Publications by authors named "Jing Ma"

1,885 Publications

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Corrigendum: PKM2-Induced the Phosphorylation of Histone H3 Contributes to EGF-Mediated PD-L1 Transcription in HCC.

Front Pharmacol 2021 15;12:724799. Epub 2021 Jul 15.

Institute for Innovative Drug Design and Evaluation, School of Pharmacy, Henan University, Kaifeng, China.

[This corrects the article DOI: 10.3389/fphar.2020.577108.].
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http://dx.doi.org/10.3389/fphar.2021.724799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320321PMC
July 2021

[Corrigendum] Linc01105 acts as an oncogene in the development of neuroblastoma.

Oncol Rep 2021 Sep 28;46(3). Epub 2021 Jul 28.

Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai 201102, P.R. China.

Subsequently to the publication of the above article, the authors have found that Fig. 4A on p. 1532 contained some errors. Owing to mistakes made during the preparation and revision of the manuscript, the invasion assay data images selected to show both the 'Control' and 'shRNA2' groups of the invasion and migration experiments were derived from the same original sources. A corrected version of the Fig. 4, showing the correct data for the invasion and migration assay experiments with the Control and shRNA2 groups, is shown below. These inadvertent errors did not affect the conclusions reported in this paper, and all the authors agree with this Corrigendum. The authors thank the editor of for presenting them with the opportunity to publish this Corrigendum, and apologize to the editor and to the readership of the journal for any inconvenience caused. [the original article was published in Oncology Reports 42: 1527-1538, 2019; DOI: 10.3892/or.2019.7257].
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http://dx.doi.org/10.3892/or.2021.8156DOI Listing
September 2021

Effects of rotation grazing time on the characteristics of soil seed bank in desert steppe.

Ying Yong Sheng Tai Xue Bao 2021 Jul;32(7):2378-2388

Institute of Agriculture and Animal Husbandry Science, Science and Technology Bureau of Yanchi County, Ningxia 751500, China.

Exploring the characteristics of soil seed bank under seasonal grazing plays an important role in the reasonable use of desert steppe. We examined seed bank species composition, density, vertical distribution, diversity in the soil of 0-5, 5-10, 10-15 cm soil layers and the relationship with vegetation and soil environmental factors in Ningxia desert steppe. The treatments included enclosure (FY), traditional time rotation grazing (FG), delayed to start rotation grazing (YG), early to end rotation grazing (TG), delayed to start and early to end rotation grazing (YT) and free grazing (ZY). The results showed that there were nine families and 21 species in the soil seed bank in the study area. The species composition of soil seed bank in TG seed bank with 13 species was the largest, while FY and ZY soil seed banks had the least, with 8 species, respectively. The den-sity of ZY soil seed bank was significantly higher than that of FY, YG, TG and YT. The number of perennial species seeds in the soil seed bank of FY was the largest, reaching 32.0%, while that in the ZY was the least, only 12.4%, mainly with sexually reproduced annual weeds. Soil seed bank was mainly concentrated in the 0-5 cm surface layer. With increasing soil depth, the size of soil seed bank decreased. The dominance and diversity of soil seed bank were the highest in FY, while the dominance, diversity and uniformity were the lowest in ZY. The similarity between soil seed bank and aboveground vegetation was generally low. In contrast, the FG and ZY seed banks had higher similarity with vegetation, and the FY was the lowest. Soil moisture, organic matter, total nitrogen concentration, total phosphorus concentration, and soil bulk density were the main soil factors affecting soil seed bank. Compared with traditional whole year free grazing, seasonal four-zone rotation grazing could increase species richness and abundance of perennial plants in soil seed bank of desert steppe, as well as the total species richness, diversity and uniformity of the seed bank. Although the effect sof seasonal four-zone rotation grazing on seed bank was not as good as the long-term enclosure grassland, it was of great significance to the utilization and protection of desert steppe.
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http://dx.doi.org/10.13287/j.1001-9332.202107.022DOI Listing
July 2021

[Effect of Elevated CO on NO Emissions from Different Rice Cultivars in Rice Fields].

Huan Jing Ke Xue 2021 Aug;42(8):3924-3930

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.

Using the free air CO enrichment (FACE) platform, an in-situ field experiment was conducted to explore the impacts of elevated CO mole fraction ([CO]) on NO emissions from strongly and weakly responsive rice cultivars. Under elevated [CO], grain yield of the strongly responsive rice cultivars increased significantly, by more than 30%, whereas the weakly responsive cultivars showed a growth rate of 10%-15%. The four treatments comprised A-W (normal [CO]+weakly responsive cultivar), F-W (elevated [CO]+weakly responsive cultivar), A-S (normal [CO]+strongly responsive cultivar), and F-S (elevated [CO]+strongly responsive cultivar). Compared to the normal [CO] treatments (A-S and A-W), when the strongly and weakly responsive cultivars were exposed to elevated [CO](F-S and F-W), NO emissions decreased by 52.54% (<0.05) and 38.40% (<0.05), rice yield increased by 22.96% (<0.05) and 12.11% (>0.05), and NO emission intensity decreased by 61.68% (<0.05) and 45.13% (<0.05), respectively. Moreover, NO emissions of all treatments were significantly positively correlated with NH-N content (<0.05), whereas not correlated with NO-N content. Soil temperature is an important factor affecting the NO emissions of the strongly responsive cultivar in rice fields under elevated [CO] conditions. Through comprehensive consideration of climate conditions, in the future, priority should be given to planting the strongly responsive cultivar, ensuring high rice yield and significant reduction in NO emissions.
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http://dx.doi.org/10.13227/j.hjkx.202012211DOI Listing
August 2021

Regulation of the long noncoding RNA XIST on the inflammatory polarization of microglia in cerebral infarction.

Exp Ther Med 2021 Sep 30;22(3):924. Epub 2021 Jun 30.

Meteorological Service and Applied Meteorology, Ren County Meteorological Bureau of Hebei Province, Xingtai, Hebei 055150, P.R. China.

Proinflammatory polarization of microglia aggravates brain injury in cerebral infarction. The present study focused on the role of long non-coding (lnc)RNA X-inactive specific transcript (XIST) in the phenotype modulation of microglia. It was revealed that lncRNA XIST was significantly upregulated in both a mouse cerebral infarction model induced by middle cerebral artery occlusion (MCAO) and an activated microglial model induced by oxygen/glucose deprivation (OGD). The overexpression of XIST enhanced the expression and release of pro-inflammatory mediators [such as tumor necrosis factor (TNF)-α, IL-6, and iNOS] in microglia. Culture supernatant from lncRNA XIST-overexpressed microglial cells induced the apoptosis of primary neurons, while TNF-α antibody counteracted this neurotoxic effect. LncRNA XIST served as a sponge for miR-96-5p, counteracting its inhibitory effect on IKKβ/NF-κB signaling and TNF-α production. Notably, TNF-α was positively regulated by XIST and in turn enhanced XIST expression in microglia. The lncRNA XIST-TNF-α feedback promoted the proinflammatory polarization of microglia, thereby exacerbating cerebral neuron apoptosis.
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http://dx.doi.org/10.3892/etm.2021.10356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281447PMC
September 2021

[Genotype and phenotype analysis of a family with Waardenburg syndrome type Ⅰcaused by a novel mutation in gene].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Jul;35(7):621-626

Department of Otolaryngology Head and Neck Surgery,Kunming Children's Hospital,(Children's Hospital Affiliated to Kunming Medical University),Kunming,650228,China.

To identify gene mutation and analysis the association between clinical characterizes and the mutations in a family of Waardenburg syndrome (WS) type I in Yunnan, China. With informed consent, the proband with WS phenotype and his family members were given medical history collection, physical examination and audiological evaluation. Peripheral blood was obtained, genomic DNA was extracted, and deafness related genes were detected by high-throughput sequencing. Sanger sequencing was used to verify the mutation sites of proband and his family members. C. 602C>G mutation in exon 5 of gene was identified, which is nonsense mutation and may cause a truncated protein. The mutation cause 201 amino acid of the protein changed from serine to stop codon. According to the American College of Medical Genetics and Genomics (ACMG), it is considered as Pathogenicity(PVS1+PM2+PP3). This mutation has not been included in the database also not been reported in the literature. Combined with the results of clinical diagnosis and gene diagnosis, this mutation was considered as the cause of the disease. This study enriched mutation spectrum of gene.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.07.010DOI Listing
July 2021

Development & validation of LC-MS/MS assay for 5-amino-1-methyl quinolinium in rat plasma: Application to pharmacokinetic and oral bioavailability studies.

J Pharm Biomed Anal 2021 Jul 8;204:114255. Epub 2021 Jul 8.

Department of Pharmaceutical Sciences, Texas Southern University, Houston, TX, 77004, United States. Electronic address:

5-Amino-1-methyl quinolinium (5-AMQ) is a potent Nicotinamide N-methyl transferase (NNMT) inhibitor. NNMT is an enzyme that catalyzes the N-methylation of the endogenous substrate nicotinamide, as well as exogenous xenobiotics. NNMT is fundamental to cellular metabolism; NNMT is overexpressed in select tissues (e.g., adipose tissue, skeletal muscle, etc.) in pathophysiological conditions, making it a clinically relevant target for drug development in several chronic diseases including obesity and diabetes. The objective of this study was to develop and validate a simple, sensitive, and reproducible liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5-AMQ in rat plasma and urine samples. 5-AMQ was extracted from plasma and urine by protein precipitation. Chromatographic separation was achieved using an ACE® Excel™ C column (2 μm, 50 × 2.1 mm) with a binary gradient solvent system comprising of water (A) and acetonitrile (B) containing 0.1 % formic acid as the mobile phase. Analysis was performed using an API 4000 QTRAP hybrid triple quadruple mass spectrometer and multiple reaction monitoring (MRM) in positive mode at m/z transitions of 159.100 → 90.00 and 162.200 → 117.200 for 5-AMQ and the internal standard, respectively. The standard curves of 5-AMQ in rat urine and plasma samples were linear in the concentration range of 10-2500 ng/mL. The intra-day and inter-day precisions and accuracies for 5-AMQ at four concentration levels in rat plasma and urine samples were found to be within the 15 % FDA acceptance range. Similarly, the accuracy and precision of 5-AMQ quantification in samples diluted up to 20-fold using blank plasma were within the 15 % acceptable range. Furthermore, the extraction recoveries and matrix effects at three concentration levels of rat plasma samples ranged from 99.5 %-110.6 % and -6.1 %-14.1 %, respectively. 5-AMQ was stable in rat plasma samples subjected to standard storage, preparation, and handling conditions, with less than 15 % variation noted at two concentration levels. The validated, sensitive, and reproducible LC-MS/MS method for 5-AMQ in rat plasma and urine samples was effectively applied to a pharmacokinetic study in rats with IV and oral administration of 5-AMQ. 5-AMQ displayed substantial plasma exposures via IV and oral route, with a mean maximum plasma concentration of 2252 ng/mL after oral administration, mean area under the curve (AUC) of 3708 h.ng/mL and 14431 h.ng/mL for the IV and oral groups, respectively, mean terminal elimination half-life of 3.80 ± 1.10 h and 6.90 ± 1.20 h respectively after intravenous and oral dose, and a good oral bioavailability (F % = 38.4).
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http://dx.doi.org/10.1016/j.jpba.2021.114255DOI Listing
July 2021

Dynamic expression of vascular endothelial growth factor (VEGF) and platelet-derived growth factor receptor beta (PDGFRβ) in diabetic brain contributes to cognitive dysfunction.

Brain Res Bull 2021 Jul 23;175:99-106. Epub 2021 Jul 23.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China. Electronic address:

Background: Cognitive dysfunction is increasingly recognized as an important complication of diabetes mellitus (DM). Accumulating evidence indicates that the abnormality of cerebrovascular structure and function plays an essential role in diabetic cognitive impairment (DCI), however, changes in cerebrovascular factors have been blurred during the development of diabetes.

Objective: To evaluate the changes in the structure and function of cerebrovascular in DCI mice and to investigate the changes of cerebral angiogenesis and stability factors during the development of DM.

Methods: Diabetes was induced by feeding with high-fat diet combined with intraperitoneal injection of streptozotocin (STZ,120 mg/kg). Cognitive function was evaluated at different stages of DM, cerebral neovascularization, blood-brain barrier (BBB) permeability and hippocampal neurons were measured of DCI mice, and the expression of vascular endothelial growth factor (VEGF) and platelet-derived growth factor receptor β (PDGFRβ) in hippocampus was detected during the development of DM.

Results: With the progress of diabetes, the learning and memory ability of mice gradually decreased, and DCI mice showed neuronal degeneration, increased BBB permeability and pathological cerebral neovascularization. Moreover, the expression of VEGF in the hippocampus increased first and then decreased at DM+8week, PDGFRβ decreased continuously with the development of diabetes.

Conclusions: Our results demonstrate that DCI may be attributed to the dynamic expression of VEGF/PDGFRβ in diabetic hippocampus, and pathological cerebral neovascularization, increased BBB permeability and neuronal degeneration are the key links.
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http://dx.doi.org/10.1016/j.brainresbull.2021.07.017DOI Listing
July 2021

Erythropoietin promotes abdominal aortic aneurysms in mice through angiogenesis and inflammatory infiltration.

Sci Transl Med 2021 07;13(603)

Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China.

Abdominal aortic aneurysm (AAA) is a potentially fatal vascular disease, but the underlying mechanisms remain unknown. Here, we tested the hypothesis that erythropoietin (EPO) may promote the formation of AAA. We found that EPO dose-dependently promoted the formation of AAA in both (66.7%) and wild-type (WT) (60%) mice receiving a high dose of EPO. EPO monoclonal antibodies given to mice receiving angiotensin II (AngII) stimulation resulted in a markedly lower incidence of AAA (from 86.7 to 20%, < 0.001), and EPO receptor (EPOR) knockdown in mice substantially reduced the incidence of AAA compared to mice after AngII stimulation (from 86.7 to 45.5%, < 0.05), further supporting the finding that EPO is a contributor to AAA formation. EPO-induced AAA resulted in increased microvessels, phagocyte infiltration, and matrix metalloproteinase secretion, as well as reduced collagen and smooth muscle cells (SMCs). Experiments in vitro and ex vivo demonstrated that EPO induced proliferation, migration, and tube formation of endothelial cells via the JAK2/STAT5 signaling pathway. In humans, serum EPO concentrations were higher in patients with AAA than in healthy individuals and correlated with the size of the AAA, suggesting a potential link between EPO and the severity of AAA in humans. In conclusion, we found that EPO promotes the formation of AAA in both and WT mice by enhancing angiogenesis, inflammation, collagen degradation, and apoptosis of SMCs and that EPO/EPOR signaling is essential for AngII-induced AAA. The association between EPO and AAA in humans warrants further study.
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http://dx.doi.org/10.1126/scitranslmed.aaz4959DOI Listing
July 2021

lncRNA PROX1-AS1 mediates the migration and invasion of placental trophoblast cells via the miR-211-5p/caspase-9 axis.

Bioengineered 2021 Dec;12(1):4100-4110

Obstetrics Department, First Affiliated Hospital of Kunming Medical University, Kunming, China.

Preeclampsia (PE) is a potentially fatal pregnancy complication; however, its pathogenesis remains unclear. Long non-coding RNAs (lncRNAs) are associated with occurrence and progression of PE. Therefore, this study aimed to explore the function of the lncRNA prospero homeobox 1-antisense RNA 1 (PROX1-AS1) and elucidate its underlying molecular mechanism of action. We found that the expression levels of PROX1-AS1 were elevated in both the placental tissues and blood samples of the patients with PE. Moreover, the results of the flow cytometry and transwell assay showed that the knockdown of PROX1-AS1 inhibited the apoptosis and promoted the migration and invasion of HTR-8/SVneo cells. We also assessed the interactions between PROX1-AS1, caspase-9, and microRNA (miR)-211-5p via dual-luciferase reporter and RNA pull-down analyses. The data indicated that PROX1-AS1 acted as a sponge for miR-211-5p to regulate the expression of caspase-9. Moreover, the expression of miR-211-5p was reduced in PE and negatively related to PROX1-AS1, while that of caspase-9 was increased in PE and negatively regulated by miR-211-5p. Furthermore, inhibition of miR-211-5p rescued the facilitation of cell apoptosis, migration and invasion induced by the knockdown of PROX1-AS1. We also found that caspase-9 improved the apoptosis rate, and suppressed the cell migration and invasion induced by the overexpression of miR-211-5p. In conclusion, the knockdown of PROX1-AS1 promoted the cell morbidity of the trophoblast cells by modulating the miR-211-5p/caspase-9 axis, which may alleviate the progression of PE. This novel regulatory network may contribute to the pathogenesis and progression of PE.
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http://dx.doi.org/10.1080/21655979.2021.1953213DOI Listing
December 2021

Synergistic Interface Layer Optimization and Surface Passivation with Fluorocarbon Molecules toward Efficient and Stable Inverted Planar Perovskite Solar Cells.

Research (Wash D C) 2021 28;2021:9836752. Epub 2021 Jun 28.

State Key Discipline Laboratory of Wide Band Gap Semiconductor Technology, Shaanxi Joint Key Laboratory of Graphene, School of Microelectronics, Xidian University, 2 South Taibai Road, Xi'an 710071, China.

Large-size organic halide passivation has been considered an efficient approach to enhance the perovskite solar cell (PSC) efficiency and stability. Herein, a facile posttreatment strategy was demonstrated, wherein trifluoromethyl-phenethylamine hydrobromide (CF-PEABr) is firstly used to passivate the perovskite film surface. The CF-PEABr surface posttreatment could coordinate with halide dangling bonds that exist at the perovskite crystal surface. Moreover, the surface treatment with CF-PEABr could efficiently passivate the defects in the perovskite film and suppress the nonradiative carrier recombination. As a result, a high efficiency of 21.3% is obtained, and an increment of 80 mV in (a large of 1.15 V, with a 0.42 V voltage deficit) occurs, compared to the control device. To relieve the hydrophobic nature properties of the -CF functional group and the dewetting problem of PCBM layer deposition, a surfactant Triton X-100 is used to modify the PCBM layer. Furthermore, the devices with CF-PEABr posttreatment exhibit better operational, thermal (85°C), and long storage stabilities without any encapsulation.
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http://dx.doi.org/10.34133/2021/9836752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261667PMC
June 2021

Molecular Tailoring of n/p-type Phenothiazine Organic Scaffold for Zinc Batteries.

Angew Chem Int Ed Engl 2021 Jul 18. Epub 2021 Jul 18.

Donghua University, College of Chemistry, CHINA.

P-type or n-type redox reactions of organics are being used as the reversible electrodes to build the next-generation rechargeable batteries with sustainable and tunable characteristics. However, the n-type organics that store cations generally exhibit low potential (< 0.8 V vs. Zn/Zn 2+ ), while the p-type organics that store anions suffer from limited capacity (< 100 mAh g -1 ). Here we demonstrate that bis(phenylamino)phenothiazin-5-ium iodide (PTD-1) containing both n-type and p-type redox moieties exhibits a hybrid charge storage mechanism (i.e., n/p-type at low potential + p-type at high potential) by using experiments and theoretical calculations. It is found that such hybrid mechanism combines the advantages of n and p-type reactions and compensates for the drawback of each other. Accordingly, the aqueous Zn//PTD-1 full cell shows a high voltage (e.g., 1.8 V maximum or 1.1 V average ), a high capacity 188.24 mAh g PTD-1 -1 (achieved at 40 mA g -1 ), a long-life and a supercapacitor-like high power. These results shed new light on the design of advanced organic electrodes.
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http://dx.doi.org/10.1002/anie.202106238DOI Listing
July 2021

Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis .

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Urology Surgery, Kunming Children's Hospital, Kunming, Yunnan 650228, P.R. China.

Wilms' tumor (WT) is a major type of kidney cancer in children; however, the therapeutic measures for control of tumor metastasis, recurrence and death for this type of cancer remain unsatisfactory. The present study aimed to verify the expression of T‑cell factor 3 (TCF3) in WT, and to explore its role in regulating the viability, migration and apoptosis of kidney tumor cells. Tumor tissues were collected from 10 patients with WT, and adjacent tissues were collected as normal controls. The expression levels of TCF3 were detected in WT tissues and adjacent tissues by reverse transcription‑quantitative PCR (RT‑qPCR), western blotting and immunohistochemistry. In addition, TCF3 expression was silenced in G401 kidney tumor cells via small interfering RNA transfection. Cell viability, cell cycle progression and cell apoptosis were assessed using the MTT assay and flow cytometry; the migration and invasion of kidney tumor cells were examined using Transwell and wound‑healing assays; and the expression levels of Wnt signaling pathway‑related genes (Wnt1, β‑catenin and c‑myc) were detected by RT‑qPCR and western blotting. The results revealed that the expression levels of TCF3 were high in WT tissues from patients. Silencing TCF3 expression in G401 kidney tumor cells significantly inhibited cell viability and migration, and promoted cell apoptosis. Moreover, silencing TCF3 expression in G401 cells inhibited the expression levels of Wnt signaling pathway‑related genes. Overall, these data indicated that TCF3 may be involved in WT development through regulation of Wnt signaling pathways. The findings of the present study provide a novel potential marker for the treatment and prognostic evaluation of WT.
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http://dx.doi.org/10.3892/mmr.2021.12281DOI Listing
September 2021

SP1-Mediated Upregulation of Long Noncoding RNA ZFAS1 Involved in Non-syndromic Cleft Lip and Palate Inactivating WNT/β-Catenin Signaling Pathway.

Front Cell Dev Biol 2021 29;9:662780. Epub 2021 Jun 29.

ENT Institute, Department of Facial Plastic and Reconstructive Surgery, Eye & ENT Hospital, Fudan University, Shanghai, China.

Non-syndromic cleft lip and palate (NSCLP) is one of the most common congenital malformations with multifactorial etiology. Although long non-coding RNAs (lncRNAs) have been implicated in the development of lip and palate, their roles in NSCLP are not fully elucidated. This study aimed to investigate how dysregulated lncRNAs contribute to NSCLP. Using lncRNA sequencing, bioinformatics analysis, and clinical tissue sample detection, we identified that lncRNA ZFAS1 was significantly upregulated in NSCLP. The upregulation of ZFAS1 mediated by SP1 transcription factor (SP1) inhibited expression levels of Wnt family member 4 () through the binding with CCCTC-binding factor (CTCF), subsequently inactivating the WNT/β-catenin signaling pathway, which has been reported to play a significant role on the development of lip and palate. Moreover, , the overexpression of ZFAS1 inhibited cell proliferation and migration in human oral keratinocytes and human umbilical cord mesenchymal stem cells (HUC-MSCs) and also repressed chondrogenic differentiation of HUC-MSCs. , ZFAS1 suppressed cell proliferation and numbers of chondrocyte in the zebrafish ethmoid plate. In summary, these results indicated that ZFAS1 may be involved in NSCLP by affecting cell proliferation, migration, and chondrogenic differentiation through inactivating the WNT/β-catenin signaling pathway.
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http://dx.doi.org/10.3389/fcell.2021.662780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275830PMC
June 2021

Differentially expressed microRNA in testicular tissues of hyperuricaemia rats.

Andrologia 2021 Jul 13:e14184. Epub 2021 Jul 13.

Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China.

This study is to identify the differentially expressed miRNAs in testicular tissues of rats with hyperuricaemia-induced male infertility. We found that the hyperuricaemia model group had significantly increased serum uric acid, while significantly decreased sperm concentration and motile sperm percentage than normal group (p < .05). A total of 39 differentially expressed miRNAs were identified in the testicular tissues of hyperuricaemia rats compared with the control rats, ten of which were validated by real-time PCR. The target mRNAs of 7 differentially expressed miRNAs (miR-10b-5p, miR-26a-5p, miR-136-5p, miR-151-3p, miR-183-5p, miR-362-3p and miR-509-5p) from 3'-untranslated region binding perspective were enriched in signalling pathways of Wnt, Jak-STAT, mTOR and MAPK. The target mRNAs of 6 differentially expressed miRNAs (miR-136-5p, miR-144-3p, miR-99a-5p, miR-509-5p, miR-451-5p and miR-362-3p) from coding sequence binding perspective were enriched in signalling pathways of Calcium, Notch and MAPK. The functions of miRNAs in testicular tissues of rats with hyperuricaemia were revealed by the differentially expressed miRNAs (miR-183-5p, miR-99a-5p, miR-10b-5p, miR-151-3p, miR-26a-5p, miR-451-5p, miR-362-3p, miR-136-5p, miR-144-3p and miR-509-5p)-mRNAs interaction network. The differentially expressed miRNAs in the testicular tissues of hyperuricaemia rats might shed light on the mechanism of hyperuricaemia-induced male infertility.
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http://dx.doi.org/10.1111/and.14184DOI Listing
July 2021

Multifunctional antibacterial films with silver nanoparticles reduced in situ by lemon juice.

Food Chem 2021 Jul 1;365:130517. Epub 2021 Jul 1.

School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou 363000, China.

Microorganisms contribute to deterioration of garden stuff after picking. Antibacterial films consisting of polyvinyl alcohol (PVA), soluble starch (SS), and silver nanoparticles (AgNPs) with AgNPs in situ reduced from AgNO by lemon juice (LJ) were developed to combat microbial corruption. PVA/SS/LJ/AgNPs films have better tensile strength and elongation at break, but lower water vapor permeability, moisture absorption, and transmittance compared to PVA/SS and PVA/SS/LJ films. Silver released from PVA/SS/LJ/AgNPs films were 13.55, 18.97, 19.55, 20.19, 20.47, and 20.82 mg/g after submerging samples in water for three seconds and soaking for 1, 2, 3, 4, and 5 days, respectively. PVA/SS/LJ/AgNPs films had good bacteriostatic effects on Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Salmonella, as the average diameter of the inhibition circle was 12.12 ± 0.01, 13.04 ± 0.02, 14.79 ± 0.01, 14.20 ± 0.01, and 12.68 ± 0.01 mm, respectively. Therefore, PVA/SS/LJ/AgNPs film has a wide application prospect.
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http://dx.doi.org/10.1016/j.foodchem.2021.130517DOI Listing
July 2021

Recent advances in the epigenetics of bone metabolism.

J Bone Miner Metab 2021 Jul 11. Epub 2021 Jul 11.

Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.

Osteoporosis is a common form of metabolic bone disease that is costly to treat and is primarily diagnosed on the basis of bone mineral density. As the influences of genetic lesions and environmental factors are increasingly studied in the pathological development of osteoporosis, regulated epigenetics are emerging as the important pathogenesis mechanisms in osteoporosis. Recently, osteoporosis genome-wide association studies and multi-omics technologies have revealed that susceptibility loci and the misregulation of epigenetic modifiers are key factors in osteoporosis. Over the past decade, extensive studies have demonstrated epigenetic mechanisms, such as DNA methylation, histone/chromatin modifications, and non-coding RNAs, as potential contributing factors in osteoporosis that affect disease initiation and progression. Herein, we review recent advances in epigenetics in osteoporosis, with a focus on exploring the underlying mechanisms and potential diagnostic/prognostic biomarker applications for osteoporosis.
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http://dx.doi.org/10.1007/s00774-021-01249-8DOI Listing
July 2021

Dyslipidemia Is Related to Mortality in Critical Patients With Coronavirus Disease 2019: A Retrospective Study.

Front Endocrinol (Lausanne) 2021 23;12:611526. Epub 2021 Jun 23.

Department of Endocrinology and Metabolism, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: It has been reported that dyslipidemia is related to coronavirus-related diseases. Critical patients with coronavirus disease 2019 (COVID-19) who suffered from multiple organ dysfunctions were treated in the intensive care unit (ICU) in Wuhan, China. Whether the lipids profile was associated with the prognosis of COVID-19 in critical patients remained unclear.

Methods: A retrospective study was performed in critical patients (N=48) with coronavirus disease 2019 in Leishenshan hospital between February and April 2020 in Wuhan. The parameters including lipid profiles, liver function, and renal function were collected on admission day, 2-3days after the admission, and the day before the achievement of clinical outcome.

Results: Albumin value and creatine kinase (ck) value were statistically decreased at 2-3 days after admission compared with those on admission day (<0.05). Low density lipoprotein (LDL-c), high density lipoprotein (HDL-c), apolipoprotein A (ApoA), and apolipoprotein A (Apo B) levels were statistically decreased after admission (<0.05). Logistic regression showed that HDL-c level both on admission day and the day before the achievement of clinical outcome were negatively associated with mortality in critical patients with COVID-19. Total cholesterol (TC) level at 2-3days after admission was related to mortality in critical patients with COVID-19.

Conclusions: There were lipid metabolic disorders in the critical patients with COVID-19. Lower levels of HDL-c and TC were related to the progression of critical COVID-19.
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http://dx.doi.org/10.3389/fendo.2021.611526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261125PMC
July 2021

Allergic Asthma-Induced Cognitive Impairment is Alleviated by Dexamethasone.

Front Pharmacol 2021 23;12:680815. Epub 2021 Jun 23.

Institute of Neuroscience, Basic Medical College, Chongqing Medical University, Chongqing, China.

Allergic asthma is a typical chronic inflammatory disease of respiratory tract. Clinical data shows that patients with allergic asthma have different degrees of cognitive dysfunction. The molecular mechanism underlying the pathogenesis of asthma-induced cognitive disorder is not yet well defined. Dexamethasone (DEX), one of the first-line drugs being widely used in the treatment of asthma, has not been reported to have an effect on cognitive dysfunction in mice model. To investigate the effect of asthma on cognitive impairment as well as the effect of DEX on asthma-caused morphological and behavioral changes, C57BL/6J mice received treatment with house dust mites (HDM) for 60 days to become allergic asthma model mice, and a group of HDM-treated asthma model mice were treated with DEX. HDM-treated asthma model mice exhibited increased airway hyperresponsiveness (AHR) and inflammatory infiltration in lung tissue. An elevated level of IL-4, IL-5, and TNF-α was detected in bronchoalveolar lavage fluid (BALF) by Luminex liquid suspension chip. Asthma model mice also presented memory deficits accompanied with morphological changes at the synaptic levels in the cortex and hippocampus. Meanwhile, vascular edema and increased expression of HIF-1α and HIF-2α were found in the brain of asthma model mice. Interestingly, DEX treatment could reverse the inflammatory changes in asthma model mice airway, rescue the cognitive impairment and improve the synaptic plasticity. Besides, DEX significantly decreased the expression of HIF-1α and HIF-2α in mice brain and lung. These processes may be used to decipher the complex interplay and pathological changes between asthma and cognition. This study provides laboratory evidence for the prevention and treatment of cognitive malfunction induced by asthma.
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http://dx.doi.org/10.3389/fphar.2021.680815DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261293PMC
June 2021

Epidemiology of Alcohol-Associated Liver Disease.

Clin Liver Dis 2021 08 26;25(3):483-492. Epub 2021 May 26.

Division of Gastroenterology and Hepatology, Department of Medicine, Indiana University School of Medicine, 702 Rotary Circle, Indianapolis, IN 46202, USA; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA; Roudebush Veterans Administration Medical Center, Indianapolis, IN, USA. Electronic address:

Alcohol-associated liver disease (ALD) is a consequence of excessive alcohol use. It comprises a spectrum of histopathologic changes ranging from simple steatosis, steatohepatitis, and cirrhosis to hepatocellular carcinoma. The public health impact of ALD is growing because of an increase in the prevalence and incidence of ALD in parallel with liver transplant and mortalities. There are multiple factors involved in the pathogenesis and progression of ALD. Reducing alcohol consumption is the cornerstone of ALD management. The efforts to reduce excessive alcohol use at the individual and population levels are urgently needed to prevent adverse outcomes from ALD.
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http://dx.doi.org/10.1016/j.cld.2021.03.009DOI Listing
August 2021

Tribological, cytotoxicity and antibacterial properties of graphene oxide/carbon fibers/polyetheretherketone composite coatings on Ti-6Al-4V alloy as orthopedic/dental implants.

J Mech Behav Biomed Mater 2021 Jun 30;122:104659. Epub 2021 Jun 30.

School of Material Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, China. Electronic address:

In this work, graphene oxide/carbon fibers/polyetheretherketone (GO/CF/PEEK) composite coatings on Ti-6Al-4V (TC4) alloy were fabricated by electrostatic powder spraying method. The coatings with 0.02 wt% GO and 25 wt% CF were made to improve the wear resistance, cytocompatibility and antibacterial properties of the TC4 as orthopedic/dental implants. The physicochemical properties involving coating thickness, Vickers hardness, micromorphology, phase structures and contact angles were investigated. The results indicated that the GO/CF/PEEK coatings can significantly decrease the coefficient of friction (COF) (from 0.433 ± 0.017 to 0.085 ± 0.008) and enhance the wear resistance of TC4 alloy during the wet friction process in sliding contact with a SiN ball. The results showed that few scratches appeared on the GO/CF/PEEK coating. As the in vitro cytotoxicity test by murine fibroblast L929 cells shown, the GO/CF/PEEK coating revealed good cytocompatibility. More importantly, GO/CF/PEEK coating exhibited excellent suppression toward Staphylococcus aureus (S. aureus) owing to the antibacterial nature of GO. Therefore, the GO/CF/PEEK composite coated TC4 could be considered as a prospective orthopedic/dental implant material for bone tissue engineering.
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http://dx.doi.org/10.1016/j.jmbbm.2021.104659DOI Listing
June 2021

Synthesis of NaSO mediated cleavable affinity tag for labeling of O-GlcNAc modified proteins via azide-alkyne cycloaddition.

Bioorg Med Chem Lett 2021 Jul 3;48:128244. Epub 2021 Jul 3.

Joint National Laboratory for Antibody Drug Engineering, The First Affiliated Hospital of Henan University, School of Basic Medicine Science, Henan University, 475004 Kaifeng, China. Electronic address:

A facile and convergent procedure for the synthesis of azobenzene-based probe was reported, which could selectively release interested proteins conducted with sodium dithionite. Besides, the cleavage efficiency is closely associated with the structural features, in which an ortho-hydroxyl substituent is necessary for reactivity. In addition, the azobenzene tag applied in the AcGlcNAz-labled proteins demonstrated high efficiency and selectivity in comparison with Biotin-PEG-Alkyne, which provides a useful platform for enrichment of any desired bioorthogonal proteomics.
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http://dx.doi.org/10.1016/j.bmcl.2021.128244DOI Listing
July 2021

Potential therapeutic agents for ischemic white matter damage.

Neurochem Int 2021 Jul 3;149:105116. Epub 2021 Jul 3.

Department of Pharmacology, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, PR China. Electronic address:

Ischemic white matter damage (WMD) is increasingly being considered as one of the major causes of neurological disorders in older adults and preterm infants. The functional consequences of WMD triggers a progressive cognitive decline and dementia particularly in patients with ischemic cerebrovascular diseases. Despite the major stride made in the pathogenesis mechanisms of ischemic WMD in the last century, effective medications are still not available. So, there is an urgent need to explore a promising approach to slow the progression or modify its pathological course. In this review, we discussed the animal models, the pathological mechanisms and the potential therapeutic agents for ischemic WMD. The development in the studies of anti-oxidants, free radical scavengers, anti-inflammatory or anti-apoptotic agents and neurotrophic factors in ischemic WMD were summarized. The agents which either alleviate oligodendrocyte damage or promote its proliferation or differentiation may have potential value for the treatment of ischemic WMD. Moreover, drugs with multifaceted protective activities or a wide therapeutic window may be optimal for clinical translation.
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http://dx.doi.org/10.1016/j.neuint.2021.105116DOI Listing
July 2021

Tumor Immune Microenvironment Components and Checkpoint Molecules in Anaplastic Variant of Diffuse Large B-Cell Lymphoma.

Front Oncol 2021 16;11:638154. Epub 2021 Jun 16.

State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital and School of Basic Medicine, Air Force Medical University, Xi'an, China.

Background: Anaplastic diffuse large B-cell lymphoma(A-DLBCL) is a rare morphological subtype characterized by the presence of polygonal, bizarre-shaped tumor cells. Our previous research found that A-DLBCL displays many genetic alterations and biological features that differ greatly from those of ordinary DLBCL. However, the status of tumor immune microenvironment components and checkpoint molecules in A-DLBCL remains unclear.

Methods: Thirty A-DLBCL patients were enrolled to study tumor immune microenvironment components and checkpoint molecules and their associations with clinicopathological features and prognosis.

Results: Patients with A-DLBCL presented higher expression of PD-L1 (40% 10%, P=0.004) than patients with ordinary DLBCL. FISH analysis showed that extra copies of PD-L1 were more frequent in A-DLBCL cases than in ordinary DLBCL cases (23.3% 4.0%, P=0.001). The numbers of PD-1 TILs (tumor infiltrating lymphocytes) and CD8T cells were significantly lower in A-DLBCL versus ordinary DLBCL. In contrast, the numbers of GATA3 Th2 cells, FOXP3 Tregs and CD33 myeloid-derived suppressor cells (MDSCs) were significantly higher in A-DLBCL than in ordinary DLBCL. The associations between clinicopathological features and tumor immune microenvironment cell frequency were analyzed in A-DLBCL patients. Briefly, the number of PD-1 TILs was lower and the number of CD33 MDSCs was higher in patients with mutated compared to those with wild-type . The number of FOXP3 Tregs was much lower in patients with a noncomplete response (CR) to chemotherapy than in those with a complete response. The number of CD8 T cells showed a decreasing trend in patients with high International Prognostic Index (IPI) scores and in those with concurrent MYC and BCL2 and/or BCL6 abnormalities. Univariate survival analysis showed that patients with PD-L1, mPD-L1(PD-L1 nonmalignant stromal cells) or mPD-L1 status had a significantly poorer overall survival (OS) than those with PD-L1 status. An increase in the number of CD3 T cells, FOXP3 Treg cells and T-bet Th1 cells was significantly associated with prolonged OS in patients with A-DLBCL.

Conclusion: Our study suggests that A-DLBCL displays a distinct pattern of tumor immune microenvironment components and checkpoint molecules that distinguish it from ordinary DLBCL. The analysis of tumor immune microenvironment components and checkpoint molecules could help in predicting the prognosis of A-DLBCL patients and determining therapeutic strategies targeting the tumor immune microenvironment.
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http://dx.doi.org/10.3389/fonc.2021.638154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8242181PMC
June 2021

Hysteresis Modeling and Compensation of Fast Steering Mirrors with Hysteresis Operator Based Back Propagation Neural Networks.

Micromachines (Basel) 2021 Jun 22;12(7). Epub 2021 Jun 22.

School of Astronautics, Harbin Institute of Technology, Harbin 150001, China.

Fast steering mirrors (FSMs), driven by piezoelectric ceramics, are usually used as actuators for high-precision beam control. A FSM generally contains four ceramics that are distributed in a crisscross pattern. The cooperative movement of the two ceramics along one radial direction generates the deflection of the FSM in the same orientation. Unlike the hysteresis nonlinearity of a single piezoelectric ceramic, which is symmetric or asymmetric, the FSM exhibits complex hysteresis characteristics. In this paper, a systematic way of modeling the hysteresis nonlinearity of FSMs is proposed using a Madelung's rules based symmetric hysteresis operator with a cascaded neural network. The hysteresis operator provides a basic hysteresis motion for the FSM. The neural network modifies the basic hysteresis motion to accurately describe the hysteresis nonlinearity of FSMs. The wiping-out and congruency properties of the proposed method are also analyzed. Moreover, the inverse hysteresis model is constructed to reduce the hysteresis nonlinearity of FSMs. The effectiveness of the presented model is validated by experimental results.
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http://dx.doi.org/10.3390/mi12070732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307490PMC
June 2021

The Simple Mix Design Method and Confined Behavior Analysis for Recycled Aggregate Concrete.

Materials (Basel) 2021 Jun 24;14(13). Epub 2021 Jun 24.

State Key Laboratory of Green Building, Western China of Xian University of Architecture & Technology, Xi'an 710055, China.

For the environment protection and sustainable development in building construction, waste concrete can be processed into recycled aggregate to mix the recycled aggregate concrete (RAC). However, the existing mix design methods of RAC were complex, and the mechanical properties of RAC were more weakened than ordinary concrete. This paper presents a simple mix design method for RAC, including orthogonal test and single-factor test. Then, in order to study the behavior of confined RAC, this paper presents a comprehensive experimental study on the RAC filled in steel tube (RCFST) specimens and the RAC filled in GFRP tube (RCFST) specimens. The results show that the proposed mix design method can mix different stable strength grades of RAC promptly and efficiently. In addition, the steel tube and GFRP tube can provide a well confining effect on core RAC to improve the mechanical behavior of column. Moreover, the properties of core RAC in steel tube are the same as the common passive confined concrete, and the properties of core RAC in the GFRP tube are the same as the common active confined concrete. The study results can provide reference for other kinds of RAC mixtures as well as be a foundation for theoretical studies on confined RAC.
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http://dx.doi.org/10.3390/ma14133533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269556PMC
June 2021

Enhanced Efficiency and Stability of All-Inorganic CsPbI Br Perovskite Solar Cells by Organic and Ionic Mixed Passivation.

Adv Sci (Weinh) 2021 Jun 30:e2101367. Epub 2021 Jun 30.

State Key Discipline Laboratory of Wide Band Gap Semiconductor Technology, School of Microelectronics, Xidian University, 2 South Taibai Road, Xi'an, 710071, China.

All-inorganic perovskites have been intensively investigated as potential optoelectronic materials because of their excellent thermal stability, especially for CsPbI Br. Herein, the authors studied the effects of mixed passivation utilizing organic phenylethylammonium bromide and inorganic ionic cesium bromide (PEABr + CsBr) on the all-inorganic perovskite (CsPbI Br) solar cells for the first time. The treatment with different passivation mechanisms enhances the perovskite film quality, resulting in uniform surface morphology and compact film with low trap density. Besides, the passivation improves the energy level alignment, which benefits the hole extraction at the perovskite/HTL interface and drives the interface electron separation, suppressing the charge recombination and realizing a high open-circuit voltage (V ). Finally, the device represents a high power conversion efficiency (PCE) of 16.70%, a V of 1.30 V, and an excellent fill factor (FF) of 0.82. The V loss and high FF should be among the best values for CsPbI Br based devices. Furthermore, the treated devices exhibit remarkable long-term stability with only 8% PCE loss after storing in a glove box for more than 1000 h without encapsulation.
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http://dx.doi.org/10.1002/advs.202101367DOI Listing
June 2021

Joint Microbial and Metabolomic Network Estimation with the Censored Gaussian Graphical Model.

Authors:
Jing Ma

Stat Biosci 2021 Jul 21;13(2):351-372. Epub 2020 Sep 21.

Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

Joint analysis of microbiome and metabolomic data represents an imperative objective as the field moves beyond basic microbiome association studies and turns towards mechanistic and translational investigations. We present a censored Gaussian graphical model framework, where the metabolomic data are treated as continuous and the microbiome data as censored at zero, to identify direct interactions (defined as conditional dependence relationships) between microbial species and metabolites. Simulated examples show that our method metaMint performs favorably compared to the existing ones. metaMint also provides interpretable microbe-metabolite interactions when applied to a bacterial vaginosis data set. R implementation of metaMint is available on GitHub.
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http://dx.doi.org/10.1007/s12561-020-09294-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223740PMC
July 2021

Concurrent expression of enhances antitumor efficacy of oncolytic vaccinia virus but not for Semliki Forest virus.

Mol Ther Oncolytics 2021 Jun 5;21:356-366. Epub 2021 May 5.

Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, 75185, Uppsala, Sweden.

Oncolytic viruses (OVs) represent promising therapeutic agents for cancer therapy by selective oncolysis and induction of anti-tumor immunity. OVs can be engineered to express tumor-associated antigens and immune-modulating agents to provoke stronger antitumor immunity. Here, we engineered vaccinia virus (VV) and Semliki Forest virus (SFV) to express neuroblastoma-associated antigen disialoganglioside (GD2) and the immune modulator neutrophil-activating protein (NAP) and compared their therapeutic potency. Oncolytic VV did not exhibit any antitumor benefits, whereas SFV was able to delay subcutaneous neuroblastoma (NXS2) tumor growth. Additional expression of the GD2 mimotope (GD2m) by VV-GD2m or SFV-GD2m did not improve their anti-tumor capacity compared to the parent viruses. Further arming these OVs with NAP resulted in contrasting anti-tumor efficacy. VV (VV-GD2m-NAP) significantly improved therapeutic efficacy compared to VV-GD2m, which was also associated with a significantly elevated anti-GD2 antibody, whereas there was no additive antitumor efficacy for SFV-GD2m-NAP compared to SFV-GD2m, nor was the anti-GD2 antibody response improved. Instead, NAP induced higher neutralizing antibodies against SFV. These observations suggest that distinct immune stimulation profiles are elicited when the same immunostimulatory factor is expressed by different OVs. Therefore, careful consideration and detailed characterization are needed when engineering OVs with immune-modulators.
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http://dx.doi.org/10.1016/j.omto.2021.04.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182386PMC
June 2021
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