Publications by authors named "Jing Lu"

1,468 Publications

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Bergapten: A review of its pharmacology, pharmacokinetics, and toxicity.

Phytother Res 2021 Aug 4. Epub 2021 Aug 4.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Bergapten is a natural furocoumarin, also known as 5-methoxypsoralen, and its medicinal value has been paid more and more attention. By sorting out the pharmacological literature of bergapten, we found that bergapten has a wide range of pharmacological effects, including neuroprotection, organ protection, anticancer, antiinflammatory, antimicrobial, and antidiabetes effects. However,bergapten has complex impacts on the hepatic metabolic enzyme. Moreover, pharmacokinetic studies showed that bergapten has higher absolute bioavailability and can cross the blood-brain barrier and has a great potential for treating brain disease, but the mechanism needs further clarification to make greater use of its ability to treat brain diseases. Furthermore, the phototoxicity of bergapten combined with ultraviolet light has always been mentioned. In view of its wide range of pharmacological activities, bergapten is expected to be a potential drug candidate for the treatment of diabetes and diabetes-induced osteoporosis, epilepsy, Alzheimer's disease, depression, and cancer. However, further studies are needed to elucidate its molecular mechanisms and targets. The phototoxicity of bergapten as a side effect should be further avoided. On the other hand, the photoactivation of bergapten in the anticancer aspect can be better utilized.
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http://dx.doi.org/10.1002/ptr.7221DOI Listing
August 2021

Bilobalide: A review of its pharmacology, pharmacokinetics, toxicity, and safety.

Phytother Res 2021 Aug 2. Epub 2021 Aug 2.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Bilobalide is a natural sesquiterpene trilactone from Ginkgo biloba leaves. It has good water solubility and is widely used in food and pharmaceutical fields. In the last decade, a plethora of studies on the pharmacological activities of bilobalide has been conducted and demonstrated that bilobalide possessed an extensive range of pharmacological activities such as neuroprotective, antioxidative, antiinflammatory, anti-ischemic, and cardiovascular protective activities. Pharmacokinetic studies indicated that bilobalide may have the characteristics of rapid absorption, good bioavailability, wide distribution, and slow elimination. This review aims to summarize the advances in pharmacological, pharmacokinetics, toxicity, and safety studies of bilobalide in the last decade with an emphasis on its neuroprotective and antiinflammatory activities, to provide researchers with the latest information and point out the limitations of relevant research at the current stage and the aspects that should be strengthened in future research.
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http://dx.doi.org/10.1002/ptr.7220DOI Listing
August 2021

Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer.

Bioengineered 2021 Dec;12(1):4201-4216

State Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Jiangsu Women and Children Health Hospital, Nanjing, China.

Endometrial cancer (EC) is the most common gynecological malignancy. Recent studies have uncovered miRNA acted a striking role in predicting the prognosis of multiple tumors. Over 500 EC samples were selected from the Cancer Genome Atlas (TCGA) database. Univariate, LASSO and multivariate Cox regression analysis were employed to screen out the prognosis-involved miRNAs. Kaplan-Meier (K-M) and time-dependent receiver operation characteristic (ROC) curves were conducted to reveal survival analysis and assess the accuracy of the signature. The independence of the model was verified via univariate and multivariate Cox regression analysis. Besides, qRT-PCR was conducted to testified the expression of 11 miRNAs in 16 paired tissues. A total of 514 specimens were randomly divided into the training set and the testing set, then an 11 miRNAs-based signature were determined which divided the patients into high-risk group and low-risk group. The survival was markedly different and the ROC curve exhibited a precise prediction. Meanwhile, the univariate and multivariate Cox regression analysis verified the miRNAs-based model was an independent indicator of EC. Moreove, the prediction ability of this model with clinicopathological features was more efficient. Finally, functional enrichment analysis demonstrated these miRNAs were associated with the occurrence and progression of cancer. Additionally, hsa-mir-216b, hsa-mir-363, hsa-mir-940 and hsa-mir-1301 were highly expressed in EC tissues in contrast to normal tissues through qRT-PCR. Importantly, the eleven-miRNA signature was full of robust ability to predict the prognosis of EC.
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http://dx.doi.org/10.1080/21655979.2021.1952051DOI Listing
December 2021

Three New C-Diterpenoid Alkaloids from Aconitum novoluridum.

Chem Pharm Bull (Tokyo) 2021 ;69(8):811-816

School of Life Science and Engineering, Southwest Jiaotong University.

Three new aconitine-type C-diterpenoid alkaloid namely novolunines A (1), B (2), and C (3), along with fifteen known diterpenoid alkaloids were isolated from the roots of Aconitum novoluridum, whose phytochemical investigations have never been reported before. The structures of three new alkaloids were established on the basis of spectra data (high-resolution electrospray ionization (HR-ESI)-MS, IR, one dimensional (1D)- and 2D-NMR). Noteworthily, novolunines A (1) and B (2) are two diterpenoid alkaloids bearing conformational isomerism. In addition, the diterpenoid alkaloids 1-3 did not show any anti-acetylcholinesterase (AChE) or anti-inflammatory activities.
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http://dx.doi.org/10.1248/cpb.c21-00262DOI Listing
January 2021

Single-crystal-to-single-crystal transformations among three Mn-MOFs containing different water molecules induced by reaction time: crystal structures and proton conductivities.

Dalton Trans 2021 Jul 30. Epub 2021 Jul 30.

Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, PR China.

Three Mn-MOFs {[Mn3(μ4-L)2(H2O)7]·4H2O}n (1), {[Mn3(μ5-L)2(H2O)6]·4H2O}n (2) and {[Mn3(μ7-L)2(H2O)2]}n (3) (H3L = 5-(6-carboxypyridin-3-yl)isophthalic acid) were obtained under different reaction times and temperatures. Interestingly, induced by reaction time, compound 1 can lose one water molecule and SC-SC transform into compound 2. Similarly, compound 2 can also SC-SC transform into 3. Studies on two SC-SC transformation processes were carried out and the transformation mechanisms were deduced, which were verified by TG analyses. Different numbers of water molecules in the three compounds resulted in different coordination environments of the metal cation, coordination modes of the L3- ligand, continuities of hydrogen bonds, dimensions of framework and porosities. The AC impendence spectra studies revealed that compounds 1-3 can enhance the proton conductivities of the Nafion composite membrane to about 47.77%, 36.88% and 21.28%, respectively. It is speculated that the highest proton conductivity of compound 1 may be due to its continuous hydrogen bond chain and highest water uptake, which were mainly decided by the number of water molecules.
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http://dx.doi.org/10.1039/d1dt01163dDOI Listing
July 2021

HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Cell Death Dis 2021 Jul 26;12(8):738. Epub 2021 Jul 26.

Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Guangdong Engineering Laboratory of Druggability and New Drug Evaluation, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Sun Yat-sen University, Guangzhou, China.

Heme oxygenase-1 (HO-1) has attracted accumulating attention for its antioxidant enzymatic activity. However, the exact regulatory role of its non-enzymatic activity in the cardiovascular system remains unaddressed. Here, we show that HO-1 was accumulated in the nuclei of stress-induced senescent endothelial cells, and conferred protection against endothelial senescence independent of its enzymatic activity. Overexpression of ΔHO-1, a truncated HO-1 without transmembrane segment (TMS), inhibited HO-induced endothelial senescence. Overexpression of ΔHO-1, the catalytically inactive form of ΔHO-1, also exhibited anti-senescent effect. In addition, infection of recombinant adenovirus encoding ΔHO-1 with three nuclear localization sequences (NLS), alleviated endothelial senescence induced by knockdown of endogenous HO-1 by CRISPR/Cas9. Moreover, repression of HO-1 nuclear translocation by silencing of signal peptide peptidase (SPP), which is responsible for enzymatic cleavage of the TMS of HO-1, exacerbated endothelial senescence. Mechanistically, nuclear HO-1 interacted with NPM1 N-terminal portion, prevented NPM1 translocation from nucleolus to nucleoplasm, thus disrupted NPM1/p53/MDM2 interactions and inhibited p53 activation by NPM1, finally resisted endothelial senescence. This study provides a novel understanding of HO-1 as a promising therapeutic strategy for vascular senescence-related cardiovascular diseases.
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http://dx.doi.org/10.1038/s41419-021-04035-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313700PMC
July 2021

Confined Crystallization and Melting Behaviors of 3-Pentadecylphenol in Anodic Alumina Oxide Nanopores.

ACS Omega 2021 Jul 8;6(28):18235-18247. Epub 2021 Jul 8.

State Key Laboratory of High-Efficiency Coal Utilization and Green Chemical Engineering, Ningxia University, 489 Helanshan West Road, Yinchuan 750021, China.

To explore the effects of end groups on the confined crystallization of an alkyl chain, 3-pentadecylphenol (PDP) was infiltrated into the anodic aluminum oxide template (AAO) to investigate the melting and crystallization behaviors of PDP in a nanoconfined environment. Wide-angle X-ray diffraction (WAXD) found that the solid-solid phase transition of PDP occurred under confined conditions, and the absence of the (00L) reflections indicated that the stacking of the end groups of the alkyl chain layered structure was seriously disturbed. Thermal analysis (TG) showed that the thermal stability of the confined samples decreased due to the confinement effect, and the introduction of end groups made the confinement effect more obvious. Differential scanning calorimeter (DSC) results well reflected the space-time equivalence in the PDP crystallization processes, i.e., the solid-solid phase transition can be achieved by reducing the cooling rate or confining PDP in the nanometer space. Compared with C, the introduction of the end groups with a phenol ring led to the disappearance of the solid-solid phase transition of an alkyl chain at high cooling rates. In the confined environment, the introduction of the end groups with a phenol ring caused the melting double peaks of the alkyl chain to become a single melting peak, and it also caused the disappearance of the surface freezing monolayer for alkyl chains. Through the analysis of crystallinity, it was found that AAO-PDP was more sensitive to AAO pore size changes than AAO-C, the of AAO-PDP had a good linear relationship with the pore size , but the of the AAO-C had a nonlinear relationship with the pore size . Attenuated total reflection (ATR)-IR proved that in the confined environment, the order of the alkyl chain decreased and the degree of chain distortion increased.
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http://dx.doi.org/10.1021/acsomega.1c02112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296606PMC
July 2021

Clinical Feature-Based Machine Learning Model for 1-Year Mortality Risk Prediction of ST-Segment Elevation Myocardial Infarction in Patients with Hyperuricemia: A Retrospective Study.

Comput Math Methods Med 2021 5;2021:7252280. Epub 2021 Jul 5.

Program of Artificial Intelligence in Medicine, College of Medicine, Soochow University, Suzhou 215123, China.

Accurate risk assessment of high-risk patients is essential in clinical practice. However, there is no practical method to predict or monitor the prognosis of patients with ST-segment elevation myocardial infarction (STEMI) complicated by hyperuricemia. We aimed to evaluate the performance of different machine learning models for the prediction of 1-year mortality in STEMI patients with hyperuricemia. We compared five machine learning models (logistic regression, -nearest neighbor, CatBoost, random forest, and XGBoost) with the traditional global (GRACE) risk score for acute coronary event registrations. We registered patients aged >18 years diagnosed with STEMI and hyperuricemia at the Affiliated Hospital of Zunyi Medical University between January 2016 and January 2020. Overall, 656 patients were enrolled (average age, 62.5 ± 13.6 years; 83.6%, male). All patients underwent emergency percutaneous coronary intervention. We evaluated the performance of five machine learning classifiers and the GRACE risk model in predicting 1-year mortality. The area under the curve (AUC) of the six models, including the GRACE risk model, ranged from 0.75 to 0.88. Among all the models, CatBoost had the highest predictive accuracy (0.89), AUC (0.87), precision (0.84), and F1 value (0.44). After hybrid sampling technique optimization, CatBoost had the highest accuracy (0.96), AUC (0.99), precision (0.95), and F1 value (0.97). Machine learning algorithms, especially the CatBoost model, can accurately predict the mortality associated with STEMI complicated by hyperuricemia after a 1-year follow-up.
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http://dx.doi.org/10.1155/2021/7252280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275420PMC
July 2021

Study of membrane fouling mechanism during the phenol degradation in microbial fuel cell and membrane bioreactor coupling system.

Bioresour Technol 2021 Oct 5;338:125504. Epub 2021 Jul 5.

School of the Environment and Safety Engineering, North University of China, Taiyuan 030051, China. Electronic address:

This study evaluated the feasibility of phenol degradation in microbial fuel cell (MFC) and membrane bioreactor (MBR) coupling system, and explored the mechanism of MBR membrane fouling. Four aspects were researched in open and closed circuit conditions: the degradation capacity of the coupling system, the increase of trans-membrane pressure (TMP), and the adhesion of phenol degradation products and microorganisms on the membrane. The results showed that the degradation of phenol and COD in the closed circuit coupling system was higher than that in the open circuit. The micro-electric field can inhibit the growth of TMP and keep dodecamethylcyclohexasiloxane away from the membrane, meanwhile can also reduce the abundance and species diversity of microorganisms. Nevertheless, the micro-electric field could not completely eliminate the membrane fouling due to the fact that the phenol degradation product of ethanethiol, microorganisms of Proteobacteria and Actinobacteria were more favorable on the membrane.
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http://dx.doi.org/10.1016/j.biortech.2021.125504DOI Listing
October 2021

[Ag(NH3)2]2SO4: A strategy for the coordination of cationic moieties to design nonlinear optical materials.

Angew Chem Int Ed Engl 2021 Jul 15. Epub 2021 Jul 15.

Beijing Normal University, College of Chemistry, CHINA.

Over recent decades, guided by the anionic group theory of nonlinear optical (NLO) materials, rational design strategies have been primarily focused on anionic moieties; consequently, structural modification and design of cationic moieties have long been neglected. Herein, we report a strategy for the coordination of cationic moieties that substantially enhances the nonlinear and linear optical properties of NLO materials. For an example with well-known crystal structure, [Ag(NH 3 ) 2 ] 2 SO 4 , we demonstrate that the coordination of the Ag + cation by the neutral ligand drives the formation of a noncentrosymmetric tetragonal P 2 1 c structure as a positive uniaxial crystal. The bending of the [Ag(NH 3 ) 2 ] + cationic moiety parallel to the z- axis generates an anisotropic arrangement of the dipoles, i.e., a dipole of 0.12 D along the z -direction, yet zero dipole in the xy -plane, which interacts anisotropically with the incident light oscillating electromagnetic wave, leading to optical anisotropy with a large birefringence. The incident beam of 589.3 nm normal to the (110) crystal plane measures ∆ n obv. = 0.08, and [Ag(NH 3 ) 2 ] 2 SO 4 also exhibits a phase-matching NLO response 1.4 times that of KH 2 PO 4 (KDP) ( obv . 1.4 × KDP @1064 nm; cal . d 36 = 1.50 pm/V). These discoveries will illuminate future explorations of other functional ionic materials.
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http://dx.doi.org/10.1002/anie.202107780DOI Listing
July 2021

Associations Between Obesity and Kidney Disease in Chinese Men and Women With Type 2 Diabetes: A Retrospective Cohort Study.

Can J Diabetes 2021 May 19. Epub 2021 May 19.

Department of Endocrinology, Drum Tower Clinical College of Nanjing Medical University, Nanjing, China; Department of Endocrinology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China. Electronic address:

Background: Our aim in this study was to explore the risk factors for kidney disease in Chinese men and women with type 2 diabetes (T2D) and to clarify the relationship between obesity and the risk of chronic kidney disease (CKD).

Methods: This retrospective study included 3,194 patients with T2D. Among 2,574 T2D patients without CKD at baseline, 753 with follow-up records of at least 12 months were included in the retrospective cohort. Logistic regression and Cox regression were used to evaluate the risk for CKD in men and women. A restricted cubic spline model was used to analyze the association of body mass index (BMI) and waist circumference (WC) with CKD risk.

Results: Multivariate logistic regression analysis suggested that obesity was a risk factor for T2D with CKD in men but not in women. After a median follow-up period of 2.8 years, the incidence of CKD in men with obesity was significantly higher than that in men with obesity with T2D (p=0.039), but there was no statistically significant difference between women with obesity and women without obesity with T2D (p=0.825). In the restricted cubic spline model analysis, BMI and WC were associated with CKD risk in a nonlinear fashion in males and females. The risk of CKD was higher in males with a BMI of ≥29.5 kg/m or a WC of ≥100 cm, whereas there was no difference observed in females.

Conclusions: Obesity was strongly related to T2D with CKD in men. Male diabetes patients with obesity, especially abdominal obesity, are more likely to develop CKD.
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http://dx.doi.org/10.1016/j.jcjd.2021.05.005DOI Listing
May 2021

Ablation characteristics and material removal mechanisms of a single-crystal diamond processed by nanosecond or picosecond lasers.

Opt Express 2021 Jul;29(14):22714-22731

The microstructures on a diamond surface have attracted extensive attention in microelectronics, ultra-precision machining tools, and optical elements, etc. In this work, microgrooves were fabricated on a single-crystal diamond surface using ultraviolet nanosecond or infrared picosecond laser pulses. The surface and internal morphologies of the microgrooves were characterized. The chemical composition and phase transition of the diamond after laser irradiation were analyzed. Furthermore, the ablation threshold, ablation rate, and material removal rate of the diamond processed by nanosecond or picosecond lasers were also calculated. In addition, the temperature distributions of the diamond ablated by nanosecond or picosecond lasers were simulated. Finally, the material removal mechanisms of a single-crystal diamond processed by nanosecond or picosecond lasers were revealed. This work is expected helpful to provide a guidance for the laser fabrication of microstructures on diamond.
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http://dx.doi.org/10.1364/OE.430354DOI Listing
July 2021

Improved Auditory Function Caused by Music Versus Foreign Language Training at School Age: Is There a Difference?

Cereb Cortex 2021 Jul 16. Epub 2021 Jul 16.

State Key Laboratory of Cognitive Neuroscience and Learning and IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China.

In adults, music and speech share many neurocognitive functions, but how do they interact in a developing brain? We compared the effects of music and foreign language training on auditory neurocognition in Chinese children aged 8-11 years. We delivered group-based training programs in music and foreign language using a randomized controlled trial. A passive control group was also included. Before and after these year-long extracurricular programs, auditory event-related potentials were recorded (n = 123 and 85 before and after the program, respectively). Through these recordings, we probed early auditory predictive brain processes. To our surprise, the language program facilitated the children's early auditory predictive brain processes significantly more than did the music program. This facilitation was most evident in pitch encoding when the experimental paradigm was musically relevant. When these processes were probed by a paradigm more focused on basic sound features, we found early predictive pitch encoding to be facilitated by music training. Thus, a foreign language program is able to foster auditory and music neurocognition, at least in tonal language speakers, in a manner comparable to that by a music program. Our results support the tight coupling of musical and linguistic brain functions also in the developing brain.
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http://dx.doi.org/10.1093/cercor/bhab194DOI Listing
July 2021

Improvement of alkali metal ion batteries via interlayer engineering of anodes: from graphite to graphene.

Nanoscale 2021 Aug 15;13(29):12521-12533. Epub 2021 Jul 15.

State Key Laboratory for Mesoscopic Physics and Department of Physics, Peking University, Beijing 100871, P. R. China.

Interlayer engineering of graphite anodes in alkali metal ion (M = Li, Na, and K) batteries is carried out based on the first-principles calculations. By increasing the interlayer spacing of graphite, the specific capacity of Li or Na does not increase while that of K increases continuously (from 279 mA h g at the equilibrium interlayer spacing to 1396 mA h g at the interlayer spacing of 20.0 Å). As the interlayer spacing increases, the electrostatic potential of graphite becomes smoother, and the ability to buffer the electrostatic potential fluctuation becomes poorer in M ions. These two effects jointly lead to minima of the diffusion barrier of M ions on graphite (0.01-0.05 eV), instead of strictly monotonous declines with the increasing interlayer spacing. To perform the interlayer engineering of anode candidates more efficiently, a set of high-throughput programs has been developed and can be easily applied to other systems. Our research has guiding significance for achieving the optimal effect in interlayer engineering experimentally.
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http://dx.doi.org/10.1039/d1nr01946eDOI Listing
August 2021

Rapid detection of using a DNAzyme-based sensor.

Food Sci Nutr 2021 Jul 11;9(7):3873-3884. Epub 2021 Jun 11.

Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology Jiangsu Ocean University Lianyungang China.

In the present study, a DNAzyme was screened in vitro through the use of a DNA library and crude extracellular mixture (CEM) of . Following eight rounds of selection, a DNAzyme termed PAE-1 was obtained, which displayed high rates of cleavage with strong specificity. A fluorescent biosensor was designed for the detection of in combination with the DNAzyme. A detection limit as low as 1.2 cfu/ml was observed. Using proteases and filtration, it was determined that the target was a protein with a molecular weight of 10 kDa-50 kDa. The DNAzyme was combined with a polystyrene board to construct a simple indicator plate sensor which produced a color that identified the target within 10 min. The results were reliable when tap water and food samples were tested. The present study provides a novel experimental strategy for the development of sensors based on a DNAzyme to rapidly detect in the field.
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http://dx.doi.org/10.1002/fsn3.2367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269565PMC
July 2021

Achieving the HbA1c Target Requires Longer Time in Range in Pregnant Women with Type 1 Diabetes.

J Clin Endocrinol Metab 2021 Jul 9. Epub 2021 Jul 9.

Department of Endocrinology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Aims: Continuous glucose monitoring (CGM) overcomes the limitations of glycated hemoglobin (HbA1c). This study was to investigate the relationship between CGM metrics and laboratory HbA1c in pregnant women with type 1 diabetes.

Methods: An observational study enrolled pregnant women with type 1 diabetes who wore CGM devices during pregnancy and postpartum from 11 hospitals in China from January 2015 to June 2019. CGM data were collected to calculate time-in-range (TIR), time above range (TAR), time below range (TBR), and glycemic variability parameters. Relationships between the CGM metrics and HbA1c were explored. Linear and curvilinear regressions were conducted to investigate the best-fitting model to clarify the influence of HbA1c on the TIR-HbA1c relationship during pregnancy.

Results: A total of 272 CGM data and corresponding HbA1c from 98 pregnant women with type 1 diabetes and their clinical characteristics were analyzed in this study. Mean HbA1c and TIR were 6.49±1.29% and 76.16±17.97% during pregnancy, respectively. HbA1c was moderately correlated with TIR 3.5-7.8(R= -0.429, P=0.001), mean glucose (R= 0.405, P=0.001) and TAR 7.8 (R=0.435, P=0.001), but was weakly correlated with TBR 3.5 (R=0.034, P=0.001) during pregnancy. On average, a 1% (11 mmol/mol) decrease in HbA1c corresponded to an 8.5% increase in TIR 3.5-7.8. During pregnancy, HbA1c of 6.0%, 6.5% and 7.0% were equivalent to a TIR 3.5-7.8 of 78%, 74%, and 69%, respectively.

Conclusions: We found that there was a moderate correlation between HbA1c and TIR 3.5-7.8 during pregnancy. To achieve the HbA1c target <6.0%, pregnant women with type 1 diabetes should strive for TIR 3.5-7.8 >78% (18h 43min) during pregnancy.
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http://dx.doi.org/10.1210/clinem/dgab502DOI Listing
July 2021

Decomposition of rock micro-fracture signals based on a singular value empirical mode decomposition algorithm.

Rev Sci Instrum 2021 May;92(5):055102

Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory, School of Information Engineering, Southwest University of Science and Technology, Mianyang 621010, China.

Rock burst early warning technology is currently applied mainly in microseismic monitoring. Rock burst signals indicate the micro-fracture phenomena of a rock and can transmit earthquake waves through the rock before they are finally received by a detector. A characteristic decomposition of rock micro-fracture signals was conducted by the singular value Empirical Mode Decomposition (EMD) algorithm to effectively decompose the characteristic signals of a rock micro-fracture from mixed microseismic signals, with a low signal to noise ratio to ensure prediction precision. When comparing the proposed method with wavelet decomposition and EMD, it was found that the local characteristics of the signals were retained effectively. The proposed algorithm was verified by applying it in a laboratory simulation and to the decomposition of microseismic signals from a hydro-power station. It was concluded that the improved algorithm had a better decomposition precision than wavelet decomposition and EMD decomposition and could effectively separate the characteristic signals of micro-earthquakes. This could provide a significant basis for the identification of the abnormal microseismic signals of rock micro-fractures as well as a pre-warning of rock fractures. It is therefore of practical significance to study rock fracture early warning technology.
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http://dx.doi.org/10.1063/5.0048419DOI Listing
May 2021

Theoretical explanation of uneven frequency response of time-domain acoustic contrast control method.

Authors:
Meiling Hu Jing Lu

J Acoust Soc Am 2021 Jun;149(6):4292

Key Laboratory of Modern Acoustics, Institute of Acoustics, Nanjing University, Nanjing 210093, China.

Acoustic contrast control (ACC) is one of the most commonly utilized sound zone control methods, which maximizes the ratio of the average squared sound pressure in two control zones. The time-domain formulation of ACC (TACC) is usually preferred since it can optimize the whole bandwidth in a single step and the resulting control filter is guaranteed to be causal. Many researchers have noted that TACC suffers from uneven frequency response. However, a convincing theoretical explanation of this problem is yet to be investigated. In this letter, an asymptotically equivalent frequency-domain form of TACC is constructed, based on which it can be proven that TACC has the tendency of extracting the specific frequency component with the highest contrast. Simulation results validate the efficacy of this theoretical analysis.
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http://dx.doi.org/10.1121/10.0005270DOI Listing
June 2021

Prediction of non-reassuring fetal status and umbilical artery acidosis by the maternal characteristic and ultrasound prior to induction of labor.

BMC Pregnancy Childbirth 2021 Jul 6;21(1):489. Epub 2021 Jul 6.

Department of Obstetrics and Gynaecology, Fujian Province, The First Affiliated Hospital of Xiamen University, No.55 Zhenhai Road, Xiamen City, 351000, China.

Objective: To investigate the predictive value of pre-induction digital examination, sonographic measurements and parity for the prediction of non-reassuring fetal status and cord arterial pH < 7.2 prior to the induction of labor (IOL).

Method: This was a prospective observational study, including 384 term pregnancies undergoing IOL. Before the IOL, the Bishop score (BS) by digital examination, sonographic Doppler parameters and the estimated fetal weight (EFW) was assessed. The fetal cord arterial was sampled to measure the pH at delivery. Multivariate logistic regression analysis was performed to identify independent predictors of non-reassuring fetal status and low cord arterial pH.

Results: Forty four cases (11.5%) had non-reassuring fetal status, and 76 cases (19.8%) had fetal cord arterial pH < 7.2. In the non-reassuring fetal status group, the incidence of cord arterial pH < 7.2 was significantly higher than that in the normal fetal heart rate group (χ = 6.401, p = 0.011). Multivariate analysis indicated that significant independent predictors of non-reassuring fetal status were nulliparity (adjusted odds ratio [AOR]: 3.746, p = 0.003), EFW < 10 percentile (AOR: 3.764, p = 0.003) and cerebroplacental ratio (CPR) < 10 centile (AOR:4.755, p < 0.001). In the prediction of non-reassuring fetal status, the performance of the combination of nulliparity and EFW < 10th percentile was improved by the addition of CPR < 10th percentile (AUC: 0.681, (95%CI: 0.636 to 0.742) vs 0.756, (95%CI:0.713 to 0.795)), but the difference was not significant (DeLong test: z = 1.039, p = 0.053).. None of the above variables were predictors of cord arterial pH < 7.2.

Conclusion: The risk of fetal acidosis has increased in cases of non-reassuring fetal status. Nulliparity, small for gestational age and CPR < 10th centile are independent predictors for non-reassuring fetal status in term fetuses, though the addition of CPR < 10th centile could not significantly improve the screening accuracy.
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http://dx.doi.org/10.1186/s12884-021-03972-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261974PMC
July 2021

Comprehensive Human Milk Patterns Are Related to Infant Growth and Allergy in the CHMP Study.

Mol Nutr Food Res 2021 Jul 6:e2100011. Epub 2021 Jul 6.

Nutrition and Metabolism Research Division, Innovation Center, Heilongjiang Feihe Dairy Co., Ltd., Beijing, 100015, China.

Scope: The aim of the present study is to identify human milk pattern using multi-omics datasets and to explore association between patterns, infant growth, and allergy using data from the Chinese Human Milk Project (CHMP) study.

Methods And Results: Three patterns are identified from integrative analysis of proteome, lipidome, and glycome profiles of 143 mature human milk samples. Factor 1 is positively associated with 128 proteins, phospholipids, and human milk oligosaccharides (HMOs) including lacto N-neohexaose (LNnH) and lacto-N-difucohexaose II (LNDFH II); factor 2 is negatively associated with as -casein, phospholipids while positively associates with HMOs including LNnH, lactosialyl tetrasaccharide c (LSTc), and 2'-fucosyllactose (2'FL); factor 3 is positively associated with lysophospholipids while negatively associates with 27 proteins, triglycerides with two saturated fatty acids, 6'-sialyllactose (6'SL) and 2'FL. In general, factor 1 and factor 2 are associated with slower while factor 3 is associated with faster growth rate (p < 0.044). One unit higher in loadings of factor 2 is associated with 34% lower risk of allergies (p ≤ 0.017). Associations are not significant after adjustment for city except for factor 1.

Conclusions: Three possible human milk patterns with varying degree of stability are identified. Future work is needed to understand these patterns in terms of generalization, biologic mechanisms, and genotype influences.
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http://dx.doi.org/10.1002/mnfr.202100011DOI Listing
July 2021

Cloperastine inhibits esophageal squamous cell carcinoma proliferation in vivo and in vitro by suppressing mitochondrial oxidative phosphorylation.

Cell Death Discov 2021 Jun 21;7(1):166. Epub 2021 Jun 21.

The Pathophysiology Department, School of Basic Medical Sciences, College of Medicine, Zhengzhou University, Zhengzhou, 450000, China.

Esophageal squamous cell carcinoma (ESCC) is a major type of esophageal cancer. The prognosis of patients with ESCC remains poor because of the high morbidity and mortality of the disease. One strategy for drug discovery for ESCC treatment or prevention is screening FDA-approved drugs. In the present study, we found that the antitussive agent cloperastine can inhibit the proliferation of ESCC cells. However, the underlying mechanism was unclear. To determine the mechanism of this inhibitory effect, we performed proteomic analysis using KYSE150 cells treated with cloperastine and DMSO. The results identified several down-regulated signaling pathways included those of three key proteins (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex 1, NADH ubiquinone oxidoreductase subunit S5, and cytochrome C oxidase subunit 6B1) involved in oxidative phosphorylation. Meanwhile, we observed that oxidative phosphorylation in mitochondria was inhibited by the drug. Importantly, cloperastine suppressed ESCC growth in a xenograft mouse model in vivo. Our findings revealed that cloperastine inhibits the proliferation of ESCC in vivo and in vitro by suppressing mitochondrial oxidative phosphorylation.
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http://dx.doi.org/10.1038/s41420-021-00509-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257628PMC
June 2021

Can Carbon Nanotube Transistors Be Scaled Down to the Sub-5 nm Gate Length?

ACS Appl Mater Interfaces 2021 Jul 2;13(27):31957-31967. Epub 2021 Jul 2.

Key Laboratory for the Physics and Chemistry of Nanodevices and Department of Electronics and Center for Carbon-based Electronics, Peking University, Beijing 100871, P. R. China.

Single-walled carbon nanotubes (CNTs) have been considered as a promising semiconductor to construct transistors and integrated circuits in the future owing to their ultrathin channel thickness and ultrahigh injection velocity. Although a 5 nm gate-length CNT field-effect transistor (FET) has already been experimentally fabricated and demonstrates excellent device performance, the potential or constraint factors on performance have not been explored or revealed. Based on the benchmark of the device performance between the experimental and simulated 5 nm gate-length CNT FETs, we use the first-principles quantum transport approach to explore the performance limit of CNT FETs based on the gate-all-around (GAA) device geometry for the first time. It is found that the GAA CNT FETs can fulfill the ITRS 2028 high-performance target in the 2 nm gate-length node in terms of the on-state current, delay time, and power consumption. We also find that the energy-delay product of the CNT FETs is superior to those of the high-performance 2D materials and Si Fin FETs at the sub-5 nm gate length due to its unique electrical property. Though theoretically the gate length of CNT FETs can be potentially scaled to 2 nm, considering the tradeoff between the performance and power consumption, 5 nm is the ultimate scaled limit.
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http://dx.doi.org/10.1021/acsami.1c05229DOI Listing
July 2021

Intra-heterogeneity in transcription and chemoresistant property of leukemia-initiating cells in murine Setd2 acute myeloid leukemia.

Cancer Commun (Lond) 2021 Jul 1. Epub 2021 Jul 1.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, P. R. China.

Background: Heterogeneity of leukemia-initiating cells (LICs) is a major obstacle in acute myeloid leukemia (AML) therapy. Accumulated evidence indicates that the coexistence of multiple types of LICs with different pathogenicity in the same individual is a common feature in AML. However, the functional heterogeneity including the drug response of coexistent LICs remains unclear. Therefore, this study aimed to clarify the intra-heterogeneity in LICs that can help predict leukemia behavior and develop more effective treatments.

Methods: Spleen cells from the primary Setd2 -AML mouse were transplanted into C57BL/6 recipient mice to generate a transplantable model. Flow cytometry was used to analyze the immunophenotype of the leukemic mice. Whole-genome sequencing was conducted to detect secondary hits responsible for leukemia transformation. A serial transplantation assay was used to determine the self-renewal potential of Setd2 -AML cells. A limiting-dilution assay was performed to identify the LIC frequency in different subsets of leukemia cells. Bulk and single-cell RNA sequencing were performed to analyze the transcriptional heterogeneity of LICs. Small molecular inhibitor screening and in vivo drug treatment were employed to clarify the difference in drug response between the different subsets of LICs.

Results: In this study, we observed an aged Setd2 mouse developing AML with co-mutation of Nras and Braf . Further investigation identified two types of LICs residing in the c-Kit B220 Mac-1 and c-Kit B220 Mac-1 subsets, respectively. In vivo transplantation assay disclosed the heterogeneity in differentiation between the coexistent LICs. Besides, an intrinsic doxorubicin-resistant transcriptional signature was uncovered in c-Kit B220 Mac-1 cells. Indeed, doxorubicin plus cytarabine (DA), the standard chemotherapeutic regimen used in AML treatment, could specifically kill c-Kit B220 Mac-1 cells, but it hardly affected c-Kit B220 Mac-1 cells. Transcriptome analysis unveiled a higher activation of RAS downstream signaling pathways in c-Kit B220 Mac-1 cells than in c-Kit B220 Mac-1 cells. Combined treatment with DA and RAS pathway inhibitors killed both c-Kit B220 Mac-1 and c-Kit B220 Mac-1 cells and attenuated disease progression.

Conclusions: This study identified two cell subsets enriched for LICs in murine Setd2 -AML and disclosed the transcriptional and functional heterogeneity of LICs, revealing that the coexistence of different types of LICs in this model brings about diverse drug response.
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http://dx.doi.org/10.1002/cac2.12189DOI Listing
July 2021

Sodium Sulfite-Induced Mast Cell Pyroptosis and Degranulation.

J Agric Food Chem 2021 Jul 30;69(27):7755-7764. Epub 2021 Jun 30.

College of Food science and Engineering, Jilin University, Changchun, Jilin 130062, People's Republic of China.

Sodium sulfite, a common food additive, has been proved to cause allergic reaction. Pyroptosis is an inflammatory form of programmed cell death with plasma membrane lysis. In this study, we found that sodium sulfite triggered pyroptosis, which depended on reactive oxygen species (ROS)/NOD-like receptor protein 3 (NLRP3) in RBL-2H3 mast cells. Sodium sulfite increased the generation of ROS and the expression of NLRP3, caspase-1, gasdermin D N-terminal (GSDMD-N), interleukin-1β (IL-1β), and interleukin-18 (IL-18). The ROS scavenger N-acetyl-L-carnosine (NAC) and the NLRP3 inhibitor MCC950 reversed these effects. Furthermore, using a lactate dehydrogenase kit, propidium iodide staining, scanning electron microscopy, colocalization of GSDMD-N with histamine, and neutral red staining, we found that sodium sulfite notably induced cell membrane rupture. Because β-Hexosaminidase and histamine play a key role in allergic reactions, we detected the release of β-Hexosaminidase and histamine. The data showed that the release of β-Hexosaminidase and histamine induced by sodium sulfite was increased with dose independence, which were inhibited after treatment with NAC or MCC950. Overall, evidence suggested that pyroptosis induced by sodium sulfite may rupture the cell membrane and result in degranulation of mast cells. Our study may provide new insights for the mechanism by which sodium sulfite induces mast cell death and sensitization.
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http://dx.doi.org/10.1021/acs.jafc.1c02436DOI Listing
July 2021

[Research on entity relationship extraction of Chinese medical literature and application in diabetes medical literature].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Jun;38(3):563-573

College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen, Guangdong 518118, P.R.China.

The medical literature contains a wealth of valuable medical knowledge. At present, the research on extraction of entity relationship in medical literature has made great progress, but with the exponential increase in the number of medical literature, the annotation of medical text has become a big problem. In order to solve the problem of manual annotation time such as consuming and heavy workload, a remote monitoring annotation method is proposed, but this method will introduce a lot of noise. In this paper, a novel neural network structure based on convolutional neural network is proposed, which can solve a large number of noise problems. The model can use the multi-window convolutional neural network to automatically extract sentence features. After the sentence vectors are obtained, the sentences that are effective to the real relationship are selected through the attention mechanism. In particular, an entity type (ET) embedding method is proposed for relationship classification by adding entity type characteristics. The attention mechanism at sentence level is proposed for relation extraction in allusion to the unavoidable labeling errors in training texts. We conducted an experiment using 968 medical references on diabetes, and the results showed that compared with the baseline model, the present model achieved good results in the medical literature, and F1-score reached 93.15%. Finally, the extracted 11 types of relationships were stored as triples, and these triples were used to create a medical map of complex relationships with 33 347 nodes and 43 686 relationship edges. Experimental results show that the algorithm used in this paper is superior to the optimal reference system for relationship extraction.
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http://dx.doi.org/10.7507/1001-5515.202001009DOI Listing
June 2021

Serum IL-35 is decreased in overweight patients with rheumatoid arthritis: its correlation with Th1/Th2/Th17-related cytokines.

BMC Immunol 2021 Jun 27;22(1):42. Epub 2021 Jun 27.

Department of Rheumatology and Immunology, The First Affiliated Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang, 110001, PR China.

Background: Obesity is correlated with worse drug responses and high disease activity in patients with rheumatoid arthritis (RA). Interleukin (IL)-35 is a novel anti-inflammatory cytokine that mainly produced by regulatory T (Treg). This study was performed to analyze whether IL-35 was correlated with obesity in RA and investigate the correlation between other Th1/Th2/Th17-related cytokines and obesity in RA.

Results: The serum IL-35 level was analyzed in RA (n = 81) and healthy donors (n = 53) by ELISA assay, and was compared between three groups (body mass index (BMI) < 18.5,≥18.5 to 25, > 25). Serum cytokines including IL-2, IL-4, IL-10, IL-17, INF-γ, TNF-α levels were measured using Flowcytometry assay. Clinical information was extracted from medical records. Serum IL-35 level in overweight patients were significantly decreased than those in lean patients. Furthermore, Th1/Th2/Th17-related cytokines from overweight patients with RA showed the characteristic immunological features. Serum IL-6, IL-17 and TNF-α levels were positively correlated with BMI. However, serum IL-2, IL-4, IL-10 and IFN-γ concentrations were not correlated with BMI.

Conclusions: Quantitative changes in serum IL-35 level were characteristic in overweight patients with RA. These findings indicate that IL-35 plays an important role in the development of RA and may prove to be a potential biomarker of active RA.
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http://dx.doi.org/10.1186/s12865-021-00431-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237483PMC
June 2021

Lidocaine-eluting endotracheal tube effectively attenuates intubation related airway response.

Ann Transl Med 2021 May;9(10):871

Department of Anesthesiology, Laboratory of Anesthesia & Critical Care Medicine, Translational Neuroscience Center, West China Hospital of Sichuan University, Chengdu, China.

Background: Lidocaine (LDC) is a local anesthetic widely used to relieve intubation-related airway responses. However, low drug concentration and short effective duration of LDC is inadequate to provide a satisfactory anesthetic effect on the surface of the airway. The present study sought to develop a LDC-delivery endotracheal tube (ETT) to achieve high local drug concentration and sustained drug release with the aim of attenuating an intubation-related airway response.

Methods: ETTs and polyvinyl chloride (PVC) discs were coated with different molecular weight (MW) poly lactic-co-glycolic acid (PLGA: 50/50; MW: 3,000, 6,000, and 10,000) loaded with LDC by airbrush spray. The morphology of LDC-eluting coatings was analyzed using scanning electron microscopy. drug release was determined by ultraviolet spectrophotometer. An study was performed to investigate the differences in plasma LDC concentration, intubation tolerance, and tracheal tissue injury in rabbits undergoing intubation of blank, LDC-spray, or LDC-coated ETTs.

Results: Approximate 5 mg/cm coatings (containing 2.5 mg/cm LDC) were deposited onto the PVC discs and ETTs. While even distribution and smooth surfaces were generated in PLGA3000 + LDC and PLGA6000 + LDC coatings, PLGA10000 + LDC formed uneven and gullied coatings. Burst release within the first 4 h and sustained release for at least 5 days was achieved in PLGA + LDC coatings and the study demonstrated higher plasma LDC concentration and longer drug release duration in LDC-coated ETTs compared with LDC-spray. LDC-coated ETTs significantly improved intubation tolerance in rabbits, as measured by less general anesthetic consumption and longer tube tolerance duration in contrast to blank ETTs with or without LDC spray. Histology assessment showed less mucosal edema area in the PLGA3000 + LDC and PLGA6000 + LDC groups compared to the control, LDC-spray, and PLGA10000 + LDC groups. Among the different MW PLGAs, PLGA6000 presented optimal morphological characteristics, drug release, and anesthetic effect.

Conclusions: ETTs coated with PLGA + LDC effectively attenuate an intubation-related airway response increasing local drug concentration and extending drug action duration, which demonstrates a potential therapeutic benefit for patients undergoing intubation.
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http://dx.doi.org/10.21037/atm-21-1930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184491PMC
May 2021

Novel Mathematical Model for Transient Pressure Analysis of Multifractured Horizontal Wells in Naturally Fractured Oil Reservoirs.

ACS Omega 2021 Jun 31;6(23):15205-15221. Epub 2021 May 31.

Petroleum Engineering Department, Khalifa University of Science and Technology, P.O. Box 2533, Abu Dhabi, United Arab Emirates.

Multifractured horizontal wells have gained significant attention within the petroleum industry for commercial development. Despite considerable developments of transient pressure analysis or flow rate behaviors for horizontal wells in naturally fractured reservoirs, some significant problems are yet to be resolved, including high heterogeneity of reservoirs, pressure sensitivity of hydraulic fractures, and non-Darcy flow effect, which may occur during the production life. This paper presents a more pragmatic mathematical model for multifractured horizontal wells in naturally fractured reservoirs based on the fractal system, the theory of permeability modulus, and the time-fractional calculus correspondingly as an extension of the classic trilinear flow model. This new model comprises three modules: high heterogeneity of the reservoir based on the fractal system, the permeability modulus typically showing the pressure sensitivity of hydraulic fractures, and the anomalous diffusion describing non-Darcy flow turbulence. This investigation evaluates a trilinear dual-permeability dual-porosity flow model, with the dual-porosity model for the unstimulated outer reservoir flow region, the dual-permeability model for the stimulated inner reservoir flow region, and the permeability modulus for the flow region of hydraulic fractures. The comprehensive sensitivity analysis conducted indicates how the key parameters, such as fractal dimension, hydraulic fracture permeability modulus and conductivity, interporosity flow coefficient, storativity ratio, etc., affect the transient pressure behaviors, along with their reasons for the change in behavior. Application to a field case study further demonstrates the validity of the mathematical model, and the results presented may play a guiding role in well test interpretation.
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http://dx.doi.org/10.1021/acsomega.1c01464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210455PMC
June 2021

Detection of in Seafood With a DNAzyme-Based Biosensor.

Front Microbiol 2021 4;12:655845. Epub 2021 Jun 4.

Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China.

is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the detection of . After screening and mutation, a DNAzyme that we denominated "RFD-VV-M2" exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 10 CFU/ml, and results could be obtained within 5-10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with . Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for in aquatic products.
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http://dx.doi.org/10.3389/fmicb.2021.655845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213197PMC
June 2021
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