Publications by authors named "Jing Wang"

13,715 Publications

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OsBSK2, a putative brassinosteroid-signaling kinase, positively controls grain size in rice.

J Exp Bot 2022 May 20. Epub 2022 May 20.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, Sichuan, China.

Grain size is an important trait that directly affects grain yield in rice; however, the genetic and molecular mechanisms regulating grain size remain unclear. In this study, we identified a mutant, grain length and grain weight 10 (glw10), which exhibited significantly reduced grain length and grain weight. Histological analysis demonstrated that GLW10 affects cell expansion, which regulates grain size. MutMap-based gene mapping and transgenic experiments demonstrated that GLW10 encodes a putative brassinosteroid (BR)-signaling kinase, OsBSK2. OsBSK2 is a plasma membrane protein, and an N-myristoylation site is needed for both its membrane localization and functions. OsBSK2 directly interacts with the BR receptor kinase OsBRI1, however, genetic experiments have demonstrated that OsBSK2 likely regulates grain size independently of the BR signaling pathway. OsBSK2 can form a homodimer or heterodimers with OsBSK3 and OsBSK4, and silencing OsBSK2, OsBSK3, and OsBSK4 reduced grain size. This indicates that OsBSKs seem to function as homodimers or heterodimers to positively regulate grain size in rice. They are both highly expressed in young panicles and spikelet hulls, which indicates that they regulate grain size. In summary, our results provide novel insights into the function of BSKs in rice and novel targets for improving grain size during crop breeding.
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http://dx.doi.org/10.1093/jxb/erac222DOI Listing
May 2022

Hydrogen sulfide supplement preserves mitochondrial function of retinal ganglion cell in a rat glaucoma model.

Cell Tissue Res 2022 May 20. Epub 2022 May 20.

Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China.

Glaucoma is a neurodegenerative disease of visual system characterized by gradual loss of retinal ganglion cells (RGC). Since mitochondrial dysfunction of RGC is significantly involved in the pathological mechanisms of glaucoma, and hydrogen sulfide (HS) takes part in the pathogeny of glaucoma and shows promising potential in restoring mitochondrial function in other neurons, the authors aimed to investigate the impact of HS on mitochondrial function of RGC with a rat glaucoma model. An established chronic ocular hypertension (COH) rat model induced by injection of cross-linking hydrogel into anterior chamber was adopted, and a HS donor, sodium hydrosulfide (NaHS), was selected to treat rats through intraperitoneal injection. After a period of 4 weeks, RGCs were isolated from the subjected rats with an immunopanning method and went through evaluations of mitochondrial membrane potential (MMP), mitochondrial permeability transition pore (MPTP) opening, intracellular Ca2 + level, reactive oxygen species (ROS) level, and cytosolic Cytochrome C distribution. The results showed that the mitochondrial function of RGC in experimental glaucoma was markedly improved by HS supplement, being presented as stabilization of MMP, alleviation of MPTP opening, improvement of intracellular Ca hemostasis, reduction of ROS accumulation, and inhibition of Cytochrome C release. Our study implicated that preservation of mitochondrial function by HS probably plays a key role in protecting RGC in the context of glaucomatous neuropathy, and it is worth further deepgoing research to benefit the development of glaucoma treatment.
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http://dx.doi.org/10.1007/s00441-022-03640-xDOI Listing
May 2022

Analgosedation in Critically Ill Adults Receiving Extracorporeal Membrane Oxygenation Support.

ASAIO J 2022 May 20. Epub 2022 May 20.

Neurocritical Care Division, Departments of Neurology, Neurosurgery, and Anesthesiology & Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Extracorporeal membrane oxygenation (ECMO) is an increasingly utilized intervention for cardiopulmonary failure. Analgosedation during ECMO support is essential to ensure adequate pain and agitation control and ventilator synchrony, optimize ECMO support, facilitate patient assessment, and minimize adverse events. Although the principles of analgosedation are likely similar for all critically ill patients, ECMO circuitry alters medication pharmacodynamics and pharmacokinetics. The lack of clinical guidelines for analgosedation during ECMO, especially at times of medication shortage, can affect patient management. Here, we review pharmacological considerations, protocols, and special considerations for analgosedation in critically ill adults receiving ECMO support.
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http://dx.doi.org/10.1097/MAT.0000000000001758DOI Listing
May 2022

Lactic acid bacteria isolated from Kazakh traditional fermented milk products affect the fermentation characteristics and sensory qualities of yogurt.

Food Sci Nutr 2022 May 19;10(5):1451-1460. Epub 2022 Apr 19.

China-Australia Joint Research Center for Dairy Future Technology Beijing Key Laboratory of Nutrition, Health & Food Safety Beijing Engineering Laboratory for Geriatric Nutrition Food Research COFCO Nutrition & Health Research Institute Beijing China.

Lactic acid bacteria (LAB) play a crucial role in the development of the taste, texture, and aroma of traditional fermented milk products. Five LABs from Kazakh traditionally prepared dairy products showed continuous subculture stability, as well as proper acidification and coagulation ability. They were identified as (1-5, 1-7), (1-19), and (1-12, 1-15). Their coagulation time and acidity values ranged from 5.97 to 12.78 h and 76.47 to 89.39°T. Yogurts prepared with were more condensed and textural integrity than those with and . Determination of the volatile compound profiles suggested a higher diversity of volatile compounds than the control. The sensory evaluation presented positive overall sensory quality scores for the yogurts prepared with 1-12 and 1-15. The results provide additional information regarding the contributions of native LABs to the unique flavor and sensory qualities of traditionally prepared milk products. They may help to select starters or adjunct starters for developing distinctive, traditional nomadic fermented milk to satisfy consumer demand and increase market acceptability.
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http://dx.doi.org/10.1002/fsn3.2755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094460PMC
May 2022

Effects of Protein Restriction and Succedent Realimentation on Jejunal Function and Bacterial Composition of Different Colonic Niches in Weaned Piglets.

Front Vet Sci 2022 3;9:877130. Epub 2022 May 3.

Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Recent studies have proved that protein succedent realimentation could rescue the loss of growth performance in weaning piglets caused by a prior protein restriction. However, how the protein restriction and succedent realimentation influence the jejunal function and bacterial composition of different colonic niches microbiota in weaning piglets needs a further investigation. After protein succedent realimentation, we found that the treatment group (TRE) piglets had a higher IGF-1 content and gene expression level in jejunal mucosa than the control group (CON) piglets. The gene expression level was up-regulated in the jejunal mucosa of TRE piglets during protein restriction and succedent realimentation, while the jejunal permeability of TRE piglets was only decreased after protein succedent realimentation. In addition, we found that protein restriction and succedent realimentation increased the gene expression of and the fecal apparent digestibility of crude protein in TRE piglets, but decreased the fecal nitrogen content. After 16S rRNA MiSeq sequencing of bacteria in different colonic niches (mucosa and digesta), TRE piglets had a higher relative abundance of beneficial bacteria and a lower relative abundance of potential pathogens than CON piglets in different colonic niches after protein restriction and succedent realimentation. Our data showed that protein restriction and succedent realimentation decreased the concentrations of branch chain fatty acids and ammonia-N in the colon of TRE piglets. In addition, protein succedent realimentation increased the concentration of total short chain fatty acids in the colon of TRE piglets. All these findings demonstrated that the strategy of protein restriction and succedent realimentation is an effective way to improve intestinal health of weaning piglets, and provided new insights into the nutrition management of piglets during the weaning period.
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http://dx.doi.org/10.3389/fvets.2022.877130DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111176PMC
May 2022

Design and Preparation of White High-Strength Concrete with Ground Limestone Powder by Means of Response Surface Methodology.

Materials (Basel) 2022 May 7;15(9). Epub 2022 May 7.

Institute of Building Materials, China Academy of Building Research, Beijing 100013, China.

This paper investigates the properties of white high-strength concrete (WHSC) prepared with ground limestone powder (GLP). Response surface methodology (RSM) was used to design the proportions of mixes and evaluate the influence of the water-binder ratio (w/b), slurry volume fraction (Vs), and the content of GLP in a binder (Cg) on the slump, whiteness and compressive strength of WHSC via Box-Behnken equations. Results indicate that quadratic polynomial regression equations can be used to predict the performance of WHSC as influenced by combined factors. Both slump and compressive strength of WHSC are found highly influenced by w/b while GLP significantly improves the whiteness of WHSC. An optimal mix proportion of WHSC is provided by the multi-objective optimization with high-accuracy predictions. This paper demonstrates the feasibility of preparing WHSC with GLP and presents the potential of using RSM in the mix proportioning of concrete.
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http://dx.doi.org/10.3390/ma15093359DOI Listing
May 2022

A Self-Driven Microfluidic Chip for Ricin and Abrin Detection.

Sensors (Basel) 2022 May 2;22(9). Epub 2022 May 2.

State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.

Ricin and abrin are phytotoxins that can be easily used as biowarfare and bioterrorism agents. Therefore, developing a rapid detection method for both toxins is of great significance in the field of biosecurity. In this study, a novel nanoforest silicon microstructure was prepared by the micro-electro-mechanical systems (MEMS) technique; particularly, a novel microfluidic sensor chip with a capillary self-driven function and large surface area was designed. Through binding with the double antibodies sandwich immunoassay, the proposed sensor chip is confirmed to be a candidate for sensing the aforementioned toxins. Compared with conventional immunochromatographic test strips, the proposed sensor demonstrates significantly enhanced sensitivity (≤10 pg/mL for both toxins) and high specificity against the interference derived from juice or milk, while maintaining good linearity in the range of 10-6250 pg/mL. Owing to the silicon nanoforest microstructure and improved homogeneity of the color signal, short detection time (within 15 min) is evidenced for the sensor chip, which would be helpful for the rapid tracking of ricin and abrin for the field of biosecurity.
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http://dx.doi.org/10.3390/s22093461DOI Listing
May 2022

[Mechanism of microRNA-100-5p on mammalian target of rapamycin in temporomandibular arthritis].

Shanghai Kou Qiang Yi Xue 2022 Feb;31(1):12-16

Department of Stomatology, Ji'an Central People's Hospital of Jiangxi Province. Ji'an 343000, China. E-mail:

Purpose: To investigate the mechanism of microRNA-100-5p (miR-100-5p) on mammalian target (mTOR) of rapamycin in temporomandibular arthritis.

Methods: Sixty SD rats were randomly divided into group A, group B, group C, group D, and group E, with 12 rats in each group. Rat models of temporomandibular arthritis were prepared by injecting sodium iodoacetate solution into the bilateral spaces of temporomandibular joint. After establishment, group C was injected pcDNA3.1-miR-100-5p recombinant plasmid, group D was injected mTOR inhibitor rapamycin, group E was injected with pcDNA3.1-miR-100-5p recombinant plasmid and rapamycin, and group A was injected same amount of normal saline in the same way. Various indexes were observed in each group, including morphological changes of temporomandibular joint tissues, matrix metalloproteinase-3 (MMP-3), MMP-1, MMP-13, interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), miR-100-5p, mTOR expression. The data were processed using SPSS 22.0 software package.

Results: In group B, the structure of temporomandibular joint was fuzzy, with synovial hyperplasia, vascular dilatation, clustered cells and a large amount of inflammatory infiltration. Histopathological changes of temporomandibular joint in each interventional group were improved to different degrees compared with group B, among which group E showed the most obvious improvement. The levels of MMP-3, MMP-1, MMP-13, IL-6, IL-1β and TNF-α in group B were significantly higher than those in group A(P<0.05). The levels of MMP-3, MMP-1, MMP-13, IL-6, IL-1β and TNF-α in group C, group D and group E were significantly lower than those in group B(P<0.05). The levels of MMP-3, MMP-1, MMP-13, IL-6, IL-1β and TNF-α in group D were not significantly different from those in group C (P<0.05). The levels of MMP-3, MMP-1, MMP-13, IL-6, IL-1β and TNF-α in group E were significantly lower than those in group D (P<0.05). The expression level of miR-100-5p in group E was significantly higher than that in group B (P<0.05). The expression level of mTOR protein in group E was significantly lower than that in group B (P<0.05).

Conclusions: MicroRNA-100-5p may alleviate temporomandibular arthritis by down-regulating the expression of mTOR.
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February 2022

Digestion characteristics of quinoa, barley and mungbean proteins and the effects of their simulated gastrointestinal digests on CCK secretion in enteroendocrine STC-1 cells.

Food Funct 2022 May 19. Epub 2022 May 19.

School of Health Science and Engineering, National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, University of Shanghai for Science and Technology, Shanghai 200093, China.

The demand for plant-based proteins has been rapidly increasing due to sustainability, ethical and health reasons. The present study aimed to investigate the digestion characteristics of three plant proteins (quinoa, barley and mungbean) based on an digestion model and the effect of their simulated gastrointestinal digests on satiety hormone cholecystokinin (CCK) secretion in enteroendocrine STC-1 cells. The nitrogen distribution in the digestion process, the relative molecular weight (MW) of peptides and the amino acid composition in simulated gastrointestinal digests were characterized. Quinoa protein had the highest proportion of soluble nitrogen after gastrointestinal digestion (85.79%), followed by barley protein (74.98%) and mungbean protein (64.14%), suggesting that quinoa protein was more easily digested than barley and mungbean proteins. The peptides but not free amino acids were the main components in the gastrointestinal digests of quinoa, barley, and mungbean proteins. The gastrointestinal digest of quinoa protein had a well balanced amino acid pattern, whereas that of barley protein was lacking Lys, and that of the mungbean protein was short of sulfur amino acids (Phe + Tyr) but rich in Lys. In terms of the ability to stimulate CCK secretion, the gastrointestinal digest of barley protein had a strong stimulatory effect on CCK secretion, while that of quinoa and mungbean proteins had only a weak stimulatory effect. After pretreatment with a specific calcium-sensing receptor (CaSR) antagonist NPS 2143, CCK secretion induced by the barley protein digest was greatly suppressed, indicating that CaSR was involved in barley protein digest-induced CCK secretion. These results show that quinoa protein has good nutritional quality, while barley protein is an excellent plant protein source to stimulate CCK secretion and has a potential application as a dietary supplement for obesity management.
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http://dx.doi.org/10.1039/d2fo00243dDOI Listing
May 2022

Horizontal ridge augmentation in the anterior maxilla with in situ onlay bone grafting: a retrospective cohort study.

Clin Oral Investig 2022 May 18. Epub 2022 May 18.

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section, Renmin Nan Road, Chengdu, 610041, Sichuan, China.

Objectives: This study aimed to introduce a digitally guided in situ autogenous onlay grafting technique and compare its effectiveness with the conventional (ex situ) onlay technique in augmenting horizontal bone defects of the anterior maxilla.

Materials And Methods: This retrospective cohort study included 24 patients who had received autogenous onlay bone grafts combined with guided bone regeneration (GBR) in the anterior maxilla. Fourteen patients were recruited into the in situ onlay grafting group (EG), and 10 were recruited into the ex situ onlay group (CG), defined by the donor sites. The clinical parameters, radiographic changes, micro-CT, and histological processes were evaluated after a mean follow-up period of 1.7 years.

Results: The horizontal bone width reflected significant bone modeling over time (p < 0.001) in the first 6 months. Multivariable analysis showed that the treatment modality (grouping) was a critical factor positively associated with vertical bone height alteration. However, neither the alteration rate of horizontal bone width nor the bone volume was associated with the treatment modality. The number of periosteal screws per graft positively affected horizontal contour maintenance (p < 0.05). No significant differences were observed between the groups in the clinical parameters (complications, success rate, and peri-implant parameters). The micro-CT and histological outcomes were similar between the groups.

Conclusion: Despite the limitations of this study, in situ onlay grafting combined with GBR was an effective and reliable approach for horizontal bone augmentation in the anterior maxilla and appeared to demonstrate better stability in vertical bone remodeling.

Clinical Relevance: This study introduces a modified and minimally invasive technique of onlay grafting for horizontal bone augmentation. This in situ onlay grafting demonstrates superior stability in vertical bone remodeling. The trial registration number is ChiCTR2100054683.
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http://dx.doi.org/10.1007/s00784-022-04547-1DOI Listing
May 2022

Salidroside alleviates cadmium-induced toxicity in mice by restoring the notch/HES-1 and RIP1-driven inflammatory signaling axis.

Inflamm Res 2022 May 18. Epub 2022 May 18.

Department of Cell Biology and Medical Genetics, School of Basic Medical Sciences, Shenzhen University Health Science Center, Shenzhen, 518060, China.

Objective: Salidroside (SAL) is a marker glycoside of Rhodiola rosea with significant antioxidant, anti-inflammatory, and other health benefits. In this study, we determined its neuroprotective effects against Cd-induced toxicity in cultured cells and mice.

Materials And Methods: GL261 cell and Cd-intoxicated mouse model were used. ICP-MS and MWM were performed to measure Cd content and Cd-induced cognitive impairment in mice, respectively.

Results: SAL attenuated Cd toxicity in GL261 cells as well as protected mice from substantial organic damage and cognitive deficits. SAL treatment alleviated Cd-induced oxidative stress, glial cell activation, and elevation of pro-inflammatory factors including TNF-α, IL-1β, and IL-6. Cd-induced cognitive deficits observed in the Morris water maze in mice were rescued by SAL. At the mechanistic level, SAL maintained the activity of antioxidant enzymes such as SOD and GSH-Px in the serum and brain, and scavenged the peroxidation product MDA, thereby restoring redox homeostasis in vivo, attenuating neuronal damage, and ultimately antagonized Cd-induced toxicity. Furthermore, Cd activated the RIP1-driven inflammatory signaling pathway and Notch/HES-1 signaling axis in the brain, leading to inflammation and neuronal loss, which could be attenuated by SAL.

Conclusion: SAL is a natural product with good anti-Cd effects, indicating that Rhodiola rosea is promising plant that is worthy of cultivation for health and economic benefits.
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http://dx.doi.org/10.1007/s00011-022-01580-4DOI Listing
May 2022

sp. nov., a methylotrophic methanogen isolated from sediment of the East China Sea.

Int J Syst Evol Microbiol 2022 May;72(5)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China.

A novel methylotrophic methanogen sp. nov. was isolated from East China Sea sediment. Type strain LMO-1 of sp. nov. was irregular 1-2 µm cocci without flagella. Strain LMO-1 could utilize a variety of methylated compounds including methanol, methylamine, dimethylamine and trimethylamine for growth and methanogenesis, while H/CO or acetate could not be used for growth or methanogenesis. Optimum growth temperature was 30-35 °C, optimum pH range for growth was 7.0-7.5, while the optimum salinity spectrum for growth was 1.0%-5.0% NaCl. Based on 16S rRNA gene similarity, strain LMO-1 belongs to , with the highest sequence similarity to DSM 2657 (99.8 %), SLH33(99.4 %), followed by AK-5(98.1 %), DSM 6242 (98.0 %). Digital DNA-DNA hybridization also showed highest similarity with DSM 2657, with the value of 58.4 %. The average nucleotide identity between strain LMO-1 and DSM 2657 was 94.06 %. In summary, LMO-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LMO-1 (=MCCC 4K00106=JCM 39195).
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http://dx.doi.org/10.1099/ijsem.0.005384DOI Listing
May 2022

[IL-6 promotes the activation of rat astrocytes and down-regulation of the expression of Kir4.1 channel].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2022 Apr;38(4):316-320

Department of Histology and Embryology, Bengbu Medical College, Bengbu 233030, China. *Corresponding author, E-mail:

Objective To identify the effects of interleukin-6 (IL-6) on astrocytes activation, and the regulation of the expression of inwardly rectifying potassium 4.1 (Kir4.1) channels in astrocytes. Methods Astrocytes were separated from the cerebral cortex of newborn SD rats, and cultured in the presence of IL-6 or combined with interleukin-6 receptor antagonist (IL-6Ra). CCK-8 assay was performed to measure cell viability. The expression level of Kir4.1 channels in astrocytes was measured using quantitative real-time PCR and Western blot analysis. Results IL-6 promoted the proliferation of astrocytes in a dose- (0-30 ng/mL) and time- (0-24 hours) dependent manner. After astrocytes were treated with IL-6 (30 ng/mL) for 24 hours, the levels of Kir4.1 mRNA and protein decreased significantly, and this down-regulation could be attenuated by IL-6Ra. Conclusion IL-6 promotes the activation of astrocyte and down-regulation of the expression of Kir4.1 channel.
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April 2022

Cardiac disorder-related adverse events for aryl hydrocarbon receptor agonists: a safety review.

Expert Opin Drug Saf 2022 May 18. Epub 2022 May 18.

Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: Although cardiac disorder-related adverse events (AEs) have been reported in patients treated with aryl hydrocarbon receptor (AHR) agonists, their safety profiles remain unknown. Here, we identified significant cardiac disorders associated with AHR agonists and further evaluated their relevance.

Research Design And Methods: Database queries were performed using OpenVigil 2.1 and AEs voluntarily submitted to Food and Drug Administration Adverse Event Reporting System (FAERS) between 2004 and 2020 were included. This study based on the Medical Dictionary for Regulatory Activities and the standardized MedDRA Queries to define the preferred terms, and we used reporting odd ratio to detect signals.

Results: In the FAERS database, 14,078 cardiac disorder-related AEs were identified in patients receiving AHR agonists. Among all AHR agonists, the number of cardiac disorder-related PTs with positive signals for AHR agonists was 93. Peripheral swelling (n=1572) and atrial fibrillation (n=1277) were the most reported cardiac disorder-related AEs among AHR agonists in disproportionately reported PTs. Moreover, several AHR agonists were highly associated with tachyarrhythmia.

Conclusions: By mining the FAERS database, we provided more information on the association between AHR agonist use and cardiac disorder-related AEs.
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http://dx.doi.org/10.1080/14740338.2022.2078301DOI Listing
May 2022

Small nucleolar RNA host gene 3 functions as a novel biomarker in liver cancer and other tumour progression.

World J Gastroenterol 2022 Apr;28(16):1641-1655

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, Zhejiang Province, China.

Cancer has become the most life-threatening disease in the world. Mutations in and aberrant expression of genes encoding proteins and mutations in noncoding RNAs, especially long noncoding RNAs (lncRNAs), have significant effects in human cancers. LncRNAs have no protein-coding ability but function extensively in numerous physiological and pathological processes. Small nucleolar RNA host gene 3 (SNHG3) is a novel lncRNA and has been reported to be differentially expressed in various tumors, such as liver cancer, gastric cancer, and glioma. However, the interaction mechanisms for the regulation between SNHG3 and tumor progression are poorly understood. In this review, we summarize the results of SNHG3 studies in humans, animal models, and cells to underline the expression and role of SNHG3 in cancer. SNHG3 expression is upregulated in most tumors and is detrimental to patient prognosis. SNHG3 expression in lung adenocarcinoma remains controversial. Concurrently, SNHG3 affects oncogenes and tumor suppressor genes through various mechanisms, including competing endogenous RNA effects. A deeper understanding of the contribution of SNHG3 in clinical applications and tumor development may provide a new target for cancer diagnosis and treatment.
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http://dx.doi.org/10.3748/wjg.v28.i16.1641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048787PMC
April 2022

Selenium Alleviates Ammonia-Induced Splenic Cell Apoptosis and Inflammation by Regulating the Interleukin Family/Death Receptor Axis and Nrf2 Signaling Pathway.

Biol Trace Elem Res 2022 May 18. Epub 2022 May 18.

College of Life Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China.

Ammonia (NH) is a harmful gas in livestock houses. So far, many researchers have demonstrated that NH is detrimental to animal and human organs. Selenium (Se) is one of the essential trace elements in the body and has a good antioxidant effect. However, there was little conclusive evidence that Se alleviated NH poisoning. To investigate the toxic mechanism of NH on pig spleen and the antagonistic effect of L-selenomethionine, a porcine NH-poisoning model and an L-selenomethionine intervention model were established in this study. Our results showed that NH exposure increased the apoptosis rate, while L-selenomethionine supplementation alleviated the process of excessive apoptosis. Immunofluorescence staining, real-time quantitative polymerase chain reaction (qRT-PCR), and western blot results confirmed that exposure to NH changed the expression levels of interleukin family factors, apoptosis, death receptor, and oxidative stress factors. Our study further confirmed that excessive NH induced inflammatory response and mediated necroptosis leading to cell apoptosis by activating the Nrf2 signaling pathway. Excessive NH could mediate spleen injury through oxidative stress-induced mitochondrial dynamics disorder. L-Selenomethionine could alleviate inflammation and abnormal apoptosis by inhibiting the IL-17/TNF-α/FADD axis. Our study would pave the way for comparative medicine and environmental toxicology.
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http://dx.doi.org/10.1007/s12011-022-03279-3DOI Listing
May 2022

Meaning in Life and Self-Control Mediate the Potential Contribution of Harsh Parenting to Adolescents' Problematic Smartphone Use: Longitudinal Multi-Group Analyses.

J Interpers Violence 2022 May 17:8862605221099495. Epub 2022 May 17.

School of Education, 12471Renmin University of China, Beijing, China.

Problematic smartphone use (PSU) has become an increasingly serious social issue that gradually impairs adolescents' daily social functioning. This study aimed to examine the potential contribution of harsh parenting (HP) to PSU by testing a two-mediator model in which meaning in life (MIL) and self-control (SC) were hypothesized to be two mediators. The moderating role of gender was also examined. Eight hundred and twenty-eight middle school students from rural areas in China (mean age = 13.04) reported on harsh parenting, MIL, self-control, and PSU. Both parents also reported on each other's harsh parenting and their child's self-control. Multi-group structural equation modeling analyses revealed gender differences in the association patterns among the model variables. Harsh parenting was only indirectly associated with PSU for both boys and girls. MIL and self-control completely mediated the relation between harsh parenting and PSU in sequential manners for boys and girls. And for boys, beyond the common path from harsh parenting to PSU, another indirect path existed from harsh parenting to self-control to PSU. But jointly, MIL and self-control precipitate more indirect effects for girls than for boys in the association between harsh parenting and PSU. Findings suggested that harsh parenting was detrimental to adolescents' MIL and self-control, which enhances their risk for PSU, especially for girls. These findings provide more insights for efforts to prevent adolescents from PSU.
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http://dx.doi.org/10.1177/08862605221099495DOI Listing
May 2022

Prediction Model of Photodegradation for PBAT/PLA Mulch Films: Strategy to Fast Evaluate Service Life.

Environ Sci Technol 2022 May 17. Epub 2022 May 17.

College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China.

Eco-friendly biodegradable PBAT/PLA mulch films are attracting increasing interest in sustainable agricultural production. However, currently, little is known about the service life for the PBAT/PLA mulch films. Herein, PBAT/PLA mulch films are subjected to indoor UV-accelerated degradation (UAD) experiments and field cultivation environment degradation (CED) experiments to systematically investigate the relationship between UAD and CED processes. Results demonstrate that 10 days of indoor UAD treatment corresponds to around 120 days aging under outdoor CED conditions. Using eight PBAT/PLA evaluation indicators (haze, elongation at break, tensile strength, gel content, light transmittance, polydispersity index, , ), we established a service life prediction model for PBAT/PLA mulch films based on short-term indoor UAD experiments, which could accurately estimate the long-term service life of the mulch films in the field. In particular, using the haze value, near-perfect correlation ( = 0.995 for eq. 1 and = 0.993 for eq. 2) was found between CED days and UAD days. The establishment of these reliable predictive models for the service lifetime of PBAT/PLA mulch films will avoid the undesirable premature breakdown during crop growth, thus fostering end-user confidence in eco-friendly biodegradable mulch films.
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http://dx.doi.org/10.1021/acs.est.2c01687DOI Listing
May 2022

Immune Subtype Profiling and Establishment of Prognostic Immune-Related lncRNA Pairs in Human Ovarian Cancer.

Comput Math Methods Med 2022 5;2022:8338137. Epub 2022 May 5.

Department of Gynecology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, No. 44 Xiaoheyan Road, Dadong District, Shenyang, 110042 Liaoning Province, China.

This study collected immune-related genes (IRGs) and used gene expression data from TCGA database to construct a molecular subtype of ovarian cancer (OV) based on immune-related lncRNA gene pairs (IRLnc_GPs). The relationships between molecular subtypes and prognosis and clinical characteristics were further explored. IRGs were acquired from the ImmPort database, and round-robin pairing of immune-related lncRNAs was performed. The NMF algorithm was used to identify molecular subtypes, and the immune score of a single sample was calculated through ESTIMATE, TIMER, ssGSEA, MCPcounter, and CIBERSORT. The relationship between molecular subtypes and immune microenvironments was identified. A hypergeometric test was used to test the lncRNA pairs among the OV molecular subtypes (C1 and C2 subtypes). The BH method was used to screen the different lncRNA pairs, and a predictive risk model was constructed and verified. Finally, correlation analysis between the risk model, immune checkpoint genes, and chemotherapy drugs was carried out. Based on IRLnc_GP to classify 373 OV samples of TCGA, the samples were divided into two subtypes, and the prognosis between the subtypes showed significant differences. The C1 subtype with a poor prognosis was more related to the pathways of tumor occurrence and development. We identified 180 differential lncRNA pairs between subtypes and constructed a prognostic risk model based on 8 IRLnc_GPs. In the independent dataset, the distribution of subtypes in functional modules was different and highly repeatable. There were significant differences in the molecular and clinical characteristics of the subtypes and the drug sensitivity of immunotherapy/chemotherapy. In conclusion, the risk model established based on IRLnc_GP can better evaluate the prognosis of OV samples and can also assess the effects of different drug treatments in the high- and low-risk groups, providing new insights and ideas for the treatment of OV.
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http://dx.doi.org/10.1155/2022/8338137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107039PMC
May 2022

The biallelic novel pathogenic variants in AGL gene in a chinese patient with glycogen storage disease type III.

BMC Pediatr 2022 May 16;22(1):284. Epub 2022 May 16.

Tianjin Children's Hospital (Children's Hospital of Tianjin University), 300134, Tianjin, China.

Background: Glycogen storage disease type III (GSD III) is a rare autosomal recessive glycogenolysis disorder due to AGL gene variants, characterized by hepatomegaly, fasting hypoglycemia, hyperlipidemia, elevated hepatic transaminases, growth retardation, progressive myopathy, and cardiomyopathy. However, it is not easy to make a definite diagnosis in early stage of disease only based on the clinical phenotype and imageology due to its clinical heterogeneity.

Case Presentation: We report a two-year-old girl with GSD III from a nonconsanguineous Chinese family, who presented with hepatomegaly, fasting hypoglycemia, hyperlipidemia, elevated levels of transaminases. Accordingly, Sanger sequencing, whole‑exome sequencing of family trios, and qRT-PCR was performed, which revealed that the patient carried the compound heterogeneous variants, a novel frameshift mutation c.597delG (p. Q199Hfs*2) and a novel large gene fragment deletion of the entire exon 13 in AGL gene. The deletion of AGL was inherited from the proband's father and the c.597delG variant was from the mother.

Conclusions: In this study, we identified two novel variants c.597delG (p. Q199Hfs*2) and deletion of the entire exon 13 in AGL in a Chinese GSD III patient. We extend the mutation spectrum of AGL. We suggest that high-throughput sequencing technology can detect and screen pathogenic variant, which is a scientific basis about genetic counseling and clinical diagnosis.
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http://dx.doi.org/10.1186/s12887-022-03252-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109368PMC
May 2022

Corrigendum to "RNA sequencing reveals target genes of temporomandibular joint osteoarthritis in rats after the treatment of low-intensity pulsed ultrasound" [Gene 672 (2018) 126-136].

Gene 2022 May 13;830:146532. Epub 2022 May 13.

Department of Stomatology, Jinan Military General Hospital, Jinan, Shandong Province, People's Republic of China.

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http://dx.doi.org/10.1016/j.gene.2022.146532DOI Listing
May 2022

Bilateral Cerebellar Intermittent Theta Burst Stimulation Combined With Swallowing Speech Therapy for Dysphagia After Stroke: A Randomized, Double-Blind, Sham-Controlled, Clinical Trial.

Neurorehabil Neural Repair 2022 May 16:15459683221092995. Epub 2022 May 16.

Department of Rehabilitation Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.

Background: Previous studies have found that high-frequency repetitive transcranial magnetic stimulation (rTMS) of the cerebellar hemisphere could improve swallowing function, but whether intermittent theta burst stimulation (iTBS), which has similar excitatory effect and higher efficiency, can also improve swallowing function for dysphagia after stroke remains unclear.

Objective: This trial aimed to explore the efficacy and safety of bilateral cerebellar transcranial magnetic stimulation with iTBS for dysphagia after stroke.

Methods: Seventy patients with dysphagia after stroke were divided into 2 treatment groups: true bilateral cerebellar iTBS and sham bilateral cerebellar iTBS. The true iTBS group underwent ten 100% resting motor threshold (RMT) iTBS sessions for 2 weeks. In the sham iTBS group, the parameters were the same except that the figure-eight coil was perpendicular to the skull. Both groups received traditional swallowing rehabilitation treatment 5 times a week for 2 weeks. Swallowing function was assessed with the Fiberoptic Endoscopic Dysphagia Severity Scale (FEDSS), Penetration/Aspiration Scale (PAS), Standardized Swallowing Assessment (SSA), and Functional Oral Intake Scale (FOIS) at baseline, 2 weeks after the intervention, and at 4 weeks of follow-up.

Results: There were significant time and group interaction effects in both multi-factorial adjusted and unadjusted FEDSS, PAS, SSA, and FOIS score ( < .001). In the pairwise comparison of the swallowing parameters among the 2 groups, the FEDSS, PAS, SSA, and FOIS scores at 2 weeks and 4 weeks showed a significantly higher improvement in the iTBS simulation group than sham group ( < .05). In both the true iTBS and sham iTBS stimulation groups, all FEDSS, PAS, SSA, and FOIS scores were significantly improved over time ( < .001).

Conclusions: The present study suggested that as a more efficient TMS stimulation mode, iTBS could efficiently improve swallowing function by stimulating the bilateral cerebellar hemisphere. In addition, 100% resting motor threshold bilateral cerebellar iTBS is a relatively safe treatment.

Clinical Trial Registration: Effect analysis of repeated transcranial magnetic stimulation of cerebellar on dysphagia after stroke. www.chictr.org.cn. Identifier: ChiCTR2100042092.
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http://dx.doi.org/10.1177/15459683221092995DOI Listing
May 2022

Animal Models of Temporomandibular Joint Osteoarthritis: Classification and Selection.

Front Physiol 2022 28;13:859517. Epub 2022 Apr 28.

Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.

Temporomandibular joint osteoarthritis (TMJOA) is a common degenerative joint disease that can cause severe pain and dysfunction. It has a serious impact on the quality of lives of patients. Since mechanism underlying the pathogenesis of TMJOA is not fully understood, the development of effective tools for early diagnosis and disease-modifying therapies has been hindered. Animal models play a key role in understanding the pathological process of diseases and evaluating new therapeutic interventions. Although some similarities in disease processes between animals and humans are known, no one animal model is sufficient for studying all characteristics of TMJOA, as each model has different translatability to human clinical conditions. For the past 4 decades, TMJOA animal models have been studied by numerous researchers and can be broadly divided into induced, naturally occurring, and genetically modified models. The induced models can be divided into invasive models (intra-articular injection and surgical induction) or non-invasive models (mechanical loading, high-fat diet, and sleep deprivation). Different types of animal models simulate different pathological expressions of TMJOA and have their unique characteristics. Currently, mice, rats, and rabbits are commonly used in the study of TMJOA. This review sought to provide a general description of current experimental models of TMJOA and assist researchers in selecting the most appropriate models for different kinds of research.
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http://dx.doi.org/10.3389/fphys.2022.859517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095932PMC
April 2022

Research Progress and Trends in Metabolomics of Fruit Trees.

Front Plant Sci 2022 29;13:881856. Epub 2022 Apr 29.

Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Metabolomics is an indispensable part of modern systems biotechnology, applied in the diseases' diagnosis, pharmacological mechanism, and quality monitoring of crops, vegetables, fruits, etc. Metabolomics of fruit trees has developed rapidly in recent years, and many important research results have been achieved in combination with transcriptomics, genomics, proteomics, quantitative trait locus (QTL), and genome-wide association study (GWAS). These research results mainly focus on the mechanism of fruit quality formation, metabolite markers of special quality or physiological period, the mechanism of fruit tree's response to biotic/abiotic stress and environment, and the genetics mechanism of fruit trait. According to different experimental purposes, different metabolomic strategies could be selected, such as targeted metabolomics, non-targeted metabolomics, pseudo-targeted metabolomics, and widely targeted metabolomics. This article presents metabolomics strategies, key techniques in metabolomics, main applications in fruit trees, and prospects for the future. With the improvement of instruments, analysis platforms, and metabolite databases and decrease in the cost of the experiment, metabolomics will prompt the fruit tree research to achieve more breakthrough results.
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http://dx.doi.org/10.3389/fpls.2022.881856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106391PMC
April 2022

Clinical Profile and Risk Factors for Cardiac Death in Pediatric Patients With Primary Dilated Cardiomyopathy at a Tertiary Medical Center in China.

Front Pediatr 2022 28;10:833434. Epub 2022 Apr 28.

Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Aim: We sought to identify the clinical characteristics and risk factors for cardiac mortality in pediatric patients with primary dilated cardiomyopathy (DCM) in China.

Methods: A total of 138 pediatric patients who were consecutively diagnosed with primary DCM from January 2011 to December 2020 were included. We assessed patients' clinical symptoms and performed laboratory examinations, electrocardiography, and echocardiography.

Results: Of these patients, 79 (57%) had severe systolic dysfunction (left ventricular ejection fraction of < 30%), 79 (57.2%) developed DCM before 12 months of age, 62 (45%) were male, 121 (87.7%) presented with advanced heart failure (cardiac functional class III/IV), and 54 (39.1%) presented with arrhythmia. At a median follow-up of 12 months, the overall cardiac mortality rate was 33%, and 40 of 46 deaths occurred within 6 months following DCM diagnosis. A multivariate Cox regression analysis identified several independent cardiac death predictors, including an age of 12 months to 5 years [hazard ratio (HR) 2.799; 95% confidence interval (CI) 1.160-6.758; = 0.022] or 10-15 years (HR 3.617; 95% CI 1.336-9.788; = 0.011) at diagnosis, an elevated serum alanine aminotransferase (ALT) concentration (≥ 51.5 U/L) (HR 2.219; 95% CI 1.06-4.574; = 0.031), and use of mechanical ventilation (HR 4.223; 95% CI 1.763-10.114; = 0.001).

Conclusion: The mortality rate of primary DCM without transplantation is high. Age, an elevated serum ALT concentration, and the need for mechanical ventilation predict mortality in patients with primary DCM, providing new insights into DCM risk stratification.
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http://dx.doi.org/10.3389/fped.2022.833434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096786PMC
April 2022

Barriers and Facilitators for Smoking Cessation in Chinese Smokers with Chronic Obstructive Pulmonary Disease: A Qualitative Study.

Int J Chron Obstruct Pulmon Dis 2022 6;17:1107-1120. Epub 2022 May 6.

Centre for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing, 100029, People's Republic of China.

Background: Smoking cessation is recommended as a key intervention for chronic obstructive pulmonary disease (COPD) smokers. However, in China, few COPD smokers quit successfully. The aim of this study was to explore in depth the barriers and facilitators for smoking cessation among smokers with COPD in China.

Methods: A purposive sample of 32 hospitalized smokers with COPD were included, 17 ex-smokers and 15 current smokers, participated in the semi-structured interviews. Interviews were analyzed thematically and using a deductive approach guided by Capability, Opportunity, Motivation-Behavior (COM-B) framework.

Results: Three inter-related themes were generated: smokers' motivation was a prerequisite for quitting, maintaining capability to quit smoking, and opportunities that facilitated smokers to quit. Motivation to quit for most participants was activated by COPD-related symptoms, although they had a limited knowledge of COPD. Physical benefits from quitting and strong willpower were facilitators for maintaining quitting, while exposure to smoking environment and strong addiction to nicotine were frequent reasons for relapse. Most ex-smokers quit smoking by their own willpower rather than professionally delivered smoking cessation interventions. Smokers' attitudes toward these interventions depended on their effectiveness and convenience. Very few participants had experienced pharmacotherapy or behavioral support from physicians. However, interviewees preferred auricular acupressure to pharmacotherapy.

Conclusion: Motivation to quit among smokers with COPD was usually initiated by COPD-related symptoms. Physical benefits observed by quitting and strong willpower facilitated smoking cessation, while exposure to smoking environment and strong addiction to nicotine led to relapse. COPD smokers in China preferred auricular acupressure to pharmacotherapy.
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http://dx.doi.org/10.2147/COPD.S356935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091319PMC
May 2022

COVID-19 and Sudden Sensorineural Hearing Loss: A Systematic Review.

Front Neurol 2022 28;13:883749. Epub 2022 Apr 28.

Department of Otolaryngology, Wuxi Huishan District People's Hospital, Wuxi, China.

A growing body of evidence suggests that patients with the 2019 Coronavirus disease (COVID-19) have a risk of developing sudden sensorineural hearing loss (SSNHL). The pathogenesis of COVID-19-related SSNHL remains unclear. This systematic review examined whether COVID-19 causes an increased incidence of SSNHL and the clinical characteristics of patients with COVID-19-related SSNHL according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. SSNHL usually developed between a few days and 2 months after the diagnosis of COVID-19, and a proportion of patients developed it before the diagnosis of COVID-19. The literature is inconsistent regarding whether COVID-19 causes an increased incidence of SSNHL, and this matter remains unclear. This review included 23 patients with COVID-19-related SSNHL, all adult patients with an average age of 43.1 years. Of these patients, 60.9% had accompanying tinnitus symptoms. Glucocorticoids are the preferred medication to treat COVID-19-related SSNHL. Intratympanic administration may be considered to reduce the side effects of the drug. Hearing tests are suggested when hearing loss is suspected in COVID-19 individuals, and if SSNHL is detected, prompt and aggressive treatment is vital. Large-scale, multicenter research on the pathophysiology, treatment, and prognosis of COVID-19- related SSNHL should be conducted in the future.
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http://dx.doi.org/10.3389/fneur.2022.883749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096262PMC
April 2022

Effects of Site-Directed Mutagenesis of Cysteine on the Structure of Sip Proteins.

Front Microbiol 2022 29;13:805325. Epub 2022 Apr 29.

College of Life Science, Northeast Agricultural University, Harbin, China.

, a gram-positive bacteria, has three insecticidal proteins: Vip (vegetative insecticidal protein), Cry (crystal), and Sip (secreted insecticidal protein). Of the three, Sip proteins have insecticidal activity against larvae of Coleoptera. However, the Sip1Aa protein has little solubility in the supernatant because of inclusion bodies. This makes it more difficult to study, and thus research on Sip proteins is limited, which hinders the study of their mechanistic functions and insecticidal mechanisms. This highlights the importance of further investigation of the Sip1Aa protein. Disulfide bonds play an important role in the stability and function of proteins. Here, we successfully constructed mutant proteins with high insecticidal activity. The tertiary structure of the Sip1Aa protein was analyzed with homologous modeling and bioinformatics to predict the conserved domain of the protein. Cysteine was used to replace amino acids via site-directed mutagenesis. We successfully constructed Sip149-251, Sip153-248, Sip158-243, and Sip178-314 mutant proteins with higher solubility than Sip1Aa. Sip153-248 and Sip158-243 were the most stable compared to Sip1Aa, followed by Sip149-251 and Sip178-314. The insecticidal activity of Sip153-248 (Sip158-243) was 2.76 (2.26) times higher than that of Sip1Aa. The insecticidal activity of Sip149-251 and Sip178-314 did not differ significantly from that of Sip1Aa. Basic structural properties, physicochemical properties, and the spatial structure of the mutation site of Sip1Aa and the mutant proteins were analyzed. These results provide a molecular basis for using Sip1Aa to control Coleopteran insects and contribute to the study of the Sip1Aa insecticidal mechanism.
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http://dx.doi.org/10.3389/fmicb.2022.805325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100928PMC
April 2022
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