Publications by authors named "Jing Yu"

2,070 Publications

  • Page 1 of 1

Analysis in the influence factors of urethroplasty in DSD.

BMC Urol 2022 Aug 10;22(1):124. Epub 2022 Aug 10.

Department of Urology, National Center for Children's Health, Beijing Children's Hospital Affiliated to Capital Medical University, Beijing, 100045, China.

Background: At present, there is no specific research on the factors affecting the success rate of urethroplasty in patients with DSD. The purpose of this study is to explore the factors affecting the success of urethroplasty in DSD patients, and to provide some reference for the surgical treatment of DSD patients undergoing urethroplasty.

Method: We reviewed patients with DSD who underwent urethroplasty from January 2016 to December 2019 retrospectively. Patients were divided into four groups: the successful group, the urethrocutaneous fistula group, the urethral diverticulum group, and the urethral stricture group. Risk factors were determined from the following data included the DSD classification, the age of first operation, length of urethral defect, degree of hypospadias, cryptorchidism, micropenis, gonad type, hormone therapy before operation, transposition of penis and scrotum, surgical strategy, urethral covering material, and postoperative catheter removal time. We explored the difference of each factor between four groups through the comparative study of single factor and multifactor logistic regression analysis of related factors.

Result: 122 cases were enrolled in this group (n = 122), 12 cases were lost to follow-up. Median follow-up was 28 months (12-55 months).We found the success rate of operation decreased with longer urethral defect (B = - 0.473, P = 0.005). The success rate of operation was higher in staged operation and TPIT (TPIT = Transverse Preputial Island Tube operation)-related operation than primary operation (B = 1.238, P = 0.006) and TPIT-nonrelated operation (B = 2.293, P = 0.001). Although there was a significant difference between the age of the first operation and the occurrence of urethrocutaneous fistula (P = 0.006 < 0.05), there was no significant difference in logistic regression analysis (P = 0.161 > 0.05). The incidence of urethrocutaneous fistula was lower in TPIT-related operation than in TPIT-nonrelated operation (B = - 2.507, P = 0.000). The incidence of postoperative urethral diverticulum was lower in staged operation than in primary operation (B = - 1.737, P = 0.015).

Conclusion: For patients with disorder of sex development undergoing urethroplasty, the length of urethral defect is an independent risk factor affecting both the success rate of operation and the urethrocutaneous fistula. The age of the first operation has a statistically significant effect on the occurrence of postoperative urethrocutaneous fistula, but it is not an independent factor. Urethrocutaneous fistula is less found in TPIT-related operation in the study. Staged operation is an independent protective factor for postoperative urethral diverticulum compared with one-stage operation but isn't related to urethrocutaneous fistula.
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http://dx.doi.org/10.1186/s12894-022-01080-xDOI Listing
August 2022

Rho kinase inhibition on renal remodeling in an apatinib-induced hypertensive rat model.

J Hypertens 2022 Sep;40(9):1838-1840

Department of Cardiology, Lanzhou University Second Hospital, Lanzhou.

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http://dx.doi.org/10.1097/HJH.0000000000003184DOI Listing
September 2022

Combinatorial Strategies With PD-1/PD-L1 Immune Checkpoint Blockade for Breast Cancer Therapy: Mechanisms and Clinical Outcomes.

Front Pharmacol 2022 22;13:928369. Epub 2022 Jul 22.

Laboratory of Integrative Medicine, Clinical Research Center for Breast, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China.

As an emerging antitumor strategy, immune checkpoint therapy is one of the most promising anticancer therapies due to its long response duration. Antibodies against the programmed death-1 (PD-1) and programmed death ligand-1 (PD-L1) axis have been extensively applied to various cancers and have demonstrated unprecedented efficacy. Nevertheless, a poor response to monotherapy with anti-PD-1/PD-L1 has been observed in metastatic breast cancer. Combination therapy with other standard treatments is expected to overcome this limitation of PD-1/PD-L1 blockade in the treatment of breast cancer. In the present review, we first illustrate the biological functions of PD-1/PD-L1 and their role in maintaining immune homeostasis as well as protecting against immune-mediated tissue damage in a variety of microenvironments. Several combination therapy strategies for the combination of PD-1/PD-L1 blockade with standard treatment modalities have been proposed to solve the limitations of anti-PD-1/PD-L1 treatment, including chemotherapy, radiotherapy, targeted therapy, antiangiogenic therapy, and other immunotherapies. The corresponding clinical trials provide valuable estimates of treatment effects. Notably, several combination options significantly improve the response and efficacy of PD-1/PD-L1 blockade. This review provides a PD-1/PD-L1 clinical trial landscape survey in breast cancer to guide the development of more effective and less toxic combination therapies.
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http://dx.doi.org/10.3389/fphar.2022.928369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355550PMC
July 2022

High-throughput proteomic sample preparation using pressure cycling technology.

Nat Protoc 2022 Aug 5. Epub 2022 Aug 5.

Westlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, China.

High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling technology (PCT)-assisted sample preparation for proteomic analysis of biopsy tissues. A piece of fresh frozen or formalin-fixed paraffin-embedded tissue weighing ~0.1-2 mg is placed in a 150 μL pressure-resistant tube called a PCT-MicroTube with proper lysis buffer. After closing with a PCT-MicroPestle, a batch of 16 PCT-MicroTubes are placed in a Barocycler, which imposes oscillating pressure to the samples from one atmosphere to up to ~3,000 times atmospheric pressure. The pressure cycling schemes are optimized for tissue lysis and protein digestion, and can be programmed in the Barocycler to allow reproducible, robust and efficient protein extraction and proteolysis digestion for mass spectrometry-based proteomics. This method allows effective preparation of not only fresh frozen and formalin-fixed paraffin-embedded tissue, but also cells, feces and tear strips. It takes ~3 h to process 16 samples in one batch. The resulting peptides can be analyzed by various mass spectrometry-based proteomics methods. We demonstrate the applications of this protocol with mouse kidney tissue and eight types of human tumors.
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http://dx.doi.org/10.1038/s41596-022-00727-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362583PMC
August 2022

7-Methoxy-13-dehydroxypaxilline: New indole diterpenoid from an endophytic fungus sp. Nb 19.

Nat Prod Res 2022 Aug 5:1-9. Epub 2022 Aug 5.

Yunnan Key Laboratory of Southern Medicine Utilization, Yunnan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong, China.

ABSTACTA chemical investigation of the endophyte sp. Nb 19, isolated from leaves of the traditionally medical plant (Nees) Bremek., yielded one new indole diterpenoid, 7-methoxy-13-dehydroxypaxilline () together with seven known metabolites (-). The obtained structure of compound was elucidated by its spectroscopic data. In addition, the absolute configuration of compound was confirmed by ECD for the first time. Compounds - were evaluated for antitumor activity against MCF-7, HepG2, and HCCC-9810 cell lines.
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http://dx.doi.org/10.1080/14786419.2022.2107639DOI Listing
August 2022

[The Tertiary Hospital's Medical Materials Supply in the Prevention of Public Health Emergencies].

Zhongguo Yi Liao Qi Xie Za Zhi 2022 Jul;46(4):469-472

Northern Jiangsu People's Hospital, Yangzhou, 225001.

Objective: To ensure the supply of prevention materials in the tertiary public hospitals in prefecturelevel cities, and to make the process of allocating prevention materials more scientific and reasonable.

Methods: Open the green passage, simplify the procurement process, carry out emergency procurement of related materials, ensure timely delivery of prevention materials, distribute them at different levels, and strengthen the warehouse management of prevention materials.

Results: The scheme of emergancy supplies was constantly improved, and the supply of prevention materials was completed with good quality.

Conclusions: Using scientific and efficient management methods, the supply of prevention materials in medical institutions has been guaranteed, which has experience and reference significance for the prevention and control of similar public health emergencies in the future.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2022.04.024DOI Listing
July 2022

Bone marrow Relative Biological Effectiveness (RBE) for a Pb-labeled anti-HER2/neu antibody.

Int J Radiat Oncol Biol Phys 2022 Aug 1. Epub 2022 Aug 1.

Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States.

Purpose: We have determined the in vivo relative biological effectiveness (RBE) of an alpha-particle emitting radiopharmaceutical therapeutic (αRPT) agent (Pb-labeled anti-HER-2/neu antibody) for the bone marrow, a potentially dose-limiting normal tissue.

Methods And Materials: The RBE was measured in mice using femur marrow cellularity as the biological endpoint. External beam radiotherapy (EBRT), delivered by a small animal radiation research platform was used as the reference radiation. Alpha-particle emissions were delivered by Bi, following the decay of its parent nuclide Pb, conjugated onto an anti-HER2/neu antibody. The alpha-particle absorbed dose to the marrow following intravenously administered (tail vein) 122.1-921.3 kBq Pb-TCMC-7.16.4 was calculated. Mice were sacrificed at 0-7 days post-treatment (dpt) and femur bone marrow was counted for radioactivity. Changes in marrow cellularity were assessed by histopathology.

Results: The dose-response for EBRT and Pb-anti-HER2/neu antibody were linear-quadratic and linear, respectively. By transforming the EBRT dose-response relationship into a linear relationship using the EQD2 formalism we obtained an RBE (denoted RBE2) of 6.4; this value is independent of cellularity and absorbed dose.

Conclusions: Since hematologic toxicity is dose-limiting in almost all antibody-based RPT, in vivo measurements of RBE are important in helping identify an initial administered activity (AA) in phase 1 escalation trials. Applying the RBE2 and assuming typical antibody clearance kinetics (Biological half-life of 48 h) with a modified blood-based dosimetry method, an average AA of approximately 185.5 MBq (5.0 mCi) per patient could be administered before hematologic toxicity is anticipated.
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http://dx.doi.org/10.1016/j.ijrobp.2022.07.1842DOI Listing
August 2022

Biodegradable MnO-Based Nanoparticles with Engineering Surface for Tumor Therapy: Simultaneous Fenton-Like Ion Delivery and Immune Activation.

ACS Nano 2022 Aug 4. Epub 2022 Aug 4.

Beijing Key Laboratory for Magnetoelectric Materials and Devices, School of Materials Science and Engineering, Peking University, Beijing 100871, China.

Immune checkpoint inhibitors have achieved significant clinical success but are still suffering from inadequate immune activation. It is worth noting that manganese as a nutritional inorganic trace element is closely associated with immune activation to fight against tumor growth and metastasis the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway. Herein, we designed hollow mesoporous silica-coated MnO nanoparticles (NPs), followed by conjugation of tumor homing peptide iRGD (CRGDKGPD). The obtained NPs ([email protected] NPs) were applied to magnetic resonance imaging (MRI)-guided tumor immune-chemodynamic combination therapy, in which MnO NPs can be harnessed for cGAS-STING pathway-activated immunotherapy, Fenton-like reaction-induced reactive oxygen species upregulation, and T-weighted MRI. The rough surface and large cavities of the mSiO shell promote cellular uptake and MnO NPs delivery. Meanwhile, it was found that [email protected] NPs would dissociate under an acid environment, resulting in tumor specificity of MRI and exogenous Mn release. Our results revealed that these pH-responsive biodegradable [email protected] NPs synergized with α-PD-1 (PD-1 = programmed cell death-1) blocking antibody to highly elicit cytotoxic T lymphocyte infiltration and restrict melanoma progression and metastasis, which were envisioned as a promising candidate for tumor theranostics.
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http://dx.doi.org/10.1021/acsnano.2c00969DOI Listing
August 2022

The Whole-transcriptome Landscape of Diabetes-related Sarcopenia Reveals the Specific Function of Novel lncRNA Gm20743.

Commun Biol 2022 Aug 1;5(1):774. Epub 2022 Aug 1.

Department of Geriatrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

While the exact mechanism remains unclear, type 2 diabetes mellitus increases the risk of sarcopenia which is characterized by decreased muscle mass, strength, and function. Whole-transcriptome RNA sequencing and informatics were performed on the diabetes-induced sarcopenia model of db/db mice. To determine the specific function of lncRNA Gm20743, the detection of Mito-Sox, reactive oxygen species, Ethynyl-2'-deoxyuridine, and myosin heavy chain was performed in overexpressed and knockdown-Gm20743 C2C12 cells. RNA-seq data and informatics revealed the key lncRNA-mRNA interactions and indicated a potential regulatory role of lncRNAs. We characterized three core candidate lncRNAs Gm20743, Gm35438, 1700047G03Rik, and their potential function. Furthermore, the results suggested lncRNA Gm20743 may be involved in regulating mitochondrial function, oxidative stress, cell proliferation, and myotube differentiation in skeletal muscle cells. These findings significantly improve our understanding of lncRNAs that may mediate muscle mass, strength, and function in diabetes and represent potential therapeutic targets for diabetes-induced sarcopenia.
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http://dx.doi.org/10.1038/s42003-022-03728-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343400PMC
August 2022

Single-Cell Metabolomics in Hematopoiesis and Hematological Malignancies.

Front Oncol 2022 13;12:931393. Epub 2022 Jul 13.

Laboratory of Integrative Medicine, Clinical Research Center for Breast, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China.

Aberrant metabolism contributes to tumor initiation, progression, metastasis, and drug resistance. Metabolic dysregulation has emerged as a hallmark of several hematologic malignancies. Decoding the molecular mechanism underlying metabolic rewiring in hematological malignancies would provide promising avenues for novel therapeutic interventions. Single-cell metabolic analysis can directly offer a meaningful readout of the cellular phenotype, allowing us to comprehensively dissect cellular states and access biological information unobtainable from bulk analysis. In this review, we first highlight the unique metabolic properties of hematologic malignancies and underscore potential metabolic vulnerabilities. We then emphasize the emerging single-cell metabolomics techniques, aiming to provide a guide to interrogating metabolism at single-cell resolution. Furthermore, we summarize recent studies demonstrating the power of single-cell metabolomics to uncover the roles of metabolic rewiring in tumor biology, cellular heterogeneity, immunometabolism, and therapeutic resistance. Meanwhile, we describe a practical view of the potential applications of single-cell metabolomics in hematopoiesis and hematological malignancies. Finally, we present the challenges and perspectives of single-cell metabolomics development.
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http://dx.doi.org/10.3389/fonc.2022.931393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326066PMC
July 2022

Nicotinamide Mononucleotide Ameliorates Cellular Senescence and Inflammation Caused by Sodium Iodate in RPE.

Oxid Med Cell Longev 2022 18;2022:5961123. Epub 2022 Jul 18.

Department of Ophthalmology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200072, China.

Senescent cells have been demonstrated to have lower cellular NAD levels and are involved in the development of various age-related diseases, including age-related macular degeneration (AMD). Sodium iodate (NaIO) has been primarily used as an oxidant to establish a model of dry AMD. Results of previous studies have showed that NaIO induced retinal tissue senescence . However, the role of NaIO and the mechanism by which it induces retinal pigment epithelium (RPE) senescence remains unknown. In this study, RPE cell senescence was confirmed to be potentially induced by NaIO. The results showed that the number of senescence-associated--galactosidase (SA--gal-)-positive cells and the protein levels of p16 and p21 increased after NaIO treatment. Additionally, the senescent RPE cells underwent oxidative stress and NAD depletion. Furthermore, significant DNA damage and mitochondrial dysfunction were also detected in senescent RPE cells. The antioxidant N-acetylcysteine (NAC) could alleviate cellular senescence only by a minimal degree, whereas supplementation with nicotinamide mononucleotide (NMN) strongly ameliorated RPE senescence through the alleviation of DNA damage and the maintenance of mitochondrial function. The protective effects of NMN were demonstrated to rely on undisturbed Sirt1 signaling. Moreover, both the expression of senescence markers of RPE and subretinal inflammatory cell infiltration were decreased by NMN treatment . Our results indicate that RPE senescence induced by NaIO acquired several key features of AMD. More importantly, NMN may potentially be used to treat RPE senescence and senescence-associated pre-AMD changes by restoring the NAD levels in cells and tissues.
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http://dx.doi.org/10.1155/2022/5961123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313989PMC
July 2022

Tea Polyphenols as Prospective Natural Attenuators of Brain Aging.

Nutrients 2022 Jul 22;14(15). Epub 2022 Jul 22.

Department of Food Science and Engineering, Ningbo University, Ningbo 315211, China.

No organism can avoid the process of aging, which is often accompanied by chronic disease. The process of biological aging is driven by a series of interrelated mechanisms through different signal pathways, including oxidative stress, inflammatory states, autophagy and others. In addition, the intestinal microbiota play a key role in regulating oxidative stress of microglia, maintaining homeostasis of microglia and alleviating age-related diseases. Tea polyphenols can effectively regulate the composition of the intestinal microbiota. In recent years, the potential anti-aging benefits of tea polyphenols have attracted increasing attention because they can inhibit neuroinflammation and prevent degenerative effects in the brain. The interaction between human neurological function and the gut microbiota suggests that intervention with tea polyphenols is a possible way to alleviate brain-aging. Studies have been undertaken into the possible mechanisms underpinning the preventative effect of tea polyphenols on brain-aging mediated by the intestinal microbiota. Tea polyphenols may be regarded as potential neuroprotective substances which can act with high efficiency and low toxicity.
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http://dx.doi.org/10.3390/nu14153012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332553PMC
July 2022

Quality Evaluation of the Oil of spp.

Foods 2022 Jul 26;11(15). Epub 2022 Jul 26.

Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.

The oil of spp. has become a well-known high-quality edible oil because of its rich nutrition. It is of great significance to breed fine varieties of spp. for the sustainable growth of the spp. industry. This study mainly evaluated the quality and antioxidant capacity of the camellia seed from several sources. The fatty acid composition and main active components of 40 kinds of , , , and seeds, and so on, from different regions, were tested using GC-MS and HPLC. The quality of different spp. germplasm resources was comprehensively evaluated using multiple indices. The unsaturated fatty acid content and the antioxidant capacity of from Hainan were higher than those of Abel. In addition, there were a few differences in the fatty acid compositions of spp. oil from different species. Correlation analysis confirmed that rutin, total saponin, total flavonoids, squalene, and vitamin E were strongly correlated to the antioxidant capacity of spp. In the comprehensive evaluation, the best quality and strongest antioxidant activity were found for Chengmai Dafeng (). These methods in the study were applied for the first time for the quality evaluation of the Camellia spp. species. This study provided new insights into the quality evaluation of the spp. species, thus facilitating further development of variety breeding along with quality evaluation.
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http://dx.doi.org/10.3390/foods11152221DOI Listing
July 2022

Effect of Flaxseed Supplementation on Milk and Plasma Fatty Acid Composition and Plasma Parameters of Holstein Dairy Cows.

Animals (Basel) 2022 Jul 26;12(15). Epub 2022 Jul 26.

Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

The objective of this study was to determine the effect of whole flaxseed and ground flaxseed supplementation on the composition of fatty acids in plasma and milk, particularly the content of omega-3 polyunsaturated fatty acids (n-3 PUFAs). Thirty Holstein dairy cows were randomly assigned to three treatment groups. Cows were fed a total mixed ration without flaxseed (CK), 1500 g of whole flaxseed (WF), and 1500 g of ground flaxseed (GF) supplementation. There were no differences observed in dry matter intake, milk yield, energy-corrected milk, and 4% fat-corrected milk ( > 0.05). Compared with the CK group, the contents of α-linolenic acid (ALA), eicosatrienoic acid, and eicosapentaenoic acid increased in the plasma and milk WF and GF groups, and the content of docosahexaenoic acid and total n-3 PUFA was higher in GF than the other groups ( < 0.001). The ALA yield increased to 232% and 360% in WF and GF, respectively, compared to the CK group. Compared with the WF group, GF supplementation resulted in an increased milk ALA/ALA intake ratio ( < 0.001). Flaxseed supplementation increased the activity of GSH-Px and decreased the concentration of MDA in milk ( < 0.001). Plasma parameters did not differ among the treatments ( > 0.05). This result indicated that compared with the WF group, GF supplementation in the diet showed higher efficiency in increasing the total n-3 PUFA levels and the milk ALA/ALA intake ratio, and decreased the ratio of n-6 PUFAs to n-3 PUFAs in milk.
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http://dx.doi.org/10.3390/ani12151898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332015PMC
July 2022

Development of Certified Reference Materials for the Determination of Apparent Amylose Content in Rice.

Molecules 2022 Jul 21;27(14). Epub 2022 Jul 21.

China National Rice Research Institute, Hangzhou 310006, China.

Apparent amylose content (AAC) is one of the most important parameters in rice quality evaluation. In this study, four rice reference materials used to test rice AAC were developed. The AAC of rice reference materials were measured by a spectrophotometric method with a defatting procedure, calibrated from potato amylose and waxy rice amylopectin at the absorption wavelengths of 620 and 720 nm. Homogeneity test ( = 20) was judged by F-test based on the mean squares of among and within bottles, and short- and long-term stability monitoring was performed by T-test to check if there was significant degradation at the delivery temperature of under 40 °C (14 days) and at 0-4 °C storage condition (18 months), respectively. After joint evaluation by ten laboratories, Dixion and Cochran statistical analyses were presented. The expanded uncertainties were calculated based on the uncertainty of homogeneity, short- and long-term stability, and inter-laboratory validation containing factor = 2. It found that the four reference materials were homogenous and stable, and had the AAC (g/100 g, = 2) of 2.96 ± 1.01, 10.68 ± 0.66, 17.18 ± 1.04, and 16.09 ± 1.29, respectively, at 620 nm, and 1.46 ± 0.49, 10.44 ± 0.56, 16.82 ± 0.75, and 24.33 ± 0.52, respectively, at 720 nm. It was indicated that 720 nm was more suitable for the determination of rice AAC with lower uncertainties. The determinations of the AAC of 11 rice varieties were carried out by two methods, the method without defatting and with calibration from the four rice reference materials and the method with a defatting procedure and calibrating from potato amylose and waxy rice amylopectin. It confirmed that the undefatted rice reference materials could achieve satisfactory results to test the rice samples with the AAC ranging from 1 to 25 g/100 g. It would greatly reduce the time cost and improve testing efficiency and applicability, and provide technical support for the high-quality development of the rice industry.
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http://dx.doi.org/10.3390/molecules27144647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322866PMC
July 2022

Effects of Electromyography Bridge on Upper Limb Motor Functions in Stroke Participants: An Exploratory Randomized Controlled Trial.

Brain Sci 2022 Jun 30;12(7). Epub 2022 Jun 30.

Department of Rehabilitation Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

The electromyography bridge (EMGB) plays an important role in promoting the recovery of wrist joint function in stroke patients. We investigated the effects of the EMGB on promoting the recovery of upper limb function in hemiplegia. Twenty-four stroke patients with wrist dorsal extension dysfunction were recruited. Participants were randomized to undergo EMGB treatment or neuromuscular electrical stimulation (NMES). Treatments to wrist extensors were conducted for 25 min, twice a day, 5 days per week, for 1 month. Outcome measures: active range of motion (AROM) of wrist dorsal extension; Fugl-Meyer assessment for upper extremity (FMA-UE); Barthel index (BI); and muscle strength of wrist extensors. After interventions, patients in the NMES group had significantly greater improvement in the AROM of wrist dorsal extension at the 4th week and 1st month follow-up ( < 0.05). However, patients in the EMGB group had a statistically significant increase in AROM only at the follow-up assessment. No significant differences were observed in the AROM between the EMGB group and the NMES group ( > 0.05). For secondary outcomes in the EMGB group, compared to baseline measurements, FMA-UE, BI, extensor carpi radialis and extensor carpi ulnaris muscle strength were significantly different as early as the 4th week ( < 0.05). The muscle strength of the extensor digitorum communis muscle showed significant differences at the follow-up ( < 0.05). There were no statistically significant differences between patients in the two groups in any of the parameters evaluated ( > 0.05). The combination of EMGB or NMES with conventional treatment had similar effects on the improvement of the hemiplegic upper limb as assessed by wrist dorsal extension, FMA-UE, and activities of daily living. The improvement in both groups was maintained until 1 month after the intervention.
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http://dx.doi.org/10.3390/brainsci12070870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312916PMC
June 2022

UFMylation System: An Emerging Player in Tumorigenesis.

Cancers (Basel) 2022 Jul 19;14(14). Epub 2022 Jul 19.

Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200025, China.

Ubiquitin-fold modifier 1 (UFM1), a newly identified ubiquitin-like molecule (UBLs), is evolutionarily expressed in multiple species except yeast. Similarly to ubiquitin, UFM1 is covalently attached to its substrates through a well-orchestrated three-step enzymatic reaction involving E1, the UFM1-activating enzyme (ubiquitin-like modifier-activating enzyme 5, UBA5); E2, the UFM1-conjugating enzyme 1 (UFC1); and E3, the UFM1-specific ligase 1 (UFL1). To date, numerous studies have shown that UFM1 modification is implicated in various cellular processes, including endoplasmic reticulum (ER) stress, DNA damage response and erythroid development. An abnormal UFM1 cascade is closely related to a variety of diseases, especially tumors. Herein, we summarize the process and functions of UFM1 modification, illustrating the relationship and mechanisms between aberrant UFMylation and diversified tumors, aiming to provide novel diagnostic biomarkers or therapeutic targets for cancer treatments.
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http://dx.doi.org/10.3390/cancers14143501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323365PMC
July 2022

Advances in Single-Cell Toxicogenomics in Environmental Toxicology.

Environ Sci Technol 2022 Jul 26. Epub 2022 Jul 26.

State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, PR China.

The toxicity evaluation system of environmental pollutants has undergone numerous changes due to the application of new technologies. Single-cell toxicogenomics is rapidly changing our view on environmental toxicology by increasing the resolution of our analysis to the level of a single cell. Applications of this technology in environmental toxicology have begun to emerge and are rapidly expanding the portfolio of existing technologies and applications. Here, we first summarized different methods involved in single-cell isolation and amplification in single-cell sequencing process, compared the advantages and disadvantages of different methods, and analyzed their development trends. Then, we reviewed the main advances of single-cell toxicogenomics in environmental toxicology, emphatically analyzed the application prospects of this technology in identifying the target cells of pollutants in early embryos, clarifying the heterogeneous response of cell subtypes to pollutants, and finding pathogenic bacteria in unknown microbes, and highlighted the unique characteristics of this approach with high resolution, high throughput, and high specificity by examples. We also offered a prediction of the further application of this technology and the revolution it brings in environmental toxicology. Overall, these advances will provide practical solutions for controlling or mitigating exogenous toxicological effects that threaten human and ecosystem health, contribute to improving our understanding of the physiological processes affected by pollutants, and lead to the emergence of new methods of pollution control.
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http://dx.doi.org/10.1021/acs.est.2c01098DOI Listing
July 2022

Global trends and prospects of blue carbon sinks: a bibliometric analysis.

Environ Sci Pollut Res Int 2022 Jul 26. Epub 2022 Jul 26.

College of Oceanic and Atmospheric Sciences, Ocean University of China, Qingdao, 266100, People's Republic of China.

Blue carbon sinks (mangroves, saltmarshes, and seagrasses) are considered an effective nature-based approach for climate change mitigation. Despite growing interest, a systematic review of this topic is still scarce. This study evaluated 1348 blue carbon sink-related articles from 1990 to 2020 using bibliometric technology. The results from total of 85 countries, 1538 institutions, and 4492 authors indicated that blue carbon sink research shows the characteristics of rapid growth. The most active country, institution, and author were USA, Chinese Academy of Sciences, and Duarte C.M., respectively. Relatively close academic collaboration has formed in blue carbon science. Environmental Sciences was the most popular category with 590 papers. The percentages of articles related to mangroves, saltmarshes, and seagrasses were 63.87%, 40.36%, and 40.65%, respectively. Mangrove carbon sinks are the most popular topic, and stable isotope and remote sensing are the most researched technologies for mapping and quantifying blue carbon sinks. The threats to blue carbon sinks are complex and distinctive. Restoration, conservation, and management of blue carbon ecosystems aimed to improve their carbon sink capacity are becoming hot issues and should be further investigated in the future. These findings provide a scientific roadmap for further research in this field and will enable stakeholders to identify the research trend.
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http://dx.doi.org/10.1007/s11356-022-22216-4DOI Listing
July 2022

MRI-Based Optimization Design of the Pre-Spinal Route of Contralateral C7 Nerve Transfer for Spastic Arm Paralysis.

Front Surg 2022 29;9:837872. Epub 2022 Jun 29.

Department of Radiology, Jing'an District Central Hospital, Shanghai, China.

Purpose: The prespinal route of contralateral cervical 7 nerve transfer developed by Prof. Wendong Xu helps realize the direct anastomosis of the bilateral cervical 7 nerves. However, 20% of operations still require a nerve graft, which leads to an unfavorable prognosis. This study aims to explore the optimized prespinal route with MRI to further improve the prognosis.

Methods: The current study enrolled 30 patients who suffered from central spastic paralysis of an upper limb and who underwent contralateral cervical 7 nerve transfer via Prof. Xu's prespinal route through the anterior edge of the contralateral longus colli. MRI images were used to analyze the route length, vertebral artery exposure, and contralateral cervical 7 nerve included angle. Three prespinal routes were virtually designed and analyzed. The selected optimal route was applied to another 50 patients with central spastic paralysis of an upper limb for contralateral cervical 7 nerve transfer.

Results: By the interventions on the 30 patients, the middle and posterior routes were shorter than the anterior route in length, but with no statistical difference between the two routes. Of 30 contralateral vertebral arteries, 26 were located at the posterior medial edge of the longus colli. The average included angles of the anterior, middle, and posterior routes were 108.02 ± 7.89°, 95.51 ± 6.52°, and 72.48 ± 4.65°, respectively. According to these data, the middle route was optimally applied to 50 patients, in whom the rate of nerve transplantation was only 4%, and no serious complications such as vertebral artery or brachial plexus injury occurred.

Conclusion: The low rate of nerve transplantation in 50 patients and the absence of any serious complications in these cases suggests that the middle route is the optimal one.
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http://dx.doi.org/10.3389/fsurg.2022.837872DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276985PMC
June 2022

Targeting the lncRNA FGD5-AS1/miR-497-5p/PD-L1 Axis Inhibits Malignant Phenotypes in Colon Cancer (CC).

Biomed Res Int 2022 6;2022:1133332. Epub 2022 Jul 6.

Molecular Diagnosis Center of Yunnan Cancer Center, The Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital), Kunzhou Road No. 519, Kunming City, 650100 Yunnan Province, China.

Long noncoding RNAs (lncRNAs) regulate cancer progression and drug resistance. However, the role of lncRNA FGD5-AS1 in regulating colon cancer (CC) progression is still largely unknown. Hence, this study investigated the role of lncRNA FGD5-AS1 in regulating colon cancer (CC) progression and found that lncRNA FGD5-AS1 regulated miR-497-5p/PD-L1 axis to promote cancer progression in CC cells and . Specifically, we found that lncRNA FGD5-AS1 and PD-L1 tended to be high-expressed, while miR-497-5p was low-expressed in CC tissues and cell lines compared to the normal adjacent tissues and cells. Next, we found that lncRNA FGD5-AS1 positively regulated PD-L1 in CC cells by sponging miR-497-5p. Finally, our gain- and loss-of-function experiments evidenced that the lncRNA FGD5-AS1/miR-497-5p/PD-L1 axis regulates CC progression. Functionally, the data suggested that lncRNA FGD5-AS1 positively regulated while miR-497-5p negatively modulated malignant phenotypes, including cell proliferation, viability, invasion, migration, epithelial-mesenchymal transition (EMT), and tumorigenesis in CC cells. Interestingly, the inhibiting effects of lncRNA FGD5-AS1 ablation on CC development were abrogated by both silencing miR-497-5p and upregulating PD-L1. This study found that lncRNA FGD5-AS1 sponged miR-497-5p to upregulate PD-L1, resulting in CC progression, and provided novel agents for CC diagnosis and prognosis.
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http://dx.doi.org/10.1155/2022/1133332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279048PMC
July 2022

Nanoparticle-Based Combination Therapy for Melanoma.

Front Oncol 2022 28;12:928797. Epub 2022 Jun 28.

Department of Plastic and Cosmetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Melanoma is a cutaneous carcinoma, and its incidence is rapidly increasing with every year. The treatment options for melanoma have been comprehensively studied. Conventional treatment methods (e.g., radiotherapy, chemotherapy and photodynamic therapy) with surgical removal inevitably cause serious complications; moreover, resistance is common. Nanoparticles (NPs) combined with conventional methods are new and promising options to treat melanoma, and many combinations have been achieving good success. Due to their physical and biological features, NPs can help target intended melanoma cells more efficiently with less damage. This creates new hope for a better treatment strategy for melanoma with minimum damage and maximum efficacy.
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http://dx.doi.org/10.3389/fonc.2022.928797DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273962PMC
June 2022

Episodic memory updating among older adults: moderating role of prior knowledge.

Memory 2022 Jul 14:1-8. Epub 2022 Jul 14.

Faculty of Psychology, Southwest University, Chongqing, People's Republic of China.

Memory updating is an adaptive function that requires people's registry of changes of episodes. However, the research on the role of prior knowledge on memory updating among older adults is scant. We instructed young and older adults to learn two sets of pairs with overlapping scene (A) on Day 1 (A-B) and Day 2 (A-C) and tested the competing memories on Day 3. We further manipulated the schema-congruency between item (B/C) and scene (A). Young adults performed comparatively well in the A-B and A-C memory tests, and showed no difference under different congruency conditions. However, memory updating among older adults was moderated by prior knowledge, with better memory performance in A-C test relative to A-B test when the to-be-updated item C was schema-congruent, however, with poorer memory performance in the A-C test when the to-be-updated item is schema-incongruent. This study advances the understanding that prior knowledge significantly contributes to memory updating among older adults. They would experience retroactive interference when the to-be-updated memories were consistent with their prior knowledge, yet proactive interference when the to-be-updated memories were inconsistent with their prior knowledge. Meanwhile, prior knowledge among young adults does not affect memory updating, given that their memory patterns are consistent across congruency conditions.
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http://dx.doi.org/10.1080/09658211.2022.2099901DOI Listing
July 2022

LncRNA Meg3 knockdown reduces corneal neovascularization and VEGF-induced vascular endothelial angiogenesis via SDF-1/CXCR4 and Smad2/3 pathway.

Exp Eye Res 2022 Jul 9;222:109166. Epub 2022 Jul 9.

Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai, China; Department of Ophthalmology, The Third People's Hospital of Bengbu, Bengbu, China. Electronic address:

The crucial effect of vascular endothelial growth factor (VEGF)-induced vascular angiogenesis has been well known in corneal neovascularization (CNV). This research aimed to determine the underlying value and mechanism of Meg3 on CNV in vivo and in vitro. In an alkali-burned mouse model, length and area of new vessels were increased along with thinning of corneal epithelium, accompanied by the overexpression of Meg3. Notably, subconjunctival injection of shMeg3 suppressed the degree of injury in cornea, causing expression of the angiogenesis markers--VEGF-A and CD31 decreased. In VEGF-induced human umbilical vein endothelial cells (HUVECs), knockdown of Meg3 antagonized the enhancement of viability, proliferation, wound healing ability and angiogenesis by VEGF. The proteins expression of VEGF-A, CD31, SDF-1/CXCR4 as well as phosphoraylation-Smad2/3 pathways, which were related to angiogenesis, were reduced with Meg3 deficiency. Overall, knockdown of Meg3 alleviated formation of neovascularization in alkali-burned corneas and reduced VEGF-induced angiogenesis by inhibiting SDF-1/CXCR4 and Smad2/3 signaling in vitro.
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http://dx.doi.org/10.1016/j.exer.2022.109166DOI Listing
July 2022

Mitochondrial dysfunction in follicles is associated with broodiness in Zhedong white goose.

Anim Reprod Sci 2022 Aug 5;243:107032. Epub 2022 Jul 5.

Zhejiang Agriculture and Forestry University, Hangzhou 311300, China. Electronic address:

Zhedong white goose (Anser cygnoides) is one of the most important domesticated avian species and of high economic value in China. It has strong broody behavior, which leads to shortened egg-laying cycle, thus negatively impacting goose farming. In our previous studies, a link was established between reactive oxygen species (ROS) originated from mitochondrial activity and broodiness in geese. However, the role of mitochondria in the regulation of broodiness in geese remained unexplored. Herein, the transition from large white follicles to small yellow follicles was restrained in geese at the broody stage. Moreover, the frequency of abnormal mitochondria was increased in granulosa cells of broody follicles. Furthermore, mitochondrial dysfunction in granulosa cells of geese follicles is possibly regulated by mitochondria-related proteins including autophagy- and apoptosis-related proteins. Our results suggest that increased dysfunctional mitochondria in granulosa cells in the follicles of Zhedong white geese are involved in the regulation of broodiness. The present study contributes to expand the knowledge on goose broodiness and provides an important reference for the control of broodiness in avian species.
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http://dx.doi.org/10.1016/j.anireprosci.2022.107032DOI Listing
August 2022

Surface morphology properties and antifouling activity of BiWO/boron-grafted polyurethane composite coatings realized via multiple synergy.

J Colloid Interface Sci 2022 Jul 4;626:815-823. Epub 2022 Jul 4.

Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, China. Electronic address:

An inorganic-organic composite coating is an effective way to solve the issue of marine organism attachment and realize multi-element synergistic antifouling. Herein, BiWO/boron-grafted polyurethane composite coatings (BWOB) composed of BiWO with three morphologies (nanosheet, flower and microsphere) and boron-grafted polyurethane (ITB) were successfully synthesized to achieve high-efficiency antifouling. BiWO nanoparticles were evenly distributed on the surface and inside the ITB to form micro/nanostructures. In the composite coatings doped with flower-shaped BiWO, BWOB-5 showed excellent antibacterial and antidiatom adhesion properties, achieving 95.43% and 98.38% against Escherichia coli and Staphylococcus aureus, respectively, and 98.62% against Nitzschia closterium. In addition, the micro/nanostructure on the surface, the stable production of hydroxyl radicals (·OH) and superoxide radicals (·O) during photocatalysis, and the antifouling functional groups of the resin matrix in the BWOB composite coatings were all conducive to photocatalytic antifouling activity. More importantly, BWOB coatings exhibited excellent environmentally friendly properties. Therefore, BWOB coatings are expected to have potential application value in the field of photocatalytic sterilization and antifouling.
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http://dx.doi.org/10.1016/j.jcis.2022.06.180DOI Listing
July 2022

Efficacy of Phase II Remote Home Rehabilitation in Patients with Acute Myocardial Infarction after Percutaneous Coronary Intervention.

Contrast Media Mol Imaging 2022 13;2022:4634769. Epub 2022 Jun 13.

Department of Cardiology, Cangzhou Teaching Hospital of Hebei Medical University, Cangzhou Central Hospital, Cangzhou, Hebei, China.

Objective: To assess the efficacy of home-based cardiac rehabilitation and traditional outpatient rehabilitation in stage II after percutaneous coronary intervention (PCI) in patients with acute myocardial infarction (AMI).

Methods: From September 2019 to March 2020, 80 AMI patients in Cangzhou Central Hospital were randomly assigned to one of the two groups: the control group or the observation group, 40 cases in each group. The control group received old-fashioned outpatient rehabilitation treatment, and the study group received long-distance family rehabilitation nursing intervention. The blood pressure, examination results, compliance, satisfaction evaluation, incidence of cardiac events, heart rate, quality of life score, and 6-minute walking test were compared between the two groups.

Results: There were no deaths in both groups. There were significant differences in heart failure, unstable angina pectoris, unplanned readmission rate, walking compliance, and 6-minute walking test at 6 months after discharge ( < 0.05). There were substantial variances in left ventricular discharge portion, low-density lipoprotein, medication compliance, satisfaction, and quality of life ( < 0.05); there was substantial inconsistency in the 6-minute walking test concerning the two groups afterwards discharge for 3 months ( < 0.05).

Conclusion: Home rehabilitation is a new home cardiac rehabilitation model with high efficiency, convenience, and whole process monitoring and barrier-free follow-up management. It can effectively improve the cardiac function, workout patience and worth of life expectancy of victims with AMI, improve their self-management awareness and rehabilitation compliance, reduce the risk of cardiac events, and have a positive impact on the prognosis and rehabilitation of patients with AMI.
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http://dx.doi.org/10.1155/2022/4634769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208999PMC
July 2022

Roles of EP Receptors in the Regulation of Fluid Balance and Blood Pressure.

Front Endocrinol (Lausanne) 2022 23;13:875425. Epub 2022 Jun 23.

Jiangsu Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, China.

Prostaglandin E2 (PGE2) is an important prostanoid expressing throughout the kidney and cardiovascular system. Despite the diverse effects on fluid metabolism and blood pressure, PGE2 is implicated in sustaining volume and hemodynamics homeostasis. PGE2 works through four distinct E-prostanoid (EP) receptors which are G protein-coupled receptors. To date, pharmacological specific antagonists and agonists of all four subtypes of EP receptors and genetic targeting knockout mice for each subtype have helped in uncoupling the diverse functions of PGE2 and discriminating the respective characteristics of each receptor. In this review, we summarized the functions of individual EP receptor subtypes in the renal and blood vessels and the molecular mechanism of PGE2-induced fluid metabolism and blood pressure homeostasis.
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http://dx.doi.org/10.3389/fendo.2022.875425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262144PMC
July 2022

Research on the Mechanism and Prevention of Hypertension Caused by Apatinib Through the RhoA/ROCK Signaling Pathway in a Mouse Model of Gastric Cancer.

Front Cardiovasc Med 2022 23;9:873829. Epub 2022 Jun 23.

Department of Hypertension Center, Lanzhou University Second Hospital, Lanzhou, China.

Hypertension is one of the main adverse effects of antiangiogenic tumor drugs and thus limits their application. The mechanism of hypertension caused by tyrosine kinase inhibitors (TKIs) targeting vascular endothelial growth factors is mainly related to inhibition of the nitric oxide (NO) pathway and activation of the endothelin pathway, as well as vascular rarefaction and increased salt sensitivity; consequently, prevention and treatment differ for this type of hypertension compared with primary hypertension. Apatinib is a highly selective TKI approved in China for the treatment of advanced or metastatic gastric cancer. The RhoA/ROCK pathway is involved in the pathogenesis of hypertension and mediates smooth muscle contraction, eNOS inhibition, endothelial dysfunction and vascular remodeling. In this study, experiments were performed to explore whether the RhoA/ROCK signaling pathway is part of a possible mechanism of apatinib in the treatment of gastric cancer-induced hypertension and the impairment of vascular remodeling and left ventricular function. Y27632, a selective small inhibitor of both ROCK1 and ROCK2, was combined with apatinib, and its efficacy was evaluated, wherein it can reduce hypertension induced by apatinib treatment in gastric cancer mice and weaken the activation of the RhoA/ROCK pathway by apatinib and a high-salt diet (HSD). Furthermore, Y-27632 improved aortic remodeling, fibrosis, endothelial dysfunction, superior mesenteric artery endothelial injury, left ventricular dysfunction and cardiac fibrosis in mice by weakening the activation of the RhoA/ROCK pathway. The expression of RhoA/ROCK pathway-related proteins and relative mRNA levels in mice after apatinib intervention were analyzed by various methods, and blood pressure and cardiac function indexes were compared. Endothelial and cardiac function and collagen levels in the aorta were also measured to assess vascular and cardiac fibrosis and to provide a basis for the prevention and treatment of this type of hypertension.
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http://dx.doi.org/10.3389/fcvm.2022.873829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9262125PMC
June 2022

CHRNA1 induces sarcopenia through neuromuscular synaptic elimination.

Exp Gerontol 2022 Sep 6;166:111891. Epub 2022 Jul 6.

Department of Geriatrics, the First Affiliated Hospital of Chongqing Medical University, Friendship Road 1, Yuan Jiagang, 400016 Chongqing, China. Electronic address:

Sarcopenia seriously affects the quality of life of the elderly, but its molecular mechanism is still unclear. Degeneration in muscle innervation is related to age-related movement disorders and muscle atrophy. The expression of CHRNA1 is increased in the skeletal muscle of the elderly, and in aging rodents. Therefore, we investigated whether CHRNA1 induces the occurrence and development of sarcopenia. Compared with the control group, local injection of AAV9-CHRNA1 into the hindlimb muscles decreased the percentage of muscle innervation. At the same time, the skeletal muscle mass decreased, as manifested by a decrease in the gastrocnemius mass index and the cross-sectional area of the muscle fibers. The function of skeletal muscle also decreased, which was manifested by decreases of compound muscle action potential and muscle contractility. Therefore, we concluded that upregulation of CHRNA1 can induce and aggravate sarcopenia.
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http://dx.doi.org/10.1016/j.exger.2022.111891DOI Listing
September 2022
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