Publications by authors named "Jing Shen"

588 Publications

Comment on an article by Guido Garavaglia et al.: Short stem total hip arthroplasty with the direct anterior approach demonstrates suboptimal fixation.

Int Orthop 2021 Feb 24. Epub 2021 Feb 24.

Department of Orthopaedics, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Shangcheng District, Zhejiang, 310006, Hangzhou, China.

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http://dx.doi.org/10.1007/s00264-021-04989-4DOI Listing
February 2021

High Expression of Interleukin-2 Receptor Subunit Gamma Reveals Poor Prognosis in Human Gastric Cancer.

J Oncol 2021 21;2021:6670834. Epub 2021 Jan 21.

Key Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, and the Department of Physiology, Shanxi Medical University, Taiyuan, China.

Precision medicine for gastric cancer (GC) is still an unsolved issue, because most available target drugs are not specifically designed for GC. Exploring novel signaling molecules with target value for GC is in urgent need. This study aimed to reveal that interleukin-2 receptor subunit gamma (IL2RG) is such a key molecule in human GC progression. GC tissues and paracancerous gastric tissues were collected from 7 patients (5 males and 2 females) during tumor radical excision surgery. These tissues were used to identify the differentially expressed genes (DEGs) with RNA-seq and serial bioinformatics analyses including Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, gene expression profiling interactive analysis (GEPIA), and survival analysis. RT-qPCR and western blotting were performed to compare the mRNA and protein expression levels of IL2RG between GC tissues and adjacent normal gastric tissues as well as between GC cell line SGC-7901 and normal gastric epithelial cell line GES-1. Results showed striking elevations of IL2RG both in the mRNA and protein levels in GC tissues and human gastric cancer SGC-7901 cell line compared, respectively, with the adjacent normal gastric tissues and normal GES-1 cells, and higher IL2RG expression was associated with lower survival. Analyses on the GSE29272 and GSE15459 datasets from Gene Expression Omnibus verified that IL2RG was highly expressed in GC patients and was associated with poor overall survival. In addition, molecular docking showed that a small molecule, resatorvid (TAK 242), might be an inhibitor of IL2RG. We conclude that IL2RG is overexpressed in advanced GC and is associated with low survival. IL2RG may serve as a biomarker of GC progression and poor prognosis.
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http://dx.doi.org/10.1155/2021/6670834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843183PMC
January 2021

A novel deodorization method of edible oil by using ethanol steam at low temperature.

J Food Sci 2021 Feb 18;86(2):394-403. Epub 2021 Jan 18.

Institute of Agricultural Quality Standards and Testing Technology Research, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China.

A novel deodorization method of edible oil by using ethanol steam at low-temperature was developed. We compared the chemical changes in predeodorized rapeseed oil after anhydrous ethanol steam distillation at low temperature (140 to 220 °C) (L-ESD) and conventional high-temperature (250 °C) water-steam distillation (H-WSD) in terms of odor characteristics, physicochemical properties, micronutrient contents, antioxidant performance, and fatty acid composition. Compared with H-WSD (250 °C for 60 min), L-ESD at 180 °C for 80 to 100 min resulted in lower response values of electronic nose, free fatty acid (0.03% to 0.07%), and peroxide value (0.00 to 0.67 meq/kg), but higher retention of tocopherols (554.93 to 551.59 mg/kg), total phenols (43.36 to 45.42 mgGAE/kg), total carotenoids (65.78 to 67.85 mg/kg), phytosterols (585.80 to 596.53 mg/100 g), polyunsaturated fatty acids (27.95 to 28.01%), and better antioxidant properties. In conclusion, L-ESD can mitigate the damage of oil and thus significantly improve the safety of vegetable oils with a high retention of nutrients compared with conventional H-WSD. PRACTICAL APPLICATION: The present study aimed to compare the chemical changes in predeodorized rapeseed oil after anhydrous ethanol steam distillation at low temperature (140 to 220 °C) (L-ESD) and conventional high-temperature (250 °C) water-steam distillation (H-WSD) in terms of odor characteristics, physicochemical properties, micronutrient contents, antioxidant performance, and fatty acid composition. Results indicated that this finding supplies a theoretical basis for developing a method with retaining more micronutrients and producing less harmful substances for the deodorization of rapeseed oil.
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http://dx.doi.org/10.1111/1750-3841.15578DOI Listing
February 2021

Correction: Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer.

Oncotarget 2020 Dec 29;11(52):4845-4846. Epub 2020 Dec 29.

The Sixth Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, China.

[This corrects the article DOI: 10.18632/oncotarget.21068.].
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http://dx.doi.org/10.18632/oncotarget.27740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779255PMC
December 2020

Distribution and Dynamic Changes in Matrix Metalloproteinase (MMP)-2, MMP-9, and Collagen in an In Stent Restenosis Process.

Eur J Vasc Endovasc Surg 2021 Jan 10. Epub 2021 Jan 10.

Department of Intervention, Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address:

Objective: The aim of this study was to observe the spatial distribution and dynamic changes of matrix metalloproteinase (MMP)-2, MMP-9, and collagen in in stent restenosis (ISR) and to explore their influence on ISR.

Methods: Sixty Z type stents were implanted into the common iliac arteries of minipigs, which were divided into 10 groups (six in each group) according to euthanasia time (6 hours, and 1, 3, 7, 14, 28, 56, 84, 168, and 336 days). After the samples were harvested, haematoxylin and eosin staining, immunohistochemical staining, Western blotting, and Picrosirius red staining were performed for all groups.

Results: ISR occurred in all six minipigs in the 56 day group (percentage diameter stenosis range 71.6%-79.2%, mean ± standard deviation 75.6% ± 2.5%). The percentage diameter stenosis decreased to 38.3% ± 2.7% at 336 days (p < .001). Immunohistochemical staining showed that MMP-2 and MMP-9 were strongly stained near the internal elastic lamina or in the damaged parts of the intima, around the struts and neointimal lumen surface in the ISR process. The expression of MMP-2 and MMP-9 at 56 days was significantly lower compared with their peaks (seven days and one day [p < .001; p = .002], respectively). At 56 days, the collagen content reached its maximum (mean integrated optical density range 0.73-0.92, mean ± standard deviation 0.82 ± 0.09). From the 14 day group to the 336 day group, mature collagen in neointima was correlated negatively with MMP-2 (γ(36) = -0.816; p < .001) and MMP-9 expression (γ(36) = -0.853; p < .001). During the neointimal regression period, new collagen in neointima was positively correlated with MMP-2 (γ(24) = 0.683; p < .001) and MMP-9 (γ(24) = 0.873; p < .001).

Conclusion: This study has demonstrated the spatial distribution of and dynamic changes in MMP-2, MMP-9, and collagen in ISR by simulating the process of neointima from generation to regression after stent implantation. When ISR occurred, MMP-2 and MMP-9 expression decreased and collagen content reached its maximum, which might contribute to ISR.
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http://dx.doi.org/10.1016/j.ejvs.2020.11.035DOI Listing
January 2021

Older Listeners' Perception of Speech With Strengthened and Weakened Dynamic Pitch Cues in Background Noise.

Authors:
Jing Shen

J Speech Lang Hear Res 2021 Feb 13;64(2):348-358. Epub 2021 Jan 13.

Department of Speech, Language and Hearing Sciences, Western Michigan University, Kalamazoo.

Purpose Dynamic pitch, which is defined as the variation in fundamental frequency, is an acoustic cue that aids speech perception in noise. This study examined the effects of strengthened and weakened dynamic pitch cues on older listeners' speech perception in noise, as well as how these effects were modulated by individual factors including spectral perception ability. Method The experiment measured speech reception thresholds in noise in both younger listeners with normal hearing and older listeners whose hearing status ranged from near-normal hearing to mild-to-moderate sensorineural hearing loss. The pitch contours of the target speech were manipulated to create four levels of dynamic pitch strength: weakened, original, mildly strengthened, and strengthened. Listeners' spectral perception ability was measured using tests of spectral ripple and frequency modulation discrimination. Results Both younger and older listeners performed worse with manipulated dynamic pitch cues than with original dynamic pitch. The effects of dynamic pitch on older listeners' speech recognition were associated with their age but not with their perception of spectral information. Those older listeners who were relatively younger were more negatively affected by dynamic pitch manipulations. Conclusions The findings suggest the current pitch manipulation strategy is detrimental for older listeners to perceive speech in noise, as compared to original dynamic pitch. While the influence of age on the effects of dynamic pitch is likely due to age-related declines in pitch perception, the spectral measures used in this study were not strong predictors for dynamic pitch effects. Taken together, these results indicate next steps in this line of work should be focused on how to manipulate acoustic cues in speech in order to improve speech perception in noise for older listeners.
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http://dx.doi.org/10.1044/2020_JSLHR-20-00116DOI Listing
February 2021

Comment on article by Milenkovic et al.: lateral tibial plateau fractures-functional outcomes and complications after open reduction and internal fixation.

Int Orthop 2021 Jan 8. Epub 2021 Jan 8.

Department of Orthopaedics, The First Affiliated Hospital of Zhejiang Chinese Medical University, 54 Youdian Road, Shangcheng District, Hangzhou, 310006, Zhejiang, China.

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http://dx.doi.org/10.1007/s00264-020-04893-3DOI Listing
January 2021

Long Non-Coding RNAs: Potential Biomarkers and Targets for Hepatocellular Carcinoma Therapy and Diagnosis.

Int J Biol Sci 2021 1;17(1):220-235. Epub 2021 Jan 1.

Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming, Yunnan, China.

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. Increasing studies showed that long non-coding RNAs (lncRNAs), a novel class of RNAs that are greater than 200 nucleotides in length but lack the ability to encode proteins, exert crucial roles in the occurrence and progression of HCC. LncRNAs promote the proliferation, migration, invasion, autophagy, and apoptosis of tumor cells by regulating downstream target gene expression and cancer-related signaling pathways. Meanwhile, lncRNA can be used as biomarkers to predict the efficacy of HCC treatment strategies, such as surgery, radiotherapy, chemotherapy, and immunotherapy, and as a potential individualized tool for HCC diagnosis and treatment. In this review, we overview up-to-date findings on lncRNAs as potential biomarkers for HCC surgery, radiotherapy, chemotherapy resistance, target therapy, and immunotherapy, and discuss the potential clinical application of lncRNA as tools for HCC diagnosis and treatment.
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http://dx.doi.org/10.7150/ijbs.50730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757045PMC
January 2021

mC RNA Methylation Primarily Affects the ErbB and PI3K-Akt Signaling Pathways in Gastrointestinal Cancer.

Front Mol Biosci 2020 7;7:599340. Epub 2020 Dec 7.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, China.

5-Methylcytosine (mC) is a kind of methylation modification that occurs in both DNA and RNA and is present in the highly abundant tRNA and rRNA. It has an important impact on various human diseases including cancer. The function of mC is modulated by regulatory proteins, including methyltransferases (writers) and special binding proteins (readers). This study aims at comprehensive study of the mC RNA methylation-related genes and the main pathways under mC RNA methylation in gastrointestinal (GI) cancer. Our result showed that the expression of mC writers and reader was mostly up-regulated in GI cancer. The gene has the highest proportion of mutations found in GI cancer. Importantly, in liver cancer, higher expression of almost all mC regulators was significantly associated with lower patient survival rate. In addition, the expression level of mC-related genes is significantly different at various pathological stages. Finally, we have found through bioinformatics analysis that mC regulatory proteins are closely related to the ErbB/PI3K-Akt signaling pathway and was an important target for mC regulators. Besides, the compound termed streptozotocin may be a key candidate drug targeting on GSK3B for molecular targeted therapy in GI cancer.
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http://dx.doi.org/10.3389/fmolb.2020.599340DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750483PMC
December 2020

Comprehensive assessment of PD-L1 and PD-L2 dysregulation in gastrointestinal cancers.

Epigenomics 2020 Dec 18;12(24):2155-2171. Epub 2020 Dec 18.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, PR China.

and are ligands of . Their overexpression has been reported in different cancers. However, the underlying mechanism of and dysregulation and their related signaling pathways are still unclear in gastrointestinal cancers. The expression of and were studied in The Cancer Genome Atlas and Genotype-Tissue Expression databases. The gene and protein alteration of and were analyzed in cBioportal. The direct transcription factor regulating / was determined with ChIP-seq data. The association of expression with clinicopathological parameters, survival, immune infiltration and tumor mutation burden were investigated with data from The Cancer Genome Atlas. Potential targets and pathways of and were determined by protein enrichment, WebGestalt and gene ontology. Comprehensive analysis revealed that and were significantly upregulated in most types of gastrointestinal cancers and their expressions were positively correlated. was a key transcription factor regulating the expression of . Higher or expression was significantly associated with poor overall survival, higher tumor mutation burden and more immune and stromal cell populations. Finally, HIF-1, ERBB and mTOR signaling pathways were most significantly affected by and dysregulation. Altogether, this study provided comprehensive analysis of the dysregulation of and , its underlying mechanism and downstream pathways, which add to the knowledge of manipulating for cancer immunotherapy.
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http://dx.doi.org/10.2217/epi-2020-0093DOI Listing
December 2020

Genotyping and Molecular Characterization of Fluoroquinolone's Resistance Among Multidrug-Resistant in Southwest of China.

Microb Drug Resist 2020 Dec 10. Epub 2020 Dec 10.

Tuberculosis Reference Laboratory, Chongqing Tuberculosis Control Institute, Chongqing, China.

Although fluoroquinolones (FQs) are the backbone drugs for the treatment of multidrug-resistant tuberculosis (MDR-TB), the knowledge about the resistance pattern and molecular characterization of new-generation FQs in Chongqing is limited. This study aimed to investigate the resistance rate and mutation types of later-generation FQs against MDR-TB in Chongqing, and further to explore the relationship between different genotypes and phenotypes. A total of 967 clinical strains were characterized using multilocus sequence typing and drug susceptibility testing, followed by analysis of genotype/phenotype association. The 229 (23.7%, 229/967) isolates were identified as MDR-TB. The most effective agent against MDR-TB was gatifloxacin (GFX) (20.1%, 46/229), and the highest resistant rate was observed in ofloxacin (OFX) (41.0%, 94/229). Of the 190 strains (83.0%) identified as Beijing genotype, 111 isolates were modern Beijing genotype (58.4%) and 79 isolates were ancient Beijing genotype (41.6%). By analyzing 94 OFX-resistant isolates, 13 isolates were clustered with the cumulative clustering rate of 13.8% (13/94). Of the 91 isolates (39.7%, 91/229) with a mutation in gene, mutation in codon 94 was the most prevalent. Only 15 isolates (6.6%, 15/229) harbored a mutation in gene. There was no significant difference in the mutation rate of gene between Beijing and non-Beijing genotype, clustered isolates, and nonclustered isolates ( > 0.05).
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http://dx.doi.org/10.1089/mdr.2019.0339DOI Listing
December 2020

The mental health of Chinese healthcare staff in non-epicenter of COVID-19: a cross-sectional study.

Ann Palliat Med 2020 Nov;9(6):4127-4136

Department of Pediatric Intensive Care Unit, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Background: The COVID-19 is a public health issue all over the world. It is an unprecedented challenge for society. Frontline medical staff are at high risk of mental health problems due to the overwhelming workload, worry of infection, and inadequate protective instruments. The study is to investigate the psychological status of medical staff in a women and children's hospital in non-epicenter of COVID-19 during the early stage of the COVID-19 outbreak.

Methods: A total of 2,143 hospital medical staff participated in a cross-sectional online survey. Psychological response levels were assessed using the Psychological Questionnaire on Emergency Events in Public Health (PQEEPH), and mental health status was measured using the Generalized Anxiety Disorder-7 (GAD-7) scale.

Results: Eligible responses were received from 1,890 (90.6%) women and 197 (9.4%) men. In total, 10.3% of respondents rated the psychological impact of the outbreak as moderate or severe, and 4% reported severe anxiety symptoms.

Conclusions: Our findings indicated that the majority of the medical staff are in great level of mental health. The study demonstrates that females, the 31-40 age group, and those who have been widowed experienced greater levels of anxiety and therefore require more support. Furthermore, the cognition of medical workers might affect their emotional reactions. Measures to decrease anxiety during emergency outbreaks should therefore include communication strategies.
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http://dx.doi.org/10.21037/apm-20-2041DOI Listing
November 2020

Multiple failures of internal fixation for treatment of periprosthetic femoral refracture: a case report and literature review.

J Int Med Res 2020 Dec;48(12):300060520958972

Orthopedics Department, The First Affiliated Hospital of Zhejiang Chinese Medical University, HangZhou, ZheJiang, P.R. China.

We herein report a case involving three failures of internal fixation after periprosthetic femoral fracture (Vancouver type B1). The patient had low bone mass (T-score of -1.7) and was overweight (body mass index of 28.7 kg/m) but had no sign of femoral stem loosening. The first open reduction with internal fixation was performed according to the recommended treatment. Unexpectedly, three treatment failures subsequently occurred, after which the patient finally attained endurable walking activity. A literature review indicated that the intrinsic biomechanical problems of Vancouver B1 fractures have not been thoroughly addressed. Choosing the correct surgical strategy for Vancouver B1 fractures is essential to avoid complications and ensure healing. A sufficient locking plate and cable system should be used after the first failure if revision was not performed the first time.
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http://dx.doi.org/10.1177/0300060520958972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727066PMC
December 2020

[Efficacy and safety of tranexamic acid in total hip arthroplasty via direct anterior approach].

Zhongguo Gu Shang 2020 Nov;33(11):1037-41

Department of Orthopaedics, Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China.

Objective: To evaluate the efficacy and safety of local application of tranexamic acid (TXA) in reducing perioperative blood loss in total hip arthroplasty via direct anterior approach (DAA).

Methods: From July 2013 to September 2018, 46 patients with avascular necrosis of the femoral head were divided into tranexamic acid group (=23) and saline group (=23). In the tranexamic acid group, there were 14 males and 9 females, aged 52 to 72(63.70±5.34) years old. They were diluted with 3 g tranexamic acid in 50 ml normal saline and immersed in the joint cavity for 3 min after prosthesis replacement;in the normal saline group, there were 13 males and 10 females, aged 55 to 73 (61.26±5.78) years, who were treated with the sameamount of normal saline. The blood loss, hemoglobin value, number of blood transfusion cases, the time of first landing after operation, the incidence of thrombosis and incision adverse events were compared between the two groups. Harris score was used to evaluate hip joint function at 1 and 3 months after operation.

Results: The incision healed well and no obvious complications occurred in the two groups. All patients were followed up for 12 to 59 months(averaged 31.11 months). No hip pain was found in the follow-up patients. Hip joint function was improved effectively and no prosthesis loosening occurred. The total perioperative blood loss in tranexamic acid group and normal saline group was(740.09±77.14) ml and (1 069.07±113.53) ml respectively, 24 hours after operation, the drainage volume was (87.61±9.28) ml, (233.83±25.62) ml, the hidden blood loss was (409.65±38.01) ml and (588.33±57.16) ml. the difference of hemoglobin before and after operation was (24.78±2.19) g / L and (33.57±2.95) g / L, the difference was statistically significant (<0.05). There was no significant difference in blood loss, incidence of deep vein thrombosis and pulmonary embolism, and Harris score of hip joint between the two groups (>0.05).

Conclusion: local application of tranexamic acid in total hip arthroplasty through direct anterior approach can safely and effectively reduce perioperative blood loss, and does not increase the risk of thrombosis, and does not affect the normal recovery of joint function.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2020.11.010DOI Listing
November 2020

hGC33-Modified and Sorafenib-Loaded Nanoparticles have a Synergistic Anti-Hepatoma Effect by Inhibiting Wnt Signaling Pathway.

Nanoscale Res Lett 2020 Nov 26;15(1):220. Epub 2020 Nov 26.

Wuhu Research Institute, Anhui University of Science and Technology, Huainan, 232001, China.

Delivery of tumor-specific inhibitors is a challenge in cancer treatment. Antibody-modified nanoparticles can deliver their loaded drugs to tumor cells that overexpress specific tumor-associated antigens. Here, we constructed sorafenib-loaded polyethylene glycol-b-PLGA polymer nanoparticles modified with antibody hGC33 to glypican-3 (GPC3 +), a membrane protein overexpressed in hepatocellular carcinoma. We found that hGC33-modified NPs (hGC33-SFB-NP) targeted GPC3 hepatocellular carcinoma (HCC) cells by specifically binding to GPC3 on the surface of HCC cells, inhibited Wnt-induced signal transduction, and inhibited HCC cells in G0/1 by down-regulating cyclin D1 expression, thus attenuating HCC cell migration by inhibiting epithelial-mesenchymal transition. hGC33-SFB-NP inhibited the migration, cycle progression, and proliferation of HCC cells by inhibiting the Ras/Raf/MAPK pathway and the Wnt pathway in tandem with GPC3 molecules, respectively. hGC33-SFB-NP inhibited the growth of liver cancer in vivo and improved the survival rate of tumor-bearing mice. We conclude that hGC33 increases the targeting of SFB-NP to HCC cells. hGC33-SFB-NP synergistically inhibits the progression of HCC by blocking the Wnt pathway and the Ras/Raf/MAPK pathway.
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http://dx.doi.org/10.1186/s11671-020-03451-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691417PMC
November 2020

Open urethroplasty versus endoscopic urethrotomy for recurrent urethral stricture in men: the OPEN RCT.

Health Technol Assess 2020 11;24(61):1-110

St George's University Hospitals NHS Foundation Trust, London, UK.

Background: Men who suffer recurrence of bulbar urethral stricture have to decide between endoscopic urethrotomy and open urethroplasty to manage their urinary symptoms. Evidence of relative clinical effectiveness and cost-effectiveness is lacking.

Objectives: To assess benefit, harms and cost-effectiveness of open urethroplasty compared with endoscopic urethrotomy as treatment for recurrent urethral stricture in men.

Design: Parallel-group, open-label, patient-randomised trial of allocated intervention with 6-monthly follow-ups over 24 months. Target sample size was 210 participants providing outcome data. Participants, clinicians and local research staff could not be blinded to allocation. Central trial staff were blinded when needed.

Setting: UK NHS with recruitment from 38 hospital sites.

Participants: A total of 222 men requiring operative treatment for recurrence of bulbar urethral stricture who had received at least one previous intervention for stricture.

Interventions: A centralised randomisation system using random blocks allocated participants 1 : 1 to open urethroplasty (experimental group) or endoscopic urethrotomy (control group).

Main Outcome Measures: The primary clinical outcome was control of urinary symptoms. Cost-effectiveness was assessed by cost per quality-adjusted life-year (QALY) gained over 24 months. The main secondary outcome was the need for reintervention for stricture recurrence.

Results: The mean difference in the area under the curve of repeated measurement of voiding symptoms scored from 0 (no symptoms) to 24 (severe symptoms) between the two groups was -0.36 [95% confidence interval (CI) -1.78 to 1.02;  = 0.6]. Mean voiding symptom scores improved between baseline and 24 months after randomisation from 13.4 [standard deviation (SD) 4.5] to 6 (SD 5.5) for urethroplasty group and from 13.2 (SD 4.7) to 6.4 (SD 5.3) for urethrotomy. Reintervention was less frequent and occurred earlier in the urethroplasty group (hazard ratio 0.52, 95% CI 0.31 to 0.89;  = 0.02). There were two postoperative complications requiring reinterventions in the group that received urethroplasty and five, including one death from pulmonary embolism, in the group that received urethrotomy. Over 24 months, urethroplasty cost on average more than urethrotomy (cost difference £2148, 95% CI £689 to £3606) and resulted in a similar number of QALYs (QALY difference -0.01, 95% CI -0.17 to 0.14). Therefore, based on current evidence, urethrotomy is considered to be cost-effective.

Limitations: We were able to include only 69 (63%) of the 109 men allocated to urethroplasty and 90 (80%) of the 113 men allocated to urethrotomy in the primary complete-case intention-to-treat analysis.

Conclusions: The similar magnitude of symptom improvement seen for the two procedures over 24 months of follow-up shows that both provide effective symptom control. The lower likelihood of further intervention favours urethroplasty, but this had a higher cost over the 24 months of follow-up and was unlikely to be considered cost-effective.

Future Work: Formulate methods to incorporate short-term disutility data into cost-effectiveness analysis. Survey pathways of care for men with urethral stricture, including the use of enhanced recovery after urethroplasty. Establish a pragmatic follow-up schedule to allow national audit of outcomes following urethral surgery with linkage to NHS Hospital Episode Statistics.

Trial Registration: Current Controlled Trials ISRCTN98009168.

Funding: This project was funded by the NIHR Health Technology Assessment programme and will be published in full in ; Vol. 24, No. 61. See the NIHR Journals Library website for further project information.
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http://dx.doi.org/10.3310/hta24610DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750862PMC
November 2020

Comparative efficacy of seven exercise interventions for symptoms of depression in college students: A network of meta-analysis.

Medicine (Baltimore) 2020 Nov;99(47):e23058

Institute of natural resources and conservation lands, Hunan Academy of Forestry.

Background: Depression among college students is common, exercise interventions are valued as one of the most widely prescribed interventions for depressed college students, however, it is especially difficult for university administrators to determine which exercise intervention is most effective, and efficacy of exercise interventions among depressed college students have not been evaluated.

Objectives: To systematically review and compare the efficacy of 7 exercise interventions for decrease symptoms of depression in college students.

Method: A network of meta-analysis (NMA) was conducted to fill the objectives. Five relatived electronic databases were searched for the related articles.

Eligibility Criteria: Randomized controlled trials comparing the efficacy of 7 Exercise interventions with usual care of college students with depression were included in the review.

Main Outcomes: The primary outcome of the present study was standardized mean difference (SMD) and the mean change of depressive symptoms.

Results: Fourteentrials were identified, including 2010 depressed college students. The result of direct meta-analysis of this review indicated exercise interventions overall had a significantly lower mean depression scores (SMD = -1.13) when compared with usual care. The result of NMA indicated when comparing with badminton intervention, yoga (SMD = -7.7, 95%CI: -14 to -0.93) and Tai chi (SMD = -9.4, 95%CI: -16 to -2.7) can significantly decrease depression scores of the depressed college students. The rank of 7 exercise interventions with respect to efficiently decrease symptom of depressed undergraduates was Tai chi > Yoga > Volleyball > Dance > Run > Basketball > Badminton, respectively.

Conclusions: Tai chi exhibited the highest probability that became the most efficacy intervention among the comparions, and Yoga showed the second most effectiveness to alleviate depressive symptoms of depressed college students, and dance ranks the third, followed by run, volleyball, basketball, and badminton respectively.
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http://dx.doi.org/10.1097/MD.0000000000023058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7676569PMC
November 2020

Membrane nanotubes facilitate the propagation of inflammatory injury in the heart upon overactivation of the β-adrenergic receptor.

Cell Death Dis 2020 11 7;11(11):958. Epub 2020 Nov 7.

Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing Key Laboratory of Cardiovascular Receptors Research, 100191, Beijing, China.

Acute sympathetic stress quickly induces cardiac inflammation and injury, suggesting that pathogenic signals rapidly spread among cardiac cells and that cell-to-cell communication may play an important role in the subsequent cardiac injury. However, the underlying mechanism of this response is unknown. Our previous study demonstrated that acute β-adrenergic receptor (β-AR) signaling activates inflammasomes in the heart, which triggers the inflammatory cascade. In the present study, β-AR overactivation induced inflammasome activation in both the cardiomyocytes and cardiac fibroblasts (CFs) of mice hearts following a subcutaneous injection of isoproterenol (ISO, 5 mg/kg body weight), a selective agonist of β-AR. In isolated cardiac cells, ISO treatment only activated the inflammasomes in the cardiomyocytes but not the CFs. These results demonstrated that inflammasome activation was propagated from cardiomyocytes to CFs in the mice hearts. Further investigation revealed that the inflammasomes were activated in the cocultured CFs that connected with cardiomyocytes via membrane nanotubes (MNTs), a novel membrane structure that mediates distant intercellular connections and communication. Disruption of the MNTs with the microfilament polymerization inhibitor cytochalasin D (Cyto D) attenuated the inflammasome activation in the cocultured CFs. In addition, the MNT-mediated inflammasome activation in the CFs was blocked by deficiency of the inflammasome component NOD-like receptor protein 3 (NLRP3) in the cardiomyocytes, but not NLRP3 deficiency in the CFs. Moreover, ISO induced pyroptosis in the CFs cocultured with cardiomyocytes, and this process was inhibited by disruption of the MNTs with Cyto D or by the NLRP3 inhibitor MCC950 and the caspase-1 inhibitor Z-YVAD-FMK (FMK). Our study revealed that MNTs facilitate the rapid propagation of inflammasome activation among cardiac cells to promote pyroptosis in the early phase of β-adrenergic insult. Therefore, preventing inflammasome transfer is a potential therapeutic strategy to alleviate acute β-AR overactivation-induced cardiac injury.
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http://dx.doi.org/10.1038/s41419-020-03157-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648847PMC
November 2020

A peptide-based aggregation-induced emission bioprobe for selective detection and photodynamic killing of Gram-negative bacteria.

Biomater Sci 2021 Jan;9(2):437-442

Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin Stomatological Hospital, The Affiliated Stomatological Hospital of Nankai University, Tianjin 300041, China. and Key Laboratory of Bioactive Materials, Ministry of Education, and College of Life Sciences, Nankai University, Tianjin 300071, China.

Herein, we report a new bioprobe with aggregation-induced emission (AIE) characteristics by conjugation of a far-red/near-infrared emissive AIE luminogen and two polymyxinB peptides. Due to the strong binding effect between polymyxin B and the lipopolysaccharide in the cell wall of Gram-negative bacteria, the bioprobe can selectively visualize Gram-negative bacteria and effectively kill them via photodynamic treatment.
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http://dx.doi.org/10.1039/d0bm01330gDOI Listing
January 2021

Coupling Relationship Analysis between Quality Infrastructure and Ecological Environment Quality for Policy Implications.

Int J Environ Res Public Health 2020 10 19;17(20). Epub 2020 Oct 19.

College of Economics & Management, China Jiliang University, Hangzhou 310018, China.

Long-term improvement of ecological environment quality (EEQ) is a hotspot and urgent topic in the context of high-quality and sustainable development. It is urgent to look for methods that could support EEQ improvement in a high-quality and sustainable way. Owing to its natural supporting and guaranteeing functions for enhancing quality, quality infrastructure (QI) is a fundamental and critical element in promoting EEQ, but a neglected one. In this paper, we analyzed the coupling structure between QI and EEQ and applied an improved coupling model to recognize contributed and weakened indicators that affected the coupling relationship. We also examined this coupling relationship in the Yangtze River Delta (YRD) from 2012 to 2017, which proved the current situation where QI construction cannot satisfy the practical needs of EEQ improvement. Results showed that the important supporting role of QI in EEQ improvement should be valued for long-term sustainable development. Meanwhile, equilibrium and consistency of indicators in the QI and EEQ systems determined the coupling state. EEQ improvement countermeasures were also provided according to the coupling relationship analysis results. This study provided a scientific basis and guidance for EEQ improvement and sustainable development.
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http://dx.doi.org/10.3390/ijerph17207611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589060PMC
October 2020

Projecting the Influence of Global Warming on Physical Activity Patterns: a Systematic Review.

Curr Obes Rep 2020 Dec 17;9(4):550-561. Epub 2020 Oct 17.

Brown School, Washington University, St. Louis, MO, 63130, USA.

Purpose Of Review: This study systematically identified and synthesized empirical data-driven modeling studies that projected the future influence of global warming on people's physical activity patterns.

Recent Findings: A total of ten studies met the eligibility criteria and were included in the review. Global warming, in general, was predicted to be associated with a net increase in active commuting and leisure-time physical activity. However, the specific relationship between climate change and future physical activity patterns was expected to vary by the periods of prediction, geographical locations, population subgroups, and seasons. Moreover, the positive association between global warming and physical activity could level off or be reversed once reaching a temperature threshold. Preliminary evidence suggested a net increase in physical activity by mid- to late-twenty-first century owing to global warming. Future studies should refine the projections by taking into account the indirect effect of global warming through induced natural and human events, and assess effective countermeasures to mitigate the negative impact of global warming on vulnerable population subgroups.
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http://dx.doi.org/10.1007/s13679-020-00406-wDOI Listing
December 2020

Radiotherapy for Cervical Cancer in Patients with Systemic Lupus Erythematosus.

Cancer Manag Res 2020 21;12:8675-8683. Epub 2020 Sep 21.

Department of Radiation Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China.

Objective: To determine the toxicity and efficacy of radiotherapy for cervical cancer in patients with systemic lupus erythematosus (SLE).

Methods: Medical records of patients with SLE who received radiation for cervical cancer from January 2011 to January 2019 were reviewed. For definitive radiotherapy, a dose of 50.4 Gy in 28 fractions was delivered by intensity-modulated radiation therapy (IMRT) combined with high-dose-rate brachytherapy of 28 to 30 Gy in 5 fractions. A dose of 45 to 50.4 Gy in 25-28 fractions was delivered for postoperative radiation. All patients were in remission with or without a low dosage of prednisone or immunosuppressive medication. Survival data were analyzed with the Kaplan-Meier method. The incidence of severe toxicities among patients was compared with the chi-square test or Fisher's exact test.

Results: Twelve patients with SLE were included in this study. Definitive radiotherapy was delivered in 7 patients with FIGO (2009) stage II-III disease. Five patients with FIGO I disease received postoperative radiation. The median follow-up time was 22.1 months. The 3-year overall survival (OS) and 3-year progression-free survival (PFS) were 77.8% and 83.3%, respectively. One patient of definitive radiotherapy and one patient of postoperative radiation died due to distant metastasis of cervical cancer. Grade 3 or higher acute and chronic reactions occurred in 58.3% and 8.3% patients, respectively. Acute grade 3 or higher toxicity correlated with the presence of chemotherapy ( = 0.045).

Conclusion: Modern radiotherapy for cervical cancer was well tolerated in SLE patients with remission and provided a favorable outcome.
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http://dx.doi.org/10.2147/CMAR.S264795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518778PMC
September 2020

Clinical analysis of EBRT vs TLM in the treatment of early (T1-T2N0) glottic laryngeal cancer.

J Cancer 2020 23;11(22):6686-6694. Epub 2020 Sep 23.

Chinese Academy of Medical Sciences & Peking Union Medical College, NO.1 Shuaifuyuan Wangfujing, Dongcheng District, Beijing 100730, People's Republic of China.

To analyze the clinical efficacy of external beam radiation therapy (EBRT) vs transoral laser microsurgery (TLM) in patients with early glottic laryngeal carcinoma (T1-T2N0) and the effect of treatment choice on vocal function. A retrospective analysis of patients with T1-T2N0 glottic laryngeal carcinoma who underwent EBRT or TLM between January 2012 and December 2018 in PUMCH. The Kaplan-Meier method was used to analyze local control, progression-free survival and overall survival, and the VHI-30 scale was used to evaluate the effects of EBRT and TLM on vocal function. A total of 185 patients, all with pathologically confirmed squamous cell carcinoma, were enrolled. The median age was 62 years (38-88). N0 disease was confirmed by imaging: 142/185 (76.76%) patients had T1N0 disease, and 43/185 patients (23.24%) had T2/N0 disease. A total of 91/195 (49.19%) patients received an EBRT dose of 66-70 Gy/30-35f, at 2.0-2.3 Gy/f. 94/185 (50.81%) patients received TLM. The median follow-up time was 42 months (12-92), and the 3-year LC, PFS, and OS rates for the EBRT and TLM groups were 96.9% vs 94.1%(p=0.750), 95.3% vs 93.1%(p=0.993) and 93.3% vs 95.4%(p=0.467), respectively. The VHI-30 scales were used at the baseline showed no significant difference between the two groups 19.20±3.324 vs 21.65±9.80 (p=0.250), but the EBRT group had a low voice handicap after treatment, 10.24±6.093 vs 19.45±5.112 (p=0.001) (6 months) and 9.45±5.112 vs 14.97±7.741 (12 months). No CTCAE grade 3 or above side effects were observed in the EBRT group, but 3 cases of vocal cord stenosis were observed in the TLM group. The application of EBRT for early glottic laryngeal carcinoma (T1-T2N0) had an obvious curative effect with high LC and OS rates, no serious side effects, and a low voice handicap rate.
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http://dx.doi.org/10.7150/jca.46487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545664PMC
September 2020

Disruption of the Oxidative Pentose Phosphate Pathway Stimulates High-Yield Production Using Resting Corynebacterium glutamicum in the Absence of External Electron Acceptors.

Appl Environ Microbiol 2020 11 24;86(24). Epub 2020 Nov 24.

The National Food Institute, Technical University of Denmark, Kongens Lyngby, Denmark

Identifying and overcoming the limitations preventing efficient high-yield production of chemicals remain important tasks in metabolic engineering. In an attempt to rewire to produce ethanol, we attained a low yield (63% of the theoretical) when using resting cells on glucose, and large amounts of succinate and acetate were formed. To prevent the by-product formation, we knocked out the malate dehydrogenase and replaced the native E3 subunit of the pyruvate dehydrogenase complex (PDHc) with that from , which is active only under aerobic conditions. However, this tampering resulted in a 10-times-reduced glycolytic flux as well as a greatly increased NADH/NAD ratio. When we replaced glucose with fructose, we found that the glycolytic flux was greatly enhanced, which led us to speculate whether the source of reducing power could be the pentose phosphate pathway (PPP) that is bypassed when fructose is metabolized. Indeed, after shutting down the PPP by deleting the gene, encoding glucose-6-phosphate dehydrogenase, the ethanol yield on glucose increased significantly, to 92% of the theoretical. Based on that, we managed to rechannel the metabolism of into d-lactate with high yield, 98%, which is the highest that has been reported. It is further demonstrated that the PPP-inactivated platform strain can offer high-yield production of valuable chemicals using lactose contained in dairy waste as feedstock, which paves a promising way for potentially turning dairy waste into a valuable product. The widely used industrial workhorse possesses a complex anaerobic metabolism under nongrowing conditions, and we demonstrate here that the PPP in resting is a source of reducing power that can interfere with otherwise redox-balanced metabolic pathways and reduce yields of desired products. By harnessing this physiological insight, we employed the PPP-inactivated platform strains to produce ethanol, d-lactate, and alanine using the dairy waste whey permeate as the feedstock. The production yield was high, and our results show that inactivation of the PPP flux in resting cells is a promising strategy when the aim is to use nongrowing cells for producing valuable compounds. Overall, we describe the benefits of disrupting the oxidative PPP in nongrowing and provide a feasible approach toward waste valorization.
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http://dx.doi.org/10.1128/AEM.02114-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688235PMC
November 2020

Increased ABCC4 Expression Induced by ERRα Leads to Docetaxel Resistance via Efflux of Docetaxel in Prostate Cancer.

Front Oncol 2020 28;10:1474. Epub 2020 Aug 28.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, China.

Docetaxel is a major treatment for advanced prostate cancer (PCa); however, its resistance compromises clinical effectiveness. Estrogen receptor-related receptor alpha (ERRα) belongs to an orphan nuclear receptor superfamily and was recently found to be closely involved in cancer. In the present study, we found that ERRα was involved in docetaxel resistance in PCa. Overexpression of ERRα conferred docetaxel resistance in PCa cell lines, and cells with ERRα downregulation were more sensitive to docetaxel. Among the drug resistance-related genes, ABCC4 demonstrated synchronous expression after ERRα manipulation in cells. Moreover, both ERRα and ABCC4 were overexpressed in the docetaxel-resistant cell, which could be reversed by ERRα knockdown. The knockdown of ERRα also reversed the reduced drug accumulation in the docetaxel-resistant cell. We also demonstrated for the first time that ABCC4 was a direct target of ERRα as determined by the CHIP and luciferase assays. Bioinformatics analysis revealed high expression of ERRα and ABCC4 in PCa patients, and a number of potential ERRα/ABCC4 targets were predicted. In conclusion, our study demonstrated a critical role for ERRα in docetaxel resistance by directly targeting ABCC4 and stressed the importance of ERRα as a potential therapeutic target for drug-resistant PCa.
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http://dx.doi.org/10.3389/fonc.2020.01474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493678PMC
August 2020

Liquid chromatography-tandem mass spectrometric assay for TPN171 in human plasma.

J Pharm Biomed Anal 2020 Nov 17;191:113634. Epub 2020 Sep 17.

Shanghai Xuhui Central Hospital / Zhongshan- Xuhui Hospital, Fudan University, Shanghai 200031, China; Shanghai Engineering Research Center of Phase I Clinical Research & Quality Consistency Evaluation for Drugs, Shanghai 200031, China. Electronic address:

A simple, rapid and accurate method for quantitative analysis of a highly selective phosphodiesterase-5 inhibitor (PDE5), TPN171 by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using D-TPN171 as internal standards (ISTD). An aliquot of 100 μL of plasma was mixed with internal standard and was precipitated with acetonitrile. Gradient elution was performed on a ACQUITY HSS T3 column (50 × 2.1 mm, 1.8 μm) coupled with a ACQUITY column in-line filter at 40℃, by 5 mM ammonium acetate in water containing 0.1 % formic acid and 0.1 % formic acid in acetonitrile as the mobile phase. The total analytical run time was 3.5 min. The analyte was monitored using multiple reaction monitoring (MRM) scan in positive polarity mode. The ion transition was m/z 442.2→113.2 and 445.2→116.2 for TPN171 and D-TPN171 respectively. The method was validated for specificity, sensitivity, precision, accuracy, and other analytical parameters. The results found were satisfactory over the linear calibration range of 1-500 ng/mL. Within-day precisions ranged from 1.8 to 7.3 %, and between-day precisions from 2.3 to 4.9 %, accuracies were 95.5-99.8 %.The validated method was successfully applied to determine the plasma concentration after oral administration of 10 mg TPN171 in six healthy volunteers.
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http://dx.doi.org/10.1016/j.jpba.2020.113634DOI Listing
November 2020

Conditional reprogramming: next generation cell culture.

Acta Pharm Sin B 2020 Aug 26;10(8):1360-1381. Epub 2020 Jan 26.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou 646000, China.

Long-term primary culture of mammalian cells has been always difficult due to unavoidable senescence. Conventional methods for generating immortalized cell lines usually require manipulation of genome which leads to change of important biological and genetic characteristics. Recently, conditional reprogramming (CR) emerges as a novel next generation tool for long-term culture of primary epithelium cells derived from almost all origins without alteration of genetic background of primary cells. CR co-cultures primary cells with inactivated mouse 3T3-J2 fibroblasts in the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling primary cells to acquire stem-like characteristics while retain their ability to fully differentiate. With only a few years' development, CR shows broad prospects in applications in varied areas including disease modeling, regenerative medicine, drug evaluation, drug discovery as well as precision medicine. This review is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its wide applications, highlighting the value of CR in both basic and translational researches and discussing the challenges faced with CR.
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http://dx.doi.org/10.1016/j.apsb.2020.01.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488362PMC
August 2020

Intensity-modulated Radiotherapy for Pituitary Somatotroph Adenomas.

J Clin Endocrinol Metab 2020 12;105(12)

Department of Radiation Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Objective: To summarize our experience in the treatment of pituitary somatotroph adenomas by fractionated intensity-modulated radiotherapy (IMRT), describe the treatment outcomes, and determine predictors.

Methods And Materials: Patients with pituitary somatotroph adenoma treated by IMRT in our institution from August 2009 to January 2019 were reviewed. A total of 113 patients (37 male) were included in this study. The median age was 33 years (range 12-67 years). A total of 112 patients had not achieved complete remission after surgery, and 1 patient was treated by radiotherapy (RT) alone because she refused to surgery. The median growth hormone level was 8.6 ng/mL (range 2-186 ng/mL) and the median insulin-like growth factor (IGF)-1 level was 732 ng/mL (range 314-1485 ng/mL) pre-RT. The radiation doses to clinical target volume were usually 50-56 Gy in 25 to 30 fractions and to gross tumor volume were 60.2 Gy in 28 fractions while simultaneous integrated boost-IMRT used. After RT, the patients were followed up with endocrine testing every 6 to 12 months and magnetic resonance imaging annually. Endocrine complete remission was defined as a normal sex- and age-adjusted IGF-1 level without any pituitary suppressive medications. The outcomes including endocrine remission and new hypopituitarism after RT were recorded. The median follow-up time was 36 months (range 6-105.5 months).

Results: The endocrine complete remission rates of IGF-1 at 1, 2, 3, and 5 years were 6%, 22.8%, 48.6%, and 74.3%, respectively. The median time to complete remission was 36.2 ± 3.8 months. The tumor control rate was 99% during the follow-up. The overall incidence of RT-induced hypopituitarism was 28.3% at the last follow-up. Univariate and multivariate analysis demonstrated that tumor sizes before RT, pre-RT IGF-1 level, and age significant predicted the endocrine remission.

Conclusions: IMRT is a highly effective treatment for pituitary somatotroph adenoma. Endocrine remission rate, tumor control rate, the median time to remission and hypopituitarism incidence are similar to stereotactic radiosurgery. Age and IGF-1 level before RT were significant predictive factors in endocrine remission.
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http://dx.doi.org/10.1210/clinem/dgaa651DOI Listing
December 2020