Publications by authors named "Jing Xu"

3,284 Publications

  • Page 1 of 1

MicroRNA-423-3p promotes glioma growth by targeting PANX2.

Oncol Lett 2021 Aug 2;22(2):572. Epub 2021 Jun 2.

[This retracts the article DOI: 10.3892/ol.2018.8636.].
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http://dx.doi.org/10.3892/ol.2021.12833DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185900PMC
August 2021

Metronomic capecitabine as adjuvant therapy in locoregionally advanced nasopharyngeal carcinoma: a multicentre, open-label, parallel-group, randomised, controlled, phase 3 trial.

Lancet 2021 Jun 4. Epub 2021 Jun 4.

Department of Radiation Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangzhou, China.

Background: Patients with locoregionally advanced nasopharyngeal carcinoma have a high risk of disease relapse, despite a high proportion of patients attaining complete clinical remission after receiving standard-of-care treatment (ie, definitive concurrent chemoradiotherapy with or without induction chemotherapy). Additional adjuvant therapies are needed to further reduce the risk of recurrence and death. However, the benefit of adjuvant chemotherapy for nasopharyngeal carcinoma remains controversial, highlighting the need for more effective adjuvant treatment options.

Methods: This multicentre, open-label, parallel-group, randomised, controlled, phase 3 trial was done at 14 hospitals in China. Patients (aged 18-65 years) with histologically confirmed, high-risk locoregionally advanced nasopharyngeal carcinoma (stage III-IVA, excluding T3-4N0 and T3N1 disease), no locoregional disease or distant metastasis after definitive chemoradiotherapy, an Eastern Cooperative Oncology Group performance status of 0 or 1, sufficient haematological, renal, and hepatic function, and who had received their final radiotherapy dose 12-16 weeks before randomisation, were randomly assigned (1:1) to receive either oral metronomic capecitabine (650 mg/m body surface area twice daily for 1 year; metronomic capecitabine group) or observation (standard therapy group). Randomisation was done with a computer-generated sequence (block size of four), stratified by trial centre and receipt of induction chemotherapy (yes or no). The primary endpoint was failure-free survival, defined as the time from randomisation to disease recurrence (distant metastasis or locoregional recurrence) or death due to any cause, in the intention-to-treat population. Safety was assessed in all patients who received at least one dose of capecitabine or who had commenced observation. This trial is registered with ClinicalTrials.gov, NCT02958111.

Findings: Between Jan 25, 2017, and Oct 25, 2018, 675 patients were screened, of whom 406 were enrolled and randomly assigned to the metronomic capecitabine group (n=204) or to the standard therapy group (n=202). After a median follow-up of 38 months (IQR 33-42), there were 29 (14%) events of recurrence or death in the metronomic capecitabine group and 53 (26%) events of recurrence or death in the standard therapy group. Failure-free survival at 3 years was significantly higher in the metronomic capecitabine group (85·3% [95% CI 80·4-90·6]) than in the standard therapy group (75·7% [69·9-81·9]), with a stratified hazard ratio of 0·50 (95% CI 0·32-0·79; p=0·0023). Grade 3 adverse events were reported in 35 (17%) of 201 patients in the metronomic capecitabine group and in 11 (6%) of 200 patients in the standard therapy group; hand-foot syndrome was the most common adverse event related to capecitabine (18 [9%] patients had grade 3 hand-foot syndrome). One (<1%) patient in the metronomic capecitabine group had grade 4 neutropenia. No treatment-related deaths were reported in either group.

Interpretation: The addition of metronomic adjuvant capecitabine to chemoradiotherapy significantly improved failure-free survival in patients with high-risk locoregionally advanced nasopharyngeal carcinoma, with a manageable safety profile. These results support a potential role for metronomic chemotherapy as an adjuvant therapy in the treatment of nasopharyngeal carcinoma.

Funding: The National Natural Science Foundation of China, the Key-Area Research and Development Program of Guangdong Province, the Natural Science Foundation of Guangdong Province, the Innovation Team Development Plan of the Ministry of Education, and the Overseas Expertise Introduction Project for Discipline Innovation.

Translation: For the Chinese translation of the abstract see Supplementary Materials section.
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http://dx.doi.org/10.1016/S0140-6736(21)01123-5DOI Listing
June 2021

Coherent Gate Operations in Hybrid Magnonics.

Phys Rev Lett 2021 May;126(20):207202

Center for Nanoscale Materials, Argonne National Laboratory, Lemont, Illinois 60439, USA.

Electromagnonics-the hybridization of spin excitations and electromagnetic waves-has been recognized as a promising candidate for coherent information processing in recent years. Among its various implementations, the lack of available approaches for real-time manipulation on the system dynamics has become a common and urgent limitation. In this work, by introducing a fast and uniform modulation technique, we successfully demonstrate a series of benchmark coherent gate operations in hybrid magnonics, including semiclassical analogies of Landau-Zener transitions, Rabi oscillations, Ramsey interference, and controlled mode swap operations. Our approach lays the groundwork for dynamical manipulation of coherent signals in hybrid magnonics and can be generalized to a broad range of applications.
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http://dx.doi.org/10.1103/PhysRevLett.126.207202DOI Listing
May 2021

Immediate Analgesic Effect of Acupuncture in Patients With Primary Dysmenorrhea: A fMRI Study.

Front Neurosci 2021 24;15:647667. Epub 2021 May 24.

Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Primary dysmenorrhea (PDM) is a common gynecological disease characterized by lower abdominal pain. Acupuncture is considered a good alternative therapy for PDM. However, the central mechanism of the analgesic effect of acupuncture is largely unknown. In this study, eligible patients were randomized into the real and sham acupuncture groups using a computer-generated, permuted block randomization method. The study cohort comprised 34 patients: 19 in the real acupuncture group and 15 in the sham acupuncture group. The clinical characteristics of the patients during their menstrual period were collected, and imaging scans were performed during the first 3 days of the patients' menstrual period. We analyzed task and resting functional magnetic resonance imaging (fMRI) data to investigate the potential central mechanism of the immediate effect of acupuncture intervention on the intensity of PDM pain. The task fMRI study found that the rostral anterior cingulate cortex (rACC) and right supplemental motor area were activated during real acupuncture. Using the resting-state functional connectivity (FC) method, we found a post- versus pre-treatment change in the FC of the rACC and left precentral gyrus in the comparison of real acupuncture versus sham acupuncture. In addition, the FC of the rACC-left precentral gyrus at baseline was negatively correlated with short-term analgesia, while the change in the FC of the rACC-left precentral gyrus was positively correlated with short-term analgesia after acupuncture treatment. These findings support the importance of rACC-left precentral gyrus resting-state FC in the modulation of the intensity of PDM pain through acupuncture, which may shed light on the central mechanism of acupuncture in the treatment of PDM.
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http://dx.doi.org/10.3389/fnins.2021.647667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180846PMC
May 2021

Intraoperative Neuromonitoring Auxiliary Significance of DNEP for MEP-positive Event During Severe Spinal Deformity Surgery.

Clin Spine Surg 2021 Jun 9. Epub 2021 Jun 9.

Spine Center, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai Department of Orthopaedic Surgery, the 1st Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China.

Study Design: This was a retrospective analysis.

Objective: The objective of this study was to assess the intraoperative neuromonitoring auxiliary significance of descending neurogenic-evoked potential (DNEP) for motor-evoked potential (MEP) during severe spinal deformity surgery when MEP-positive event occurs.

Summary Of Background Data: MEP detection is the most widely applied neurological monitoring technique in spinal deformity surgery. MEP is quite vulnerable to anesthesia, blood pressure, and other intraoperative factors, leading to a high false-positive rate of MEP (3.2%-45.0%), which has greatly interfered with the surgical process. At present, the widely used "presence-or-absence" alarm criteria of MEP is not enough to solve the problem of false positive of MEP.

Methods: A total of 205 cases undergoing severe spinal deformity correction were retrospectively studied. Overall, 74 MEP-positive cases were classified as 2 subgroups: DNEP (+) and DNEP (-) groups. The MEP recovery, wake-up test, and Frankle grade were used to assess the neurological functions. The perioperative and long-term neurological outcomes were assessed.

Results: There were significant differences in preoperative scoliosis angle and kyphosis angle between DNEP (-) and DNEP (+) groups. Patients in DNEP (-) group showed more MEP improvement (81.5%), compared with the DNEP (+) group (53.2%). The Wake-up test showed 59.3% motor function deficit cases in DNEP (-) group, which was lower than the 87.2% in DNEP (+) group. More patients in DNEP (-) group had normal nerve function (Frankel level E) than those in DNEP (+) group immediately after surgery, as well as at follow-up.

Conclusions: MEP-positive cases with intraoperative DNEP (-) showed superior prognosis after severe spinal deformity surgery. Intraoperative DNEP could be regarded as an important quantitative tool to assist MEP to monitor neurological injury and can serve as a temporary substitution monitoring technique after MEP is lost.
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http://dx.doi.org/10.1097/BSD.0000000000001190DOI Listing
June 2021

Diagnostic value of NPTX2 (neuronal pentraxin II) methylation in patients with pancreatic cancer: meta-analysis.

Int J Clin Pract 2021 Jun 9:e14443. Epub 2021 Jun 9.

Department of Gastroenterology, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, 510180, China.

Pancreatic cancer (PC) is a devasting disease of which mortality almost parallels its incidence. Pancreatic cancer tissue may express aberrantly methylated NPTX2, but it is unclear what the consequences of this are. We analysed 9 studies, with a total of 927 patients, to identify if the detection of methylated NPTX2 have sufficient sensitivity and specificity to identify pancreatic cancer from other benign pancreatic diseases. The majority studies obtained samples from pancreatic juice by endoscopy or surgery and composed of population with chronic pancreatitis, benign cystic lesion, intraductal papillary mucinous neoplasm, healthy controls. Our results demonstrated that the diagnostic value of methylated NPTX2 are of widely various sensitivity and specificity and it shown higher specificity in differentiate PC from benign diseases. The lab method of QMSP has higher specificity than MSP in detecting the indicator. The variable sensitivity of methylated NPTX2 was multifactorial and it must be promoted before applied as screening test in clinical practice.
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http://dx.doi.org/10.1111/ijcp.14443DOI Listing
June 2021

Multi-scale hybrid modified coatings on titanium implants for non-cytotoxicity and antibacterial properties.

Nanoscale 2021 Jun 8. Epub 2021 Jun 8.

State Key Laboratory of Metal Matrix Composites, School of Material Science and Engineering, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai, 200240, China. and Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.

Titanium and its alloys are among the widely used materials in the biomedical field, but they have poor wear resistance and antibacterial properties. In the present study, anodization, photo-reduction, and spin-coating technologies were integrated to prepare a hybrid modified coating for bio-inert titanium implants, having excellent comprehensive performance. The surface roughness of Ti-35Nb-2Ta-3Zr was specifically optimized by surface modification leading to improved wear resistance. Ag ions are still detectable after 28 days of submersion in saline. The antibacterial rate of the composite coating group reaches 100% by plate counting due to the antibacterial mechanism of direct and indirect contact. Both bacteria morphology and fluorescence staining experiments confirm these results. Besides, no cytotoxicity was detected in our fabricated implants during the CCK-8 assay. Accordingly, fabrication of hybrid modified coatings on Ti-35Nb-2Ta-3Zr is an effective strategy for infection and cytotoxicity prevention. These hybrid modified coatings can be regarded as promising multifunctional biomaterials.
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http://dx.doi.org/10.1039/d1nr02459kDOI Listing
June 2021

[Quantitative Analysis on Immunophenotype of CD34 Myeloid Precursor Cells in Myelodysplastic Syndrome and Its Correlation with Clinical Features].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):832-839

Department of Hematology, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China,E-mail:

Objective: To investigate the quantitative expression of immunophenotype of CD34 myeloid precursor cells in myelodysplastic syndrome (MDS) patients and its correlation with clinical characteristics, and understand the effect of quantitative expression of CD7 and CD117 on the prognosis of low-risk MDS patients.

Methods: Multi-parameter flow cytometry (FCM) was used to detect the proportion and mean fluorescence intensity (MFI) of each antigen of bone marrow CD34 myeloid precursor cells in 79 MDS patients. The correlation between the expression level of each immune marker and clinical characteristics was compared. The effects of quantitative expressions of CD7 and CD117 on the overall survival rate of low-risk patients were explored.

Results: Bone marrow blast cell proportion (P<0.01), RBC level (P<0.01), and Hb level (P<0.05) of high-risk MDS patients were higher, while EPO level (P<0.05) was lower than those of low-risk patients. The proportion of CD34 blast cells (P<0.01), the proportion of CD117 (P<0.05) and the MFI of CD7 (P<0.05) were higher in high-risk patients than those in low-risk patients, but the MFI of CD123 was lower (P<0.05). In high-risk MDS patients, CD15/CD34 (MFI) and CD19/CD34 (MFI) positively correlated with the proportion of total T cells (r=0.458; r=0.505), while CD19/CD34 (%) and CD19/CD34 (MFI) negatively correlated with WBC levels (r=-0.469; r=-0.503). In low-risk MDS patients, CD34 (%) positively correlated with bone marrow erythrocyte proportion, PLT level and neutrophil level (r=0.426; r=0.486; r=0.495), but negatively correlated with LDH level (r=-0.421); WT1 expression level was positively correlated with CD10/CD34 (%), CD10/CD34 (MFI) and CD117/CD34 (MFI) (r=0.745; r=0.800; r=0.434), while negatively correlated with CD11b/CD34 (%)(r=-0.457); CD19/CD34 (%) and CD71/CD34 (MFI) negatively correlated with NK cell proportion (r=-0.786; r=-0.514); CD10/CD34 (%) positively correlated with Th/Ts, while CD7/CD34 (MFI) negatively correlated with the proportion of Th cells (r=0.738; r=-0.513); HLADR/CD34 (%) and HLADR/CD34 (MFI) negatively correlated with PLT level (r=-0.461; r=-0.445), while HLADR/CD34 (MFI) positively correlated with bone marrow NAP fraction (r=0.552). The quantitative expression of CD7 and CD117 had no significant effect on the overall survival rate of low-risk MDS patients.

Conclusion: The immunophenotype of CD34 myeloid precursor cell in different risk groups in MDS patients is related to clinical characteristics. Bone marrow cell morphology, clinical and laboratory features and immunophenotype will be of great significance to the diagnosis, clinical classification and prognosis evaluation of MDS patients.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.03.028DOI Listing
June 2021

Global transcriptome landscape of the rabbit protozoan parasite Eimeria stiedae.

Parasit Vectors 2021 Jun 7;14(1):308. Epub 2021 Jun 7.

Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.

Background: Coccidiosis caused by Eimeria stiedae is a widespread and economically significant disease of rabbits. The lack of studies on the life-cycle development and host interactions of E. stiedae at the molecular level has hampered our understanding of its pathogenesis.

Methods: In this study, we present a comprehensive transcriptome landscape of E. stiedae to illustrate its dynamic development from unsporulated oocysts to sporulated oocysts, merozoites, and gametocytes, and to identify genes related to parasite-host interactions during parasitism using combined PacBio single-molecule real-time and Illumina RNA sequencing followed by bioinformatics analysis and qRT-PCR validation.

Results: In total, 12,582 non-redundant full-length transcripts were generated with an average length of 1808 bp from the life-cycle stages of E. stiedae. Pairwise comparisons between stages revealed 8775 differentially expressed genes (DEGs) showing highly significant description changes, which compiled a snapshot of the mechanisms underlining asexual and sexual biology of E. stiedae including oocyst sporulation between unsporulated and sporulated oocysts; merozoite replication between sporulated oocysts and merozoites; and gametophyte development and gamete generation between merozoites and gametocytes. Further, 248 DEGs were grouped into nine series clusters and five groups by expression patterns, and showed that parasite-host interaction-related genes predominated in merozoites and gametocytes and were mostly involved in steroid biosynthesis and lipid metabolism and carboxylic acid. Additionally, co-expression analyses identified genes associated with development and host invasion in unsporulated and sporulated oocysts and immune interactions during gametocyte parasitism.

Conclusions: This is the first study, to our knowledge, to use the global transcriptome profiles to decipher molecular changes across the E. stiedae life cycle, and these results not only provide important information for the molecular characterization of E. stiedae, but also offer valuable resources to study other apicomplexan parasites with veterinary and public significance.
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http://dx.doi.org/10.1186/s13071-021-04811-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186055PMC
June 2021

Adsorption-Reaction Processes Between Gelatin and PDMS-E Emulsion Droplets.

ACS Omega 2021 Jun 20;6(21):13915-13925. Epub 2021 May 20.

Shandong Provincial Key Laboratory of Molecular Engineering, School of Mathematics and Statistics, Qilu University of Technology Library, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, P. R. China.

In this work, the effects of droplet size and reaction time on the adsorption-reaction processes between gelatin and α-[3-(2,3-epoxypropoxy) propyl]-ω-butyl-polydimethylsiloxane (PDMS-E) emulsion droplets were studied. Gelatin molecules were only physically adsorbed on the surface of the PDMS-E droplet in the 0-75 min range, which was unrelated to the droplet size (100-1000 nm). For the small-size droplets (<410 nm), the physical adsorption proceeded over 75 min followed by agglomeration. For middle-size droplets (410-680 nm), the physical adsorption finished at 125 min; subsequently, the nucleophilic reaction between the primary amino group and the epoxy group began to happen, and globular-like or core-shell colloidal particles were formed. For large-size droplets (>680 nm), the nucleophilic reaction occurred at 75 min and produced core-shell or multi-layered colloidal particles. In a word, the physical absorption or nucleophilic reaction between gelatin and PDMS-E emulsion droplets could be controlled by controlling the droplet size and reaction time. Furthermore, the soft tissue paper coated with large-size droplets exhibited excellent resistance to water permeability and flame-resistant performance, which were carried out by water resistance and flammability tests.
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http://dx.doi.org/10.1021/acsomega.1c01789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173617PMC
June 2021

Efficacy and safety of rituximab in adult frequent-relapsing or steroid-dependent minimal change disease or focal segmental glomerulosclerosis: a systematic review and meta-analysis.

Clin Kidney J 2021 Apr 21;14(4):1042-1054. Epub 2020 Nov 21.

Division of Nephrology, Kidney Institute of CPLA (Chinese People's Liberation Army), Changzheng Hospital, Second Military Medical University, Shanghai, China.

Background: The efficacy and safety of rituximab (RTX) in adult frequent-relapsing (FR) or steroid-dependent (SD) nephrotic syndrome (NS), including minimal change disease (MCD) or focal segmental glomerulosclerosis (FSGS), are still inconclusive.

Methods: We performed a systematic review and meta-analysis registered in  PROSPERO (CRD42019148102) by pooling data of cohort studies or case series on adult patients with difficult-to-treat NS. Steroid-resistant NS was excluded. The primary outcomes were the complete remission (CR) rate and the relapse rate. Partial remission (PR) rate, no response (NR) rate and adverse events were the secondary outcomes. A random-effects model was performed for all the outcomes.

Results: We included 21 studies involving 382 adult MCD/FSGS subjects with a median follow-up duration from 12 to 43 months. RTX treatment induced a pooled 84.2% CR rate [95% confidence interval (CI): 67.7-96.3%], while MCD patients had a high 91.6% CR rate and FSGS patients a moderate 43% CR rate. However, 27.4% (95% CI 20.7-34.5%) of the patients relapsed during the follow-up. The pooled PR and NR rates were 5.8% (95% CI 1.2-12.5%) and 5.2% (95% CI 0.0-15.0%), respectively. RTX was associated with trivial adverse events and good tolerance.

Conclusions: In summary, by pooling results of current pilot studies, RTX may be an effective and relatively safe alternative for most adult FR or SD MCD/FSGS to displace calcineurin inhibitors or prednisone in the hierarchy of treatment. More clinical trials comparing RTX with other immunosuppressants and concerning the long-term adverse events are needed.
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http://dx.doi.org/10.1093/ckj/sfaa191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173623PMC
April 2021

Magnetic covalent organic framework nanocomposites as a new adsorbent for the determination of polycyclic aromatic hydrocarbons in water and food samples.

Anal Methods 2021 Jun 4. Epub 2021 Jun 4.

School of Chemistry and Materials Science of Shanxi Normal University, Key Laboratory of Magnetic Molecules and Magnetic Information Materials of Ministry of Education, Linfen 041004, China.

A magnetic covalent organic framework nanocomposite ([email protected](Tp-NDA)) was synthesized via a solvothermal method, used as a magnetic adsorbent for the extraction of polycyclic aromatic hydrocarbons (PAHs) from lake water, tea, coffee, and fried chicken, and detected using a high performance liquid chromatography-ultraviolet detector. The synthesized magnetic adsorbent was characterized via transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, N2 adsorption-desorption isotherm analysis and vibrating sample magnetometry. Parameters that affected the extraction conditions and desorption conditions were optimized. Adsorption equilibrium could be attained within 3 min. The prepared magnetic material could be reused 10 times. The limits of detection and quantification were 0.05-0.25 μg L-1 and 0.17-0.83 μg L-1, respectively. The recovery was 74.6-101.8% with a relative standard deviation of below 4.2%. The method was successfully used to detect PAHs in various samples.
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http://dx.doi.org/10.1039/d1ay00496dDOI Listing
June 2021

[Effect of electroacupuncture at "Weizhong" (BL40) on expression of muscular PDGF-CC, PDGFR-α and MMP-1 in rats with lumbar multifidus muscle injury].

Zhen Ci Yan Jiu 2021 May;46(5):397-403

Department of Acupuncture and Rehabilitation, Guangdong Second Hospital of Traditional Chinese Medicine, Guangzhou 510095.

Objective: To observe the effect of electroacupuncture (EA) of "Weizhong" (BL40) on the expression of platelet-derived growth factor (PDGF)-CC, PDGF receptor (PDGFR)α and matrix metalloproteinase-1 (MMP-1) in rats with lumbar multifidus muscle injury (LMMI) so as to study its mechanisms underlying improvement of skeletal muscle injury.

Methods: Fifty-four male SD rats were randomly divided into normal group (=6), model group (=24) and EA group (=24), and the latter two groups were further divided into four subgroups (1, 3, 5 and 7 days), with 6 rats in each group. The LMMI model was established by injection of 0.5% bupivacaine (BPVC, 100 μL×4) into the multifidus along the L4 and L5 spinous process. EA (2 Hz/50 Hz, 1 mA) was applied to bilateral "Weizhong"(BL40) for 20 min, once daily for 1, 3, 5 and 7 days respectively, from the first day on after modeling. Histopathological changes of the left multifidus muscle were observed after H.E. staining, and the expression of PDGF-CC, PDGFR-α and MMP-1 proteins in the right multifidus was observed by Western blot.

Results: Compared with the normal group, the expression levels of PDGF-CC protein in the model subgroup 1 d, 3 d and 7 d were significantly decreased (<0.05), and those of PDGFR-α and MMP-1 proteins in the model subgroup 5 d and 7 d, and PDGF-CC protein in the model subgroup 5 d significantly increased (<0.05). In comparison with the model subgroups, the expression levels of PDGF-CC in the EA subgroup 3 d, 5 d and 7 d, PDGFR-α in the EA subgroup 5 d, and MMP-1 in the EA group 3 d and 5 d were significantly increased or significantly further increased (<0.05). H.E. staining showed different shapes and uneven sizes, with large area of damage, enlarged muscle space and inflammatory cell infiltration in the model group, which was relatively milder in the EA subgroups particularly in subgroup 5 d and 7 d.

Conclusion: EA stimulation of BL40 for about 5 days has a positive effect in promoting the repair of the injured multifidus muscle in LMMI rats, which may be related to its function in up-regulating the expression of muscular PDGF-CC, PDGFR-α and MMP-1 proteins.
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http://dx.doi.org/10.13702/j.1000-0607.201198DOI Listing
May 2021

MS-ANet: deep learning for automated multi-label thoracic disease detection and classification.

Authors:
Jing Xu Hui Li Xiu Li

PeerJ Comput Sci 2021 17;7:e541. Epub 2021 May 17.

Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, China.

The chest X-ray is one of the most common radiological examination types for the diagnosis of chest diseases. Nowadays, the automatic classification technology of radiological images has been widely used in clinical diagnosis and treatment plans. However, each disease has its own different response characteristic receptive field region, which is the main challenge for chest disease classification tasks. Besides, the imbalance of sample data categories further increases the difficulty of tasks. To solve these problems, we propose a new multi-label chest disease image classification scheme based on a multi-scale attention network. In this scheme, multi-scale information is iteratively fused to focus on regions with a high probability of disease, to effectively mine more meaningful information from data. A novel loss function is also designed to improve the rationality of visual perception and multi-label image classification, which forces the consistency of attention regions before and after image transformation. A comprehensive experiment was carried out on the Chest X-Ray14 and CheXpert datasets, separately containing over 100,000 frontal-view and 200,000 front and side view X-ray images with 14 diseases. The AUROC is 0.850 and 0.815 respectively on the two data sets, which achieve the state-of-the-art results, verified the effectiveness of this method in chest X-ray image classification. This study has important practical significance for using AI algorithms to assist radiologists in improving work efficiency and diagnostic accuracy.
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http://dx.doi.org/10.7717/peerj-cs.541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157016PMC
May 2021

Patients who do not fulfill criteria for hypertrophic cardiomyopathy but have unexplained giant T-wave inversion: a cardiovascular magnetic resonance mid-term follow-up study.

J Cardiovasc Magn Reson 2021 Jun 3;23(1):67. Epub 2021 Jun 3.

Department of Magnetic Resonance Imaging, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishi Road, Xicheng District, Beijing, 100037, China.

Background: Patients who have unexplained giant T-wave inversions but do not meet criteria for hypertrophic cardiomyopathy (HCM) (left ventricular (LV) wall thickness < 1.5 cm) demonstrate LV apical morphological features that differ from healthy subjects. Currently, it remains unknown how the abnormal LV apical morphology in this patient population changes over time. The purpose of this study was to investigate LV morphological and functional changes in these patients using a mid-term cardiovascular magnetic resonance (CMR) exam.

Methods: Seventy-one patients with unexplained giant T-wave inversion who did not fulfill HCM criteria were studied. The mean interval time of the follow-up CMR was 24.4 ± 8.3 months. The LV wall thickness was measured in each LV segment according to the American Heart Association 17-segmented model. The apical angle (ApA) was also measured. A receiver operating curve (ROC) was used to identify the predictive values of the CMR variables.

Results: Of 71 patients, 16 (22.5%) progressed to typical apical HCM, while 55 (77.5%) did not progress to HCM criteria. The mean apical wall thickness was significantly different between the two groups at both baseline and follow-up, with the apical HCM group having greater wall thickness at both time points (all p < 0.001). There was a significant difference between the two groups in the change of ApA (- 1.5 ± 2.7°/yr vs. - 0.7 ± 2.0°/yr, p < 0.001) over time. The combination of mean apical wall thickness and ApA proved to be the best predictor for fulfilling criteria for apical HCM with a threshold value of 8.1 mm and 90° (sensitivity 93.8%, specificity 85.5%).

Conclusions: CMR metrics identify predictors for progression to HCM in patients with unexplained giant T-wave inversion.
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http://dx.doi.org/10.1186/s12968-020-00700-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173876PMC
June 2021

Genetic basis of kernel starch content decoded in a maize multi-parent population.

Plant Biotechnol J 2021 Jun 2. Epub 2021 Jun 2.

State Key Laboratory of Plant Physiology and Biochemistry, National Maize Improvement Center of China, MOA Key Lab of Maize Biology, China Agricultural University, Beijing, 100193, China.

Starch is the most abundant storage carbohydrate in maize kernels and provides calories for humans and other animals as well as raw materials for various industrial applications. Decoding of the genetic basis of natural variation in kernel starch content is needed to manipulate starch quantity and quality via molecular breeding to meet future needs. Here, we identified 50 unique single quantitative trait loci (QTLs) for starch content with 18 novel QTLs via single linkage mapping, joint linkage mapping, and a genome-wide association study in a multi-parent population containing six recombinant inbred line populations. Only five QTLs explained over 10% of phenotypic variation in single populations. In addition to a few large-effect and many small-effect additive QTLs, limited pairs of epistatic QTLs also contributed to the genetic basis of the variation in kernel starch content. A regional association study identified five non-starch-pathway genes that were the causal candidate genes underlying the identified QTLs for starch content. The pathway-driven analysis identified ZmTPS9, which encodes a trehalose-6-phosphate synthase in the trehalose pathway, as the causal gene for the QTL qSTA4-2, which was detected by all three statistical analyses. Knockout of ZmTPS9 increased kernel starch content and, in turn, kernel weight in maize, suggesting potential applications for ZmTPS9 in maize starch and yield improvement. These findings extend our knowledge about the genetic basis of starch content in maize kernels and provide valuable information for maize genetic improvement of starch quantity and quality.
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http://dx.doi.org/10.1111/pbi.13645DOI Listing
June 2021

LINC00202 attenuates the progression of gastric cancer via suppressing expression level of KLF2.

J BUON 2021 Mar-Apr;26(2):506-512

Department of Oncology, Tongren People's Hospital, Tongren 554300, China.

Purpose: We aimed to investigate the function of LINC00202 in influencing the malignant progression of gastric cancer (GC).

Methods: The relative level of LINC00202 in GC and adjacent normal tissues was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The difference in LINC00202 level among GC patients with different TNM stages was compared. Subsequently, regulatory effects of LINC00202 on proliferative capacity of AGS and SGC-7901 cells were evaluated. Subcellular distribution of LINC00202 was analyzed. The interaction and correlation between LINC00202 and Krüppel-like factor 2 (KLF2) were analyzed by RNA immunoprecipitation (RIP), Chromatin immunoprecipitation (ChIP) and linear regression test. Finally, the involvement of KLF2 in LINC00202-mediated proliferative ability of GC cells was clarified. LINC00202 was upregulated in GC tissues compared with that in adjacent normal ones. Its level remained higher in GC patients with stage III-IV than those with stage I-II. Silencing LINC00202 markedly attenuated the proliferation of GC cells.

Results: LINC00202 was mainly enriched in nucleus. KLF2 level was negatively correlated to and interacted with LINC00202. Transfection of LINC00202 decreased the recruitment ability of EZH2 on KLF2. Importantly, KLF2 partially reversed the regulatory effect of LINC00202 on the proliferative ability of GC cells.

Conclusions: LINC00202 enhances the proliferative ability of GC cells via negatively regulating KLF2, thus aggravating the progression of GC.
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June 2021

Fat mass and obesity-associated protein (FTO) mediates signal transducer and activator of transcription 3 (STAT3)-drived resistance of breast cancer to doxorubicin.

Bioengineered 2021 Dec;12(1):1874-1889

Department of Dermatology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, the First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, China.

Excessive activation of signal transducer and activator of transcription 3 (STAT3) is implicated in breast cancer (BC) chemoresistance, but its underlying mechanism is not fully understood. There are STAT3 binding sites in fat mass and obesity-associated protein (FTO) promoter region, thus STAT3 may regulate the transcription of FTO. This study aimed to investigate the correlation between FTO and STAT3 in BC chemoresistance. Herein, FTO and STAT3 were highly expressed in doxorubicin-resistant BC (BC-DoxR) cells. CHIP assay verified the binding between STAT3 and FTO promoter in BC-DoxR cells. Dual luciferase reporter assay showed that FTO promoter activity was inhibited by S3I-201 (STAT3 inhibitor) but enhanced by epidermal growth factor (EGF, STAT3 activator) in BC-DoxR and BC cells. FTO mRNA and protein expression were suppressed by S3I-201 in BC-DoxR cells and EGF-stimulated BC cells. Notably, FTO regulated total N6-methyladenosine (m6A) levels in BC-DoxR and BC cells but could not affect STAT3 mRNA expression, indicating that FTO was not involved in the m6A modification of STAT3. However, FTO could activate STAT3 signaling in BC-DoxR and BC cells. Besides, FTO knockdown inhibited the doxorubicin resistance of BC-DoxR cells, while FTO overexpression enhanced the doxorubicin resistance and weakened the doxorubicin sensitivity of BC cells. Moreover, decreased doxorubicin resistance by STAT3 knockdown was abolished by FTO overexpression and decreased doxorubicin sensitivity by STAT3 overexpression was reversed by FTO knockdown, indicating that FTO was implicated in STAT3-mediated doxorubicin resistance and impairment of doxorubicin sensitivity of BC cells. Altogether, our findings provide a mechanism underlying BC doxorubicin resistance.
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http://dx.doi.org/10.1080/21655979.2021.1924544DOI Listing
December 2021

P300/HDAC1 regulates the acetylation/deacetylation and autophagic activities of LC3/Atg8-PE ubiquitin-like system.

Cell Death Discov 2021 May 31;7(1):128. Epub 2021 May 31.

Guangdong Laboratory for Lingnan Modern Agriculture/Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, 510642, Guangzhou, China.

Protein acetylation plays potential roles in regulating autophagy occurrence. However, it varies greatly between yeast and mammals, and has not been thoroughly investigated in other organisms. Here, we reported that the components of BmAtg8-PE ubiquitin-like system (BmAtg3, BmAtg4, BmAtg7, and BmAtg8) in Bombyx mori were localized in the nucleus under nutrient-rich conditions, whereas they were exported to the cytoplasm upon autophagy induction. RNAi of BmP300 and inhibition of BmP300 activity resulted in nucleo-cytoplasmic translocation of BmAtg3 and BmAtg8, as well as premature induction of autophagy in the absence of stimulus. Conversely, RNAi of BmHDAC1 and inhibition of class I/II HADCs activities led to the nuclear accumulation of BmAtg3 and BmAtg8. In addition, acetylation sites in Atg proteins of BmAtg8-PE ubiquitin-like system were identified by mass spectrometry, and acetylation-site mutations caused nucleo-cytoplasmic translocation of BmAtg3, BmAtg4, and BmAtg8 along with autophagy promotion. Similarly, the subcellular localization of human ATG4b is determined by acetylation modification. In general, BmP300-mediated acetylation sequesters the components of BmAtg8-PE ubiquitin-like system in the nucleus, thus leading to the autophagy inhibition. Oppositely, BmHDAC1-mediated deacetylation leads to the nucleo-cytoplasmic translocation of the components of BmAtg8-PE ubiquitin-like system and promotes autophagy. This process is evolutionarily conserved between insects and mammals.
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http://dx.doi.org/10.1038/s41420-021-00513-0DOI Listing
May 2021

Formation of Formic Acid from Glucose with Simultaneous Conversion of AgO to Ag under Mild Hydrothermal Conditions.

ACS Omega 2021 May 20;6(17):11260-11265. Epub 2021 Apr 20.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.

Formation of formic acid from renewable biomass resources is of great interest since formic acid is a widely used platform chemical and has recently been regarded as an important liquid hydrogen carrier. Herein, a novel approach is reported for the conversion of glucose, the constituent carbohydrate from the cellulose fraction of biomass, to formic acid under mild hydrothermal conditions with simultaneous reduction of AgO to Ag. Results showed that glucose was selectively converted to formic acid with an optimum yield of 40.7% and glycolic acid with a yield of 6.1% with 53.2% glucose converting to carbon dioxide (CO) immediately at a mild reaction temperature of 135 °C for 30 min. In addition, AgO was used as a solid oxidant for glucose oxidation, which avoids the use of traditionally dangerous liquid oxidant HO. Furthermore, complete conversion of AgO to Ag can be achieved. This study not only developed a new method for value-added chemical production from renewable biomass but also explored an alternative low-carbon and energy-saving route for silver extraction and recovery.
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http://dx.doi.org/10.1021/acsomega.1c00106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153907PMC
May 2021

Pulmonary stromal expansion and intra-alveolar coagulation are primary causes of COVID-19 death.

Heliyon 2021 May 24;7(5):e07134. Epub 2021 May 24.

Department of Pathology/Cytology, Karolinska University Laboratory, 141 86 Stockholm, Sweden.

Most COVID-19 victims are old and die from unrelated causes. Here we present welve complete autopsies, including two rapid autopsies of young patients where the cause of death was COVID-19 ARDS. The main virus induced pathology was in the lung parenchyma and not in the airways. Most coagulation events occurred in the intra-alveolar and not in the intra-vascular space and the few thrombi were mainly composed of aggregated thrombocytes. The dominant inflammatory response was the massive accumulation of CD163 + macrophages and the disappearance of T killer, NK and B-cells. The virus was replicating in the pneumocytes and macrophages but not in bronchial epithelium, endothelium, pericytes or stromal cells. The lung consolidations were produced by a massive regenerative response, stromal and epithelial proliferation and neovascularization. We suggest that thrombocyte aggregation inhibition, angiogenesis inhibition and general proliferation inhibition may have a roll in the treatment of advanced COVID-19 ARDS.
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http://dx.doi.org/10.1016/j.heliyon.2021.e07134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141733PMC
May 2021

Cost-effectiveness analysis of the integrated control strategy for schistosomiasis japonica in a lake region of China: a case study.

Infect Dis Poverty 2021 May 28;10(1):79. Epub 2021 May 28.

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research On Tropical Diseases, Shanghai, 200025, China.

Background: Schistosomiasis japonica remains an important public health concern due to its potential to cause severe outcomes and long-term sequelae. An integrated control strategy implemented in the Peoples' Republic of China has been shown to be effective to control or interrupt the transmission of schistosomiasis. The objective of this study is to estimate the disease burden of schistosomiasis and assess the cost-effectiveness of the integrated control strategy focused on different major interventions at three stages for schistosomiasis control in a lake setting, to provide reference for policy making or planning.

Methods: Annual cost data of schistosomiasis control during 2009-2019 were obtained from the control program implementers in Jiangling County, Hubei Province, China. Economic costs are provided in constant 2009 Chinese Yuan (CNY). Epidemiological data of schistosomiasis were collected from the Jiangling county station for schistosomiasis control. Disease burden of schistosomiasis was assessed by calculating years of life lost (YLLs) owing to premature death, years lived with disability (YLDs) and disability-adjusted life years (DALYs). DALYs were calculated as the sum of YLLs and YLDs. We then conducted a rudimentary cost-effectiveness analysis by determining the ratio by dividing the difference between the average cost of integrated control strategy at transmission control (2013-2016) or transmission interruption (2017-2019) and the average cost at stage of infection control (2009-2012) with the difference between the DALYs of schistosomiasis at different control stages. Descriptive statistics on the costs and DALYs were used in the analysis.

Results: The total economic costs for schistosomiasis control in Jiangling County from 2009 to 2019 were approximately CNY 606.88 million. The average annual economic costs for schistosomiasis prevention and control at stages of infection control (2009-2012), transmission control (2013-2016), and transmission interruption (2017-2019) were approximately CNY 41.98 million, CNY 90.19 million and CNY 26.06 million respectively. The overall disease burden caused by schistosomiasis presented a downward trend. Meanwhile, the disease burden of advanced cases showed an upward trend with the DALY increased from 943.72 to 1031.59 person-years. Most disease burden occurred in the age group over 45 years old (especially the elderly over 60 years old). Taking the infection control stage as the control, the incremental cost-effectiveness ratio of integrated control strategy was CNY 8505.5 per case averted, CNY 60 131.6 per DALY decreased at transmission control stage and CNY -2217.6 per case averted, CNY -18 116.0 per DALY decreased at transmission interruption stage.

Conclusions: The disease burden of schistosomiasis decreased significantly with the implementation of the integrated prevention and control strategy. Surveillance and management on elder population should be strengthened to decrease diseases burden. There remains a need for well-conducted studies that examine the long-term cost-effectiveness of the integrated control strategy for schistosomiasis. GRAPHIC ABSTARCT.
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http://dx.doi.org/10.1186/s40249-021-00863-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161988PMC
May 2021

Kaempferol alleviates corneal transplantation rejection by inhibiting NLRP3 inflammasome activation and macrophage M1 polarization via promoting autophagy.

Exp Eye Res 2021 May 25;208:108627. Epub 2021 May 25.

Department of Ophthalmology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

Corneal transplantation rejection remains a major threat to the success rate of high-risk patients. Given the many side effects presented by traditional immunosuppressants, there is an urgency to clarify the mechanism of corneal transplantation rejection and to identify new therapeutic targets. Kaempferol is a natural flavonoid that has been proven in various studies to possess anti-inflammatory, antioxidant, anticancer, and neuroprotective properties. However, the effect of Ka on corneal transplantation remains largely unexplored. To address this, both at the in vivo and in vitro levels, we established a model of corneal allograft transplantation in Wistar rats and an LPS-induced inflammatory model using human THP-1-derived macrophages. In the transplantation experiments, we observed an enhancement of mRNA and protein level in the NLRP3/IL-1 β axis and in M1 macrophage polarization post-operation. In groups to which kaempferol intraperitoneal injections were administered, this response was effectively reduced. However, the effect of kaempferol was reversed after the application of autophagy inhibitors. Similarly, in the inflammatory model, we found that different concentrations of kaempferol reduced the LPS-induced M1 polarization and NLRP3 inflammasome activation. Moreover, we confirmed that kaempferol induced autophagy and that autophagy inhibitors reversed this effect in macrophages. In conclusion, we found that kaempferol can inhibit the activation of NLRP3 inflammasomes by inducing autophagy, thus inhibiting macrophage polarization, and ultimately alleviating corneal transplantation rejection. Thus, our study suggests that kaempferol is a potential therapeutic agent in the treatment of allograft rejection.
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http://dx.doi.org/10.1016/j.exer.2021.108627DOI Listing
May 2021

Transcriptional enhancement of GBP-5 by BATF aggravates sepsis-associated liver injury via NLRP3 inflammasome activation.

FASEB J 2021 Jun;35(6):e21672

General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, P.R. China.

Strong inflammatory response triggered by the activation of the innate immune system is one typical characteristic of sepsis-associated liver injury (SALI). Guanylate-binding protein 5 (GBP-5) is a component of cell-autonomous immunity and known to be associated with inflammation. Currently, whether GBP-5 participates in SALI and its roles in this disease are yet to be investigated. Using a lipopolysaccharide (LPS)-induced SALI mouse model, we found GBP-5 was highly expressed in LPS-treated mice, and its expression was tightly related to the serum concentrations of live injury markers and inflammatory cytokines, liver damage scores by H&E staining, and amounts of apoptotic hepatocytes by TUNEL staining. Moreover, GBP-5 overexpression was found to aggravate LPS-induced SALI by promoting the activation of NLR family pyrin domain containing 3 (NLRP3) inflammasome, then facilitated the production of pro-inflammatory cytokines, eventually induced hepatocyte cell death. Direct transcriptional activation of GBP-5 by basic leucine zipper ATF-like transcription factor (BATF) was identified and further validated. This study unveils a transcriptional upregulation of GBP-5 by interacting with BATF, which promotes the progression of LPS-induced SALI through NLRP3 inflammasome activation, and provides novel therapeutic insights for halting the progression of liver injury in various liver diseases.
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http://dx.doi.org/10.1096/fj.202100234RDOI Listing
June 2021

Covid-19 Impacts on Florida's Healthcare Professionals.

Hosp Top 2021 May 24:1-12. Epub 2021 May 24.

Brooks Rehabilitation, Jacksonville, FL, USA.

This study described how COVID-19 impacted employment, telehealth usage, and interprofessional collaboration. A cross-sectional survey was deployed in June 2020 to healthcare professionals in Florida. Job status was uniquely separated by profession, with more nurses and medical doctors reported having no effect, and more mental health counselors transitioned to telehealth. Over a third of rehabilitation providers reported being furloughed. Over forty percent of providers had no training in telehealth, yet 33.1% reported an increase in usage. Interprofessional interactions are lower across professions during the pandemic, compared with before. This study shows the need for additional training on telehealth and interprofessional collaboration.
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http://dx.doi.org/10.1080/00185868.2021.1927277DOI Listing
May 2021

Cerebral and myocardial mitochondrial injury differ in a rat model of cardiac arrest and cardiopulmonary resuscitation.

Biomed Pharmacother 2021 May 18;140:111743. Epub 2021 May 18.

Weil Institute of Emergency and Critical Care Research, Virginia Commonwealth University, Richmond, VA, USA; Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA; Department of Emergency Medicine, Virginia Commonwealth University Health System, Richmond, VA, USA. Electronic address:

Brain mitochondria are more sensitive to global ischemia compared to heart mitochondria. Complex I in the electron transport chain (ETC) is sensitive to ischemic injury and is a major control point of the rate of ADP stimulated oxygen consumption. The purpose of this study was to explore whether changes in cerebral and myocardial mitochondria differ after cardiac arrest. Animals were randomized into 4 groups (n = 6): 1) Sham 2) VF 3) VF+CPR 4) ROSC 1hr. Ventricular Fibrillation (VF) was induced through a guide wire advanced from the right jugular vein into the ventricle and untreated for 8 min. Resuscitation was attempted with a 4J defibrillation after 8 min of cardiopulmonary resuscitation (CPR). Brain mitochondria and cardiac mitochondrial subpopulations were isolated. Calcium retention capacity was measured to assess susceptibility to mitochondrial permeability transition pore opening. ADP stimulated oxygen consumption and ETC activity assays were performed. Brain mitochondria are far more sensitive to injury during cardiac arrest and resuscitation compared to cardiac mitochondria. Complex I is highly sensitive to injury in brain mitochondria. With markedly decreased calcium retention capacity, mitochondria contribute to cerebral reperfusion injury. Therapeutic preservation of cerebral mitochondrial activity and mitochondrial function during cardiac arrest may improve post-resuscitation neurologic function.
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http://dx.doi.org/10.1016/j.biopha.2021.111743DOI Listing
May 2021

Strengthening arsenite oxidation in water using metal-free ultrasonic activation of sulfite.

Chemosphere 2021 May 13;281:130860. Epub 2021 May 13.

Faculty of Material Science and Chemistry, China University of Geosciences, Wuhan, 430074, PR China. Electronic address:

Although sulfite-based advanced oxidation processes (AOPs) have received renewed attention due to the production of oxysulfur radicals, the feasibility of using ultrasound (US) to activate sulfite remains unknown. In this work, low frequency ultrasound has been applied for the first time to develop a novel sulfite activation process (US-S(IV)) for enhanced oxidation of arsenite (As(III)). Our results showed that the US-S(IV) process with 1 mM sulfite addition and 20 kHz 650 W ultrasound can achieve approximately 2.9-fold increase in As(III) oxidation rate compared to the US process at pH 7. The mechanisms underpinning the US-S(IV) process have been probed through radical-scavenging experiments and electron spin resonance (ESR) spectrometry. Direct ultrasonolysis of sulfite has been demonstrated to be the predominant pathway producing the primary sulfite radical (SO⁻) in the US-S(IV) process. Besides, the US-S(IV) process also works well in the treatment process of natural water, suggesting that this process could be promising in commercial scale application. This work not only provides a new application of ultrasound in sulfite-based AOP, but also provides further insights into how sulfite impacts the US process.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130860DOI Listing
May 2021

Rapid BMI Increases and Persistent Obesity in Small-for-Gestational-Age Infants.

Front Pediatr 2021 4;9:625853. Epub 2021 May 4.

Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

In order to compensate for the early intrauterine growth restriction, small-for-gestational age (SGA) infants have "catch-up growth" after birth. Increased caloric intake has been suggested for SGA infants conventionally. It is important to determine if the early growth rate of body mass index (BMI) is associated with risk of persistent obesity later in life. In this longitudinal cohort study, we assessed the BMI of a large cohort of children who were SGA at birth to determine their risk of persistent obesity at school age (6-7 years) due to excessive weight gain in the first 3 years of life. We collected the height and weight data of 23,871 SGA babies. A polynomial function was used to fit the BMI-for-age z-score (BAZ) values of 0-6 years old SGA children and interpolate their growth trajectory. In addition, we screened out 6,959 children from 23,871 children to further evaluate the dynamic changes of early childhood BMI. We divided the school-age children into groups as non-obese (BAZ < 2) and obese (BAZ > 2), and determined the association between changes in BMI and school-age obesity. From the perspective of BMI distribution, the interpolated growth trajectory indicated that SGA children reaching overweight status or developing obesity by 3 years of age, continued to have obesity until school age (R, 0.65; R, 0.21). The retrospective analysis showed that children who were overweight and had obesity during school age had a high BMI from early age. By analyzing the changes in early BMI, we found that the fastest growth of SGA children occurred in the early infancy before 6 months and they continued to grow rapidly for a period of time. Interestingly, former SGA children who maintained a near overweight (1 < BAZ < 2) status before the age of 2 maintained an appropriate growth rate and usually did not develop obesity. A rapid increase in BMI during early infancy in former SGA newborns leads to a persistent risk of obesity. The energy intake of SGA infants should appropriately meet the infants' growth needs and early BMI changes should be closely monitored for an optimal integrated management.
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http://dx.doi.org/10.3389/fped.2021.625853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129028PMC
May 2021

The effect of PBL combined with comparative nursing rounds on the teaching of nursing for traumatology.

Am J Transl Res 2021 15;13(4):3618-3625. Epub 2021 Apr 15.

Department of Ophthalmology, Affiliated Hospital of Beihua University Jilin, Jilin Province, China.

Objective: To investigate the effect of problem-based learning (PBL) combined with comparative nursing rounds on the teaching of nursing for traumatology.

Methods: This prospective study was performed in 186 nursing student interns. According to a random number table, these interns were assigned to the control group (n=93) and the experimental group (n=93). In the control group, the interns received traditional teaching methods. Meanwhile, interns in the experimental group received PBL combined with comparative nursing rounds teaching. The level of teaching approval, excellent and good rate of theoretical knowledge, operational ability, medical record writing, and critical thinking disposition inventory-Chinese version scores were compared between the two groups.

Results: Teaching approval in learning initiative, problem solving ability, critical thinking, clinical work ability, independent information acquisition ability, and teamwork spirit in the experimental group were improved compared with the control group (all P<0.05). The excellent and good rate of theoretical knowledge, operational ability, and medical record writing in the experimental group were all higher than those in the control group (all P<0.05). Compared with the control group, critical thinking disposition inventory-Chinese version scores in all aspects in the experimental group after training were improved (all P<0.05).

Conclusion: The application of PBL combined comparative nursing rounds in the teaching of nursing for traumatology is beneficial for a significant improvement in the grasp of theoretical knowledge, operational ability, critical thinking ability, and teaching approval.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129407PMC
April 2021