Publications by authors named "Jing Tang"

802 Publications

Conjugated Cationic Pp- Formed on g-CN for Photocatalyzed Water Splitting.

Langmuir 2021 Jun 14. Epub 2021 Jun 14.

State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Southwest Petroleum University, Chengdu, Sichuan 610500, P. R. China.

Polycationic [email protected] composite was synthesized through an in situ polymerization process of -alkylpyridinium acetylenic alcohol bromide (p-) above the surface of g-CN. The structure of p-0 and the [email protected] properties were checked by modern technologies. Photocatalytic tests of [email protected] in water splitting unveiled much better [email protected] hydrogen evolution activities by comparison with both g-CN and Pp-0. The hydrogen production by [email protected] was 1654.5 μmol h g, which is ∼26- and 22-fold greater in relation to what g-CN and Pp-0 produced (62.7 and 75.0 μmol h g, respectively), suggesting strong bilateral and synergistic interactions of g-CN with Pp-0. Although the lengthening methylene chain in the polymers weakened the hydrogen generation ability of [email protected], the conjugated double bonds, solubilization, and dispersion of Pp- polycationic surfactants made [email protected] superior to g-CN in water splitting. Due to the readily available raw materials, a simple way of preparation (starting chemicals to p-0 to [email protected]), high photocatalysis efficiency, light irritation stability, recyclable ability, and low toxicity, [email protected] is a good candidate for water splitting.
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http://dx.doi.org/10.1021/acs.langmuir.1c00594DOI Listing
June 2021

Dictionary learning constrained direct parametric estimation in dynamic myocardial perfusion PET.

IEEE Trans Med Imaging 2021 Jun 14;PP. Epub 2021 Jun 14.

In myocardial perfusion imaging with dynamic positron emission tomography (PET), direct parametric reconstruction from the projection data allows accurate modeling of the Poisson noise in the projection domain to provide more reliable estimate of the parametric images. In this study, we propose to incorporate a superior denoiser to efficiently suppress the unfavorable noise propagation during the direct reconstruction. The dictionary learning (DL) based sparse representation serves as a regularization term to constrain the intermediate K1 estimation. We rewrite the DL regularizer into a voxel-separable form to facilitate the decoupling of a DL penalized curve fitting from the reconstruction of dynamic frames. The nonlinear fitting is then solved by a damped Newton method with uniform initialization. Using simulated and patient 82Rb dynamic PET data, we study the performance of the proposed DL direct algorithm and quantitatively compare it with the indirect method with or without post-filtering, the direct reconstruction without regularization, and the quadratic penalty regularized direct algorithm. The DL regularized direct reconstruction achieves improved noise versus bias performance in the reconstructed K1 images as well as superior recovery of reduced myocardial blood flow defect. The dictionary learned from a 3D self-created hollow sphere image yields comparable results to those using the dictionary learned from the corresponding MR image. The uniform initialization has been shown to converge to similar K1 estimation to the result from initializing with the indirect reconstruction. To summarize, we demonstrate the potential of the proposed DL constrained direct parametric reconstruction in improving quantitative dynamic PET imaging.
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http://dx.doi.org/10.1109/TMI.2021.3089112DOI Listing
June 2021

Systemic Characterization of Novel Immune Cell Phenotypes in Recurrent Pregnancy Loss.

Front Immunol 2021 28;12:657552. Epub 2021 May 28.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Recurrent pregnancy loss (RPL) is a disturbing disease in women, and 50% of RPL is reported to be associated with immune dysfunction. Most previous studies of RPL focused mainly on the relationship between RPL and either T cells or natural killer (NK) cells in peripheral blood and the decidua; few studies presented the systemic profiles of the peripheral immune cell subsets in RPL women. Herein, we simultaneously detected 63 immune cell phenotypes in the peripheral blood from nonpregnant women (NPW), women with a history of normal pregnancy (NP) and women with a history of RPL (RPL) by multi-parameter flow cytometry. The results demonstrated that the percentages of naïve CD4 T cells, central memory CD4 T cells, naïve CD8 T cells, mature NK cells, Vδ1 T cells and the ratio of Vδ1 T cells/Vδ2 T cells were significantly higher in the RPL group than those in the NPW and NP groups, whereas the percentages of terminal differentiated CD4 T cells, effective memory CD4 T cells, immature NK cells and Vδ2 T cells were significantly lower in the RPL group than those in the NPW and NP groups. Interestingly, we found that peripheral T helper (T) cells were more abundant in the NPW group than in the NP and RPL groups. Moreover, the percentage of Vδ2PD-1 gamma-delta (γδ) T cells was extremely high, above the 95 percentile limit, in the NP group compared with the NPW and RPL groups, which has never been reported before. In addition, we also determined the 5 percentile lower limit and 95 percentile upper limit of the significantly changed immunological parameters based on the files of the NPW group. Taken together, this is the first study to simultaneously characterize the multiple immune cell subsets in the peripheral blood at a relatively large scale in RPL, which might provide a global readout of the immune status for clinicians to identify clinically-relevant immune disorders and guide them to make clear and individualized advice and treatment plans.
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http://dx.doi.org/10.3389/fimmu.2021.657552DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195235PMC
May 2021

Impact of short-term exposure to extreme temperatures on diabetes mellitus morbidity and mortality? A systematic review and meta-analysis.

Environ Sci Pollut Res Int 2021 Jun 8. Epub 2021 Jun 8.

Gansu Province Hospital Rehabilitation Center, 53 Dingxi Road, Chengguan District, Lanzhou, 730000, Gansu, China.

The relationship between diabetes mellitus and short-term exposure to extreme temperatures remains controversial. A systematic review and meta-analysis were performed to assess the association between extreme temperatures and diabetes mellitus morbidity and mortality. PubMed, Embase, the Cochrane Library, Web of Science, and the Cumulative Index to Nursing and Allied Health Literature (CINAHL) were searched since inception to January 1, 2019, and updated on November 17, 2020. The results were combined using random effects model and reported as relative risk (RR) with 95% confidence interval (CI). In total, 32 studies met the inclusion criteria. (1) Both heat and cold exposures have impact on diabetes. (2) For heat exposure, the subgroup analysis revealed that the effect on diabetes mortality (RR=1.139, 95% CI: 1.089-1.192) was higher than morbidity (RR=1.012, 95% CI: 1.004-1.019). (3) With the increase of definition threshold, the impact of heat exposure on diabetes rose. (4) A stronger association between heat exposure and diabetes was observed in the elderly (≥ 60 years old) (RR=1.040, 95% CI: 1.017-1.064). In conclusion, short-term exposure to both heat and cold temperatures has impact on diabetes. The elderly is the vulnerable population of diabetes exposure to heat temperature. Developing definitions of heatwaves at the regional level are suggested.
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http://dx.doi.org/10.1007/s11356-021-14568-0DOI Listing
June 2021

Highly ordered macroporous dual-element-doped carbon from metal-organic frameworks for catalyzing oxygen reduction.

Chem Sci 2020 Aug 11;11(35):9584-9592. Epub 2020 Aug 11.

School of Chemistry and Molecular Engineering, Shanghai Key Laboratory of Green Chemistry and Chemical Processes, East China Normal University Shanghai 200062 China.

Multiple heteroatom-doped carbons with 3D ordered macro/meso-microporous structures have not been realized by simple carbonization of metal-organic frameworks (MOFs). Herein, ordered macroporous phosphorus- and nitrogen-doped carbon (M-PNC) is prepared successfully by carbonization of double-solvent-induced MOF/polystyrene sphere (PS) precursors accompanied with spontaneous removal of the PS template, followed by post-doping. M-PNC shows a high specific surface area of 837 m g, nitrogen doping of 3.17 at%, and phosphorus doping of 1.12 at%. Thanks to the hierarchical structure, high specific surface area, and multiple heteroatom-doping, M-PNC exhibits unusual catalytic activity as an electrocatalyst for the oxygen reduction reaction. Computational calculation reveals that the P[double bond, length as m-dash]O group helps stabilize the adsorption of intermediates, and the position of P[double bond, length as m-dash]O relative to graphitic N significantly improves the activity of the adjacent carbons for electrocatalysis.
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http://dx.doi.org/10.1039/d0sc02518fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8162149PMC
August 2020

RNA atlas of human bacterial pathogens uncovers stress dynamics linked to infection.

Nat Commun 2021 06 2;12(1):3282. Epub 2021 Jun 2.

Department of Molecular Biology, Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden.

Bacterial processes necessary for adaption to stressful host environments are potential targets for new antimicrobials. Here, we report large-scale transcriptomic analyses of 32 human bacterial pathogens grown under 11 stress conditions mimicking human host environments. The potential relevance of the in vitro stress conditions and responses is supported by comparisons with available in vivo transcriptomes of clinically important pathogens. Calculation of a probability score enables comparative cross-microbial analyses of the stress responses, revealing common and unique regulatory responses to different stresses, as well as overlapping processes participating in different stress responses. We identify conserved and species-specific 'universal stress responders', that is, genes showing altered expression in multiple stress conditions. Non-coding RNAs are involved in a substantial proportion of the responses. The data are collected in a freely available, interactive online resource (PATHOgenex).
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http://dx.doi.org/10.1038/s41467-021-23588-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172932PMC
June 2021

DrugComb update: a more comprehensive drug sensitivity data repository and analysis portal.

Nucleic Acids Res 2021 Jun 1. Epub 2021 Jun 1.

Research Program in Systems Oncology, Faculty of Medicine, University of Helsinki, Helsinki FI-00290, Finland.

Combinatorial therapies that target multiple pathways have shown great promises for treating complex diseases. DrugComb (https://drugcomb.org/) is a web-based portal for the deposition and analysis of drug combination screening datasets. Since its first release, DrugComb has received continuous updates on the coverage of data resources, as well as on the functionality of the web server to improve the analysis, visualization and interpretation of drug combination screens. Here, we report significant updates of DrugComb, including: (i) manual curation and harmonization of more comprehensive drug combination and monotherapy screening data, not only for cancers but also for other diseases such as malaria and COVID-19; (ii) enhanced algorithms for assessing the sensitivity and synergy of drug combinations; (iii) network modelling tools to visualize the mechanisms of action of drugs or drug combinations for a given cancer sample and (iv) state-of-the-art machine learning models to predict drug combination sensitivity and synergy. These improvements have been provided with more user-friendly graphical interface and faster database infrastructure, which make DrugComb the most comprehensive web-based resources for the study of drug sensitivities for multiple diseases.
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http://dx.doi.org/10.1093/nar/gkab438DOI Listing
June 2021

Genomic divergence during artificial selection by feed conversion ratio in Pekin ducks.

Anim Biotechnol 2021 May 31:1-9. Epub 2021 May 31.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Pekin ducks are world-famous for its fast growth and have become the majority of breeds rearing in duck industry. Feed conversion ratio (FCR) is an important trait in Pekin ducks breeding and production, and the underlying biological processes are complex. To gain an insight to the possible biological mechanism underlying the FCR in Pekin ducks, an artificial selection population (S) and a natural population (Z7) were used in this study. The FCR of S line decreased from 2.184 ± 0.057 in the first generation to 1.886 ± 0.063 in the eighth generation, which displays significantly low FCR ( = 0.0032) than that of the Z7 line (2.23 ± 0.046). Then, 9 samples from eighth generation of S line and 10 samples from Z7 were used for whole-genome resequencing. Analyses of F, θπ and XP-EHH revealed 450, 479 and 356 candidate genes, which involved in 1,955, 1,933 and 1,964 candidate divergent regions (CDRs), respectively. And the integration of three approaches resulted in 30 overlapping genes. Functional analysis of 30 candidate genes revealed that variants of KCNQ1 and ADCY7, which were involved in the pancreatic secretion signal pathway, could be important molecular markers for high feed conversion efficiency in S line breeding.
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http://dx.doi.org/10.1080/10495398.2021.1927750DOI Listing
May 2021

Characterization of a sigma class GST (GSTS6) required for cellular detoxification and embryogenesis in Tribolium castaneum.

Insect Sci 2021 May 28. Epub 2021 May 28.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210046, China.

The sigma glutathione S-transferases (GSTSs) are a class of cytosolic glutathione S transferases (GSTs) that play important roles in antioxidant defense in insects, but the mechanisms by which GSTSs contribute to antioxidant activity remain unclear. Here, we isolated a GSTS (GSTS6) from Tribolium castaneum and explored its function. Homology and phylogenetic analysis revealed that TcGSTS6 shared high identity with other evolutionarily conserved GSTSs. The recombinant TcGSTS6 protein had strong activity toward cumene hydroperoxide and 4-hydroxynonenal but low activity toward the universal substrate 1-chloro-2,4-dinitrobenzene. Exposure to various types of oxidative stress, including heat, cold, UV and pathogenic microbes, significantly induced TcGSTs6 expression, which indicates that it is involved in antioxidant defense. Knockdown TcGSTs6 by using RNA interference (RNAi) caused reduced antioxidant capacity, which was accomplished by cooperating with other antioxidant genes. Moreover, treatment with various insecticides such as phoxim, lambda-cyhalothrin, dichlorvos and carbofuran revealed that TcGSTS6 plays an important role in insecticide detoxification. The RNAi results showed that TcGSTS6 is essential for embryogenesis in T. castaneum. Our study elucidates the mechanism by which a GSTS contributes to antioxidant activity and enhances our understanding of the functional diversity of GSTSs in insects.
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http://dx.doi.org/10.1111/1744-7917.12930DOI Listing
May 2021

Anti-LINGO-1 antibody ameliorates cognitive impairment, promotes adult hippocampal neurogenesis, and increases the abundance of CB1R-rich CCK-GABAergic interneurons in AD mice.

Neurobiol Dis 2021 May 24;156:105406. Epub 2021 May 24.

Department of Histology and Embryology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, PR China; Laboratory of Stem Cell and Tissue Engineering, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, PR China. Electronic address:

In view of the negative regulatory effect of leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 (LINGO-1) on neurons, an antibody against LINGO-1 (anti-LINGO-1 antibody) was herein administered to 10-month-old APP/PS1 transgenic Alzheimer's disease (AD) mice for 2 months as an experimental intervention. Behavioral, stereology, immunohistochemistry and immunofluorescence analyses revealed that the anti-LINGO-1 antibody significantly improved the cognitive abilities, promoted adult hippocampal neurogenesis (AHN), decreased the amyloid beta (Aβ) deposition, enlarged the hippocampal volume, and increased the numbers of total neurons and GABAergic interneurons, including GABAergic and CCK-GABAergic interneurons rich in cannabinoid type 1 receptor (CB1R), in the hippocampus of AD mice. In contrast, this intervention significantly reduced the number of GABAergic interneurons expressing LINGO-1 and CB1R in the hippocampus of AD mice. More importantly, we also found a negative correlation between LINGO-1 and CB1R on GABAergic interneurons in the hippocampus of AD mice, while the anti-LINGO-1 antibody reversed this relationship. These results indicated that LINGO-1 plays an important role in the process of hippocampal neuron loss in AD mice and that antagonizing LINGO-1 can effectively prevent hippocampal neuron loss and promote AHN. The improvement in cognitive abilities may be attributed to the improvement in AHN, and in the numbers of GABAergic interneurons and CCK-GABAergic interneurons rich in CB1Rs in the hippocampus of AD mice induced by the anti-LINGO-1 antibody. Collectively, the double target effect (LINGO-1 and CB1R) initiated by the anti-LINGO-1 antibody may provide an important basis for the study of drugs for the prevention and treatment of AD in the future.
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http://dx.doi.org/10.1016/j.nbd.2021.105406DOI Listing
May 2021

Advanced oxidation protein products impair autophagic flux in macrophage by inducing lysosomal dysfunction via activation of PI3K-Akt-mTOR pathway in Crohn's disease.

Free Radic Biol Med 2021 May 21;172:33-47. Epub 2021 May 21.

Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, China. Electronic address:

Dysfunction in macrophages is involved in the pathogenesis of various diseases, including Crohn's disease (CD). Previously, we found that advanced oxidation protein products (AOPPs) were predominantly deposited in macrophages in the intestinal lamina propria of CD patients. However, whether AOPPs contributes to macrophage dysfunction in CD and the underlying mechanism remains unknown. This study aimed to investigate the effects of AOPPs on macrophages functions in CD. In the present study, we discovered increased AOPPs levels were positively correlated with impaired autophagy in macrophages of CD patients. AOPPs could impair autophagic flux by inducing lysosomal dysfunction in RAW264.7 cell line and macrophages in AOPPs-treated mice, evidenced by increased number of autophagosomes, blocked degradation of autophagy-related proteins (LC3B-II and SQSTM1/p62), and decreased activity of lysosomal proteolytic enzymes after AOPPs challenge. Besides, AOPPs could also promote M1 polarization in RAW264.7 cells and bone marrow derived macrophages (BMDMs) in AOPPs-treated mice. In addition, our study revealed that PI3K-AKT-mTOR-TFEB pathway was activated by AOPPs in macrophages. Inhibition of the PI3K pathway effectively alleviated AOPPs-induced autophagy impairment and M1 polarization both in vitro and in vivo, thus reducing intestinal inflammation in AOPPs-challenged mice. Together, this study demonstrates that AOPPs-induced autophagy impairment in macrophages is crucial for CD progression.
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http://dx.doi.org/10.1016/j.freeradbiomed.2021.05.018DOI Listing
May 2021

Selection of the fusion and fixation range in the intervertebral surgery to correct thoracolumbar and lumbar tuberculosis: a retrospective clinical study.

BMC Musculoskelet Disord 2021 May 21;22(1):466. Epub 2021 May 21.

Department of Spine Surgery, General Hospital of Ningxia Medical University, 804 Shengli Street, Xingqing District, Yinchuan, 750004, People's Republic of China.

Background: To compare the diseased verses the non-diseased intervertebral surgery used in the treatment of thoracolumbar and lumbar spinal tuberculosis and to explore the best choice of fusion of fixation range.

Methods: Two hundred twenty-one patients with thoracolumbar and lumbar tuberculosis were categorized into two groups. One hundred eighteen patients underwent the diseased intervertebral surgery (lesion vertebral pedicle fixation, Group A) and 103 patients underwent the non-diseased intervertebral surgery (1 or 2 vertebral fixation above and below the affected vertebra, group B). Spinal tuberculosis diagnosis was confirmed in both groups of patients before lesion removal, bone graft fusion, and internal fixation. Clinical data and efficacy of the two surgical methods were then evaluated.

Results: The mean follow-up duration for both procedures was 65 months (50-68 months range). There were no significant differences in laboratory examinations, VAS scores, and the Cobb angle correction rate and the angle loss. However, significant differences existed in the operation time, blood loss, serosanguineous drainage volume, and blood transfusion requirement between the two groups. The diseased intervertebral surgery group performed significantly better than the non-diseased intervertebral surgery group in all of these areas. In both cases, the bone graft fused completely with the normal bone by the last follow-up, occuring at 50-86 months post surgery.

Conclusion: The diseased intervertebral surgery is a safe and feasible option for the treatment of thoracolumbar and lumbar tuberculosis. It effectively restores the physiological curvature of the spine and reduces the degeneration of adjacent vertebral bodies in the spinal column.
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http://dx.doi.org/10.1186/s12891-021-04335-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140488PMC
May 2021

Association Between Urinary Stress Incontinence and Levator Avulsion Detected by 3D Transperineal Ultrasound.

Ultraschall Med 2021 May 20. Epub 2021 May 20.

Gynecological pelvic floor and oncology, Chongqing Health Center for Women and Children, Chongqing, China.

Purpose:  To determine the association between levator avulsion and urinary stress incontinence (USI) by 3/4D transperineal ultrasound.

Materials And Methods:  842 patients who were admitted to our hospital from 2016 to 2019 were recruited for our study. 3D/4D transperineal ultrasound was performed. After standard interview and clinical evaluation, general conditions and levator hiatus data were collected and measured to compare with each group. The odds ratio (OR) of USI symptoms or ultrasound features with levator avulsion were calculated.

Results:  A total of 593 women were studied: 204 suffered from levator avulsion (96 cases of left-side avulsion, 80 cases of right-side avulsion and 28 cases of bilateral avulsion) and 389 women had no avulsion. The gravidity and episiotomy conditions of the avulsion groups were significantly different from the no-avulsion group. Significant differences were found in the transverse diameters and anteroposterior diameters between the levator avulsion group and the no-avulsion group, but there was no difference among the avulsion groups, regardless of whether the patient was at rest or performing the Valsalva maneuver. Interestingly, a significant difference was found in the presence of USI symptoms between the uni-avulsion group and the no-avulsion group. The odds ratio (OR) of USI symptoms in the uni-avulsion group is 2.786 (95 %CI, 1.663-4.669), but 0.939 (95 %CI, 0.276-3.199) for the bilateral avulsion and no-avulsion groups.

Conclusion:  Unilateral levator avulsion may be a risk factor for urinary stress incontinence.
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http://dx.doi.org/10.1055/a-1497-1838DOI Listing
May 2021

Genetic variations for egg internal quality of ducks revealed by genome-wide association study.

Anim Genet 2021 May 19. Epub 2021 May 19.

State Key Laboratory of Animal Nutrition; Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Egg internal quality traits are important traits related to egg production in poultry industry. To better understand the genetic architecture of egg internal quality traits in ducks, we performed genetic parameters estimates and a genome-wide association study (GWAS). The phenotypic values of egg weight, yolk color, albumin height (AH), yolk weight, and Haugh unit (HU) were collected individually from 352 F laying ducks produced by reciprocal crosses between mallards and Pekin ducks, and their genotypes were assayed by whole genome re-sequencing. The results showed that the AH and HU traits have a clear coefficient of variance, around 15% for both mallards and Pekin ducks. The pedigree-based genetic parameters estimates rane from 0.26 to 0.71 for all eight egg quality traits, while the highest heritability was 0.71 for egg weight. The GWAS showed that a clear signal was associated with AH and HU traits. The locus zoom analysis and conditional GWAS helped to narrow the candidate region to ~5.8-Mb spanning from 14.7 to 20.5 Mb on Chromosome 5, which harbored 111 candidate genes. MUC6 and LDLRAD3 were finally promised as the major candidate genes affecting albumen composition. Our data revealed the egg internal quality traits for the first time in ducks, which provides a theoretical basis and technological support for improving duck egg internal quality.
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http://dx.doi.org/10.1111/age.13063DOI Listing
May 2021

An overview of aflatoxin B1 biotransformation and aflatoxin M1 secretion in lactating dairy cows.

Anim Nutr 2021 Mar 5;7(1):42-48. Epub 2021 Jan 5.

State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

Milk is considered a perfect natural food for humans and animals. However, aflatoxin B1 (AFB1) contaminating the feeds fed to lactating dairy cows can introduce aflatoxin M1 (AFM1), the main toxic metabolite of aflatoxins into the milk, consequently posing a risk to human health. As a result of AFM1 monitoring in raw milk worldwide, it is evident that high AFM1 concentrations exist in raw milk in many countries. Thus, the incidence of AFM1 in milk from dairy cows should not be underestimated. To further optimize the intervention strategies, it is necessary to better understand the metabolism of AFB1 and its biotransformation into AFM1 and the specific secretion pathways in lactating dairy cows. The metabolism of AFB1 and its biotransformation into AFM1 in lactating dairy cows are drawn in this review. Furthermore, recent data provide evidence that in the mammary tissue of lactating dairy cows, aflatoxins significantly increase the activity of a protein, ATP-binding cassette super-family G member 2 (ABCG2), an efflux transporter known to facilitate the excretion of various xenobiotics and veterinary drugs into milk. Further research should focus on identifying and understanding the factors that affect the expression of ABCG2 in the mammary gland of cows.
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http://dx.doi.org/10.1016/j.aninu.2020.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110862PMC
March 2021

Single cell RNA sequencing for breast cancer: present and future.

Cell Death Discov 2021 May 14;7(1):104. Epub 2021 May 14.

Department of Pathology, Harbin Medical University, Harbin, 150081, China.

Breast cancer is one of the most common malignant tumors in women. It is a heterogeneous disease related to genetic and environmental factors. Presently, the treatment of breast cancer still faces challenges due to recurrence and metastasis. The emergence of single-cell RNA sequencing (scRNA-seq) technology has brought new strategies to deeply understand the biological behaviors of breast cancer. By analyzing cell phenotypes and transcriptome differences at the single-cell level, scRNA-seq reveals the heterogeneity, dynamic growth and differentiation process of cells. This review summarizes the application of scRNA-seq technology in breast cancer research, such as in studies on cell heterogeneity, cancer cell metastasis, drug resistance, and prognosis. scRNA-seq technology is of great significance to deeply analyze the mechanism of breast cancer occurrence and development, identify new therapeutic targets and develop new therapeutic approaches for breast cancer.
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http://dx.doi.org/10.1038/s41420-021-00485-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121804PMC
May 2021

Realizing a 14% single-leg thermoelectric efficiency in GeTe alloys.

Sci Adv 2021 May 7;7(19). Epub 2021 May 7.

Interdisciplinary Materials Research Center, School of Materials Science and Engineering, Tongji University, 4800 Caoan Rd., Shanghai 201804, China.

GeTe alloys have recently attracted wide attention as efficient thermoelectrics. In this work, a single-leg thermoelectric device with a conversion efficiency as high as 14% under a temperature gradient of 440 K was fabricated on the basis of GeTe-CuTe-PbSe alloys, which show a peak thermoelectric figure of merit () > 2.5 and an average of 1.8 within working temperatures. The high performance of the material is electronically attributed to the carrier concentration optimization and thermally due to the strengthened phonon scattering, the effects of which all originate from the defects in the alloys. A design of Ag/SnTe/GeTe contact successfully enables both a prevention of chemical diffusion and an interfacial contact resistivity of 8 microhm·cm for the realization of highly efficient devices with a good service stability/durability. Not only the material's high performance but also the device's high efficiency demonstrated the extraordinariness of GeTe alloys for efficient thermoelectric waste-heat recovery.
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http://dx.doi.org/10.1126/sciadv.abf2738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104868PMC
May 2021

Development of a Multivariable Prediction Model for Citrate Accumulation in Liver Transplant Patients Undergoing Continuous Renal Replacement Therapy with Regional Citrate Anticoagulation.

Blood Purif 2021 May 5:1-11. Epub 2021 May 5.

Surgical Intensive Care Unit, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Introduction: Patients with impaired citrate metabolism may experience citrate accumulation (CA), which causes life-threatening metabolic acidosis and hypocalcemia. CA poses a challenge for clinicians when deciding on the use of regional citrate anticoagulation (RCA) for patients with liver dysfunction. This study aimed to develop a prediction model integrating multiple clinical variables to assess the risk of CA in liver transplant patients.

Methods: This single-center prospective cohort study included postoperative liver transplant patients who underwent continuous renal replacement therapy (CRRT) with RCA. The study end point was CA. A prediction model was developed using a generalized linear mixed-effect model based on the Akaike information criterion. The predictive values were assessed using the receiver operating characteristic curve and bootstrap resampling (times = 500) to estimate the area under the curve (AUC) and the corresponding 95% confidence interval (CI). A nomogram was used to visualize the model.

Results: This study included 32 patients who underwent 133 CRRT sessions with RCA. CA occurred in 46 CRRT sessions. The model included lactate, norepinephrine >0.1 μg/kg/min, alanine aminotransferase, total bilirubin, and standard bicarbonate, which were tested before starting each CRRT session and body mass index, diabetes mellitus, and chronic kidney disease as predictors. The AUC of the model was 0.867 (95% CI 0.786-0.921), which was significantly higher than that of the single predictor (p < 0.05). A nomogram visualized the prediction model.

Conclusions: The prediction model integrating multiple clinical variables showed a good predictive value for CA. A nomogram visualized the model for easy application in clinical practice.
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http://dx.doi.org/10.1159/000513947DOI Listing
May 2021

Adipocyte-secreted microvesicle-derived miR-148a regulates adipogenic and osteogenic differentiation by targeting Wnt5a/Ror2 pathway.

Life Sci 2021 Aug 27;278:119548. Epub 2021 Apr 27.

Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, PR China. Electronic address:

Aims: Adipocyte-secreted microvesicles (MVs)-derived microRNAs (miRNAs) are relevant to adipogenic and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in osteonecrosis of the femoral head (ONFH). Our aims are to investigate the mechanism of adipocyte-derived MVs-miR-148a in ONFH.

Materials And Methods: Adipocyte-derived MVs were identified via transmission electron microscopy and specific markers expression. The adipogenic and osteogenic differentiation were investigated by Oil-Red O staining, alkaline phosphatase (ALP) activity, Alizarin Red S (ARS) staining and osteogenic or adipogenic factors levels. Genes and proteins expression were detected by using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. The relationship between miR-148a and Wnt5a was tested via dual-luciferase reporter analysis. The adipogenic differentiation and osteogenic differentiation in methylprednisolone (MPS)-induced ONFH rat model were assessed via hematoxylin-eosin (HE) staining, and immunohistochemical staining of collagen I (COL I).

Key Findings: Adipocyte-derived MVs promoted adipogenic differentiation via increasing Oil-Red O staining positive cells, adiponectin (Adipoq), acid-binding protein 2 (aP2) and peroxisome proliferator-activated receptor γ (PPAR-γ) levels, and repressed osteogenic differentiation of BMSCs via decreasing ARS staining positive cells, ALP, Runt-related transcription factor 2 (RUNX2) and osteocalcin (OCN) levels. MiR-148a was present in adipocyte-derived MVs, and miR-148a knockdown inhibited adipogenic differentiation and promoted osteogenic differentiation. Furthermore, Wnt5a expression was regulated by miR-148a. MiR-148a overexpression facilitated adipogenic differentiation and suppressed osteogenic differentiation via regulating the Wnt5a/Ror2 pathway. Adipocyte-derived MVs promoted adipogenic differentiation and inhibited osteogenic differentiation in MPS-induced ONFH rat model.

Significance: Adipocyte-derived MVs-miR-148a promoted adipogenic differentiation and suppressed osteogenic differentiation via targeting the Wnt5a/Ror2 pathway.
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http://dx.doi.org/10.1016/j.lfs.2021.119548DOI Listing
August 2021

The typically developing pediatric foot - The data of the 1744 children in China.

Foot Ankle Surg 2021 Apr 20. Epub 2021 Apr 20.

Department of Orthopedic Surgery, Chongqing Medical University First Affiliated Hospital, China. Electronic address:

Background: The medial longitudinal arch (MLA) improves with age in childhood. However, it still causes parents to worry that children have flat feet. Due to the lack of a standard to quantitatively assess the arch development in kids at certain age, the pediatricians judge the flat feet by experience, causing many cases to be overtreated. The aim of this study was to plot the distribution of MLA parameters in children.

Methods: Children without lower limb deformity and lower limb pain were recruited from 12 primary schools and kindergartens in Chongqing province-level city. Foot length (FL) and navicular height (NH) was measured manually, arch index (AI) and arch volume (AV) were measured with the Foot Plantar Scanner. Each parameter was measured in both weight-bearing and non-weight-bearing positions. Significant differences were also compared between the measurements of consecutive years.

Results: This study was the first to use a three-dimensional laser surface scanner to measure the MLA parameters of children aged 3-12 years in China. 1744 children (871 girls, 873 boys) participated in this study. FL, NH, AI and AV varied significantly with age in both the weight-bearing and non-weight-bearing positions. These parameters have significant differences between the weighted and non-weighted positions (p < 0.05).

Conclusions: The age distribution characteristics of these parameters indicated that the MLA improves with age. The establishment of a developmental scale for the children's MLA is necessary.
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http://dx.doi.org/10.1016/j.fas.2021.04.005DOI Listing
April 2021

Improved motor outcome prediction in Parkinson's disease applying deep learning to DaTscan SPECT images.

Comput Biol Med 2021 May 6;132:104312. Epub 2021 Mar 6.

Department of Electrical and Computer Engineering, Oakland University, Rochester, MI, USA; Department of Bioengineering, Oakland University, Rochester, MI, USA. Electronic address:

Purpose: Dopamine transporter (DAT) SPECT imaging is routinely used in the diagnosis of Parkinson's disease (PD). Our previous efforts demonstrated the use of DAT SPECT images in a data-driven manner by improving prediction of PD clinical assessment outcome using radiomic features. In this work, we develop a convolutional neural network (CNN) based technique to predict clinical motor function evaluation scores directly from longitudinal DAT SPECT images and non-imaging clinical measures.

Procedures: Data of 252 subjects from the Parkinson's Progression Markers Initiative (PPMI) database were used in this work. The motor part (III) score of the unified Parkinson's disease rating scale (UPDRS) at year 4 was selected as outcome, and the DAT SPECT images and UPDRS_III scores acquired at year 0 and year 1 were used as input data. The specified inputs and outputs were used to develop a CNN based regression method for prediction. Ten-fold cross-validation was used to test the trained network and the absolute difference between predicted and actual scores was used as the performance metric. Prediction using inputs with and without DAT images was evaluated.

Results: Using only UPDRS_III scores at year 0 and year 1, the prediction yielded an average difference of 7.6 ± 6.1 between the predicted and actual year 4 motor scores (range [5, 77]). The average difference was reduced to 6.0 ± 4.8 when longitudinal DAT SPECT images were included, which was determined to be statistically significant via a two-sample t-test, and demonstrates the benefit of including images.

Conclusions: This study shows that adding DAT SPECT images to UPDRS_III scores as inputs to deep-learning based prediction significantly improves the outcome. Without requiring segmentation and feature extraction, the CNN based prediction method allows easier and more universial application.
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http://dx.doi.org/10.1016/j.compbiomed.2021.104312DOI Listing
May 2021

Pharmacometabonomics: data processing and statistical analysis.

Brief Bioinform 2021 Apr 19. Epub 2021 Apr 19.

College of Pharmaceutical Sciences in Zhejiang University, China.

Individual variations in drug efficacy, side effects and adverse drug reactions are still challenging that cannot be ignored in drug research and development. The aim of pharmacometabonomics is to better understand the pharmacokinetic properties of drugs and monitor the drug effects on specific metabolic pathways. Here, we systematically reviewed the recent technological advances in pharmacometabonomics for better understanding the pathophysiological mechanisms of diseases as well as the metabolic effects of drugs on bodies. First, the advantages and disadvantages of all mainstream analytical techniques were compared. Second, many data processing strategies including filtering, missing value imputation, quality control-based correction, transformation, normalization together with the methods implemented in each step were discussed. Third, various feature selection and feature extraction algorithms commonly applied in pharmacometabonomics were described. Finally, the databases that facilitate current pharmacometabonomics were collected and discussed. All in all, this review provided guidance for researchers engaged in pharmacometabonomics and metabolomics, and it would promote the wide application of metabolomics in drug research and personalized medicine.
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http://dx.doi.org/10.1093/bib/bbab138DOI Listing
April 2021

Incidence, Risk Factors, and Attributable Mortality of Catheter-Related Bloodstream Infections in the Intensive Care Unit After Suspected Catheters Infection: A Retrospective 10-year Cohort Study.

Infect Dis Ther 2021 Jun 16;10(2):985-999. Epub 2021 Apr 16.

Department of Anesthesia, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

Introduction: Catheter management strategies for suspected catheter-related bloodstream infection (CRBSI) remain a major challenge in intensive care units (ICUs). The objective of this study was to determine the incidence, risk factors, and mortality attributable to CRBSIs in those patients.

Methods: A population-based surveillance on suspected CRBSI was conducted from 2009 to 2018 in a tertiary care hospital in China. We used the results of catheter tip culture to identify patients with suspected CRBSIs. Demographics, systemic inflammatory response syndrome (SIRS) criteria, interventions, and microorganism culture results were analysed and compared between patients with and without confirmed CRBSIs. Univariate and multivariate analyses identified the risk factors for CRBSIs, and attributable mortality was evaluated with a time-varying Cox proportional hazard model.

Results: In total, 686 patients with 795 episodes of suspected CRBSIs were included; 19.2% (153/795) episodes were confirmed as CRBSIs, and 17.4% (119/686) patients died within 30 days. The multifactor model shows that CRBSIs were associated with fever, hypotension, acute respiratory distress syndrome, hyperglycaemia and the use of continuous renal replacement therapy. The AUC was 77.0% (95% CI 73.3%-80.7%). The population attributable mortality fraction of CRBSI in patients was 18.2%, and mortality rate did not differ significantly between patients with and without CRBSIs (95% CI 0.464-1.279, P = 0.312).

Conclusions: This initial model based on the SIRS criteria is relatively better at identifying patients with CRBSI but only in domains of the sensitivity. There were no significant differences in attributable mortality due to CRBSI and other causes in patients with suspected CRBSI, which prompt catheter removal and re-insertion of new catheter may not benefit patients with suspected CRBSIs.

Trial Registration: China Clinical Trials Registration number; ChiCTR1900022175.
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http://dx.doi.org/10.1007/s40121-021-00429-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051286PMC
June 2021

Hippocampal PGC-1α-mediated positive effects on parvalbumin interneurons are required for the antidepressant effects of running exercise.

Transl Psychiatry 2021 Apr 15;11(1):222. Epub 2021 Apr 15.

Laboratory of Stem Cells and Tissue Engineering, Faculty of Basic Medical Sciences, Chongqing Medical University, 400016, Chongqing, People's Republic of China.

Running exercise was shown to have a positive effect on depressive-like symptoms in many studies, but the underlying mechanism of running exercise in the treatment of depression has not been determined. Parvalbumin-positive interneurons (PV interneurons), a main subtype of GABA neurons, were shown to be decreased in the brain during the depression. PGC-1α, a molecule that is strongly related to running exercise, was shown to regulate PV interneurons. In the present study, we found that running exercise increased the expression of PGC-1α in the hippocampus of depressed mice. Adult male mice with PGC-1α gene silencing in the hippocampus ran on a treadmill for 4 weeks. Then, depression-like behavior was evaluated by the behavioral tests, and the PV interneurons in the hippocampus were investigated. We found that running exercise could not improve depressive-like symptoms or increase the gene expression of PV because of the lack of PGC-1α in the hippocampus. Moreover, a lack of PGC-1α in the hippocampus decreased the number and activity of PV interneurons in the CA3 subfield of the hippocampus, and running exercise could not reverse the pathological changes because of the lack of PGC-1α. The present study demonstrated that running exercise regulates PV interneurons through PGC-1α in the hippocampus of mice to reverse depressive-like behaviors. These data indicated that hippocampal PGC-1α-mediated positive effects on parvalbumin interneurons are required for the antidepressant actions of running exercise. Our results will help elucidate the antidepressant mechanism of running exercise and identify new targets for antidepressant treatment.
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http://dx.doi.org/10.1038/s41398-021-01339-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050070PMC
April 2021

Atrophy of lacunosum moleculare layer is important for learning and memory in APP/PS1 transgenic mice.

Neuroreport 2021 May;32(7):596-602

Laboratory of Stem Cell and Tissue Engineering.

Changes in the hippocampus are closely associated with learning and memory in Alzheimer's disease; however, it is not clear which morphological and cellular and subcellular changes are essential for learning and memory. Here, we accurately quantitatively studied the hippocampal microstructure changes in Alzheimer's disease model mice and analyzed the relationship between the hippocampal microstructure changes and learning and memory. Ten-month-old male APP/PS1 transgenic mice and age-matched nontransgenic littermate mice were randomly selected. The spatial learning and memory abilities were assessed using the Morris water maze. The volumes of each layer and numbers of neurons, dendritic spines and oligodendrocytes in the hippocampal subregions were investigated using unbiased stereological techniques. The APP/PS1 transgenic mice showed a decline in hippocampus-dependent spatial learning and memory abilities, smaller volumes of each layer (other than stratum radiatum) and fewer numbers of neurons, dendritic spine synapses and mature oligodendrocytes in the hippocampal subregions than nontransgenic mice. In particular, the decline of spatial learning ability was significantly correlated with the atrophy of lacunosum moleculare layer (LMol) and the decrease of hippocampal neurons and mature oligodendrocytes rather than dendritic spines. The CA1-3 fields (including LMol) atrophy was significantly correlated with the decrease both of neurons, dendritic spines and mature oligodendrocytes. However, the dentate gyrus atrophy was significantly correlated with the decrease of neurons and mature oligodendrocytes rather than dendritic spines. The loss of neurons, dendritic spines synapses and mature oligodendrocytes together caused the LMol atrophy and then led to a decline in hippocampus-dependent spatial learning ability in mice with Alzheimer's disease.
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http://dx.doi.org/10.1097/WNR.0000000000001639DOI Listing
May 2021

Network-based modeling of herb combinations in traditional Chinese medicine.

Brief Bioinform 2021 Apr 8. Epub 2021 Apr 8.

Faculty of Medicine of the University of Helsinki and Group Leader of Network Pharmacology for Precision Medicine group, Finland.

Traditional Chinese medicine (TCM) has been practiced for thousands of years for treating human diseases. In comparison to modern medicine, one of the advantages of TCM is the principle of herb compatibility, known as TCM formulae. A TCM formula usually consists of multiple herbs to achieve the maximum treatment effects, where their interactions are believed to elicit the therapeutic effects. Despite being a fundamental component of TCM, the rationale of combining specific herb combinations remains unclear. In this study, we proposed a network-based method to quantify the interactions in herb pairs. We constructed a protein-protein interaction network for a given herb pair by retrieving the associated ingredients and protein targets, and determined multiple network-based distances including the closest, shortest, center, kernel, and separation, both at the ingredient and at the target levels. We found that the frequently used herb pairs tend to have shorter distances compared to random herb pairs, suggesting that a therapeutic herb pair is more likely to affect neighboring proteins in the human interactome. Furthermore, we found that the center distance determined at the ingredient level improves the discrimination of top-frequent herb pairs from random herb pairs, suggesting the rationale of considering the topologically important ingredients for inferring the mechanisms of action of TCM. Taken together, we have provided a network pharmacology framework to quantify the degree of herb interactions, which shall help explore the space of herb combinations more effectively to identify the synergistic compound interactions based on network topology.
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http://dx.doi.org/10.1093/bib/bbab106DOI Listing
April 2021

Drug synergy of combinatory treatment with remdesivir and the repurposed drugs fluoxetine and itraconazole effectively impairs SARS-CoV-2 infection in vitro.

Br J Pharmacol 2021 06 6;178(11):2339-2350. Epub 2021 Apr 6.

Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, and "Cells in Motion" Interfaculty Centre, University of Muenster, Muenster, Germany.

Background And Purpose: The SARS-COV-2 pandemic and the global spread of coronavirus disease 2019 (COVID-19) urgently call for efficient and safe antiviral treatment strategies. A straightforward approach to speed up drug development at lower costs is drug repurposing. Here, we investigated the therapeutic potential of targeting the interface of SARS CoV-2 with the host via repurposing of clinically licensed drugs and evaluated their use in combinatory treatments with virus- and host-directed drugs in vitro.

Experimental Approach: We tested the antiviral potential of the antifungal itraconazole and the antidepressant fluoxetine on the production of infectious SARS-CoV-2 particles in the polarized Calu-3 cell culture model and evaluated the added benefit of a combinatory use of these host-directed drugs with the direct acting antiviral remdesivir, an inhibitor of viral RNA polymerase.

Key Results: Drug treatments were well-tolerated and potently impaired viral replication. Importantly, both itraconazole-remdesivir and fluoxetine-remdesivir combinations inhibited the production of infectious SARS-CoV-2 particles > 90% and displayed synergistic effects, as determined in commonly used reference models for drug interaction.

Conclusion And Implications: Itraconazole-remdesivir and fluoxetine-remdesivir combinations are promising starting points for therapeutic options to control SARS-CoV-2 infection and severe progression of COVID-19.
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http://dx.doi.org/10.1111/bph.15418DOI Listing
June 2021

Volatile organic compound emission in tundra shrubs - Dependence on species characteristics and the near-surface environment.

Environ Exp Bot 2021 Apr;184:104387

Terrestrial Ecology Section, Department of Biology, University of Copenhagen, Universitetsparken 15, DK-2100, Copenhagen Ø, Denmark.

Temperature is one of the key abiotic factors during the life of plants, especially in the Arctic region which is currently experiencing rapid climate change. We evaluated plant traits and environmental variables determining leaf temperature in tundra shrubs and volatile organic compound (VOC) emissions with field measurements on deciduous tundra shrubs, and and evergreen and . Higher leaf-to-air temperature difference was observed in evergreen, compared to deciduous shrubs. Evergreen shrubs also showed continuously increasing photosynthesis with increasing temperature, suggesting high thermal tolerance. For the deciduous species, the optimum temperature for net photosynthesis was between our measurement temperatures of 24 °C and 38 °C. Air temperature and vapor pressure deficit were the most important variables influencing leaf temperature and VOC emissions in all the studied plants, along with stomatal density and specific leaf area in the deciduous shrubs. Using climate data and emission factors from our measurements, we modelled total seasonal tundra shrub VOC emissions of 0.3-2.3 g m over the main growing season. Our results showed higher-than-expected temperature optima for photosynthesis and VOC emission and demonstrated the relative importance of plant traits and local environments in determining leaf temperature and VOC emissions in a subarctic tundra.
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http://dx.doi.org/10.1016/j.envexpbot.2021.104387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896103PMC
April 2021

Four-decade dynamics of the water color in 61 large lakes on the Yangtze Plain and the impacts of reclaimed aquaculture zones.

Sci Total Environ 2021 Aug 24;781:146688. Epub 2021 Mar 24.

Department of Physical Geography and Ecosystem Science, Lund University, Sweden; Terrestrial Ecology Section, Department of Biology, University of Copenhagen, Copenhagen, Denmark.

The lakes on the Yangtze Plain, a critical source of freshwater and fisheries for hundreds of millions of people in China, have lost a considerable portion of their surface area due to reclamation since the 1950s. Landsat satellites can provide long-term collections of high-resolution images and thus offer great potential for hindcasting the lake reclamations of aquaculture zones and their long-term impacts on the lacustrine water color. Using Landsat observations from 1984 to 2018 and a Forel-Ule index (FUI) model, we studied the water color dynamics of 61 lakes on the Yangtze Plain. Three distinct change patterns were found among the 61 examined lakes, and 25 of the 61 lakes showed statistically significant changes in the annual hue angle values (P < 0.05). We further collected environmental parameter datasets (runoff, normalized difference vegetation index (NDVI), and wind speed) and a lacustrine reclamation dataset, and measured the concentrations of chlorophyll-a (Chl-a) and dissolved organic carbon (DOC) from two field trips. We investigated their correlations with water color change from different facets. The results showed that the long-term water color in 33 of the 61 lakes exhibited significant correlations with environmental factors. The reclaimed aquaculture zones in this region have caused differences in the water color between the reclaimed area and that in adjacent natural waters. The Chl-a and DOC levels derived from field surveys further confirmed that reclaimed aquaculture zones increased light-absorbing materials in the water and may deteriorate water quality. This study is an important step forward in understanding the water quality changes in lake ecosystems affected by human impacts and natural variability.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146688DOI Listing
August 2021

Progression and application of CRISPR-Cas genomic editors.

Methods 2021 Mar 25. Epub 2021 Mar 25.

Basic Medical School, Ningxia Medical University, Yinchuan, People's Republic of China. Electronic address:

Base editing technology is an efficient tool for genome editing, particularly in the correction of base mutations. Diverse base editing systems were developed according to the dCas9 or nCas9 linked with different deaminase or reverse transcriptase in the editors, including ABEs, CBEs, PEs and dual-functional of base editor (such as CGBE1, A&C-BEmax, ACBE, etc.). Currently, Base editing technology has been widely applied to various fields such as microorganisms, plants, animals and medicine for basic research and therapeutics. Here, we reviewed the advancement of base editing technology. We also discussed the application of base editors in different areas in the future.
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http://dx.doi.org/10.1016/j.ymeth.2021.03.013DOI Listing
March 2021