Publications by authors named "Jing Luo"

1,139 Publications

  • Page 1 of 1

Ceria nanoparticles prophylactic used for renal ischemia-reperfusion injury treatment by attenuating oxidative stress and inflammatory response.

Biomaterials 2022 Jul 18;287:121686. Epub 2022 Jul 18.

Institute of Immunology, PLA, Third Military Medical University (Army Medical University), Chongqing, 400038, China. Electronic address:

Renal ischemia-reperfusion (IR) injury (RIRI) is the leading cause of acute kidney injury (AKI), a common disease with high morbidity and mortality. However, due to the lack of effective diagnostic and therapeutic tools, patients have to resort to conservative treatment. To address this issue, we have developed a novel prophylactic strategy that involves the pre-treatment use of ceria nanoparticles (CNPs) before surgery. Based on our careful study of the three different sizes of CNPs that we synthesized, 46 nm (NP46), 81 nm (NP81), and 118 nm (NP118), we have found that NP118 can be used as effective prophylactic agents against RIRI and subsequent renal fibrosis. In our experiments, the CNPs exhibited excellent antioxidant and anti-inflammatory activities in vitro and effectively protected the kidney against RIRI and renal fibrosis in vivo, as proved by the decreases in renal lesions, serum creatinine, blood urea nitrogen, apoptotic cell, KIM-1 expression, and fibrotic area in CNPs treated samples relative to RIRI group. Mechanistically, not only did the CNPs reduce oxidative stress by regulating the Nrf2 pathway, but they also attenuated RIRI induced inflammatory response by decreasing macrophage infiltration and polarization to M1 phenotype, and reducing pro-inflammatory cytokine and chemokine production. In vitro results further confirmed that CNPs pre-treatment not only dramatically decreased intracellular ROS production in renal tubular epithelial cells and vascular endothelial cells, but also effectively attenuated lipopolysaccharide-induced inflammation in RAW264.7 cells. In addition, we found that one fourth of the NP118 persisted for more than 21 days in IR kidneys, and that out of the three sizes of CNPs, NP118 achieved the best results in all our experiments. Our study provides new insights into the usage and majorization of CNPs as a potential therapy to treat or prevent RIRI and renal fibrosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biomaterials.2022.121686DOI Listing
July 2022

Inhibition of c-MYC-miRNA 19 Pathway Sensitized CML K562 Cells to Etoposide via NHE1 Upregulation.

Oxid Med Cell Longev 2022 23;2022:9306614. Epub 2022 Jul 23.

Department of Immunology, Department of Biotherapy, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.

As a previously discovered target of DNA damage, Na/H exchanger 1 (NHE1) plays a role in regulation of intracellular pH (pH) through the extrusion of intracellular proton (H) in exchange for extracellular sodium (Na). Its abnormal expression and dysfunction have been reported in solid tumor and hematopoietic malignancies. Here, we reported that suppression of NHE1 in BCR-ABL hematopoietic malignancies' K562 cells treated with Etoposide was manipulated by miR-19 and c-MYC. Inhibition of miR-19 or c-MYC enhanced the expression of NHE1 and sensitized K562 cells to Etoposide . The nude mouse transplantation model was also performed to confirm the enhanced sensitivity of K562 cells to Etoposide by inhibiting the miR-19 or c-MYC pathway. TCGA analysis conferred a negative correlation between miR-19 level and leukemia patients' survival. Thus, our results provided a potential management by which the c-MYC-miRNA 19 pathway might have a crucial impact on sensitizing K562 cells to Etoposide in the therapeutic approaches.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/9306614DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338868PMC
August 2022

Safety and Efficacy of Programmed Cell Death 1 and Programmed Death Ligand-1 Inhibitors in the Treatment of Cancer: An Overview of Systematic Reviews.

Front Immunol 2022 13;13:953761. Epub 2022 Jul 13.

Department of Pharmacy, Sichuan Cancer Hospital and Institute, Sichuan Cancer Centre, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Background: An influx of systematic reviews (SRs) of programmed cell death 1 (PD-1) and programmed death ligand-1 (PD-L1) checkpoint inhibitors in cancer treatment with or without meta-analysis and with different methodological quality and inconsistent results have been published, confusing clinical decision making. The aim of this study was to comprehensively evaluate and summarize the current evidence of PD-(L)1 inhibitors in the treatment of cancer.

Methods: A comprehensive search of SRs, which included meta-analyses of PD-(L)1 inhibitors on cancer, was performed on eight databases with a cutoff date of 1 January 2022. Two authors independently identified SRs, extracted data, assessed the report quality according to the guidance of the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement, evaluated the methodological quality by the Assessment of Multiple Systematic Reviews 2 (AMSTAR 2), and appraised the quality of evidence by the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE).

Results: A total of 172 SRs with meta-analysis met the inclusion criteria. The report quality of included SRs was quite good, with 128 (74.42%) SRs of high quality and 44 (25.58%) of moderate quality. The methodological quality was alarming, as only one (0.58%) SR had high quality, five (2.91%) SRs had low quality, and the other 166 (96.51%) SRs had critically low quality. For GRADE, 38 (3.77%) outcomes had high-quality evidence, 288 (28.57%) moderate, 545 (54.07%) low, and 137 (13.59%) critically low-quality evidence. Current evidence indicated that treatment with PD-(L)1 inhibitors were significantly effective in non-small cell lung cancer, small cell lung cancer, hepatocellular carcinoma, malignant melanoma, renal cell carcinoma, and urothelial carcinoma, breast cancer, and head and neck squamous cell carcinoma with PD-L1 expression level≥1%, whereas the evidence in gastroesophageal and colorectal tumors is still controversial. Monotherapy with PD-(L)1 inhibitors was associated with a lower frequency of any grade and high-grade adverse events (AEs). The incidence of any grade and high-grade AEs caused by PD-(L)1 inhibitors in combination with other therapies was no lower than the controls. However, PD-(L)1 inhibitors were associated with a higher frequency of any grade and high-grade immune-related AEs.

Conclusions: PD-(L)1 inhibitors appeared to be effective and safe for cancer treatment, except for gastrointestinal tumors; however, the quality of the evidence is not convincing. Future studies should improve methodological quality and focus on the sequential trial analysis of subgroups and safety.

Systematic Review Registration: http://www.crd.york.ac.uk/prospero, identifier CRD42020194260.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.953761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326177PMC
August 2022

The validity and reliability of quality of life questionnaires in patients with ankylosing spondylitis and non-radiographic axial spondyloarthritis: a systematic review and meta-analysis.

Health Qual Life Outcomes 2022 Jul 30;20(1):116. Epub 2022 Jul 30.

Traditional Chinese Medicine Department of Rheumatism, China-Japan Friendship Hospital, Beijing, 100029, China.

Background: Patients who suffered from ankylosing spondylitis (AS) or non-radiographic axial spondyloarthritis (nr-axSpA) often have poor quality of life (QoL) and there has been a substantial increase in research on acceptable questionnaires for assessment of QoL. This systematic review aims at examining the validity and reliability of QoL questionnaires in patients with AS/nr-axSpA.

Methods: Randomized controlled trials (RCTs), cohort trials, and cross-sectional trails were retrieved by searching seven databases. Primary outcomes included test-retest reliability and construct validity. Secondary outcomes included internal consistency, structural validity, responsiveness and so on. Data extraction and analyses were conducted according to the Cochrane standards. The Agency for Healthcare Research and Quality (AHRQ) checklists was used to assess the risk of bias for each included study. We used the Consensus-based Standards for the Selection of Health Status Measurement Instruments (COSMIN) to assess the methodological quality and measurement property of included instruments. The quality of evidence on pre-specified outcomes were assessed by the Grades of Recommendations, Development and Evaluation (GRADE) approach.

Results: 22 publications containing 10 self-rating instruments were included in this study. Most studies were cross-sectional in design and a total of 3,085 participants were enrolled. 19 studies had moderate to high test-retest reliability. Cronbach's alpha (α) Coefficients were generally high (0.79-0.97) for overall scales. The ankylosing spondylitis quality of life (ASQOL) and evaluation of ankylosing spondylitis quality of life (EASi-QoL) questionnaires showed the strongest measurement properties in high-quality studies. The correlation coefficient for test-retest reliability of the ASQOL questionnaire was 0.85 (95% CI 0.80 to 0.89). The pooled Cronbach's α coefficients of the ASQOL questionnaire and the EASi-QoL questionnaire were high. Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) were considered as two validity criteria. For the ASQOL and EASi-QoL questionnaire, pooled convergent validity associations with BASDAI and BASFI were low to strong (0.24-0.81).

Conclusions: This study indicated acceptable reliability and stability of included QoL questionnaires. The ASQOL and the EASi-QoL questionnaires are validated and reliable disease-specific questionnaires for the assessment of QoL in patients with AS/nr-axSpA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12955-022-02026-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338652PMC
July 2022

Neuroimaging Assessment of Pain.

Neurotherapeutics 2022 Jul 28. Epub 2022 Jul 28.

Department of Sport Rehabilitation, Shanghai University of Sport, Shanghai, China.

Pain is an unpleasant sensory and emotional experience. Understanding the neural mechanisms of acute and chronic pain and the brain changes affecting pain factors is important for finding pain treatment methods. The emergence and progress of non-invasive neuroimaging technology can help us better understand pain at the neural level. Recent developments in identifying brain-based biomarkers of pain through advances in advanced imaging can provide some foundations for predicting and detecting pain. For example, a neurologic pain signature (involving brain regions that receive nociceptive afferents) and a stimulus intensity-independent pain signature (involving brain regions that do not show increased activity in proportion to noxious stimulus intensity) were developed based on multivariate modeling to identify processes related to the pain experience. However, an accurate and comprehensive review of common neuroimaging techniques for evaluating pain is lacking. This paper reviews the mechanism, clinical application, reliability, strengths, and limitations of common neuroimaging techniques for assessing pain to promote our further understanding of pain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s13311-022-01274-zDOI Listing
July 2022

Blood transcriptome analysis revealing aging gene expression profiles in red panda.

PeerJ 2022 22;10:e13743. Epub 2022 Jul 22.

Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, China.

The red panda is an endangered forest species distributed on the edge of the Qinghai Tibet Plateau. The species has been conserved in ex-situ in many countries and its survival is threatened by many diseases. Its immune system is vulnerable to age-associated alterations, which accumulate and result in a progressive deterioration that leads to an increased incidence of diseases. We identified 2,219 differentially expressed genes (DEGs) between geriatric (11-16 years) and adult individuals (4-8 years), and 1690 DEGs between adults and juveniles (1 year). The gene expression and functional annotation results showed that the innate immunity of red pandas increases significantly in geriatric individuals, whereas its change remains unclear when comparing adults and juveniles. We found that the adaptive immunity of red pandas first increased and then decreased with age. We identified CXCR3, BLNK, and CCR4 as the hub genes in the age-related protein-protein interaction network, which showed their central role in age-related immune changes. Many DNA repair genes were down-regulated in geriatric red pandas, suggesting that the DNA repair ability of the blood tissue in geriatric red pandas is significantly reduced. The significantly up-regulated TLR5 in geriatric individuals also suggests the possibility of enhancing the vaccination immune response by incorporating flagellin, which could be used to address decreased vaccine responses caused by age-related declines in immune system function. This work provides an insight into gene expression changes associated with aging and paves the way for effective disease prevention and treatment strategies for red pandas in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.13743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310792PMC
July 2022

Different Responses of Invasive Weed and to Plant Growth Regulators.

Life (Basel) 2022 Jul 17;12(7). Epub 2022 Jul 17.

School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.

Invasive plants cause a global loss of biodiversity, pose a major threat to the environment and economy, and also significantly affect agricultural production and food security. Plant growth regulators (PGRs) are widely used in agricultural production and might also affect invasive weeds distributed around crops in various ways. At present, there are few studies concerning whether there are significant effects of PGRs on invasive weeds. In this study, two widely used PGRs in paddy fields, gibberellic acid (GA) and paclobutrazol (PAC), were applied on and a noxious weed , which is frequently distributed in paddy fields. The purpose of this study was to investigate if there are different responses of rice and weeds to these two plant regulators and the significant effects of PGRs on invasive weeds. The results showed that GA significantly promotes the total biomass of by 52.00%, but does not significantly affect that of . GA significantly increases the growth of aboveground and belowground , but not that of . On the other hand, PAC extremely inhibited the aboveground and belowground biomass of by more than 90%, but did not significantly inhibit the belowground biomass of . PAC also enhanced the leaf nitrogen content and chlorophyll content of , but not the traits of . Therefore, the effects of PGRs are significantly different between rice and the invasive weed. The potential promotion effects of PGRs on weeds that are frequently distributed in farmland warrant sufficient attention. This is probably one of the important reasons why invasive weeds can successfully invade the agricultural ecosystem with large human disturbance. This study might sound an alarm for weed control in paddy fields.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/life12071069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318923PMC
July 2022

Transcranial Direct Current Stimulation Over the Right Temporal Parietal Junction Facilitates Spontaneous Micro-Expression Recognition.

Front Hum Neurosci 2022 8;16:933831. Epub 2022 Jul 8.

State Key Laboratory of Cognitive Neuroscience and Learning and IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.

Micro-expressions are fleeting and subtle emotional expressions. As they are spontaneous and uncontrollable by one's mind, micro-expressions are considered an indicator of genuine emotions. Their accurate recognition and interpretation promote interpersonal interaction and social communication. Therefore, enhancing the ability to recognize micro-expressions has captured much attention. In the current study, we investigated the effects of training on micro-expression recognition with a Chinese version of the Micro-Expression Training Tool (METT). Our goal was to confirm whether the recognition accuracy of spontaneous micro-expressions could be improved through training and brain stimulation. Since the right temporal parietal junction (rTPJ) has been shown to be involved in the explicit process of facial emotion recognition, we hypothesized that the rTPJ would play a role in facilitating the recognition of micro-expressions. The results showed that anodal transcranial direct-current stimulation (tDCS) of the rTPJ indeed improved the recognition of spontaneous micro-expressions, especially for those associated with fear. The improved accuracy of recognizing fear spontaneous micro-expressions was positively correlated with personal distress in the anodal group but not in the sham group. Our study supports that the combined use of tDCS and METT can be a viable way to train and enhance micro-expression recognition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2022.933831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305610PMC
July 2022

Network Pharmacology-Based Strategy to Investigate the Mechanisms of in Treating Osteoarthritis.

Evid Based Complement Alternat Med 2022 14;2022:1826299. Epub 2022 Jul 14.

Department of TCM Rheumatology, China-Japan Friendship Hospital, Beijing 100029, China.

is a representative tonifying kidney drug and is widely used for osteoarthritis (OA) in traditional Chinese medicine. However, its regulatory mechanisms in treating OA remain to be sufficiently investigated. The main chemical components of were screened through the TCMID database and the corresponding targets were acquired through SwissTargetPrediction. The OA-related targets were obtained from the OMIM, Genecards, Genebank, TTD, and DisGeNET databases. The prediction of key targets and pathways of in the treatment of OA was achieved by constructing a compounds-targets network and performing KEGG enrichment analysis. The OA model rats were established by the Hulth method and used to explore the protective effect of via cartilage pathological assessment. In vitro models of OA were built by the proinflammatory factor interleukin-1 (IL-1) induced SW1353 cells and used to validate the mechanisms predicted by network pharmacology. Network pharmacology results suggested that the therapeutic effects of were closely related to matrix metalloproteinase (MMP)-1, 3, 13 and inflammation-related gene COX2, which are regulated by the NFB pathway. In vivo experiments revealed that could effectively restrain cartilage from degeneration and inhibit the mRNA expression of MMP-1, MMP-3, MMP-13, and COX2 in cartilage. In vitro experiments indicated that water extract (CBWE) could significantly inhibit the expression of MMP-1, MMP-3, MMP-13, and PGE in IL-1-induced SW1353 cells and markedly prevent the translocation of NFB p65 from the cytoplasm to the nuclei and decrease its phosphorylation level. After small-interfering RNA (siRNA) was used to suppress the synthesis of NFB p65 to block NFB signaling pathway, the ability of CBWE to inhibit MMP-1, MMP-3, MMP-13, and PGE was greatly reduced. has a chondroprotective effect on OA by inhibiting the response to inflammation and substrate degradation, and the related mechanism is associated with the inhibition of the NFB pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/1826299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303148PMC
July 2022

A Longitudinal Investigation of the Causal Relationship Between Wellbeing and Perceived Discrimination Among Migrant Children in China: The Mediating Role of Self-Esteem and the Moderating Role of School Type.

Front Psychiatry 2022 8;13:899888. Epub 2022 Jul 8.

Beijing Key Laboratory of Learning and Cognition, School of Psychology, Capital Normal University, Beijing, China.

Background: A large rural labor force has been attracted to urban areas with the acceleration of urbanization in China. This significant change in environment for migrant children from rural to urban may lead to psychological problems, such as decreased subjective wellbeing (WB) and increased perceived discrimination (PD). However, previous studies have focused on the influence of PD on WB by using a cross-sectional design, ignoring the causality and intrinsic mechanisms between WB and PD. The current study investigates the causal association and internal relations between migrant children's PD and WB.

Methods: A total of 466 (222 females, 47.64%) migrant children ( = 11.78, = 1.80) were recruited from Beijing in China. The participants filled in the questionnaire twice, with an interval of 1 year, including a basic information questionnaire, wellbeing index scale, perceived discrimination questionnaire, and self-esteem scale.

Results: Overall, cross-lagged regression analysis revealed that WB (T1) had a predictive effect on PD (T2) but that PD (T1) had no predictive effect on WB (T2). Mediation results indicated that self-esteem (SE) (T1) mediated the relation between WB (T1) and PD (T2). Moderated mediation results further proved that the link between WB (T1) on SE (T1) and the indirect effect between WB (T1) and PD (T2) were more robust for migrant children who attended public school than those in the migrant children's school.

Conclusion: These findings implied that a decrease in WB may increase the perception of subjective discrimination and that SE could be an intrinsic factor between migrant children's WB and PD, especially in public schools. Therefore, educators and parents should also pay attention to mental health problems to improve the wellbeing and self-esteem of migrant children.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyt.2022.899888DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305607PMC
July 2022

An Osimertinib-Perfluorocarbon Nanoemulsion with Excellent Targeted Therapeutic Efficacy in Non-small Cell Lung Cancer: Achieving Intratracheal and Intravenous Administration.

ACS Nano 2022 Jul 21. Epub 2022 Jul 21.

NHC and CAMS Key Laboratory of Molecular Probe and Targeted Theranostics, Molecular Imaging Research Center (MIRC), Harbin Medical University, 150028 Harbin, Heilongjiang, China.

Low accumulation of anticancer drugs in tumors and serious systemic toxicity remain the main challenges to the clinical efficiency of pharmaceuticals. Pulmonary delivery of nanoscale-based drug delivery systems offered a strategy to increase antitumor activity with minimal adverse exposure. Herein, we report an osimertinib-loaded perfluoro-15-crown-5-ether (AZD9291-PFCE) nanoemulsion, through intratracheal and intravenous delivery, synergizes with F magnetic resonance imaging (F MRI)-guided low-intensity focused ultrasound (LIFU) for lung cancer therapy. Pulmonary delivery of AZD9291-PFCE nanoemulsion in orthotopic lung carcinoma models achieves quick distribution of the nanoemulsion in lung tissues and tumors without short-term and long-term toxic effects. Furthermore, LIFU can trigger drug release from the AZD9291-PFCE nanoemulsion and specifically increases tumor vascular and tumor tissue permeability. F MRI was applied to quantify nanoemulsion accumulation in tumors in real time after LIFU irradiation. We validate the treatment effect of AZD9291-PFCE nanoemulsion in resected human lung cancer tissues, proving the translational potential to enhance clinical outcomes of lung cancer therapy. Thus, this work presents a promising pulmonary nanoemulsion delivery system of osimertinib (AZD9291) for targeted therapy of lung cancer without severe side effects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsnano.2c04159DOI Listing
July 2022

Tumor budding of cervical squamous cell carcinoma: epithelial-mesenchymal transition-like cancer stem cells?

PeerJ 2022 15;10:e13745. Epub 2022 Jul 15.

Department of Pathology, Meizhou People's Hospital, Meizhou, Guangdong, China.

Recent evidence indicates that cancer stem cells (CSCs) are the origin of cancers. Scientists have identified CSCs in various tumors and have suggested the existence of a variety of states of CSCs. The existence of epithelial-mesenchymal transition (EMT)-like CSCs has been confirmed , but they have not been identified . Tumor budding was defined as single cell or clusters of ≤ 5 cells at the invasive front of cancers. Such tumor budding is hypothesized to be closely related to EMT and linked to CSCs, especially to those migrating at the invasive front. Therefore, tumor budding has been proposed to represent EMT-like stem cells. However, this hypothesis has not yet been proven. Thus, we studied the expression of EMT markers, certain CSC markers of tumor budding, and the tumor center of cervical squamous cell carcinoma (CxSCC). We performed tissue chip analyses of 95 primary CxSCCs from patients. Expression of EMT and CSC markers (E-cadherin, -catenin, vimentin, Ki67, CD44, SOX2 , and ALDH1A1) in a set of tumor samples on tissue chips (87 cases of tumor budding/the main tumor body) were evaluated by immunohistochemistry. We found that the cell-membranous expression of -catenin was stronger in the main tumor body than in tumor buds. Compared with the main tumor body, tumor buds had reduced proliferative activity as measured by Ki67. Moreover, vimentin expression was high and E-cadherin expression was low in tumor buds. Expression of EMT-related markers suggested that tumor buds were correlated with EMT. We noted that CxSCC tumor buds had a CD44/SOX2/ALDH1A1 staining pattern, indicating that tumor buds of CxSCC present CSC-like immunophenotypic features. Taken together, our data indicate that tumor buds in CxSCC may represent EMT-like CSCs
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.13745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291004PMC
July 2022

Manual acupuncture as prophylaxis for migraine without aura: study protocol for a multi-center, randomized, single-blinded trial.

Trials 2022 Jul 19;23(1):574. Epub 2022 Jul 19.

Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, No.37 Shier Qiao Road, Chengdu, 610075, Sichuan, China.

Background: Migraine is a highly prevalent neurological disorder. It is the third most prevalent disorder and the seventh highest cause of disability worldwide. Acupuncture may be a viable prophylactic treatment option for frequent or uncontrolled migraine. Clinical studies comparing acupuncture and placebo acupuncture have not reached a consistent conclusion in confirming whether acupuncture is effective in migraine prophylaxis. The effect of acupuncture mainly depends on acupoints and needles operation. We found that the design of the placebo acupuncture in previous studies included shallow needling at sham acupoints, non-penetrating needling at sham acupoints, and needling at inactive acupuncture points to achieve the inert effect of control group, but the non-penetrating needling at true acupoints was ignored. This randomized controlled trial aims to use true acupoints for non-penetrating acupuncture as control to evaluate the efficacy of manual acupuncture for the prophylaxis of migraine without aura (MWoA).

Methods/design: This is a single-blinded, randomized, controlled, prospective, multi-center trial with two parallel treatment groups. A total of 198 eligible patients with MWoA will be randomly divided into two groups (1:1 allocation ratio). The intervention group will receive manual acupuncture and the control group will receive placebo acupuncture (non-penetrating). Patients will receive three acupuncture treatment sessions per week for 4 consecutive weeks. All patients will then receive a 12-week follow-up.

Discussion: In this study, we are evaluating the efficacy and safety of manual acupuncture in the prophylaxis of MWoA. The placebo control is using non-penetrating needling verum acupoints. It is essential to determine an appropriate control method to ensure the methodological quality of a randomized controlled trial.

Trial Registration: The trial has been registered in the Chinese Clinical Trial Registry (approval no. ChiCTR2000032308 ) in April 2020.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13063-022-06510-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295267PMC
July 2022

A case report of the metagenomics next-generation sequencing for early detection of central nervous system mucormycosis with successful rescue in patient with recurrent chronic lymphocytic leukemia.

Ann Transl Med 2022 Jun;10(12):722

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Central nervous system (CNS) mucormycosis is insidious and difficult to diagnose. It progresses rapidly and causes high mortality. Rare cases have been reported during ibrutinib use, which have poor prognosis. Through this case, we share the experience of successful diagnosis and treatment. We also emphasize the importance of focusing on high-risk groups, early diagnosis and prompt management.

Case Description: In this case, a 52-year-old patient was diagnosed with chronic lymphocytic leukemia (CLL) for more than 5 years. He was in remission after rituximab plus fludarabine and cyclophosphamide (RFC) regimen, and relapsed in the fourth year. During the ibrutinib monotherapy, the patient presented with sudden headache. Cranial imaging examination revealed a definite right occipitoparietal lobe mass with extensive edema. A rapid diagnosis of mucormycosis infection was made using metagenomic next-generation sequencing (mNGS). The patient at that time didn't have neutropenia, but he had hypogammaglobulinemia. The infection was treated with amphotericin B cholesteryl sulfate complex, posaconazole, and interventional surgery, and the treatment was successful. At the same time, we considered the control of disease progression in this relapsed patient with, as well as to the drug interaction with posaconazole. We chose the next generation Bruton's tyrosine kinase (BTK) inhibitor zanubrutinib as the treatment, whose safety has been identified. As of the submission date, the patient has been followed up for nearly 1 year, and his disease is stable.

Conclusions: When new clinical problems arise in recurrent CLL patients, it is important to identify multiple factors, especially the insidious fungal infections. In particular, the immunocompromised patients should be concerned. CNS mucormycosis is extremely deadly, the early diagnosis will improve the prognosis. In clinical practice, the gold standard diagnosis of mucormycosis is difficult to obtain through pathology. In this case, mNGS was applied to quickly diagnose mucormycosis, enabling earlier treatment and ameliorating the prognosis. Thus, it will help us to early detect this group of people who may be potentially infected. Current guidelines do not recommend the prophylactic use of antifungal agents in treated CLL patients. However, in patients with prior severe infection or hypogammaglobulinemia, intravenous immunoglobulin is recommended to reduce the associated infection rate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-22-2533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279813PMC
June 2022

Expression of serum miR-126 in patients with intracranial aneurysm and its relationship with postoperative cerebral vasospasm.

Am J Transl Res 2022 15;14(6):4372-4379. Epub 2022 Jun 15.

Department of Pharmacology, Basic Medicine College, Anhui Medical University Hefei 230032, Anhui, PR China.

Objective: To examine the expression of serum miR-126 in patients with intracranial aneurysm and to explore its relationship with postoperative cerebral vasospasm.

Methods: In this retrospective study, 85 patients with intracranial aneurysms diagnosed and treated in our hospital were enrolled into the research group (RG), and 83 healthy volunteers who came to our hospital for physical examination were selected as the control group (CG). The serum miR-126 expression in both groups was examined by RT-PCR, and the relationship between the diagnostic value of miR-126 for intracranial aneurysm and postoperative cerebral vasospasm was analyzed. The serum inflammatory related factors in the patients were tested, and their correlation with miR-126 was assessed. The risk factors of postoperative cerebral vasospasm were evaluated by multiple factors.

Results: The serum miR-126 expression in patients with intracranial aneurysm was obviously lower than that of participants in CG (P<0.05), and the AUC of miR-126 in diagnosing intracranial aneurysm was 0.945, which was of high diagnostic value. Serum inflammatory factors TNF-α and IL-6 were highly expressed in the serum of patients with intracranial aneurysm, which were positively correlated with the miR-126 level (P<0.05). After operation, the serum miR-126 level in patients with cerebral vasospasm was obviously higher than that of those without cerebral vasospasm, and the AUC of miR-126 for predicting cerebral vasospasm after operation was 0.859. Logistic regression analysis revealed that preoperative bleeding frequency, history of hypertension, Hunt-Hess grade and high expression of miR-126 were independent risk factors for cerebral vasospasm after operation in patients with intracranial aneurysm.

Conclusion: miR-126 is highly expressed in the serum of patients with intracranial aneurysm, so it may be used as a potential biomarker for the diagnosis of patients with intracranial aneurysm and the prediction of cerebral vasospasm after operation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274600PMC
June 2022

Recombinant High-Density Lipoprotein Boosts the Therapeutic Efficacy of Mild Hypothermia in Traumatic Brain Injury.

ACS Appl Mater Interfaces 2022 Jul 14. Epub 2022 Jul 14.

Department of Pharmacology and Chemical Biology, Shanghai Universities Collaborative Innovation Center for Translational Medicine, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China.

Traumatic brain injury (TBI) leads to neuropsychiatric symptoms and increased risk of neurodegenerative disorders. Mild hypothermia is commonly used in patients suffering from severe TBI. However, its effect for long-term protection is limited, mostly because of its insufficient anti-inflammatory and neuroprotective efficacy and restricted treatment duration. Recombinant high-density lipoprotein (rHDL), which possesses anti-inflammatory and antioxidant activity and blood-brain barrier (BBB) permeability, was expected to potentially strengthen the therapeutic effect of mild hypothermia in TBI treatment. To test this hypothesis and optimize the regimen for combination therapy, the efficacy of mild hypothermia plus concurrent or sequential rHDL on oxidative stress, inflammatory reaction, and cell survival in the damaged brain cells was evaluated. It was found that the effect of combining mild hypothermia with concurrent rHDL was modest, as mild hypothermia inhibited the cellular uptake and lesion-site-targeting delivery of rHDL. In contrast, the combination of mild hypothermia with sequential rHDL more powerfully improved the anti-inflammatory and antioxidant activities, promoted nerve cell survival and BBB restoration, and ameliorated neurologic changes, which thus remarkably restored the spatial learning and memory ability of TBI mice. Collectively, these findings suggest that rHDL may serve as a novel nanomedicine for adjunctive therapy of TBI and highlight the importance of timing of combination therapy for optimal treatment outcome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c02940DOI Listing
July 2022

Effect of High Myopia on Delayed Absorption of Subretinal Fluid after Scleral Buckling Surgery.

J Clin Med 2022 Jul 5;11(13). Epub 2022 Jul 5.

Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Changsha 410011, China.

This study compared the absorption of subretinal fluid (SRF) in patients with rhegmatogenous retinal detachment (RRD) with and without high myopia after scleral buckling (SB) and investigated the effect of high myopia on SRF absorption. This retrospective study included patients with primary macula-off RRD grouped according to myopia and age. The optical coherence tomography (OCT) and OCT angiography indicators included subretinal fluid height (SRFH), subfoveal choroidal thickness (SFCT), and choroidal capillary blood flow density (CCFD) measured regularly. The presence of SRF 3 months after surgery was defined as delayed absorption. Overall, 90 eyes of 89 patients were enrolled, and 46 eyes (51.11%) had high myopia. In 43 eyes (47.78%), SRF absorption was delayed. There was no significant difference in SRF absorption after SB between the high and non-high myopia groups; younger patients (<35 years) had a higher probability of delayed absorption ( < 0.05). The SFCT in high myopia was significantly thinner than that in the non-high myopia group ( < 0.05); SFCT and SRFH were positively correlated (r = 0.275, = 0.002), and there was a significant difference between the average CCFD with and without SRF ( < 0.05). High myopia had no significant effect on SRF absorption after SB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jcm11133906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267211PMC
July 2022

The Stressful Personality: A Meta-Analytical Review of the Relation Between Personality and Stress.

Pers Soc Psychol Rev 2022 Jul 8:10888683221104002. Epub 2022 Jul 8.

University of Illinois at Urbana-Champaign, USA.

The current study presented the first meta-analytic review on the associations between the Big Five personality traits and stress measured under different conceptualizations (stressor exposure, psychological and physiological stress responses) using a total of 1,575 effect sizes drawn from 298 samples. Overall, neuroticism was found to be positively related to stress, whereas extraversion, agreeableness, conscientiousness, and openness were negatively linked to stress. When stress assessed under different conceptualizations was tested, only neuroticism, agreeableness, and conscientiousness were related to stressor exposure. All of the Big Five personality traits were significantly associated with psychological stress perception, whereas the five personality traits showed weak to null associations with physiological stress response. Further moderation analyses suggested that the associations between personality traits and stress under different conceptualizations were also contingent upon different characteristics of stress, sample, study design, and measures. The results supported the important role of personality traits in individual differences in stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/10888683221104002DOI Listing
July 2022

Potential brain recovery of frontostriatal circuits in heroin users after prolonged abstinence: A preliminary study.

J Psychiatr Res 2022 Aug 28;152:326-334. Epub 2022 Jun 28.

Center for Brain Imaging, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, 710126, China; Engineering Research Center of Molecular and Neuroimaging, Ministry of Education, Xi'an, Shaanxi, 710071, China; International Joint Research Center for Advanced Medical Imaging and Intelligent Diagnosis and Treatment & Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, 710126, China; Information Processing Laboratory, School of Information Engineering, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia, 014010, China. Electronic address:

Neuroscientists have devoted efforts to explore potential brain recovery after prolonged abstinence in heroin users (HU). However, not much is known about whether frontostriatal circuits can recover after prolonged abstinence in HU. An eight-month longitudinal study was carried out for HU. Two MRI scans were obtained at baseline (HU1) and 8-month follow-up (HU2). The functional and structural connectivities of dorsal and ventral frontostriatal pathways were measured by resting-state functional connectivity (RSFC) and diffusion tensor imaging (DTI). Correlation analyses were employed to reveal the associations between neuroimaging and behavioral changes. Results suggested that relative to healthy controls (HCs), HU1 showed lower fractional anisotropy (FA) in the right dorsolateral prefrontal cortex (DLPFC)-to-caudate tracts and medial orbitofrontal cortex (mOFC)-to-nucleus accumbens (NAc) tracts as well as decreased RSFC in the left mOFC-NAc circuits. Longitudinal results revealed reduced craving and enhanced cognitive control in HU2 compared with HU1. After prolonged abstinence, HU2 showed increased FA values in the right DLPFC-caudate and mOFC-NAc tracts as well as increased RSFC strength in the bilateral mOFC-NAc circuits compared with HU1. In addition, changes in RSFC and FA values in the right mOFC-NAc circuit were negatively correlated with craving score changes. Similarly, negative correlations were also found between changes of RSFC in the bilateral DLPFC-caudate circuits and TMT-A scores. We provided scientific evidence for brain recovery of the dorsal and ventral frontostriatal circuits in HU after prolonged abstinence, and these circuits may be potential neuroimaging biomarkers for cognition and craving changes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpsychires.2022.06.036DOI Listing
August 2022

Orange-emissive carbon quantum dots for ligand-directed Golgi apparatus-targeting and imaging.

Biomater Sci 2022 Jul 26;10(15):4345-4355. Epub 2022 Jul 26.

Ophthalmology Department, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongii Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China.

The Golgi apparatus is one of the most important organelles in cells. Targeting and monitoring the morphology and structure of the Golgi apparatus are crucial and challenging. Aimed at the cysteine (Cys) receptor on the surface of the Golgi apparatus, ligand-directed carbon quantum dots (CQDs) were synthesized for Golgi apparatus-targeting imaging. In order to reduce the interference of tissue self-fluorescence and enhance the tissue penetration depth, orange-emissive levorotatory CQDs (L-CQDs) with Golgi apparatus-targeting ability were synthesized using the strategy of inheriting Cys residues and the inherent conjugated electronic structure of neutral red. They exhibit excitation-dependent, fluorescence stability, rich surface Cys residues, excellent biocompatibility, and low toxicity. As a Golgi apparatus-targeting agent, L-CQDs can quickly enter cells for Golgi apparatus-targeting imaging, and can also penetrate through biological tissue for imaging . The surface Cys residues of CQDs actively target the Cys receptors on the surface of the Golgi apparatus to achieve Golgi apparatus-targeting imaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d2bm00429aDOI Listing
July 2022

Tracheal Lobular Capillary Hemangioma.

Am J Respir Crit Care Med 2022 Jul 1. Epub 2022 Jul 1.

Hunan Provincial People's Hospital (First Affiliated Hospital of Hunan Normal University), Changsha, China;

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1164/rccm.202202-0297IMDOI Listing
July 2022

One-Month Outcomes of Intravitreal Anti-VEGF vs. Dexamethasone Implant in the Treatment of Diabetic Macular Edema in Vitrectomized Eyes.

Front Med (Lausanne) 2022 14;9:895220. Epub 2022 Jun 14.

Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.

Objectives: To compare short-term effect of intravitreal ranibizumab with dexamethasone implant for diabetic macular edema (DME) in vitrectomized eyes.

Methods: Single-center, prospective, randomized study of vitrectomized eyes with DME. Study eyes were divided into two groups, receiving ranibizumab (IVV group, = 35 eyes) or dexamethasone implant (IVD group, = 35 eyes) respectively. Patients were evaluated at baseline, Week 1 and Month 1. The main outcome measures included best-corrected visual acuity (BCVA), central retinal thickness (CRT) and intraocular pressure (IOP).

Results: BCVA and CRT were similar in the two groups at baseline. At Week 1, the CRT improvement was significant in two groups ( = 0.041 in IVV group, = 0.030 in IVD group), but at Month 1, only IVD group had significant improvement in CRT ( < 0.001). And BCVA gains were significant at Week 1 ( = 0.029) and Month 1 ( = 0.001) in IVD group, whereas IVV group did not show significant BCVA gains ( = 0.056 at Week1, = 0.166 at Month 1). The changes of BCVA and CRT were significantly higher in IVD group than IVV group at Month1, but the changes were not significant at Week1.

Conclusions: Comparing to anti-VEGF therapy, DEX implant is more effect in improving BCVA and reducing CRT in vitrectomized eyes at 1 month, which indicated DEX implant is a better strategy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2022.895220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237330PMC
June 2022

Tophi on the Retina: An Intraocular Manifestation of Gout.

Ophthalmology 2022 Jun 25. Epub 2022 Jun 25.

Department of Ophthalmology, The Second Xiangya Hospital, Changsha, Hunan Province, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ophtha.2022.02.016DOI Listing
June 2022

Comparing Mouse and Human Tissue-Resident γδ T Cells.

Front Immunol 2022 8;13:891687. Epub 2022 Jun 8.

Institute of Reproductive Health, Center for Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Circulating immune cell compartments have been extensively studied for decades, but limited access to peripheral tissue and cell yield have hampered our understanding of tissue-based immunity, especially in γδ T cells. γδ T cells are a unique subset of T cells that are rare in secondary lymphoid organs, but enriched in many peripheral tissues including the skin, uterus, and other epithelial tissues. In addition to immune surveillance activities, recent reports have revealed exciting new roles for γδ T cells in homeostatic tissue physiology in mice and humans. It is therefore important to investigate to what extent the developmental rules described using mouse models transfer to human γδ T cells. Besides, it will be necessary to understand the differences in the development and biogenesis of human and mouse γδ T cells; to understand how γδ T cells are maintained in physiological and pathological circumstances within different tissues, as well as characterize the progenitors of different tissue-resident γδ T cells. Here, we summarize current knowledge of the γδ T phenotype in various tissues in mice and humans, describing the similarities and differences of tissue-resident γδ T cells in mice and humans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.891687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9215113PMC
June 2022

Comparative Efficacy and Safety of Poly (ADP-Ribose) Polymerase Inhibitors in Patients With Ovarian Cancer: A Systematic Review and Network Meta-Analysis.

Front Oncol 2022 8;12:815265. Epub 2022 Jun 8.

Sichuan Cancer Hospital & Institute, Sichuan Cancer Centre, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Objective: This study aims to compare the efficacy and safety of different poly (ADP-ribose) polymerase (PARP) inhibitors in patients with ovarian cancer through a network meta-analysis to support clinical treatment choices.

Methods: The Cochrane Library, PubMed, Embase, Science Citation Index, China National Knowledge Infrastructure (CNKI), Wanfang Data, Chongqing VIP (CQVIP), and Chinese BioMedical Literature Database (CBM) were searched with a cutoff date of 14 January 2021. ClinicalTrials.gov was also checked for supplementary data. Phase II or III randomized controlled trials that compared a PARP inhibitor with a placebo in patients with relapsed or newly diagnosed advanced ovarian cancer were included. The hazard ratios (HRs) for progression-free survival and overall survival and odds ratios (ORs) for grade 3 or higher adverse events were analyzed. The network meta-analysis was conducted in a Bayesian framework based on the Markov Chain Monte Carlo model in the R gemtc package (version 4.0.3).

Results: Eight eligible articles reporting six trials with a total of 2,801 patients were incorporated in this network meta-analysis. Three trials compared olaparib with placebo. Two trials compared niraparib with placebo. One trial compared rucaparib with placebo. The network meta-analysis failed to show significant differences in progression-free survival among the three PARP inhibitors: HR of 0.64, 95% confidence interval of 0.3 to 1.42 for olaparib versus niraparib, and olaparib versus rucaparib (0.86; 0.33 to 2.33). The comparison between niraparib and rucaparib also did not express a statistical difference (1.34; 0.47 to 3.72). Subgroup analysis bybreast cancer susceptibility gene (BRCA) status showed no obvious difference in progression-free survival among the three PARP inhibitors regardless of BRCA mutation status. Olaparib had fewer grade 3 or higher adverse events than niraparib (OR, 0.27; 95% confidence interval, 0.13 to 0.55) and rucaparib (0.34; 0.14 to 0.86). However, the analysis failed to show a significant difference between niraparib and rucaparib (1.27; 0.49 to 3.27).

Conclusion: Current evidence indicates that there is no significant difference observed in efficacy among olaparib, niraparib, and rucaparib. However, olaparib might have fewer grade 3 or higher adverse events.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2022.815265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213680PMC
June 2022

Corrigendum: Association of αENaC p. Ala663Thr Gene Polymorphism With Sudden Sensorineural Hearing Loss.

Front Genet 2022 9;13:931037. Epub 2022 Jun 9.

Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

[This corrects the article DOI: 10.3389/fgene.2021.659517.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2022.931037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219908PMC
June 2022

Cognitive control of invalid predominant ideas in insight-like problem solving.

Psychophysiology 2022 Jun 25:e14133. Epub 2022 Jun 25.

School of Psychology, Jiangxi Normal University, Nanchang, China.

Predominant ordinary ideas are insufficient for solving insight-like problems; they interfere with subordinate original ideas and can produce a mental impasse. However, how people monitor and control invalid predominant ideas remains largely unknown. In the current study, participants were asked to solve a sequence of several similar practice problems that had the same solution to strengthen a predominant idea; the participants were then presented with an insight-like test problem that could not be solved by the predominant idea. The results showed that if the test problem was similar to the practice problems in which the predominant idea could typically be applied, it elicited greater late sustained potential (LSP) over the whole brain but no conflict-related N2 or N400 components, which suggests that the participants did not experience cognitive conflict and continued to verify the predominant but currently invalid idea. When the test problem differed from the practice problems, the items that participants reported trying to solve elicited greater N2-N400 and LSP over the whole brain, which suggests that the participants experienced cognitive conflict and exerted more reactive control over the invalid predominant idea; in contrast, the items that participants reported thinking about how to solve did not evoke greater conflict-related N2-N400 components and evoked even lower LSP, which likely indicates an ineffective state. These findings demonstrate three kinds of cognitive control toward invalid predominant ideas in situations where they are typically and not typically applied and provide empirical evidence of a mental impasse in insight-like problem-solving behaviors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/psyp.14133DOI Listing
June 2022

Clinical significance of cyclin-dependent kinase inhibitor 2C expression in cancers: from small cell lung carcinoma to pan-cancers.

BMC Pulm Med 2022 Jun 24;22(1):246. Epub 2022 Jun 24.

Ward of Pulmonary and Critical Care Medicine, Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, 530021, Guangxi Zhuang Autonomous Region, People's Republic of China.

Background: Cyclin-dependent kinase inhibitor 2C (CDKN2C) was identified to participate in the occurrence and development of multiple cancers; however, its roles in small cell lung carcinoma (SCLC) remain unclear.

Methods: Differential expression analysis of CDKN2C between SCLC and non-SCLC were performed based on 937 samples from multiple centers. The prognosis effects of CDKN2C in patients with SCLC were detected using both Kaplan-Meier curves and log-rank tests. Using receiver-operating characteristic curves, whether CDKN2C expression made it feasible to distinguish SCLC was determined. The potential mechanisms of CDKN2C in SCLC were investigated by gene ontology terms and signaling pathways (Kyoto Encyclopedia of Genes and Genomes). Based on 10,080 samples, a pan-cancer analysis was also performed to determine the roles of CDKN2C in multiple cancers.

Results: For the first time, upregulated CDKN2C expression was detected in SCLC samples at both the mRNA and protein levels (p of Wilcoxon rank-sum test < 0.05; standardized mean difference = 2.86 [95% CI 2.20-3.52]). Transcription factor FOXA1 expression may positively regulate CDKN2C expression levels in SCLC. High CDKN2C expression levels were related to the poor prognosis of patients with SCLC (hazard ratio > 1, p < 0.05) and showed pronounced effects for distinguishing SCLC from non-SCLC (sensitivity, specificity, and area under the curve ≥ 0.95). CDKN2C expression may play a role in the development of SCLC by affecting the cell cycle. Furthermore, the first pan-cancer analysis revealed the differential expression of CDKN2C in 16 cancers (breast invasive carcinoma, etc.) and its independent prognostic significance in nine cancers (e.g., adrenocortical carcinoma). CDKN2C expression was related to the immune microenvironment, suggesting its potential usefulness as a prognostic marker in immunotherapy.

Conclusions: This study identified upregulated CDKN2C expression and its clinical significance in SCLC and other multiple cancers, suggesting its potential usefulness as a biomarker in treating and differentiating cancers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12890-022-02036-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233395PMC
June 2022

Overexpression of Increases Pyrethrin Content When Using a Genotype-Independent Transformation System in Pyrethrum ().

Plants (Basel) 2022 Jun 15;11(12). Epub 2022 Jun 15.

Key Laboratory for Biology of Horticultural Plants, Ministry of Education, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.

Pyrethrum () is one of the most important industrial crops for the extraction of pyrethrins, which are natural insecticidal compounds. Progress in pyrethrum molecular breeding with the objective of increasing pyrethrin content has been slow for lack of a suitable gene transfer system. Regeneration recalcitrance is a crucial barrier to establishing a genetic transformation system in pyrethrum. Therefore, in this study, an Agrobacterium-mediated transformation system in pyrethrum was developed using shoot apical meristems from germinated seedlings. Factors affecting transformation efficiency were optimized. Optimal conditions included explants at the "no true leaf" stage with a half apical meristem, an cell density of OD = 0.5, two days of cocultivation, and the incorporation of 1.5 mg L 6-BA and 30 mg L kanamycin into the selection medium. Under the optimized conditions, two expression cassettes ( and ) were successfully transformed into pyrethrum. Polymerase chain reaction (PCR), Southern blotting, reverse-transcription quantitative PCR (RT-qPCR), and histochemical staining confirmed the identity of transgenic plants. PCR and RT-qPCR analyses confirmed the identity of transgenic plants. The transformation efficiency was 0.83% (5 transgenic lines/600 infected explants). The relative concentration of pyrethrins in transformants (OX T0-1: 1.50% or OX T0-2: 1.24%) was higher than that in nontransformed plants (WT: 0.76%). Thus, the genetic transformation system overcame the low regeneration efficiency and integrated a foreign gene into the pyrethrum genome. The new system is a suitable and effective tool for creating high-yielding cultivars of pyrethrum.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants11121575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229838PMC
June 2022

Electroacupuncture of the Baihui and Shenting acupoints for vascular dementia in rats through the miR-81/IL-16/PSD-95 pathway.

Ann Transl Med 2022 May;10(10):540

Department of Anatomy, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.

Background: There is currently no effective treatment for vascular dementia (VaD). Scalp electroacupuncture (EA) has served clinically as an alternative treatment for VaD, but its mechanism is still unclear. In this study, we investigated the effect of EA at the Baihui (GV 20) and Shenting (GV 24) acupoints on spatial learning and memory ability, and the expression level of (), (), and postsynaptic density protein-95 (PSD-95) in the frontal cortex of VaD rats.

Methods: Male Sprague-Dawley rats were randomly divided into four groups, sham, VaD, non-acupuncture (non-AP) and EA group. The VaD model was established by permanent bilateral occlusion of the common carotid arteries. Morris Water Maze was used to assess the rats' spatial learning and memory. Immunochemistry (IHC), quantitative reverse transcription polymerase chain reaction (qRT-PCR), and western blot analysis were performed to detect the expression level of , , and PSD-95. Finally, luciferase assay was used to determine the effect of on expression in PC12 cells.

Results: The space exploration experiment of MWM showed the time and distance of the rat's activities around the platform were decreased in the EA group. Compared to the VaD and non-AP group, the number of terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL)-positive frontal cortical neurons was significantly decreased in EA group. The number of the PSD-95-positive cells and the expression level in the frontal cortical in the EA group was dramatically increased in comparison with the other groups. In the PC12 cell validation experiment, expression level was reduced under the condition of the mimic treatment, while increased in the inhibitor group. The PSD-95 protein level was up-regulated in the small interfering () group compared to the groups with or without oxygen/glucose deprivation/reperfusion (OGD/R) conditions (P<0.05). However, this was abolished by mimic.

Conclusions: In VaD rats, EA may improve spatial learning and memory through miR-81/IL-16/PSD-95 pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-22-2068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201176PMC
May 2022
-->