Publications by authors named "Jing Hou"

388 Publications

α‑rhamnrtin‑3‑α‑rhamnoside exerts anti‑inflammatory effects on lipopolysaccharide‑stimulated RAW264.7 cells by abrogating NF‑κB and activating the Nrf2 signaling pathway.

Mol Med Rep 2021 Nov 15;24(5). Epub 2021 Sep 15.

Department of Chinese Medicine, School of Pharmaceutical Science, Shanxi Medical University, Jinzhong, Shanxi 030600, P.R. China.

α‑rhamnrtin‑3‑α‑rhamnoside (ARR) is the principal compound extracted from Franch. & Sav. However, its underlying pharmacological properties remain undetermined. Inflammation is a defense mechanism of the body; however, the excessive activation of the inflammatory response can result in physical injury. The present study aimed to investigate the effects of ARR on lipopolysaccharide (LPS)‑induced RAW264.7 macrophages and to determine the underlying molecular mechanism. A Cell Counting Kit‑8 assay was performed to assess cytotoxicity. Nitric oxide (NO) production was measured via a NO colorimetric kit. Levels of prostaglandin E2 (PGE) and proinflammatory cytokines, IL‑1β and IL‑6, were detected using ELISAs. Reverse transcription‑quantitative (RT‑q)PCR analysis was performed to detect the mRNA expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase‑2 (COX‑2), IL‑6 and IL‑1β in LPS‑induced RAW246.7 cells. Western blotting, immunofluorescence and immunohistochemistry analyses were performed to measure the expression levels of NF‑κB and nuclear factor‑erythroid 2‑related factor 2 (Nrf2) signaling pathway‑related proteins to elucidate the molecular mechanisms of the inflammatory response. The results of the cytotoxicity assay revealed that doses of ARR ≤200 g/ml exhibited no significant effect on the viability of RAW264.7 cells. The results of the Griess assay demonstrated that ARR inhibited the production of NO. In addition, the results of the ELISAs and RT‑qPCR analysis discovered that ARR reduced the production of the proinflammatory cytokines, IL‑1β and IL‑6, as well as the proinflammatory mediators, PGE, iNOS and COX‑2, in LPS‑induced RAW264.7 cells. Immunohistochemical analysis demonstrated that ARR inhibited LPS‑induced activation of TNF‑associated factor 6 (TRAF6) and NF‑κB p65 signaling molecules, while reversing the downregulation of the NOD‑like receptor family CARD domain containing 3 (NLRC3) signaling molecule, which was consistent with the results of the western blotting analysis. Immunofluorescence results indicated that ARR reduced the increase of NF‑κB p65 nuclear expression induced by LPS. Furthermore, the results of the western blotting experiments also revealed that ARR upregulated heme oxygenase‑1, NAD(P)H quinone dehydrogenase 1 and Nrf2 pathway molecules. In conclusion, the results of the present study suggested that ARR may exert anti‑inflammatory effects by downregulating NF‑κB and activating Nrf2‑mediated inflammatory responses, suggesting that ARR may be an attractive anti‑inflammatory candidate drug.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2021.12439DOI Listing
November 2021

Carbonylative coupling of simple alkanes and alkenes enabled by organic photoredox catalysis.

Chem Commun (Camb) 2021 Sep 15. Epub 2021 Sep 15.

College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

A visible-light-driven direct carbonylative coupling of simple alkanes and alkenes the combination of a hydrogen atom transfer process and photoredox catalysis has been demonstrated. Employing the -alkoxyazinium salt as the oxidant and the precursor of an oxygen radical, a variety of α,β-unsaturated ketones could be obtained in a metal-free fashion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1cc04138jDOI Listing
September 2021

Soluble expression and purification of human β-defensin DEFB136 in Escherichia coli and identification of its bioactivity.

Protein Expr Purif 2021 Sep 2:105968. Epub 2021 Sep 2.

Department of Biology, College of Ecology, Lishui University, Lishui City, 323000, China.

Human β-defensins are an important family of innate host defense peptides with pleiotropic activities. Human β-defensin 36 (DEFB136) is a novel member of the β-defensin family which have not been characterized so far. In the present research, DEFB136 peptide was expressed successfully and purified using the IMPACT-TWIN 1 expression system. The purified DEFB136 peptide was identified by MALDI-TOF mass spectrometry and circular dichroism spectroscopy. While the recombinant DEFB136 peptide exhibited a broad spectrum of antimicrobial activity against E. coli, Staphylococcus aureus and Candida albicans strains, but had low cytotoxicity to human erythrocytes. In addition, the result of the octet assay showed that the DEFB136 had a high lipopolysaccharide (LPS)-binding affinity, suggesting the DEFB136 may be involved in immunoregulation through its LPS neutralization. These results may help lay the groundwork to understand better the complex interaction between innate host defense and the diversity of the defensin family.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.pep.2021.105968DOI Listing
September 2021

Exosomal transfer of miR-106a-5p contributes to cisplatin resistance and tumorigenesis in nasopharyngeal carcinoma.

J Cell Mol Med 2021 Sep 1. Epub 2021 Sep 1.

Guizhou university medical college, Guiyang, China.

Nasopharyngeal carcinoma (NPC), a subclass of cancers of the neck and head, is a predominant cause of cancer-associated death worldwide. Hence, there is a critical need for research into NPC-related treatment strategies. Cisplatin is a promising therapy option for NPCs and other cancers that is frequently utilized. Some patients acquire resistance to cisplatin therapy, which complicates the successful use of cisplatin treatment in NPCs. Although exosomal transfer of oncogenic miRNAs has been shown to improve recipient cell proliferation, metastasis and chemoresistance, the molecular mechanism behind this effect on NPC has yet to be fully understood. Exosomal microRNAs (miRNAs) from cisplatin-resistant cells were identified as significant mediators of chemoresistance in NPC cells in this investigation. Initially, we found that exosomal miR-106a-5p levels in the serum of chemoresistant and last-cycle patients were greater than in that of non-resistant and first-cycle patients. Also, exosomal miR-106a-5p enhanced the proliferative ability of NPC cells. Mechanistically, exosomal miR-106a-5p targets ARNT2, which further activates AKT phosphorylation, and thus promotes NPC cell proliferation, decreases apoptosis and in turn regulates tumorigenesis. We found similar results using in vivo NPC models, where exosomal miR-106a-5p through regulation of ARNT2 (aryl hydrocarbon receptor nuclear translocator 2) promoted tumorigenesis. Taken together, these findings indicate that exosomal miR-106a-5p could be a promising diagnostic biomarker and drug target for patients with NPC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jcmm.16801DOI Listing
September 2021

MRI-based radiomics nomogram for predicting temporal lobe injury after radiotherapy in nasopharyngeal carcinoma.

Eur Radiol 2021 Aug 31. Epub 2021 Aug 31.

Department of Diagnostic Radiology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 283 Tongzipo Road, Yuelu District, Changsha, 410013, Hunan, People's Republic of China.

Objectives: To develop and validate a magnetic resonance imaging (MRI)-based radiomics nomogram model combining radiomic features and clinical factors for the prediction of radiotherapy-induced temporal lobe injury (RTLI) in patients with nasopharyngeal carcinoma (NPC).

Methods: From 203 NPC cases receiving radiotherapy, 128 RTLI-positive and 278 RTLI-negative lobes were retrospectively analyzed. They were randomly divided into training (n = 285) and validation (n = 121) sets. Three hundred ninety-six texture features based on T2WI images were extracted from each temporal lobe. The minimum redundancy maximum relevance (mRMR) and the least absolute shrinkage and selection operator (LASSO) were used to reduce the dimension of the features and establish a radiomics signature model. Clinical risk factors and the radiomics signature were combined by multivariable logistic regression analysis to construct a radiomics nomogram model. We assessed the performance of the radiomics nomogram on discrimination, calibration, and clinical utility.

Results: The radiomics signature consisted of 14 selected features that were significantly associated with RTLI. In the training set, the radiomics nomogram model demonstrated a better predictive performance (AUC, 0.87; 95% CI, 0.82-0.91) than the radiomics model (AUC, 0.71; 95% CI, 0.65-0.78) and clinical model (AUC, 0.73; 95% CI, 0.67-0.79). These results were confirmed in the validation set. The radiomics nomogram model demonstrated good calibration and was clinically useful by decision curve analysis.

Conclusion: The radiomics nomogram model combining radiomics signatures and clinical factors is an effective method for the noninvasive prediction of RTLI in NPC patients after radiotherapy.

Key Points: • The radiomics model based on T2WI images at the end of intensity-modulated radiotherapy can predict radiotherapy-induced temporal lobe injury in patients with NPC. • Dosimetric factors can improve the prediction performance of the radiomics model in predicting radiotherapy-induced temporal lobe injury. • An MRI-based radiomics nomogram combining radiomics signatures and clinical factors had better prediction performance than both radiomics and clinical model for the prediction of radiotherapy-induced temporal lobe injury in patients with NPC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00330-021-08254-5DOI Listing
August 2021

Brusatol inhibits laryngeal cancer cell proliferation and metastasis via abrogating JAK2/STAT3 signaling mediated epithelial-mesenchymal transition.

Life Sci 2021 Aug 25;284:119907. Epub 2021 Aug 25.

School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi Province, China.

Aims: This study aimed at investigating the role of Brusatol (BR) on human laryngeal squamous carcinoma cell (Hep-2) to study its underlying mechanism through in vitro and in vivo approaches.

Materials And Method: In the present research, we employed various cell-based assays, such as cell proliferation, apoptosis, cell cycle assessment, migration and invasion assays were used to examine the anti-tumor effect of BR on Hep-2 cells. Immunohistochemistry (IHC), qRT-PCR and Western blotting were performed to study the underlying molecular mechanisms. To validate our in vitro findings we used a subcutaneous tumor-bearing model of Balb/c mice with Hep-2 cells of laryngeal carcinoma (LC) to study the inhibitory effect of BR on Hep-2 cells in vivo.

Key Findings: The results indicated that BR markedly inhibited the viability, migration and invasion capacity of Hep-2 cells, with no significant toxic effect on normal Human bronchial epithelial cell line (BEAS-2B). Also, BR induced cellular apoptosis by blocking the cells in S phase to suppress cell proliferation. Immunohistochemistry results revealed that BR inhibited the protein expression levels of epithelial-mesenchymal transition (EMT)-related markers. Mechanistically, western blotting results exhibited that BR could suppress the protein expression of both JAK2/STAT3 and their phosphorylation levels. Our in vivo experiments further validated the anti-tumor effect of BR on Hep-2 cells in vitro, where BR suppressed the growth of xenograft laryngeal tumor without apparent toxicity.

Significance: The present study highlights the anti-LC effect of BR by possibly abrogating JAK2/STAT3 signaling mediated EMT process. BR may be a promising therapeutic candidate for the treatment of LC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119907DOI Listing
August 2021

A method for evaluating indoor phthalate exposure levels in collected dust.

MethodsX 2021 17;8:101187. Epub 2020 Dec 17.

Tianjin Key Laboratory of Indoor Air Environmental Quality Control, School of Environmental Science and Engineering, Tianjin University, China.

This paper provides a method for evaluating indoor phthalate exposure levels in collected dust, including dust collection, chemical analysis and quality control. Indoor dusts were collected with a self-made equipment and pretreated by the Soxhlet extractor method. Six phthalates, i.e., diethyl phthalate (DEP), di-n-butyl phthalate (DnBP), di-isobutyl phthalate (DiBP), benzyl butyl phthalate (BBzP), di (2-ethylhexyl) phthalate (DEHP) and di-isononyl phthalate (DiNP) in the dust were analyzed by gas chromatography mass spectrometry (GC-MS). •Measures were taken to avoid background contamination of phthalates during sampling and chemical analysis to accurately evaluate the indoor phthalates exposure levels.•Filter sock and self-made mouthpiece mounted on vacuum cleaner were made of nylon and aluminum respectively.•Dust from surface of plastic products was avoided. The pretreatment of vessels and analysis of blank samples were conducted to reduce "noise" from background contamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mex.2020.101187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374174PMC
December 2020

Corrigendum: Foot-and-Mouth Disease Virus Capsid Protein VP1 Antagonizes TPL2-Mediated Activation of the IRF3/IFN-β Signaling Pathway to Facilitate the Virus Replication.

Front Immunol 2021 6;12:686494. Epub 2021 Aug 6.

State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, Lanzhou, China.

[This corrects the article .].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.686494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378326PMC
August 2021

Theoretical research on cage-like furazan-based energetic compounds and its derivatives.

J Mol Model 2021 Aug 10;27(9):243. Epub 2021 Aug 10.

School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.

In this manuscript, we reported the design and prediction of two furazan-based cage-like molecules and their derivatives using density function theory (DFT). The heat formation and detonation properties were calculated using Hess's law and Kamlet-Jacobs equations with the B3PW91 method. The molecular stability and geometry were analyzed using the M06-2X method, and molecular crystal structures were predicted based on Monte Carlo simulation, while chemical reactive sites were judged using the PBE0 method based on Fukui function. The theoretical calculation result proved that the designed molecules exhibit ideal symmetric cage-like geometry and show superior physicochemical and detonation properties. Compared with traditional energetic materials, the designed molecules display more positive solid heat formation and lower sensitivity. The designed molecules could be considered promising high energy density material candidates with potential synthesis and application value. Two designed molecules display superior detonation performance and ideal completely symmetric cage-like geometry, which were proved theoretically as a promising HEDM candidate. A series of derivatives also exhibited excellent crystal density and physicochemical properties, while with more stable structure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00894-021-04849-3DOI Listing
August 2021

sp. nov. and sp. nov., isolated from salt lake and saline soil.

Int J Syst Evol Microbiol 2021 Jul;71(7)

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.

Two halophilic archaeal strains, Gai3-2 and NJ-3-1, were isolated from salt lake and saline soil samples, respectively, collected in PR China. The 16S rRNA gene sequences of the two strains were 97.5% similar to each other. Strains Gai3-2 and NJ-3-1 had the highest sequence similarities to '' G22 (96.7 and 97.8%, respectively), and displayed similarities of 91.5-93.5% and 92.3-94.7%, respectively, to members. Phylogenetic analysis revealed that the two strains formed different branches and clustered tightly with '' G22 and members. The average nucleotide identity (ANI), in silico DNA-DNA hybridization (DDH) and amino acid identity (AAI) values between the two strains were 83.1, 26.9 and 77.9%, respectively, much lower than the threshold values proposed as a species boundary. These values between the two strains and '' G22 (ANI 77.9-78.2%, DDH 22.5-22.6% and AAI 68.8-69.3%) and members (ANI 77.53-77.63%, DDH 21.8-22.3% and AAI 68.4-69.4%) were almost identical, and much lower than the recommended threshold values for species delimitation. These results suggested that strains Gai3-2 and NJ-3-1 represent two novel species of . Based on phenotypic, chemotaxonomic and phylogenetic properties, strains Gai3-2 (=CGMCC 1.16080=JCM 33550) and NJ-3-1 (=CGMCC 1.16040=JCM 33552) represent two novel species of the genus , for which the name sp. nov. and sp. nov. are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004900DOI Listing
July 2021

Cinnamolide sesquiterpene lactone suppresses in vitro and in vivo cancer cell growth in cisplatin-resistant human cervical carcinoma cells by inducing mitochondrial mediated apoptosis, caspase activation, loss of MMP and targeting Akt/β-Catenin signaling pathway.

J BUON 2021 May-Jun;26(3):1187

Department of Obstetrics, Affiliated Hospital of Chengde Medical University, Chengde, Hebei 067000, China.

The Editors of JBUON issue an Expression of Concern to 'Cinnamolide sesquiterpene lactone suppresses in vitro and in vivo cancer cell growth in cisplatin-resistant human cervical carcinoma cells by inducing mitochondrial mediated apoptosis, caspase activation, loss of MMP and targeting Akt/β-Catenin signaling pathway', by Jing Hou, Changli Kan, Yanju Zhu, Yi Zhang, Bingfeng Zhou, Chunli Ren, Jiuyuan Fu, Yanwei Guo, Jinhuan Zhang; JBUON 2020;25(2):709-715; PMID: 32521857. Following the publication of the above article, readers drew to our attention that part of the data was possibly unreliable. We sent emails to the authors with a request to provide the raw data to prove the originality, but received no reply. Therefore, as we continue to work through the issues raised, we advise readers to interpret the information presented in the article with due caution. We thank the readers for bringing this matter to our attention. We apologize for any inconvenience it may cause.
View Article and Find Full Text PDF

Download full-text PDF

Source
July 2021

Molecular mechanisms underlying macrophage immunomodulatory activity of Rubus chingii Hu polysaccharides.

Int J Biol Macromol 2021 Aug 6;185:907-916. Epub 2021 Jul 6.

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, China. Electronic address:

The present study was to investigate the mechanisms involved in macrophage activation by polysaccharides from the fruits of Rubus chingii Hu (RFPs). The results showed that RFPs enhanced pinocytic and phagocytic activity, promoted the expression and secretion of inflammatory factors (ROS, PTGS2, iNOS, IL-6, IL-10 and TNF-α) and chemokines (CCL2 and CXCL10), and boosted the expression of accessory and costimulatory molecules (CD40, CD80, CD86, MHC-I and MHC-II). RNA-Seq analysis identified 2564 DEGs, 1710 GO terms and 101 KEGG pathways. TNF was identified as the core gene via analysis of pathway information integration and PPI network. The western blot analysis combined with functional verification assay confirmed that MAPK, NF-κB and Jak-STAT pathways were essential to RFPs-mediated macrophage activation. TLR2 was revealed to be the functional receptor and involved in the early recognition of RFPs. These results indicated that RFPs modulated macrophage immune response mainly through TLR2-dependent MAPK, NF-κB and Jak-STAT pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2021.07.024DOI Listing
August 2021

Bivalent recognition of histone marks by BARD1.

Trends Cell Biol 2021 Sep 29;31(9):703-704. Epub 2021 Jun 29.

Department of Oncology, Mayo Clinic, Rochester, MN 55905, USA. Electronic address:

BARD1 directs BRCA1 to DNA damage sites to facilitate homology recombination. Zhou and colleagues have now determined the cryo-electron microscopy (cryo-EM) structure of BARD1 bound to a nucleosome core particle with two marks: H2AK15ub and H4K20me0. The structure illustrates how bivalent recognition of both marks mediates highly specified recruitment of the BARD1-BRCA1 complex.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tcb.2021.06.005DOI Listing
September 2021

[Screening and quantitative analysis of nine illicit antiallergics in emulsion cosmetics by ultra-high performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry].

Se Pu 2020 Dec;38(12):1423-1430

Wuhan Institute for Food and Cosmetic Control, Wuhan 430014, China.

A rapid and accurate analysis method based on ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight high-resolution mass spectrometry (UPLC-Q-TOF-HRMS) was developed to screen and determine nine antiallergy drugs in emulsion cosmetics. First, a standard library of the target compounds was established. The library contained the TOF-MS information and secondary MS information such as retention time, ion addition mode, mass error, isotope distribution, mass-to-charge ratio of the parent ion, and fragment ion distribution. According to the European Union regulation (SANTE/11945/2015), the standard for the qualitative determination by HRMS was determined; that is, each compound was confirmed by two ions with a mass error below 5%, and the abundance ratio of the two ions was less than 30%. Second, the instrument conditions and sample pretreatment conditions for the determination of different compounds were optimized, and the influence of different levels of quantitative ions on the matrix effect was compared. The following observations were made:(1) the addition of 0.1% formic acid to the water phase improved the response of the chromatographic peaks; (2) among the various solvent amounts tested (4, 5, 6, 8 mL acetonitrile and 4, 5, 6, 8 mL methanol), 4 mL acetonitrile showed the best extraction efficiency; (3) PRiME HLB had a better purification effect than the other two purification columns (C18 and HLB solid-phase extraction cartridges), thus reducing the interference of impurities and ensuring good recovery of the target compounds; (4) the use of two pairs of secondary product ion quantification could significantly reduce the matrix effect of anti-allergic compounds and improve the quantification accuracy. Finally, based on the above findings, the experimental procedure was established. The lotion samples were first ultrasonically extracted with acetonitrile and purified on the PRiME HLB column. Chromatographic separation was performed on a Waters XBridge C18 column with gradient elution using 0.1% (v/v) formic acid in water and acetonitrile. Finally, the sequential window acquisition of all theoretical mass spectra (SWATH), which shows obvious advantages in continuous and high-throughput acquisition, was selected for MS data acquisition. The retention time, mass accuracy, isotope distribution, and fragment ion matching ratio were used for fast qualitative screening, while the peak areas of characteristic product ions were used for precise quantification. All the calibration curves showed good linearity (>0.99) within the tested ranges (5-100 μg/L) under the optimum conditions. The limits of quantification (LOQs) were in the range of 0.05-0.10 mg/kg. The recoveries were in the range of 65.3%-107% at three spiked levels (0.10, 0.20, and 0.60 mg/kg), with relative standard deviations (RSDs, =6) below 20%. Compared with the existing ion exchange column methods, the proposed "one-step" purification method based on PRiME HLB is simpler and more rapid, where the extraction solution is filtered directly after allowing it to pass through the column, without any subsequent washing and elution procedures. In addition, the LOQs of this method are lower than those of other LC-MS/MS methods, indicating that the proposed method has higher sensitivity. The application of SWATH data acquisition makes it possible to achieve quantification with two pairs of product ions, thus reducing the matrix effect and ensuring accuracy of the quantitative results. Therefore, the proposed method is less time-consuming and operationally convenient, and it can be used for the rapid screening and accurate quantification of antiallergics in lotion samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3724/SP.J.1123.2020.06017DOI Listing
December 2020

Total Flavonoids of Petals Release -Butyl Hydroperoxide-Induced Oxidative Stress in BRL-3A Cells.

Oxid Med Cell Longev 2021 5;2021:5453047. Epub 2021 Jun 5.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065 Sichuan, China.

Antioxidant and hepatoprotective activities in vitro of saffron petals were examined in this study for better utilizing saffron ( L.) biowaste. Using the DPPH and ABTS radical scavenging method, we compared the antioxidant activity and the content of total flavonoid extracts from petals (TFESP), stamens (TFESS), and both saffron petals and stamens (TFEMS). The results showed that the antioxidant capacity and the flavonoid content of TFESP were higher than those of TFESS and TFEMS. Then, the hepatoprotective activity of TFESP was determined, and the silymarin was used as a positive control. The main components of TFESP were analysed by ultrahigh performance liquid chromatography (UPLC) photodiode array (PDA)/mass spectrometry (MS) and nuclear magnetic resonance (NMR). The result showed that (1) TFESP could release oxidative liver injury induced by -butyl hydroperoxide (t-BHP). (2) TFESP could reduce the accumulation of reactive oxygen species (ROS); enhance the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH); and then improve the total antioxidant capacity (T-AOC) in BRL-3A cells. (3) TFESP could enhance the expression of B-cell lymphoma-2 () and decrease the expression of and ; increase the expression of Kelch-like ECH-associated protein-1 (), (), superoxide dismutase, and heme oxygenase 1 (); and downregulate inducible nitric oxide synthase (), interleukin-6 (), and nuclear factor kappa B-9 (). (4) The main hepatoprotective component of TFESP was identified as kaempferol-3-o-sophoroside. The mechanism may be that kaempferol-3-o-sophoroside can protect t-BHP-induced cell injury by regulating the expression of antioxidant, antiapoptotic, and anti-inflammatory genes. Thus, saffron petals are a potential hepatoprotective resource worthy of development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5453047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203408PMC
June 2021

Manganese-Catalyzed Achmatowicz Rearrangement Using Green Oxidant HO.

J Org Chem 2021 07 28;86(14):9563-9586. Epub 2021 Jun 28.

Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.

Oxidation reactions have been extensively studied in the context of the transformations of biomass-derived furans. However, in contrast to the vast literature on utilizing the stoichiometric oxidants, such as -CPBA and NBS, catalytic methods for the oxidative furan-recyclizations remain scarcely investigated. Given this, we report a means of manganese-catalyzed oxidations of furan with low loading, achieving the Achmatowicz rearrangement in the presence of hydrogen peroxide as an environmentally benign oxidant under mild conditions with wide functional group compatibility.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.joc.1c00858DOI Listing
July 2021

High-efficiency ultra-compact near-infrared supercontinuum generated in an ultrashort cavity configuration.

Opt Express 2021 Jun;29(12):19140-19146

We report a novel method to generate near-infrared supercontinuum (SC) in an ultrashort cavity configuration with only 11.5 m. With the continuous laser diode pump, a near-infrared SC with 26.8 W average output power and a spectrum ranging from 900 nm to 2000nm is demonstrated, and the laser diode pump to supercontinuum conversion efficiency is up to 60%. The spectral and power characteristics of the generated SC under different lengths of germanium-doped fiber (GDF) were carefully studied. This near-infrared SC generation method has the advantages of simple structure, low cost and good stability and also possesses the shortest fiber laser cavity length ever reported to the best of our knowledge.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.425948DOI Listing
June 2021

Non-propagation fast phase diverse phase retrieval for wavefront measurement with generalized FFT-based basis function.

Opt Express 2021 Jun;29(12):18817-18830

Phase retrieval is an attractive optical testing method with a simple experimental arrangement. The sampling grids wave propagation computation based on the FFT operations is usually involved in each iterative process for the classical phase retrieval model. In this paper, a novel non-propagation optimization phase retrieval technique with the FFT-based basis function is proposed to accelerate wavefront measurement. The sampling grids wave diffraction propagation computation is converted to matrix-vector products that have small dimensions to reduce the computational burden. The diffraction basis function based on generalized numerical orthogonal polynomial and two-step Fresnel propagation is deduced, which is suitable for the generally shaped pupil. This paper provides a universal non-propagation framework to accelerate phase retrieval which is applicable to the arbitrarily shaped wavefront measurement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.424793DOI Listing
June 2021

Visible-Light-Mediated Divergent Silylfunctionalization of Alkenes.

Org Lett 2021 Jul 21;23(13):5128-5132. Epub 2021 Jun 21.

College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

1,2-Silylfunctionalization of alkenes is an efficient way to construct highly functionalized silicon-containing compounds. However, examples of 1,2-silylfunctionalization of alkenes using readily available hydrosilanes are limited. Herein, we present a visible-light-mediated divergent 1,2-silylfunctionalization of alkenes using hydrosilane under ambient conditions. A series of β-alkoxy, β-alkylthio, β-hydroxy, and β-indolyl silanes was obtained in good to excellent yields. Moreover, vinylsilanes were successfully prepared in the absence of an additional nucleophile.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c01658DOI Listing
July 2021

Lactate Modulates Cellular Metabolism Through Histone Lactylation-Mediated Gene Expression in Non-Small Cell Lung Cancer.

Front Oncol 2021 2;11:647559. Epub 2021 Jun 2.

Department of Scientific Research Office, Affiliated Hospital of Qinghai University, Xining, China.

Lactate has been observed to fuel TCA cycle and is associated with cancer progression in human lung cancer, the leading cause of cancer deaths worldwide, but the effect of lactate on lung cancer metabolism is rarely reported. In this study, disordered metabolism in non-small cell lung cancer was demonstrated by increased G6PD and SDHA protein levels immunofluorescence, and up-regulated lactate dehydrogenase was found to be associated with poor prognosis. Then flow cytometry and Seahorse XFe analyzer were utilized to detect the effect of lactate on glycolysis and mitochondrial function in non-small cell lung cancer cells. The results show that in non-small cell lung cancer cells lactate attenuates glucose uptake and glycolysis while maintaining mitochondrial homeostasis as indicated by improved mitochondrial membrane potential. Further exploration found that mRNA levels of glycolytic enzymes (, ) and TCA cycle enzymes (, ) are respectively down-regulated and up-regulated by lactate, and increased histone lactylation was observed in promoters of and chromatin immunoprecipitation assay. Taken together, the above results indicate that lactate modulates cellular metabolism at least in part through histone lactylation-mediated gene expression in non-small cell lung cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.647559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208031PMC
June 2021

The nearly complete genome of Ginkgo biloba illuminates gymnosperm evolution.

Nat Plants 2021 06 14;7(6):748-756. Epub 2021 Jun 14.

The Southern Modern Forestry Collaborative Innovation Center, the Key Lab of Tree Genetics and Biotechnology of Educational Department of China and the Key Lab of Tree Genetics and Silvicultural Sciences of Jiangsu Province, Nanjing Forestry University, Nanjing, China.

Gymnosperms are a unique lineage of plants that currently lack a high-quality reference genome due to their large genome size and high repetitive sequence content. Here, we report a nearly complete genome assembly for Ginkgo biloba with a genome size of 9.87 Gb, an N50 contig size of 1.58 Mb and an N50 scaffold size of 775 Mb. We were able to accurately annotate 27,832 protein-coding genes in total, superseding the inaccurate annotation of 41,840 genes in a previous draft genome assembly. We found that expansion of the G. biloba genome, accompanied by the notable extension of introns, was mainly caused by the insertion of long terminal repeats rather than the recent occurrence of whole-genome duplication events, in contrast to the findings of a previous report. We also identified candidate genes in the central pair, intraflagellar transport and dynein protein families that are associated with the formation of the spermatophore flagellum, which has been lost in all seed plants except ginkgo and cycads. The newly obtained Ginkgo genome provides new insights into the evolution of the gymnosperm genome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41477-021-00933-xDOI Listing
June 2021

Vitrectomy With Silicone Oil Tamponade and Without Internal Limiting Membrane Peeling for the Treatment of Myopic Foveoschisis With High Risk of Macular Hole Development.

Front Med (Lausanne) 2021 28;8:648540. Epub 2021 May 28.

Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Department of Ophthalmology, Peking University People's Hospital, Beijing, China.

To explore the efficiency and safety of the surgical procedure of pars plana vitrectomy (PPV) with silicone oil (SO) tamponade and without internal limiting membrane (ILM) peeling for myopic foveoschisis (MF) eyes with high risk of macular hole formation. Three eyes (three patients) with MF and foveal detachment were enrolled into the study. Comprehensive preoperative ophthalmological assessments, including best corrected visual acuity (BCVA) and spectral-domain optical coherence tomography (SD-OCT) were performed on the eyes. Central foveal thickness (CFT) and thickness of continuous neurosensory retina at foveola were measured. All patients underwent PPV followed by SO tamponade and without ILM peeling. SO was removed when MF and retinal detachment were resolved. Patients were followed up postoperative at month 1, 3, 6, and 12. All the three eyes achieved complete resolution of MF and foveal reattachment with an average SO tamponade period of 11.67 ± 0.58 months. The average CFT at 6 months was 91 ± 27.5 μm, hence reduced significantly from baseline at 365.3 ± 137.85 μm ( = 0.037). There was no postoperative macular hole formation despite the average preoperative sensory retina thickness of 58 ± 20.07 μm. Mean BCVA was improved from logMAR 1.43 ± 0.75 to logMAR 0.8 ± 0.75 on the last follow-up. Manageable SO-related complications were reported, including SO emulsification, ocular hypertension, and cataract. Vitrectomy with SO tamponade and without ILM peeling as an optional surgical protocol to treat MF is effective and safe, especially for MF eyes vulnerable to macular hole formation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.648540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193352PMC
May 2021

Aux/IAA and ARF Gene Families in : Identification, Evolution, and Dynamic Transcriptome Profiling During the Plant Growth Process.

Front Plant Sci 2021 26;12:666310. Epub 2021 May 26.

Key Laboratory of Tree Genetics and Biotechnology of Educational Department of China, College of Forestry, Nanjing Forestry University, Nanjing, China.

The phytohormone auxin plays a pivotal role in the regulation of plant growth and development, including vascular differentiation and tree growth. The auxin/indole-3-acetic acid (Aux/IAA) and auxin response transcription factor (ARF) genes are key components of plant auxin signaling. To gain more insight into the regulation and functional features of Aux/IAA and ARF genes during these processes, we identified 38 AUX/IAA and 34 ARF genes in the genome of and characterized their gene structures, conserved domains, and encoded amino acid compositions. Phylogenetic analysis of some typical land plants showed that the Aux/IAA and ARF genes of Salicaceae originated from a common ancestor and were significantly amplified by the ancestral eudicot hexaploidization event and the "salicoid" duplication that occurred before the divergence of poplar and willow. By analyzing dynamic transcriptome profiling data, some Aux/IAA and ARF genes were found to be involved in the regulation of plant growth, especially in the initial plant growth process. Additionally, we found that the expression of several miR160/miR167-ARFs was in agreement with canonical miRNA-ARF interactions, suggesting that miRNAs were possibly involved in the regulation of the auxin signaling pathway and the plant growth process. In summary, this study comprehensively analyzed the sequence features, origin, and expansion of Aux/IAA and ARF genes, and the results provide useful information for further studies on the functional involvement of auxin signaling genes in the plant growth process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.666310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188177PMC
May 2021

MET amplification attenuates lung tumor response to immunotherapy by inhibiting STING.

Cancer Discov 2021 Jun 7. Epub 2021 Jun 7.

Hubei Cancer Hospital.

Immune checkpoint blockade (ICB) has revolutionized cancer therapy. However, the response of patients to ICB is difficult to predict. Here, we examined 81 lung cancer patients under ICB treatment and found that patients with MET amplification were resistant to ICB and had a poor progress-free survival. Tumors with MET amplifications had significantly decreased STING levels and antitumor T cell infiltration. Furthermore, we performed deep single-cell RNA sequencing on more than 20000 single immune cells and identified an immunosuppressive signature with increased subsets of XIST- and CD96-positive exhausted NK cells and decreased CD8+ T cell and NK cell populations in patients with MET-amplification. Mechanistically, we found that oncogenic MET signaling induces phosphorylation of UPF1 and downregulates tumor cell STING expression via modulation of the 3'-UTR length of STING by UPF1. Decreased efficiency of ICB by MET amplification can be overcome by inhibiting MET.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1158/2159-8290.CD-20-1500DOI Listing
June 2021

Smoothing process of conformal vibration polishing for mid-spatial frequency errors: characteristics research and guiding prediction.

Appl Opt 2021 May;60(13):3925-3935

By combining the conformal polishing method with short stroke vibration, a novel, to the best of our knowledge, conformal vibration polishing (CVP) method is proposed. The CVP method is expected to be an efficient means of optical processing by its high material removal rate and smoothing characteristics of mid-spatial frequency (MSF) errors. A quantitative time-domain smoothing model and a convergence factor (${\rm CF}_C$) are presented based on the research of smoothing characteristics. The motion mechanism, material removal ability, solution, and expansion of the smoothing model are demonstrated theoretically and experimentally. The experimental results exhibited good agreement with the theoretical predictions for the proposed method. The research provides a certain theoretical foundation for parameter selection and process optimization of the CVP method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/AO.424005DOI Listing
May 2021

Hsa Circ 001839 Promoted Inflammation in Renal Ischemia-Reperfusion Injury Through NLRP3 by miR-432-3p.

Nephron 2021 11;145(5):540-552. Epub 2021 May 11.

College of Sports, Southwest Medical University, Luzhou, China.

Background: In recent years, increasing discovery of the extremely important regulatory effects of circular RNAs on biological development, angiogenesis, tumor genesis, and development, as well as stem cell proliferation and differentiation has provided new opportunities for investigating regulation mechanism in angiogenesis.

Objectives: This study explored the expression of circ 001839 in renal ischemia-reperfusion injury (RI-RI) rats and whether its upstream microRNA-432-3p (miR-432-3p) affects inflammation in both RI-RI rats and NRK52E cells.

Methods: Rat model of RI-RI was made, and circ 001839 was identified by the gene-chip analysis in RI-RI rats. Expression of circ 001839 and miR-432-3p was measured by reverse transcription-quantitative polymerase chain reaction, protein expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, interferon (IFN)-γ, IL-6, and IL-18 in rat serum and cell supernatant was determined by ELISA, and the expression of NOD-like receptor 3 (NLRP3) and other gap-associated proteins in NRK52E cells was evaluated by Western blot analysis. Next, to verify the regulatory relationship between circ 001839 and miR-432-3p, 2 luciferase reporters were constructed.

Results: Circ 001839 expression of RI-RI rats and NRK52E cells was significantly upregulated, compared with the control group. Circ 001839 overexpression significantly increased inflammation through promoting TNF-α, IFN-γ, and IL-6 expression levels in NRK52E cells. Overexpression of miR-432-3p significantly promoted inflammation in NRK52E cells via induction of NLRP3. Moreover, miR-432-3p decreased the effects of circ 001839-induced inflammation in NRK52E cells.

Conclusions: These findings suggested that circ 001839 promoted inflammation in RI-RI through NLRP3 by miR-432-3p.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000515279DOI Listing
May 2021

Photoinduced Oxidative Alkoxycarbonylation of Alkenes with Alkyl Formates.

Org Lett 2021 May 11;23(10):3939-3943. Epub 2021 May 11.

College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

A photoinduced oxidative alkoxycarbonylation of alkenes initiated by intermolecular addition of alkoxycarbonyl radicals has been demonstrated. Employing alkyl formates as alkoxycarbonyl radical sources, a range of α,β-unsaturated esters were obtained with good regioselectivity and selectivity under ambient conditions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.orglett.1c01096DOI Listing
May 2021

Environmental robustness of the global yeast genetic interaction network.

Science 2021 05;372(6542)

The Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON M5S 3E1, Canada.

Phenotypes associated with genetic variants can be altered by interactions with other genetic variants (GxG), with the environment (GxE), or both (GxGxE). Yeast genetic interactions have been mapped on a global scale, but the environmental influence on the plasticity of genetic networks has not been examined systematically. To assess environmental rewiring of genetic networks, we examined 14 diverse conditions and scored 30,000 functionally representative yeast gene pairs for dynamic, differential interactions. Different conditions revealed novel differential interactions, which often uncovered functional connections between distantly related gene pairs. However, the majority of observed genetic interactions remained unchanged in different conditions, suggesting that the global yeast genetic interaction network is robust to environmental perturbation and captures the fundamental functional architecture of a eukaryotic cell.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1126/science.abf8424DOI Listing
May 2021

Prevalence of Childhood Asthma and Allergies and Their Associations with Perinatal Exposure to Home Environmental Factors: A Cross-Sectional Study in Tianjin, China.

Int J Environ Res Public Health 2021 04 14;18(8). Epub 2021 Apr 14.

Tianjin Key Laboratory of Indoor Air Environmental Quality Control, School of Environmental Science and Engineering, Tianjin University, No.135 Yaguan Road, Haihe Education Park, Tianjin 300350, China.

Asthma, rhinitis, and eczema are becoming increasingly prevalent among children in China. Studies have shown that the perinatal period is critical and impacts children's health. However, research on the associations between perinatal factors and childhood allergic diseases in China are few. We investigated 7366 children of ages 0-8 years old. The childhood asthma and allergies were surveyed by distributing questionnaires, modelled after Dampness in Buildings and Health (DBH) study in Sweden and had been validated. To determine the prevalence of the allergic conditions, explore for potential confounders, and analyze the associations between the allergies and the home environmental factors, chi-square test and binary logistic regression models (enter method) were employed. The prevalence of children's doctor-diagnosed asthma, rhinitis, and eczema were 4.4%, 9.5%, and 39.1%, respectively. After adjusting for sociodemographic factors, the negative effect of dampness/humidity on children's health became more obvious, with odd ratios (aORs) of up to 1.70 (95% confidence interval (CI): 1.12-2.57) for doctor-diagnosed asthma (DDA), 2.12 (95% CI: 1.38-3.25) for doctor-diagnosed rhinitis (DDR) and 1.79 (95% CI: 1.46-2.21) for doctor-diagnosed eczema (DDE). With parental smoking, aORs of up to 4.66 (95% CI: 1.99-10.92) for DDA and 1.74 (95%: 1.00-3.02) for DDE. Renovation exhibited aORs of up to 1.67 (95% CI: 1.13-2.47) for DDR. Although they showed no significant associations with some of the health outcomes, contact with animals, in general, were risk factors for the allergic conditions. Generally, the indoor environmental factors around the perinatal period were significant risk factors for the doctor-diagnosed allergic conditions discussed in this study.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph18084131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8070727PMC
April 2021

sp. nov. and sp. nov., isolated from saline soil and a salt mine.

Int J Syst Evol Microbiol 2021 Apr;71(4)

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.

A polyphasic study was undertaken to determine the taxonomic position of two halophilic archaeal strains, TH32 and YPL4, isolated from saline soil and a salt mine in PR China, respectively. Strains TH32 and YPL4 both have two dissimilar 16S rRNA genes. The two strains exhibited sequence similarities of 91.5-95.5 % for 16S rRNA genes and 90.9 % for the gene. Sequence similarities of 16S rRNA genes and the gene between the two strains and the current four members of were 90.6-97.4 % and 91.4-93.5 %, respectively. Phylogenetic analysis revealed that the two strains formed different branches separating them from the current members. Several phenotypic characteristics differentiate strains TH32 and YPL4 from current members. The polar lipids of the two strains are phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and four glycolipids. Two of the glycolipids are chromatographically identical to disulfated mannosyl glucosyl diether and sulfated mannosyl glucosyl diether, respectively, and the remaining two glycolipids are unidentified. The average nucleotide identity (ANI) and DNA-DNA hybridization (DDH) values between the two strains and the current members of (ANI 80.4-89.2 % and DDH 24.0-41.8 %) were much lower than the threshold values proposed as a species boundary, suggesting that the two strains represent novel species of . The values between the two strains (ANI 81.3 % and DDH 24.9 %) were also much lower than the recommended threshold values, which revealed that the two strains represent two genomically different species of . These results showed that strains TH32 (=CGMCC 1.15190=JCM 30840) and YPL4 (=CGMCC 1.15329=JCM 31108) represent two novel species of , for which the names sp. nov. and sp. nov. are proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004775DOI Listing
April 2021
-->