Publications by authors named "Jing Luo"

932 Publications

New Insights into the High-Performance Black Phosphorus Anode for Lithium-Ion Batteries.

Adv Mater 2021 Jul 22:e2101259. Epub 2021 Jul 22.

Department of Mechanical and Materials Engineering, University of Western Ontario, London, Ontario, N6A 5B9, Canada.

Black phosphorus (BP) is a promising anode material in lithium-ion batteries (LIBs) owing to its high electrical conductivity and capacity. However, the huge volume change of BP during cycling induces rapid capacity fading. In addition, the unclear electrochemical mechanism of BP hinders the development of rational designs and preparation of high-performance BP-based anodes. Here, a high-performance nanostructured BP-graphite-carbon nanotubes composite (BP/G/CNTs) synthesized using ball-milling method is reported. The BP/G/CNTs anode delivers a high initial capacity of 1375 mA h g at 0.15 A g and maintains 1031.7 mA h g after 450 cycles. Excellent high-rate performance is demonstrated with a capacity of 508.1 mA h g after 3000 cycles at 2 A g . Moreover, for the first time, direct evidence is provided experimentally to present the electrochemical mechanism of BP anodes with three-step lithiation and delithiation using ex situ X-ray diffraction (XRD), ex situ X-ray absorption spectroscopy (XAS), ex situ X-ray emission spectroscopy, operando XRD, and operando XAS, which reveal the formation of Li P , LiP, and Li P. Furthermore, the study indicates an open-circuit relaxation effect of the electrode with ex situ and operando XAS analyses.
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http://dx.doi.org/10.1002/adma.202101259DOI Listing
July 2021

LncRNA ANRIL Facilitates Vascular Smooth Muscle Cell Proliferation and Suppresses Apoptosis via Modulation of miR-7/FGF2 Pathway in Intracranial Aneurysms.

Neurocrit Care 2021 Jul 20. Epub 2021 Jul 20.

Department of Neurosurgery, First Affiliated Hospital of Anhui Medical University, Hefei City, Anhui Province, China.

Background: Proliferation and apoptosis of vascular smooth muscle cells (VSMCs) are linked to intracranial aneurysm (IA) formation and progression. Long antisense noncoding RNA in the INK4 locus (ANRIL) has been reported to regulate VSMC functions in several cardiovascular diseases. However, little is known about how ANRIL influences VSMC proliferation and apoptosis during IA pathogenesis.

Methods: The expression level of ANRIL in the plasma and arterial wall tissues of patients with IA was detected by real-time quantitative polymerase chain reaction. The functional role of ANRIL in the regulation of VSMC proliferation and apoptosis and its downstream regulatory mechanism were determined using Cell Counting Kit 8, immunofluorescence, terminal-deoxynucleotidyl transferase-mediated UTP nick end labeling, western blotting, luciferase reporter assay, and RNA immunoprecipitation assay.

Results: ANRIL was downregulated in the plasma and arterial wall tissues of patients with IA, when compared with control groups. Overexpression of ANRIL significantly promoted VSMC proliferation and blocked cell apoptosis. Mechanistic studies demonstrated that ANRIL directly bound to microRNA-7 (miR-7) and that overexpression of miR-7 overturned the increased cell proliferation and decreased cell apoptosis, which was induced by ANRIL restoration. Besides, further study showed that ANRIL positively regulated fibroblast growth factor 2 (FGF2) expression via targeting miR-7.

Conclusions: These results suggested that ANRIL affects VSMC proliferation and apoptosis via regulation of the miR-7/FGF2 pathway in IA, which provided a potential novel strategy for the treatment of IA.
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http://dx.doi.org/10.1007/s12028-021-01262-9DOI Listing
July 2021

Curcumin promotes the proliferation, invasion of neural stem cells and formation of neurospheres via activating SDF-1/CXCR4 axis.

Folia Neuropathol 2021 ;59(2):152-160

Ningbo No. 6 Hospital, China, 2the First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, China.

Curcumin is an active ingredient isolated from the rhizomes of Curcuma longa Linn with remarkably non-toxic bioavailability. This is an in vitro study. In this study, we explored the effects of curcumin on the proliferation, migration and neurogenesis of neural stem cells (NSCs). Primary NSCs were isolated from embryonic day 14 rats and then treated with curcumin and/or stromal derived factor-1 (SDF-1). NSCs showed an SDF-1-dependent proliferation and migration. Further results showed that curcumin and SDF-1 both promoted NSCs proliferation, migration and the formation of neurospheres. In addition, Curcumin up-regulated the expression of SDF-1 and promoted the formation of SDF-1/CXCR4 complex in NSCs. The western blot results showed that the phosphorylation levels of ERK, JNK, MAPK, NF-kB and Akt were up-regulated by curcumin. In contrast, the administration of CXCR4 inhibitor AMD3100 could offset the effect of curcumin. These results suggested that curcumin promoted the NSCs proliferation, migration and formation of neurospheres via SDF-1/CXCR4 in NSCs.
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http://dx.doi.org/10.5114/fn.2021.107175DOI Listing
January 2021

Effects of exercise on depressive symptoms among nursing home residents: a meta-analysis.

Aging Ment Health 2021 Jul 20:1-10. Epub 2021 Jul 20.

Trudy Busch Valentine School of Nursing, Saint Louis University, MO, USA.

Objective: We examined the effects of exercise on depression in older adults living in nursing homes and explored the moderator effects of participants, methods, and intervention characteristics.

Methods: We searched 8 databases from inception to January 2020 without date restrictions. We retrieved primary studies measuring exercise with 60-year-olds with depression that were written in English. Two researchers independently coded each primary study and compared codes for discrepancies. They consulted a third researcher to come to consensus. We used random-effects model to compute effect sizes using Hedges' , a forest plot, and and statistics as measures of heterogeneity. We also examined moderator analyses.

Results: Twenty-one studies included 2,594 participants (81.8 ± 4.8 years old). Overall, exercise showed a small improvement in depression compared to controls (ES=.25; 95%CI 0.11, 0.38; =.000). Interestingly, interventionists by physiotherapists had a smaller effect on depressive symptoms (.04) than other health interventionists (.37). As people aged, exercise was less effective in reducing depression (slope=-.03, Q=5.03, =.025). Researchers who included a higher percentage of women in exercise showed less improvement of depression (=-.01, Q=8.60, =.003). Also, when researchers used depression scales with higher reliability values, they measured higher levels of depression (slope = 5.48, Q=5.60, =.018). No other quality indicators moderated the effects of exercise on depression.

Conclusion: Exercise significantly improved depressive symptoms among older adults in nursing homes. Exercise might be used as adjunct/alternative complementary treatment for improving depressive symptoms in older adults in nursing homes.
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http://dx.doi.org/10.1080/13607863.2021.1951658DOI Listing
July 2021

The metabolic landscape of decidua in recurrent pregnancy loss using a global metabolomics approach.

Placenta 2021 Jul 8;112:45-53. Epub 2021 Jul 8.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China. Electronic address:

Introduction: Maternal metabolism undergoes dynamic changes during pregnancy. A deviation from this physiological metabolic status might be involved in the pathogenesis of pregnancy complications, such as recurrent pregnancy loss (RPL). Decidua is an important uterine tissue, which provides immunological protection as well as nutritional support to the developing embryo during early pregnancy. Previous studies have shown that aberrant metabolism of the decidua is related to the etiology of RPL. However, the metabolic profile of the decidua in RPL has not yet been fully elucidated.

Methods: In the current study, decidual samples from RPL patients (n = 23) and normal pregnancy (NP) women (n = 30) were collected, and hydrophilic and hydrophobic metabolites were extracted and measured using a liquid chromatography electrospray ionization tandem mass spectrometry system. Besides, the mRNA expression of several critical enzymes involved in sphingolipid metabolism and glycerophospholipid metabolism was detected.

Results: The OSC-PLS-DA scores plot illustrates that metabolic differences are present in the decidual tissue of RPL patients compared with that of NP women. Combining multivariate analysis with univariate statistical analysis, a total of 62 metabolites related to RPL were selected, including carnitine, glycerophospholipids, sphingomyelin (SM), ceramide, organic acids and their derivatives, and amino acid metabolomics. KEGG analysis showed that abnormalities in multiple metabolic pathways occurred in RPL decidua, including vitamin digestion and absorption, tryptophan metabolism, citrate cycle, arginine biosynthesis, glycerophospholipid metabolism, sphingolipid metabolism, and sphingolipid signaling pathway. Increased SM synthase and decreased Phospholipase A2 Group IIE mRNA levels were detected in RPL compared with NP group.

Discussion: Disruption of decidual metabolism, especially glycerophospholipid metabolism and sphingolipid metabolism, might be involved in the occurrence of RPL.
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http://dx.doi.org/10.1016/j.placenta.2021.07.001DOI Listing
July 2021

The Roles of General and Domain-Specific Perceived Stress in Healthy Aging.

J Gerontol B Psychol Sci Soc Sci 2021 Jul 15. Epub 2021 Jul 15.

Department of Psychology, University of Illinois at Urbana-Champaign, 603 E. Daniel St., Champaign, IL, USA.

Objectives: Theoretical and empirical evidence suggests the existence of a general perceived stress factor overarching different life domains. The present study investigated the general perceived stress relative to domain-specific perceived stress as predictors of 26 diverse health outcomes, including mental and physical health, health behaviors, cognitive functioning, and physiological indicators of health.

Method: A bifactor exploratory structural equational modelling (BiESEM) approach was adopted in two aging samples from the Health and Retirement Study (HRS; N = 8325 in Sample 1, N = 7408 in Sample 2).

Results: Across the two samples, perceived stress was well-represented by a bifactor structure where there was a robust general perceived stress factor representing a general propensity towards stress perception. Meanwhile, after controlling for the general perceived stress factor, specific factors that represent perceived stress in different life domains were still clearly present. Results also suggested age, sex, race, education, personality traits, and past and recent stressor exposure as possible factors underlying individual differences in the general perceived stress factor. The general perceived stress factor was the most robust predictor of the majority of health outcomes, as well as changes in mental health outcomes. The specific factor of perceived neighborhood stress demonstrated incremental predictive effects across different types of health outcomes.

Discussion: The current study provides strong evidence for the existence of a general perceived stress factor that captures variance shared among stress across life domains, and the general perceived stress factor demonstrated substantial prospective predictive effects on diverse health outcomes in older adulthood.
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http://dx.doi.org/10.1093/geronb/gbab134DOI Listing
July 2021

Apolipoprotein E ε4 and ε3 alleles associate with cerebrospinal fluid tau and cognition in the presence of amyloid-β in mild cognitive impairment but not in Alzheimer's disease.

J Integr Neurosci 2021 Jun;20(2):277-286

Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, 400016 Chongqing, China.

Apolipoprotein E is the most well-established genetic risk factor for Alzheimer's disease. However, the associations of apolipoprotein E with tau pathology and cognition remain controversial. The research checks the hypothesis that the relationships between apolipoprotein E alleles and cerebrospinal fluid tau and cognition differ in persons with and without significant amyloid-β deposition. We divided 1119 subjects into cognitively normal (n = 275), mild cognitive impairment (n = 629), and Alzheimer's disease (n = 215), and these subjects were from the Alzheimer's Disease Neuroimaging Initiative database. Linear regression models were used to compare the relationships of apolipoprotein E alleles with cerebrospinal fluid tau and cognition in persons with significant amyloid-β deposition relative to individuals without significant amyloid-β deposition. The associations of apolipoprotein E ε4 and ε3 with total tau (T-tau), phosphorylated tau (P-tau), and Alzheimer's disease assessment scale was significantly substantial among participants with significant amyloid-β deposition. Stratified analyses showed that apolipoprotein E ε4 related to increased concentrations of T-tau, P-tau, and Alzheimer's disease assessment scale and apolipoprotein E ε3 associated with decreased concentrations of T-tau, P-tau, and Alzheimer's disease assessment scale in mild cognitive impairment participants with significant amyloid-β deposition, but not in Alzheimer's disease. Our study shows that the presence of apolipoprotein E ε4 and ε3 alleles is related to tau pathology and cognitive impairment in the presence of amyloid-β in mild cognitive impairment, but not in Alzheimer's disease. This work indirectly provides additional evidence that apolipoprotein E and amyloid-β may not have a role in modulating clinical Alzheimer's disease, and apolipoprotein E ε3 may be supposed to be protective to mild cognitive impairment.
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http://dx.doi.org/10.31083/j.jin2002027DOI Listing
June 2021

Facial Numbness, Dysarthria, Muscle Atrophy, and Weakness in a Young Patient.

JAMA Neurol 2021 Jul 12. Epub 2021 Jul 12.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing, China.

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http://dx.doi.org/10.1001/jamaneurol.2021.2136DOI Listing
July 2021

Cytoprotective effects of spleen-invigorating pill against 5-fluorouracil injury to mouse bone marrow stromal cells.

J Ethnopharmacol 2021 Jul 8;280:114397. Epub 2021 Jul 8.

School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China. Electronic address:

Ethnopharmacological Relevance: Spleen-invigorating pills (SIP) are composed of Codonopsis, fried Atractylodes, tangerine peel, Fructus aurantii immaturus (fried), fried hawthorn, and colored malt. SIP strengthens the spleen and increases appetite and is often used as a chemotherapy adjuvant.

Aim Of The Study: We aimed to explore the protective effects and mechanism of action for SIP on mouse bone marrow stromal cells (OP9) injured by 5-fluorouracil (5-FU).

Materials And Methods: The effects of SIP on OP9 cells injured by 5-FU were evaluated, and high-performance liquid chromatography (HPLC) was used as a quality control method. The experiments were divided into a control group, a model group, an epidermal growth factor (EGF) treatment group, and an SIP treatment group. The cell survival rate, apoptotic cell morphology, cell apoptosis rate, and the contents of caspase 3 were evaluated to determine the protective effects of SIP in OP9 cells injured by 5-FU. Network pharmacology was used to predict the mechanism through which SIP mediates anti-chemotherapy damage. The nitric oxide (NO) and nitric oxide synthase (iNOS) levels and the expression of nuclear factor erythroid-2 related factor 2 (Nrf2) and p62 protein were detected to explore the mechanism through which SIP mediates anti-chemotherapy damage through the regulation of oxidative stress.

Results: Cell counting kit-8 (CCK8) detection showed that 5-FU reduced OP9 cell survival, and SIP blocked the inhibition of OP9 cell growth induced by 5-FU. When OP9 cells were treated with both SIP (10 g L) and 5-FU (2.5 × 10 g L) for 24 h, compared with the model group, the early apoptosis rates significantly decreased, and the activity of caspase 3 was significantly reduced. The results of network pharmacology and Western blot showed that compared with the model group, in the SIP group, the NO levels decreased, iNOS release decreased, and the expression of Nrf2 and p62 proteins increased.

Conclusion: The protective effects of SIP on OP9 cells injured by 5-FU were significant. SIP may play a cytoprotective role by mediating changes in oxidative stress-related proteins. The specific mechanism of action through which SIP mediates these effects remains to be further studied.
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http://dx.doi.org/10.1016/j.jep.2021.114397DOI Listing
July 2021

SMRT and Illumina RNA Sequencing and Characterization of a Key Gene during the Flower Senescence in 'Siberia'.

Genes (Basel) 2021 Jun 6;12(6). Epub 2021 Jun 6.

Key Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.

Lily ( spp.) is an important cut flower around the world. Flower senescence in lilies is characterized by the wilting and abscission of tepals, which results in a decrease in flower quality and huge economic loss. However, the mechanism underlying flower senescence in lilies is largely unknown. In this study, single-molecule, real-time (SMRT) and Illumina sequencing were carried out in 'Siberia'. Sequencing yielded 73,218 non-redundant transcripts, with an N50 of 3792 bp. These data were further integrated with three published transcriptomes through cogent analysis, which yielded 62,960 transcripts, with an increase in N50 of 3935 bp. Analysis of differentially expressed genes showed that 319 transcription factors were highly upregulated during flower senescence. The expression of twelve genes and eleven senescence-associated genes () showed that and were highly expressed in senescent flowers. Transient overexpression of and in tepals of lily notably accelerated flower senescence, and the promoter activity of was strongly induced by . This work supported new evidence for the molecular mechanism of flower senescence and provided better sequence data for further study in lilies.
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http://dx.doi.org/10.3390/genes12060869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227295PMC
June 2021

Detection of the Microvascular Changes of Diabetic Retinopathy Progression Using Optical Coherence Tomography Angiography.

Transl Vis Sci Technol 2021 06;10(7):31

Department of Ophthalmology, the Second Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.

Purpose: To investigate microvascular parameters that are related to the severity of diabetic retinopathy (DR) with optical coherence tomography angiography (OCTA).

Methods: In total, 105 eyes from 105 diabetic patients were recruited in this prospective cross-sectional study, including 37 eyes with no clinical signs of DR (NoDR), 43 eyes with nonproliferative diabetic retinopathy (NPDR), and 25 eyes with proliferative diabetic retinopathy (PDR). Angiogram images from the parafoveal superficial capillary plexus (SCP), the deep capillary plexus (DCP), and the radial peripapillary capillary plexus were analyzed, and metrics were compared among groups. Multivariate regression analysis was used to identify the best OCTA parameters that could distinguish DR severity among groups.

Results: Parafoveal vessel diameter index in the SCP and vessel density (VD) in the DCP showed the strongest correlation with the severity of DR (P < 0.01). Extrafoveal avascular area in the SCP was the parameter that could most distinguish NoDR from NPDR (P < 0.01) with sensitivity and specificity of 83.72% and 78.38%, respectively. VD in the DCP also was the most sensitive biomarker to distinguish NPDR from PDR (P < 0.01) with sensitivity and specificity of 84.00% and 79.07%, respectively.

Conclusions: The microvascular changes in the SCP and DCP in DR may have different characteristics that could be identified with specific OCTA parameters. OCTA serves as a promising technology to discriminate eyes with different severity of DR.

Translational Relevance: Our study investigated OCTA metrics and severity of DR. At different stages of DR, ophthalmologists may focus on specific OCTA parameters to predict the progression of retinopathy in individual patients.
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http://dx.doi.org/10.1167/tvst.10.7.31DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254014PMC
June 2021

Redesign of urban biowaste sustainable management system based on industrial ecology concept: A case study in China.

Sci Total Environ 2021 Jun 10;793:148425. Epub 2021 Jun 10.

Resource & Environment Industry Department, China International Engineering Consulting Corporation, Beijing 10084, China. Electronic address:

Urban biowaste, which refers to the organic fraction of municipal solid waste (MSW), is a predominant type of waste in low- and middle-income countries. Therefore, the sustainable management of urban biowaste is a key problem in solid waste management (SWM). However, treatment technologies usually come with secondary pollution and by-products that need further treatment, which would limit the economic and environmental benefits of the waste management system. The concept of industrial ecology has the potential to be introduced into the waste management field to solve this problem. Based on a practical case study of a city in eastern China, this paper conducted a complete urban biowaste treatment system planning exercise using concepts in industrial ecology. The establishment of the new system has significant economic, environmental and social benefits. The total output value of the project would reach 342 million yuan in 2025, corresponding to 945,000 tons of solid wastes consumed every year, which would significantly reduce the environmental pollution caused by solid waste. More than 700 jobs could also be created. In addition, the establishment of an urban biowaste sustainable management system requires the joint effort of enterprise, government and the public, which in turn requires innovation in business models and management policies. Therefore, a special management committee should be established to promote collaboration among stakeholders, and an online platform that enables end-to-end process supervision covering the entire system, including emergency management mechanisms, needs to be established to ensure the long-term stable operation of various treatment facilities. The results of this paper show that synergies among technologies should be promoted to provide systemic benefit, and that the infrastructure for pollution control should be shared to ensure high utilization.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148425DOI Listing
June 2021

Does High-Speed Rail Opening Affect the Health Care Environment?-Evidence From China.

Front Public Health 2021 9;9:708527. Epub 2021 Jun 9.

School of Economics, Shandong Normal University, Jinan, China.

Using the panel data of 280 prefecture-level cities in China from 2004 to 2014, this paper examines the effects of high-speed rail opening on health care environment based on Difference-in-Differences method (DID). Through an empirical analysis, the results proved that high-speed rail opening can significantly promote the health care environment and this effect is different in regions with different levels of economic development. Finally, we tested the mechanisms of how the high-speed rail opening affects the healthcare environment. High-speed rail opening improves the healthcare environment by increasing road accessibility and promoting economic development. Our results support the view that high-speed rail opening has an important contribution to the improvement of health care conditions.
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http://dx.doi.org/10.3389/fpubh.2021.708527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219950PMC
June 2021

Recyclable self-floating A-GUN-coated foam as effective visible-light-driven photocatalyst for inactivation of Microcystis aeruginosa.

J Hazard Mater 2021 Jun 15;419:126407. Epub 2021 Jun 15.

College of Civil Engineering, Fuzhou University, 350116 Fujian, China; State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, 350002 Fujian, China; Fujian Provincial Key Laboratory of Electrochemical Energy Storage Materials, Fuzhou University, 350002 Fujian, China. Electronic address:

In this work, a recyclable self-floating A-GUN-coated (Ag/[email protected]@UIO-66(NH)-coated) foam was fabricated for effective inactivation of Microcystis aeruginosa (M. aeruginosa) under visible light. The floating photocatalyst was able to inactivate 98% of M. aeruginosa within 180 min under the visible-light irrigation, and the floating photocatalyst exhibited a stable performance in various conditions. Moreover, the inactivation efficiency can still maintain nearly 92% after five times recycle experiments, showing excellent photocatalytic stability. Furthermore, effects of A-GUN/SMF floating catalyst on the physiological properties, cellular organics, and algal functional groups of M. aeruginosa were studied. The floating photocatalyst can not only make full use of excellent photocatalytic activities of A-GUN nanocomposite, but also promote contact between catalyst and algae, and realize the effective recovery of the photocatalyst. Finally, possible photocatalytic inactivation mechanisms of algae were obtained, which provides references for removing cyanobacteria blooms in real water bodies.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126407DOI Listing
June 2021

Enhanced insightfulness and neural activation induced by metaphorical solutions to appropriate mental distress problems.

Psychophysiology 2021 Jun 25:e13886. Epub 2021 Jun 25.

Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences (CAS), Beijing, P.R.China.

Although the neural correlates of novelty and appropriateness of creative insight during cognitive tasks have been investigated in several studies, they have not been examined during mental distress in a psychotherapeutic setting. This study aimed to reveal the promoting effects of novelty and appropriateness processing on therapeutic insight in a micro-psychotherapeutic setting. We examined the effects of appropriateness (between-subject factor: appropriateness group, 20 participants; inappropriateness group, 21 participants) by manipulating the preceding negative scenarios that either fit or did not fit the subsequent solutions, and those of novelty (within-subject factor) by varying the linguistic expressions for describing solutions (metaphorical, literal, or problem-restatement). Event-related functional magnetic resonance images were collected. We found the following effects: an interactive effect of the two factors on insightfulness and activation in the bilateral hippocampus and amygdala, right superior frontal gyrus, and left superior/middle temporal gyrus; a simple effect of novelty on activation in the bilateral inferior frontal gyrus, fusiform gyrus, and inferior/middle occipital gyrus; and a simple effect of appropriateness on activation in the left inferior parietal lobule. Our findings indicate that solutions with high novelty and appropriateness generate the highest levels of therapeutic insightfulness as well as the strongest activation in the hippocampus and amygdala, which may be involved in episodic memory encoding.
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http://dx.doi.org/10.1111/psyp.13886DOI Listing
June 2021

Pyruvate kinase M2 mediates fibroblast proliferation to promote tubular epithelial cell survival in acute kidney injury.

FASEB J 2021 07;35(7):e21706

Center for Kidney Disease, The Second Affiliated Hospital, Nanjing Medical University, Nanjing, China.

Acute kidney injury (AKI) is a devastating condition with high morbidity and mortality rates. The pathological features of AKI are tubular injury, infiltration of inflammatory cells, and impaired vascular integrity. Pyruvate kinase is the final rate-limiting enzyme in the glycolysis pathway. We previously showed that pyruvate kinase M2 (PKM2) plays an important role in regulating the glycolytic reprogramming of fibroblasts in renal interstitial fibrosis. The present study aimed to determine the role of PKM2 in fibroblast activation during the pathogenesis of AKI. We found increased numbers of S100A4 positive cells expressing PKM2 in renal tissues from mice with AKI induced via folic acid or ischemia/reperfusion (I/R). The loss of PKM2 in fibroblasts impaired fibroblast proliferation and promoted tubular epithelial cell death including apoptosis, necroptosis, and ferroptosis. Mechanistically, fibroblasts produced less hepatocyte growth factor (HGF) in response to a loss of PKM2. Moreover, in two AKI mouse models, fibroblast-specific deletion of PKM2 blocked HGF signal activation and aggravated AKI after it was induced in mice via ischemia or folic acid. Fibroblast proliferation mediated by PKM2 elicits pro-survival signals that repress tubular cell death and may help to prevent AKI progression. Fibroblast activation mediated by PKM2 in AKI suggests that targeting PKM2 expression could be a novel strategy for treating AKI.
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http://dx.doi.org/10.1096/fj.202100040RDOI Listing
July 2021

Rose (Rosa hybrida) Ethylene Responsive Factor 3 Promotes Rose Flower Senescence via Direct Activation of The Abscisic Acid Synthesis-Related 9-CIS-EPOXYCAROTENOID DIOXYGENASE Gene.

Plant Cell Physiol 2021 Jun 22. Epub 2021 Jun 22.

Key Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

During plant senescence, energy and nutrients are transferred to young leaves, fruits or seeds. However, senescence reduces flower quality, which leads to huge economic losses in flower production. Ethylene is an important factor affecting the quality of cut roses during transportation and storage. Ethylene-responsive factors (ERFs) are key nodes in ethylene signaling, but the molecular mechanism underlying ERFs regulated flower senescence is not well understood. We addressed this issue in the present study by focusing on RhERF3 from Rosa hybrida, an ERF identified in a previous transcriptome analysis of ethylene-treated rose flowers. Expression of RhERF3 was strongly induced by ethylene during rose flower senescence. Transient silencing of RhERF3 delayed flower senescence whereas overexpression (OE) accelerated the process. RNA sequencing analysis of RhERF3 OE and pSuper vector control samples identified 13,214 differentially expressed genes that were mostly related to metabolic process and plant hormone signal transduction. Transient activation and yeast one hybrid assays demonstrated that RhERF3 directly bound the promoter of the 9-cis-epoxycarotenoid dioxygenase (RhNCED1) gene and activated gene expression. Thus, a RhERF3/RhNCED1 axis accelerates rose flower senescence.
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http://dx.doi.org/10.1093/pcp/pcab085DOI Listing
June 2021

lncRNA GAS6-AS1 inhibits progression and glucose metabolism reprogramming in LUAD via repressing E2F1-mediated transcription of GLUT1.

Mol Ther Nucleic Acids 2021 Sep 1;25:11-24. Epub 2021 May 1.

Department of Thoracic Surgery, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing 210000, China.

Glucose metabolism reprogramming is one of the hallmarks of cancer cells, although functional and regulatory mechanisms of long noncoding RNA (lncRNA) in the contribution of glucose metabolism in lung adenocarcinoma (LUAD) remain incompletely understood. The aim of this study was to uncover the role of GAS6-AS1 in the regulation of progression and glucose metabolism in LUAD. We discovered that overexpression of GAS6-AS1 suppressed tumor progression of LUAD both and . Metabolism-related assays revealed that GAS6-AS1 inhibited glucose metabolism reprogramming. Mechanically, GAS6-AS1 was found to repress the expression of glucose transporter GLUT1, a key regulator of glucose metabolism. Ectopic expression of GLUT1 restored the inhibition effect of GAS6-AS1 on cancer progression and glucose metabolism reprogramming. Further investigation identified that GAS6-AS1 directly interacted with transcription factor E2F1 and suppressed E2F1-mediated transcription of GLUT1, and GAS6-AS1 was downregulated in LUAD tissues and correlated with clinicopathological characteristics and survival of patients. Taken together, our results identified GAS6-AS1 as a novel tumor suppressor in LUAD and unraveled its underlying molecular mechanism in reprogramming glucose metabolism. GAS6-AS1 potentially may serve as a prognostic marker and therapeutic target in LUAD.
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http://dx.doi.org/10.1016/j.omtn.2021.04.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181633PMC
September 2021

Mussel-Inspired Immobilization of Photocatalysts with Synergistic Photocatalytic-Photothermal Performance for Water Remediation.

ACS Appl Mater Interfaces 2021 Jul 17;13(26):31066-31076. Epub 2021 Jun 17.

Shanghai Urban Construction Design and Research Institute Co., Ltd., 200125 Shanghai, China.

The serious problem of pharmaceutical and personal care product pollution places great pressure on aquatic environments and human health. Herein, a novel coating photocatalyst was synthesized by adhering Ag-AgCl/WO/g-CN (AWC) nanoparticles on a polydopamine (PDA)-modified melamine sponge (MS) through a facile layer-by-layer assembly method to degrade trimethoprim (TMP). The formed PDA coating was used for the anchoring of nanoparticles, photothermal conversion, and hydrophilic modification. TMP (99.9%; 4 mg/L) was removed in 90 min by the photocatalyst coating (AWC/PDA/MS) under visible light via a synergistic photocatalytic-photothermal performance route. The stability and reusability of the AWC/PDA/MS have been proved by cyclic experiments, in which the removal efficiency of TMP was still more than 90% after five consecutive cycles with a very little mass loss. Quantitative structure-activity relationship analysis revealed that the ecotoxicities of the generated intermediates were lower than those of TMP. Furthermore, the solution matrix effects on the photocatalytic removal efficiency were investigated, and the results revealed that the AWC/PDA/MS still maintained excellent photocatalytic degradation efficiency in several actual water and simulated water matrices. This work develops recyclable photocatalysts for the potential application in the field of water remediation.
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http://dx.doi.org/10.1021/acsami.1c02973DOI Listing
July 2021

Silencing the gene decreases the proliferation, migration and invasion of human triple-negative breast cancer cells.

Exp Ther Med 2021 Aug 24;22(2):792. Epub 2021 May 24.

Department of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.

Triple-negative breast cancer (TNBC) is highly invasive, has a high rate of recurrence and is associated with a poor clinical outcome when compared with non-TNBC due to a lack of effective and targeted treatments. The coatomer protein complex subunit β2 (COPB2) is upregulated in various types of malignant cancer. The present study demonstrated that COPB2 expression levels were significantly upregulated in breast carcinoma HS-578T cells (clonal cells originating from TNBC) when compared with non-TNBC MCF-7 cells. HS-578T cells also exhibited higher rates of proliferation, invasion and transendothelial migration when compared with MCF-7 cells. Moreover, it was identified that genetically silencing the gene using a lentivirus-short hairpin RNA inhibited the proliferative, colony formation, migratory and invasive properties of the TNBC HS-578T cells. Mediation of the silencing effect may be associated with regulating the phosphorylation of serine/threonine kinase AKT in the PI3K/AKT signaling pathway. These results suggested the importance of COPB2 in promoting the proliferation of TNBC cells and identified COPB2 as a potential novel therapeutic target.
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http://dx.doi.org/10.3892/etm.2021.10224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170640PMC
August 2021

A coordinated analysis of the associations among personality traits, cognitive decline, and dementia in older adulthood.

J Res Pers 2021 Jun 23;92. Epub 2021 Apr 23.

Northwestern University.

There are individual differences in the rates of cognitive decline across later adulthood. Personality traits are among the factors that may account for these differences. The current project investigated whether personality traits were associated with trajectories of cognitive decline, and whether the associations were different before and after dementia diagnosis. The data was analyzed using linear mixed effects regression. Across study aims is a focus on replicability and generalizability. Each question was addressed in four independent longitudinal studies (EAS, MAP, ROS, SATSA), then meta-analyzed, providing estimates of replicability. Results indicated that low neuroticism and high openness were associated with total cognitive function. We detected evidence for cognitive decline in all four samples, and openness was associated with decline post dementia diagnosis.
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http://dx.doi.org/10.1016/j.jrp.2021.104100DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168939PMC
June 2021

CmNAC73 Mediates the Formation of Green Color in Chrysanthemum Flowers by Directly Activating the Expression of Chlorophyll Biosynthesis Genes and .

Genes (Basel) 2021 May 8;12(5). Epub 2021 May 8.

Key Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.

Chrysanthemum is one of the most beautiful and popular flowers in the world, and the flower color is an important ornamental trait of chrysanthemum. Compared with other flower colors, green flowers are relatively rare. The formation of green flower color is attributed to the accumulation of chlorophyll; however, the regulatory mechanism of chlorophyll metabolism in chrysanthemum with green flowers remains largely unknown. In this study, we performed Illumina RNA sequencing on three chrysanthemum materials, and cultivars 'Chunxiao' and 'Green anna', which produce white, light green and dark green flowers, respectively. Based on the results of comparative transcriptome analysis, a gene encoding a novel NAC family transcription factor, CmNAC73, was found to be highly correlated to chlorophyll accumulation in the outer whorl of ray florets in chrysanthemum. The results of transient overexpression in chrysanthemum leaves showed that CmNAC73 acts as a positive regulator of chlorophyll biosynthesis. Furthermore, transactivation and yeast one-hybrid assays indicated that CmNAC73 directly binds to the promoters of chlorophyll synthesis-related genes and . Thus, this study uncovers the transcriptional regulation of chlorophyll synthesis-related genes and by CmNAC73 and provides new insights into the development of green flower color in chrysanthemum and chlorophyll metabolism in plants.
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http://dx.doi.org/10.3390/genes12050704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151904PMC
May 2021

Using remote consultation to enhance diagnostic accuracy of bedside transthoracic echocardiography during COVID-19 pandemic.

Echocardiography 2021 May 31. Epub 2021 May 31.

Department of Ultrasonography, Public Health Clinical Center of Chengdu, Chengdu, China.

Objective: The aim of this study was to evaluate the clinical significance of remote consultation over bedside transthoracic echocardiography (RC-B-TTE) for patients with coronavirus disease 2019 (COVID-19).

Methods: Five frontline echocardiographers performed and interpreted B-TTE for 30 patients with COVID-19 in the isolation wards, and the on-site B-TTE reports (OSR) were generated. Then remote consultation over the 30 B-TTE studies was conducted by two experienced echocardiographic consultants while blinded to the OSR, and the corresponding remote consultation reports (RCR) were generated. Subsequently, the five frontline echocardiographers were convened together to discuss the difference between the OSR and RCR, and to confirm the correct interpretation and the misdiagnosis using a "majority-vote" consensus as the diagnostic "gold standard". Afterwards the reasons for the misdiagnosis were given by the frontline echocardiographers themselves. The inter-rater agreement between the OSR and the "gold standard" was assessed using Kappa coefficient and percent agreement.

Results: Complete correctness of the 30 copies of the RCR were determined by the 5 frontline echocardiographers. The reliability of the OSR in the findings of cardiac chamber dilation, left ventricular hypertrophy and pulmonary hypertension were weak (Kappa <0.6). The reliability of the OSR in the recognition of major cardiac abnormalities was very weak (Kappa =0.304, percent agreement =63.3%). Misdiagnosis of major abnormalities was found in 11 copies of OSR (11/30, 36.7%).

Conclusions: The protocol of RC-B-TTE has shown noticeable superiority in ameliorating diagnostic accuracy of echocardiography, which should be generalized to clinical practice during the COVID-19 or similar pandemic.
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http://dx.doi.org/10.1111/echo.15124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239538PMC
May 2021

Preparation of dual-chamber microcapsule by Pickering emulsion for self-healing application with ultra-high healing efficiency.

J Colloid Interface Sci 2021 Oct 18;600:660-669. Epub 2021 May 18.

Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, PR China.

This work presented a new concept for designing dual-chamber self-healing microcapsules, which encapsulated both healing and curing species within a single microcapsule via Pickering emulsion photopolymerization. In our strategy, robust SiO spheres encapsulating curing agent were firstly synthesized and used as Pickering emulsifiers to prepare emulsions loaded with self-healing agent and photo-curable monomer. Upon exposure to UV light, the photo-curable monomer underwent photo-crosslinking and converted into microcapsules wall. In the meanwhile, the SiO spheres encapsulating curing agent were trapped in the microcapsule wall. The dual-chamber microcapsule which loaded the healing agent in its core and curing agent within its shell, was thus prepared. The presence of both the encapsulated healing and curing agent within a single capsule was demonstrated by infrared spectrometry and thermogravimetric analysis. Upon fracture, the healing agent and curing agent are released simultaneously from the dual-chamber microcapsule, which facilitates the interaction of the two agents, and enhances the healing efficiency. Up to 85% healing efficiency of the epoxy resin was achieved in 1 h, which was much higher than that of the traditional double microcapsules self-healing system (65%), demonstrating the excellent self-healing performance of the dual-chamber microcapsules. It has been demonstrated that the coating based on dual-chamber microcapsule presented reliable and outstanding self-healing anti-corrosion efficiency. By changing the species of healing agent, curing agent and wall substances (photo-curable monomer), the as-prepared dual-chamber microcapsules can meet different requirements of versatile self-healing system.
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http://dx.doi.org/10.1016/j.jcis.2021.05.066DOI Listing
October 2021

Effects of Low-Frequency Repetitive Transcranial Magnetic Stimulation on Language Recovery in Poststroke Survivors With Aphasia: An Updated Meta-analysis.

Neurorehabil Neural Repair 2021 May 25:15459683211011230. Epub 2021 May 25.

Department of Rehabilitation Medicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

The effects of low-frequency repetitive transcranial magnetic stimulation (LF-rTMS) on treating poststroke aphasia (PSA) remain inconclusive. We aimed to evaluate the efficacy and safety of LF-rTMS on language function poststroke and determine potential factors that may affect treatment effects. Electronic databases, including MEDLINE, EMBASE, and Cochrane Library were searched to identify relevant randomized controlled trials (RCTs) concerning the effects of LF-rTMS on language performance poststroke. We adopted fixed- and random-effects models to estimate intervention effects, which were represented by the Hedges' and 95% CIs. Subgroup analyses regarding several factors potentially influencing the effects of LF-rTMS on language recovery were also conducted. A total of 14 RCTs involving 374 participants were included in the meta-analysis. The pooled analysis showed the positive and significant effects of LF-rTMS on language function, both short-term (Hedges' = 0.65; < .05) and long-term (Hedges' = 0.46; < .05). Subgroup analyses demonstrated that LF-rTMS for 20 minutes per day over 10 days yielded the largest effect size (Hedges' = 1.02; < .05) and that LF-rTMS significantly improved language performance in the chronic stage after stroke (Hedges' = 0.55; < .05). Patients with different native languages might have diverse responses to LF-rTMS treatment efficacy. Additionally, there were significant improvements in language subtests, including naming, repetition, comprehension, and writing. Overall, this updated meta-analysis demonstrated that LF-rTMS has significant positive effects on PSA, with moderate treatment effects. It provides additional evidence to support LF-rTMS as a promising complementary therapy to promote language recovery in PSA.
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http://dx.doi.org/10.1177/15459683211011230DOI Listing
May 2021

Elucidating the nature of linguistic processing in insight.

Psych J 2021 May 24. Epub 2021 May 24.

Beijing Key Laboratory of Learning and Cognition, School of Psychology, Capital Normal University, Beijing, China.

The relationship between language and thinking has long been a matter of debate and a research focus in studies on thinking and problem solving, including creativity. Previous behavioral studies have found that verbalization of one's internal thoughts does not participate in or even interfere with the creative insight process, thus suggesting that insight may take place nonverbally. In contrast to this hypothesis, the present study proposes a new one. That is, given that the basic categories or fundamental functions of key concepts or objects are critically changed or expanded during insightful thinking, the linguistic processing accompanying insight can be reflected as category-related representation and recategorization processes, which can be critically mediated by the posterior middle temporal gyrus and the angular gyrus (pMTG/AG). Using constraint-relaxation insight riddles as materials in a guided-insight experimental design with external hints to trigger the insightful representational change, this preliminary neuroimaging study of 11 participants found the involvement of pMTG/AG during moments of induced insight, but did not find the activation of left ventral frontal areas which are typically involved in verbalizing of one's internal thoughts. Although this observation still cannot exclude the possibility of internal verbalization in insightful restructuring, it implies that linguistic processing in insight may take the more fundamental form of category-related processing.
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http://dx.doi.org/10.1002/pchj.456DOI Listing
May 2021

The low-field NMR studies the change in cellular water in tilapia fillet tissue during different drying conditions.

Food Sci Nutr 2021 May 11;9(5):2644-2657. Epub 2021 Mar 11.

College of Mechanical and Power Engineering Guangdong Ocean University Zhanjiang China.

The muscle is a highly organized tissue, where there are three different moistures including free water, entrapped water, and bound water. These moistures were distributed in intercellular spaces, intracellular spaces, and other solute environments, respectively. Understanding the moisture migration in different environments is crucial to enhance energy efficiency and improve the quality of processed food. Therefore, the tilapia fillets were used to experiment, and the low-field nuclear magnetic resonance technique is used to measure the change in different moistures during the drying process. The study found that free water is the highest when cell membranes started to rupture. In addition, it also observed that the cell membrane ruptures at different stages of drying. The result of this study provides critical information that could be used to guide the study of the dynamic mechanisms underlying drying and the development of drying technology for tilapia fillets and similar aquatic products.
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http://dx.doi.org/10.1002/fsn3.2221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116849PMC
May 2021

Predictive value of postoperative C-reactive protein-to-albumin ratio in anastomotic leakage after esophagectomy.

J Cardiothorac Surg 2021 May 17;16(1):133. Epub 2021 May 17.

Department of Cardiothoracic Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Introduction: Among the many possible postoperative complications, anastomotic leakage (AL) is the most common and serious. Therefore, the purpose of this study was to explore the ability of various inflammatory and nutritional markers to predict postoperative AL in patients after esophagectomy.

Methods: A total of 273 patients were retrospectively evaluated and enrolled into this study. Perioperative, surgery-related, tumor-related and laboratory tests data were extracted and analyzed. The discriminatory ability and optimal cut-off value was evaluated according to the receiver operating characteristic (ROC) curve analysis. Univariate and multivariate analyses were performed to access the potential risk factors for AL.

Results: The overall incidence of AL was 12.5% (34/273). C-reactive protein-to-albumin ratio (CRP/ALB ratio) [AUC 0.943 (95% confidence interval (CI) = 0.911-0.976, p <  0.001)] and operation time [AUC 0.747 (95% CI = 0.679-0.815, p <  0.001)] had the greatest discrimination on AL prediction. Multivariate analysis demonstrated that CRP/ALB ratio and operation time were two independent risk factors for AL, and CRP/ALB ratio (OR = 102.909, p <  0.001) had an advantage over operation time (OR = 9.363, p = 0.020; Table 3).

Conclusion: Operation time and postoperative CRP/ALB ratio were two independent predictive indexes for AL. Postoperative CRP/ALB ratio greater than 3.00 indicated a high risk of AL. For patients with abnormal postoperative CRP/ALB ratio, early non-operative treatment or surgical intervention are needed to reduce the serious sequelae of AL.
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http://dx.doi.org/10.1186/s13019-021-01515-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130324PMC
May 2021

Low-dose IL-2 therapy limits the reduction in absolute numbers of circulating regulatory T cells in rheumatoid arthritis.

Ther Adv Musculoskelet Dis 2021 28;13:1759720X211011370. Epub 2021 Apr 28.

Department of Rheumatology, the Second Hospital of Shanxi Medical University.

Background: Circulating regulatory T cells (Tregs) are responsible for mediating immune tolerance and maintaining immunological homeostasis. Decreases in Tregs may be involved in the onset of rheumatoid arthritis (RA). Low-dose interleukin-2 (IL-2) has been considered for the treatment of inflammatory diseases mediated by T cells. This study focused on the status of circulating CD4T subsets and the clinical feasibility of IL-2 therapies in patients with RA.

Methods: The subjects included 888 patients with RA and 100 healthy controls (HCs); 233 RA patients received IL-2 treatment with 0.5 million international units (MIU)/day from days 1 through 5. The demographic features, disease activity, and levels of CD4+T cells measured by modified flow cytometry were collected in all RA patients before and after treatment.

Results: RA patients had lower absolute Treg counts (but not Th17) compared with HCs, which was associated with disease activity; previously treated RA patients had the fewest circulating Tregs (0.05). Patients treated with low-dose IL-2 had a three-fold increase in absolute anti-inflammatory Treg counts, as well as a two-fold increase in the other CD4T subsets. Moreover, post-treatment levels of markers of disease activity in RA patients treated with IL-2 were significantly lower than the baseline values (0.001), with no apparent side effects.

Conclusion: Decreased absolute counts of circulating CD4T lymphocyte subsets were observed in patients with RA. Circulating Tregs, which mediate immune tolerance, may be involved in the pathogenesis and progression of RA; however, this was ameliorated by low-dose IL-2, without obvious side effects.

Plain Language Summary: • Circulating Tregs may be involved in the pathogenesis and progression of RA.• The absolute count of Tregs was significantly correlated with disease activity measures.• Low-dose IL-2 was able to effectively expade Tregs and help for RA patients' symptoms remission without evaluated side effects.
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http://dx.doi.org/10.1177/1759720X211011370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107675PMC
April 2021

Total Flavonoids of Rhizoma Drynariae Restore the MMP/TIMP Balance in Models of Osteoarthritis by Inhibiting the Activation of the NF- and PI3K/AKT Pathways.

Evid Based Complement Alternat Med 2021 19;2021:6634837. Epub 2021 Apr 19.

Department of TCM Rheumatology, China-Japan Friendship Hospital, Beijing 100029, China.

Total flavonoids of (TFRD) have been shown to have beneficial effects on osteoarthritis (OA) clinically, but the mechanisms have not been elucidated. In this study, we investigated the effect of TFRD on articular cartilage in an OA rat model established by the Hulth method and in SW1353 chondrocytes induced by the proinflammatory factor interleukin-1 (IL-1). The results showed that TFRD could alleviate the pathological changes in knee cartilage in OA model rats. In vivo, the qPCR analysis indicated that the mRNA levels of matrix metalloproteinases, MMP-1, MMP-3, and MMP-13, were decreased, while tissue inhibitor of matrix metalloproteinases- (TIMP-) 4 was increased in cartilage, and these changes could be partially prevented by TFRD. In vitro experiments showed that IL-1 could significantly increase the expression of MMP-1, MMP-3, and MMP-13 and decrease the expression of TIMP-4 in SW1353 cells at the mRNA and protein levels. TFRD could increase the expression of MMP-3 and MMP-13 and decrease the expression of TIMP-4. Transfection of siRNA and addition of pathway inhibitors were used to clarify that inhibition of NF- and PI3K/AKT pathway decreased MMP-1, MMP-3, and MMP-13 and increased TIMP-4 expression. We also found that in IL-1-induced SW1353 cells, TFRD pretreatment had a modest inhibitory effect on p-AKT (Ser473) and reversed the increase of nuclear factor kappa-B (NF-) p65 in nuclear fraction and the decrease of inhibitor of NF-()- in the cytosolic fraction. Further immunofluorescence confirmed that TFRD can inhibit IL-1-induced NF- p65 translocation to the nucleus to some extent. In conclusion, TFRD showed chondroprotective effects by restoring the MMP/TIMP balance in OA models by suppressing the activation of the NF- and PI3K/AKT pathways.
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http://dx.doi.org/10.1155/2021/6634837DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081598PMC
April 2021