Publications by authors named "Jing He"

1,409 Publications

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Hypochlorous acid initiated lipid chlorination at air-water interface.

Sci Total Environ 2021 Jul 29;798:149320. Epub 2021 Jul 29.

School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China. Electronic address:

There has been a surge of interest in interfacial hypochlorous acid (HOCl) chemistry for indoor air quality and public health. Here we combined nanoelectrospray mass spectrometry (nESI-MS) and acoustic levitation (AL) techniques to study the chlorination chemistry of three model lipids (DPPE, POPG, DOPG) mediated by HOCl at the air-water interface of levitated water droplet. For DPPE with no CC double bonds, HOCl was insensitive to the alkane chains, and showed considerable delay directing to head amino groups compared to that in aqueous environment. Chlorination chemistry, for POPG and DOPG with CC double bonds, preferentially reacted with double bonds of one chain. The mechanism was discussed in light of these observations, and it is concluded that the increased hydrophilicity of the chlorinated chain disturbed the lipid packing and attracted it toward the water phase. In addition, the reaction rate constant and reactive uptake coefficient suggested that the chlorination of lipids exposed to HOCl at the air-water interface is likely to occur rapidly. These results gain the knowledge of HOCl mediated lipid interface reaction at the molecule level, and would better understand the adverse health effects associated with elevated indoor pollutants.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149320DOI Listing
July 2021

Predictors of Failed Intrauterine Balloon Tamponade in the Management of Severe Postpartum Hemorrhage.

Front Med (Lausanne) 2021 15;8:656422. Epub 2021 Jul 15.

Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, Zhengzhou, China.

To identify the factors predicting intrauterine balloon tamponade (IUBT) failure for severe postpartum hemorrhage (PPH) after delivery, we conducted a retrospective cohort study of women who underwent IUBT for severe PPH after delivery from October 1, 2016 until September 30, 2017. The failure of IUBT was defined as the need of additional surgical procedures or uterine embolization. A total of 99,650 deliveries occurred during the study period. Among the patients, 106 cases of severe PPH were managed with IUBT, and the global success rate was 70.8% (75/106). Least absolute shrinkage and selection operator (LASSO) regression was performed to select the potential risk factors predicting IUBT failure. The associated risk factors-obesity, multiple gestation, cesarean delivery, estimated blood loss (EBL), and placenta accreta spectrum (PAS)-were included in multivariate logistic models. Ultimately, these models identified multiple gestation, EBL, and PAS as independent risk factors for IUBT failure. In conclusion, IUBT is an effective method for severe PPH. The presence of factors affecting IUBT failure should be recognized early, and other modalities of management should be anticipated.
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http://dx.doi.org/10.3389/fmed.2021.656422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8319466PMC
July 2021

Sustained attention deficits in adults with juvenile-onset type 1 diabetes mellitus.

Psychosom Med 2021 Jul 23. Epub 2021 Jul 23.

Medical Psychological Center, The Second Xiangya Hospital, Central South University, Changsha, China Medical Psychological Institute of Central South University, Changsha, China National Clinical Research Center for Mental Disorders, The Second Xiangya Hospital, Changsha, China Department of Psychology, Hunan First Normal University, Changsha, China Hunan Key Laboratory of Children's Psychological Development and Brain Cognitive Science, Hunan First Normal University, Changsha, China National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology (Central South University), Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, China. Xiangya School of Nursing, Central South University, Changsha, Hunan Province, China. Department of Nutrition, The Second Xiangya Hospital, Central South University, National Clinical Research Center for Metabolic Diseases, Changsha, China.

Objective: This study aimed to investigate whether patients with juvenile-onset type 1 diabetes mellitus (T1DM) have poorer sustained attention than their counterparts with adult-onset T1DM, and whether there is a relationship between diabetes-related variables and sustained attention.

Methods: This study included 76, 68, and 85 participants with juvenile-onset and adult-onset T1DM, and healthy controls (HCs), respectively. All participants completed the Sustained Attention to Response Task, Beck Depression Inventory-II, and the Chinese version of the Wechsler Adult Intelligence Scale.

Results: The juvenile-onset group showed more omission errors (p = .007) than the adult-onset group and shorter reaction time (p = .005) than HCs, while the adult-onset group showed no significant differences compared with HCs. Hierarchical linear regression analysis revealed that the age of onset was associated with omission errors in T1DM participants (β = -0.275, t = -2.002, p = .047). In the juvenile-onset group, the omission error rate were associated with the history of severe hypoglycemia (β = 0.225, t = 1.996, p = .050), while reaction time was associated with the age of onset (β = -0.251, t = -2.271, p = .026). Fasting blood glucose levels were significantly associated with reaction time in both the juvenile-onset and adult-onset groups (β = -0.236, t = -2.117, p = .038 and β = 0.259, t = 2.041, p = .046, respectively).

Conclusion: Adults with juvenile-onset T1DM have sustained attention deficits, in contrast to their adult-onset counterparts, suggesting that the disease adversely affects the developing brain. Both the history of severe hypoglycemia and fasting blood glucose levels are factors associated with sustained attention impairment. Early diagnosis and treatment in juvenile patients are required to prevent the detrimental effects of diabetes.
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http://dx.doi.org/10.1097/PSY.0000000000000992DOI Listing
July 2021

Decellularized tendon matrix membranes prevent post-surgical tendon adhesion and promote functional repair.

Acta Biomater 2021 Jul 23. Epub 2021 Jul 23.

Department of Tissue Engineering, China Medical University, Shenyang, China; Department of Developmental Cell Biology, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, China; NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial, Institute of Regulatory Science for Medical Device, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, 610064, China. Electronic address:

Adhesion often occurs after tendon injury, and results in sliding disorder and movement limitation with no ideal solution for it in clinic. In this study, an anti-adhesion membrane, i.e., decellularized tendon matrix (DTM) for tendon is successfully prepared by an optimized tendon decellularization method from homologous extracellular matrix. Microsection technology has been used to optimize the method of decellularization in order to better preserve the bioactive components in tissues and reduce the chemical reagent residues on the premise of effective decellularization with relatively shorter time and less reagents for decellularization. The physic-chemical properties and biological functions of DTM are evaluated, and high-throughput and high-precision tandem mass tags (TMT) labeling proteomics technology is used to analyze protein components of DTM, which may provide the scientific support for application of the innovative product. In vitro biosafety tests show that DTM not only is non-toxic but also promote cell proliferation. Subcutaneous implantation test confirms that DTM is completely degraded after 12 weeks and there is no obvious inflammatory reaction. The results of Achilles tendon repair in rabbits show that DTM can not only prevent tendon adhesion but also improve the quality of tendon repair, which demonstrates its tremendous application potential. STATEMENT OF SIGNIFICANCE: There is no ideal solution for adhesion after tendon injury. In this study, a dense tendon anti-adhesion membrane (DTM) was successfully prepared from homologous extracellular matrix (ECM). This DTM could effectively retain bioactive ingredients, and prevent adhesion as well as improve the quality of tendon repair in vivo. An optimized decellularization method was used which could effectively decellularize tendon in a short time, better preserve bioactive components, and reduce reagent residues. For the first time, high-throughput and high-precision tandem mass tags (TMT) labeling proteomics technology was used to qualitatively and quantitatively analyze the protein composition of fresh tendon, acellular tendon and DTM, which provided not only scientific support for the application of DTM, but also comprehensive and accurate data support for related research of bovine tendons and decellularization.
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http://dx.doi.org/10.1016/j.actbio.2021.07.038DOI Listing
July 2021

Dysphagia Secondary to Intraluminal Choriocarcinoma of the Gastroesophageal Junction.

Am J Gastroenterol 2021 Jul 15. Epub 2021 Jul 15.

Division of Thoracic Surgery, Henry Ford Health System, Detroit, Michigan, USA.

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http://dx.doi.org/10.14309/ajg.0000000000001379DOI Listing
July 2021

Systemic inhibition of PTPN22 augments anticancer immunity.

J Clin Invest 2021 Jul 20. Epub 2021 Jul 20.

Sidney Kimmel Comprehensive Cancer Center, John Hopkins University, Baltimore, United States of America.

Both epidemiologic and cellular studies in the context of autoimmune diseases have established that protein tyrosine phosphatase non-receptor type 22 (PTPN22) is a key regulator of T cell receptor (TCR) signaling. However, its mechanism of action in tumors and its translatability as a target for cancer immunotherapy have not been established. Here we show that a germline variant of PTPN22, rs2476601, portended a lower likelihood of cancer in patients. PTPN22 expression was also associated with markers of immune regulation in multiple cancer types. In mice, lack of PTPN22 augmented antitumor activity with greater infiltration and activation of macrophages, natural killer (NK) cells, and T cells. Notably, we generated a novel small molecule inhibitor of PTPN22, named L-1, that phenocopied the antitumor effects seen in genotypic PTPN22 knockout. PTPN22 inhibition promoted activation of CD8+ T cells and macrophage subpopulations toward MHC-II expressing M1-like phenotypes, both of which were necessary for successful antitumor efficacy. Increased PD1-PDL1 axis in the setting of PTPN22 inhibition could be further leveraged with PD1 inhibition to augment antitumor effects. Similarly, cancer patients with the rs2476601 variant responded significantly better to checkpoint inhibitor immunotherapy. Our findings suggest that PTPN22 is a druggable systemic target for cancer immunotherapy.
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http://dx.doi.org/10.1172/JCI146950DOI Listing
July 2021

Metagenomic Sequencing Analysis of the Effects of Colistin Sulfate on the Pig Gut Microbiome.

Front Vet Sci 2021 2;8:663820. Epub 2021 Jul 2.

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China.

The gut microbiome plays important roles in maintaining host health, and inappropriate use of antibiotics can cause imbalance, which may contribute to serious disease. However, despite its promise, using metagenomic sequencing to explore the effects of colistin on gut microbiome composition in pig has not been reported. Herein, we evaluated the roles of colistin in gut microbiome modulation in pigs. Metagenomic analysis demonstrated that overall microbial diversity was higher in the colistin group compared with the control group. Antibiotic Resistance Genes Database analysis demonstrated that following colistin treatment, expression levels of , and were significantly upregulated, indicating that colistin may induce transformation of antibiotic resistance genes. Colistin also affected the microbiome distribution patterns at both genus and phylum levels. In addition, at the species level, colistin significantly reduced the abundance of , and and enhanced the abundance of and compared to the control group. Gene Ontology analysis demonstrated that following treatment with colistin, metabolic process, cellular process, and single-organism process were the dominant affected terms. Kyoto Encyclopedia of Genes and Genomes analysis showed that oxidative phosphorylation, protein processing in endoplasmic reticulum, various types of N-glycan biosynthesis, protein processing in endoplasmic reticulum, pathogenic infection, and mitogen-activated protein kinase signaling pathway-yeast were the dominant signaling pathways in the colistin group. Overall, our results suggested that colistin affects microbial diversity and may modulate gut microbiome composition in pig, potentially providing novel strategy or antibiotic rationalization pertinent to human and animal health.
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http://dx.doi.org/10.3389/fvets.2021.663820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282896PMC
July 2021

Soluble ST2 and mixed venous oxygen saturation for prediction of mortality in patients with pulmonary hypertension.

J Thorac Dis 2021 Jun;13(6):3478-3488

Department of Cardio-Pulmonary Circulation, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China.

Background: Although soluble suppression of tumorigenicity-2 (sST2) has been identified as a clinical biomarker for pulmonary hypertension (PH) by previous studies, the implication of sST2 combined with hemodynamic parameters in PH has not been well studied. This study aimed to evaluate the relationship between sST2 and hemodynamic parameters and to evaluate the predictive value of sST2 for mortality in patients with PH.

Methods: One hundred eighty-four incident patients with PH and 14 healthy controls were retrospectively enrolled by Shanghai Pulmonary Hospital for this retrospective study. After all patients underwent right heart catheterization, blood samples were collected and serum sST2 concentration was assessed by the Presage™ ST2 assay. Kaplan-Meier curve and Cox regression analyses were used to predict survival and the association between survival and different factors such as sST2, SvO.

Results: During a follow-up of 44.9 (IQR 28.5-64.4) months, 65 patients died. The median concentration of sST2 in PH patients was 33.1 ng/mL, which is higher than that in control group (23.1 ng/mL, P=0.005). Furthermore, for PH group, the level of sST2 was higher in non-survivors than that in survivors. Cox regression analyses demonstrated that sST2 and SvO were independent risk factors for survival. In Kaplan-Meier curve analyses, elevated sST2 level and reduced SvO predicted a poor outcome for patients with PH.

Conclusions: Higher sST2 was independently associated with increased mortality, as well as lower SvO in patients with PH. Especially, the combination of higher sST2 and lower SvO had the strongest predictive value of mortality in patients with PH.
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http://dx.doi.org/10.21037/jtd-20-2732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264676PMC
June 2021

Survival in severe pulmonary hypertension due to chronic lung disease: influence of in-hospital platelet distribution width.

Pulm Circ 2021 Jul-Sep;11(3):20458940211026484. Epub 2021 Jun 30.

Department of Cardio-Pulmonary Circulation, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Platelet distribution width has been recognized as risk predictors of idiopathic pulmonary arterial hypertension. This study aims to investigate whether in-hospital platelet distribution width would be useful to predict all-cause death in patients with severe pulmonary hypertension due to chronic lung diseases (CLD-PH). Early in-hospital platelet distribution width was measured in 67 severe CLD-PH patients who were confirmed by right heart catheterization and followed up. Event-free survival was estimated using the Kaplan-Meier method and analyzed with the log-rank test. Cox proportional hazards models were performed to determine the association between the platelet distribution width level and all-cause death. During median of 2.4 (2.5, 3.7) years of follow-up, 44 patients died. A significant association was noted between in-hospital platelet distribution width level and the adjusted risk of all-cause mortality (hazard ratio: 1.245; 95% confidence interval: 1.117-1.386,  < 0.001). Compared with those with platelet distribution width <16.1%, the hazard ratio for all-cause death increased by 5.278 (95% confidence interval: 2.711-10.276,  < 0.0001) among patients with platelet distribution width ≥16.1%. Higher levels of platelet distribution width were also associated with increased risk of all-cause death. In-hospital platelet distribution width was independently associated with all-cause death in patients with severe CLD-PH. This potentially could be used to estimate the severity of severe CLD-PH.
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http://dx.doi.org/10.1177/20458940211026484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258768PMC
June 2021

Protective effect of Phaeoporus obliquus polysaccharide against acute liver injury induced by carbon tetrachloride and alcohol in mice.

Pak J Pharm Sci 2021 Mar;34(2):649-656

College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, PR China.

Studied the optimum extraction process of polysaccharide from Phaeoporus obliquus and the effect of Phaeoporus obliquus polysaccharide on carbon tetrachloride (CCl)- or alcohol-induced acute liver injury in mice. The main factor in influencing the extraction rate of Phaeoporus obliquus polysaccharide were extraction power and time, which was a kind of pyran glucose by infrared spectroscopy. CCl and alcohol were employed respectively to establish CCl4 and alcohol-induced acute liver injury mouse models. Compared with model groups mice, Phaeoporus obliquus polysaccharide treatment at the doses of 100mg/kg and 200mg/kg exhibited an obvious reduction liver index, ALP, ALT, AST levels, MDA content and TNF-α level (p<0.01) and SOD activity was increased, which was in a dose-dependent manner. Compared with the model group, the necrosis degree of hepatocytes was obviously reduced and the small fat droplets were formed in some cytoplasm, especially in high dose group, which the liver cells recovered to the level of normal group. Rt-PCR results showed that the expression of CYP2E1 mRNA in liver tissues of Phaeoporus obliquus polysaccharide groups were significantly reduced, and the difference were statistically significant compared with the model group (p<0.05). These results demonstrated that Phaeoporus obliquus polysaccharide has significantly hepatoprotective effect on CCl4 and alcohol-induced acute liver injury in mice.
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March 2021

Polymorphisms in METTL3 gene and hepatoblastoma risk in Chinese children: A seven-center case-control study.

Gene 2021 Jul 16;800:145834. Epub 2021 Jul 16.

Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, Guangdong, China. Electronic address:

Hepatoblastoma is the most common malignant liver cancer in childhood, yet its etiology remains unclear. As an mA methylation modifier, methyltransferase like 3 (METTL3) has an active methyltransferase domain that functionally participates in various tumor occurrence and development. However, little is known about how METTL3 polymorphisms affect the occurrence of hepatoblastoma. Here, we attempted to investigate the associations between METTL3 gene polymorphisms and hepatoblastoma risk in a seven-center case-control study. We genotyped four METTL3 polymorphisms (rs1061026 T > G, rs1061027 C > A, rs1139130 A > G, rs1263801 G > C) by TaqMan technique in 313 cases and 1446 controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate the contributions of these four single nucleotide polymorphisms (SNPs) to hepatoblastoma susceptibility. In single genotype analysis, we detected no significant correlation between these four polymorphisms in METTL3 and hepatoblastoma risk. However, in the combined analysis, the presence of 2-4 risk genotypes of METTL3 was associated with an increased risk of hepatoblastoma compared with that of 0-1 risk genotypes (adjusted OR = 1.48, 95% CI = 1.03-2.12, P = 0.035). The stratified analysis further revealed that carriers of 2-4 risk genotypes are more susceptible to hepatoblastoma in the subgroups of subjects aged under 17 months (adjusted OR = 1.88, 95% CI = 1.12-3.16, P = 0.016) and females (adjusted OR = 1.79, 95% CI = 1.06-3.05, P = 0.031). Overall, our results revealed that none of these four SNPs could increase susceptibility to hepatoblastoma individually. Carriers with 2-4 risk genotypes in the combined analysis tend to increase the risk of hepatoblastoma.
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http://dx.doi.org/10.1016/j.gene.2021.145834DOI Listing
July 2021

Prognosis and Chemotherapy Use in Breast Cancer Patients with Multiple Lymphatic Micrometastases: An NCDB Analysis.

Ann Surg Oncol 2021 Jul 11. Epub 2021 Jul 11.

Department of Surgery, University of Texas Medical Branch, Galveston, TX, USA.

Background: The number of involved lymph nodes negatively affects prognosis in breast cancer patients. Nevertheless, current staging and treatment recommendations do not distinguish between patients with single versus multiple lymphatic micrometastases. In this study, we aim to better characterize these patients.

Methods: The National Cancer Database was retrospectively queried to identify 486,800 women with stage I-III, estrogen receptor-positive/progesterone receptor-positive/human epidermal growth factor receptor 2-negative (ER+/PR+/HER2-) breast cancer and nodal status of N0, N1mi with 1 (Nmic1) or more (Nmic > 1) involved nodes, and N1 with 1 involved node (N1.1), from 2010 to 2016. Patients with different nodal statuses were compared regarding treatment characteristics, survival, and benefit from chemotherapy by their 21-gene recurrence score (RS).

Results: Of the 23,072 N1mi patients, 88.3% were Nmic1 and 11.7% were Nmic > 1. Nmic > 1 patients were younger, had larger and higher-grade tumors, with more lymphovascular invasion, and were more commonly treated by axillary dissection, radiation, and chemotherapy than Nmic1 patients. In that, they were comparable with N1.1 patients. Five-year survival of Nmic > 1 patients (88.1%) was worse than Nmic1 patients (90.1%; p = 0.02), but similar to N1.1 patients (87.9%). Nmic1, Nmic > 1, and N1.1 patients with RS 11-25 exhibited a < 2% absolute survival benefit associated with chemotherapy. With RS > 25, Nmic > 1 patients showed a 3.5% benefit, similar to Nmic1 (4.8%) and lower than N1.1 (10.9%) patients.

Conclusions: Nmic > 1 breast cancer patients have worse prognoses than Nmic1 patients, similar to N1.1 patients. Our data suggest those patients with RS 11-25 have minimal benefit from chemotherapy. These findings should be taken into account when discussing prognosis and considering chemotherapy in patients with lymphatic micrometastases.
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http://dx.doi.org/10.1245/s10434-021-10374-7DOI Listing
July 2021

Elevated Serum Human Epididymis Protein 4 Is Associated With Disease Activity and Systemic Involvements in Primary Sjögren's Syndrome.

Front Immunol 2021 23;12:670642. Epub 2021 Jun 23.

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.

Background: We aimed to investigate the clinical utility of human epididymis protein 4, a tumor biomarker being widely utilized in clinical practice in the diagnosis of ovarian cancer, in primary Sjögren's Syndrome (pSS).

Methods: A total of 109 pSS patients and 113 healthy controls (HCs) were included in the study. HE4 were determined by Roche Cobas E601 electrochemical luminescence analyzer. Clinical and laboratory findings were reviewed, and the relationships between HE4 and clinical parameters were determined by Spearman's correlation test. The European league against rheumatism Sjögren's syndrome disease activity index (ESSDAI) was utilized to evaluate disease activity.

Findings: The levels of HE4 were significantly elevated in patients with pSS compared to HCs (103.65 pmol/L 46.52 pmol/L, <0.001). The levels of HE4 were positively correlated with ESSDAI scores (=0.462, <0.001). Significant positive correlations between the levels of HE4 with pulmonary involvements (=0.442, <0.001) and renal involvements (=0.320, =0.001) were observed. Receiver operating curve (ROC) analysis revealed an optimal cut-off value of 104.90 pmol/L and 128.05 pmol/L for distinguishing patients with pulmonary and renal involvements, with the areas under the ROC curve (AUCs) of 0.778 (95%CI 0.685-0.870, <0.001) and 0.768 (95%CI 0.646-0.891, =0.001), respectively. Among patients with pulmonary involvement, the levels of HE4 were positively correlated with the semiquantitative HRCT grade (=0.417, =0.016), and negatively correlated with the percentage of forced vital capacity (FVC) (= -0.460, =0.047) and diffusing capacity of the lung for carbon monoxide (DLco) (= -0.623, =0.004). For patients with renal involvement, HE4 was positively correlated with creatinine (=0.588, =0.021) and negatively correlated with estimated glomerular filtration rate (= -0.599, =0.030).

Conclusions: Our findings demonstrated a novel role of HE4 in clinical stratification of pSS, suggesting that introducing HE4 to the current pSS test panel may provide additional diagnostic value, particularly in evaluating disease activity and pulmonary/renal involvements.
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http://dx.doi.org/10.3389/fimmu.2021.670642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260678PMC
June 2021

Competition between spin density wave and charge density wave driven by interactions of spinful Haldane model on honeycomb lattices.

J Phys Condens Matter 2021 Jul 26;33(39). Epub 2021 Jul 26.

School of Sciences, Xi'an Technological University, Xi'an 710032, People's Republic of China.

We investigate the interacting spinful Haldane model with on-site and nearest-neighbor repulsions on honeycomb lattices. Interactions drive spin-density and charge-density waves competing with each other. This competition enriches the phase diagram of the system greatly, where both topological and non-topological coexistence phases emerge with spin-density and charge-density wave orders. Moreover, the topological phase transition accompanied by the energy gap closure in coexistence phases is of third order. Finally, we study the topological properties consisting ofnumbers and edge states for different phases.
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http://dx.doi.org/10.1088/1361-648X/ac1300DOI Listing
July 2021

Observation on the effect of modified adhesive retained posterior implant denture on the tissue around the implant.

Panminerva Med 2021 Jul 9. Epub 2021 Jul 9.

Center of Implant Dentistry, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, Shenyang, China -

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http://dx.doi.org/10.23736/S0031-0808.21.04368-8DOI Listing
July 2021

High glucose induces trafficking of prorenin receptor and stimulates profibrotic factors in the collecting duct.

Sci Rep 2021 Jul 5;11(1):13815. Epub 2021 Jul 5.

Department of Physiology, School of Medicine, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA, 70112, USA.

Growing evidence indicates that prorenin receptor (PRR) is upregulated in collecting duct (CD) of diabetic kidney. Prorenin is secreted by the principal CD cells, and is the natural ligand of the PRR. PRR activation stimulates fibrotic factors, including fibronectin, collagen, and transforming growth factor-β (TGF-β) contributing to tubular fibrosis. However, whether high glucose (HG) contributes to this effect is unknown. We tested the hypothesis that HG increases the abundance of PRR at the plasma membrane of the CD cells, thus contributing to the stimulation of downstream fibrotic factors, including TGF-β, collagen I, and fibronectin. We used streptozotocin (STZ) male Sprague-Dawley rats to induce hyperglycemia for 7 days. At the end of the study, STZ-induced rats showed increased prorenin, renin, and angiotensin (Ang) II in the renal inner medulla and urine, along with augmented downstream fibrotic factors TGF-β, collagen I, and fibronectin. STZ rats showed upregulation of PRR in the renal medulla and preferential distribution of PRR on the apical aspect of the CD cells. Cultured CD M-1 cells treated with HG (25 mM for 1 h) showed increased PRR in plasma membrane fractions compared to cells treated with normal glucose (5 mM). Increased apical PRR was accompanied by upregulation of TGF-β, collagen I, and fibronectin, while PRR knockdown prevented these effects. Fluorescence resonance energy transfer experiments in M-1 cells demonstrated augmented prorenin activity during HG conditions. The data indicate HG stimulates profibrotic factors by inducing PRR translocation to the plasma membrane in CD cells, which in perspective, might be a novel mechanism underlying the development of tubulointerstitial fibrosis in diabetes mellitus.
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http://dx.doi.org/10.1038/s41598-021-93296-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257763PMC
July 2021

Association of Total Bilirubin With All-Cause and Cardiovascular Mortality in the General Population.

Front Cardiovasc Med 2021 18;8:670768. Epub 2021 Jun 18.

Department of Cardiology, Affiliated Hospital of Nantong University, Nantong, China.

The study aims to investigate the association of total bilirubin with all-cause and cause-specific mortality in the general population. A total of 37,234 adults from the United States National Health and Nutrition Examination Survey 1999-2014 were enrolled. Baseline levels of total bilirubin associated with risk of mortality were evaluated on a continuous scale (restricted cubic splines) and by quartile categories with Cox regression models. Higher levels of total bilirubin was positively associated with an increased risk of all-cause mortality [hazard ratio (HR) 1.59, 95% confidence interval (CI) 1.46-1.72; < 0.001]. Compared with individuals with the lowest quartile of bilirubin, the multivariable adjusted hazard ratio for all-cause mortality was 1.25 (1.14-1.37) for individuals in the highest quartile. Restricted cubic splines indicated that the association was non-linear in cardiovascular mortality and cancer mortality while linear in all-cause mortality. Total bilirubin was associated with all-cause and cause-specific mortality in the general population.
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http://dx.doi.org/10.3389/fcvm.2021.670768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253250PMC
June 2021

Comparative Effectiveness of Exercise Training for Patients With Chronic Thromboembolic Pulmonary Hypertension After Pulmonary Endarterectomy: A Systematic Review and Meta-Analysis.

Front Cardiovasc Med 2021 17;8:664984. Epub 2021 Jun 17.

Department of Cardio-Pulmonary Circulation, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Patients with chronic thromboembolic pulmonary hypertension (CTEPH) still experience reduced exercise capacity despite pulmonary endarterectomy (PEA). Exercise training improves the exercise capacity and quality of life (QoL) in patients with PH, but data on the effects of exercise training on these patients are scarce. The aim of this meta-analysis and systematic review was to evaluate the effectiveness and safety of exercise training in CTEPH after PEA. We searched the relevant literature published before January 2020 for the systematic review and meta-analysis using the PubMed, EMBASE, and Cochrane Library databases. The primary outcome was a change in the 6-min walking distance (6 MWD). We also assessed the effect of exercise on the peak oxygen uptake (VO) or peak VO/kg, oxygen uptake anaerobic threshold, workload, oxygen pulse, hemodynamics, arterial blood gases, oxygen saturation, N-terminal pro-brain-type natriuretic peptide (NT-proBNP), quality of life (QoL) and pulmonary function tests. We included 4 studies with 208 exercise-training participants. In the pooled analysis, short-term exercise training can improve the 6 MWD of 58.89 m (95% CI: 46.26-71.52 m, < 0.0001). There was a significant increase in the peak VO/kg or peak VO after exercise training (3.15 ml/min/kg, 95% CI: 0.82-5.48, = 0.008; 292.69 ml/min, 95% CI: 24.62-560.75, = 0.032, respectively). After exercise training, the maximal workload and O pulse significantly improved. Three months of exercise training increased the right ventricular ejection fraction by 3.53% (95% CI: 6.31-11.94, < 0.00001, = 0) independently of PEA surgery. In addition, NT-proBNP plasma levels significantly improved with exercise training after PEA [weighted mean difference (WMD): -524.79 ng/L, 95% CI: 705.16 to -344.42, < 0.0001, = 0]. The partial pressure of oxygen and pH improved progressively over 12 weeks of exercise training (WMD: 4 mmHg, 95% CI: 1.01-8.33, = 0.01; WMD: 0.03, 95% CI: 0.02-0.04, < 0.0001, respectively). Subscales of the QoL measured by the SF-36 questionnaire had also improved. In addition, exercise training was well-tolerated with a low dropout rate, and no major adverse events occurred during exercise training. Exercise training may be associated with a significant improvement in the exercise capacity and QoL among CTEPH patients after PEA and was proven to be safe. However, more large-scale multicentre studies are needed to confirm the effectiveness and safety of exercise training in CTEPH patients after PEA. CRD42021235275.
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http://dx.doi.org/10.3389/fcvm.2021.664984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245692PMC
June 2021

[Determination of organophosphorus flame retardants in textile wastewater by dispersive liquid-liquid microextraction based on solidification of floating organic drop followed by ultra-high performance liquid chromatography-tandem mass spectrometry].

Se Pu 2020 Jun;38(6):679-686

College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.

A rapid method for the determination of five organophosphorus flame retardants (OPFRs) in textile wastewater was established by dispersive liquid-liquid microextraction (DLLME) based on solidification of floating organic drop (SFO) coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The analytes were extracted from the water samples by SFO-DLLME, which was performed using a mixture of an extraction solvent that was less dense than water, 1-undecanol, and a dispersive solvent, methanol. The influences of the SFO-DLLME parameters on the extraction efficiencies were studied. 1-Undecanol (extraction solvent, 400 μL) and methanol (dispersive solvent, 300 μL) were added to textile wastewater (containing 2 g NaCl) with pH between 6 and 9, and the solution was shaken for 2 min. Under optimum conditions, the linear ranges of the proposed method were from 2 μg/L to 100 μg/L with correlation coefficients () above 0.99 for all the analytes. The limits of detection (=3) ranged from 2 μg/L to 5 μg/L. The precision of the method was evaluated in terms of repeatability; the relative standard deviations varied from 2.7% to 11.2% (=6). The relative recoveries ranged from 71.6% to 117.6% for all analytes. Only 3 of the 11 selected samples were tested positive for OPFRs, and the total concentrations of OPFRs in them were in the range of 2.6-3.4 μg/L. Hence, this method is accurate, environmentally friendly, fast, and convenient for the routine analysis of OPFRs in textile wastewater.
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http://dx.doi.org/10.3724/SP.J.1123.2019.10027DOI Listing
June 2020

Genetic variations in nucleotide excision repair pathway genes and hepatoblastoma susceptibility.

Int J Cancer 2021 Jul 1. Epub 2021 Jul 1.

Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.

The etiology of hepatoblastoma is largely unknown due to the rarity of this disease. Nucleotide excision repair (NER), a versatile system in repairing DNA damage, is highly implicated in carcinogenesis. However, it remains unclear whether single nucleotide polymorphisms (SNPs) of genes in the NER pathway are related to hepatoblastoma risk. A total of 313 Chinese children diagnosed with hepatoblastoma and 1446 controls were recruited from seven hospitals across China. TaqMan assay was adopted to genotype 19 SNPs in NER pathway genes including ERCC1, XPA, XPC, XPD, XPF and XPG. Of them, only two SNPs in XPC gene predisposed to hepatoblastoma risk. The XPC rs2607775 polymorphism significantly contributed to hepatoblastoma risk (dominant model: adjusted OR = 1.44, 95% CI = 1.01-2.05, P = .046). However, XPC rs1870134 conferred a significantly decreased risk of hepatoblastoma in recessive model (adjusted OR = 0.50, 95% CI = 0.26-0.98, P = .042). Stratified analysis revealed that rs2607775 CG/GG genotype, rs1870134 CC genotype and four to five risk genotypes were associated with the risk of hepatoblastoma under certain subgroups. The significant relationships were confirmed by haplotype analyses and false-positive report probability analyses. In addition, expression quantitative trait locus analysis suggested that rs2607775 G increased expression of XPC mRNA. Collectively, our discover a promising candidate XPC gene as a biomarker for the risk of hepatoblastoma.
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http://dx.doi.org/10.1002/ijc.33722DOI Listing
July 2021

Comprehensive analysis of DNA methylation and gene expression profiles in gestational diabetes mellitus.

Medicine (Baltimore) 2021 Jul;100(26):e26497

Department of Obstetrics and Gynecology, The 2nd Affiliated Hospital of Wenzhou Medical University, Zhejiang, P. R. China.

Abstract: Gestational diabetes mellitus (GDM) has a high prevalence during pregnancy. This research aims to identify genes and their pathways related to GDM by combining bioinformatics analysis.The DNA methylation and gene expression profiles data set was obtained from Gene Expression Omnibus. Differentially expressed genes (DEG) and differentially methylated genes (DMG) were screened by R package limma. The methylation-regulated differentially expressed genes (MeDEGs) were obtained by overlapping the DEGs and DMGs. A protein-protein interaction network was constructed using the search tool for searching interacting genes. The results are visualized in Cytoscape. Disease-related miRNAs and pathways were retrieved from Human MicroRNA Disease Database and Comparative Toxic Genome Database. Real-time quantitative PCR further verified the expression changes of these genes in GDM tissues and normal tissues.After overlapping DEGs and DMGs, 138 MeDEGs were identified. These genes were mainly enriched in the biological processes of the "immune response," "defense response," and "response to wounding." Pathway enrichment shows that these genes are involved in "Antigen processing and presentation," "Graft-versus-host disease," "Type I diabetes mellitus," and "Allograft rejection." Six mRNAs (including superoxide dismutase 2 (SOD2), mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), dual specificity phosphatase 5 (DUSP5), p21-activated kinases 2 (PAK2), serine protease inhibitor clade E member 1 (SERPINE1), and protein phosphatase 1 regulatory subunit 15B (PPP1R15B)) were identified as being related to GDM. The results obtained by real-time quantitative PCR are consistent with the results of the microarray analysis.This study identified new types of MeDEGs and discovered their related pathways and functions in GDM, which may be used as molecular targets and diagnostic biomarkers for the precise diagnosis and treatment of GDM.
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http://dx.doi.org/10.1097/MD.0000000000026497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257864PMC
July 2021

MKP-1 is required to limit myeloid-cell mediated oral squamous cell carcinoma progression and regional extension.

Oral Oncol 2021 Jun 26;120:105401. Epub 2021 Jun 26.

Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA; Department of Head and Neck/Plastic and Reconstructive Surgery, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. Electronic address:

Mitogen-activated protein kinases (MAPKs) require MAPK phosphatases (MKPs) for deactivation of MAPK intracellular signaling. MKP-1 (encoded by Dusp1) is a key negative regulator of MAPKs and prior reports have indicated that MKP-1 regulates oral cancer-associated inflammation and leukocyte infiltration.

Objective: To determine the significance of myeloid-based expression of MKP-1 in oral cancer.

Methods: The Cancer Genome Atlas (TCGA) was used to address DUSP1 expression in oral squamous cell carcinoma (OSCC). Syngeneic and carcinogen-induced mouse models using global and myeloid-specific Dusp-1 deficient mice with immunophenotypic, histologic, and transcriptomic analyses and in vitro migration assays.

Results: Data from TCGA indicates the DUSP1 expression is inversely related to oral cancer burden and nodal involvement. Using murine models of OSCC, the role of MKP-1 signaling in tumor associated macrophages (TAMs) was assessed. Dusp1-deficient mice had increased tumor burden and TAM infiltrate with increased M2 macrophage polarization. Transcriptomic signatures of TAMs from Dusp1-deficent mice indicated a pro-metastatic phenotype as well as concomitant differences in myeloid-associated genes, cytokine/chemokine signaling, and Notch signaling consistent with tumor progression. In vitro and in vivo assays revealed mouse OSCC cells had a higher migration rate using TAM cell-free supernatant from Dusp1 deficiency mice compared to controls with enhanced regional cervical lymph node metastasis, respectively. To validate TAM studies using implantable mouse models, an OSCC progression model with conditional myeloid-specific Dusp-1 deficient mice demonstrated enhanced OSCC disease progression, characterized by advanced onset, histological stage, and tumor burden.

Conclusion: Myeloid-based Dusp1-deficiency increases OSCC burden and metastasis through alteration in TAM recruitment, gene profile, and polarity suggesting that MKP-1 could be a viable target to reprogram TAM to limit local/regional OSCC extension.
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http://dx.doi.org/10.1016/j.oraloncology.2021.105401DOI Listing
June 2021

Digestive properties and effects of Chimonanthus nitens Oliv polysaccharides on antioxidant effects in vitro and in immunocompromised mice.

Int J Biol Macromol 2021 Aug 22;185:306-316. Epub 2021 Jun 22.

Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

The study was aimed to investigate the simulated digestion behavior of the bioactive polysaccharides from Chimonanthus nitens Oliv (COP1), antioxidant activity in vitro, and prevention against cyclophosphamide (CP) induced oxidative damage in mice. The results showed that COP1 were 18.843 kDa, and consisted of arabinose (56.6 mol%), galactose (24.9 mol%), xylose (11.1 mol%), and glucose (7.4 mol%). Gastrointestinal digestion significantly improved the radical (DPPH, OH, and ABTS) scavenging activities of COP1. Meanwhile, administration of COP1 (150, 300, and 600 mg/kg, continuous 16 days) prevented hepatotoxicity in CP-induced mice (reducing liver index and transaminase levels, alleviating liver damage). COP1 also attenuated oxidative stress as evident from as shown by reduced levels of MDA and enhanced activity of antioxidant enzymes (CAT, SOD, and GSH-Px). In addition, COP1 regulated the Nrf2/Keap1 signaling pathway in CP-treated mice, decreasing the upstream factor Keap1 and increasing the upstream factor Nrf2, which in turn enhanced the expression of downstream factors (NQO1, HO-1, GSH-Px, SOD1, and CAT). COP1 also protected the body from CP-induced oxidative damage by down-regulating Bax and caspase3 in the apoptosis pathway and up-regulating Bcl-2 mRNA levels. Overall, COP1 might be harnessed as an effective natural antioxidant for medical and food industries.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.114DOI Listing
August 2021

Melanoma With an Unknown Primary in an Asymptomatic Elderly Male With Unilateral Lymphadenopathy.

Cureus 2021 May 20;13(5):e15140. Epub 2021 May 20.

Department of Radiology, University of Texas Medical Branch, Galveston, USA.

Melanoma with an unknown primary (MUP) is an uncommon metastatic melanoma without an obvious primary site. MUP has a higher prevalence in men in their fifth decade of life. The pathogenesis of MUP is still unknown but several hypotheses have been proposed including the predominant regression theory, occult cutaneous, or visceral location, or by the presence of ectopic melanocytes. Proper physical examination, imaging, and histopathological review are needed to diagnose MUP. Patients with MUP must be aggressively treated and monitored for recurrence. We present a case of MUP occurring in an asymptomatic 61-year-old male with axillary lymphadenopathy. We hope to raise awareness that melanoma of unknown primary can present in lymph nodes without external structural changes.
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http://dx.doi.org/10.7759/cureus.15140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212892PMC
May 2021

OGT regulated O-GlcNAcylation promotes papillary thyroid cancer malignancy via activating YAP.

Oncogene 2021 Jul 21;40(30):4859-4871. Epub 2021 Jun 21.

Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, 200040, China.

The incidence of thyroid cancer is growing rapidly during the past decades worldwide. Although most thyroid tumors are curable, some patients diagnosed with distant metastases are associated with poor prognosis. The molecular mechanisms underlying these cases are still largely unknown. Here we found that the upregulated O-Linked N-Acetylglucosamine Transferase (OGT) expression and O-GlcNAcylation (O-GlcNAc) modification in papillary thyroid cancer (PTC) were essential in tumor growth and metastasis. Mass spectrometry analysis showed that YAP was the effector protein modified by OGT. In details, YAP Ser109 O-GlcNAcylation promoted the malignant phenotypes in PTC cells by inducing YAP Ser127 dephosphorylation and activation. Our work clearly showed the critical role of OGT and YAP played in PTC tumors and made it possible for us to seek the clinical potential of manipulating OGT/YAP activity in PTC targeted therapies. These findings also confirmed OGT worked in collaboration with classical Hippo pathway kinases as an upstream regulator of YAP in PTC tumors.
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http://dx.doi.org/10.1038/s41388-021-01901-7DOI Listing
July 2021

Probabilistic shaping based constellation encryption for physical layer security in OFDM RoF system.

Opt Express 2021 Jun;29(12):17890-17901

The physical layer security of radio-over-fiber (RoF) system is a very important problem for future communication. In this paper, a novel probabilistic shaping (PS) based constellation encryption scheme is proposed in which two bit-level encryption operations are firstly performed according to chaotic sequences and hash values. The chaotic sequences are generated by hyperchaotic system and hash values are obtained by SHA-512. Then PS is applied to enhance transmission performance. After PS-16-quadrature amplitude modulation (QAM), constellation encryption is implemented aiming at maintaining overall shaping distribution unchanged and improving security. An encrypted PS-16-QAM orthogonal frequency division multiplexing (OFDM) signal is successfully transmitted over 50 km standard single-mode fiber (SSMF) and 5 m wireless channel in our experiment. The results demonstrate that the key space of 10 is achieved to defend malicious attacks. Moreover, the proposed PS-based encryption scheme can obtain approximately 2.4 dB gain at a BER of 10 compared with traditional OFDM signal. Thus, the proposed scheme has a good application prospect in the future OFDM-RoF system due to the dominant BER and security performance.
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http://dx.doi.org/10.1364/OE.424661DOI Listing
June 2021

Study of the association of interferon-γ gene polymorphisms and Th1/Th2 balance in tuberculosis susceptibility.

Am J Transl Res 2021 15;13(5):5533-5539. Epub 2021 May 15.

Department of Tuberculosis, The Second Affiliated Hospital of Hainan Medical College Haikou 570311, Hainan, China.

Objectives: This study investigated and analyzed the association of interferon-γ (IFN-γ) gene polymorphisms and Th1/Th2 balance in tuberculosis susceptibility.

Methods: From January 2018 to January 2020, 121 tuberculosis patients that were hospitalized for initial treatment and were chosen as the pulmonary tuberculosis group, and another 80 healthy individuals with physical examination in the same period were selected as the control group. The genetic distribution of IFN-γ +874 sites in the two sets of subjects was detected by sequence-specific primer PCR (PCR-SSP), the serum IFN-γ level was detected by ELISA, and the ratio of Th1 and Th2 on cells in two sets of subjects were detected by flow cytometry. Subsequently, we compared the differences in IFN-γ degree and the changes in Th1/Th2 in patients with different genotypes.

Results: The difference in the distribution of IFN-γ (+874 T/A) genotype frequencies between the two groups of subjects was significantly different (), and the frequency of A allele in the tuberculosis group was clearly higher than that in the control group (). Compared with the control group, the Th1 cells percentage in the pulmonary tuberculosis group decreased remarkably, the Th2 cells percentage increased significantly, while the Th1/Th2 ratio was notably decreased (). In addition, the Th1/Th2 ratio in tuberculosis patients with an IFN-γ (+874 T/A) genotype AA was remarkably lower than those with AT+TT genotype ().

Conclusion: An AA genotype with IFN-γ (+874 T/A) is a susceptible genotype for tuberculosis. The mechanism may be related to the imbalance of Th1/Th2 which is more likely to occur in patients with AA genotype. This can lead to a decline in immune function of the body, making it more susceptible to infection with mycobacterium which induces tuberculosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205772PMC
May 2021

Water Microdroplets Allow Spontaneously Abiotic Production of Peptides.

J Phys Chem Lett 2021 Jun 16;12(24):5774-5780. Epub 2021 Jun 16.

School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China.

The chemistry of abiotic synthesis of peptides in the context of their prebiotic origins is a continuing challenge that arises from thermodynamic and kinetic constraints in aqueous media. Here we reported a strategy of microdroplets' mass spectrometry for peptide bonds formed from pure amino acids or a mixture in the presence of phosphoric acids in aqueous microdroplets. In contrast to bulk experiments, the condensation reactions proceed spontaneously under ambient conditions. The microdroplet gave a negative free-energy change (Δ ∼ -1.1 kcal/mol), and product yields of ∼75% were obtained at the scale of a few milliseconds. Experiments in which nebulization gas pressure and external charge were varied established dependence of peptide production on the droplet size that has a high surface-to-volume ratio. It is concluded that the condensation reactions occurred at or near the air-water interfaces of microdroplets. This aqueous microdroplets approach also provides a route for chemistry synthesis in the prebiotic era.
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http://dx.doi.org/10.1021/acs.jpclett.1c01083DOI Listing
June 2021
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