Publications by authors named "Jing Li"

12,485 Publications

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Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.

Cell Rep 2022 May 8:110864. Epub 2022 May 8.

Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, Yunnan 650118, China; Key Laboratory of Systemic Innovative Research on Virus Vaccine, Chinese Academy of Medical Sciences, Kunming, Yunnan 650118, China. Electronic address:

The pathological and immune response of individuals with COVID-19 display different dynamics in lung and intestine. Here, we depict the single-cell transcriptional atlas of longitudinally collected lung and intestinal tissue samples from SARS-CoV-2-infected monkeys at 3 to 10 dpi. We find that intestinal enterocytes are degraded at 3 days post-infection but recovered rapidly, revealing that infection has mild effects on the intestine. Crucially, we observe suppression of the inflammatory response and tissue damage related to B-cell and Paneth cell accumulation in the intestines, although T cells are activated in the SARS-CoV-2 infection. Compared with that in the lung, the expression of interferon response-related genes is inhibited, and inflammatory factor secretion is reduced in the intestines. Our findings indicate an imbalance of immune dynamic in intestinal mucosa during SARS-CoV-2 infection, which may underlie ongoing rectal viral shedding and mild tissue damage.
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http://dx.doi.org/10.1016/j.celrep.2022.110864DOI Listing
May 2022

High-throughput sequencing reveals the main drivers of niche-differentiation of bacterial community in the surface sediments of the northern South China sea.

Mar Environ Res 2022 May 4;178:105641. Epub 2022 May 4.

Environmental Engineering, Guangdong Technion - Israel Institute of Technology, 241 Daxue Road, Shantou, Guangdong, 515063, China; Guangdong Provincial Key Laboratory of Materials and Technologies for Energy Conversion, Guangdong Technion - Israel Institute of Technology, 241 Daxue Road, Shantou, Guangdong, 515063, China. Electronic address:

Studies on marine bacterial communities have revealed endemism in local communities, yet the underlying mechanisms remained elusive. Environmental gradient settings can benefit the straightaway study of community composition changes and the mechanisms explaining them. Here, MiSeq-based 16S rRNA gene sequencing was performed on 12 surface sediment samples from the northern South China Sea (nSCS) revealing that shallow-sea samples had a higher alpha diversity than deep-sea samples, and were differentiated from them significantly based on beta diversity. Temperature, seawater depth, and salinity were the top three influential factors. Bacterial 16S rRNA gene abundance was positively correlated with temperature, and negatively correlated with salinity. Sulfate-reducing bacteria including Desulfobacteraceae, Desulfobulbaceae, and Syntrophobacteraceae were enriched in shallow-sea sediments, co-abundant with nitrite-oxidizing Nitrospira and potential sulfur-oxidizing shallow-sea specific Woeseiaceae/JTB255 clade. Meanwhile, the co-existing and co-abundant of marine anammox and n-damo bacteria were enriched in deep-sea sediments, which was firstly evidenced in this study. The global deep-sea cosmopolitans, OM1 clade, and deep-sea specific Woeseiaceae/JTB255 clade were also found enriched in deep-sea sediments of nSCS. The discovery of novel taxa which were differentially enriched in shallow-/deep-sea sediments not only shed light on enigmatic physiological properties and the natural selection mechanism, but also provided the potential ecological-functional links which invoked further genomics-based metabolic characteristics.
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http://dx.doi.org/10.1016/j.marenvres.2022.105641DOI Listing
May 2022

Questionnaires Measuring Patient Participation in Patient Safety-a systematic review.

J Nurs Manag 2022 May 20. Epub 2022 May 20.

Department of Nursing, West China Hospital, Sichuan University/West China School of Nursing, Sichuan University, Chengdu, Sichuan, P.R. China.

Aim: The purpose of this review was to evaluate the content, validity and reliability of patient-reported questionnaires on patient participation in patient safety.

Background: Patient participation in patient safety is one of the key strategies that are increasingly regarded as a critical intervention to improve the quality of safety care.

Evaluation: A systematic review was conducted according to PRISMA guidelines. The content, reliability and validity of patient-reported questionnaires on patient participation in patient safety were assessed.

Key Issues: Twenty-seven studies were included for data extraction and synthesis. The questionnaire contents most commonly used to describe patient participation in patient safety were 'attitudes and perceptions', 'experience', 'information and feedback' and 'willingness'. Internal consistency was evaluated for seventeen questionnaires, and test-retest reliability was tested for four questionnaires. Content validity was assessed among all included questionnaires, and structural validity was evaluated for twelve questionnaires.

Conclusions: Future research targeting the different safety issues is still indispensable for developing patient-reported questionnaires with great psychometric quality in validity, reliability, feasibility and usability in patient participation in patient safety.

Implications For Nursing Management: Clinical nurses should consider the internal consistency, test-retest reliability, content validity and structural validity of the questionnaires that have been positively appraised for methodological quality before use.
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http://dx.doi.org/10.1111/jonm.13690DOI Listing
May 2022

A new type of δ-vinylvalerolactone for palladium-catalyzed cycloaddition: synthesis of nine-membered heterocycles.

Chem Commun (Camb) 2022 May 20. Epub 2022 May 20.

Department of Chemistry and Innovation Center of Pesticide Research, China Agricultural University, Beijing 100193, China.

In this paper, a new type of δ-vinylvalerolactone was designed and synthesized, and used as a new precursor in Pd-catalyzed [6+3] cycloaddition with azomethine imines, leading to nine-membered 1,2-dinitrogen-containing heterocycles in 77-98% yields with >20 : 1 d.r. These nine-membered ring-fused products were further transformed into unusual tetracyclic bridged-ring compounds without loss of the diastereoselectivities.
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http://dx.doi.org/10.1039/d2cc01134dDOI Listing
May 2022

Sialin mediates submandibular gland regeneration ability by affecting polysialic acid synthesis.

Oral Dis 2022 May 20. Epub 2022 May 20.

Department of Oral and Maxillofacial & Head and Neck Oncology, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.

Objectives: Sialin is a multifunctional molecule with a well-described role in physiological equilibrium regulation. The aim of this study was to elucidate the role of sialin in salivary glands regeneration.

Materials And Methods: Submandibular gland duct ligation/deligation of rat was performed to develop a rat model of submandibular gland regeneration. Phenotype changes were investigated using western blotting and quantitative real-time polymerase chain reaction, as well as immunohistochemical staining. LV-slc17a5-RNAi vectors were injected into the submandibular glands via retroductal instillation to establish a stable sialin knockdown model.

Results: Submandibular gland tissue structure could completely restore 28 days after duct deligation, when the duct had been ligated for 7 days. The expression of sialin, polysialic acid, and polysialyltransferase IV was significantly increased on day 0 after duct deligation, and it returned to the level of the control group at day 28. Moreover, sialin knockdown could weakened gland regeneration by reducing polysialic acid synthesis. Supplementing drinking water with polysialic acid precursors (ManNAc) in drinking water could partially rescue submandibular gland regeneration in sialin knockdown rats.

Conclusion: These data indicated that sialin was vital for submandibular gland regeneration which mediated the process of gland regeneration by affecting the polysialic acid synthesis.
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http://dx.doi.org/10.1111/odi.14256DOI Listing
May 2022

Recycling of zinc-carbon batteries into MnO/ZnO/C: a smart strategy to fabricate sustainable cathode for rechargeable zinc ion batteries.

ChemSusChem 2022 May 19. Epub 2022 May 19.

Henan Normal University, School of Mateirals Science and Engineering, CHINA.

Acidic zinc-carbon dry batteries have been widely used in life because of their low cost. However, a great quantity of used batteries is discarded as refuse, which not only causes the waste of resources but also leads to environmental contamination. To reuse the spent batteries on a large scale, here we propose a simple, effective and sustainable strategy to turn them into MnO/ZnO/C composites. Only after a conventional leaching treatment followed by pyrolysis, the rust cathode materials can be reduced to MnO/ZnO/C. When serving as a rechargeable zinc-ion battery cathode, such electrode provides a maximum reversible capacity (~ 362 mAh g -1 MnO ) and prominent rate capability ( 191 mAh g -1 MnO at a high current rate of 1.20 A g -1 ). Furthermore, it is found that ZnO gradually dissolves in the electrolyte with the increase of discharge cycles, replenishing the Zn 2+ content in the electrolyte and thus ensuring excellent cycle stability (98.02% after 500 cycles), while the device also possesses remarkable energy density (336.37 Wh kg -1 ), low self-discharge rate, and can efficiently power a LED panel. Our paradigm work offers an economical, scalable and facile route to convert the useless zinc-carbon batteries wastes into useful materials for aqueous rechargeable zinc ion batteries.
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http://dx.doi.org/10.1002/cssc.202200720DOI Listing
May 2022

Corrigendum: Plasma Exosomal Proteomic Pattern of Epstein-Barr Virus-Associated Hemophagocytic Lymphohistiocytosis.

Front Microbiol 2022 3;13:919026. Epub 2022 May 3.

Department of Hematology, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.

[This corrects the article DOI: 10.3389/fmicb.2022.821311.].
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http://dx.doi.org/10.3389/fmicb.2022.919026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111955PMC
May 2022

Research on Underwater Wet Laser Self-Fusion Welding Process and Analysis of Microstructure and Properties of TC4 Titanium Alloy Weld.

Materials (Basel) 2022 May 8;15(9). Epub 2022 May 8.

National Engineering Research Center for Remanufacturing, Army Academy of Armored Forces, Beijing 100072, China.

In order to explore the feasibility of underwater wet laser welding of the TC4 titanium alloy, research on the underwater laser self-fusion welding process was carried out. The weld structure and mechanical properties in both the air environment and the underwater environment were compared and analyzed. The results show that increasing the laser power and reducing the welding speed are beneficial to obtain a larger water depth threshold. Off-focus amount has little effect on water depth threshold; when the laser power is 3000 W and the welding speed is 5 mm/s, and the water depth exceeds 7 mm, a continuous weld cannot be formed. Compared with welding in the air, underwater welding has narrower weld width, smaller heat affected zone and finer crystal grains. The weld structure is mainly composed of α' martensite and secondary acicular α' phase, it is distributed in a net basket shape and the grain size at the top of the weld is finer. The hardness of the weld center is above 600 HV0.1, and the residual stress of the underwater welding weld is approximately symmetrically distributed. There is a large tensile stress along the welding direction at the weld, reaching 458 MPa. The larger residual tensile stress leads to the decrease of weld tensile strength, the tensile strength and elongation of the middle sample are only 52% and 77% of the base metal. Furthermore, the fracture mode is typical brittle fracture.
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http://dx.doi.org/10.3390/ma15093380DOI Listing
May 2022

Characteristics of childhood-onset systemic lupus erythematosus in pregnancy and its association with pregnancy outcomes: a retrospective comparative cohort study.

Reprod Biol Endocrinol 2022 May 19;20(1):78. Epub 2022 May 19.

Department of Epidemiology, School of Public Health, Southern Medical University, No.1846, North of Guangzhou Avenue, Guangzhou, 510515, Guangdong, China.

Background: Disease situations are more aggressive in patients with childhood-onset systemic lupus erythematosus (cSLE) than in those with adult-onset SLE (aSLE). However, information on pregnant women with cSLE and its association with pregnancy outcomes is limited. This study aimed to compare pregnancies in patients with cSLE vs. aSLE, and further analyse the characteristics of cSLE in pregnant women and explore its association with adverse pregnancy outcomes.

Methods: Altogether, data of 167 pregnancies from 150 women, including 22 pregnancies with cSLE and 145 pregnancies with aSLE, were retrospectively analysed. Characteristics and disease activity were compared between the cSLE and aSLE groups during pregnancy. Associations between cSLE and the risk of active SLE (SLEPDAI > 4), active lupus nephritis (LN), and adverse pregnancy outcomes were analysed using logistic regression.

Results: The cSLE group had a higher incidence of active SLE (12/22 vs. 30/145, P = 0.001) and active LN (11/22 vs. 26/145, P = 0.001) than the aSLE group. In the multivariable analysis, cSLE was a risk factor for active SLE and active LN during pregnancy, with ORs of 4.742 (95%CI 1.678-13.405, P = 0.003) and 4.652 (95%CI 1.630-13.279, P = 0.004), respectively. No significant association between cSLE and the risk of composite adverse gestational outcomes was identified after sequentially adjusting pre-pregnancy characteristics and pregnancy factors (P > 0.05).

Conclusion: Disease activity of women with cSLE in pregnancy was more aggressive than that of women with aSLE, which was similar to the characteristics of non-pregnant women with SLE. cSLE might have indirect effects on the risk of adverse pregnancy outcomes through LN and active disease. Therefore, closely monitoring patients with cSLE during pregnancy is crucial.
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http://dx.doi.org/10.1186/s12958-022-00954-xDOI Listing
May 2022

Bulk and single-cell transcriptome profiling reveal necroptosis-based molecular classification, tumor microenvironment infiltration characterization, and prognosis prediction in colorectal cancer.

J Transl Med 2022 May 19;20(1):235. Epub 2022 May 19.

Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: Necroptosis is a new form of programmed cell death that is associated with cancer initiation, progression, immunity, and chemoresistance. However, the roles of necroptosis-related genes (NRGs) in colorectal cancer (CRC) have not been explored comprehensively.

Methods: In this study, we obtained NRGs and performed consensus molecular subtyping by "ConsensusClusterPlus" to determine necroptosis-related subtypes in CRC bulk transcriptomic data. The ssGSEA and CIBERSORT algorithms were used to evaluate the relative infiltration levels of different cell types in the tumor microenvironment (TME). Single-cell transcriptomic analysis was performed to confirm classification related to NRGs. NRG_score was developed to predict patients' survival outcomes with low-throughput validation in a patients' cohort from Fudan University Shanghai Cancer Center.

Results: We identified three distinct necroptosis-related classifications (NRCs) with discrepant clinical outcomes and biological functions. Characterization of TME revealed that there were two stable necroptosis-related phenotypes in CRC: a phenotype characterized by few TME cells infiltration but with EMT/TGF-pathways activation, and another phenotype recognized as immune-excluded. NRG_score for predicting survival outcomes was established and its predictive capability was verified. In addition, we found NRCs and NRG_score could be used for patient or drug selection when considering immunotherapy and chemotherapy.

Conclusions: Based on comprehensive analysis, we revealed the potential roles of NRGs in the TME, and their correlations with clinicopathological parameters and patients' prognosis in CRC. These findings could enhance our understanding of the biological functions of necroptosis, which thus may aid in prognosis prediction, drug selection, and therapeutics development.
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http://dx.doi.org/10.1186/s12967-022-03431-6DOI Listing
May 2022

Rhein activated Fas-induced apoptosis pathway causing cardiotoxicity in vitro and in vivo.

Toxicol Lett 2022 May 16. Epub 2022 May 16.

State Key Laboratory of Traditional Chinese Medicine Resources in Southwest China, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. Electronic address:

Rhein, one of the main active components of rhubarb (Dahuang) and Polygonum multiflorum (Heshouwu), has a wide range of effective pharmacological effects. Recently, increasing studies have focused on its potential hepatorenal toxicity, but the cardiotoxicity is unknown. In this study, we found that the IC of rhein to H9c2 cells at 24h and 48h were 94.5 and 45.9μmol/L, respectively, with positive correlation of dose-toxicity and time-toxicity. After the treatment of rhein (106, 124 and 132μmol/L), the number of H9c2 cells decreased significantly, and the morphology of H9c2 cells showed atrophy, round shape and wall detachment. Moreover, the proportion of apoptotic cells in H9c2 cells treated with rhein was significantly increased in a dose-dependent manner. And rhein induced S phase arrest of H9c2 cells and inhibited cell proliferation. Rhein up-regulated ROS, LDH levels and low MMP but down-regulated SOD content in H9c2 cells. Additionally, the results showed that the cardiac function LVEF and LVFS of rhein high-medium-low dose groups (350, 175, 87.5mg/kg) were significantly reduced. And the contents of Ca, cTnT, CK and LDH in serum of KM mice were significantly up-regulated by rhein. Furthermore, western blot results suggested that rhein the above effects via promoting Fas-induced apoptosis pathway in vitro and in vivo. In general, rhein may cause cardiotoxicity via Fas-induced apoptosis pathway in vivo and in vitro, which provides reference for the safe use of medicinal plant containing rhein and its preparations.
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http://dx.doi.org/10.1016/j.toxlet.2022.04.006DOI Listing
May 2022

Cardiac Angiosarcomas: Risk of Brain Metastasis and Hemorrhage Warrants Frequent Surveillance Imaging and Early Intervention.

Am J Clin Oncol 2022 Jun 26;45(6):258-263. Epub 2022 Apr 26.

Sarcoma Medical Oncology.

Purpose: We evaluated a cohort of patients with cardiac angiosarcomas (CA) who developed brain metastases (BM) to define outcomes and intracranial hemorrhage (IH) risk.

Methods: We reviewed 26 consecutive patients with BM treated between 1988 and 2020 identified from a departmental CA (n=103) database. Causes of death were recorded, and a terminal hemorrhage (TH) was defined as an IH that caused death or prompted a transfer to hospice.

Results: The prevalence of BM was 25% (n=26/103). A total of 23 patients (88%) had IH, including 21 (81%) at initial BM diagnosis, of which 18 (86%) required hospitalization. The median platelet count at the time of IH was 235k (interquartile range, 108 to 338k).Nearly all patients died of disease (n=23, 88%) and most patients died from TH (n=13, 57%). TH occurred at BM presentation in 6 (46%) patients, whereas 3 (23%) had TH from known but untreated lesions, 2 (15%) had continued uncontrolled IH during radiation therapy, and 2 (15%) from new BM. Platelet count <50k was not associated with TH (P=0.25).Subsequent IH occurred in 9 patients (35%), and importantly, no patients who completed radiation therapy (n=10) for BM died from TH.

Conclusion: Patients with CA frequently develop BM, and the risk of IH is high, resulting in an alarming rate of TH despite normal platelet counts. Therefore, early diagnosis and intervention are warranted. We recommend surveillance brain imaging, and importantly, once BM is detected, prompt local therapy is warranted to try and mitigate the risk of TH.
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http://dx.doi.org/10.1097/COC.0000000000000913DOI Listing
June 2022

Time-Atom Economical Regio-and Chemoselective Radical Cyclization of Unactivated 1,6-Enynes under Metal- and Oxidant-free Conditions.

Chemistry 2022 May 19. Epub 2022 May 19.

Kaohsiung Medical University, Department of Medicinal and Applied Chemistry, No. 100, Shiquan 1st Road, Sanmin District, Kaohsiung City, 807, 807, Kaohsiung City, TAIWAN.

We developed time-atom economic regio- and chemoselective sulfonyl radical triggered 5-exo-dig cyclization of unactivated 1,6-enynes with sulfonyl halides under metal, additive-free reaction conditions to achieve highly substituted five-membered heterocyclic compounds. This transformation creates three new bonds, such as C-SO2 , C-C, and active C-I/Br bonds. Importantly, one-pot protocols produce desired products directly from sodium sulfinates and have an additional advantage such as minimising chemical waste, saving time, and simplifying practical aspects compared to existing protocols.
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http://dx.doi.org/10.1002/chem.202200742DOI Listing
May 2022

[Application of two different comfort techniques in extraction of impacted teeth among 60 patients with hypertension].

Shanghai Kou Qiang Yi Xue 2022 Feb;31(1):109-112

Department of Anesthesiology, Shanghai Stomatological Hospital, Fudan University. Shanghai 200001, China. E-mail:

Purpose: To compare the effect of two comfort techniques of nitrous oxide/oxygen inhalation and dexmedetomidine combined with flurbiprofen axetil sedation and analgesia in impacted teeth extraction among patients with hypertension.

Methods: According to the inclusion and exclusion criteria, sixty patients undergoing impacted teeth extraction with hypertension were randomly divided into 2 groups: X group( nitrous oxide/oxygen inhalation, n=30) and Y group(dexmedetomidine combined with flurbiprofen axetil assisted sedation and analgesia, n=30). The following parameters at T0(preoperative), T1(local anesthesia), T2 (surgical operation), and T3 (5 minutes after surgery) were recorded: mean arterial pressure(MAP), heart rate (HR), Ramsay sedation score, VAS pain score. SPSS 20.0 software package was used for statistical analysis.

Results: The parameters at each time point between the two groups were not significantly different (P>0.05). Mood and hemodynamics at T1, T2 and T3 in each group were more stable compared with those at T0(P<0.05).

Conclusions: The two comfort techniques can stabilize the patients' mood, keep the patients' hemodynamics stable, and are safe and effective in extraction of impacted teeth among patients with hypertension.
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February 2022

Role of Ions in Hydrogels with an Ionic Seebeck Coefficient of 52.9 mV K.

J Phys Chem Lett 2022 May 19:4621-4627. Epub 2022 May 19.

MOE Key Laboratory of Low-grade Energy Utilization Technologies and Systems, CQU-NUS Renewable Energy Materials & Devices Joint Laboratory, School of Energy & Power Engineering, Chongqing University, Chongqing 400044, China.

Ionic thermoelectric (i-TE) material with mobile ions as charge carriers has the potential to generate large thermal voltages at low operating temperatures. This study highlights the role of ions in i-TE hydrogels employing a poly(vinyl alcohol) (PVA) polymer matrix and a number of ion providers, e.g., KOH, KNO, KCl, KBr, NaI, KI, and CsI. The relationship between the intrinsic physical parameters of the ion and the thermoelectric performance is established, indicating the ability to influence the hydrogen bond by the ion is a crucial factor. Among these i-TE hydrogels, the PVA/CsI hydrogel exhibits the largest ionic Seebeck coefficient, reaching 52.9 mV K, which is the largest of all i-TE materials reported to date. In addition, our work demonstrates the influence of ions on polymer configuration and provides an avenue for ion selection in the Soret effect in ionic thermoelectrics.
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http://dx.doi.org/10.1021/acs.jpclett.2c00845DOI Listing
May 2022

Effect of Warming Yang, Tonifying Kidney, and Removing Arthralgia Therapy on Cold-Dampness Arthralgia Type Ankylosing Spondylitis and Its Influence on the Levels of Humoral Factor in Human Serum.

Authors:
Jing Li

Biomed Res Int 2022 9;2022:8348272. Epub 2022 May 9.

Affiliated Hospital of Hubei University of Traditional Chinese Medicine, Hubei Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430060, China.

Objective: This study is aimed at exploring the effect of warming Yang, tonifying kidney, and removing arthralgia therapy in the treatment of cold-dampness arthralgia type ankylosing spondylitis (AS) and the effects on the levels of humoral factor in human serum A total of 72 patients with cold-dampness arthralgia type AS treated in our hospital from May 2020 to June 2021 were selected and divided into the observation group ( = 36) and control group ( = 36) according to the random number table method. The clinical efficacy of the two groups was observed. The traditional Chinese medicine (TCM) syndrome scores and clinical signs of the two groups were compared, and the pain situation of the two groups was evaluated by visual analog scale (VAS). Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) were used to evaluate the spinal function and activity of the two groups, and the levels of CXC-type chemokine ligand 16 (CXCL16), Dickkopf-1(DKK-1), interleukin-17 (IL-17), tumor necrosis factor- (TNF-), sclerostin (SOST) ,and bone morphogenetic protein 2(BMP-2) in serum of the two groups were measured.

Results: The total effective rate of the observation group (91.67%) was significantly higher than that of the control group (66.67%) ( < 0.05). After treatment, the degree of improvement in TCM syndrome score, clinical signs, VAS score, BASDAI, BASFI, and the levels of CXCL16, TNF-, IL-17, DKK-1, SOST, and BMP-2 in the observation group were significantly higher than that in the control group (all < 0.05) Warming Yang, tonifying kidney, and removing arthralgia therapy had a good effect on the treatment of cold-dampness arthralgia type AS, and it could effectively improve the clinical symptoms and signs, relieve pain, improve spinal motion, and relieve inflammation of patients.
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http://dx.doi.org/10.1155/2022/8348272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110190PMC
May 2022

Serum Atrial Natriuretic Peptide, Promoter Methylation, and Cardiovascular Disease: A 10-year Follow-Up Study in Chinese Adults.

Glob Heart 2022 7;17(1):27. Epub 2022 Apr 7.

Department of Epidemiology, School of Public Health, Medical College of Soochow University, Suzhou, China.

Background: Atrial natriuretic peptide (ANP) has been associated with cardiovascular disease (CVD) and related risk factors, but the clinical application is limited and the underlying mechanisms are not very clear. Here, we aimed to examine whether proANP and its coding gene methylation were associated with CVD in the Chinese population.

Methods: Serum proANP and peripheral blood DNA methylation of natriuretic peptide A gene () promoter was quantified at baseline for 2,498 community members (mean aged 53 years, 38% men) in the Gusu cohort. CVD events were obtained during 10 years of follow-up. A competing-risks survival regression model was applied to examine the prospective associations of proANP and promoter methylation with incident CVD.

Results: During follow-up, 210 participants developed CVD events, 50 participants died from non-cardiovascular causes, and 214 participants were lost. Per 1-nmol/L increment of serum proANP was associated with a 22% (HR = 1.22, 95%CI: 1.03-1.44, = 0.025) higher risk of CVD during follow-up. Of the 9 CpG sites assayed, per 2-fold increment of DNA methylation at CpG3 (located at Chr1:11908299) was significantly associated with a half lower risk of CVD (HR = 0.50, 95%CI: 0.30-0.82, = 0.006). The gene-based analysis found that DNA methylation of the 9 CpGs at promoter as a whole was significantly associated with incident CVD ( < 0.05).

Conclusions: Increased proANP and hypomethylation at promoter at baseline predicted an increased future risk of CVD in Chinese adults. Aberrant DNA methylation of the gene may participate in the mechanisms of CVD.
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http://dx.doi.org/10.5334/gh.1116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8992767PMC
May 2022

Efficient removal of organic compounds in eutrophic water via a synergy of cyanobacterial extracellular polymeric substances and permanganate.

Environ Sci Pollut Res Int 2022 May 19. Epub 2022 May 19.

School of Environment, Nanjing Normal University, Nanjing, 210023, China.

This study provides a new thinking for the efficient utilization of permanganate (Mn (VII)) in eutrophic water treatment. Eutrophic water contained a large amount of extracellular polymeric substances (EPS) with reduction and chelation; this study used phenol as typical organic matter and cyanobacteria EPS as a representative EPS to explore the mechanism by which EPS influences the oxidation of phenol by Mn(VII) at pH 5.0-9.0. The results showed that under the condition of pH 5.0-7.0, adding 0.2-10 mg/L EPS to the Mn(VII) system could effectively improve the oxidation efficiency of Mn(VII) for phenol. EPS promoted the continuous formation and stability of in situ EPS-MnO colloids and significantly enhanced the oxidation of Mn(VII). EPS also combined with phenol and increased the electron cloud density to promote the oxidation of phenol by Mn(VII).
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http://dx.doi.org/10.1007/s11356-022-20827-5DOI Listing
May 2022

A non-invasive model based on the virtual portal pressure gradient to predict the first variceal hemorrhage in cirrhotic patients.

Hepatol Int 2022 May 18. Epub 2022 May 18.

Department of Gastroenterology and Hepatology, Tongji Hospital, School of Medicine, Tongji University, 389 Xincun Road, Shanghai, 200092, China.

Background And Aims: This study aimed to establish a non-invasive model based on the virtual portal pressure gradient (vPPG) to predict the first variceal hemorrhage (VH) in patients with cirrhosis.

Methods: This single-center study prospectively enrolled cirrhotic patients as the training and validation cohorts during different time periods. The PPG-detection software (PPGS 1.0) was used to perform vPPG calculation, which involves 2 steps including three-dimensional (3D) reconstruction of portal vein tree and subsequent application of computational fluid dynamics. All patients were given standard primary prophylaxis against VH and followed up for 2 years. Data from the training cohort were assessed using univariate and multivariate Cox regression and Kaplan-Meier analyses, by which a nomogram with its dynamic form was developed to estimate the probability of VH.

Results: In the training cohort (n = 128), 37 (28.9%) experienced VH during 2-year follow-up. Four variables including vPPG ≥ 10.5 mmHg (p < 0.001), PLT < 56 × 10/L (p = 0.048), albumin < 32 g/L (p < 0.001) and INR ≥ 1.2 (p = 0.022) were identified as independent risk factors of VH, among which vPPG showed the best diagnostic performance (AUC 0.875). Subsequently, these predictors were incorporated into the nomogram, of which C-indexes were 0.891 and 0.926 for the training and validation cohorts, respectively. Calibration curves demonstrated a great calibration ability of the model. At the threshold probabilities of 0.1-0.6 (1 year) and 0.1-1.0 (2 years), this nomogram could offer more net benefits in decision curve analysis.

Conclusions: The vPPG-based nomogram could be used for risk stratification of the first VH in patients with cirrhosis.
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http://dx.doi.org/10.1007/s12072-022-10344-5DOI Listing
May 2022

Interfacial electronic properties of metal/CsSnBr3 heterojunctions.

Nanotechnology 2022 May 18. Epub 2022 May 18.

Nanjing University of Science and Technology, No.200 Xiao Lingwei Street, Xuan Wu District, Nan Jing City, Jiang Su, Nanjing, 210094, CHINA.

All-inorganic lead-free perovskite CsSnBr3, has been proved good stability and optoelectronic properties in theory and experiment. However, the interfacial electronic properties of metal/CsSnBr3 are still unclear in electronic devices. Herein, we systematically investigate the interfacial properties of metal electrodes (Al, Ag and Au) and CsSnBr3 with different atomic terminals (SnBr2-T and CsBr-T) through the first-principles calculation. SnBr2-T and CsBr-T have various contact types and Schottky barriers due to their different interaction strengths with metals. In particular, the moderate interlayer coupling strength with Al leads to the ultra-low Schottky barrier and tunneling barrier, which makes Al possess the best contact performance among the studied metals. Furthermore, the external electric field can be effective in regulating the Schottky barrier and realizing the Ohmic contact. These findings provide useful guidance for the design of perovskite-based nanoelectronic devices with high performance.
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http://dx.doi.org/10.1088/1361-6528/ac70e6DOI Listing
May 2022

lncRNA GAS5 promotes pyroptosis in COPD by functioning as a ceRNA to regulate the miR‑223‑3p/NLRP3 axis.

Mol Med Rep 2022 Jul 18;26(1). Epub 2022 May 18.

Department of General Practice, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China.

Chronic obstructive pulmonary disease (COPD) is characterized by irreversible and progressive airflow limitation and encompasses a spectrum of diseases, including chronic obstructive bronchitis and emphysema. Pyroptosis is a unique form of inflammatory cell death mediated by the activation of caspase‑1 and inflammasomes. The long non‑coding RNA (lncRNA) growth arrest‑specific 5 (GAS5) is a well‑documented tumor suppressor, which is associated with cell proliferation and death in various diseases. The aim of the present study was to evaluate whether lncRNA GAS5 is associated with the pyroptosis in COPD. To create a COPD cell model, MRC‑5 cells were treated with 10 µg/ml lipopolysaccharide (LPS) for 48 h. Then the level of pro‑caspase 1, caspase 1, IL‑1β, IL‑18, NLRP3 and cleaved gasdermin D (GSDMD) was examined by western blotting. GAS5 mRNA level was detected by qualitative PCR following LPS treatment in MRC‑5 cells. Subsequently, IL‑2, IL‑6, IL‑10 and TNF‑α in MRC‑5 cells was measured by ELISA. Then the proliferation ability of MRC‑5 cells was detected by CCK‑8. Cell death was detected by TUNEL assay. LDH release was measured using an LDH Cytotoxicity Assay kit. The Magna RIP kit was used to validate the interaction between GAS5 and miR‑223‑3p. The present study revealed that increased expression levels of caspase‑1, IL‑1β, IL‑18 and cleaved GSDMD were observed in LPS‑treated MRC‑5 cells, indicating that pyroptosis is involved in COPD progression. Additionally, LPS induced the increase in GAS5 mRNA expression levels and the release of inflammatory factors (IL‑2, IL‑6, IL‑10 and TNF‑α), suggesting that GAS5 is implicated in pyroptosis in COPD. Furthermore, upregulation of GAS5 promoted cell death and inhibited proliferation in the MRC‑5 cell line. Additionally, increased GAS5 expression significantly promoted the production of caspase‑1, IL‑1β, IL‑18, cleaved GSDMD and NLR pyrin domain containing protein 3 (NLRP3). A dual‑luciferase assay demonstrated that GAS5 could directly bind to microRNA‑223‑3p (miR‑223‑3p), and NLRP3 is a direct target of miR‑223‑3p. Furthermore, GAS5 reduced the expression levels of miR‑223‑3p, while it increased the expression levels of NLRP3. The present study concluded that lncRNA GAS5 promoted pyroptosis in COPD by targeting the miR‑223‑3p/NLRP3 axis, implying that GAS5 could be a potential target for COPD.
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http://dx.doi.org/10.3892/mmr.2022.12735DOI Listing
July 2022

An immune gene signature to predict prognosis and immunotherapeutic response in lung adenocarcinoma.

Sci Rep 2022 May 17;12(1):8230. Epub 2022 May 17.

School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, Fujian, China.

Lung adenocarcinoma is one of the most common malignant tumors worldwide. The purpose of this study was to construct a stable immune gene signature for prediction of prognosis (IGSPP) and response to immune checkpoint inhibitors (ICIs) therapy in LUAD patients. Five genes were screened by weighted gene coexpression network analysis, Cox regression and LASSO regression analyses and were used to construct the IGSPP. The survival rate of the IGSPP low-risk group was higher than that of the IGSPP high-risk group. Multivariate Cox regression analysis showed that IGSPP could be used as an independent prognostic factor for the overall survival of LUAD patients. IGSPP genes were enriched in cell cycle pathways. IGSPP gene mutation rates were higher in the high-risk group. CD4 memory-activated T cells, M0 and M1 macrophages had higher infiltration abundance in the high-risk group, which was associated with poor overall survival. In contrast, the abundance of resting CD4 memory T cells, monocytes, resting dendritic cells and resting mast cells associated with a better prognosis was higher in the low-risk group. TIDE scores and the expressions of different immune checkpoints showed that patients in the high-risk IGSPP group benefited more from ICIs treatment. In short, an IGSPP of LUAD was constructed and characterized. It could be used to predict the prognosis and benefits of ICIs treatment in LUAD patients.
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http://dx.doi.org/10.1038/s41598-022-12301-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114138PMC
May 2022

Acupuncture for chronic sciatica: protocol for a multicenter randomised controlled trial.

BMJ Open 2022 May 17;12(5):e054566. Epub 2022 May 17.

International Acupuncture and Moxibustion Innovation Institute, School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China

Background: Chronic Sciatica is a disabling condition causing considerable medical, social and financial implications. Currently, there is no recognised long-term effective treatment to alleviate sciatica. Acupuncture has been widely used for treating chronic pains with persistent analgesic effects. We aim to evaluate the efficacy and safety of acupuncture for chronic sciatica with follow-up in 52 weeks.

Methods And Analysis: This is a multicenter randomised sham-controlled trial. A total of 216 patients with chronic sciatica will be enrolled and randomly assigned to the acupuncture or sham acupuncture group. There will be 10 treatment sessions applied in 4 weeks with frequency decreased over time. Patients will complete follow-ups during 52 weeks. The primary outcomes are changes in leg pain intensity and disability from baseline to week 4. Secondary outcomes include back pain intensity, frequency and bothersomeness, quality of life, and global perceived effect. Adverse events will be recorded in detail.

Ethics And Dissemination: Ethical approval of this trial was granted from the ethics committee of Beijing University of Chinese Medicine and all study centres (No. 2020BZYLL0803). Written informed consent will be obtained from enrolled patients. Trial results will be disseminated in peer-reviewed publications.

Trial Registration Number: ChiCTR2100044585 (Chinese Clinical Trial Registry, http://www.chictr.org.cn, registered on 24 March 2021); preresults.
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http://dx.doi.org/10.1136/bmjopen-2021-054566DOI Listing
May 2022

Effectiveness of a clinical decision support system for hypertension management in primary care: study protocol for a pragmatic cluster-randomized controlled trial.

Trials 2022 May 16;23(1):412. Epub 2022 May 16.

National Clinical Research Center for Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Fuwai Hospital, National Center for Cardiovascular Diseases, 167 Beilishi Road, Beijing, 100037, China.

Background: Clinical decision support systems (CDSS) are low-cost, scalable tools with the potential to improve guideline-based antihypertensive treatment in primary care, but their effectiveness needs to be tested, especially in low- and middle-income countries such as China.

Methods: The Learning Implementation of Guideline-based decision support system for Hypertension Treatment (LIGHT) trial is a pragmatic, four-stage, cluster-randomized trial conducted in 94 primary care sites in China. For each city-based stage, sites are randomly assigned to either implementation of the CDSS for hypertension management (which guides doctors' treatment recommendations based on measured blood pressure and patient characteristics), or usual care. Patients are enrolled during the first 3 months after site randomization and followed for 9 months. The primary outcome is the proportion of hypertension management visits at which guideline-based treatment is provided. In a nested trial conducted within the CDSS, with the patient as the unit of randomization, the LIGHT-ACD trial, patients are randomized to receive different initial mono- or dual-antihypertensive therapy. The primary outcome of the LIGHT-ACD trial is the changes in blood pressure.

Discussion: The LIGHT trial will provide evidence on the effectiveness of a CDSS for improving guideline adherence for hypertension management in primary care in China. The nested trial, the LIGHT-ACD trial, will provide data on the effect of different initial antihypertensive regimens for blood pressure management in this setting.

Trial Registration: ClinicalTrials.gov, identifier: LIGHT (NCT03636334) and LIGHT-ACD (NCT03587103). Registered on 3 July 2018.
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http://dx.doi.org/10.1186/s13063-022-06374-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109449PMC
May 2022

A species diversity dataset of beetles by three passive acquisition methods in Tei Tong Tsai (Hong Kong).

Sci Data 2022 May 16;9(1):210. Epub 2022 May 16.

Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Box 92, Beichen West Road, Chaoyang District, Beijing, 100101, China.

We based the dataset in this paper on the beetle collection from the sample site of Tei Tong Tsai (Hong Kong) from 1 May to 28 May 2019, a period of high insect diversity. A total of 16,270 beetles (photographed in 318 images) from 478 species belonging to 39 families were collected. The dataset consists of the following components: The original photo of the whole sample obtained at each site with each collection method, the morphological species identification chart, a statistical table describing the species and numbers of beetles collected on different dates at different sites using three passive acquisition methods, and a statistical table describing the longitude, latitude, and altitude information of each sampling point. We aimed to provide a database for the evaluation of beetle species diversity in Hong Kong and a paradigm for the effectiveness of passive acquisition in the beetle collection through the three representative methods, thus laying a foundation for biodiversity research.
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http://dx.doi.org/10.1038/s41597-022-01310-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110429PMC
May 2022

Protein-Crowned Micelles for Targeted and Synergistic Tumor-Associated Macrophage Reprogramming to Enhance Cancer Treatment.

Nano Lett 2022 May 16. Epub 2022 May 16.

Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China.

Tumor-associated macrophages (TAMs) are a promising therapeutic target for cancers, but achieving multitarget therapy of TAMs is still challenging. Here, we develop a protein-crowned micelle system for targeted and synergistic TAM reprogramming to enhance cancer treatment. The doxorubicin-loaded micelles with a hemoglobin crown (Hb-DOXM) can bind with endogenous plasma haptoglobin to realize specific M2-type TAM targeting. Under the tumor hypoxic and acidic environments, Hb-DOXM can responsively release O and DOX to reduce the recruitment of TAMs by hypoxia remission and release DOX to kill M2-type TAMs and cancer cells. To reprogram TAMs adequately, the TAM-modulating drug celecoxib is further encapsulated ([email protected]) to repolarize M2-type TAMs. The targeted and synergistic TAM reprogramming by [email protected] can remodel the tumor microenvironment (TME) to an immunostimulatory microenvironment and augment the antitumor effect of cytotoxic T lymphocyte, thus strongly enhancing the DOX-based chemotherapy. The protein-crowned micelle strategy presents a targeted and synergistic TAM therapy tool for enhanced cancer treatment.
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http://dx.doi.org/10.1021/acs.nanolett.2c00901DOI Listing
May 2022

Integrating abdominal plain radiographs and clinical data for neonatal necrotizing enterocolitis using hypergraph-based multi-constraint combined non-negative matrix factorization to construct a diagnostic model.

Bosn J Basic Med Sci 2022 May 15. Epub 2022 May 15.

Department of Data and Information, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China; Sino-Finland Joint AI Laboratory for Child Health of Zhejiang Province, Hangzhou, China; National Clinical Research Center for Child Health, Hangzhou, China.

Neonatal necrotizing enterocolitis is a severe neonatal intestinal disease. Timely identification of surgical indications is essential for newborns in order to seek the best time for treatment and improve prognosis. This paper attempts to establish an algorithm model based on multimodal clinical data to determine the features of surgical indications and construct an auxiliary diagnosis model. The proposed algorithm adds hypergraph constraints on the two modal data based on Joint Nonnegative Matrix Factorization (JNMF), aiming to mine the higher-order correlations of the two data features. In addition, the adjacency matrix of the two kinds of data is used as a network regularization constraint to prevent overfitting. Orthogonal and L1-norm regulations were introduced to avoid feature redundancy and perform feature selection, respectively, and confirmed 14 clinical features. Finally, we used three classifiers, random forest, support vector machine, and logistic regression, to perform binary classification of patients requiring surgery. The results show that when the features selected by the proposed algorithm model are classified by random forest, the area under the ROC curve is 0.8, which has high prediction accuracy.
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http://dx.doi.org/10.17305/bjbms.2022.7046DOI Listing
May 2022

2-Naphthalenemethanol participates in metabolic activation of 2-methylnaphthalene.

Xenobiotica 2022 May 16:1-34. Epub 2022 May 16.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Guizhou Medical University, Guiyang, 550004, Guizhou, P R China.

1.2-Methylnaphthalene (2-MN) is an environmental pollutant. Studies have shown that 2-MN is teratogenic, carcinogenic and cytotoxic. However, the mechanisms of 2-MN induced toxicities remain unclear.2.The present study aimed to characterize reactive metabolites of 2-MN, to define the metabolic pathway, and to determine the enzymes participating in the metabolic activation.3.A hydroxylation metabolite of 2-MN, 2-naphthalenemethanol (2-NM), was observed in 2-MN-containing mouse liver microsomes.4.A glutathione (GSH) conjugate was detected in mouse S9 incubations fortified with 2-MN and GSH. A GSH conjugate and an NAC conjugate were found in mouse liver and urine respectively in animals given 2-MN. Hepatic protein covalent binding derived from 2-NM was observed in animals administered 2-MN.5.Cytochrome P450 enzymes and sulfotransferases participated in the metabolic activation of 2-MN.
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http://dx.doi.org/10.1080/00498254.2022.2079022DOI Listing
May 2022

The Gene From Pepper ( L.) Confers Chilling Stress Tolerance in Transgenic Plants.

Front Plant Sci 2022 29;13:884990. Epub 2022 Apr 29.

College of Horticulture, Gansu Agriculture University, Lanzhou, China.

The gene encodes an enzyme that is essential for chlorophyll biosynthesis and is involved in many other physiological processes in plants. In this study, the gene was cloned from pepper and sequenced. Multiple sequence alignment and phylogenetic analysis of ALAD proteins from nine plant species showed that is highly conserved, and that CaALAD shows the highest homology with the ALAD protein from eggplant. Subcellular localization indicated that the CaALAD protein is mainly localized to the chloroplasts. After transferring into the genome, cold tolerance of the transgenic lines improved. Overexpression of increased the relative transcription of the , , and genes in transgenic plants exposed to low temperature (4°C) stress, and the contents of reactive oxygen species decreased due to increased activities of superoxide dismutase, peroxidase, and catalase. Moreover, chlorophyll biosynthesis, as determined by the contents of porphobilinogen, protoporphyrin IX, Mg-protoporphyrin IX, prochlorophyllate, and chlorophyll in the transgenic plants, increased in response to low temperature stress. In addition, the transgenic lines were more sensitive to exogenous ALA and NaHS, and the HS content of transgenic line plants increased more rapidly than in the wild-type, suggesting that may respond to low temperatures by influencing the content of HS, a signaling molecule. Our study gives a preliminary indication of the function of and will provide a theoretical basis for future molecular breeding of cold tolerance in pepper.
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http://dx.doi.org/10.3389/fpls.2022.884990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100946PMC
April 2022

Research Progress and Trends in Metabolomics of Fruit Trees.

Front Plant Sci 2022 29;13:881856. Epub 2022 Apr 29.

Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Metabolomics is an indispensable part of modern systems biotechnology, applied in the diseases' diagnosis, pharmacological mechanism, and quality monitoring of crops, vegetables, fruits, etc. Metabolomics of fruit trees has developed rapidly in recent years, and many important research results have been achieved in combination with transcriptomics, genomics, proteomics, quantitative trait locus (QTL), and genome-wide association study (GWAS). These research results mainly focus on the mechanism of fruit quality formation, metabolite markers of special quality or physiological period, the mechanism of fruit tree's response to biotic/abiotic stress and environment, and the genetics mechanism of fruit trait. According to different experimental purposes, different metabolomic strategies could be selected, such as targeted metabolomics, non-targeted metabolomics, pseudo-targeted metabolomics, and widely targeted metabolomics. This article presents metabolomics strategies, key techniques in metabolomics, main applications in fruit trees, and prospects for the future. With the improvement of instruments, analysis platforms, and metabolite databases and decrease in the cost of the experiment, metabolomics will prompt the fruit tree research to achieve more breakthrough results.
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http://dx.doi.org/10.3389/fpls.2022.881856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106391PMC
April 2022
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