Publications by authors named "Jing Xue"

527 Publications

Activated PI3K/AKT/mTOR signal and suppressed autophagy participate in the protection offered by licochalcone A against Aβ25-35-induced injury in SH-SY5Y cells.

World Neurosurg 2021 Oct 15. Epub 2021 Oct 15.

Xi'an Dongao Biosciences Co., Ltd., Xi'an 710000, China;. Electronic address:

In this study, we assessed the effect of Licochalcone A (LicA) on amyloid beta-peptide 25-35 (Aβ25-35)-induced nerve injury and the potential molecular mechanisms involved. The cell viability of SH-SY5Y cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay after treatment with Aβ25-35 and/or LicA, following which, apoptosis was detected by flow cytometry (after PI & Annexin V-FITC or JC-1 staining) and Hoechst staining. Then, reactive oxygen species (ROS), glutathione (GSH), and superoxide dismutase (SOD) were measured with flow cytometry and spectrophotometry. The ultra-structure of mitochondria was examined by transmission electron microscopy (TEM), and the biomarker proteins of autophagy, apoptosis, and PI3K/AKT/mTOR signaling pathway were measured with western blotting. LicA improved cell viability and decreased lactate dehydrogenase (LDH) leakage remarkably in Aβ25-35-induced injury in SH-SY5Y cells. After treatment with LicA, the levels of ROS, GSH, and SOD in cells were all significantly decreased, which indicated that LicA has antioxidative effect on Aβ25-35-induced oxidic injury. LicA could also significantly reduce Aβ25-35-induced autophagy in SH-SY5Y cells. In the cells injured by Aβ25-35, LicA prevented the transformation from light chain 3-I to light chain 3-II and reduced the levels of GRP78, GRP94, CHOP, and Bax, but increased the levels of anti-apoptotic protein and phosphorylation of PI3K, AKT, and mTOR. These effects of LicA were restored or suppressed by mTOR inhibitor rapamycin or PI3K inhibitor LY294002. Thus, LicA protects SH-SY5Y cells against Aβ25-35-induced injury, wherein, suppressed autophagy and activated PI3K/AKT/mTOR signaling pathway are involved, and mTOR-dependent autophagy at least plays some roles.
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http://dx.doi.org/10.1016/j.wneu.2021.10.098DOI Listing
October 2021

Soluble programmed death molecule 1 (sPD-1) as a predictor of interstitial lung disease in rheumatoid arthritis.

BMC Immunol 2021 Oct 15;22(1):69. Epub 2021 Oct 15.

Department of Rheumatology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.

Background: Previous studies have indicated that the programmed death molecule 1 (PD-1) signaling pathway may play a key role in rheumatoid arthritis (RA). However, the pathogenesis of rheumatoid arthritis-related interstitial lung disease (RA-ILD) is not clear. We examined the serum levels of soluble PD-1 in patients with RA and its relationship with RA-ILD.

Methods: Blood samples were obtained from 87 patients with RA (58 with ILD and 29 without ILD) and 45 healthy controls. Serum sPD-1 was measured by Enzyme-Linked Immunosorbent Assay. The pulmonary interstitial disease score was completed by a pulmonary physician and a radiologist through chest high-resolution computed tomography. Patients with RA-ILD were tested for lung function [e.g., forced vital capacity (FVC%), diffusing capacity of lungs for carbon monoxide (DLCO%)]. Associations between ILD and various markers, including sPD-1 and confounding factors, were investigated by logistic regression analysis. Diagnostic values of sPD-1 for the presence of ILD were investigated using receiver operating characteristic curve analysis.

Results: Serum sPD-1 levels were higher in RA patients with ILD than in RA patients without ILD and healthy controls (185.1 ± 109.0 pg/ml vs. 119.1 ± 77.5 pg/ml vs. 52.1 ± 21.7 pg/ml, P < 0.05). Serum sPD-1 levels were positively correlated with RF titer (P = 0.02, r = 0.249), anti-cyclic citrullinated peptide antibody status (P = 0.02, r = 0.243), and serum IgG levels (P < 0.001, r = 0.368), negatively associated with FVC% (P = 0.02, r = - 0.344), forced expiratory volume (FEV1%) (P  = 0.01, r = - 0.354), total lung capacity (TLC%) (P = 0.046, r = - 0.302), and was independently associated with the presence of ILD in RA patients by multivariate logistic regression analysis. The sensitivity and specificity of sPD-1 levels for the detection of ILD in RA patients were 58.6% and 75.9%, respectively. The area under the curve was 0.689.

Conclusion: Serum sPD-1 levels were increased in RA patients with ILD. Increased sPD-1 may be a valuable biomarker to predict the presence of ILD in patients with RA.
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http://dx.doi.org/10.1186/s12865-021-00460-6DOI Listing
October 2021

Minimally invasive versus open transforaminal lumbar interbody fusion for single segmental lumbar disc herniation: A meta-analysis.

J Back Musculoskelet Rehabil 2021 Sep 24. Epub 2021 Sep 24.

Background: Numerous studies on the comparison of minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) and open-transforaminal lumbar interbody fusion (O-TLIF) for the treatment of lumbar disc herniation (LDH) have been published, but there is no clear conclusion.

Objective: The aim of this study was to evaluate the efficacy of MIS-TLIF compared with O-TLIF in the treatment of LDH in the Chinese population by meta-analysis.

Methods: Studies on the treatment of LDH by MIS-TLIF versus O-TLIF were searched in Pubmed, Web of Science, Medline, Embase, CNKI, VIP and China Wanfang databases from the establishment of the databases to January 2020. The meta-analysis was used to analyze the pooled operation time, intraoperative blood loss, postoperative drainage, postoperative ground movement time, Waist and leg Visual Analogue Scale (VAS) score, Oswestry Disability Index (ODI) score and Japanese orthopaedic association (JOA) score. Mean difference (MD) and standard mean difference (SMD) were used as the effect size.

Results: Eleven studies with 1132 patients were included. The results showed that MIS-TLIF compared with O-TLIF, MD =-133.82 (95% CI: -167.10 ∼-100.53, P< 0.05) in intraoperative blood loss, MD =-114.43 (95% CI: -141.12 ∼-87.84, P< 0.05) in postoperative drainage, MD =-3.30 (95% CI: -4.31 ∼-2.28, P< 0.05) in postoperative ground movement time, SMD =-1.44 (95% CI: -2.63 ∼-0.34, P< 0.05) in postoperative low back pain VAS score, SMD = 0.41 (95% CI: 0.15 ∼ 0.66, P< 0.05) in postoperative JOA score, MD = 4.12 (95% CI: -11.64 ∼ 19.87, P> 0.05) in the average operation time, SMD =-0.00 (95% CI: -0.36 ∼ 0.36, P> 0.05) in leg pain VAS score, and SMD =-0.59 (95% CI: -1.22 ∼ 0.03, P> 0.05) in ODI score.

Conclusion: MIS-TLIF was superior to O-TLIF in the treatment of LDH, especially in the intraoperative blood loss, postoperative drainage, postoperative ground movement time and low back pain in the Chinese population.
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http://dx.doi.org/10.3233/BMR-210004DOI Listing
September 2021

Construction of CF-Functionalized Fully Substituted Benzonitriles through Rauhut-Currier Reaction Initiated [3 + 3] Benzannulation.

J Org Chem 2021 Oct 1. Epub 2021 Oct 1.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, People's Republic of China.

Though numerous cyanation reactions have been developed for the synthesis of benzonitriles, the construction of valuable fully substituted benzonitriles is still a challenging task. Herein, we reported a tertiary amine-catalyzed [3 + 3]-benzannulation for the green synthesis of CF-functionalized fully substituted benzonitriles. This strategy features exclusive chemoselectivity, high atom-economy, and good step-economy with environment-friendly reagents and mild conditions. Unique triphenyl-substituted dicyanobenzoate products could be rapidly constructed using this method. The practicality and reliability of this reaction were proved by the successful scale-up synthesis. A mechanistic study indicates that the [3 + 3]-benzannulation was initiated by an intermolecular Rauhut-Currier reaction.
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http://dx.doi.org/10.1021/acs.joc.1c01631DOI Listing
October 2021

Screening and identification of differentially expressed microRNAs in diffuse large B-cell lymphoma based on microRNA microarray.

Oncol Lett 2021 Nov 27;22(5):753. Epub 2021 Aug 27.

Department of Pathology, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.

Diffuse large B-cell lymphoma (DLBCL) is the most common type of B-cell non-Hodgkin lymphoma in adults and the pathogenesis of DLBCL is multifactorial and complex. Understanding the molecular mechanisms involved in DLBCL is important to identify new therapeutic targets. The present study aimed to screen and identify differentially expressed microRNAs (miRNAs/miRs) between diffuse large B-cell lymphoma (DLBCL) and control [lymph node reactive hyperplasia (LRH)] groups, and to investigate whether miRNAs associated with DLBCL could serve as potential therapeutic targets. In total, 5 DLBCL experimental samples and 5 control samples were obtained from fresh patient tissues. Firstly, the fresh samples were analyzed using miRNA microarray to identify differentially expressed miRNAs. Next, three databases (TargetScan, microRNA.org and PITA) were used to predict by intersection the potential target genes of the 204 differential miRNAs identified, and a Venn diagram of the results was performed. Subsequently, the target genes of differential miRNAs were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. Finally, to validate the miRNA microarray data, reverse transcription-quantitative PCR (RT-qPCR) was performed for 8 differentially expressed miRNAs (miR-193a-3p, miR-19a-3p, miR-19b-3p, miR-370-3p, miR-1275, miR-490-5p, miR-630 and miR-665) using DLBCL and LRH fresh samples. In total, 204 miRNAs exhibited differential expression, including 105 downregulated and 54 upregulated miRNAs. The cut-off criteria were set as P≤0.05 and fold-change ≥2. A total of 7,522 potential target genes for the 204 miRNAs were predicted. Potential target genes were enriched in the following pathways: 'Cancer', 'MAPK signaling pathway', 'regulation of actin cytoskeleton', 'focal adhesion', 'endocytosis', 'Wnt signaling pathway', 'axon guidance', 'calcium signaling pathway' and 'PI3K/AKT signaling pathway'. A total of 8 miRNAs were validated by RT-qPCR, and 4 miRNAs (miR-19b-3p, miR-193a-3p, miR-370-3p and miR-490-5p) exhibited low expression levels in DLBCL (P<0.05), while miR-630 was highly expressed in DLBCL (P<0.05). Overall, the present study screened 204 differentially expressed miRNAs and analyzed the expression levels of 8 differentially expressed miRNAs in DLBCL. These differentially expressed miRNAs may serve as therapeutic targets for improvement of therapeutic efficacy in DLBCL in the future.
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http://dx.doi.org/10.3892/ol.2021.13014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436336PMC
November 2021

A PLA2R-IgG4 Antibody-Based Predictive Model for Assessing Risk Stratification of Idiopathic Membranous Nephropathy.

J Healthc Eng 2021 31;2021:1521013. Epub 2021 Aug 31.

Department of Nephrology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi 214023, China.

Background: Known as an autoimmune glomerular disease, idiopathic membranous nephropathy (IMN) is considered to be associated with phospholipase A2 receptor (PLA2R) in terms of the main pathogenesis. The quantitative detection of serum PLA2R-IgG and PLA2R-IgG4 antibodies by time-resolved fluoroimmunoassay (TRFIA) was determined, and the value of them, both in the clinical prediction of risk stratification in IMN, was observed in this study.

Methods: 95 patients with IMN proved by renal biopsy were enrolled, who had tested positive for serum PLA2R antibodies by ELISA, and the quantitative detection of serum PLA2R-IgG and PLA2R-IgG4 antibodies was achieved by TRFIA. All the patients were divided into low-, medium-, and high-risk groups, respectively, which were set as dependent variables, according to proteinuria and renal function. Random forest (RF) was used to estimate the value of serum PLA2R-IgG and PLA2R-IgG4 in predicting the risk stratification of progression in IMN.

Results: Out-of-bag estimates of variable importance in RF were employed to evaluate the impact of each input variable on the final classification accuracy. The variable of albumin, PLA2R-IgG, and PLA2R-IgG4 had high values (>0.3) of 0.3156, 0.3981, and 0.7682, respectively, which meant that these three were more important for the risk stratification of progression in IMN. In order to further assess the contribution of PLA2R-IgG and PLA2R-IgG4 to the model, we built four different models and found that PLA2R-IgG4 played an important role in improving the predictive ability of the model.

Conclusions: In this study, we established a random forest model to evaluate the value of serum PLA2R-IgG4 antibodies in predicting risk stratification of IMN. Compared with PLA2R-IgG, PLA2R-IgG4 is a more efficient biomarker in predicting the risk of progression in IMN.
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http://dx.doi.org/10.1155/2021/1521013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424241PMC
August 2021

Rheological and Mechanical Properties of Resin-Based Materials Applied in Dental Restorations.

Polymers (Basel) 2021 Sep 1;13(17). Epub 2021 Sep 1.

School of Chemistry and Chemical Engineering, Shandong University of Technology, 266 Xincun Rd., Zibo 255000, China.

Resin-based materials have been prevalent for dental restorations over the past few decades and have been widely used for a variety of direct and indirect procedures. Typically, resin-based dental materials are required to be flowable or moldable before setting and can provide adequate mechanical strength after setting. The setting method may include, but is not limited to, light-curing, self-curing or heating. In this review, based on different indications of resin-based dental materials (e.g., dental filling composite, dental bonding agent, resin luting cement), their rheological and mechanical properties were reviewed. Viscous and flexible properties were focused on for materials before setting, while elastic properties and mechanical strength were focused on for materials after setting. At the same time, the factors that may affect their rheological and mechanical properties were discussed. It is anticipated that the insightful information and prospections of this study will be useful to the future development and fabrication of resin-based dental restorative materials.
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http://dx.doi.org/10.3390/polym13172975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433687PMC
September 2021

Ccq1-Raf2 interaction mediates CLRC recruitment to establish heterochromatin at telomeres.

Life Sci Alliance 2021 11 7;4(11). Epub 2021 Sep 7.

State Key Laboratory of Oncogenes and Related Genes, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

Telomeres, highly ordered DNA-protein complexes at eukaryotic linear chromosome ends, are specialized heterochromatin loci conserved among eukaryotes. In , the shelterin complex is important for subtelomeric heterochromatin establishment. Despite shelterin has been demonstrated to mediate the recruitment of the Snf2/histone deacetylase-containing repressor complex (SHREC) and the Clr4 methyltransferase complex (CLRC) to telomeres, the mechanism involved in telomeric heterochromatin assembly remains elusive due to the multiple functions of the shelterin complex. Here, we found that CLRC plays a dominant role in heterochromatin establishment at telomeres. In addition, we identified a series of amino acids in the shelterin subunit Ccq1 that are important for the specific interaction between Ccq1 and the CLRC subunit Raf2. Finally, we demonstrated that the Ccq1-Raf2 interaction is essential for the recruitment of CLRC to telomeres, that contributes to histone H3 lysine 9 methylation, nucleosome stability and the shelterin-chromatin association, promoting a positive feedback mechanism for the nucleation and spreading of heterochromatin at subtelomeres. Together, our findings provide a mechanistic understanding of subtelomeric heterochromatin assembly by shelterin-dependent CLRC recruitment to chromosomal ends.
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http://dx.doi.org/10.26508/lsa.202101106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424379PMC
November 2021

SARS-CoV-2 crosses the blood-brain barrier accompanied with basement membrane disruption without tight junctions alteration.

Signal Transduct Target Ther 2021 09 6;6(1):337. Epub 2021 Sep 6.

NHC Key Laboratory of Human Disease Comparative Medicine, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China.

SARS-CoV-2 has been reported to show a capacity for invading the brains of humans and model animals. However, it remains unclear whether and how SARS-CoV-2 crosses the blood-brain barrier (BBB). Herein, SARS-CoV-2 RNA was occasionally detected in the vascular wall and perivascular space, as well as in brain microvascular endothelial cells (BMECs) in the infected K18-hACE2 transgenic mice. Moreover, the permeability of the infected vessel was increased. Furthermore, disintegrity of BBB was discovered in the infected hamsters by administration of Evans blue. Interestingly, the expression of claudin5, ZO-1, occludin and the ultrastructure of tight junctions (TJs) showed unchanged, whereas, the basement membrane was disrupted in the infected animals. Using an in vitro BBB model that comprises primary BMECs with astrocytes, SARS-CoV-2 was found to infect and cross through the BMECs. Consistent with in vivo experiments, the expression of MMP9 was increased and collagen IV was decreased while the markers for TJs were not altered in the SARS-CoV-2-infected BMECs. Besides, inflammatory responses including vasculitis, glial activation, and upregulated inflammatory factors occurred after SARS-CoV-2 infection. Overall, our results provide evidence supporting that SARS-CoV-2 can cross the BBB in a transcellular pathway accompanied with basement membrane disrupted without obvious alteration of TJs.
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http://dx.doi.org/10.1038/s41392-021-00719-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419672PMC
September 2021

Adenoviral Transduction of Alleviates Silica-Induced Silicosis Development in Lungs of Mice.

Hum Gene Ther 2021 Sep 28. Epub 2021 Sep 28.

Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources of Western China, College of Life Science, Ningxia University, Yinchuan, China.

Silicosis is an occupational disease caused by inhalation of silica dust, which is hallmarked by progressive pulmonary fibrosis associated with poor prognosis. Wnt/β-catenin signaling is implicated in the development of fibrosis and is a therapeutic target for fibrotic diseases. Previous clinical studies of patients with pneumoconiosis, including silicosis, revealed an increased concentration of circulating WNT3A and DKK1 proteins and inflammatory cells in bronchoalveolar lavage compared with healthy subjects. The present study evaluated the effects of adenovirus-mediated transduction of (), a Wnt/β-catenin signaling inhibitor, on the development of pulmonary silicosis in mice. Consistent with previous human clinical studies, our experimental studies in mice demonstrated an aberrant Wnt/β-catenin signaling activity coinciding with increased Wnt3a and Dkk1 proteins and inflammation in lungs of silica-induced silicosis mice compared with controls. Intratracheal delivery of adenovirus expressing murine Dkk1 (AdDkk1) inhibited Wnt/β-catenin activity in mouse lungs. The adenovirus-mediated gene transduction demonstrated the potential to prevent silicosis development and ameliorate silica-induced lung fibrogenesis in mice, accompanied by the reduced expression of epithelia--mesenchymal transition markers and deposition of extracellular matrix proteins compared with mice treated with "null" adenoviral vector. Mechanistically, AdDkk1 is able to attenuate the lung silicosis by inhibiting a silica-induced spike in TGF-β/Smad signaling. In addition, the forced expression of Dkk1 suppressed silica-induced epithelial cell proliferation in polarized human bronchial epithelial cells. This study provides insight into the underlying role of Wnt/β-catenin signaling in promoting the pathogenesis of silicosis and is proof-of-concept that targeting Wnt/β-catenin signaling by gene transduction may be an alternative approach in the prevention and treatment of silicosis lung disease.
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http://dx.doi.org/10.1089/hum.2021.008DOI Listing
September 2021

Characterization of Endophytic Fungi, sp., from and Analysis of Its Antifungal and Plant Growth-Promoting Effects.

Biomed Res Int 2021 3;2021:9930210. Epub 2021 Aug 3.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

The present study was aimed at isolating endophytic fungi from the Asian culinary and medicinal plant and analyzing its antifungal and plant growth-promoting effects. In this study, the fungal endophyte sp. Ld-03 was isolated from the bulbs of and identified through morphological and molecular analysis. The molecular and morphological analysis confirmed the endophytic fungal strain as sp. Ld-03. Antifungal effects of Ld-03 were observed against , , , and . The highest growth inhibition, i.e., 78.39 ± 4.21%, was observed for followed by 56.68 ± 4.38%, 43.62 ± 3.81%, and 20.12 ± 2.45% for , , and , respectively. Analysis of the ethyl acetate fraction through UHPLC-LTQ-IT-MS/MS revealed putative secondary metabolites which included xanthurenic acid, valyl aspartic acid, gancidin W, peptides, and cyclic dipeptides such as valylarginine, cyclo-[L-(4-hydroxy-Pro)-L-leu], cyclo(Pro-Phe), and (3S,6S)-3-benzyl-6-(4-hydroxybenzyl)piperazine-2,5-dione. Other metabolites included (S)-3-(4-hydroxyphenyl)-2-((S)-pyrrolidine-2-carboxamido)propanoic acid, dibutyl phthalate (DBP), 9-octadecenamide, D-erythro-C18-Sphingosine, N-palmitoyl sphinganine, and hydroxypalmitoyl sphinganine. The strain Ld-03 showed indole acetic acid (IAA) production with or without the application of exogenous tryptophan. The IAA ranged from 53.12 ± 3.20 g ml to 167.71 ± 7.12 g ml under different tryptophan concentrations. The strain was able to produce siderophore, and its production was significantly decreased with increasing Fe(III) citrate concentrations in the medium. The endophytic fungal strain also showed production of organic acids and phosphate solubilization activity. Plant growth-promoting effects of the strain were evaluated on seedling growth of . Application of 40% culture dilution resulted in a significant increase in root and shoot length, i.e., 24.03 ± 2.71 mm and 37.27 ± 1.86 mm, respectively, compared to nontreated control plants. The fungal endophyte Ld-03 demonstrated the potential of conferring disease resistance and plant growth promotion. Therefore, we conclude that the isolated sp. Ld-03 should be further investigated before utilization as a biocontrol agent and plant growth stimulator.
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http://dx.doi.org/10.1155/2021/9930210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358427PMC
September 2021

Comparing the association of cardiovascular nursing care with blood pressure and length of stay of in-patients with coronary artery disease in Wuhan, China.

Afr Health Sci 2020 Dec;20(4):1716-1724

College of Arts and Science of Jianghan University.

Background: Coronary artery disease is a leading cause of morbidity and mortality worldwide. Comorbidity-like hypertension has been among the major risks of coronary artery disease. Recent evidence identified multiple benefits of cardiovascular nursing care to coronary patients. However, little has been appraised on benefits regarding patients' blood pressure control and length of hospitalisation.

Objective: To compare the association of cardiovascular nursing care delivered to coronary artery patients with patients' blood pressure and length of stay.

Methods: Records based retrospective design was applied at a large teaching hospital in Wuhan, China. SPSS 21 version was used for data entry and analysis with univariate and multivariate logistic regression models for comparing study variables.

Results: Of 300 patients, 224 (74.7%) were known to be hypertensive and admitted with subnormal blood pressure. Cardiovascular nursing care like "assess to grade pain severity on 1-10 scale" and "counsel patient to cope with stress" were six and three times more likely to contribute improved patients' blood pressure (AOR=5.8; 95%CI: 2.8-12.2, p=0.001) and (AOR=3.1; 95%CI: 1.2-7.8, p=0.015) respectively. No significant difference with length of stay (p>0.05).

Conclusion: There is a possibility of coronary artery patients to recover with normal blood pressure following reception of evidence-based cardiovascular nursing care.
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http://dx.doi.org/10.4314/ahs.v20i4.23DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351856PMC
December 2020

A Domain Adaptation Sparse Representation Classifier for Cross-Domain Electroencephalogram-Based Emotion Classification.

Front Psychol 2021 29;12:721266. Epub 2021 Jul 29.

Department of Clinical Psychology, The Third Affiliated Hospital of Soochow University, Changzhou, China.

The brain-computer interface (BCI) interprets the physiological information of the human brain in the process of consciousness activity. It builds a direct information transmission channel between the brain and the outside world. As the most common non-invasive BCI modality, electroencephalogram (EEG) plays an important role in the emotion recognition of BCI; however, due to the individual variability and non-stationary of EEG signals, the construction of EEG-based emotion classifiers for different subjects, different sessions, and different devices is an important research direction. Domain adaptation utilizes data or knowledge from more than one domain and focuses on transferring knowledge from the source domain (SD) to the target domain (TD), in which the EEG data may be collected from different subjects, sessions, or devices. In this study, a new domain adaptation sparse representation classifier (DASRC) is proposed to address the cross-domain EEG-based emotion classification. To reduce the differences in domain distribution, the local information preserved criterion is exploited to project the samples from SD and TD into a shared subspace. A common domain-invariant dictionary is learned in the projection subspace so that an inherent connection can be built between SD and TD. In addition, both principal component analysis (PCA) and Fisher criteria are exploited to promote the recognition ability of the learned dictionary. Besides, an optimization method is proposed to alternatively update the subspace and dictionary learning. The comparison of CSFDDL shows the feasibility and competitive performance for cross-subject and cross-dataset EEG-based emotion classification problems.
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http://dx.doi.org/10.3389/fpsyg.2021.721266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358659PMC
July 2021

TMEM120A is a coenzyme A-binding membrane protein with structural similarities to ELOVL fatty acid elongase.

Elife 2021 08 10;10. Epub 2021 Aug 10.

Department of Physiology, University of Texas Southwestern Medical Center, Dallas, United States.

TMEM120A, also named as TACAN, is a novel membrane protein highly conserved in vertebrates and was recently proposed to be a mechanosensitive channel involved in sensing mechanical pain. Here we present the single-particle cryogenic electron microscopy (cryo-EM) structure of human TMEM120A, which forms a tightly packed dimer with extensive interactions mediated by the N-terminal coiled coil domain (CCD), the C-terminal transmembrane domain (TMD), and the re-entrant loop between the two domains. The TMD of each TMEM120A subunit contains six transmembrane helices (TMs) and has no clear structural feature of a channel protein. Instead, the six TMs form an α-barrel with a deep pocket where a coenzyme A (CoA) molecule is bound. Intriguingly, some structural features of TMEM120A resemble those of elongase for very long-chain fatty acids (ELOVL) despite the low sequence homology between them, pointing to the possibility that TMEM120A may function as an enzyme for fatty acid metabolism, rather than a mechanosensitive channel.
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http://dx.doi.org/10.7554/eLife.71220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376247PMC
August 2021

Stage-Dependent Within-Individual Comparison Reveals SIV-Specific Activation/Exhaustion Shift in Rhesus Macaques.

Front Microbiol 2021 19;12:704449. Epub 2021 Jul 19.

Key Laboratory of Human Disease Comparative Medicine, Chinese Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China.

It is challenging to trace the complicated individual-based variations of HIV-specific immunocompetence shift during the successful antiretroviral therapy (ART) era. Using eight rhesus monkeys simulating a longitudinal stage-dependent cohort (baseline-SIV acute infection-SIV suppression by ART-ART withdrawal), baseline immunocompetence monitoring for 28 days (SIV-negative stage, SN) was compared with host immunocompetence undergoing 90-day ART treatment (SIV-suppressed stage, SS) to reveal the SIV-specific immunity shift aroused by undetectable individual viral replication. During acute SIV infection for 98 days (SIV-emerged stage, SE), immune activation was compared with re-immune activation post ART for 49-day follow-up (SIV-rebounded stage, SR) to reveal the SIV-specific immune activation variation aroused by detectable individual viral replication. Individual immunocompetence was measured by co-expression of CD4, CD8, CD38, HLA-DR, CCR7, CD45RA, and PD-1 on T cells and a cytokine panel. Compared with SN, mild immune activation/exhaustion was characterized by increased CD38 HLA-DR CD4/CD8 T-cell subsets and PD-1 memory CD4/CD8 T-cell subsets with three elevated cytokines (MIP-1β, IL-8, and IL-10) significantly emerged in SS. Compared with SE, SR produced more exhaustion characterized by increased PD-1 CD4 T cells and decreased PD-1 CD4 T cells with four elevated pro-inflammatory cytokines (IFN-γ, IL-1β, IL-6, and TNF-α). By such individualized stage-dependent comparison, the sustainable immune activation was found from activation/exhaustion shifted into exhaustion during the longitudinal viral persistence. Further, validated SIV accelerates host immunosenescence continuously independent of viral replication.
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http://dx.doi.org/10.3389/fmicb.2021.704449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326587PMC
July 2021

Bioorthogonal Chemical Signature Enabling Amplified Visualization of Cellular Oxidative Thymines.

Anal Chem 2021 08 22;93(30):10495-10501. Epub 2021 Jul 22.

Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xianning West Road, Xi'an 710049, Shaanxi, P. R. China.

Cellular oxidative thymines, 5-hydroxymethyluracil (5hmU) and 5-formyluracil (5fU), are found in the genomes of a diverse range of organisms, the distribution of which profoundly influence biological processes and living systems. However, the distribution of cellular oxidative thymines has not been explored because of lacking both specific bioorthogonal labeling and sensitivity methods for single-cell analysis. Herein, we report a bioorthogonal chemical signature enabling amplified visualization of cellular oxidative thymines in single cells. The synthesized ATP-γ-alkyne, an ATP analogue with bioorthogonal tag modified on γ-phosphate can be specifically linked to cellular 5hmU by chemoenzymatic labeling. DNA with 5-alkynephosphomethyluracil were then clicked with azide (N)-modified 5hmU-primer. Identification of 5fU is based on selective reduction from 5fU to 5hmU, subsequent chemoenzymatic labeling of the newly generated 5hmU, and cross-linking with N-modified 5fU-primer via click chemistry. Then, all of the 5hmU and 5fU sites are encoded with respective circularized barcodes. These barcodes are simultaneously amplified for multiplexed single-molecule imaging. The above two kinds of barcodes can be simultaneously amplified for differentiated visualization of 5hmU and 5fU in single cells. We find these two kinds of cellular oxidative thymines are spatially organized in a cell-type-dependent style with cell-to-cell heterogeneity. We also investigate their multilevel subcellular information and explore their dynamic changes during cell cycles. Further, using DNA sequencing instead of fluorescence imaging, our proposed bioorthogonal chemical signature holds great potential to offer the sequence information of these oxidative thymines in cells and may provide a reliable chemical biology approach for studying the whole-genome oxidative thymines profiles and insights into their functional role and dynamics in biology.
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http://dx.doi.org/10.1021/acs.analchem.1c01285DOI Listing
August 2021

Quantitative Microbial Risk Assessment of and in Public Drinking Water in China.

Biomed Environ Sci 2021 Jun;34(6):493-498

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), Shanghai 200025, China;NHC Key Laboratory of Parasite and Vector Biology, Shanghai 200025, China;WHO Collaborating Centre for Tropical Diseases, Shanghai 200025, China;National Center for International Research on Tropical Diseases, Shanghai 200025, China;School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

We aimed to assess the risks of and infections associated with drinking water for local residents, based on a quantitative microbial risk assessment, in three densely populated regions of China. In total, 45 source water samples and 45 treated water samples were collected from June to December 2014. Five -positive samples and 5 -positive samples were found. The annual probability of infection for individuals in Jintan (6.27 × 10 -2.05 × 10 for and 7.18 × 10 -2.32 × 10 for ), Ezhou (6.27 × 10 -1.10 × 10 for and 3.65 × 10 -1.20 × 10 for ), and Binyang (3.79 × 10 -1.25 × 10 for ) exceeded the tolerable risk of infection of 10 set by the United States Environmental Protection Agency. Moreover, the corresponding disease burdens of cryptosporidiosis and giardiasis, due to direct drinking and residual water in these regions, exceeded the threshold of 10 disability-adjusted life years per person per year set by the World Health Organization. These results provide insights into strategies to improve the safety of drinking water.
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http://dx.doi.org/10.3967/bes2021.068DOI Listing
June 2021

Thymosin beta 4 alleviates non-alcoholic fatty liver by inhibiting ferroptosis via up-regulation of GPX4.

Eur J Pharmacol 2021 Oct 16;908:174351. Epub 2021 Jul 16.

Department of Blood Transfusion, The Affliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China. Electronic address:

Thymosin beta 4 (Tβ4) can improve the liver fibrosis and reduce inflammation, while the role of Tβ4 in non-alcoholic fatty liver disease (NAFLD) whether mediated by ferroptosis remains unclear. A rat model of NAFLD was established on a high-fat diet (HFD), and rats were assigned ferroptosis inducer erastin and inhibitor Ferrostatin 1 (Fer-1). Subsequently, histopathology of the liver and the expression of ferroptosis-related genes in rat liver were detected. The steatosis of LO2 cells was induced by palmitic acid (PA) to reproduce the results of the rat experiment. The small interfering RNA (siRNA) was used to interfere with GPX4 expression to explore the influence on Tβ4 function. Tβ4 improved the inflammation, biochemical and lipid metabolism indexes, increased the antioxidant level, and inhibited abnormal accumulation of intracellular reactive oxygen species in HFD-induced NAFLD rats. Also, Tβ4 improved PA-induced LO2 damage and inhibited apoptosis of PA-induced LO2 cells. Both in vivo and in vitro, Tβ4 regulated expression of genes associated with ferroptosis, and Fer-1 treatment exaggerated the above effects of Tβ4, while erastin attenuated the protective effect of Tβ4. Moreover, siRNA GPX4 attenuated the protective effect of Tβ4 on the rat liver and on the mitochondrial membrane integrity of LO2 cells. Interfered expression of GPX4 with siRNA also regulated the expression of Bcl-2, Bax, Caspase-3 and SOD1, which attenuated therapeutic effect of Tβ4 on rat liver and LO2 cells. This study revealed that Tβ4 protects hepatocytes by inhibiting the GPX4-mediated ferroptosis pathway, which provides a new strategy and target for the treatment of NAFLD.
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http://dx.doi.org/10.1016/j.ejphar.2021.174351DOI Listing
October 2021

Enhanced Catalysis of P-doped SnO for the V/V Redox Reaction in Vanadium Redox Flow Battery.

Front Chem 2021 24;9:688634. Epub 2021 Jun 24.

School of Chemical Engineering, North China University of Science and Technology, Tangshan, China.

In this work, nanosized P-doped SnO (SnO-P) was prepared by a sol-gel method as a catalyst for the V/V redox reaction in vanadium redox flow battery. Compared with SnO, the electrochemical performance of SnO-P is significantly improved. This is because P doping provides more active sites and shows greatly improved electrical conductivity, thereby increasing the electron transfer rate. As a result, SnO-P shows better catalytic performance than SnO. The SnO-P modified cell is designed, and it exhibits an increase of 47.2 mA h in discharge capacity and 8.7% in energy efficiency compared with the pristine cell at 150 mA cm. These increases indicate that the modified cell has a higher electrolyte utilization rate. This study shows that SnO-P is a new and efficient catalyst for vanadium redox flow battery.
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http://dx.doi.org/10.3389/fchem.2021.688634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263913PMC
June 2021

The Impact of Preoperative Sarcopenia on Survival Prognosis in Patients Receiving Neoadjuvant Therapy for Esophageal Cancer: A Systematic Review and Meta-Analysis.

Front Oncol 2021 23;11:619592. Epub 2021 Jun 23.

Department of Gastroenterology, The Affiliated hospital of Qingdao University, Qingdao, China.

Background: Sarcopenia is a poor prognostic factor in patients with esophageal cancer (EC). It can be aggravated by neoadjuvant therapy (NAT) that improves the prognosis of patients with EC. Until now, the impact of preoperative sarcopenia on survival prognosis in patients receiving NAT for EC remains unclear.

Methods: We systematically researched relevant studies in the PubMed, EMBASE, Web of Science, the Cochrane Library databases up to March 8, 2020. Prevalence of sarcopenia before and after NAT, overall survival (OS) and disease-free survival (DFS) were collected for analysis. Finally, eleven cohort studies were included.

Results: Pooled analysis indicated that preoperative sarcopenia was negatively associated with OS. ( = 1.290; 95% [1.078-1.543]; = 0.005; = 0.0%) and DFS ( = 1.554; 95% [1.177-2.052]; = 0.002; = 0.0%) in the patients with EC receiving NAT. The prevalence of sarcopenia increased by 15.4% following NAT (95% [12.9%-17.9%]). Further subgroup analysis indicated that sarcopenia diagnosed following NAT ( = 1.359; 95% [1.036-1.739]; = 0.015; = 6.9%) and age >65 years ( = 1.381; 95% [1.090- 1.749]; = 0.007; = 0.0%) were the independent risk factors for decreased OS.

Conclusions: Clinicians should strengthen the screening of preoperative sarcopenia in patients of EC both receiving NAT and older than 65 years and give active nutritional support to improve the prognosis of patients.

Systematic Review Registration: International Platform of Registered Systematic Review and Meta-Analysis Protocols (INPLASY), identifier INPLASY202050057.
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http://dx.doi.org/10.3389/fonc.2021.619592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260681PMC
June 2021

Fast and Specific Screening of EPA/DHA-Enriched Phospholipids in Fish Oil Extracted from Different Species by HILIC-MS.

J Agric Food Chem 2021 Jul 9;69(28):7997-8007. Epub 2021 Jul 9.

Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China.

Eicosapentaenoic acid- and docosahexaenoic acid-enriched phospholipids (PL) have versatile health-beneficial functions and can be well absorbed in the intestine. Herein, a precursor ion scan-driven hydrophilic interaction chromatography mass spectrometry (PreIS-HILIC-MS) method with the fatty acyl moieties of / 301.6 and 327.6 locked was established to specifically and selectively screen PL in different fish oil samples, including saury, grass carp, hairtail, and yellow croaker. Taking saury oil as an example, a total of 24 PL were successfully identified and quantified, including 20 PC and 4 PE. Finally, this method was validated in terms of sensitivity (limit of detection ≤ 4.15 μg·mL), linearity (≥0.9979), precision (RSD ≤ 4.65%), and recovery (≥78.6%). The performance of the PreIS-HILIC-MS method was also compared with that of the traditional full-scan mode, and the former demonstrated its unique superiority in targeted screening of PL in fish oils.
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http://dx.doi.org/10.1021/acs.jafc.1c01709DOI Listing
July 2021

Risk Factors of Delayed Recovery of Gastrointestinal Function After Ileostomy Reversal for Rectal Cancer Patients.

Cancer Manag Res 2021 29;13:5127-5133. Epub 2021 Jun 29.

Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, Guangdong, People's Republic of China.

Purpose: The aim of this study was to identify the risk factors associated with delayed recovery of gastrointestinal function after ileostomy reversal for rectal cancer patients.

Methods: In this retrospective study, the data of rectal cancer patients who underwent ileostomy reversal from January 2018 to December 2019 at the Sixth Affiliated Hospital of Sun Yat-sen University were assessed to investigate potential risk factors of delayed flatus after ileostomy reversal.

Results: A total of 282 patients were eligible for this study. Postoperative first flatus time ranged from 1 to 9 days, of which 58.8% patients presented with delayed flatus that was longer than 3 days. Univariate analysis showed that delayed postoperative flatus was significantly associated with the length of postoperative hospital stay (<0.001) and postoperative complications (=0.037). Multivariate analysis showed that intravenous fluid infusion at postoperative day 1 (POD1) (OR=1.001, 95% CI: 1.001-1.002, =0.001) and duration of stoma ≥6 months (OR=2.005, 95% CI:1.155-3.657, =0.014) were independent risk factors for delayed flatus.

Conclusion: Increased intravenous fluid infusion at POD1 and duration of stoma ≥6 months were related to delayed recovery of gastrointestinal function after ileostomy reversal for rectal cancer patients.
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http://dx.doi.org/10.2147/CMAR.S311715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254522PMC
June 2021

Phospholipidomics quality evaluation of swimming crabs (Portunus trituberculatus) cultured with formulated feed, frozen trash fish, and mixed feed, a non-target approach by HILIC-MS.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Aug 26;1179:122845. Epub 2021 Jun 26.

Zhejiang Province Key Laboratory of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China; Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address:

Swimming crab, Portunus trituberculatus, is popularly consumed because of its flavor and nutritional value. Herein, the crabs cultivated with formulated feed, frozen trash fish, and mixed feed were lipidomically analyzed by non-target HILIC-QTRAP/MS. The results showed that there were four phospholipid classes comprising 81 molecular species with plenty of polyunsaturated fatty acyl chains observed. The formulated feed group owned the highest content of phospholipids (332.91 μg·mg), followed by the frozen trash fish group (294.74 μg·mg) and mixed feed group (279.74 μg·mg). The effect of feeding modes was compared statistically, and the most contributing variables of m/z 802 (PC 34:2), m/z 846 (ether PC o-38:1), m/z 792 (PE 40:5), etc. were screened out and verified. The phospholipidomics results indicated that the formulated feed could replace frozen trash fish for the cultivation of P. trituberculatus.
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http://dx.doi.org/10.1016/j.jchromb.2021.122845DOI Listing
August 2021

Brain Tumor MR Image Classification Using Convolutional Dictionary Learning With Local Constraint.

Front Neurosci 2021 28;15:679847. Epub 2021 May 28.

School of Computer Science and Artificial Intelligence, Changzhou University, Changzhou, China.

Brain tumor image classification is an important part of medical image processing. It assists doctors to make accurate diagnosis and treatment plans. Magnetic resonance (MR) imaging is one of the main imaging tools to study brain tissue. In this article, we propose a brain tumor MR image classification method using convolutional dictionary learning with local constraint (CDLLC). Our method integrates the multi-layer dictionary learning into a convolutional neural network (CNN) structure to explore the discriminative information. Encoding a vector on a dictionary can be considered as multiple projections into new spaces, and the obtained coding vector is sparse. Meanwhile, in order to preserve the geometric structure of data and utilize the supervised information, we construct the local constraint of atoms through a supervised -nearest neighbor graph, so that the discrimination of the obtained dictionary is strong. To solve the proposed problem, an efficient iterative optimization scheme is designed. In the experiment, two clinically relevant multi-class classification tasks on the Cheng and REMBRANDT datasets are designed. The evaluation results demonstrate that our method is effective for brain tumor MR image classification, and it could outperform other comparisons.
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http://dx.doi.org/10.3389/fnins.2021.679847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193950PMC
May 2021

The unique molecular mechanism of diabetic nephropathy: a bioinformatics analysis of over 250 microarray datasets.

Clin Kidney J 2021 Jun 18;14(6):1626-1638. Epub 2021 Mar 18.

Department of Nephrology, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi, Jiangsu, China.

Background/aims: Diabetic nephropathy (DN) is one of the main causes of end-stage kidney disease worldwide. Emerging studies have suggested that its pathogenesis is distinct from nondiabetic renal diseases in many aspects. However, it still lacks a comprehensive understanding of the unique molecular mechanism of DN.

Methods: A total of 255 Affymetrix U133 microarray datasets (Affymetrix, Santa Calra, CA, USA) of human glomerular and tubulointerstitial tissues were collected. The 22 215 Affymetrix identifiers shared by the Human Genome U133 Plus 2.0 and U133A Array were extracted to facilitate dataset pooling. Next, a linear model was constructed and the empirical Bayes method was used to select the differentially expressed genes (DEGs) of each kidney disease. Based on these DEG sets, the unique DEGs of DN were identified and further analyzed using gene ontology and pathway enrichment analysis. Finally, the protein-protein interaction networks (PINs) were constructed and hub genes were selected to further refine the results.

Results: A total of 129 and 1251 unique DEGs were identified in the diabetic glomerulus (upregulated  = 83 and downregulated  = 203) and the diabetic tubulointerstitium (upregulated  = 399 and downregulated  = 874), respectively. Enrichment analysis revealed that the DEGs in the diabetic glomerulus were significantly associated with the extracellular matrix, cell growth, regulation of blood coagulation, cholesterol homeostasis, intrinsic apoptotic signaling pathway and renal filtration cell differentiation. In the diabetic tubulointerstitium, the significantly enriched biological processes and pathways included metabolism, the advanced glycation end products-receptor for advanced glycation end products signaling pathway in diabetic complications, the epidermal growth factor receptor (EGFR) signaling pathway, the FoxO signaling pathway, autophagy and ferroptosis. By constructing PINs, several nodes, such as AGR2, CSNK2A1, EGFR and HSPD1, were identified as hub genes, which might play key roles in regulating the development of DN.

Conclusions: Our study not only reveals the unique molecular mechanism of DN but also provides a valuable resource for biomarker and therapeutic target discovery. Some of our findings are promising and should be explored in future work.
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http://dx.doi.org/10.1093/ckj/sfaa190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162860PMC
June 2021

Single-cell analysis of pancreatic ductal adenocarcinoma identifies a novel fibroblast subtype associated with poor prognosis but better immunotherapy response.

Cell Discov 2021 May 25;7(1):36. Epub 2021 May 25.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Department of Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

The current pathological and molecular classification of pancreatic ductal adenocarcinoma (PDAC) provides limited guidance for treatment options, especially for immunotherapy. Cancer-associated fibroblasts (CAFs) are major players of desmoplastic stroma in PDAC, modulating tumor progression and therapeutic response. Using single-cell RNA sequencing, we explored the intertumoral heterogeneity among PDAC patients with different degrees of desmoplasia. We found substantial intertumoral heterogeneity in CAFs, ductal cancer cells, and immune cells between the extremely dense and loose types of PDACs (dense-type, high desmoplasia; loose-type, low desmoplasia). Notably, no difference in CAF abundance was detected, but a novel subtype of CAFs with a highly activated metabolic state (meCAFs) was found in loose-type PDAC compared to dense-type PDAC. MeCAFs had highly active glycolysis, whereas the corresponding cancer cells used oxidative phosphorylation as a major metabolic mode rather than glycolysis. We found that the proportion and activity of immune cells were much higher in loose-type PDAC than in dense-type PDAC. Then, the clinical significance of the CAF subtypes was further validated in our PDAC cohort and a public database. PDAC patients with abundant meCAFs had a higher risk of metastasis and a poor prognosis but showed a dramatically better response to immunotherapy (64.71% objective response rate, one complete response). We characterized the intertumoral heterogeneity of cellular components, immune activity, and metabolic status between dense- and loose-type PDACs and identified meCAFs as a novel CAF subtype critical for PDAC progression and the susceptibility to immunotherapy.
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http://dx.doi.org/10.1038/s41421-021-00271-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149399PMC
May 2021

Synergistic Catalysis of SnO-CNTs Composite for /VO and V/V Redox Reactions.

Front Chem 2021 5;9:671575. Epub 2021 May 5.

School of Chemical Engineering, North China University of Science and Technology, Tangshan, China.

In this study, a SnO-carbon nanotube (SnO-CNT) composite as a catalyst for vanadium redox flow battery (VRFB) was prepared using a sol-gel method. The effects of this composite on the electrochemical performance of /VO, and on the V/V redox reactions and VRFB performance were investigated. The SnO-CNT composite has better catalytic activity than pure SnO and CNT due to the synergistic catalysis of SnO and the CNT. SnO mainly provides the catalytic active sites and the CNTs mainly provide the three-dimensional structure and high electrical conductivity. Therefore, the SnO-CNT composite has a larger specific surface area and an excellent synergistic catalytic performance. For cell performance, it was found that the SnO-CNT cell shows a greater discharge capacity and energy efficiency. In particular, at 150 mA cm, the discharge capacity of the SnO-CNT cell is 28.6 mAh higher than that of the pristine cell. The energy efficiency of the modified cell (7%) is 7.2% higher than that of the pristine cell (62.8%). This study shows that the SnO-CNT is an efficient and promising catalyst for VRFB.
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http://dx.doi.org/10.3389/fchem.2021.671575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131550PMC
May 2021

Sequential infection with H1N1 and SARS-CoV-2 aggravated COVID-19 pathogenesis in a mammalian model, and co-vaccination as an effective method of prevention of COVID-19 and influenza.

Signal Transduct Target Ther 2021 05 20;6(1):200. Epub 2021 May 20.

Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, NHC Key Laboratory of Human Disease Comparative Medicine, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing, China.

Influenza A virus may circulate simultaneously with the SARS-CoV-2 virus, leading to more serious respiratory diseases during this winter. However, the influence of these viruses on disease outcome when both influenza A and SARS-CoV-2 are present in the host remains unclear. Using a mammalian model, sequential infection was performed in ferrets and in K18-hACE2 mice, with SARS-CoV-2 infection following H1N1. We found that co-infection with H1N1 and SARS-CoV-2 extended the duration of clinical manifestation of COVID-19, and enhanced pulmonary damage, but reduced viral shedding of throat swabs and viral loads in the lungs of ferrets. Moreover, mortality was increased in sequentially infected mice compared with single-infection mice. Compared with single-vaccine inoculation, co-inoculation of PiCoVacc (a SARS-CoV-2 vaccine) and the flu vaccine showed no significant differences in neutralizing antibody titers or virus-specific immune responses. Combined immunization effectively protected K18-hACE2 mice against both H1N1 and SARS-CoV-2 infection. Our findings indicated the development of systematic models of co-infection of H1N1 and SARS-CoV-2, which together notably enhanced pneumonia in ferrets and mice, as well as demonstrated that simultaneous vaccination against H1N1 and SARS-CoV-2 may be an effective prevention strategy for the coming winter.
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http://dx.doi.org/10.1038/s41392-021-00618-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134832PMC
May 2021

Vitamin D receptor gene polymorphisms and risk of intervertebral disc degeneration: An updated meta-analysis based on 23 studies.

Medicine (Baltimore) 2021 May;100(20):e25922

From the Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu, China.

Background: Numerous studies have investigated the associations between Vitamin D receptor (VDR) gene polymorphisms and risk of intervertebral disc degeneration but the results remain controversial. This study aimed to drive a more precise estimation of association between VDR gene polymorphisms and risk of intervertebral disc degeneration.

Methods: PubMed, EMBASE, Cochrane library, Web of Science and China Knowledge Resource Integrated Database for papers on VDR gene polymorphisms and risk of intervertebral disc degeneration were searched. The pooled odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of association in the homozygote model, heterozygote model, dominant model, recessive model and an additive model.

Results: Overall, 23 articles were included in the final meta-analysis. The subgroup analyses by ethnicity showed a significant association of VDR FokI mutation with disc degeneration risk in Caucasians (recessive model, OR with 95%CI 1.301, [1.041, 1.626]; additive model, OR with 95%CI 1.119, [1.006, 1.245]). The results of subgroup analyses by ethnicity showed a significant association of VDR TaqI mutation with disc degeneration risk in Asians but not in Caucasians. There was a significant association between VDR ApaI mutation and risk of disc degeneration and subgroup analyses by ethnicity showed a significant association in Caucasians and in Asians.

Conclusions: In summary, VDR FokI polymorphisms was associated with disc degeneration risk among Caucasians but not Asians, VDR TaqI polymorphisms was associated with disc degeneration risk among Asians but not Caucasians, while VDR ApaI polymorphism was associated with disc degeneration risk among Asians and Caucasians.
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http://dx.doi.org/10.1097/MD.0000000000025922DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136998PMC
May 2021

Caulobacter endophyticus sp. nov., an endophytic bacterium harboring three lasso peptide biosynthetic gene clusters and producing indoleacetic acid isolated from maize root.

Antonie Van Leeuwenhoek 2021 Aug 18;114(8):1213-1224. Epub 2021 May 18.

Key Laboratory of Microbial Resources, Ministry of Agriculture and Rural Affairs/ Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.

A novel Gram-stain-negative, aerobic and rod-shaped bacterium with a single polar flagellum or a stalk at the end of the cell, was isolated from maize roots in the Fangshan District of Beijing, People's Republic of China. The new strain designated 774 produced indole acetic acid (IAA). The 16S rRNA gene sequence analysis indicated that strain 774 belongs to the genus Caulobacter and is closely related to Caulobacter flavus RHGG3, Caulobacter zeae 410and Caulobacter radices 695, all with sequence similarities of 99.9%. The genome size of strain774 was 5.4 Mb, comprising 5042 predicted genes with a DNA G+C content of 68.7%.Three striking lasso peptide biosynthetic gene clusters and two IAA synthesis genes belonging to the TPM pathway were also found in the genome of strain 774. The average nucleotide identity values and digital DNA-DNA hybridization values of the strain774 with its closely phylogenetic neighbours were less than 91.5% and 45.0%, respectively, indicating a new Caulobacter species. The major fatty acids of strain774 were identified as C16: 0 (27.7%), summed feature 3 (C16: 1ω6c and/or C16: 1ω7c) (12.6%) and summed feature 8 (C18: 1ω7c and/or C18: 1ω6c) (42.9%).The major polar lipids consisted of phosphatidyl-glycerol and glycolipids. The predominant ubiquinone was identified as Quinone 10. Based on the polyphasic characterization, strain 774 represents a novel species of the genus Caulobacter, for which the name Caulobacter endophyticus sp. nov. is proposed with 774 (= CGMCC 1.16558 = DSM 106777) as the type strain.
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http://dx.doi.org/10.1007/s10482-021-01593-9DOI Listing
August 2021
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