiome disorder induced by the high-fat diet, and then western blot results showed that HPC maintained the intestinal barrier and mitigated inflammation and lipid accumulation in the liver regulating the NF-κB and AMPK pathways, which were related to LMD. This study provided new insights into dietary intervention based on HPC to alleviate LMD stabilizing the gut-liver axis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2fo00836jDOI Listing
July 2022

Single-cell RNA sequencing data analysis suggests the cell-cell interaction patterns of the pituitary-kidney axis.

Sci Rep 2022 Jul 1;12(1):11147. Epub 2022 Jul 1.

Department of Nephrology, Guizhou Provincial People's Hospital, 83, Zhongshan Road, Nanming District, Guiyang, 550002, Guizhou, China.

Kidney functions, including electrolyte and water reabsorption and secretion, could be influenced by circulating hormones. The pituitary gland produces a variety of hormones and cytokines; however, the influence of these factors on the kidney has not been well explained and explored. To provide more in-depth information and insights to support the pituitary-kidney axis connection, we used mouse pituitary and kidney single-cell transcriptomics data from the GEO database for further analysis. Based on a ligand-receptor pair analysis, cell-cell interaction patterns between the pituitary and kidney cell types were described. Key ligand-receptor pairs, such as GH-GHR, PTN-SDC2, PTN-SDC4, and DLK1-NOTCH3, were relatively active in the pituitary-kidney axis. These ligand-receptor pairs mainly target proximal tubule cells, principal cells, the loop of Henle, intercalated cells, pericytes, mesangial cells, and fibroblasts, and these cells are related to physiological processes, such as substance reabsorption, angiogenesis, and tissue repair. Our results suggested that the pituitary gland might directly regulate kidney function by secreting multiple hormones or cytokines and indicated that the above ligand-receptor pairs might represent a new research focus for studies on kidney function or kidney disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-14680-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249760PMC
July 2022

PPARG Silencing Improves Blood Pressure Control and Alleviates Renal Damage by Modulating RAS Circadian Rhythm in Hypertensive Rats.

Ann Clin Lab Sci 2022 May;52(3):452-461

Center of Cardiology and Hebei General Hospital, Shijiazhuang, Hebei, China.

Objective: Peroxisome proliferator-activated receptor gamma (PPARG) polymorphisms are associated with hypertension, but the role of PPARG in hypertensive nephropathy is poorly understood.

Methods: Male Sprague-Dawley rats were applied to construct renovascular hypertension model by 2-kid-ney, 1-clip (2K1C) method. Tail vein bolus injection of adeno-associated virus (rAAV)-shPPARG was performed to knockout PPARG in 2K1C rats. The heart rate (HR), systolic pressure (SBP), diastolic pressure (DBP) and activity of rats were monitored after treatments. The role of PPARG in hypertension, renal damage, and circadian rhythm of renin-angiotensin system (RAS) was explored by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), western blot, Masson staining, hematoxylin eosin (HE) staining, Sirius red staining and enzyme-linked immunosorbent assay.

Results: PPARG was over-expressed in thoracic aortas of 2K1C rats. 2K1C treatment enhanced DBP and SBP in rats, which was reversed by PPARG silencing. PPARG silencing alleviated 2K1C-induced renal damage. 2K1C treatment reduced angiotensin II and increased angiotensin converting enzyme (ACE) and plasma renin activity (PRA) concentrations in rat plasma during the light period and decreased plasma PRA concentration during the dark period, which were all overturned by PPARG silencing. PPARG silencing effectively improved the RAS circadian rhythm in hypertension.

Conclusion: PPARG silencing improved blood pressure control and alleviated renal damage by regulating RAS circadian rhythm in hypertensive rats.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2022

Inner membrane complex proteomics reveals a palmitoylation regulation critical for intraerythrocytic development of malaria parasite.

Elife 2022 07 1;11. Epub 2022 Jul 1.

State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China.

Malaria is caused by infection of the erythrocytes by the parasites . Inside the erythrocytes, the parasites multiply via schizogony, an unconventional cell division mode. The inner membrane complex (IMC), an organelle located beneath the parasite plasma membrane, serving as the platform for protein anchorage, is essential for schizogony. So far, the complete repertoire of IMC proteins and their localization determinants remain unclear. Here we used biotin ligase (TurboID)-based proximity labeling to compile the proteome of the schizont IMC of the rodent malaria parasite . In total, 300 TurboID-interacting proteins were identified. 18 of 21 selected candidates were confirmed to localize in the IMC, indicating good reliability. In light of the existing palmitome of , 83 proteins of the IMC proteome are potentially palmitoylated. We further identified DHHC2 as the major resident palmitoyl-acyl-transferase of the IMC. Depletion of DHHC2 led to defective schizont segmentation and growth arrest both in vitro and in vivo. DHHC2 was found to palmitoylate two critical IMC proteins CDPK1 and GAP45 for their IMC localization. In summary, this study reports an inventory of new IMC proteins and demonstrates a central role of DHHC2 in governing the IMC localization of proteins during the schizont development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7554/eLife.77447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293000PMC
July 2022

Co-composting of kitchen waste with agriculture and forestry residues and characteristics of compost with different particle size: An industrial scale case study.

Waste Manag 2022 Jul 25;149:313-322. Epub 2022 Jun 25.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Science, China Agricultural University, Beijing 100193, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China. Electronic address:

Since the implementation of domestic waste classification in China, the kitchen waste production has increased rapidly. The unique physical and chemical properties of kitchen waste make it impossible for direct composting for composting alone. This study investigated the co-composting of kitchen waste with agriculture and forest residues at an industrial scale at the Nangong Composting Plant (Located in Beijing). Cornstalks, garden waste, and watermelon seedlings were composted with kitchen waste, with the added agriculture and forestry residues comprising 5%, 10% and 20% of the weight. Industrial composting was performed 30 days at a scale of 165-180 tone. The mixed compost products were screened to different particle sizes, and the maturity, humification, and calorific value were analyzed. The kitchen waste mixed with 20% agricultural complementary materials reached hyperthermophilic temperature (82 °C), had reduced moisture content (45%), and resulted in better composting performance at an industrial scale. By adding 20% complementary materials to kitchen waste produced mature compost with a higher germination index (GI) (91%) by adjusting the pH, electrical conductivity (EC), carbon to nitrogen ratio (C/N), and moisture content. The compost in the 5% and 10% complementary materials treatments did not fully mature and had a GI of<10%, influenced by the higher EC and NH-N content. The property of final compost with different particle size vary greatly. The small particle size compost (≤45 mm) had higher uniformity, maturity, and humification degree, and it was suitable to use as a fertilizer; the larger particle size (>45 mm) had more material with lower calorific value (8000-10,000 kJ·kg), and could be used as refuse-derived fuel. To make better use of kitchen waste compost, 45-mm particle size screening is suggested at an industrial-scale composting plant. These results support industrial-scale kitchen waste composting in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.wasman.2022.06.029DOI Listing
July 2022

Republic of Singapore Air Force Helicopter Search-and-Rescue and Medical Evacuations: A 5-Year Review.

Air Med J 2022 Jul-Aug;41(4):350-358. Epub 2022 May 27.

Republic of Singapore Air Force, Singapore.

The Republic of Singapore Air Force (RSAF) provides Helicopter Search-and-Rescue (SAR) and Helicopter Medical Evacuation (Heli-Medevac) coverage for the Singapore Aeronautical Search and Rescue Region (ASSR) in the South China Sea, spanning 840,000 km. This region contains busy international shipping lanes and air traffic routes. Each year, Singapore's Helicopter SAR and Heli-Medevac service is activated multiple times to rescue personnel lost at sea or to evacuate ill and injured ship sailors or passengers to tertiary hospitals in Singapore for stabilization and advanced care. This is a retrospective review on all civilian SAR and Heli-medevac activations by the RSAF over a 5-year period from 2016 to 2020. Case profiles, presenting conditions, in-flight treatment, and patient outcomes are reviewed and discussed. Key operational observations made from RSAF's SAR and Heli-Medevac, as well as lessons learned from these missions, are discussed in this article.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.amj.2022.04.012DOI Listing
June 2022

Impact of National Volume-Based Procurement on the Procurement Volumes and Spending for Antiviral Medications of Hepatitis B Virus.

Front Pharmacol 2022 6;13:842944. Epub 2022 Jun 6.

Department of Pharmacy Administration & Clinical Pharmacy, School of Pharmaceutical Sciences, Peking University, Beijing, China.

Although persistent inhibition of HBV replication by antiviral therapy has shown to slow disease progression, cost-related access barriers to these essential medicines are becoming salient. The national volume-based procurement (NVBP) was piloted in China and led to substantial reduction in the list price of prescription drugs. To examine the impact of NVBP on selected antiviral medication costs per defined daily dose (DDD), procurement volumes, and spending. We employed an interrupted time series design to examine changes in cost per defined daily dose (DDD), procurement volumes, and spending for NVBP bid-winning antiviral medications (tenofovir disoproxil fumarate and entecavir) in 11 pilot cities from 2017 to 2020. Procurement transaction data were obtained from 9,454 hospitals in the Chinese Hospital Pharmaceutical Audit (CHPA) database. In the secondary analysis, the control group comprised two non-NVBP drugs (adefovir and lamivudine) procured in 11 cities not exposed to the NVBP. Cost per DDD of the two hepatitis B virus (HBV) antiviral medications reduced by CNY1.598 ( = 0.002) immediately following the implementation of NVBP, dropping from an average cost of CNY16.483 per DDD at baseline to CNY6.420 at the end of the observation period. NVBP implementation resulted in a substantial reduction in daily costs of antivirals and an increase in monthly procurement volumes by 6.674 million DDDs ( = 0.017), while monthly spending was reduced by CNY138.26 million ( = 0.002). In the secondary ITS analysis with a control group, the average cost per DDD of the NVBP bid-winning antivirals declined by CNY4.537 ( < 0.001), monthly procurement volumes increased by 7.209 million DDDs ( = 0.002), and monthly spending dropped by CNY138.83 million ( < 0.001). Volume-based procurement piloted in China may be effective for reducing price and total expenditures and improving drug utilization, which is especially important for HBV patients who need constant access to antiviral therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2022.842944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207460PMC
June 2022

Potential selection bias in the baseline.

Resuscitation 2022 Jun 18. Epub 2022 Jun 18.

Department of Anesthesiology, Chongqing University Central Hospital, Chongqing Emergency Medical Center, Chongqing, PR China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.resuscitation.2022.06.009DOI Listing
June 2022

Multimodal deep learning for Alzheimer's disease dementia assessment.

Nat Commun 2022 06 20;13(1):3404. Epub 2022 Jun 20.

Department of Neurology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Worldwide, there are nearly 10 million new cases of dementia annually, of which Alzheimer's disease (AD) is the most common. New measures are needed to improve the diagnosis of individuals with cognitive impairment due to various etiologies. Here, we report a deep learning framework that accomplishes multiple diagnostic steps in successive fashion to identify persons with normal cognition (NC), mild cognitive impairment (MCI), AD, and non-AD dementias (nADD). We demonstrate a range of models capable of accepting flexible combinations of routinely collected clinical information, including demographics, medical history, neuropsychological testing, neuroimaging, and functional assessments. We then show that these frameworks compare favorably with the diagnostic accuracy of practicing neurologists and neuroradiologists. Lastly, we apply interpretability methods in computer vision to show that disease-specific patterns detected by our models track distinct patterns of degenerative changes throughout the brain and correspond closely with the presence of neuropathological lesions on autopsy. Our work demonstrates methodologies for validating computational predictions with established standards of medical diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-31037-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209452PMC
June 2022

Plasma miR-193b-3p Is Elevated in Type 2 Diabetes and Could Impair Glucose Metabolism.

Front Endocrinol (Lausanne) 2022 27;13:814347. Epub 2022 May 27.

Department of Occupational and Environmental Health and State Key Laboratory of Environmental Health for Incubating, School of Public Health, Huazhong University of Science and Technology, Wuhan, China.

Objective: To explore differentially expressed miRNAs in type 2 diabetes and their potential cellular functions.

Methods: We screened plasma miRNAs by miRNA array analysis and validated them by TaqMan real-time PCR in 113 newly diagnosed, untreated type 2 diabetes cases and 113 healthy controls. Low-abundance plasma proteins encoded by miR-193b-3p target genes were explored in this study population. We further investigated the potential cellular functions of the differentially expressed miRNAs in HepG2 cells.

Results: miR-193b-3p was differentially expressed in type 2 diabetes cases compared to healthy controls (fold change = 2.01, = 0.006). Plasma levels of triosephosphate isomerase (TPI1, a protein involved in the glycolytic pathway) decreased in type 2 diabetes cases (fold change = 1.37, = 0.002). The effect of miR-193b-3p on TPI1 was verified by transfection of miR-193b-3p into HepG2 cells. miR-193b-3p inhibited the expression of YWHAZ/14-3-3ζ in the PI3K-AKT pathway, subsequently altering the expression of FOXO1 and PCK1. After transfection, cells were incubated in glucose-free medium for another 4 h. Glucose levels in medium from cells with elevated miR-193b-3p levels were significantly higher than those in medium from negative control cells ( = 0.016). In addition, elevated miR-193b-3p reduced glucose uptake by inhibiting insulin receptor (IR) and GLUT2 expression.

Conclusion: Plasma miR-193b-3p levels increased in type 2 diabetes cases, and TPI1 levels decreased in both plasma and HepG2 cells with increased miR-193b-3p levels, while extracellular lactate levels did not significantly changed. Moreover, miR-193b-3p may affect glucose metabolism by directly targeting YWHAZ/14-3-3ζ and upregulating the transcription factor FOXO1 downstream of the PI3K-AKT pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2022.814347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197112PMC
May 2022

Comparison of Bone Mineral Density in US Adults With Diabetes, Prediabetes and Normoglycemia From 2005 to 2018.

Front Endocrinol (Lausanne) 2022 30;13:890053. Epub 2022 May 30.

Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Background: Accumulating evidence has shown that diabetes has an impact on bone metabolism with conflicting results. Furthermore, little is known about the relationship of prediabetes with bone mineral density (BMD). Therefore, we explored the association between BMD and glucometabolic status in adults in the US.

Methods: In this cross-sectional study, we extracted and analyzed data from the National Health and Nutrition Examination Survey (NHANES) from 2005 to 2018. A total of 14610 subjects aged 40 ≥ years diagnosed with diabetes, prediabetes, or normal glucose regulation (NGR) and had available data on BMD were eligible.

Results: The prevalence of prediabetes and diabetes in US adults aged 40 ≥ years were 39.2% and 26.4%, respectively. After multivariable adjustment, we found an increasing trend of BMD at the total hip, femoral neck, and lumbar spine with glucometabolic conditions from NGR and prediabetes to diabetes in adults aged ≥ 40 years in the US. This trend was more prominent in women than that in men. Fasting plasma glucose (FPG) and HbA1c levels were also positively correlated with BMD.

Conclusions: Glucometabolic conditions were significantly associated with BMD values in US adults.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2022.890053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195625PMC
May 2022
-->