Publications by authors named "Jing Yu"

1,722 Publications

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Older Adults' Subjective Cognitive Decline Correlated with Subjective but Not Objective Sleep: A Mediator Role of Depression.

Int J Aging Hum Dev 2021 Jun 14:914150211024186. Epub 2021 Jun 14.

26463 Faculty of Psychology, Southwest University, Chongqing, China.

This study examined the relationship between older adults' subjective versus objective sleep and subjective cognitive decline (SCD), and explored the role of depression on this association. One hundred and four community-dwelling older adults underwent a week of actigraphic sleep monitoring, and completed a series of neuropsychological screeners. Older adults'SCD score was positively correlated with subjective insomnia, but not with objective sleep parameters. Further mediation modeling revealed that older adults'depression mediated the association between subjective insomnia and SCD. Subjective sleep, rather than objective sleep, may be a more sensitive indicator for older adults' SCD, with depression symptoms appearing to account for most of the variance. These findings extend our perspective on the relationship between sleep disruption and cognitive decline, and highlight the necessity of early targeted interventions on sleep to reduce the risk of cognitive impairment in the elderly with SCD.
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http://dx.doi.org/10.1177/00914150211024186DOI Listing
June 2021

Association of circulating fibroblast growth factor-2 with progression of HIV-chronic kidney diseases in children.

Pediatr Nephrol 2021 Jun 14. Epub 2021 Jun 14.

Child Health Research Center, Department of Pediatrics, School of Medicine, University of Virginia, Room 2120, MR4 Building, 409 Lane Road, Charlottesville, VA, 22908, USA.

Background: Children living with HIV frequently show high plasma levels of fibroblast growth factor-2 (FGF-2/bFGF). FGF-2 accelerates the progression of several experimental kidney diseases; however, the role of circulating FGF-2 in childhood HIV-chronic kidney diseases (HIV-CKDs) is unknown. We carried out this study to determine whether high plasma FGF-2 levels were associated with the development of HIV-CKDs in children.

Methods: The plasma and urine FGF-2 levels were measured in 84 children (< 12 years of age) living with HIV during the pre-modern antiretroviral era, and followed for at least 3 years to determine the prevalence of proteinuria and HIV-CKDs. We also assessed the distribution of the kidney FGF-2 binding sites by autoradiography and Alcian blue staining, and explored potential mechanisms by which circulating FGF-2 may precipitate HIV-CKDs in cultured kidney epithelial and mononuclear cells derived from children with HIV-CKDs.

Results: High plasma FGF-2 levels were associated with a high viral load. Thirteen children (~ 15%) developed HIV-CKDs and showed a large reservoir of FGF-2 low-affinity binding sites in the kidney, which can facilitate the recruitment of circulating FGF-2. Children with high plasma and urine FGF-2 levels had 73-fold increased odds (95% CI 9-791) of having HIV-CKDs relative to those with normal FGF-2 values. FGF-2 induced the proliferation and decreased the expression of APOL-1 mRNA in podocytes, and increased the attachment and survival of infected mononuclear cells cultured from children with HIV-CKDs.

Conclusions: High plasma FGF-2 levels appear to be an additional risk factor for developing progressive childhood HIV-CKDs.
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http://dx.doi.org/10.1007/s00467-021-05075-yDOI Listing
June 2021

Fucoidan Extracted From Sporophyll of Grown in Weihai, China - Chemical Composition and Comparison of Antioxidant Activity of Different Molecular Weight Fractions.

Front Nutr 2021 28;8:636930. Epub 2021 May 28.

Faculty of Health and Environmental Sciences, School of Public Health and Interdisciplinary Studies, Auckland University of Technology, Auckland, New Zealand.

Fucoidan is a multifunctional marine carbohydrate polymer that differs in its chemical composition and bioactivity both between seaweed species and within species from different locations across the globe. In this study, fucoidan was extracted from the sporophyll of grown in Weihai, Shandong Province, China. Fucoidan fractions with molecular weight cutoffs (MWCO) of >300 kDa and <10 kDa were obtained via dialysis. The fucoidan standard from Sigma (Fstd, ≥95, CAS: 9072-19-9), fucoidan crude extract (WH), >300 kDa fraction (300k) and <10 kDa fraction (10k) were compared in terms of chemical composition and antioxidant capacity. Based on Fourier transform infrared spectroscopy (FT-IR) analysis, Fstd, WH, and 300k all showed strong bands around 830 cm, corresponding to the sulfate substituent in the molecule. The results showed that compared with WH and 300 k, the degree of sulfation at 10k was the lowest. From Nuclear magnetic resonance spectroscopy (NMR) result, the four fucoidan samples all contain α--fucose. The primary antioxidant ability of the 10k is significantly higher than that of the 300k, WH, and Fstd, but the secondary antioxidant capabilities of the 10k and 300k were similar, and both were higher than that of the butylated hydroxyanisole (BHA). The ferric reducing antioxidant ability was higher in the 300k and WH fractions. This demonstrates that fucoidan extracted from grown in Weihai, China should be a useful nutraceutical resource.
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http://dx.doi.org/10.3389/fnut.2021.636930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193228PMC
May 2021

Rare DNA Mismatch Repair-Related Protein Loss in Patients with Intrahepatic Cholangiocarcinoma and Combined Hepatocellular-Cholangiocarcinoma and Their Response to Immunotherapy.

Cancer Manag Res 2021 31;13:4283-4290. Epub 2021 May 31.

Department of Abdominal Cancer, West China Medical School, Cancer Center, West China Hospital, Sichuan University, Chengdu, People's Republic of China.

Purpose: The patients with advanced mismatch repair deficiency (dMMR) cancers can benefit from programmed cell death 1 (PD-1) pathway blockade, regardless of the tumor type. Little is known about the prevalence of dMMR in intrahepatic cholangiocarcinoma (ICC) and combined hepatocellular-cholangiocarcinoma (cHCC-CC). This study aimed to assess the mismatch repair (MMR)-related protein expression in patients with ICC and cHCC-CC.

Patients And Methods: Formalin-fixed, paraffin-embedded tumor specimens were obtained from patients undergoing surgery at the West china Hospital between 2009 and 2017. The immunoreactions for MLH1, MSH2, MSH6, and PMS2 were investigated to determine the MMR status.

Results: A total of 97 patients were evaluated, including 73 ICC patients and 24 cHCC-CC patients. The prevalence of dMMR was only found in two cases of 97 patients (2.06%). Both patients are ICC. In 24 cHCC-CC patients, no dMMR was observed. They did not receive an adjuvant chemotherapy after surgery. At the end of the follow-up, one patient was in a tumor-free state, and the other patient had local recurrence and metastasis. After receiving sintilimumab (an immune checkpoint inhibitor [ICI] for PD- 1), the patient had a partial response.

Conclusion: DMMR was detected in few patients with ICC and cHCC-CC. Thus, it is not recommended to routinely evaluate the MMR status of patients with ICC or cHCC-CC after surgery, but that of patients with advanced ICC or cHCC-CC should be assessed.
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http://dx.doi.org/10.2147/CMAR.S304281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183674PMC
May 2021

Serotonin 2A receptor function and depression-like behavior in rats model of hypothyroidism.

Exp Brain Res 2021 Jun 9. Epub 2021 Jun 9.

Dalian Medical University, Dalian, 116044, China.

Hypothyroidism causes somatic, psychosocial and affective psychosis, including depression-like behaviors. In this study, (hypothyroidism group; HP group) adult male Sprague Dawley (SD) rats were induced to hypothyroidism after 5 weeks of exposure to 0.05% propylthiouracil (PTU) in potable water, control animals (CON group) were given the same amount of water. The following behavioral experiments were conducted, respectively: open-field test (OFT), forced swimming test (FST), tail suspension test (TST). TT[Formula: see text] and TT[Formula: see text] levels were measured after the behavior tests and the expression levels of 5-HT[Formula: see text] receptor and 5-HT[Formula: see text] receptor proteins were analyzed in the hippocampus and prefrontal cortex. The level of TT[Formula: see text] and TT[Formula: see text] in the HP group rats was much lower than that in the CON group. The hypothyroid rats also showed weight loss, much longer immobility time in tail suspension test and forced swimming test. Besides, 5 weeks of PTU administration was associated with significantly decreased expression levels of 5-HT[Formula: see text] receptor and 5-HT[Formula: see text] receptor proteins compared with control group, which were significantly negatively correlated with immobility time in FST and TST. In conclusion, our results suggest that hypothyroidism induces depressive behaviors through the influence of the serotonin system, and the decreased expression of the 5-HT[Formula: see text] receptor is an important cause of the depressive behaviors in hypothyroidism.
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http://dx.doi.org/10.1007/s00221-021-06129-1DOI Listing
June 2021

Association of free androgen index and sex hormone-binding globulin and left ventricular hypertrophy in postmenopausal hypertensive women.

J Clin Hypertens (Greenwich) 2021 Jun 9. Epub 2021 Jun 9.

Lanzhou University Second College of Clinical Medicine, Lanzhou, China.

The aim of the present study was to explore the relationship between androgen and LVH in postmenopausal hypertensive women. Enrolled in this study were 378 postmenopausal hypertensive women who were admitted to the department of cardiology between December 2018 and December 2020. According to left ventricular mass index (LVMI) evaluated by echocardiography, the patients were divided into LVH group (n = 172) and non-LVH group (n = 206). Their clinical characteristics were collected. Based on the result of propensity score matching analysis, 160 cases in each group were matched successfully. After correcting for confounding factors by various models, the results showed that free androgen index (FAI) and sex hormone-binding globulin (SHBG) were the influencing factors of LVH in postmenopausal women with hypertension. Patients with elevated SHBG were 5% less likely to develop LVH than those without elevated SHBG (OR: 0.950, 95% CI 0.922-1.578). Postmenopausal hypertensive patients with elevated FAI were 16% more likely to have LVH than those without elevated FAI (OR: 1.608, 95% CI 0.807-3.202). Multiple linear regression showed that LVMI increased by 61.82g/m for every 1 unit increase in FAI. In addition, SHBG decreased by 1 nmol/l, and LVMI increased by 0.177g/m . Subgroup analysis showed that patients in the controlled BP group had a lower risk of LVH for every additional unit of SHBG compared with the uncontrolled BP group. The risk of LVH for each additional unit of FAI in the uncontrolled BP group was higher than that in the controlled BP group. The results of this present study showed that the occurrence of LVH was positively correlated with FAI and negatively correlated with SHBG in postmenopausal women with hypertension. The increase in FAI level and the decrease in SHBG level may be related to the occurrence and development of LVH in postmenopausal hypertension.
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http://dx.doi.org/10.1111/jch.14301DOI Listing
June 2021

Circulating Fibroblast Growth Factor-2 precipitates HIV-nephropathy in mice.

Dis Model Mech 2021 Jun 9. Epub 2021 Jun 9.

Child Health Research Center, Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, VA, USA.

Over 80% of all children living with HIV reside in Africa and are at risk of developing HIV-associated nephropathy (HIVAN). Once HIVAN is established in children, it is difficult to revert its progression to chronic kidney failure even using antiretroviral drugs. Therefore, new therapeutic strategies are needed. Previous studies showed that the risk of developing HIVAN increases in children with high circulating levels of FGF-2, but it is unclear whether FGF-2 per se precipitates HIVAN. To unravel the role of circulating FGF-2 in childhood HIVAN, we used the HIV-Tg26 mouse model of HIVAN. Briefly, we demonstrated that circulating FGF-2 was preferentially recruited in the kidney of HIV-Tg26 mice with renal disease, and precipitated HIVAN in young mice without pre-existing kidney disease by activating the pERK pathway in renal epithelial cells without previously inducing the expression of HIV-1 genes. Wild type mice injected with recombinant adenoviral FGF-2 vectors (rAd-FGF-2) carrying a secreted form of human FGF-2 developed transient and reversible HIVAN-like lesions, including proteinuria and glomerular enlargement. HIV-Tg26 mice injected with rAd-FGF-2 developed more significant proliferative and pro-fibrotic inflammatory lesions, similar to those seen in childhood HIVAN. These lesions were partially reversed in mice treated with the FGF/VEGF receptor tyrosine kinase inhibitor PD173074. In conclusion, we developed a new FGF-2-inducible model of childhood HIVAN, and showed that high circulating levels of FGF-2 precipitated HIVAN without inducing the renal expression of HIV-genes. These findings suggest that high plasma FGF-2 levels may be an independent risk factor for precipitating HIVAN in young children.
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http://dx.doi.org/10.1242/dmm.048980DOI Listing
June 2021

Selective Inhibition of 11β-Hydroxysteroid Dehydrogenase Type 1 Attenuates High-Fat Diet-Induced Hepatic Steatosis in Mice.

Drug Des Devel Ther 2021 31;15:2309-2324. Epub 2021 May 31.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genomic Resource Center, Medical College of Soochow University, Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China.

Introduction: The effect of 11β-hydroxysteroid dehydrogenase type1 (11β-HSD1) inhibition on hepatic steatosis is incompletely understood. Here, we aimed to determine the therapeutic effect of BVT.2733, a selective 11β-HSD1 inhibitor, on hepatic steatosis.

Materials And Methods: C57B/6J mice were randomly divided into a low-fat diet (LFD) fed group and a high-fat diet (HFD) fed group. Mice were fed with HFD for 28 weeks which induced obesity and severe hepatic steatosis. The two groups were further divided into four groups as follows: LFD, LFD with BVT.2733, HFD, and HFD with BVT.2733. Mice in LFD+BVT and HFD+BVT groups were intraperitoneally injected with BVT.2733 daily for 30 days. Effects of BVT.2733 on mice body weight, serum lipid profile, serum free fatty acids (FFAs), glucocorticoid levels, gene expression in adipose and liver tissues were assessed.

Results: Injection of a low dose of BVT.2733 (50 mg/kg/day) reduced body weight and hyperlipidemia, but did not improve glucose tolerance and insulin resistance in diet-induced obese mice. The low dose of BVT.2733 attenuated hepatic steatosis, liver injury, and liver lipolytic gene expression in diet-induced obese mice. Besides, the low dose of BVT.2733 reduced fat mass and lipolysis in visceral adipose tissues, hepatic FFAs, and serum corticosterone levels in diet-induced obese mice.

Conclusion: Our study shows that moderate inhibition of 11β-HSD1 by BVT.2733 reduces FFAs and corticosterone synthesis in fatty tissues, thereby attenuates the delivery of corticosterone and FFAs to the liver. Collectively, this prevents high-fat diet-induced hepatic steatosis.
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http://dx.doi.org/10.2147/DDDT.S285828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178584PMC
May 2021

Unique structure of active platinum-bismuth site for oxidation of carbon monoxide.

Nat Commun 2021 Jun 7;12(1):3342. Epub 2021 Jun 7.

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

As the technology development, the future advanced combustion engines must be designed to perform at a low temperature. Thus, it is a great challenge to synthesize high active and stable catalysts to resolve exhaust below 100 °C. Here, we report that bismuth as a dopant is added to form platinum-bismuth cluster on silica for CO oxidation. The highly reducible oxygen species provided by surface metal-oxide (M-O) interface could be activated by CO at low temperature (~50 °C) with a high CO production rate of 487 μmol·g·s at 110 °C. Experiment data combined with density functional calculation (DFT) results demonstrate that Pt cluster with surface Pt-O-Bi structure is the active site for CO oxidation via providing moderate CO adsorption and activating CO molecules with electron transformation between platinum atom and carbon monoxide. These findings provide a unique and general approach towards design of potential excellent performance catalysts for redox reaction.
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http://dx.doi.org/10.1038/s41467-021-23696-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184822PMC
June 2021

Gold-iron selenide nanocomposites for amplified tumor oxidative stress-augmented photo-radiotherapy.

Biomater Sci 2021 Jun;9(11):3979-3988

Key Laboratory of Advanced Materials, Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China. and State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China.

The radio-resistance of tumor tissues has been considered a great challenge for cancer radiotherapy (RT).The development of nanoparticle (NP)-based radio-sensitizers can enhance the radio-sensitization of tumor tissues while reducing the side effects to surrounding tissues. However, most of the nano-radiosensitizers show increased radiation deposition with a high-Z element but achieve limited enhancement. Herein, we investigated polyethylene glycol (PEG)-modified gold-iron selenide nanocomposites (Au-FeSe2 NCs) for simultaneously enhancing therapeutic effects in multiple ways. In this study, the high-Z element Au (Z = 79) endows Au-FeSe2 NCs with enhanced X-ray deposition and thus causes more DNA damage. On the other hand, Au-FeSe2 exhibits the ability to produce reactive oxygen species (ROS) by catalyzing endogenous hydrogen peroxide in tumor sites as well as improve the hydrogen peroxide level during ionizing irradiation. Finally, combined with photothermal therapy (PTT), Au-FeSe2 NCs could exhibit a remarkable RT/PTT synergistic effect on tumor treatment.
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http://dx.doi.org/10.1039/d1bm00306bDOI Listing
June 2021

High-throughput biomonitoring of organophosphate flame-retardant metabolites in urine via 96-blade solid-phase microextraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry.

Talanta 2021 Sep 8;232:122466. Epub 2021 May 8.

Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada. Electronic address:

Organophosphate flame retardants (OPFRs) are widely used in consumer products and building materials, but their propensity for migration poses a problem with respect to polluting indoor environments, water, soil, and dust. OPFR metabolites in urine samples are appropriate biomarkers for assessing exposure risk levels. In this paper, a high-throughput method that couples 96-blade solid-phase microextraction with ultra-performance liquid chromatography-tandem mass spectrometry (SPME-UPLC-MS/MS) is applied for the simultaneous detection of four OPFR metabolites in urine samples. The results indicated that the best extraction was achieved using 96 blades coated with hydrophilic-lipophilic balance weak anion exchange (HLB-WAX). The proposed SPME method's extraction efficiency was maximized by optimizing extraction time, pH value, desorption solution, desorption volume, and desorption time, and it was validated in accordance with the Food and Drug Administration's guidelines. The findings indicated that the proposed method has a wide linearity range (0.5-100 ng mL) and low detection limits (0.09-0.14 ng mL). The method's accuracy ranged from 98% to 118%, with intra-day precision ranging from 1% to 10%. In contrast, inter-day precision ranged from 3% to 16%. Accuracy was also evaluated using independent urine samples, which ranged from 78% to 124% with corresponding relative standard deviations (1%-16%). Ultimately, DoCP was detected in two real samples at a concentration of 0.5-1.1 ng mL, and BEHP was detected at a concentration of 0.2-1.2 ng mL. Overall, the proposed SPME-UPLC-MS/MS method is reliable, accurate, and capable of simultaneously determining four OPFR metabolites in urine samples and screening them to assess exposure risk for humans.
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http://dx.doi.org/10.1016/j.talanta.2021.122466DOI Listing
September 2021

Chemical Stimulation of Rodent and Human Cortical Synaptosomes: Implications in Neurodegeneration.

Cells 2021 May 12;10(5). Epub 2021 May 12.

Department of Anatomy, University of Otago, Dunedin 9016, New Zealand.

Synaptic plasticity events, including long-term potentiation (LTP), are often regarded as correlates of brain functions of memory and cognition. One of the central players in these plasticity-related phenomena is the α-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor (AMPAR). Increased levels of AMPARs on postsynaptic membranes thus constitute a biochemical measure of LTP. Isolated synaptic terminals (synaptosomes) are an excellent ex vivo tool to monitor synaptic physiology in healthy and diseased brains, particularly in human research. We herein describe three protocols for chemically-induced LTP (cLTP) in synaptosomes from both rodent and human brain tissues. Two of these chemical stimulation protocols are described for the first time in synaptosomes. A pharmacological block of synaptosomal actin dynamics confirmed the efficiency of the cLTP protocols. Furthermore, the study prototypically evaluated the deficiency of cLTP in cortical synaptosomes obtained from human cases of early-onset Alzheimer's disease (EOAD) and frontotemporal lobar degeneration (FLTD), as well as an animal model that mimics FLTD.
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http://dx.doi.org/10.3390/cells10051174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151714PMC
May 2021

The Association of Morning Surge and Night-Time Dipping Blood Pressure with Significant and Complex Coronary Artery Lesions.

High Blood Press Cardiovasc Prev 2021 May 31. Epub 2021 May 31.

Department of Cardiology, Lanzhou University Second Hospital, 82 Cuiyingmen St, Lanzhou, 730030, Gansu, People's Republic of China.

Introduction: Hypertension responsible for more than 10 million deaths annually worldwide and abnormal diurnal blood pressure (BP) variation is associated with cardiovascular events.

Aim: This study aimed to investigate the association between the 24-h ambulatory night BP dipping and morning BP surge (MS) with characteristic of coronary artery lesions that may contribute cardiovascular events and mortality burden.

Methods: A cross sectional study over 1-year, collected 263 cases of hypertensive (80%) and non-hypertensive patients, aged 61 ± 10 years, who undergoing invasive coronary angiography (CAG) and 24-h ambulatory BP monitoring admitted to cardiology department complain of chest pain. The night-time/day-time dip and sleep-trough MS were calculated. Non-dipper status was considered when night-time/day-time dip < 10%, and significant coronary lesion (SCL) when ≥ 50 % stenosis in 1.5 mm vessels. The SYNTAX (Synergy Between Percutaneous Coronary Intervention with Taxus and Cardiac Surgery) score was used to quantify the complexity of SCL.

Result: The mean morning systolic BP (SBP) surge was higher in the high SYNTAX Score subgroup than low and intermediate subgroups (25 ± 11 vs 17 ± 13 and 10 ± 10 mmHg, p < 0.010). Non-dipper SBP status was more frequently in patients with SCL than non-SCL (p < 0.019). In ordinal regression, hypertension was independent predictor of SCL (odd ratio: 0.40, p < 0.003), the night-time/day-time BP dip was independent predictor of being in a higher SYNTAX score subgroup (systolic odd ratio: 0.88, diastolic odd ratio: 1.14 p < 0.05).

Conclusion: Hypertension is associated with SCL and the night-time/day-time BP dip as a continuous variable is associated with complex coronary lesion. Non-dipping as categorical variable and morning BP surge were not independent predictors of significant or complex coronary lesions.
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http://dx.doi.org/10.1007/s40292-021-00463-9DOI Listing
May 2021

Albuminuria and Dipstick Proteinuria for Predicting Mortality in Heart Failure: A Systematic Review and Meta-Analysis.

Front Cardiovasc Med 2021 13;8:665831. Epub 2021 May 13.

Department of Cardiology, Lanzhou University Second Hospital, Lanzhou, China.

Research suggest that albuminuria is not only an independent risk factor for the development of heart failure but may also act as a biomarker for predicting adverse outcomes. To date, no study has synthesized evidence on its role as a prognostic indicator. Thus, the current study aimed to quantitatively assess the prognostic utility of albuminuria as well as dipstick proteinuria in predicting mortality in heart failure patients. PubMed, Embase, ScienceDirect, CENTRAL, and Google Scholar databases were searched up to October 10, 2020. All studies reporting multivariable-adjusted hazard ratios (HR) for albuminuria or dipstick proteinuria for mortality and/or hospitalization in heart failure patients were included. Eleven studies were included. Seven assessed albuminuria and five assessed dipstick proteinuria. Our analysis revealed a statistically significant increased risk of all-cause mortality with microalbuminuria (HR: 1.54; 95% CI, 1.23-1.93; = 79%; = 0.0002) and macroalbuminuria (HR: 1.76; 95% CI, 1.21-2.56; = 88%; = 0.003) in heart failure patients. The risk of all-cause mortality and hospitalization was also significantly increased with macroalbuminuria. Microalbuminuria was associated with significantly increased cardiovascular mortality and combined cardiovascular mortality and hospitalization. Positive dipstick test for proteinuria was significantly associated with mortality in heart failure (HR: 1.54; 95% CI, 1.28-1.84; = 67%; < 0.00001). Both microalbuminuria and macroalbuminuria are predictors of mortality in patients with heart failure. Dipstick proteinuria may be used as a rapid screening test to predict mortality in these patients.
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http://dx.doi.org/10.3389/fcvm.2021.665831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155471PMC
May 2021

SERS substrate with wettability difference for molecular self-concentrating detection.

Nanotechnology 2021 May 28. Epub 2021 May 28.

School of Physics and Electronics, Shandong Normal University, Jinan, Shandong, CHINA.

The surface-enhanced Raman spectroscopy (SERS) has attracted much attention due to the powerful capability of quantificational analysis. Nowadays, most of the enhancement effect by SERS substrate is provided by the 'hot spots' occupying relatively small space. When the amount of analyte is too low, it is difficult to ensure that all the probe molecules can be placed into the 'hot spots', which is a headache in SERS quatification. In order to solve this problem, we have developed a structure of CuO nanowires/Ag nanoparticles with wettability capacity gradient, which can aggregate molecules in water and oil simultaneously under two different mechanisms of action. The limit of detection and enhancement factor of this structure are estimated as 10-15 M and 1.55×1011 respectively (for rhodamine 6G, R6G). Moreover, in the mimic sewage detection, it successfully realizes the spontaneous separation and aggregation of R6G molecules in aqueous solution and thiuram molecules in toluene, so as to achieve a high-effective and accurate Raman detection.
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http://dx.doi.org/10.1088/1361-6528/ac0665DOI Listing
May 2021

Two rice MYB transcription factors maintain male fertility in response to photoperiod by modulating sugar partitioning.

New Phytol 2021 May 25. Epub 2021 May 25.

Joint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Photoperiod-dependent male fertility is a critical enabler of modern hybrid breeding. A MYB transcription factor, CSA, is a key regulator of sugar partitioning in rice anthers, disruption of which causes photoperiod-sensitive male sterility. However, little is known about the molecular mechanisms governing plant fertility in response to photoperiod. Here, we have obtained another rice photoperiod-sensitive male sterile mutant, csa2, which exhibits semi-sterility under long-day (LD) conditions, with normal fertility under short-day (SD) conditions. CSA2 specifically expressed in anthers, and here is shown to be indispensable for sugar partitioning to anthers under LD conditions. The CSA2 protein can restore the fertility of csa mutants under SD conditions when expressed in a CSA-specific pattern, indicating that the two proteins share common downstream regulatory targets. Transcriptomic analyses also reveal discrete regulatory targets in anthers. Furthermore, the regulatory role of CSA2 in sugar transport was influenced by the photoperiod conditions during floral initiation, not simply during anther development. Collectively, we propose that rice evolved at least two MYB proteins, CSA2 and CSA, that regulate sugar transport in anthers under LD and SD conditions, respectively. This finding provides insight into the molecular mechanisms that regulate male fertility in response to photoperiod.
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http://dx.doi.org/10.1111/nph.17512DOI Listing
May 2021

Population Pharmacokinetic Analysis of Indacaterol/Glycopyrronium/Mometasone Furoate After Administration of Combination Therapies Using the Breezhaler Device in Patients with Asthma.

Eur J Drug Metab Pharmacokinet 2021 May 22. Epub 2021 May 22.

Pharmacokinetic Sciences, Novartis Institutes for Biomedical Research, Cambridge, USA.

Background And Objective: Clinical evidence suggests no clinically relevant pharmacokinetic interactions between indacaterol (IND), glycopyrronium (GLY) and mometasone furoate (MF). A population pharmacokinetic (popPK) analysis was conducted to identify structural models describing systemic pharmacokinetic profiles of IND, GLY and MF, and estimate the effect of covariates on their pharmacokinetics following inhalation as IND/GLY/MF.

Methods: Pharmacokinetic data from 698 patients with asthma were pooled from two Phase III studies that evaluated IND/MF medium- (150/160 µg) and high-dose (150/320 µg), IND/GLY/MF medium- (150/50/80 μg) and high-dose (150/50/160 μg), and a device bridging Phase II study with MF. One popPK model was developed each for IND, GLY and MF using a nonlinear mixed-effect modelling approach. Maximal and trough plasma concentrations were compared across formulations and studies, including data for IND/GLY from chronic obstructive pulmonary disease (COPD) patients. The effect of predefined covariates on the pharmacokinetics of components was evaluated using a full covariate modelling approach.

Results: The final pharmacokinetic models were two-compartment disposition models with first-order elimination and sequential zero-order/first-order absorption (IND), with bolus administration and first-order elimination (GLY), and with mixed zero-order/first-order absorption and first-order elimination (MF). All model parameters were estimated with good precision (% relative standard error: IND and MF ≤25%; GLY <10%). No clinically relevant covariate effect was observed on the pharmacokinetics of IND, GLY and MF. IND and GLY pharmacokinetic profiles were similar across different formulations.

Conclusion: Two-compartment popPK models adequately described the pharmacokinetics of IND, GLY and MF. The effect of covariates was not clinically relevant. The pharmacokinetic profiles of MF were comparable for combination products at corresponding medium- or high-dose inhaled corticosteroids. On a population level, the pharmacokinetics of IND and GLY were comparable between patients with asthma and COPD.
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http://dx.doi.org/10.1007/s13318-021-00689-xDOI Listing
May 2021

Ultrasonic scalpel combined with internal oxygen assisted sodium nitroprusside in laparoscopic cholecystectomy for elderly patients with acute cholecystitis.

Am J Transl Res 2021 15;13(4):3093-3101. Epub 2021 Apr 15.

Shanghai Tenth People's Hospital Shanghai 200072, China.

To analyze the application value of an ultrasonic scalpel combined with internal oxygen assisted intravenous drip of sodium nitroprusside in laparoscopic cholecystectomy for elderly patients with acute cholecystitis.

Methods: A total of 76 patients with acute cholecystitis who were admitted to our hospital from January 2017 to December 2018 were included and equally divided into two groups. The control group was treated with ultrasonic scalpel alone, while the study group was treated with ultrasonic scalpel combined with internal oxygen and intravenous drip of sodium nitroprusside. The incidence of postoperative adverse reactions, the recovery of surgical indicators, the quality of life score before and after treatment, and the changes of serum sICAM-1 and IL-6 expression were observed.

Results: The incidence of postoperative adverse reactions in the study group was significantly higher than that in the control group (P < 0.05); and the recovery of surgical indicators in the study group was better than that in the control group (P < 0.05). After treatment, the levels of serum sICAM-1 and IL-6 in the two groups showed a certain degree of decline, and the levels of serum sICAM-1 and IL-6 inflammatory factors in the study group were significantly lower than those in the control group (P < 0.05). After treatment, the scores of psychological state, physical health, independent ability, social relationship, surrounding environment and personal belief of the two groups were significantly higher than those before treatment (P < 0.05). After treatment, the scores of each field in the treatment group were significantly higher than that in the control group (P < 0.05).

Conclusion: It is safe and feasible to use an ultrasonic scalpel combined with internal oxygen to assist intravenous drip of sodium nitroprusside in the treatment of elderly patients with acute cholecystitis, but proper operation time and skilled operation are necessary.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129297PMC
April 2021

Clinical and Functional Characterization of Novel INSR Variants in Two Families With Severe Insulin Resistance Syndrome.

Front Endocrinol (Lausanne) 2021 29;12:606964. Epub 2021 Apr 29.

Department of Endocrinology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Objective: Defects in the insulin receptor () gene cause various severe insulin resistance conditions, including Donohue syndrome (DS), Rabson-Mendenhall syndrome (RMS) and type A insulin resistance (type A-IR). This study aimed to investigate the clinical characterization and molecular defects in three Chinese children with -related insulin resistance syndrome.

Methods: We reviewed the clinical data of three Chinese children with -related insulin resistance syndrome from two unrelated kindreds. Genetic analysis was performed using whole-exome sequencing and the effects of the novel variants were further assessed by functional assays.

Results: The proband with type A-IR presented with acanthosis nigricans, hypertrichosis, and euglycemia with mild insulin resistance in early childhood. His sister presented with features typical of type A-IR and was diagnosed with diabetes mellitus with severe insulin resistance at the age of 9.8 years. The proband with DS showed typical dysmorphic characteristics, severe intrauterine growth retardation, extreme insulin resistance, fasting hypoglycemia and postprandial hyperglycemia from birth. The heterozygote variants c.[3670G>A]; c.[3614C>T] were identified in both siblings with type A-IR; and c.[749_751del]; c.[3355C>T] in the patient with DS. studies showed that the novel variant c.749_751del [p.(Thr250del)] in the α-subunit, reduced expression of the mature INSR protein and severely impaired INSR function. In contrast, the novel variant c.3670G>A [p.(Val1224Met)] in the β-subunit had no effect on total protein expression and phosphorylation of INSR and Akt, suggesting that the variant p.Val1224Met appeared to be tolerated and was not responsible for the severe insulin resistance.

Conclusion: Our study detailed the clinical features of three patients with type A-IR and DS, and identified two novel variants in the gene. Functional assays indicated the novel variant p.Thr250del was pathogenic. In contrast, the novel variant p.Val1224Met was suggested to be tolerated by our experimental data, even though bioinformatics analyses predicted the variant as deleterious.
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http://dx.doi.org/10.3389/fendo.2021.606964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117416PMC
April 2021

Experimental study on preparation and anti-tumor efficiency of nanoparticles targeting M2 macrophages.

Drug Deliv 2021 Dec;28(1):943-956

Department of Biliary Surgery, West China Hospital of Sichuan University, Chengdu, China.

This study aimed to develop an effective therapy against M2 macrophages and to investigate the effects of imidazole and mannose modified carboxymethyl chitosan-nanoparticles (MIC-NPs) on tumor growth and antitumor immune responses. MIC-NPs were constructed and analyzed through H NMR, nano-laser particle size analyzer, and transmission electron microscopy. The nanoparticles were mainly distributed in 75-85 nm, and zeta potential was 1.5 mV. Cytotoxicity studies and indicated that MIC-NPs were safe. The targeting effect of MIC-NPs on M2 macrophages was observed through fluorescence microscope and microplate system. The results demonstrated the uptake of a large amount of FITC-loaded MIC-NPs by M2. Cell growth inhibition experiments showed that MIC-NPs significantly inhibited M2 through cell apoptosis. The evaluation of anti-tumor activity showed that MIC-NPs could accumulate in the tumor site to exert an anti-tumor effect. Flow cytometry showed that the proportion of M2 macrophages at the tumor site in the experimental group was significantly lower than that in the control group, while the Treg cells and cytotoxic T cells (CTL) were found to be increased. PCR detection showed that the cDNA of FIZZ, MR, TGF-β, and arginase, closely related to M2 macrophages, in the experimental group, was significantly lower than that in the control group, but there was no significant difference in the cDNA of Treg cell characteristic Foxp3 between the two groups. These results suggest that MIC-NPs are expected to provide a new and effective treatment for tumor.
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http://dx.doi.org/10.1080/10717544.2021.1921076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128207PMC
December 2021

Attenuation investigation influenced by the temperature and strain in an optical fiber composite low voltage cable.

Opt Express 2021 Apr;29(8):12696-12711

Optical fiber composite low voltage cable (OPLC) is an optimal way of connecting the smart grid with the load. In this paper, the field distribution of temperature and stress is simulated by applying the finite element method. At the overload condition, the fiber unit's temperature rises to 59°C that generates the strain of 387 µε. This strain and temperature together result in the additional attenuation of 0.053 dB/km in the optical fiber. Temperature and strain are termed as the major contributing factors towards the attenuation in the optical fiber as temperature caused 16.03% while strain caused 80.56% of the total loss.
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http://dx.doi.org/10.1364/OE.421716DOI Listing
April 2021

Ultra-high mechanical property and multi-layer porous structure of amidoximation ethylene-acrylic acid copolymer balls for efficient and selective uranium adsorption from radioactive wastewater.

Chemosphere 2021 Apr 30;280:130722. Epub 2021 Apr 30.

Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin, 150001, China; Institute of Advanced Marine Materials, Harbin Engineering University, 150001, China. Electronic address:

Adsorption uranium [U(VI)] from U-containing radioactive wastewater (URW) is a critical strategy for solving the resource shortage and environmental pollution in pace with the sustainable development of nuclear energy. However, the URW universally exhibits acidity and contains co-existing metal ions with high concentration. Herein, the amidoximation ethylene-acrylic acid copolymer balls (EAA-AO) with aciduric and super-high mechanical property were successfully synthesized through grafting diaminomaleonitrile and further treatment of amidoximation. Significantly, the mechanical properties of EAA-AO were not affected by the grafting process and maintained super-high mechanical properties. Furthermore, the -NH and unreacted -CN groups in diaminomaleonitrile adjusted the pK to make the optimal pH be 4. In addition, the microstructure of EAA-AO was transformed from the original dense to multi-layer porous structure, which promoted the mass transfer process and the contact between uranyl ions (UO) and internal adsorption active sites. The adsorption capacity of EAA-AO was about 1.78 times that of EAA at pH = 4, and the adsorption capacity for U(VI) was about 8.17 times that of Ba with the second highest adsorption capacity. Therefore, the EAA-AO exhibited ultra-high adsorption performance (q = 3.196 mg g) in the artificial radioactive wastewater, laying a good foundation for subsequent large-scale industrial adsorption of U(VI) in nuclear industrial wastewater.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130722DOI Listing
April 2021

Mitochondrial pathway of the lysine demethylase 5C inhibitor CPI-455 in the Eca-109 esophageal squamous cell carcinoma cell line.

World J Gastroenterol 2021 Apr;27(16):1805-1815

Department of Laboratory Medicine, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430079, Hubei Province, China.

Background: Esophageal cancer is a malignant tumor of the digestive tract that is difficult to diagnose early. CPI-455 has been reported to inhibit various cancers, but its role in esophageal squamous cell carcinoma (ESCC) is unknown.

Aim: To investigate the effects and mechanism of the lysine demethylase 5C inhibitor, CPI-455, on ESCC cells.

Methods: A methyl tetrazolium assay was used to detect the inhibitory effect of CPI-455 on the proliferation of Eca-109 cells. Apoptosis, reactive oxygen species (ROS), and mitochondrial membrane potential were assessed by flow cytometry. Laser confocal scanning and transmission electron microscopy were used to observe changes in Eca-109 cell morphology. The protein expression of P53, Bax, lysine-specific demethylase 5C (KDM5C), cleaved Caspase-9, and cleaved Caspase-3 were assayed by western blotting.

Results: Compared with the control group, CPI-455 significantly inhibited Eca-109 cell proliferation. Gemcitabine inhibited Eca-109 cell proliferation in a concentration- and time-dependent manner. CPI-455 caused extensive alteration of the mitochondria, which appeared to have become atrophied. The cell membrane was weakly stained and the cytoplasmic structures were indistinct and disorganized, with serious cavitation when viewed by transmission electron microscopy. The flow cytometry and western blot results showed that, compared with the control group, the mitochondrial membrane potential was decreased and depolarized in Eca-109 cells treated with CPI-455. CPI-455 significantly upregulated the ROS content, P53, Bax, Caspase-9, and Caspase-3 protein expression in Eca-109 cells, whereas KDM5C expression was downregulated.

Conclusion: CPI-455 inhibited Eca-109 cell proliferation mitochondrial apoptosis by regulating the expression of related genes.
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http://dx.doi.org/10.3748/wjg.v27.i16.1805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072195PMC
April 2021

Long-term safety, efficacy, and tolerability of levetiracetam in pediatric patients with epilepsy in Uygur, China: A retrospective analysis.

Epilepsy Behav 2021 Jul 5;120:108010. Epub 2021 May 5.

Department of Pharmacy, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Province 830001, China; Institute of Clinical Pharmacy, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang Province 830001, China. Electronic address:

Purpose: Levetiracetam is approved as an add-on therapy to treat refractory partial seizures in pediatric patients over four years old. The efficacy and safety in pediatric patients with epilepsy in Uygur, China, is unknown. Therefore, the objective of this study was to investigate the safety, efficacy, and tolerability of levetiracetam in pediatric patients with epilepsy in Uygur, China.

Methods: This retrospective study compared the efficacy and safety of levetiracetam monotherapy and in combination with other antiseizure medications (ASMs) in 1055 pediatric patients with epilepsy treated with levetiracetam. The seizure frequencies at 1, 2, and 3 years after starting levetiracetam therapy were recorded and compared with the baseline yearly frequency. Safety variables included the incidence and type of adverse reactions.

Results: A total of 680 (64%) pediatric patients responded to levetiracetam therapy with a more than 50% reduction in the frequency of seizures. Seizure-free rates increased over time, 13%, 15%, and 18% at 1, 2, and 3 years, respectively. The number of baseline ASMs and the order of levetiracetam introduction were highly significant, impacting the likelihood of seizure remission during a 3-year follow-up period (p < 0.001). During levetiracetam treatment, 233 pediatric patients (22%) experienced at least one adverse reaction.

Conclusion: These significant findings indicate that levetiracetam is likely to become a widely prescribed ASM for epilepsy in pediatric clinical practice because of its long-term safety, efficacy, and tolerability.
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http://dx.doi.org/10.1016/j.yebeh.2021.108010DOI Listing
July 2021

Intratympanic gentamicin injection for refractory ménière's disease (MD) has potential effect in preventing contralateral MD occurrence.

Acta Otolaryngol 2021 May 5:1-6. Epub 2021 May 5.

ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Background: Ménière's disease (MD), characterized by episodic vertigo attacks and fluctuating progressive hearing loss, is treated by low-dose intratympanic gentamicin (ITG) injections. Whether ITG causes hearing loss is controversial, and knowledge about its effects on the contralateral hearing and vestibular function is lacking.

Aims/objectives: We aimed to evaluate the effect of ITG on bilateral auditory and otolith organ function in patients with unilateral refractory MD.

Material And Methods: The data of 30 patients was collected, including history, and pure tone audiometry and vestibular-evoked myogenic potentials (VEMPs) results before and one month after ITG treatment. Changes in vertigo were assessed at a two-year follow-up.

Results: One month after ITG injection, auditory thresholds between 125 Hz and 8 kHz on the injection side remained unchanged but have improved on the contralateral side at 125 Hz, 250 Hz, 1 kHz. The cervical and ocular VEMP solicitation rates on the injection side were lower than before the injection. Two years after treatment, vertigo was improved in 88.5% and complete controlled in 76.7% patients respectively.

Conclusions And Significance: The intractable vertigo of MD can be effectively controlled by ITG injection. This can improve the low and medium frequency hearing level in the contralateral ear, suggesting that it might help prevent contralateral MD occurrence.
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http://dx.doi.org/10.1080/00016489.2021.1915499DOI Listing
May 2021

First Report of Fusarium ipomoeae Causing Peanut leaf Spot in China.

Plant Dis 2021 May 4. Epub 2021 May 4.

Shandong Peanut Research Institute , Plant Protect, No. 126 Wannianquan Road, Licang, Qingdao, Shandong, 266100, China, Qingdao, Shandong Province, China, 266100.

Peanut (Arachis hypogaea L.) is one of the most economically important crops as an important source of edible oil and protein. In August 2020, circular to oval-shaped brown leaf spots (2-6 mm in diameter) with well-defined borders surrounded by a yellow margin were observed on peanut plant leaves in Laixi City, Shandong Province, China. Symptomatic plants randomly distributed in the field, the incidence was approximately 5%. Leave samples were collected consisted of diseased tissue and the adjacent healthy tissue. The samples were dipped in a 70% (v/v) ethanol solution for 30 s and then soaked in a 0.1% (w/v) mercuric chloride solution for 60 s. The surface-sterilized tissues were then rinsed three times with sterile distilled water, dried and placed on Czapek Dox agar supplemented with 100 μg/ml of chloramphenicol. The cultures were incubated in darkness at 25 °C for 3-5 days. Fungal colonies were initially white and radial, turning to orange-brown in color, with abundant aerial mycelia. Macroconidia were abundant, 4 to 7 septate, with a dorsiventral curvature, and were 3.3-4.5 × 18.5-38.1 μm (n=100) in size; microconidia were absent; chlamydospores were produced in chains or clumps, ellipsoidal to subglobose, and thick walled. The morphological characteristics of the conidia were consistent with those of Fusarium spp. To identify the fungus, an EasyPure Genomic DNA Kit (TransGEN, Beijing, China) was used to extract the total genomic DNA from mycelia. The internal transcribed spacer region (ITS rDNA) and the translation elongation factor 1-α gene (TEF1) were amplified with primers ITS1/ITS4 (White et al. 1990) and EF1/EF2 (O'Donnell et al. 1998), respectively. Based on BLAST analysis, sequences of ITS (MT928727) and TEF1 (MT952337) showed 99.64% and 100% similarity to the ITS (MT939248.1), TEF1 (GQ505636.1) of F. ipomoeae isolates. Sequence analysis confirmed that the fungus isolated from the infected peanut was F. ipomoeae (Xia et al. 2019). The pathogenicity of the fungus was tested in the greenhouse. Twenty two-week-old peanut seedlings (cv. Huayu20) grown in 20-cm pots (containing autoclaved soil) were sprayed with a conidial suspension (105 ml-1) from a 15-day-old culture. Control plants were sprayed with distilled water. The experiment was conducted as a randomized complete block design, and placed at 25 °C under a 12-h photoperiod with 90% humidity. Symptoms similar to those in the field were observed on leaves treated with the conidial suspension ten days after inoculation, but not on control plants. F. ipomoeae was re-isolated from symptomatic leaves but not from the control plants. Reisolation of F. ipomoeae from inoculated plants fulfilled Koch's postulates. To our knowledge, this is the first report of F. ipomoeae causing peanut leaf spot in China. Our report indicates the potential spread of this pathogen in China and a systematic survey is required to develop effective disease management strategies.
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http://dx.doi.org/10.1094/PDIS-01-21-0226-PDNDOI Listing
May 2021

2D Honeycomb-Kagome Polymer Tandem as Effective Metal-Free Photocatalysts for Water Splitting.

Adv Mater 2021 May 4;33(21):e2008645. Epub 2021 May 4.

TU Dresden Fakultät für Chemie und Lebensmittelchemie, Bergstraße 66c, 01062, Dresden, Germany.

On the basis of first-principles calculations, the potential of applying 2D honeycomb-kagome polymers made of heteroatom-centered triangulene derivatives to photocatalyze water splitting is explored. The designed 2D polymers possess indirect bandgaps in the range of 1.80-2.84 eV and show pronounced light absorption in the ultraviolet and visible region of the solar spectrum. With suitable band edge alignment, the examined N- and B-center polymers can generate sufficient photon-excited electrons and holes to activate the hydrogen and oxygen evolution reactions, respectively. The combination of lattice-inherent band features (flat bands) with chemical functionalization (potential shift due to heteroatoms) makes it possible to construct tandem cells with suppressed electron/hole recombination for effective overall water splitting. In addition, there is a potential difference between the half-electrodes that can be utlized to power​ auxiliary components in self-sufficient photocatalyzers.
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http://dx.doi.org/10.1002/adma.202008645DOI Listing
May 2021

Long noncoding RNA CTD-2245E15.3 promotes anabolic enzymes ACC1 and PC to support non-small cell lung cancer growth.

Cancer Res 2021 May 3. Epub 2021 May 3.

Biochemistry, Nanjing University

Long noncoding RNAs (lncRNA) have been shown to play critical regulatory roles in the onset and progression of human cancers. However, the functions of a large proportion of lncRNAs are still unexplored. Here we describe a novel lncRNA, CTD-2245E15.3, that promotes lung tumorigenesis by regulating the anabolic enzymes acetyl-CoA carboxylase 1 (ACC1) and pyruvate carboxylase (PC). Differentially expressed lncRNAs between non-small cell lung cancer (NSCLC) and paired adjacent nontumor tissues were identified by a microarray and validated using quantitative real-time polymerase chain reaction. CTD-2245E15.3 was significantly upregulated in NSCLC and was mainly located in the cytoplasm. Knockdown of CTD-2245E15.3 by specific antisense oligonucleotides suppressed cell growth in vitro and in vivo, largely due to cell cycle arrest and induction of apoptosis. Overexpression of CTD-2245E15.3 in an orthotopic model of lung cancer led to a significant increase in total tumor burden. CTD-2245E15.3 exerted its oncogenic function by binding ACC1 and PC, which are key anabolic factors for biomolecule synthesis in rapidly proliferating tumor cells. Knockdown of CTD-2245E15.3 increased phosphorylation of ACC1 at an inhibitory site for enzymatic activity and promoted PC degradation via ubiquitination. Supplements of palmitate or oxaloacetate, products of ACC1 and PC, alleviated the suppression of cell growth caused by loss of CTD-2245E15.3. These findings reveal the important role of CTD-2245E15.3 as an oncogenic lncRNA in the anabolic process for tumor growth.
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http://dx.doi.org/10.1158/0008-5472.CAN-19-3806DOI Listing
May 2021

Bioactive skin-mimicking hydrogel band-aids for diabetic wound healing and infectious skin incision treatment.

Bioact Mater 2021 Nov 17;6(11):3962-3975. Epub 2021 Apr 17.

School of Chemistry, Xi'an Jiaotong University, Xi'an, 710049, China.

The treatment of diabetic chronic wounds remains a global challenge due to the up-regulated inflammation response, oxidant stress, and persistent infection during healing process. Developing wound dressing materials with ideal biocompatibility, adequate mechanical strength, considerable under-water adhesion, sufficient anti-inflammation, antioxidant, and antibacterial properties is on-demand for clinical applications. In this study, we developed a bioactive skin-mimicking hydrogel band-aid through the combination of tannic acid (TA) and imidazolidinyl urea reinforced polyurethane (PMI) (TAP hydrogel) and explored its potentials in various medical applications, including hemostasis, normal skin incision, full-thickness skin wounds, and bacterial-infection skin incision on diabetic mice. TA was loaded into PMI hydrogel network to enhance the mechanical properties of TAP hydrogels through multiple non-covalent interactions (break strength: 0.28-0.64 MPa; elongation at break: 650-930%), which could resist the local stress and maintain the structural integrity of wound dressings during applications. Moreover, owing to the promising moisture-resistant adhesiveness and organ hemostasis, outstanding anti-inflammation, antibacterial, and antioxidant properties, TAP hydrogels could efficiently promote the recovery of skin incision and defects on diabetic mice. To further simulate the practical situation and explore the potential in clinical application, we also verified the treatment efficiency of TAP hydrogel in -infected skin incision model on diabetic mice.
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http://dx.doi.org/10.1016/j.bioactmat.2021.04.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079829PMC
November 2021

Anti-bacterial and super-hydrophilic bamboo charcoal with amidoxime modified for efficient and selective uranium extraction from seawater.

J Colloid Interface Sci 2021 Sep 29;598:455-463. Epub 2021 Mar 29.

Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China; Institute of Advanced Marine Materials, Harbin Engineering University, 150001, China; Harbin Engineering University Capital Management Co. Ltd, Harbin 150001, China. Electronic address:

With the growing demand for nuclear energy, uranium extraction from seawater (UES) is becoming increasingly important due to the ocean reserves 4.5 billion tons for uranium(VI) [U(VI)]. Herein, two kinds of amidoxime modified bamboo charcoal (AOOBCS and AOOBCH) with porous structure, anti-bacterial, and super-hydrophilic properties were successfully synthetized by two etching methods (soaking and hydrothermal). The super-hydrophilic property of AOOBCH accelerated the contact between the amidoxime group and uranyl ions (UO), and promoted the action of anti-bacterial substances (bamboo-quinone) on bacteria to restrain the form of bacterial membrane. In addition, the amidoxime groups not only didn't destroy the super-hydrophilic surface, but also adjusted the adsorbents' pK by changing the amidoxime grafting rate. Under PH = 7, the adsorption capacity of AOOBCH was about 1.97 times that of AOOBCS and 2.95 times that of BC. Importantly, the AOOBCH exhibited ultra-high uptake capacity (6.37 mg g) and exceptional selectivity for U(VI) in 100-fold interfering ions simulated seawater system due to the chelation between C(NH)NOH and UO to form a more stable coordination structure (E = -36.56 eV). Benefiting from the superior performance and selectivity, the AOOBCH is a potential candidate for UES.
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http://dx.doi.org/10.1016/j.jcis.2021.03.154DOI Listing
September 2021