Publications by authors named "Jing Jiang"

846 Publications

Development and validation of a new prognostic score for hepatitis B virus-related acute-on-chronic liver failure.

J Hepatol 2021 Jun 3. Epub 2021 Jun 3.

Institute of Pharmaceutical Biotechnology and the First Affiliated Hospital Department of Radiation Oncology, Zhejiang University School of Medicine, Hangzhou, China; Joint Institute for Genetics and Genome Medicine between Zhejiang University and University of Toronto, Zhejiang University, Hangzhou, China. Electronic address:

Background & Aims: The early prognosis of hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is important to decrease its high mortality. This study aims to develop a new simplified prognostic score to accurately predict the outcome of these patients.

Methods: The prospective clinical data of 2409 hospitalized patients with acute deterioration of HBV-related chronic liver disease were used to develop a new prognostic score that was validated by an external group.

Results: A total of 954 enrolled patients with HBV-ACLF were diagnosed based on the Chinese Group on the Study of Severe Hepatitis B-ACLF (COSSH-ACLF) criteria. Six predictive factors were significantly related to the 28-day mortality and constituted a new prognostic score (=1.649×ln(international normalized ratio)+0.457×hepatic encephalopathy score+0.425×ln(neutrophil)+0.396×ln(total bilirubin)+0.576×ln(serum urea)+0.033×age). The C-indices of the new score for 28-/90-day mortality (0.826/0.809) were significantly higher than those of four other scores (COSSH-ACLFs, 0.793/0.784; CLIF-C ACLFs, 0.792/0.770; MELDs, 0.731/0.727; MELD-Nas, 0.730/0.726; all p<0.05). The prediction error rates of the new score for 28-day mortality were significantly lower than those of the COSSH-ACLFs (15.9%), CLIF-C ACLFs (16.3%), MELDs (35.3%) and MELD-Nas (35.6%). The probability density function evaluation and risk stratification of the new score also showed the highest predictive values for mortality. The external group further validated these results.

Conclusion: The new prognostic score based on six predictors without an assessment of organ failure can accurately predict and easily stratify the short-term mortality of patients with HBV-ACLF and might be used for early prognosis to decrease the high mortality.

Lay Summary: Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a complex syndrome with a high short-term mortality rate. We developed a simplified prognostic score for these patients based on a prospective multicentre cohort that showed the best predictive performance compared with four other generic prognostic scores (COSSH-ACLFs, CLIF-C ACLFs, MELDs and MELD-Nas).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhep.2021.05.026DOI Listing
June 2021

The prevalence of Toxoplasma gondii in sheep in China: A systematic review and meta-analysis.

Res Vet Sci 2021 May 25;138:19-29. Epub 2021 May 25.

College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China. Electronic address:

Toxoplasmosis is a zoonotic disease caused by Toxoplasma gondii, which is widespread in warm-blooded animals and humans. Currently, many studies regarding T. gondii infection in sheep have been published worldwide. However, there is no meta-analysis of sheep infection in China. In this study, five databases were used to retrieve articles related to T. gondii in China. A total of 59 studies from 1987 to June 30, 2020, have been included. We estimated that the combined prevalence of T. gondii in the selected period was 8.5% (3197/28,099). In the analysis of publication year, the lowest positive rate after 2010 was 7.4% (1883/17,313). Geographically, the highest prevalence of T. gondii in sheep was recorded in Southwest China 19.2% (490/2080). Analysis according to age showed that the prevalence of infection in sheep older than 12 months was significantly (P < 0.05) higher than that in younger animals. The prevalence of T. gondii in sheep farmed by farmers was 7.2% (566/6336), which was higher than 5.3% (660/3121) of large-scale farming, suggesting a role for the feeding and husbandry. We also analyzed the impact of different geographic and climatic factors on the prevalence of T. gondii infection in sheep. The results showed that the prevalence was higher in low altitude, warm, humid, and high rainfall areas. We suggest that appropriate control programs should be formulated according to the differences in reproduction patterns and geographical conditions in different regions to reduce the prevalence of T. gondii among sheep in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.rvsc.2021.05.016DOI Listing
May 2021

Soil capacity of intercepting different rainfalls across subtropical plantation: Distinct effects of plant and soil properties.

Sci Total Environ 2021 Aug 16;784:147120. Epub 2021 Apr 16.

University of Calgary, Calgary T2N1N4, Canada.

Forest management practices play an important role in soil water conservation. However, the soil water-holding capacity and associated drivers under different management practices remain uncertain, especially when the precipitation varies substantially at the regional scale. Here, we used hydrogen stable isotope to explore the contribution of rainfall to soil water (CRSW) under light, moderate and heavy precipitation in Pinus massoniana plantations with multiple management practices (pure stand, mixed stand, understory removal, light-intensity thinning and high-intensity thinning) in subtropical China. We further used variation partitioning analysis and structural equation modeling to identify the dominant driver affecting CRSW. Our results showed that after light rainfall, the highest CRSW (28.7%) was found in the high-intensity thinning plantation. However, after heavy rainfall, the high-intensity thinning plantation received the lowest CRSW (43.3%), while the mixed stand showed the highest CRSW (67.1%). These results demonstrated that the mixed stand of P. massoniana had a stronger capacity for soil water conservation, whereas high-intensity thinning showed poorer capacity. Furthermore, our results revealed that plant properties (i.e., tree, root and litter biomass) were the dominant controls of the CRSW under light rainfall, while soil properties (i.e., bulk density, total porosity, field capacity) were the primary drivers under moderate and heavy rainfall, indicating that the determinants influencing plantation capacity for intercepting rainfall vary with different levels of precipitation. These results highlight the importance of the level of precipitation in determining the dominant driver of CRSW. More importantly, these results suggest that the mixed stand, rather than high-intensity thinning, was better forest management since the former has a higher capacity for intercepting heavy rainfall.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2021.147120DOI Listing
August 2021

[Effect of electroacupuncture on learning-memory ability and expression of IL-1β, IL-6 and TNF-α in hippocampus and spleen in mice with Alzheimer's disease].

Zhen Ci Yan Jiu 2021 May;46(5):353-61

School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

Objective: To explore the effect of electroacupuncture (EA) on the ability of spatial learning-memory and the expression of IL-1β, IL-6 and TNF-α in the hippocampus and spleen tissues and the number of hippocampal neurons and spleen lymphocytes in Alzheimer's disease (AD) mice, so as to study its mechanisms underlying improvement of AD.

Methods: Twenty-four SAMP8 mice were randomly and equally divided into AD model, EA and medication groups, and 8 SAMR1 mice were used as the control group. EA (2 Hz, 0.1 mA) was applied to "Baihui"(GV20) and "Yintang"(EX-HN3) for 20 min in the EA group, and intragastric administration of donepezil hydrochloride (0.92 mg/kg) was applied in the medication group, once daily for 15 d. The learning-memory ability was determined by Morris water maze task, and the histopathological changes of hippocampus were observed after H.E. staining, followed by determining neurons and the number of splenic lymphocytes. The expression levels of IL-1β, IL-6 and TNF-α in the hippocampus and spleen were detected by immunohistochemistry and Western blot, respectively.

Results: After mode-ling, the escape latency of place navigation test in the Morris water maze, the spleen index, immunoactivity and expression levels of IL-1β, IL-6 and TNF-α proteins in the hippocampus and spleen tissues were significantly increased (<0.05, <0.01). Compared with the model group, the escape latency, spleen index, immunoactivity and expression levels of IL-1β, IL-6 and TNF-α proteins in both hippocampus and spleen were significantly down-regulated in the medication (except the escape latency) and EA groups (<0.01, <0.05). The effect of EA was evidently superior to that of medication in shortening the escape latency, lowering the spleen index, and immunoactivity of hippocampal IL-6 and splenic TNF-α immunoactivity (<0.01, <0.05). Outcomes of H.E. staining showed disordered arrangement of neurons with nuclear pyknosis or apoptosis in partial neurons in the hippocampus, and thickened and swollen spleen capsule tissue with loose structure and an increased number of lymphocytes in the model group, which was relatively milder in the EA and medication groups.

Conclusion: EA can improve the learning-memory ability of AD mice, which may be associated with its effect in relieving the inflammation reaction in the hippocampus and spleen tissues.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13702/j.1000-0607.200980DOI Listing
May 2021

GPC5 suppresses lung cancer progression and metastasis via intracellular CTDSP1/AhR/ARNT signaling axis and extracellular exosome secretion.

Oncogene 2021 Jun 2. Epub 2021 Jun 2.

Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.

Lung cancer is the leading cause of cancer-related death worldwide. Glypican-5 (GPC5) is a member of heparan sulfate proteoglycans, and its biological importance in initiation and progression of lung cancer remains controversial. In the present study, we revealed that GPC5 transcriptionally enhanced the expression of CTDSP1 (miR-26b host gene) via AhR-ARNT pathway, and such up-regulation of CTDSP1 intracellularly contributed to the inhibited proliferation of lung cancer cells. Moreover, exosomes derived from GPC5-overexpressing human lung cancer cells (GPC5-OE-derived exosomes) had an extracellular repressive effect on human lymphatic endothelial cells (hLECs), leading to decreased tube formation and migration. Comparison between GPC5-WT- and GPC5-OE-derived exosomes showed that miR-26b (embedded within introns of CTDSP1 gene) was significantly up-regulated in GPC5-OE-derived exosomes and critical to the influence on hLECs. On the mechanism, we demonstrated that miR-26b transferred into hLECs directly targeted to PTK2 3'-UTR and led to PTK2 down-regulation, resulting in defects in tube formation and migration of hLECs. By uncovering the regulation network among GPC5, miR-26b, miR-26b host gene (CTDSP1), and target gene (PTK2), our findings demonstrated that GPC5 functioned as a tumor suppressor in human lung cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41388-021-01837-yDOI Listing
June 2021

Microsatellite instability and Epstein-Barr virus combined with PD-L1 could serve as a potential strategy for predicting the prognosis and efficacy of postoperative chemotherapy in gastric cancer.

PeerJ 2021 18;9:e11481. Epub 2021 May 18.

Division of Clinical Research, First Hospital of Jilin University, Changchun, Jilin Province, China.

Background: Microsatellite instability (MSI) and Epstein-Barr virus (EBV)-positive molecular subtypes exhibit complex immune responses in gastric cancer (GC), and PD-L1 has emerged as a prognostic biomarker associated with the cancer immune microenvironment. This study aimed to determine the prognostic value of molecular subtypes and whether the addition of PD-L1 would accurately predict the prognosis and guide postoperative chemotherapy for GC patients.

Methods: We performed molecular subtyping of tissue microarray slides from 226 GC patients who were treated with radical gastrectomy. The MSI status and PD-L1 expression were evaluated through immunohistochemistry (IHC) and EBV status through situ hybridization. Multiplex polymerase chain reaction (PCR) was also performed on 50 cases to validate the accuracy of IHC in defining MSI status. Differences in overall survival (OS) were assessed using the Kaplan-Meier method, log-rank test and Cox proportional hazards regression model.

Results: Among the 226 GC patients, 52 (23.2%) patients were classified as the MSI subtype, 11 (4.9%) were EBV subtype, and 161 (71.9%) were MSS (Microsatellite stable) /EBV subtype according to TCGA analysis. Two patients were both positive for MSI and EBV infection. EBV cases showed higher PD-L1 positivity than MSI cases and MSS/EBV cases (81.8% 50.0% 35.4%, = 0.003). Compared with the non-MSS/EBV (MSI or EBV cases) subgroup, GC patients with MSS/EBV were associated with the worst outcomes (HR = 1.610, 95% CI [1.0462.479], = 0.031). MSS/EBV GCs alone could benefit from postoperative chemotherapy (HR = 0.452, 95% CI [0.2990.682], <0.001), and PD-L1-positive expression could also predict a better prognosis (HR = 0.612, 95% CI [0.3890.962], = 0.033) in this subgroup. Considering both chemotherapy efficacy and PD-L1 expression in the MSS/EBV subgroup, chemotherapy could improve the prognosis for PD-L1-negative MSS/EBV GCs (HR = 0.357, 95% CI [0.2170.587], <0.001) but not PD-L1-positive MSS/EBV GCs.

Conclusions: Molecular subtyping combined with PD-L1 expression could serve as a potential strategy to better predict prognosis and guide postoperative chemotherapy of GC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.11481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139270PMC
May 2021

Fine particulate matter-induced lung inflammation is mediated by pyroptosis in mice.

Ecotoxicol Environ Saf 2021 Aug 21;219:112351. Epub 2021 May 21.

International Collaborative Laboratory for Air Pollution Health Effects and Intervention, School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China. Electronic address:

Background: Exposure to ambient air-borne fine particulate matter (PM) increases respiratory morbidity and mortality. The mechanisms underlying PM-induced adverse effects remain unclear. This study aimed to uncover the molecular mechanisms of PM-induced lung toxicity using a mouse model.

Methods: Scanning electron microscopy and inductively coupled plasma mass spectrometry were used to examine and analyze PM morphology and element compositions, respectively. Twenty four male mice were randomly divided into three groups: control (PBS), PM (4.0 mg/kg b.w.), and PM + Z-YVAD-FMK. In the latter group, the pan-caspase inhibitor (Z-YVAD-FMK) was intraperitoneally injected into mice at a dose of 12.5 mg/kg body weight prior to intratracheal instillation of PM (4.0 mg/kg b.w.) every other day for a total of 3 times (n = 8 in each group). Bronchoalveolar lavage fluids (BALFs) were collected 24 h after the last instillation of PM. Levels of total proteins (TP), lactate dehydrogenase (LDH), IL-1β and IL-18 were analyzed for biomarkers of cell injury and inflammation. Additionally, histological alterations of lung tissues were assessed by hematoxylin-eosin staining. mRNA and protein expression of Caspase1, NLRP3 and GSDMD were examined by real-time fluorescent quantitative PCR and immunohistochemical staining.

Results: Exposure to PM increased levels of TP, LDH, IL-1β, IL-18 and inflammatory cell counts in lung. The mRNA and protein expression of Caspase1, NLRP3 and GSDMD were increased. Inhibition of the NALRP3/Caspase-1 signaling pathway ameliorated PM-induced lung injury and inflammation, partially through suppressing pyroptosis in lung.

Conclusion: PM exposure induces lung injury and inflammation, which is mediated by the NALRP3/Caspase-1 signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112351DOI Listing
August 2021

Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a.

Cell Discov 2020 May 19;6(1):30. Epub 2020 May 19.

Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, 200031, China.

CRISPRs are a promising tool being explored in combating exogenous retroviral pathogens and in disabling endogenous retroviruses for organ transplantation. The Cas12a and Cas13a systems offer novel mechanisms of CRISPR actions that have not been evaluated for retrovirus interference. Particularly, a latest study revealed that the activated Cas13a provided bacterial hosts with a "passive protection" mechanism to defend against DNA phage infection by inducing cell growth arrest in infected cells, which is especially significant as it endows Cas13a, a RNA-targeting CRISPR effector, with mount defense against both RNA and DNA invaders. Here, by refitting long terminal repeat retrotransposon Tf1 as a model system, which shares common features with retrovirus regarding their replication mechanism and life cycle, we repurposed CRISPR-Cas12a and -Cas13a to interfere with Tf1 retrotransposition, and evaluated their different mechanisms of action. Cas12a exhibited strong inhibition on retrotransposition, allowing marginal Tf1 transposition that was likely the result of a lasting pool of Tf1 RNA/cDNA intermediates protected within virus-like particles. The residual activities, however, were completely eliminated with new constructs for persistent crRNA targeting. On the other hand, targeting Cas13a to Tf1 RNA intermediates significantly inhibited Tf1 retrotransposition. However, unlike in bacterial hosts, the sustained activation of Cas13a by Tf1 transcripts did not cause cell growth arrest in S. pombe, indicating that virus-activated Cas13a likely acted differently in eukaryotic cells. The study gained insight into the actions of novel CRISPR mechanisms in combating retroviral pathogens, and established system parameters for developing new strategies in treatment of retrovirus-related diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41421-020-0164-0DOI Listing
May 2020

Anti-PD-1 Immunotherapy and Bee Venom for Relapsed and Refractory Liposarcoma: A Case Report.

Front Oncol 2021 29;11:668992. Epub 2021 Apr 29.

Department of Oncology, Hangzhou Red Cross Hospital, Hangzhou, China.

Cancer immunotherapies, including immune checkpoint inhibitors, elicit long-term clinical responses but many cancer patients do not respond. Intensive efforts are therefore underway to identify additional immune pathways that may be modulated to enhance the efficacy of existing immunotherapies. Bee venom strongly stimulates the immune system, and is used as a complementary therapy to treat cancer pain in patients with advanced tumors in China. Bee venom contains several allergenic protease inhibitors and peptides. It triggers hypersensitivity reactions; that is, it is an immune system agonist. The generation of a spontaneous T cell response against tumor-associated antigens requires innate immune activation; this drives type I interferon production. We report a patient with a relapsed and refractory liposarcoma who had undergone several operations, chemotherapies, and radiotherapies. The tumor was large. The patient had attained the maximum radiation exposure dose. The tumor was resistant to chemotherapy and was infiltrating the pericardium, lungs, and diaphragm. The patient was a poor candidate for resection. He thus received apitherapy (a combination of bee venom and acupuncture) to control pain; then apatinib (an anti-angiogenic drug) was given to inhibit tumor growth but was terminated early because the patient could not tolerate the side effects. Subsequently, a programmed death 1 inhibitor was combined with apitherapy. Bee venom served as an innate immune system agonist promoting immune cell priming and recruitment in the tumor microenvironment. The patient was finally able to undergo radical liposarcoma resection, and no evidence of recurrence was found at re-examination 16 months after surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.668992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8117145PMC
April 2021

Enhanced removal of cadmium from wastewater with coupled biochar and Bacillus subtilis.

Water Sci Technol 2021 May;83(9):2075-2086

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China E-mail:

Shortcomings of individual biochar or microbial technologies often exist in heavy metal removal from wastewater and may be circumvented by coupled use of biochar and microorganisms. In this study, Bacillus subtilis and each of three biochars of different origins (corn stalk, peanut shell, and pine wood) were coupled forming composite systems to treat a cadmium (Cd, 50 mg/L) wastewater formulated with CdCl in batch tests. Biochar in composite system enhanced the activity and Cd adsorption of B. subtilis. Compared with single systems with Cd removal up to 33%, the composite system with corn stalk biochar showed up to 62% Cd removal, which was greater than the sum of respective single B. subtilis and biochar systems. Further analysis showed that the removal of Cd by the corn stalk composite system could be considered to consist of three successive stages, that is, the biochar-dominant adsorption stage, the B. subtilis-dominant adsorption stage, and the final biofilm formation stage. The final stage may have provided the composite system with the ability to achieve prolonged steady removal of Cd. The biochar-microorganism composite system shows a promising application for heavy metal wastewater treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2166/wst.2021.138DOI Listing
May 2021

Paternal USP26 mutations raise Klinefelter syndrome risk in the offspring of mice and humans.

EMBO J 2021 May 12:e106864. Epub 2021 May 12.

Center for Reproductive Medicine, Cheeloo College of Medicine, Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, China.

Current understanding holds that Klinefelter syndrome (KS) is not inherited, but arises randomly during meiosis. Whether there is any genetic basis for the origin of KS is unknown. Here, guided by our identification of some USP26 variations apparently associated with KS, we found that knockout of Usp26 in male mice resulted in the production of 41, XXY offspring. USP26 protein is localized at the XY body, and the disruption of Usp26 causes incomplete sex chromosome pairing by destabilizing TEX11. The unpaired sex chromosomes then result in XY aneuploid spermatozoa. Consistent with our mouse results, a clinical study shows that some USP26 variations increase the proportion of XY aneuploid spermatozoa in fertile men, and we identified two families with KS offspring wherein the father of the KS patient harbored a USP26-mutated haplotype, further supporting that paternal USP26 mutation can cause KS offspring production. Thus, some KS should originate from XY spermatozoa, and paternal USP26 mutations increase the risk of producing KS offspring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.15252/embj.2020106864DOI Listing
May 2021

Dilatation and curettage versus lesion resection in the treatment of cesarean-scar-pregnancy: A systematic review and meta-analysis.

Taiwan J Obstet Gynecol 2021 May;60(3):412-421

Department of Obstetics & Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

This meta-analysis was performed to compare the efficacy and safety of dilatation and curettage (D&C) (simply D&C or combined with other treatments) and lesion resection for cesarean scar pregnancy (CSP). A search of English and Chinese databases from 2010 to 2019 was conducted. Thirty one studies were retrieved including sixteen random controlled and fifteen case controlled trials. Compared with abdominal resection surgery(ARS) and vaginal resection surgery(VRS), uterine artery embolization(UAE)+D&C has no obvious difference in curative effect and safety (UAE + D&C versus ARS: Cure rate(CR): P = 0.076, time for menstruation recovery/β-HCG normalization: P = 0.545/0.949,Blood loss: P = 0.005, adverse event: P = 0.420; versus VRS: CR: P = 0.085, time for menstruation recovery/β-HCG normalization: P < 0.001/P = 0.031,Blood loss: P = 0.902, adverse event: P = 0.249). UAE + D&C associated with lower blood loss and less postoperative complication than laparoscopic resection surgery(LRS), but LRS take more advantages in terms of the curative effect (CR: P = 0.047, time for menstruation recovery/β-HCG normalization: P = 0.352/0.103). The efficacy and safety of VRS are better than D&C, methotrexate (MTX) + D&C (D&C versus VRS: CR: P < 0.001, time for β-HCG normalization: P = 0.363,blood loss: P < 0.001, adverse event: P = 0.046; MTX + D&C versus VRS: CR: P < 0.001, time for menstruation recovery/β-HCG normalization: P < 0.001/P = 0.005, blood loss: P < 0.001, adverse event: P < 0.001). Lesion resection had advantages in shorter time for menstrual recovery/β-HCG normalization and less adverse events, lower failure rate over the administration of D&C treatments. In detail, the curative effect of UAE + D&C is similar to ARS and VRS, but inferior to LRS, while the safety of UAE + D&C is better than LRS. The efficacy and safety of simply D&C and MTX + D&C are not as good as VRS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tjog.2021.03.006DOI Listing
May 2021

Associations of short-term PM exposures with nasal oxidative stress, inflammation and lung function impairment and modification by GSTT1-null genotype: A panel study of the retired adults.

Environ Pollut 2021 Apr 24;285:117215. Epub 2021 Apr 24.

School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province, 453003, China. Electronic address:

PM (particulate matter ≤2.5 μm in aerodynamic diameter) is a major urban air pollutant worldwide. Its effects on the respiratory system of the susceptible population have been less characterized. This study aimed to estimate the association of short-term PM exposure with respiratory outcomes of the retired adults, and to examine whether these associations were stronger among the subjects with GSTT-null genotype. 32 healthy subjects (55-77 years) were recruited for five follow-up examinations. Ambient concentrations of PM were monitored consecutively for 7 days prior to physical examination. Pulmonary outcomes including forced vital capacity (FVC), forced expiratory volume in 1 s (FEV), peak expiratory flow (PEF), and fractional exhaled nitric oxide (FeNO), and nasal fluid concentrations of 8-epi-prostaglandin F2 alpha (8-epi-PGF2α), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8) and IL-1β were measured. A linear mixed-effect model was introduced to evaluate the associations of PM concentrations with respiratory outcomes. Additionally, GSTT1 genotype-based stratification was performed to characterize modification on PM-related respiratory outcomes. We found that a 10 μg/m increase in PM was associated with decreases of 0.52 L (95% confidence interval [CI]: -1.04, -0.002), 0.64 L (95% CI: -1.13, -0.16), 0.1 (95% CI: -0.23, 0.04) and 2.87 L/s (95% CI: -5.09, -0.64) in FVC, FEV, FEV/FVC ratio and PEF at lag 2, respectively. Meanwhile, marked increases of 80.82% (95% CI: 5.13%, 156.50%) in IL-8, 77.14% (95% CI: 1.88%, 152.40%) in IL-1β and 67.87% (95% CI: 14.85%, 120.88%) in 8-epi-PGF2α were observed as PM concentration increased by 10 μg/m at lag 2. Notably, PM-associated decreases in FVC and PEF and increase in FeNO were stronger among the subjects with GSTT1-null genotype. In summary, short-term exposure to PM is associated with nasal inflammation, oxidative stress and lung function reduction in the retired subjects. Lung function reduction and inflammation are stronger among the subjects with GSTT1-null genotype.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.117215DOI Listing
April 2021

Mussel-inspired surface functionalization of polyamide microfiltration membrane with zwitterionic silver nanoparticles for efficient anti-biofouling water disinfection.

J Colloid Interface Sci 2021 Sep 20;598:302-313. Epub 2021 Apr 20.

National Engineering Research Center of Clean Technology in Leather Industry, College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China; The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China. Electronic address:

Mature microfiltration (MF) membrane is a low-cost, effective, and promising technology to provide affordable purified water for people living in developing countries. However, the lack of disinfection ability and inherent membrane fouling problems have seriously restricted the large-scale application of conventional MF treatment system in producing safe drinking water. In this work, zwitterionic silver nanoparticles (AgNPs) with surface modification of poly(carboxybetaine acrylate-co-dopamine methacryamide) (PCBDA) copolymers were robustly immobilized onto commercial polyamide MF membrane via mussel-inspired chemistry for water disinfection. The designed microfiltration membrane, named as [email protected], exhibited integrated properties of high and stable payload of AgNPs, broad-spectrum anti-adhesive and antimicrobial activities, and easy removal of inactivated microbial cells from membrane surface. Ascribing to the synergetic effect of anti-adhesive and antimicrobial features brought by zwitterionic [email protected], the biofilms growth on polyamide membrane surface was significantly inhibited, which showed potential access to achieve long-term biofouling resistance and maintain water flux for conventional MF membrane. As water disinfection device, these attributes enabled [email protected] to effectively disinfect the model and natural bacteria-contaminated water.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.04.040DOI Listing
September 2021

LINC00958 promotes endometrial cancer cell proliferation and metastasis by regulating the miR-145-3p/TCF4 axis.

J Gene Med 2021 Apr 22:e3345. Epub 2021 Apr 22.

Department of Gynaecology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, Liaoning, China.

Background: Long noncoding RNAs (lncRNAs) exert an essential regulatory role in cancer progression. This work focuses on the role of LINC00958 in endometrial cancer (EC).

Methods: LINC00958 expression in EC tissues was examined by GEPIA database and TCGA-UCEC dataset. LINC00958, miR-145-3p, and TCF4 mRNA expression levels in EC tissues and cells were examined by qRT-PCR. Western blot was employed to determine TCF4, E-cadherin, and N-cadherin protein expression levels. After LINC00958 was overexpressed or silenced, cell proliferation was determined using Cell Counting Kit 8 (CCK-8) and bromodeoxyuridine (BrdU) incorporation experiments. Cell migration and invasion were examined by Transwell experiment. Dual-luciferase reporter gene or RNA immunoprecipitation (RIP) experiments were executed to validate the targeting relationships among LINC00958 and miR-145-3p and TCF4. The effects of LINC00958 on EC cell proliferation and metastasis were investigated in vivo using a nude mouse subcutaneous graft model and a caudal vein injection model.

Results: LINC00958 was remarkably upmodulated in EC. Moreover, its overexpression was strongly linked to unfavorable overall survival of the patients. Functional experiments confirmed that in vitro knockdown of LINC00958 suppressed EC cell proliferation and metastasis. LINC00958 was validated to decoy miR-145-3p and repressed its expression, and TCF4 was uncovered to be a target gene of miR-145-3p and negatively modulated by miR-145-3p. Furthermore, the function of LINC00958 was dependent on its regulation of miR-145-3p and TCF4.

Conclusions: LINC00958 acts as an oncogenic lncRNA to regulate EC progression by modulating the miR-145-3p/TCF4 axis. Knockdown of LINC00958 impedes tumor growth and metastasis in vitro and in vivo, opening a new avenue for therapeutic intervention.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jgm.3345DOI Listing
April 2021

Ferulic Acid Ameliorates Atherosclerotic Injury by Modulating Gut Microbiota and Lipid Metabolism.

Front Pharmacol 2021 25;12:621339. Epub 2021 Mar 25.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.

Atherosclerosis is a leading cause of death worldwide. Recent studies have emphasized the significance of gut microbiota and lipid metabolism in the development of atherosclerosis. Herein, the effects and molecular mechanisms involving ferulic acid (FA) was examined in atherosclerosis using the ApoE-knockout (ApoE, c57BL/6 background) mouse model. Eighteen male ApoE mice were fed a high-fat diet (HFD) for 12 weeks and then randomly divided into three groups: the model group, the FA (40 mg/kg/day) group and simvastatin (5 mg/kg/day) group. As results, FA could significantly alleviate atherosclerosis and regulate lipid levels in mice. Liver injury and hepatocyte steatosis induced by HFD were also mitigated by FA. FA improved lipid metabolism involving up-regulation of AMPKα phosphorylation and down-regulation of SREBP1 and ACC1 expression. Furthermore, FA induced marked structural changes in the gut microbiota and fecal metabolites and specifically reduced the relative abundance of , and which were positively correlated with serum lipid levels in atherosclerosis mice. In conclusion, we demonstrate that FA could significantly ameliorate atherosclerotic injury, which may be partly by modulating gut microbiota and lipid metabolism via the AMPKα/SREBP1/ACC1 pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fphar.2021.621339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026864PMC
March 2021

Blocking effect of fullerene nanoparticles (nC) on the plant cell structure and its phytotoxicity.

Chemosphere 2021 Sep 2;278:130474. Epub 2021 Apr 2.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China. Electronic address:

Blockage of nanoparticles on plant pore structures might produce phytotoxicity and affect plant uptake indirectly. This study examined the blocking and phytotoxic effects of fullerene nanoparticles (nC) on plants at the cellular level. The malondialdehyde content in plant was normal during nC exposure, implying that nC caused no acute phytotoxicity, while the normalized relative transpiration significantly decreased, showing that the pore structure of roots was seriously blocked by nC. High power optical microscopy and transmission electron microscope showed that root endothelial cells were squeezed, and inner wall structures were damaged by the extrusion of nanoparticles. Low nC concentrations inhibited root uptake of lindane, whereas high nC concentrations promoted root uptake of lindane, indicating that serious pore blocking by nC damaged root cell structure and hence ready transport of lindane from roots to shoots. Significant alterations of fatty acid (FA) saturation degree of root cell membrane indicated that nC led to phytotoxicity in the root cell membrane after long-term exposure and nC produced phytotoxicity in the process of blocking root pore structures and interfering with cell membrane fluidity. Moreover, the plant cell structures under phytotoxicity were more likely to be damaged mechanically by the extrusion of nanoparticles. These findings may be helpful to better understand the transport pathways of nanoparticles in plants, the phytotoxicity of nanoparticles and the potential risks of nanomaterials used in agriculture.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2021.130474DOI Listing
September 2021

Relationships of Rheumatoid Factor with Thickness of Retina and Choroid in Subjects without Ocular Symptoms Using Swept-Source Optical Coherence Tomography.

J Immunol Res 2021 24;2021:5547533. Epub 2021 Mar 24.

Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai, China.

Purpose: Researches have confirmed that the retinal and choroidal thickness in patients with autoimmune disease-associated uveitis displays significant changes. However, the relationships between rheumatoid factor (RF) and thickness of the retina and choroid in individuals without ocular manifestations remain unclear. The aim of this study is to assess the associations of RF with retinal and choroidal thickness.

Methods: The individuals enrolled in the cross-sectional research received full ocular examinations. The participants were classified as the RF (+) group (RF ≥ 15.0 IU/ml) and the RF (-) group (RF < 15.0 IU/ml) according to the serum RF titers. The thickness of the retina and choroid was measured by swept-source optical coherence tomography (SS-OCT).

Results: The study covered 65 right eyes of 65 individuals that are RF-positive and 130 right eyes of 130 age- and sex-matched individuals that are RF-negative. The RF (+) group showed decreased choroidal thickness that achieved statistical significance only in the outer inferior and outer temporal sectors, as compared to the RF (-) group. There was no statistically significant difference regarding the retinal thickness between the two groups. Pearson's correlation analysis revealed that the RF was significantly negatively related to the choroidal thickness in all areas. However, there was no significant correlation between the RF and the retinal thickness.

Conclusions: Serum RF titers are closely linked with choroidal thickness before the emergence of ocular symptoms. Research into the relationships may improve our understanding of the role of serum RF in the pathogenesis of uveitis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5547533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016561PMC
March 2021

Stress and psychological impact of the COVID-19 outbreak on the healthcare staff at the fever clinic of a tertiary general hospital in Beijing: a cross-sectional study.

BJPsych Open 2021 Apr 5;7(3):e76. Epub 2021 Apr 5.

Department of Emergency, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, China.

Background: It is important to maintain the psychological well-being of front-line healthcare staff during the coronavirus disease 2019 (COVID-19) pandemic.

Aims: To examine COVID-19-related stress and its immediate psychological impact on healthcare staff.

Method: All healthcare staff working in the fever clinic, from 20 January 2020 to 26 March 2020, of a tertiary general hospital were enrolled. Stress management procedures were in place to alleviate concerns about the respondents' own health and the health of their families, to help them adjust their work and to provide psychological support via a hotline. Qualitative interviews were undertaken and the Sources of Distress and the Impact of Event Scale-Revised (IES-R) were administered.

Results: Among the 102 participants (25 males; median age 30 years, interquartile range (IQR) = 27-36), the median IES-R total score was 3 (IQR = 0-8), and 6 participants (6.0%) scored above the cut-off on the IES-R (≥20). Safety and security were acceptable or better for 92 (90.2%) participants. The top four sources of distress were worry about the health of one's family/others at 0.88 (IQR = 0.25-1.25), worry about the virus spread at 0.50 (IQR = 0.00-1.00), worry about changes in work at 0.50 (IQR = 0.00-1.00) and worry about one's own health at 0.25 (IQR = 0.25-0.75). There was a moderate correlation between the IES-R score and the Sources of Distress score (rho = 0.501, P = 0.001).

Conclusions: The stress levels of healthcare staff in the fever clinic during the COVID-19 epidemic were not elevated. Physio-psychosocial interventions, including fulfilment of basic needs, activation of self-efficacy and psychological support, are helpful and worth recommending in fighting COVID-19.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1192/bjo.2021.32DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027548PMC
April 2021

Data Augmentation: Using Channel-Level Recombination to Improve Classification Performance for Motor Imagery EEG.

Front Hum Neurosci 2021 11;15:645952. Epub 2021 Mar 11.

Tianjin Artificial Intelligence Innovation Center (TAIIC), Tianjin, China.

The quality and quantity of training data are crucial to the performance of a deep-learning-based brain-computer interface (BCI) system. However, it is not practical to record EEG data over several long calibration sessions. A promising time- and cost-efficient solution is artificial data generation or data augmentation (DA). Here, we proposed a DA method for the motor imagery (MI) EEG signal called brain-area-recombination (BAR). For the BAR, each sample was first separated into two ones (named half-sample) by left/right brain channels, and the artificial samples were generated by recombining the half-samples. We then designed two schemas (intra- and adaptive-subject schema) corresponding to the single- and multi-subject scenarios. Extensive experiments using the classifier of EEGnet were conducted on two public datasets under various training set sizes. In both schemas, the BAR method can make the EEGnet have a better performance of classification ( < 0.01). To make a comparative investigation, we selected two common DA methods (noise-added and flipping), and the BAR method beat them ( < 0.05). Further, using the proposed BAR for augmentation, EEGnet achieved up to 8.3% improvement than a typical decoding algorithm CSP-SVM ( < 0.01), note that both the models were trained on the augmented dataset. This study shows that BAR usage can significantly improve the classification ability of deep learning to MI-EEG signals. To a certain extent, it may promote the development of deep learning technology in the field of BCI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnhum.2021.645952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990774PMC
March 2021

AKR1B10 confers resistance to radiotherapy via FFA/TLR4/NF-κB axis in nasopharyngeal carcinoma.

Int J Biol Sci 2021 3;17(3):756-767. Epub 2021 Feb 3.

Department of Laboratory Medicine, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Guangdong 518000, P.R China.

Nasopharyngeal carcinoma (NPC) is one kind of human head and neck cancers with high incidence in Southern China, Southeast Asia and North Africa. In spite of great innovations in radiation and chemotherapy treatments, the 5-year survival rate is not satisfactory. One of the main reasons is resistance to radiotherapy which leads to therapy failure and recurrence of NPC. The mechanism underlying remains to be fully elucidated. Aldo-keto reductase B10 (AKR1B10) plays a role in the formation and development of carcinomas. However, its role in resistance to radiotherapy of NPC is not clear. In this research, the relationships between AKR1B10 expression and the treatment effect of NPC patients, NPC cell survival, cell apoptosis, and DNA damage repair, as well as the effect and mechanism of AKR1B10 expression on NPC radioresistance were explored. A total of 58 paraffin tissues of NPC patients received radiotherapy were collected including 30 patients with radiosensitivity and 28 patients with radioresistance. The relationships between AKR1B10 expression and the treatment effect as well as clinical characteristics were analyzed by immuno-histochemical experiments, and the roles of AKR1B10 in cell survival, apoptosis and DNA damage repair were detected using the AKR1B10 overexpressed cell models. Furthermore the mechanism of AKR1B10 in NPC radioresistance was explored. Finally, the radioresistance effect of AKR1B10 expression was evaluated by the tumor xenograft model of nude mice and the method of radiotherapy. The results showed AKR1B10 expression level was correlated with radiotherapy resistance, and AKR1B10 overexpression promoted proliferation of NPC cells, reduced apoptosis and decreased cellular DNA damage after radiotherapy. The probable molecular mechanism is that AKR1B10 expression activated FFA/TLR4/NF-κB axis in NPC cells. This was validated by using the TLR4 inhibitor TAK242 to treat NPC cells with AKR1B10 expression, which reduced the phosphorylation of NF-κB. This study suggests that AKR1B10 can induce radiotherapy resistance and promote cell survival via FFA/TLR4/NF-κB axis in NPC, which may provide a novel target to fight against radiotherapy resistance of NPC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/ijbs.52927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975703PMC
February 2021

Author Correction: Application of Whole Genome Resequencing in Mapping of a Tomato Yellow Leaf Curl Virus Resistance Gene.

Sci Rep 2021 Mar 19;11(1):6917. Epub 2021 Mar 19.

Institute of Vegetable Crop, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-86636-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979778PMC
March 2021

Erythrocyte membrane-camouflaged nanoworms with on-demand antibiotic release for eradicating biofilms using near-infrared irradiation.

Bioact Mater 2021 Sep 1;6(9):2956-2968. Epub 2021 Mar 1.

State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, 400715, China.

The increase in the number of resistant bacteria caused by the abuse of antibiotics and the emergence of biofilms significantly reduce the effectiveness of antibiotics. Bacterial infections are detrimental to our life and health. To reduce the abuse of antibiotics and treat biofilm-related bacterial infections, a biomimetic nano-antibacterial system, RBCM-NW-G namely, that controls the release of antibiotics through near infrared was prepared. The hollow porous structure and the high surface activity of nanoworms are used to realize antibiotic loading, and then, biomimetics are applied with red blood cell membranes (RBCM). RBCM-NW-G, which retains the performance of RBCM, shows enhanced permeability and retention effects. Fluorescence imaging in mice showed the effective accumulation of RBCM-NW-G at the site of infection. In addition, the biomimetic nanoparticles showed a longer blood circulation time and good biocompatibility. Anti-biofilm test results showed damage to biofilms due to a photothermal effect and a highly efficient antibacterial performance under the synergy of the photothermal effect, silver iron, and antibiotics. Finally, by constructing a mouse infection model, the great potential of RBCM-NW-G in the treatment of in vivo infections was confirmed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bioactmat.2021.01.032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930507PMC
September 2021

The Role of Root Morphology and Pulling Direction in Pullout Resistance of Alfalfa Roots.

Front Plant Sci 2021 17;12:580825. Epub 2021 Feb 17.

College of Water Resources Science and Engineering, Taiyuan University of Technology, Taiyuan, China.

There is a growing consensus on soil conservation by mechanics of plant root system. In order to further study how root system exerts its mechanical properties during soil reinforcing process and which morphological indicator is suitable for reflecting pullout resistance, vertical pullout test (VPT) and 45° oblique pullout test (OPT) were performed on alfalfa ( L.) roots in the loess area. The results showed that the failure mode of alfalfa roots was pulling out in this study. The peak pullout resistance of the roots increased with root diameter, root length and root surface area, and power law relationships were observed between the pullout resistance and the morphological indices: root diameter, root length and root surface area. The maximum gray relational degree of the morphological indices was 0.841 (VPT) and 0.849 (OPT) for root surface area, suggesting that root surface area was a more significant root morphological index affecting root pullout resistance than root diameter and root length, and was more suitable for characterizing the difference in peak pullout resistance of roots with different size. The index could be used to validate the methods for predicting root pullout capacity. The value of peak pullout resistance was 17.2 ± 2.3 N in VPT test and 28.2 ± 3.8 N (mean ± SE) in OPT test, and a significant difference was observed between the two tests, which showed that the pulling direction significantly affected the peak pullout resistance of alfalfa roots. Vertical pullout test, giving the safety margin, was suggested to determine root pullout resistance for estimate of root reinforcement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.580825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934573PMC
February 2021

[Clinical efficacy of posterior percutaneous endoscopic cervical discectomy for single level cervical spondylopathy with intraspinal ossification].

Zhongguo Gu Shang 2021 Jan;34(1):20-5

Department of Pain, Huazhong University of Science and Technology Union Shenzhen Hospital, Shenzhen 518052, Guandong, China.

Objective: To evaluate the effectiveness and security of posterior percutaneous endoscopic cervical discectomy (PPECD) in the treatment of single level cervical spondylopathy with intraspinal ossification.

Methods: Twenty three patients with single level cervical spondylopathy with intraspinal ossification were treated by posterior percutaneous endoscopic cervical discectomy between August 2017 and July 2019. There were 16 males and 7 females, aged from 29 to 74 years old with an average of (50±13) years.The disease duration were 3 to 120 months with a median of 6 months. There were 9 cases of cervical spondylotic radiculopathy, 6 cases of cervical spondylotic myelopathy, and 8 cases of mixed cervical spondylopathy. According to the characteristics of ossification, 17 cases were osteophytes on the posterior edge of the vertebral body;3 cases were protrusion ossification;3 cases were posterior longitudinal ligament ossification. According to the position of ossification in spinal canal, 14 cases were medial and lateral type, 5 cases were central type, and 4 cases were mixed type. Posterior percutaneous cervical endoscopic cervical discectomy in patients performed by the same surgeon. Japanese Orthopaedic Association (JOA) score and visual analogue scale(VAS) were compared separately before and after operation. At 3 months after operation, clinical effect was assessed according to modified Macnab standard.

Results: All operations were successful. The operative time was 30 to 155 (69.1±27.2) min. The bedridden time was 2 to 3(3.0±0.9) h, length of postoperative hospitalization was 2 to 7(4.1± 1.5) d. Three dimensional CT reconstruction of the cervical spine at 3 days after operation showed that ossified tissue of 13 cases were completely removed, and 10 cases were left after operation, and the residual was located at the posterior edge and/or center of the upper vertebral body. VAS score at discharge from hospital was significantly lower than that before operation (=9.35, <0.001), and 21 cases had a score of 0 to 3. Postoperative JOA score was significantly higher (=7.29, <0.001). At 3 months after operation, according to modified Macnab standard to evaluate clinical effect, 18 cases got exellent results, 4 good and 1 fair, with an excellent and good rate of 95.6%(22/23).

Conclusion: For an experienced surgeon, percutaneous posterior cervical endoscopic discectomy is safe and reliable in treating single level cervical spondylopathy with intraspinal ossification, and can obtain good clinical results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12200/j.issn.1003-0034.2021.01.005DOI Listing
January 2021

Age-related changes in expression of lysine48 and lysine63 ubiquitin linkages in dopamine neurons of midbrain in mice.

Neuroreport 2021 May;32(7):569-576

School of Basic Medical Sciences.

Ubiquitination of target proteins is mediated via different ubiquitin lysine (K) linkages and determines the protein fates. In particular, K48 ubiquitin linkage targets proteins for degradation, whereas K63 ubiquitin linkage plays a nondegradative role. Parkinson's disease is an age-onset neurodegenerative disorder, which shows selective loss of dopamine neurons in substantia nigra pars compacta (SNC) and ubiquitinated protein aggregates. However, age-related expression of K48 and K63 ubiquitin linkages in SNC dopamine neurons remains elusive. We thus sought to explore the expression of K48 and K63 ubiquitin linkages in dopamine neurons in SNCs of mice at different ages with morphological and biochemical assays. Here our results indicated that in 5-week-old mice, dopamine neurons presented higher levels of K48 and K63 ubiquitin linkages than nondopamine neural cells. Aging promoted the formation of protein aggregates that are positive for both K48 and K63 ubiquitin linkages, together with tyrosine hydroxylase, a dopamine neuron marker. Moreover, 21-month-old mice showed fewer neural cells and tyrosine hydroxylase positive neurons in the SNCs than younger mice. Through biochemical analysis, the 21-month-old mice were shown to express more K48 ubiquitin linkages and less tyrosine hydroxylase and NeuN than the 5-week-old mice. These results suggest the first time that expression of K48 and K63 ubiquitin lysine linkages in midbrain dopamine neurons is age-related and may be involved in the loss of dopamine neurons.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/WNR.0000000000001626DOI Listing
May 2021

Characterization of exopolysaccharides produced by Weissella confusa XG-3 and their potential biotechnological applications.

Int J Biol Macromol 2021 May 27;178:306-315. Epub 2021 Feb 27.

Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Harbin, Heilongjiang 150500, PR China; Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, Heilongjiang 150080, PR China. Electronic address:

In this study, exopolysaccharides (EPSs) produced by Weissella confusa XG-3 were characterized. The monosaccharide composition of XG-3 EPS was determined to include glucose according to GC data, and its molecular weight was 3.19 × 10 Da, as determined by HPLC. Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS. The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran. X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran. XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C. The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration. The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose. XG-3 dextran stimulated the growth of Lactobacillus spp. and Bifidobacterium spp. These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.02.182DOI Listing
May 2021

Sugarcane/peanut intercropping system improves physicochemical properties by changing N and P cycling and organic matter turnover in root zone soil.

PeerJ 2021 16;9:e10880. Epub 2021 Feb 16.

Guangxi Academy of Agricultural Sciences, Cash Crops Research Institute, Nanning, Guangxi, China.

Background: The sugarcane/peanut intercropping system is a specific and efficient cropping pattern in South China. Intercropping systems change the bacterial diversity of soils and decrease disease rates. It can not only utilized light, heat, water and land resources efficiently, but also increased yield and economic benefits of farmers.

Methods: We determined soil nutrients, enzymes and microbes in sugarcane/peanut intercropping system, and analyzed relevance of the soil physicochemical properties and the genes involved in N and P cycling and organic matter turnover by metagenome sequencing.

Results: The results showed that sugarcane/peanut intercropping significantly boosted the content of total nitrogen, available phosphorus, total potassium, organic matter, pH value and bacteria and enhanced the activity of acid phosphatase compared to monocropping. Especially the content of available nitrogen, available phosphorus and organic matter increased significantly by 20.1%, 65.3% and 56.0% in root zone soil of IP2 treatment than monocropping treatment. The content of available potassium and microbial biomass carbon, as well as the activity of catalase, sucrase and protease, significantly decreased in intercropping root zone soil. Intercropping resulted in a significant increase by 7.8%, 16.2% and 23.0% in IS, IP1 and IP2, respectively, of the acid phosphatase content relative to MS. Metagenomic analysis showed that the pathways involved in carbohydrate and amino acid metabolism were dominant and more abundant in intercropping than in monocropping. Moreover, the relative abundances of genes related to N cycling (, , ), P cycling (, ) and organic matter turnover () were higher in the intercropping soil than in the monocropping soil. The relative abundance of and were 25.5% and 13.8% higher in the IP2 treatment respectively,and was higher in IS treatment compared to the monocropping treatment. Genes that were significantly related to phosphorus metabolism and nitrogen metabolism (, , ) were more abundant in intercropping than in monocropping.

Conclusion: The results of this study indicate that the intercropping system changed the numbers of microbes as well as enzymes activities, and subsequently regulate genes involved in N cycling, P cycling and organic matter turnover. Finally, it leads to the increase of nutrients in root zone soil and improved the soil environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.10880DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894120PMC
February 2021

Tissue inhibitor of metalloproteinase-1 (TIMP-1) as a prognostic biomarker in gastrointestinal cancer: a meta-analysis.

PeerJ 2021 16;9:e10859. Epub 2021 Feb 16.

Division of Clinical Research, First Hospital of Jilin University, Changchun, Jilin, China.

Background: Tissue inhibitor of metalloproteinase 1 (TIMP-1) has recently been shown to be dependent on or independent of Matrix metalloproteinases (MMPs) in its roles in tumorigenesis and progression. This appreciation has prompted various studies assessing the prognostic value of TIMP-1 in patients with gastrointestinal cancer, however, the conclusions were still inconsistent. The aim of this study was to assess the prognostic value of TIMP-1-immunohistochemistry (IHC) staining and pretreatment serum/plasma TIMP-1 level in gastrointestinal cancer survival as well as the association between TIMP-1 and clinicopathologic features.

Methods: The meta-analysis was registered in the International Prospective Register of Systematic Reviews (PROSPERO; Registration NO. CRD42020185407) and followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement. A highly sensitive literature search was performed in electronic databases including PubMed, EMBASE and the Cochrane Library. Heterogeneity analysis was conducted using both chi-square-based Q statistics and the I test. The pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were calculated to assess the prognostic value of TIMP-1 using the fixed-effects model. Odds ratios (ORs) with 95% CIs were calculated to evaluate the associations between TIMP-1 and clinicopathological characteristics. The meta-analysis was conducted using STATA 12.0 software.

Results: A total of 3,958 patients from twenty-two studies were included in the meta-analysis. Elevated TIMP-1 levels were significantly associated with poor survival in gastrointestinal cancer (TIMP-1-IHC staining: HR = 2.04, 95% CI [1.59-2.61],  = 35.7%, = 0.156; pretreatment serum/plasma TIMP-1 levels: HR = 2.02, 95% CI [1.80-2.28],  = 0%, = 0.630). Moreover, clinicopathological parameter data analysis showed that elevated TIMP-1 levels were significantly associated with lymph node metastasis (N1/N2/N3 vs N0: OR = 2.92, 95% CI [1.95-4.38]) and higher TNM stages (III/IV vs I/II: OR = 2.73, 95% CI [1.23-6.04]).

Conclusion: Both TIMP-1-positive IHC staining and high serum/plasma TIMP-1 levels are poor prognostic factors for the survival of gastrointestinal cancer. In addition, TIMP-1 overexpression was correlated with more advanced clinicopathological features.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.10859DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7894117PMC
February 2021

Nanozyme-based medicine for enzymatic therapy: progress and challenges.

Biomed Mater 2021 Feb 18. Epub 2021 Feb 18.

Institute of Biophysics Chinese Academy of Sciences, 15 Datun Road, Beijing, Beijing, 100101, CHINA.

Nanozymes are nanomaterials with enzyme-like characteristics. As a new generation of artificial enzymes, nanozymes have the advantages of low cost, good stability, simple preparation, and easy storage, allowing them to overcome many of the limitations of natural enzymes in enzymatic therapy. Currently, most reported nanozymes exhibit oxidoreductase-like activities and can regulate redox balance in cells. Nanozymes with superoxide dismutase and catalase activity can be used to scavenge reactive oxygen species (ROS) for cell protection, while those with peroxidase and oxidase activity can generate ROS to kill harmful cells, such as tumor cells and bacteria. In this review, we summarize recent progress in nanozyme-based medicine for enzymatic therapy and highlight the opportunities and challenges in this field for future study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1748-605X/abe7b4DOI Listing
February 2021