Publications by authors named "Jing Xie"

1,220 Publications

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[Research Progress in Glucose Metabolism of Chondrocytes].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Nov;52(6):923-928

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Dental and Endodontic Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Chondrocytes have a limited supply of glucose and oxygen for metabolism since articular cartilages are relatively avascular. We herein reviewed the characteristics of chondrocyte glucose metabolism and the new research progress in chondrocyte glucose metabolism in the osteoarthritis process. Current research has shown that chondrocytes obtain glucose from synovial fluids and subchondral bones, take in glucose via specific glucose transporters, and metabolize glucose mainly through glycolysis and mitochondrial respiration to produce adenosine triphosphate (ATP). Glucose metabolism in chondrocytes is distinctive because it relies much more on glycolysis rather than mitochondrial respiration for ATP production, and shows Warburg effect and Crabtree effect. In osteoarthritic chondrocytes, the glucose metabolism disorder is presented as further suppression of mitochondrial respiration, over-active or impaired glycolysis, and decreased total production of ATP. However, the significance of the glucose supply for chondrocytes from synovial fluids and subchondral bones remains undefined. There are still disputes in the understanding of the changes in glycolytic pathways in osteoarthritic chondrocytes. Therefore, future research is needed to explore the characteristics of glucose metabolism in normal and osteoarthritic chondrocytes in order to develop new diagnostic and therapeutic strategies for osteoarthritis.
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http://dx.doi.org/10.12182/20211160206DOI Listing
November 2021

Factors That Affect the Sensitivity of Imaging Modalities in Primary Hyperparathyroidism.

Int J Endocrinol 2021 20;2021:3108395. Epub 2021 Feb 20.

Department of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Endocrine and Metabolic Diseases, and Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai 200025, China.

Background: Cervical ultrasound, Tc-sestamibi single-photon emission computed tomography/computed tomography (Tc-MIBI SPECT/CT), and cervical CT are routinely used in preoperative localization of primary hyperparathyroidism (PHPT). However, false-negative imaging results are also frequently encountered in clinical practice. Exploring the factors that affect the sensitivity of these imaging modalities is important for the surgical management of PHPT patients.

Methods: Clinical data of 352 PHPT patients hospitalized in our center from January 2011 to December 2015 were retrospectively collected to evaluate the sensitivity of 3 imaging modalities in the preoperative localization of parathyroid lesions. The ROC curve analysis was used to explore the clinical factors affecting the sensitivity of localization, and the cut-point(s) of related factors were determined.

Results: Tc-MIBI SPECT/CT has the highest sensitivity among the localization modalities commonly used, reaching 91.1% (86.0%-94.8%). When the lengths of parathyroid lesions were ≤1.3 cm, the sensitivity of neck ultrasonography significantly decreased, while the sensitivity of Tc-MIBI SPECT/CT decreased with parathyroid lesions ≤1.3 cm or serum PTH≤252 pg/ml. Tc-MIBI SPECT/CT was less effective in localizing the hyperplasia lesions. Neck ultrasonography combined with Tc-MIBI SPECT/CT can effectively improve the accuracy of preoperative localization of parathyroid lesions to 96.2% (92.7%-98.1%).

Conclusions: Small parathyroid lesion and mild elevation of serum PTH would reduce the accuracy of parathyroid localization in PHPT patients.
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http://dx.doi.org/10.1155/2021/3108395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616673PMC
February 2021

Effects of Edible Coating and Modified Atmosphere Technology on the Physiology and Quality of Mangoes after Low-Temperature Transportation at 13 °C in Vibration Mitigation Packaging.

Plants (Basel) 2021 Nov 11;10(11). Epub 2021 Nov 11.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

The mango is an important tropical fruit in the world, but it is easily perishable after harvest. In order to investigate the effect of the compound preservation technology on the physiology and quality of mangoes during transportation and storage, mangoes were treated with different packaging and preservation methods. All mangoes were subjected to simulated transportation by a vibration table for 24 h (180 r/min, 13 °C), and stored at 13 °C. The changes in the color, physicochemical characteristics, quality, and antioxidant-related enzymes of the mangoes were measured. The results show that the shelf life of inflatable bag packing (CK) was only 24 d, while the other treatments could be 30 d. The inflatable bag packing with modified atmosphere packaging (MAP) treatment (HPM) had the lowest yellowing degree (12.5%), disease index (34.4%), and mass loss (2.95%), at 30 d. Compared with the CK, the compound treatment containing MAP prolonged the peak respiration of the mangoes by 6 d and suppressed the increase in the total soluble solids and relative conductivity. Meanwhile, the HPM could effectively maintain moisture content, firmness, titratable acid, vitamin C, and the peroxidase and superoxide dismutase content, indicating that the treatment could maintain the better quality and antioxidation ability of mangoes. In summary, the MAP compound treatment better maintained the commercial characteristics of the mangoes, followed by the edible coating compound treatment. The results provide a theoretical reference for mango cushioning packaging and postharvest storage technology.
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http://dx.doi.org/10.3390/plants10112432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621718PMC
November 2021

Effects of Freeze-Thaw Cycles on Water Migration, Microstructure and Protein Oxidation in Cuttlefish.

Authors:
Ying Lv Jing Xie

Foods 2021 Oct 26;10(11). Epub 2021 Oct 26.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

This study was conducted to analyze the effects of multiple freeze-thaw (F-T) cycles on microstructural disruption, water migration, protein oxidation and textural properties of cuttlefish. Low-field nuclear magnetic resonance (LF-NMR) showed an increase in the proportion of free water in cuttlefish flesh. It was also observed by scanning electron microscopy (SEM) that multiple F-T cycles increased the gap between muscle fibers and disrupted the original intact and compact structure. The results of Fourier transform infrared spectroscopy, intrinsic fluorescence spectroscopy, CaATPase content, sulfhydryl content and free amino acid content indirectly prove that multiple F-T cycles can lead to the destruction of the a-helical structure of cuttlefish myofibril protein and the content of irregular curls increased, protein aggregation and degradation, and tryptophan oxidation. In addition, after repeated freezing and thawing, the water holding capacity, whiteness value, elasticity and chewiness of cuttlefish flesh decreased, the total volatile base nitrogen content increased. It can be concluded that the freeze-thaw cycles are very harmful to the quality of the frozen foods, so it is important to keep the temperature stable in the low-temperature food logistics.
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http://dx.doi.org/10.3390/foods10112576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620184PMC
October 2021

Toxic Effects on Bioaccumulation, Hematological Parameters, Oxidative Stress, Immune Responses and Tissue Structure in Fish Exposed to Ammonia Nitrogen: A Review.

Animals (Basel) 2021 Nov 19;11(11). Epub 2021 Nov 19.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Ammonia nitrogen is the major oxygen-consuming pollutant in aquatic environments. Exposure to ammonia nitrogen in the aquatic environment can lead to bioaccumulation in fish, and the ammonia nitrogen concentration is the main determinant of accumulation. In most aquatic environments, fish are at the top of the food chain and are most vulnerable to the toxic effects of high levels of ammonia nitrogen exposure. In fish exposed to toxicants, ammonia-induced toxicity is mainly caused by bioaccumulation in certain tissues. Ammonia nitrogen absorbed in the fish enters the circulatory system and affects hematological properties. Ammonia nitrogen also breaks balance in antioxidant capacity and causes oxidative damage. In addition, ammonia nitrogen affects the immune response and causes neurotoxicity because of the physical and chemical toxicity. Thence, the purpose of this review was to investigate various toxic effects of ammonia nitrogen, including oxidative stress, neurotoxicity and immune response.
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http://dx.doi.org/10.3390/ani11113304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614401PMC
November 2021

Giant single-crystal-to-single-crystal transformations associated with chiral interconversion induced by elimination of chelating ligands.

Nat Commun 2021 Nov 25;12(1):6908. Epub 2021 Nov 25.

Key Laboratory of Cluster Science of Ministry of Education, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.

Numerous single crystals that exhibit single-crystal-to-single-crystal (SCSC) transformations have been reported, and some of them show great promise for application to advanced adsorption materials, magnetic switches, and smart actuators. However, the development of single crystals with super-adaptive crystal lattices capable of huge and reversible structural change remains a great challenge. In this study, we report a Zn complex that undergoes giant SCSC transformation induced by a two-step thermal elimination of ethylene glycol chelating ligands. Although the structural change is exceptionally large (50% volume shrinkage and 36% weight loss), the single-crystal nature of the complex persists because of the multiple strong hydrogen bonds between the constituent molecules. This allows the reversible zero-dimensional to one-dimension and further to three-dimensional structural changes to be fully characterized by single-crystal X-ray diffraction analyses. The elimination of chelating ligands induces a chiral interconversion in the molecules that manifests as a centric-chiral-polar symmetric variation of the single crystal. The study not only presents a unique material, featuring both a periodic crystal lattice and gel-like super-ductility, but also reveals a possible solid-state reaction method for preparing chiral compounds via the elimination of chelating ligands.
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http://dx.doi.org/10.1038/s41467-021-27282-9DOI Listing
November 2021

Hsa_circ_0000527 Downregulation Suppresses the Development of Retinoblastoma by Modulating the miR-27a-3p/HDAC9 Pathway.

Curr Eye Res 2021 Nov 25:1-12. Epub 2021 Nov 25.

Department of Ophthalmology, Xingtai People's Hospital, Xingtai, Hebei, China.

Background: Accumulating evidence indicates that the progression of retinoblastoma (RB) may involve circRNA dysfunction. We aimed to disclose the role of hsa_circ_0000527 and its potential functional mechanism in RB.

Methods: The expression of hsa_circ_0000527, miR-27a-3p and histone deacetylase 9 (HDAC9) mRNA was monitored using quantitative real-time polymerase chain reaction (qPCR). Functional assays, including cell proliferation and apoptosis, were investigated using cell counting kit-8 (CCK-8) assay, colony formation assay and flow cytometry assay. The expression of apoptosis-associated proteins and HDAC9 protein was detected by western blot. The targeting relationship between miR-27a-3p and hsa_circ_0000527 or HDAC9 was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Besides, Xenograft models were constructed to confirm the effect of hsa_circ_0000527 .

Results: Hsa_circ_0000527 and HDAC9 were upregulated, while miR-27a-3p was downregulated in RB tissues and cells. Hsa_circ_0000527 downregulation repressed RB cell proliferation and induced RB cell apoptosis. MiR-27a-3p was a target of hsa_circ_0000527, and hsa_circ_0000527 suppressed the expression of miR-27a-3p. MiR-27a-3p inhibition reversed the role of hsa_circ_0000527 downregulation. In addition, HDAC9 was a target of miR-27a-3p, and hsa_circ_0000527 indirectly regulated HDAC9 expression by targeting miR-27a-3p. MiR-27a-3p restoration inhibited RB cell proliferation and promoted apoptosis, which was reversed by HDAC9 overexpression. Hsa_circ_0000527 downregulation could inactivate the PI3K/AKT pathway. Moreover, hsa_circ_0000527 downregulation blocked tumor growth rate .

Conclusion: hsa_circ_0000527 downregulation blocked the progression of RB by regulating the miR-27a-3p/HDAC9 pathway, which might be associated with the inactivation of the PI3K/AKT pathway.
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http://dx.doi.org/10.1080/02713683.2021.1925697DOI Listing
November 2021

The quality properties of frozen large yellow croaker fillets during temperature fluctuation cycles: improvement by cellobiose and carboxylated cellulose nanofibers.

Int J Biol Macromol 2021 Nov 23. Epub 2021 Nov 23.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China; Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China; Collaborative Innovation Center of Seafood Deep Processing, Ministry of Education, Dalian 116034, China. Electronic address:

Frozen aquatic products undergo unavoidable quality changes owing to temperature fluctuations during frozen storage and distribution. This study investigated the effects of 1% cellobiose (CB), and 0.5 and 1% carboxylated cellulose nanofibers (CNF) on ice crystal growth and recrystallization of frozen large yellow croaker fillets exposed to temperature fluctuations. Denser and more uniformly distributed ice crystals were observed in the CB- and CNF-treated samples than in the water-treated samples. Furthermore, the addition of CB and CNF suppressed the conversion of bound water to frozen water in the samples during temperature fluctuation cycles, played a positive role in fixing the ionic and hydrogen bonds that stabilize the protein structure, limited the conformational transition from α-helix to β-sheet, and improved protein thermal stability. Based on turbidity, zeta potential, and confocal laser scanning microscopy (CLSM) analyses, the presence of CB and CNF restricted the protein aggregation. Compared with CB, CNF molecules with abundant carboxyl functional groups and longer morphology exhibited better cryoprotective effects. Moreover, the fillets were more improved protected from mechanical damage induced by large ice crystals at a higher CNF concentration. This study reveals the potential of CB and CNF as novel cryoprotectants.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.11.093DOI Listing
November 2021

The Efficacy of Acupuncture on Tic Disorders in Children: A Retrospective and Propensity Score-Matched Study.

Front Pediatr 2021 4;9:745212. Epub 2021 Nov 4.

Department of Traditional Chinese Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Acupuncture has been considered as a complementary or alternative therapy for children with tic disorders (TD), but its efficacy remains largely unknown. This study retrospectively examined the efficacy of acupuncture treatment for TD in children over the course of 12 weeks. Data were collected from Traditional Chinese Medicine clinics in a public pediatric hospital in Shanghai between June 2020 and March 2021. A total of 250 patients with TD were included in the study, with 122 patients exposed to acupuncture therapy combined with conventional treatment (observation group), and 128 patients exposed to conventional treatment alone (control group). Propensity score matching analyses were used to balance baseline characteristics, resulting in 78 matched patients for each group. Reductions in the Yale Global Tic Severity Scale (YGTSS) total score were analyzed in the two groups after 12 weeks of treatment. The two groups reached equilibrium in terms of baseline demographic characteristics and YGTSS total score after the propensity score matching ( > 0.05). Compared to the control group, the reduction in the YGTSS total score after 12 weeks of treatment was greater for the observation group (OR = 2.94, 95% CI: 1.03, 8.39, = 0.04), and this association was stronger for patients who had significant vocal tics (β = 0.29, 95% CI: 0.88, 2.68, = 0.001). The clinical efficacy for the observation group was significantly better than the control group. We provided preliminary evidence supporting the therapeutic effect of acupuncture for TD in children. Hence, our findings indicate that acupuncture could be an adjuvant treatment efficacious for TD in children, especially for vocal tics.
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http://dx.doi.org/10.3389/fped.2021.745212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600324PMC
November 2021

Long-term Patterns of Failure and the Value of Blood Prognostic Markers in Anal Cancers Treated With Intensity-Modulated Radiation Therapy.

Clin Colorectal Cancer 2021 Oct 23. Epub 2021 Oct 23.

Department of Radiation Oncology, Peter MacCallum Cancer Centre, Melbourne, Australia; Sir PeterMacCallum Department of Oncology, University of Melbourne, Parkville, Australia.

Background: To analyze the long-term outcomes and prognostic value of hematological parameters in anal cancer patients receiving intensity-modulated radiation therapy (IMRT).

Materials: Hospital records of consecutive patients with anal squamous cell carcinoma who received curative-intent IMRT according to a standardized contouring protocol between 2010 and 2020 were reviewed. Locoregional failure-free survival (LRFS), distant metastasis-free survival (DMFS), progression-free survival (PFS), and overall survival (OS) were estimated using the Kaplan-Meier method. Coverage of locoregional recurrences by the initial IMRT volumes were assessed. The prognostic value of pretreatment blood counts for PFS and OS were determined using Cox regression analysis.

Results: A total of 166 patients were analyzed with a median follow-up of 3.3 years. Forty-six percent and 54% of patients had Stage I-II and IIIA-B cancers, respectively. The 5-year LRFS, DMFS, PFS and OS were 81%, 89%, 65% and 76% respectively. Grade ≥ 3 toxicity occurred in 5% of patients. Of all patients who relapsed, 70% had only locoregional recurrence as first site of failure. Ninety percent of locoregional recurrences were in-field. Hemoglobin, neutrophil and platelet counts were associated with PFS on univariable analysis, but only cancer stage and p16 status remained prognostic on multivariable analysis. Patients with more advanced cancer stages also had higher baseline neutrophil counts. Performance status and neutrophil counts were prognostic for OS on multivariable analysis.

Conclusion: This study affirms the long-term efficacy and safety of IMRT. Treatment resistance, rather than radiation geographic miss, is a major issue underpinning locoregional recurrences. Pretreatment blood counts were not validated to be independently prognostic for disease recurrence.
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http://dx.doi.org/10.1016/j.clcc.2021.10.005DOI Listing
October 2021

Effects of chitosan and apple polyphenols coating on quality and microbial composition of large yellow croaker (Pseudosciaena crocea) during ice storage.

J Sci Food Agric 2021 Nov 15. Epub 2021 Nov 15.

College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.

Background: Large yellow croaker (Pseudosciaena crocea) has important commercial value because of its high nutritional value and delicious taste. However, large yellow croaker is readily affected by microorganisms during storage, which causes the corruption of muscle tissue. Both chitosan and apple polyphenols are bio-preservatives, which can effectively inhibit the growth of microorganisms and improve the quality of large yellow croaker. The effects of 10.0 g/L and 20.0 g/L chitosan (CS) combined with 1.0 g/L apple polyphenols (AP) coating on the quality and microbial composition of large yellow croaker during ice storage were investigated respectively.

Results: CS+AP coating restrained the increase of TVB-N and biogenic amines, slowed down the rise of K-value and retarded the growth of microorganisms. The bacteriostatic effect was positively correlated with the concentration of CS. Through the analysis of high throughput sequencing (HTS), the microbial diversity was changed respectively. The proportion of Shewanella was significantly decreased by CS+AP coating treatment and Pseudomonas was the dominant microorganism in spoiled samples. Compared with the shelf-life of CK group (8 days), 20.0 g/L CS combined with 1.0 g/L AP coating treatment could extend the shelf-life of large yellow croaker for another 8 days.

Conclusions: CS combined with AP coating may be considered a promising method to delay the biochemical changes of ice stored large yellow croaker and extend its shelf life. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jsfa.11651DOI Listing
November 2021

Comparison of Physicochemical Changes and Water Migration of , , and Cocultures From Spoiled Bigeye Tuna () During Cold Storage.

Front Microbiol 2021 29;12:727333. Epub 2021 Oct 29.

Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, China.

This study investigates the physicochemical changes and water migration of (A), (S), and cocultured and (AS) inoculated into bigeye tuna during cold storage. The physicochemical indexes [fluorescence ratio (FR), total volatile base nitrogen (TVB-N), thiobarbituric acid (TBA), trimethylamine (TMA), peroxide value (POV), and pH] of bigeye tuna increased cold storage. A significant decrease in trapped water was found in the AS samples, and direct monitoring of the water dynamics was provided by low-field nuclear magnetic resonance. Samples inoculated with and also induced the degradation of myofibrillar proteins and weakness of some Z-lines and M-lines. Higher values of physicochemical indexes and water dynamics were shown in the coculture of and than in the other groups. Therefore, this paper reveals that the coculture of and resulted in a bigeye tuna that was more easily spoiled when compared to the single culture. This study provides insight into the spoilage potential of and during cold storage, which further assists in the application of appropriate technologies to keep the freshness of aquatic foods.
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http://dx.doi.org/10.3389/fmicb.2021.727333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586447PMC
October 2021

The deubiquitinase OTUB1 fosters papillary thyroid carcinoma growth through EYA1 stabilization.

J Cell Mol Med 2021 Nov 12. Epub 2021 Nov 12.

Department of Thyroid Surgery, Second Affiliated Hospital of Nanchang University, Nanchang, China.

Deubiquitinating enzyme OTU domain-containing ubiquitin aldehyde-binding proteins 1 (OTUB1) has been shown to have an essential role in multiple carcinomas. However, the function of OTUB1 in papillary thyroid cancer (PTC) and the underlying mechanisms regulating PTC cells proliferation remain poorly understood. In this study, OTUB1 was significantly upregulated in papillary thyroid carcinoma tissues and cells. Through in vitro and in vivo experiments, knockdown of OTUB1 suppressed PTC cells growth whereas OTUB1 overexpression enhanced the proliferation ability of PTC cells. Moreover, the eyes absent homologue 1 (EYA1) was recognized as a potential target of OTUB1 through mass spectrometry analysis, and we further verified that EYA1 protein level was positively correlated with OTUB1 expression in PTC cells and clinical samples. Mechanistically, OTUB1 could interact with EYA1 directly and deubiquitinate EYA1 to stabilize it. At last, EYA1 was found to play an essential role in OTUB1-derived PTC cells growth. Overall, our investigation reveals that OTUB1 is a previously unrecognized oncogenic factor in PTC cells proliferation and suggests that OTUB1 might be a novel therapeutic target in PTC.
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http://dx.doi.org/10.1111/jcmm.17020DOI Listing
November 2021

Deubiquitinase ZRANB1 drives hepatocellular carcinoma progression through SP1-LOXL2 axis.

Am J Cancer Res 2021 15;11(10):4807-4825. Epub 2021 Oct 15.

Department of Thyroid Surgery, Second Affiliated Hospital of Nanchang University Nanchang, Jiangxi, China.

Deubiquitinase (DUB) zinc finger RANBP2-type containing 1 (ZRANB1) has been reported to have a close relationship with cancers. However, its underlying role and molecular mechanisms in hepatocellular carcinoma (HCC) remain elusive. In this study, we demonstrated that ZRANB1 was highly expressed in HCC tissues. Additionally, ZRANB1 overexpression was correlated with poorer survival and ZRANB1 could be an independent predictor of poor prognosis for HCC patients. Through gain- and loss-of-function assays, we examined the oncogenic role of ZRANB1 in regulating HCC cell growth and metastasis and . To identify the downstream targets of ZRANB1 in regulating HCC tumorigenesis, we performed RNA-seq and demonstrated that Lysyl oxidase-like 2 (LOXL2) was the most significantly downregulated gene after ZRANB1 knockdown. Furthermore, the scatter plots indicated a significant positive correlation between ZRANB1 and LOXL2 expression in clinical HCC specimens. We also demonstrated that ZRANB1 knockdown downregulated the expression of LOXL2 and suppressed HCC growth and metastasis and . The effects of ZRANB1 knockdown were reversed by LOXL2 overexpression. More importantly, ZRANB1 regulated LOXL2 through specificity protein 1 (SP1) and SP1 overexpression rescued the suppression of HCC growth and metastasis induced by ZRANB1 knockdown. Mechanistically, ZRANB1 bound with SP1 directly and stabilized the SP1 protein by deubiquitinating it. The expression patterns of ZRANB1, SP1 and LOXL2 were evaluated in HCC patients. In summary, our research highlights a novel role of ZRANB1 in the tumorigenesis of HCC and suggests a new candidate prognostic biomarker for HCC treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569347PMC
October 2021

Effect of cellobiose on the myofibrillar protein denaturation induced by pH changes during freeze-thaw cycles.

Food Chem 2021 Nov 1:131511. Epub 2021 Nov 1.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquatic Product Processing&Preservation, Shanghai 201306, China; Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China. Electronic address:

The aim of this study was to investigate myofibrillar protein (MFP) denaturation induced by pH changes during freeze-thaw (FT) cycles, and to propose an effective mitigation strategy. Owing to the selective crystallization of NaHPO·12HO and the consequent pH change, a pH change of 3.32 units was observed when the MFP solution were frozen. The surface hydrophobicity, particle size and confocal laser scanning microscopy showed that the protein molecules gradually unfolded and formed larger protein aggregation as the number of FT cycles increases. Additionally, protein degradation, secondary and tertiary structure alterations suggested that the FT cycle could disrupt structural integrity. The addition of cellobiose could maximize the inhibition of pH changes (decrease of ∼0.62 unit), no NaHPO·12HO crystallization was observed by X-ray diffraction. Cellobiose could minimize FT damage to myofibrillar protein, which was closest to the control. Thus, cellobiose can be used as a new and effective cryoprotectant.
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http://dx.doi.org/10.1016/j.foodchem.2021.131511DOI Listing
November 2021

Association between remnant cholesterol and arterial stiffness: A secondary analysis based on a cross-sectional study.

J Clin Hypertens (Greenwich) 2021 Nov 9. Epub 2021 Nov 9.

Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.

The relationship between conventional lipid parameters and arterial stiffness (AS) has been verified by previous studies. However, it remains unknown whether non-conventional lipid parameters have certain predictive effect on AS represented by brachial-ankle pulse wave velocity (baPWV). Therefore, the study was to explore the relationship between remnant cholesterol (RC) and other non-conventional lipid parameters and AS in the general population free from cardiovascular disease. The study included 912 participants aged 24-84 years from a medical health checkup center of Murakami Memorial Hospital. Logistic regression analysis and receiver operating characteristic (ROC) curves were used to examine the association between non-conventional lipid parameters and AS. The results showed that compared with non-AS group, the AS group had higher RC, non-high-density lipoprotein cholesterol (Non-HDL-C), atherogenic index of plasma (AIP), lipoprotein combine index (LCI), atherosclerosis index (AI), triglycerides/HDL-C (TG/HDL-C), Castelli's risk index I (CRI-I) and Castelli's risk index II (CRI-II). Then, the authors divided participants into two groups by the optimal cutoff point of 23.6 of RC determined by Youden index. The baPWV was significantly higher in higher RC group compared with lower RC group, and RC was positively correlated with baPWV. Multivariate Logistic regression analysis showed that, regarding lower RC as reference, higher RC was independently associated with higher risk of AS, independent of other risk factors (OR = 1.794, 95% CI: 1.267-2.539, p = .001). The area under the curve of AS predicted by RC was higher than that of other non-conventional lipid parameters (almost all p < .05). The findings indicated that increased RC was a significant predictor of AS.
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http://dx.doi.org/10.1111/jch.14384DOI Listing
November 2021

In Situ Replacement Synthesis of [email protected] Encapsulated CoPt @Co P Heterojunction Boosting Methanol Oxidation and Hydrogen Evolution.

Small 2021 Nov 5:e2104656. Epub 2021 Nov 5.

State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources, Key Laboratory of Advanced Functional Materials, Autonomous Region, Institute of Applied Chemistry, College of Chemistry, Xinjiang University, Urumqi, Xinjiang, 830046, P. R. China.

Simultaneous boosting electrochemical methanol oxidation reaction (MOR) for direct methanol fuel cells and production of hydrogen is meaningful but challenging. Herein, a sea urchin-shaped cobalt-embedded N-doped carbon nanotubes ([email protected]) encapsulated CoPt @Co P heterojunction (CoPt @Co P/[email protected]) is fabricated. Theoretical calculations confirm that electrons at the interfaces transfer from CoPt to Co P, where electron hole region on CoPt is beneficial to improving the MOR activity, whereas accumulation region on Co P favors to the optimization of H O and H* absorption energies for hydrogen evolution reaction (HER). Benefitting from its interfacial electronic reconfiguration, the CoPt @Co P/[email protected] heterojunction exhibits excellent electrocatalytic performances for MOR and HER, in which the mass activity (2981 mA mg ) for MOR is 14.2 times than that of Pt/C (20%), and the smallest overpotentials only requires 19 mV to deliver a current density of 10 mA cm for HER. Moreover, the electrolyzer employing CoPt @Co P/[email protected] for anodic MOR and cathodic H production only requires a low voltage of 1.43 V at 10 mA cm with impressive long-life cycling stability, which is obviously better than that of commercial Pt/C//RuO . This study offers a novel strategy for other organics oxidation reaction coupled with HER catalyzed production of hydrogen.
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http://dx.doi.org/10.1002/smll.202104656DOI Listing
November 2021

Preparation of chitosan grafted caffeic acid coating and its effect on pompano (Trachinotus ovatus) preservation.

J Sci Food Agric 2021 Nov 5. Epub 2021 Nov 5.

College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.

Background: The present study aimed to investigate the preservative effect of chitosan-caffeic acid grafts coating (CS-g-CA) on the quality and microbial characteristics of pompano (Trachinotus ovatus) during iced storage. CS-g-CA was prepared by a 1-(3-dimethylaminopropyl)-3-ethylcarbodiimidehydro/N-hydroxysuccinimide coupling reaction. The grafting of CS-g-CA was confirmed by UV-visible and Fourier-transform infrared spectra. Samples were treated with distilled water (control), chitosan (CS), caffeic acid (CA) and CS-g-CA for 10 min, respectively. Microbiological [total viable count (TVC), H S-producing bacteria count, Pseudomonas bacteria count], physicochemical indicators [water holding capacity (WHC), cooking loss, pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), texture profile analysis, free amino acids] and sensory evaluation were investigated during ice storage at 4 °C for up to 27 days.

Results: The results showed that the antioxidant and antibacterial activities of CS could be improved by grafting CA onto CS. CS-g-CA coating could greatly slow down the speed of water loss and maintain WHC. Furthermore, CS-g-CA coating showed superior antibacterial activities by inhibiting the growth of TVC, delayed the decline of flavor amino acids and reduced sensory change. In addition, CS-g-CA coating reduced lipid oxidation and protein degradation as indicated by the decrease in TBA and TVB-N, possibly as a result of the addition of CA into CS membrane significantly improving the antioxidant activity of CS.

Conclusion: Compared with the control group, CS-g-CA coating had the optimal effect and could enhance the shelf-life of Trachinotus ovatus for at least another 9 days. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11624DOI Listing
November 2021

Osteoblasts impair cholesterol synthesis in chondrocytes via Notch1 signalling.

Cell Prolif 2021 Nov 2:e13156. Epub 2021 Nov 2.

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Objectives: Previous reports have proposed the importance of signalling and material exchange between cartilage and subchondral bone. However, the specific experimental evidence is still insufficient to support the effect of this interdependent relationship on mutual cell behaviours. In this study, we aimed to investigate cellular lipid metabolism in chondrocytes induced by osteoblasts.

Methods: Osteoblast-induced chondrocytes were established in a Transwell chamber. A cholesterol detection kit was used to detect cholesterol contents. RNA sequencing and qPCR were performed to assess changes in mRNA expression. Western blot analysis was performed to detect protein expression. Immunofluorescence staining was conducted to show the cellular distribution of proteins.

Results: Cholesterol levels were significantly decreased in chondrocytes induced by osteoblasts. Osteoblasts reduced cholesterol synthesis in chondrocytes by reducing the expression of a series of synthetases, including Fdft1, Sqle, Lss, Cyp51, Msmo1, Nsdhl, Sc5d, Dhcr24 and Dhcr7. This modulatory process involves Notch1 signalling. The expression of ncstn and hey1, an activator and a specific downstream target of Notch signalling, respectively, were decreased in chondrocytes induced by osteoblasts.

Conclusions: For the first time, we elucidated that communication with osteoblasts reduces cholesterol synthesis in chondrocytes through Notch1 signalling. This result may provide a better understanding of the effect of subchondral bone signalling on chondrocytes.
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http://dx.doi.org/10.1111/cpr.13156DOI Listing
November 2021

The investigation of storage situation of fish muscle via the analysis of its exudate by MALDI-TOF MS.

Food Chem 2021 Oct 21;373(Pt A):131450. Epub 2021 Oct 21.

College of Food Science and Engineering, Shanghai Ocean University, Hucheng Ring Road 999, Pudong New District, Shanghai 201306, China. Electronic address:

The potential of MALDI-TOF MS was investigated in terms of its capability to determine the change of exudates of fish muscle with different freeze-thaw cycles (0, 1 and 2), and frozen storage periods. The exudates were collected from dead chilled marine fish species, large yellow croacker (Larimichthys crocea, LC) and freshly slaughtered freshwater fish species, Japanese seabass (Lateolabrax japonicus) to be studied as models. 109 proteins, in which, 32 are extracellular proteins, and 15 are intracellular proteins, were identified by analyzing exudate of LC using MALDI-TOF MS and HPLC-MS/MS. The results show that the present method may be able to determine the change of fish muscle foods in a more sensitive mode than K value indicated quality control. The feasibility of verifying the storage situation of fish sample was performed by analyzing fish samples obtained from the local market. It is promising to estimate the storage situation of fishery products or other animal muscle foods by analyzing their muscle exudates based on the presently developed strategy.
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http://dx.doi.org/10.1016/j.foodchem.2021.131450DOI Listing
October 2021

Short-Chain Fatty Acids Produced by Ruminococcaceae Mediate α-Linolenic Acid Promote Intestinal Stem Cells Proliferation.

Mol Nutr Food Res 2021 Oct 28:e2100408. Epub 2021 Oct 28.

Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, P. R. China.

Scope: The proliferation and differentiation of intestinal stem cells (ISCs) are the basis of intestinal renewal and regeneration, and gut microbiota plays an important role in it. Dietary nutrition has the effect of regulating the activity of ISCs; however, the regulation effect of α-linolenic acid (ALA) has seldom been reported.

Methods And Results: After intervening mice with different doses of ALA for 30 days, it is found that ALA (0.5 g kg ) promotes small intestinal and villus growth by activating the Wnt/β-catenin signaling pathway to stimulate the proliferation of ISCs. Furthermore, ALA administration increases the abundance of the Ruminococcaceae and Prevotellaceae, and promotes the production of short-chain fatty acids (SCFAs). Subsequent fecal transplantation and antibiotic experiments demonstrate that ALA on the proliferation of ISCs are gut microbiota dependent, among them, the functional microorganism may be derived from Ruminococcaceae. Administration of isobutyrate shows a similar effect to ALA in terms of promoting ISCs proliferation. Furthermore, ALA mitigates 5-fluorouracil-induced intestinal mucosal damage by promoting ISCs proliferation.

Conclusion: These results indicate that SCFAs produced by Ruminococcaceae mediate ALA promote ISCs proliferation by activating the Wnt/β-catenin signaling pathway, and suggest the possibility of ALA as a prebiotic agent for the prevention and treatment of intestinal mucositis.
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http://dx.doi.org/10.1002/mnfr.202100408DOI Listing
October 2021

Are Osteoclasts Mechanosensitive Cells?

J Biomed Nanotechnol 2021 Oct;17(10):1917-1938

State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610064, China.

Skeleton metabolism is a process in which osteoclasts constantly remove old bone and osteoblasts form new osteoid and induce mineralization; disruption of this balance may cause diseases. Osteoclasts play a key role in bone metabolism, as osteoclastogenesis marks the beginning of each bone remodeling cycle. As the only cell capable of bone resorption, osteoclasts are derived from the monocyte/macrophage hematopoietic precursors that terminally adhere to mineralized extracellular matrix, and they subsequently break down the extracellular compartment. Bone is generally considered the load-burdening tissue, bone homeostasis is critically affected by mechanical conductions, and the bone cells are mechanosensitive. The functions of various bone cells under mechanical forces such as chondrocytes and osteoblasts have been reported; however, the unique bone-resorbing osteoclasts are less studied. The oversuppression of osteoclasts in mechanical studies may be because of its complicated differentiation progress and flexible structure, which increases difficulty in targeting mechanical structures. This paper will focus on recent findings regarding osteoclasts and attempt to uncover proposed candidate mechanosensing structures in osteoclasts including podosome-associated complexes, gap junctions and transient receptor potential family (ion channels). We will additionally describe possible mechanotransduction signaling pathways including GTPase ras homologue family member A (RhoA), Yes-associated protein/transcriptional co-activator with PDZ-binding motif (TAZ), Ca signaling and non-canonical Wnt signaling. According to numerous studies, evaluating the possible influence of various physical environments on osteoclastogenesis is conducive to the study of bone homeostasis.
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http://dx.doi.org/10.1166/jbn.2021.3171DOI Listing
October 2021

Zwitterionic polymer modified xanthan gum with collagen II-binding capability for lubrication improvement and ROS scavenging.

Carbohydr Polym 2021 Nov 17;274:118672. Epub 2021 Sep 17.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, PR China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China; Med-X Center for Materials, Sichuan University, Chengdu 610041, PR China. Electronic address:

High friction of damaged cartilage requires long-acting lubricated additive, which can also effectively scavenge reactive oxidative species (ROS) produced by mechanically stimulated chondrocytes. In this study, xanthan gum (XG) was grafted by poly (sulfobetaine methacrylate) (PSBMA) (the [XG]/[SBMA] molar ratio is 1:5 or 1:10), forming nanoparticles and then conjugated with collagen II-binding peptide, finally obtaining CBPXGSB1/5 or CBPXGSB1/10. Therein, the CBPXGSB1/5 was chosen as optimal lubricated additive. The results show that hydrated effect of PSBMA side chains endows CBPXGSB1/5 with favorable lubrication property (COF is 0.063). Furthermore, the CBPXGSB1/5 combining lubrication property and specific binding capability together may achieve the long-acting lubrication for injured cartilage in medical field. The CBPXGSB1/5 also possesses antioxidation verified by DPPH assay and exhibits synergistically enhanced ROS (OH, O and HO) scavenging. Besides, cytotoxicity experiment demonstrates that CBPXGSB1/5 has good biocompatibility. Therefore, multifunctional CBPXGSB1/5 developed here may have promising application potential in osteoarthritis treatment.
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http://dx.doi.org/10.1016/j.carbpol.2021.118672DOI Listing
November 2021

Deep attention branch networks for skin lesion classification.

Comput Methods Programs Biomed 2021 Nov 2;212:106447. Epub 2021 Oct 2.

The Wenzhou Third Clinical Institute Affiliated To Wenzhou Medical University, Wenzhou, 325000, China; Wenzhou People's Hospital, Wenzhou, 325000, China. Electronic address:

Background And Objective: The skin lesion usually covers a small region of the dermoscopy image, and the lesions of different categories might own high similarities. Therefore, it is essential to design an elaborate network for accurate skin lesion classification, which can focus on semantically meaningful lesion parts. Although the Class Activation Mapping (CAM) shows good localization capability of highlighting the discriminative parts, it cannot be obtained in the forward propagation process.

Methods: We propose a Deep Attention Branch Network (DABN) model, which introduces the attention branches to expand the conventional Deep Convolutional Neural Networks (DCNN). The attention branch is designed to obtain the CAM in the training stage, which is then utilized as an attention map to make the network focus on discriminative parts of skin lesions. DABN is applicable to multiple DCNN structures and can be trained in an end-to-end manner. Moreover, a novel Entropy-guided Loss Weighting (ELW) strategy is designed to counter class imbalance influence in the skin lesion datasets.

Results: The proposed method achieves an Average Precision (AP) of 0.719 on the ISIC-2016 dataset and an average area under the ROC curve (AUC) of 0.922 on the ISIC-2017 dataset. Compared with other state-of-the-art methods, our method obtains better performance without external data and ensemble learning. Moreover, extensive experiments demonstrate that it can be applied to multi-class classification tasks and improves mean sensitivity by more than 2.6% in different DCNN structures.

Conclusions: The proposed method can adaptively focus on the discriminative regions of dermoscopy images and allows for effective training when facing class imbalance, leading to the performance improvement of skin lesion classification, which could also be applied to other clinical applications.
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http://dx.doi.org/10.1016/j.cmpb.2021.106447DOI Listing
November 2021

FGF7-induced E11 facilitates cell-cell communication through connexin43.

Int J Biol Sci 2021 3;17(14):3862-3874. Epub 2021 Sep 3.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.

Fibroblast growth factors (FGFs) include a large family of growth factors that play a critical role in maintaining bone homeostasis, but the specific role of its members such as FGF7 does not well understand. Osteoblasts are a kind of major cells essential for bone formation. Osteoblasts interact with one another to create the unique structure of osteons. The well-connected osteons constitute the cortical bone. As an early osteocyte marker that triggers actin cytoskeleton dynamics, E11 is essential for osteoblasts' dendrites formation. However, the upstream which regulates E11 is mainly unknown. The purpose of this study was to examine the influence of FGF7 on the expression and the distribution of E11 in osteoblasts, which mediated osteoblasts' processes formation and gap junctional intercellular communication (GJIC) partly through connexin43 (Cx43). We first demonstrated that FGF7 increased the expression of E11 in osteoblasts. We then showed that FGF7 promoted osteoblasts' dendrites elongation and functional gap junctions formation. Furthermore, E11 interacted directly with Cx43 in primary osteoblasts. MAPK pathway and PI3K-AKT pathway were involved in the effect of FGF7. Our results shed light on the unique role of FGF7 on osteoblasts, which may indicate that FGF7 plays a more significant role in the later stages of bone development and homeostasis.
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http://dx.doi.org/10.7150/ijbs.65240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495393PMC
September 2021

Metal-Nitrogen-Carbon Cluster-Decorated Titanium Carbide is a Durable and Inexpensive Oxygen Reduction Reaction Electrocatalyst.

ChemSusChem 2021 Nov 18;14(21):4613-4614. Epub 2021 Oct 18.

Department of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, Missouri, 63130, USA.

Invited for this month's cover are the groups of Prof. Vijay Ramani and Prof. Rohan Mishra at Washington University in St. Louis and their collaborators at Oak Ridge National Laboratory. The Full Paper itself is available at 10.1002/cssc.202101341.
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http://dx.doi.org/10.1002/cssc.202102054DOI Listing
November 2021

Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors.

Nat Commun 2021 10 15;12(1):6043. Epub 2021 Oct 15.

Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.

Clinical evidence has established that concomitant traumatic brain injury (TBI) accelerates bone healing, but the underlying mechanism is unclear. This study shows that after TBI, injured neurons, mainly those in the hippocampus, release osteogenic microRNA (miRNA)-enriched small extracellular vesicles (sEVs), which targeted osteoprogenitors in bone to stimulate bone formation. We show that miR-328a-3p and miR-150-5p, enriched in the sEVs after TBI, promote osteogenesis by directly targeting the 3'UTR of FOXO4 or CBL, respectively, and hydrogel carrying miR-328a-3p-containing sEVs efficiently repaires bone defects in rats. Importantly, increased fibronectin expression on sEVs surface contributes to targeting of osteoprogenitors in bone by TBI sEVs, thereby implying that modification of the sEVs surface fibronectin could be used in bone-targeted drug delivery. Together, our work unveils a role of central regulation in bone formation and a clear link between injured neurons and osteogenitors, both in animals and clinical settings.
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http://dx.doi.org/10.1038/s41467-021-26302-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519911PMC
October 2021

Bioelectric signaling and the control of cardiac cell identity in response to mechanical forces.

Science 2021 10 14;374(6565):351-354. Epub 2021 Oct 14.

Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR7104, Institut National de la Santé et de la Recherche Médicale U1258 and Université de Strasbourg, Illkirch, France.

[Figure: see text].
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http://dx.doi.org/10.1126/science.abc6229DOI Listing
October 2021

FDG-PET/CT imaging for evaluating durable responses to immune check point inhibitors in patients with advanced cutaneous squamous cell carcinoma.

Cancer Imaging 2021 Oct 13;21(1):57. Epub 2021 Oct 13.

Department of Medical Oncology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia.

Background: The role of FDG-PET/CT imaging in assessing response to immunotherapy in advanced cutaneous squamous cell carcinoma (CSCC) is unknown. This study compared complete metabolic response (CMR) rates by FDG-PET and RECIST1.1 via CT or MRI in patients on cemiplimab for > 10 months.

Methods: This was a single-centre retrospective study of 15 patients treated with cemiplimab for advanced CSCC who had CT/MRI and FDG-PET/CT at > 10 months to assess metabolic treatment response. The median age was 73 years (range 55-84) and 93% were male. RECIST1.1 and PERCIST1.0 tumor responses were evaluated by blinded readers.

Results: Seventy-three percent (11/15) (95%CI 44.9, 92.2%) achieved a CMR on PET. Of these 11, on RECIST1.1 there was one complete response, 9 partial responses and one stable disease.

Conclusions: In patients on cemiplimab for > 10 months, there was discordance between CR rates on FDG-PET versus RECIST1.1. FDG-PET/CT may have utility for clarifying depth of response in patients treated with immunotherapy for CSCC.
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http://dx.doi.org/10.1186/s40644-021-00426-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515684PMC
October 2021

Multi-frequency ultrasound:A potential method to improve the effects of surface decontamination and structural characteristics on large yellow croaker (Pseudosciaena crocea) during refrigerated storage.

Ultrason Sonochem 2021 Nov 8;79:105787. Epub 2021 Oct 8.

College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai 201306, China; National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), Shanghai 201306, China. Electronic address:

The effects of multi-frequency ultrasound on surface decontamination and structural characteristics of large yellow croaker (Pseudosciaena crocea) during refrigerated storage were evaluated. The results of total viable counts (TVCs) and psychrophilic bacteria counts (PBCs) demonstrated that multi-frequency ultrasound retarded the growth of microorganisms. The bacteriostatic effect was positively correlated with the increase of ultrasound frequencies. However, compared with triple-frequency ultrasound (TUS, 20/28/40 kHz) treatment, dual-frequency ultrasound (DUS, 20/28 kHz) treatment had higher water-holding capacity (WHC) and immobilized water content, better texture characteristics, lower pH and total volatile basic nitrogen (TVB-N). Through the results of myofibrillar fragmentation index (MFI), intrinsic fluorescence intensity (IFI) and atomic force microscope (AFM), multi-frequency ultrasound could effectively stabilize the myofibrillar protein structure of refrigerated large yellow croaker, which could maintain better texture characteristics. The effects of DUS were the most significant. Therefore, multi-frequency ultrasound treatment could inhibit the growth of microorganisms and improve the structural characteristics of large yellow croaker during refrigerated storage.
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http://dx.doi.org/10.1016/j.ultsonch.2021.105787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515294PMC
November 2021
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