Publications by authors named "Jing Feng"

917 Publications

The relationship between prescription of ultrafiltration and intradialytic hypotension in Chinese hemodialysis patients.

Ann Palliat Med 2021 May 12. Epub 2021 May 12.

Department of Traditional Chinese Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: Intradialytic hypotension (IDH) remains the most frequent severe side effect of hemodialysis (HD) and increases patient morbidity and mortality. Excessive ultrafiltration (UF) is considered the leading cause of IDH. This study developed a suitable prescription of UF to reduce the incidences of IDH episodes.

Methods: A retrospective study was performed to analyze 33,224 HD/hemodiafiltration (HDF) treatments in 312 patients. The prescription of UF were determined following the International Society of Peritoneal Dialysis (ISPD) guideline. The Pearson's method was used to study the correlation between relative variables. The receiver operating characteristic (ROC) curve was used to predict the value of the UF/weight ratio (UF/Wt) for IDH in all patients to establish a diagnostic cut-off point. Univariate and multivariate logistic regression analyses were applied to study the risk factors of IDH.

Results: Twelve thousand five hundred and fifty-eight sessions of IDH (38.7%) were identified, among which 1,224 (3.6%) were recorded with intervention against IDH. Both the systolic blood pressure (SBP) and mean arterial pressure (MAP) of the hemodialytic patients were positively correlated with the UF quantity and the UF/Wt, but negatively correlated with blood flow. The ROC curve showed that UF/Wt =0.04 was the cut-off point for IDH. Age [per 10-year increment, odds ratio (OR) =1.005, 95% confidence interval (CI): 1.004 to 1.007, P=0.000], diabetes mellitus (OR =1.209, 95% CI: 1.122 to 1.303, P=0.000), and UF/Wt >0.04 (OR =1.605, 95% CI: 1.532 to 1.682, P=0.000) were all independently associated with higher incidences of IDH.

Conclusions: IDH commonly occurs during HD in Chinese patients. Unchangeable factors such as diabetes and age, and modifiable factors including UF were associated with IDH. A UF/Wt threshold more than 0.04 may be a potential alert for avoiding IDH, especially in the elderly and diabetic patients.
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http://dx.doi.org/10.21037/apm-21-791DOI Listing
May 2021

Reduced mother-to-child transmission of hepatitis B after implementation of completely charge-free active-passive immunoprophylaxis: an observational cohort study.

Expert Rev Vaccines 2021 May 7. Epub 2021 May 7.

Departments of Laboratory Medicine and Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing University Medical School, Jiangsu province, China.

Objectives: China has implemented universal hepatitis B vaccination since 2002 and provided charge-free hepatitis B immunoglobulin (HBIG) to infants of HBV-infected mothers since July 2011. We aimed to compare mother-to-child transmission (MTCT) in children born before and since July 2011.

Methods: In total, 5,149 children of HBV-infected mothers were tested for HBV markers. Group one contained 1,160 children born during August 2002-June 2011 and group two contained 3,989 children born during July 2011-June 2016.

Results: In total, 92 (1.8%, 95% confidence interval [95%CI] 1.4-2.2) children were infected with HBV. None (0%, 95%CI 0.0-0.1) of 3,716 children of mothers with negative hepatitis B e antigen (HBeAg) was infected, whereas 92 (6.4%, 95%CI 5.2-7.8) of 1,433 children of HBeAg-positive mothers were infected (p<0.0001). Among children of HBeAg-positive mothers, MTCT occurred in 10.3% (19/185) (95%CI 6.3-15.6) in group one and 5.8% (73/1,248) (95%CI 4.6-7.3) in group two (p=0.02).

Conclusions: Implementing charge-free active-passive immunoprophylaxis greatly reduces MTCT of HBV in children of HBeAg-positive mothers, highlighting the importance of timely administration of both hepatitis B vaccine and HBIG to prevent MTCT. The still remaining MTCT suggests that reducing maternal virus load before delivery is an additional important measure.
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http://dx.doi.org/10.1080/14760584.2021.1927723DOI Listing
May 2021

Application of metagenomic next-generation sequencing (mNGS) combined with rapid on-site cytological evaluation (ROSCE) for the diagnosis of Chlamydia psittaci pneumonia.

Int J Clin Exp Pathol 2021 15;14(4):389-398. Epub 2021 Apr 15.

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital Tianjin 300052, China.

We evaluated the application of metagenomic next-generation sequencing (mNGS) combined with rapid on-site cytological evaluation (ROSCE) in the diagnosis of pneumonia, to provide a basis for an accurate diagnosis. Clinical data from three patients with pneumonia diagnosed by the combination of mNGS and ROSCE from June 2019 to June 2020 in the Department of Respiratory and Critical Care Medicine of Tianjin Medical University General Hospital were reviewed. Three patients with community-acquired pneumonia failed to respond to the initial treatment, and were finally diagnosed by bronchoscopic lung biopsy and alveolar lavage fluid (BALF) mNGS. According to the ROSCE cytologic characteristics, the scope of the lesions was narrowed to inflammatory lesions excluding tumor and other non-infectious lesions. Subsequently, mNGS results confirmed that the three patients were infected by C. psittaci, sequence numbers 2066, 126, and 1077, respectively. Two patients developed severe pneumonia and required organ function support. The other patient had recurrent high fever and severe headache, which significantly complicated clinical diagnosis and treatment. Eventually, the treatment plan was adjusted according to the mNGS results, resulting in gradual improvement of symptoms and satisfactory prognosis. mNGS combined with ROSCE is effective for the accurate diagnosis and treatment of pneumonia, having significant advantages in comparison with other detection methods, particularly in the cases of rare pathogens, mixed pathogen infections, and immunodeficient patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085828PMC
April 2021

Proteomics and transcriptomics jointly identify the key role of oxidative phosphorylation in fluoride-induced myocardial mitochondrial dysfunction in rats.

Ecotoxicol Environ Saf 2021 Apr 28;218:112271. Epub 2021 Apr 28.

School of Public Health, Shanxi Medical University, Taiyuan 030001, Shanxi, China. Electronic address:

The regulation of mitochondrial function, which is dominated by oxidative phosphorylation (OXPHOs), is important in fluoride induced cardiovascular disease. Based on the previous study of fluoride-induced mitochondrial structure and membrane potential abnormalities, this study integrated ITRAQ protein quantification and RNA-Seq methods to analyze the sequencing data of rat myocardial tissue under fluoride exposure (0, 30, 60 and 90 mg/L). A total of 22 differentially expressed genes associated with the OXPHOs pathway were screened by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) co-enrichment analysis, and were localizated by Interaction Network and calculated inter-genes and inter-omics correlations by Pearson correlation. In general, fluoride exposure can down-regulate genes related OXPHOs, particularly affecting the assembly of the complex I including Ndufa10, resulting in abnormal mitochondrial ATP synthesis and reduced myocardial energy supply. Most importantly, this study shows that the enriched information from the proteomics can explain the change process of energy production, but the specific molecules involved in energy supply cannot be obtained via transcriptomics information alone. Based on the results of transcriptional and protein analysis, our findings contribute to an innovative understanding of the pathways and molecular changes of myocardial injury induced by fluorosis.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112271DOI Listing
April 2021

Luminescence Change from Orange to Blue for Zero-Dimensional Cs InCl (H O) Metal Halides in Water and a New Post-doping Method.

Chem Asian J 2021 May 1. Epub 2021 May 1.

Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, School of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, P. R. China.

Zero-dimensional metal halides have attracted much attention due to their attractive photoelectric properties. Here, we propose a new strategy of synthesizing metal halides crystals by recrystallization in water. The as-synthesized Cs InCl (H O)-orange crystals are dissolved and recrystallized in water (Cs InCl (H O)-blue), with its photoluminescence (PL) changing from orange to blue, both of which are derived from self-trapping excitons (STEs). The time-resolved photoluminescence (TRPL) spectrum of Cs InCl (H O)-blue shows that it has an ultralong lifetime up to milliseconds (τ=52.98 ms), which is expected to be applied in biological sensors. The photoluminescence quantum yield (PLQY) increases from 2.25% to 11.61% in the self-assembly process. By using a post-doping method, the PL of crystals turns into red when we introduce Mn as dopant while there is no obvious change upon using a traditional solvent-thermal method. Recrystallization in water and post-doping provide a new perspective for the synthesis and doping of metal halides.
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http://dx.doi.org/10.1002/asia.202100293DOI Listing
May 2021

Numerical Optimization for the Geometric Configuration of Ceramics Perform in HCCI/ZTA Wear-Resistant Composites Based on Actual Particle Model.

Nanoscale Res Lett 2021 Apr 29;16(1):71. Epub 2021 Apr 29.

Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming, 650093, China.

In order to reduce the thermal stress in high chromium cast iron (HCCI) matrix composites reinforced by zirconia toughened alumina (ZTA) ceramic particles, finite element simulation is performed to optimize the geometric configuration of ceramics perform. The previous model simplifies the overall structure of the ceramic particle preform and adds boundary conditions to simulate the particles, which will cause uncontrollable error in the results. In this work, the equivalent grain models are used to describe the actual preform, making the simulation results closer to the actual experimental results. The solidification process of composite material is simulated, and the infiltration between molten iron and ceramic particles is realized. Thermal stress in solidification process and compression stress distribution are obtained. The results show that adding 10-mm round holes on the preform can improve the performance of the composite, which is helpful to prevent the cracks and increases the plasticity of the material.
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http://dx.doi.org/10.1186/s11671-021-03514-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085194PMC
April 2021

Association of professional identity, job satisfaction and burnout with turnover intention among general practitioners in China: evidence from a national survey.

BMC Health Serv Res 2021 Apr 26;21(1):382. Epub 2021 Apr 26.

Department of Social Medicine and Health Management, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan, 430030, Hubei, China.

Background: The complex interrelationships between professional identity, job satisfaction, burnout, and turnover intention among general practitioners (GPs) are insufficiently understood in China. This study aimed to investigate the interrelationships between professional identity, job satisfaction, burnout, and turnover intention in China, and to examine whether job satisfaction and burnout played mediating roles between professional identity and turnover intention.

Methods: A cross-sectional survey was conducted between October, 2017 and February, 2018 in China. The participants were selected using a multistage stratified random sampling method. Data were collected with a self-administered questionnaire from 3236 GPs (response rate, 99.8%) working in community health institutions in China. Professional identity was measured by the 13 items scale, and job satisfaction scale with an 11-item designed by Shi et al. was employed. Burnout was measured using a 22-item Maslach Burnout Inventory-Human Services Survey, and turnover intention was measured with a 6 items scale. Descriptive statistics were calculated and groups' differences were estimated Student's t-test and analyses of variance. Pearson's correlation analysis was used to assess the degree of correlation among different dimensions of professional identity, job satisfaction, burnout, and turnover intention. Structural equation modeling analysis was applied to examine the interrelationships among these study variables based on the hypothesized model.

Results: The proposed model achieved a good model fit. Job satisfaction had a direct negative effect on turnover intention (β = - 0.38, P < 0.001), burnout had a direct positive effect on turnover intention (β = 0.37, P < 0.001), and professional identity had an indirect negative effect on turnover intention through the mediating effect of job satisfaction and burnout.

Conclusions: Our study elucidated the pathways linking professional identity, job satisfaction, and burnout to turnover intention of GPs. This revealed that turnover intention was significantly affected by job satisfaction and burnout, and the effects of professional identity on turnover intention can be mediated by job satisfaction and burnout.
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http://dx.doi.org/10.1186/s12913-021-06322-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074426PMC
April 2021

Mechanisms of broad-band ultraviolet B irradiation-induced itch in mice.

J Invest Dermatol 2021 Apr 1. Epub 2021 Apr 1.

Department of Anesthesiology, The Center for the Study of Itch & Sensory Disorders, Washington University School of Medicine, St. Louis, MO, USA.

Although sunburn can produce severe uncontrollable itching, the underlying mechanisms of ultraviolet (UV) irradiation-induced itch are poorly understood because of a lack of experimental animal models of sunburn itch. Here we established a sunburn-related mouse model and found that Broad-band UVB (BB-UVB) irradiation elicited scratching but not wiping behavior in mice. By using a combination of live-cell Ca imaging and quantitative RT-PCR on dorsal root ganglion (DRG) neurons, hematoxylin and eosin staining, immunofluorescence staining of skin preparations, behavioral testing, in combination with genetic and pharmacological approaches, we showed that TRPV1-positive DRG neurons but not mast cells are involved in BB-UVB irradiation-induced itch. Moreover, both genetic and pharmacological inhibition of TRPV1 function significantly alleviated BB-UVB irradiation-induced itch response. Collectively, our results suggest that BB-UVB irradiation evokes itch sensation in mice through promoting TRPV1 channel function in DRG neurons and provide potential therapeutic targets for sunburn-related itch.
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http://dx.doi.org/10.1016/j.jid.2021.03.015DOI Listing
April 2021

Local administration of zoledronic acid prevents traumatic osteonecrosis of the femoral head in rat model.

J Orthop Translat 2021 Mar 8;27:132-138. Epub 2021 Mar 8.

Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Laboratory (No BZ0128), Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, Beijing, 100853, China.

Background: Osteonecrosis of the femoral head (ONFH) is a refractory disease due to its unclear pathomechanism. Neither conservative treatment nor surgical treatment during the early stage of ONFH achieves satisfactory results. Therefore, this study aims to explore the available evidence on the effect of zoledronic acid on early-stage ONFH.

Methods: For groups were established:the Normal group, model group, Normal saline group(NS group) and zoledronic acid-treated group The blood supply to the femoral head of animals in the model group and zoledronic acid-treated group was interrupted via a surgical procedure, and zoledronic acid was then locally administered to the femoral head. Four weeks after surgery, all the hips were harvested and evaluated by micro-CT and histopathology(H&E staining, TRAP staining, Toluidine blue staining and masson staining).

Results: The values of BMD, BS/BV and Tb.Th in the Normal group and zoledronic acid-treated group were significantly higher than those in the model group and NS group (p ​< ​0.05). The outcome of H&E staining, Toluidine blue staining and masson staining were consistent with that of micro-CT.

Conclusion: The local administration of zoledronic acid in the femoral head had positive effects on the bone structure of the femoral head in a modified rat model of traumatic ONFH and offered a promising therapeutic strategy during the early stage of ONFH.

The Translational Potential Of This Article: This article could provide a choice for treating patients who have osteonecrosis of femora head and can be the basic research for advanced development over this disease.
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http://dx.doi.org/10.1016/j.jot.2020.08.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972932PMC
March 2021

Responsive optical probes for deep-tissue imaging: Photoacoustics and second near-infrared fluorescence.

Adv Drug Deliv Rev 2021 Mar 25;173:141-163. Epub 2021 Mar 25.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Optical imaging has played a vital role in development of biomedicine and image-guided theragnostic. Nevertheless, the clinical translation of optical molecular imaging for deep-tissue visualization is still limited by poor signal-to-background ratio and low penetration depth owing to light scattering and tissue autofluorescence. Hence, to facilitate precise diagnosis and accurate surgery excision in clinical practices, the responsive optical probes (ROPs) are broadly designed for specific reaction with biological analytes or disease biomarkers via chemical/physical interactions for photoacoustic and second near-infrared fluorescence (NIR-II, 900-1700 nm) fluorescence imaging. Herein, the recent advances in the development of ROPs including molecular design principles, activated mechanisms and treatment responses for photoacoustic and NIR-II fluorescence imaging are reviewed. Furthermore, the present challenges and future perspectives of ROPs for deep-tissue imaging are also discussed.
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http://dx.doi.org/10.1016/j.addr.2021.03.008DOI Listing
March 2021

CD146 is a Novel ANGPTL2 Receptor that Promotes Obesity by Manipulating Lipid Metabolism and Energy Expenditure.

Adv Sci (Weinh) 2021 Mar 27;8(6):2004032. Epub 2021 Jan 27.

Key Laboratory of RNA Biology Key Laboratory of Protein and Peptide Pharmaceuticals Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China.

Obesity and its related complications pose an increasing threat to human health; however, targetable obesity-related membrane receptors are not yet elucidated. Here, the membrane receptor CD146 is demonstrated to play an essential role in obesity. In particular, CD146 acts as a new adipose receptor for angiopoietin-like protein 2 (ANGPTL2), which is thought to act on endothelial cells to activate adipose inflammation. ANGPTL2 binds to CD146 to activate cAMP response element-binding protein (CREB), which then upregulates CD146 during adipogenesis and adipose inflammation. CD146 is present in preadipocytes and mature adipocytes, where it is mediated by its ligands ANGPTL2 and galectin-1. In preadipocytes, CD146 ablation suppresses adipogenesis, whereas the loss of CD146 in mature adipocytes suppresses lipid accumulation and enhances energy expenditure. Moreover, anti-CD146 antibodies inhibit obesity by disrupting the interactions between CD146 and its ligands. Together, these findings demonstrate that ANGPTL2 directly affects adipocytes via CD146 to promote obesity, suggesting that CD146 can be a potential target for treating obesity.
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http://dx.doi.org/10.1002/advs.202004032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967059PMC
March 2021

Momentum space toroidal moment in a photonic metamaterial.

Nat Commun 2021 Mar 19;12(1):1784. Epub 2021 Mar 19.

School of Physics and Astronomy, University of Birmingham, Birmingham, UK.

Berry curvature, the counterpart of the magnetic field in the momentum space, plays a vital role in the transport of electrons in condensed matter physics. It also lays the foundation for the emerging field of topological physics. In the three-dimensional systems, much attention has been paid to Weyl points, which serve as sources and drains of Berry curvature. Here, we demonstrate a toroidal moment of Berry curvature with flux approaching to π in judiciously engineered metamaterials. The Berry curvature exhibits a vortex-like configuration without any source and drain in the momentum space. Experimentally, the presence of Berry curvature toroid is confirmed by the observation of conical-frustum shaped domain-wall states at the interfaces formed by two metamaterials with opposite toroidal moments.
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http://dx.doi.org/10.1038/s41467-021-22063-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979886PMC
March 2021

Supercontinuum generation of 314.7  W ranging from 390 to 2400  nm by tapered photonic crystal fiber.

Opt Lett 2021 Mar;46(6):1429-1432

To consider both high-power handling and blue-extended supercontinuum (SC) generation, a long-tapered photonic crystal fiber is pumped by a high-power laser source. An SC ranging from 390 to 2400 nm with 314.7 W output power is obtained. A spectral component below 960 nm accounts for 36.1% of the total output power, exceeding 113.5 W, with a spectral flatness within 16 dB. To the best of our knowledge, this is the first time an SC coverage of all visible wavelengths with more than 300 W output power has been achieved. This result increases the output power of the SC covering the visible range by a factor of three.
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http://dx.doi.org/10.1364/OL.420707DOI Listing
March 2021

Modulation of corticosterone and changes of signal molecules in the HPA axis after cold water swimming stress.

Anim Cells Syst (Seoul) 2021 Feb 23;25(1):37-45. Epub 2021 Feb 23.

Department of Pharmacology and Institute of Natural Medicine, College of Medicine, Hallym University, Chun-Cheon, South Korea.

In the present study, we examined the effect of cold-water swimming stress (CWSS) on plasma corticosterone levels. Mice were exposed to stress in 4°C for 3 mins. Plasma corticosterone (CORT) level was measured at 0, 15, and 30 min after stress stimulation. The plasma CORT level was gradually increased up to 30 min. Then we further examined the changes of several signaling molecules expression levels, such as p-ERK, p-JNK, p-P38, p-AMPKα1, p-AMPKα2, and p-mTOR, in the HPA axis. We observed that those signaling molecules were altered after stress in the HPA axis. p-ERK, p-JNK, p-P38, and p-mTOR proteins expression were reduced by CWSS in the HPA axis. However, the phosphorylation of AMPKα1 and AMPKα2 were activated after CWSS in the HPA axis. Our results suggest that the upregulation of plasma CORT level induced by CWSS may be modulated by the those signaling molecules.
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http://dx.doi.org/10.1080/19768354.2021.1890211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935129PMC
February 2021

gene as a novel pathogenic mutation occurring during the transformation of colorectal adenoma into colorectal cancer.

J Gastrointest Oncol 2021 Feb;12(1):69-78

Department of Oncology, The Second Hospital of Shanxi Medical University, Taiyuan, China.

Background: Polyps may develop into colorectal cancer (CRC) after 10-20 years. The occurrence of polyps and tumors caused by somatic gene mutations is considered a main pathogenesis of CRC. Among all general patients with polyps or CRC, some had adenoma of varying degrees that were consistent with familial colorectal adenomas. A patient with CRC (the propositus) and his brothers and sister, all of whom had varying degrees of colorectal polyps showed different adenomas with different members in a family.

Methods: In the present study, a total of 9 family members were investigated, and a family tree was drawn. Genomic DNA was extracted from peripheral venous blood samples from family members, and whole-exome sequencing (WES) and Sanger sequencing were performed on the DNA samples. The result of WES was compared with compared directly to the reference genome (hg19) with Burrows-Wheeler Aligner, which is as control group from.

Results: We identified a base substitution in the gene (c.68415368T>G, chromosome 9 q13), predicted the target gene of miR-4477b through the biologic website, and analyzed the Gene Ontology (GO) and signal pathway of the target gene. The GO functional annotation analysis of the target gene of mir4477b revealed that these genes are involved mainly in the G1/S transition of the mitotic cell cycle, activation of mitogen-activated protein kinase activity, protein phosphorylation, and membrane, centrosome, cytoplasm, zinc ion-binding, protein-binding, and ligase activity. Kyoto Encyclopedia of Gene and Genomes pathway analysis revealed that miR-4477b regulates target genes mainly involved in the phosphoinositide 3-kinase/Akt signaling pathway, regulation of the actin cytoskeleton, proteoglycans in cancer, pathways in cancer, and renal cell carcinoma.

Conclusions: The mutation of the gene likely leads to the occurrence of adenoma and CRC. In-depth studies of patients from the same family with different stages of adenoma can avoid errors caused by gene diversity, incomplete clinical data, and uncertain disease development. The gene may represent a key gene mutation in colorectal carcinogenesis and a multiyear cancer risk for patients that requires further attention.
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http://dx.doi.org/10.21037/jgo-20-600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944151PMC
February 2021

Intermittent hypoxia: Friend or foe on endothelial repair in mouse model.

Exp Lung Res 2021 May-Jun;47(5):211-225. Epub 2021 Mar 6.

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin, China.

Obstructive sleep apnea, which is characterized by intermittent hypoxia (IH), is a common respiratory disease. The aim of the present study was to explore the relationship between hypoxia and endothelial progenitor cell (EPC) function, and explain the role of IH in endothelial repair. Peripheral blood mononuclear cells (PBMCs) were isolated from a mouse model of IH. The number of CD133 kinase insert domain receptor (KDR), CD133CD34, CD34KDR and ALDHCD34KDR EPCs was determined by flow cytometry. HIF-1α, stromal-derived factor-1 (SDF-1) α and VEGF were measured by ELISA. The proliferative ability of PBMCs was determined. EPC migration was assessed by Transwell assay and surface proteins by western blot analysis. EPCs were co-cultured with mouse brain endothelial cells and their angiogenic ability was analyzed. The number of CD133KDR, CD133CD34 and CD34KDR EPCs increased with IH ingravescence. The number of ALDHCD34KDR EPCs with mild IH stimulation was higher and gradually decreased in the moderate and severe IH groups. The release of HIF-1α, SDF-1α and VEGF in the serum increased with the increase in the degree of IH. In the mild IH treatment, the migration and angiogenesis of EPCs, as well as the expression of vascular endothelial growth factor receptor 2 and cysteine-X-cysteine receptor 4, were higher than those in the control group, but progressively decreased in the groups with moderate and severe IH.: Increased levels of IH accelerated the increase in vasoactive factors in peripheral blood, thereby mobilizing a large number of EPCs. Increasing of IH diminished the mobilization, chemotactic and angiogenetic ability of EPCs.
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http://dx.doi.org/10.1080/01902148.2021.1891355DOI Listing
March 2021

Combination of umbilical cord mesenchymal stem cells and standard immunosuppressive regimen for pediatric patients with severe aplastic anemia.

BMC Pediatr 2021 Feb 27;21(1):102. Epub 2021 Feb 27.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Heping District, Tianjin, 300020, China.

Background: Defects of bone marrow mesenchymal stem cells (BM-MSCs) in proliferation and differentiation are involved in the pathophysiology of aplastic anemia (AA). Infusion of umbilical cord mesenchymal stem cells (UC-MSCs) may improve the efficacy of immunosuppressive therapy (IST) in childhood severe aplastic anemia (SAA).

Methods: We conducted an investigator-initiated, open-label, and prospective phase IV trial to evaluate the safety and efficacy of combination of allogenic UC-MSCs and standard IST for pediatric patients with newly diagnosed SAA. In mesenchymal stem cells (MSC) group, UC-MSCs were injected intravenously at a dose of 1 × 10/kg per week starting on the 14th day after administration of rabbit antithymocyte globulin (ATG), for a total of 3 weeks. The clinical outcomes and adverse events of patients with UC-MSCs infusion were assessed when compared with a concurrent control group in which patients received standard IST alone.

Results: Nine patients with a median age of 4 years were enrolled as the group with MSC, while the data of another 9 childhood SAA were analysed as the controls. Four (44%) patients in MSC group developed anaphylactic reactions which were associated with rabbit ATG. When compared with the controls, neither the improvement of blood cell counts, nor the change of T-lymphocytes after IST reached statistical significance in MSC group (both p > 0.05) and there were one (11%) patient in MSC group and two (22%) patients in the controls achieved partial response (PR) at 90 days after IST. After a median follow-up of 48 months, there was no clone evolution occurring in both groups. The 4-year estimated overall survival (OS) rate in two groups were both 88.9% ± 10.5%, while the 4-year estimated failure-free survival (FFS) rate in MSC group was lower than that in the controls (38.1% ± 17.2% vs. 66.7% ± 15.7%, p = 0.153).

Conclusions: Concomitant use of IST and UC-MSCs in SAA children is safe but may not necessarily improve the early response rate and long-term outcomes. This clinical trial was registered at ClinicalTrials.gov, identifier: NCT02218437 (registered October 2013).
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http://dx.doi.org/10.1186/s12887-021-02562-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912947PMC
February 2021

Co-exposure to inorganic arsenic and fluoride prominently disrupts gut microbiota equilibrium and induces adverse cardiovascular effects in offspring rats.

Sci Total Environ 2021 May 29;767:144924. Epub 2021 Jan 29.

Department of Epidemiology and Environmental Health, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY, USA; Department of Pharmacology and Toxicology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA.

Co-exposure to inorganic arsenic (iAs) and fluoride (F) and their collective actions on cardiovascular systems have been recognized as a global public health concern. Emerging studies suggest an association between the perturbation of gut bacterial microbiota and adverse cardiovascular effects (CVEs), both of which are the consequence of iAs and F exposure in human and experimental animals. The aim of this study was to fill the gap of understanding the relationship among co-exposure to iAs and F, gut microbiota perturbation, and adverse CVEs. We systematically assessed cardiac morphology and functions (blood pressure, echocardiogram, and electrocardiogram), and generated gut microbiota profiles using 16S rRNA gene sequencing on rats exposed to iAs (50 mg/L NaAsO), F (100 mg/L NaF) or combined iAs and F (50 mg/L NaAsO + 100 mg/L NaF), in utero and during early postnatal periods (postnatal day 90). Correlation analysis was then performed to examine relationship between significantly altered microbiota and cardiac performance indices. Our results showed that co-exposure to iAs and F resulted in more prominent effects in CVEs and perturbation of gut microbiota profiles, compared to iAs or F treatment alone. Furthermore, nine bacterial genera (Adlercreutzia, Clostridium sensu stricto 1, Coprococcus 3, Romboutsia, [Bacteroides] Pectinophilus group, Lachnospiraceae NC2004 group, Desulfovibrio, and two unidentified genera in Muribaculaceae and Ruminococcaceae family), which differed significantly in relative abundance between control and iAs and F co-exposure group, were strongly correlated with the higher risk of CVEs (correlation coefficient = 0.70-0.88, p < 0.05). Collectively, these results suggest that co-exposure to iAs and F poses a higher risk of CVEs, and the part of the mode of action is potentially through inducing gut microbiota disruption, and the strong correlations between them indicate a high potential for the development of novel microbiome-based biomarkers of iAs and/or F associated CVEs.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144924DOI Listing
May 2021

Cryo-EM structure of a proton-activated chloride channel TMEM206.

Sci Adv 2021 Feb 24;7(9). Epub 2021 Feb 24.

Department of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

TMEM206 has been recently identified as an evolutionarily conserved chloride channel that underlies ubiquitously expressed, proton-activated, outwardly rectifying anion currents. Here, we report the cryo-electron microscopy structure of pufferfish TMEM206, which forms a trimeric channel, with each subunit comprising two transmembrane segments and a large extracellular domain. An ample vestibule in the extracellular region is accessible laterally from the three side portals. The central pore contains multiple constrictions. A conserved lysine residue near the cytoplasmic end of the inner helix forms the presumed chloride ion selectivity filter. Unprecedentedly, the core structure and assembly closely resemble those of the epithelial sodium channel/degenerin family of sodium channels that are unrelated in amino acid sequence and conduct cations instead of anions. Together with electrophysiology, this work provides insights into ion conduction and gating for a new class of chloride channels that is architecturally distinct from previously characterized chloride channel families.
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http://dx.doi.org/10.1126/sciadv.abe5983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904269PMC
February 2021

Multivariant ligands stabilize anionic solvent-oriented α-CsPbX nanocrystals at room temperature.

Nanoscale 2021 Mar 24;13(9):4899-4910. Epub 2021 Feb 24.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.

Cubic phase CsPbX nanocrystals (NCs) are promising candidates for optoelectronic applications. However, their chemical stability heavily depends on the dynamic ionic surface. In this work, based on the interdependency of the ligands and the reaction solvent, a protocol is developed for high-quality α-CsPbX under ambient conditions. Utilizing this method, the size and full width at half maximum of CsPbX NCs can be simply tuned via changing the cationic ligands or reaction solvent, such as CHCl, CHCl, or toluene. One remarkable result is the synthesis of cubic CsPbI NCs, for which large-scale syntheses have not been reported in the literature except for our method, due to significant phase transition at room temperature. Another result is that we have realized ultrasmall sized CsPbCl NCs with emission at 385 nm for the first time. Furthermore, the elimination of reaction solvent (such as ODE, DMSO, DMF) in our protocol reduces the purification-induced surface ligand loss and the irreversible phase transition to a nonfluorescent phase. Our CsPbX NCs show near-perfect photoluminescence quantum yield (PL QY) and long-term stability in the presence of moisture. Further characterization demonstrates that all the ligands, whether the initial paired X type or the degenerated hybrid L-X type, remain perfectly passivating on the defect sites throughout.
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http://dx.doi.org/10.1039/d0nr08697eDOI Listing
March 2021

Role of Hypoxia Inducible Factor-1α in Alzheimer's Disease.

J Alzheimers Dis 2021 ;80(3):949-961

Chongqing Key Laboratory of Neurodegenerative Diseases, Chongqing, China.

Amyloid-β (Aβ) peptides and hyperphosphorylated tau protein are the most important pathological markers of Alzheimer's disease (AD). Neuroinflammation and oxidative stress are also involved in the development and pathological mechanism of AD. Hypoxia inducible factor-1α (HIF-1α) is a transcriptional factor responsible for cellular and tissue adaption to low oxygen tension. Emerging evidence has revealed HIF-1α as a potential medicinal target for neurodegenerative diseases. On the one hand, HIF-1α increases AβPP processing and Aβ generation by promoting β/γ-secretases and suppressing α-secretases, inactivates microglia and reduces their activity, contributes to microglia death and neuroinflammation, which promotes AD pathogenesis. On the other hand, HIF-1α could resist the toxic effect of Aβ, inhibits tau hyperphosphorylation and promotes microglial activation. In summary, this review focuses on the potential complex roles and the future perspectives of HIF-1α in AD, in order to provide references for seeking new drug targets and treatment methods for AD.
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http://dx.doi.org/10.3233/JAD-201448DOI Listing
January 2021

Cognitive measures during walking with and without lower-limb prosthesis: protocol for a scoping review.

BMJ Open 2021 02 18;11(2):e039975. Epub 2021 Feb 18.

Department of Psychology, North Carolina State University, Raleigh, North Carolina, USA

Introduction: Tuning of lower-limb (LL) robotic prosthesis control is necessary to provide personalised assistance to each human wearer during walking. Prostheses wearers' adaptation processes are subjective and the efficiency largely depends on one's mental processes. Therefore, beyond physical motor performance, prosthesis personalisation should consider the wearer's preference and cognitive performance during walking. As a first step, it is necessary to examine the current measures of cognitive performance when a wearer walks with an LL prosthesis, identify the gaps and methodological considerations, and explore additional measures in a walking setting. In this protocol, we outlined a scoping review that will systematically summarise and evaluate the measures of cognitive performance during walking with and without LL prosthesis.

Methods And Analysis: The review process will be guided and documented by CADIMA, an open-access online data management portal for evidence synthesis. Keyword searches will be conducted in seven databases (Web of Science, MEDLINE, BIOSIS, SciELO Citation Index, ProQuest, CINAHL and PsycINFO) up to 2020 supplemented with grey literature searches. Retrieved records will be screened by at least two independent reviewers on the title-and-abstract level and then the full-text level. Selected studies will be evaluated for reporting bias. Data on sample characteristics, type of cognitive function, characteristics of cognitive measures, task prioritisation, experimental design and walking setting will be extracted.

Ethics And Dissemination: This scoping review will evaluate the measures used in previously published studies thus does not require ethical approval. The results will contribute to the advancement of prosthesis tuning processes by reviewing the application status of cognitive measures during walking with and without prosthesis and laying the foundation for developing needed measures for cognitive assessment during walking. The results will be disseminated through conferences and journals.
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http://dx.doi.org/10.1136/bmjopen-2020-039975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896605PMC
February 2021

Excellent thermoelectric performance achieved in BiTe/BiS@Bi nanocomposites.

Chem Commun (Camb) 2021 Mar;57(20):2555-2558

Faculty of Materials Science and Engineering, Kunming University of Science and Technology, Kunming, 650093, China.

A Bi2Te3/Bi2S3@Bi nanocomposite with a network microstructure was successfully synthesized via a hydrothermal method and spark plasma sintering. This composite was constructed from Bi2Te3 nanoparticles and Bi2S3@Bi nanowires, and its network structure is beneficial for obtaining excellent thermoelectric performance. A ZT peak of 1.2 at 450 K was realized for the nanocomposite sample.
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http://dx.doi.org/10.1039/d1cc00119aDOI Listing
March 2021

Mapping of a QTL with major effect on reducing leaf rust severity at the adult plant growth stage on chromosome 2BL in wheat landrace Hongmazha.

Theor Appl Genet 2021 May 7;134(5):1363-1376. Epub 2021 Feb 7.

Department of Plant Pathology, China Agricultural University, Beijing, 100193, People's Republic of China.

Key Message: A major QTL (QLr.cau-2BL) for APR to leaf rust was detected on 2BL; an SSR marker was developed to closely link with QLr.cau-2BL and validated for effectiveness of MAS. The wheat landrace Hongmazha (HMZ) possesses adult plant resistance (APR) to leaf rust. To detect and validate quantitative trait locus (QTL) for the APR, four wheat populations were assessed for leaf rust severity in a total of eight field and greenhouse experiments. The mapping population Aquileja × HMZ (120 recombinant inbred lines, RILs) was genotyped using 90 K SNP markers. A major QTL (QLr.cau-2BL) was detected between the markers IWB3854 and IWB21922 on chromosome 2BL. IWB3854 and IWB21922 were positioned at approximately 531.14 Mb and 616.48 Mb, respectively, on 2BL of IWGSC RefSeq v1.0 physical map. Based on the sequences between 531.14 and 616.48 Mb on 2BL of IWGSC RefSeq v1.0, 415 simple sequence repeat (SSR) markers were developed. These markers and 28 previously published SSR makers were screened; the resulted polymorphic markers were used to genotype the relatively larger population RL6058 × HMZ (371 RILs). QLr.cau-2BL was mapped within a 1.5 cM interval on 2BL map of RL6058 × HMZ, and a marker (Ta2BL_ssr7) was identified to closely link with QLr.cau-2BL. Effectiveness of selection for QLr.cau-2BL based on Ta2BL_ssr7 was validated using two populations (RL6058 × HMZ F and Jimai22 × HMZ BCF). In addition, polymorphism at Ta2BL_ssr7 was detected among a panel of 282 commercial wheat cultivars. We believe, therefore, that Ta2BL_ssr7 should be useful for introducing QLr.cau-2BL into commercial wheat cultivars and for accumulating QLr.cau-2BL with other APR QTL.
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http://dx.doi.org/10.1007/s00122-021-03776-4DOI Listing
May 2021

Role of various imbalances centered on alveolar epithelial cell/fibroblast apoptosis imbalance in the pathogenesis of idiopathic pulmonary fibrosis.

Chin Med J (Engl) 2021 Jan 14;134(3):261-274. Epub 2021 Jan 14.

Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.

Abstract: There have been recent extensive studies and rapid advancement on the pathogenesis underlying idiopathic pulmonary fibrosis (IPF), and intricate pathogenesis of IPF has been suggested. The purpose of this study was to clarify the logical relationship between these mechanisms. An extensive search was undertaken of the PubMed using the following keywords: "etiology," "pathogenesis," "alveolar epithelial cell (AEC)," "fibroblast," "lymphocyte," "macrophage," "epigenomics," "histone," acetylation," "methylation," "endoplasmic reticulum stress," "mitochondrial dysfunction," "telomerase," "proteases," "plasminogen," "epithelial-mesenchymal transition," "oxidative stress," "inflammation," "apoptosis," and "idiopathic pulmonary fibrosis." This search covered relevant research articles published up to April 30, 2020. Original articles, reviews, and other articles were searched and reviewed for content; 240 highly relevant studies were obtained after screening. IPF is likely the result of complex interactions between environmental, genetic, and epigenetic factors: environmental exposures affect epigenetic marks; epigenetic processes translate environmental exposures into the regulation of chromatin; epigenetic processes shape gene expression profiles; in turn, an individual's genetic background determines epigenetic marks; finally, these genetic and epigenetic factors act in concert to dysregulate gene expression in IPF lung tissue. The pathogenesis of IPF involves various imbalances including endoplasmic reticulum, telomere length homeostasis, mitochondrial dysfunction, oxidant/antioxidant imbalance, Th1/Th2 imbalance, M1-M2 polarization of macrophages, protease/antiprotease imbalance, and plasminogen activation/inhibition imbalance. These affect each other, promote each other, and ultimately promote AEC/fibroblast apoptosis imbalance directly or indirectly. Excessive AEC apoptosis and impaired apoptosis of fibroblasts contribute to fibrosis. IPF is likely the result of complex interactions between environmental, genetic, and epigenetic factors. The pathogenesis of IPF involves various imbalances centered on AEC/fibroblast apoptosis imbalance.
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http://dx.doi.org/10.1097/CM9.0000000000001288DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846426PMC
January 2021

Broilers' head behavior as an early warning index of production and lung health under ammonia exposure.

Poult Sci 2021 Mar 10;100(3):100814. Epub 2020 Nov 10.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, State Key Laboratory of Animal Nutrition, Beijing 100193, China.

This study investigated the effects of ammonia exposure (0, 15, 25, and 35 ppm) on head behavior, production performance and lung tissue morphology of broilers, and the relationship between head behavior, production performance, and lung tissue injury. In this experiment, a total of 264 AA commercial male broilers (21 d old) were assigned to 4 treatment groups with 6 replicates of 11 chickens for a 21-day trial period, the frequency of head-scratching and head-shaking behavior at the initial stage (2, 24, and 72 h) of ammonia exposure were recorded, and the production performance indices and the lung pathological changes after 21 d of ammonia exposure were observed. The correlation analysis was established between head behavior and production performance indices. Results showed that head-scratching behavior increased under 15 ppm ammonia for 72 h, head-shaking behavior increased when exposure to 15 ppm ammonia for 2, 24, and 72 h, and lung tissue was injured when exposure to 15 ppm ammonia for 21 d. However, exposure to 15 ppm ammonia did not influence growth performance. Compared with the control group, exposure to 25 ppm decreased the ADG and exposure to 35 ppm decreased the ADG, ADFI, and F/G. Furthermore, the increase in head-shaking frequency after 2 h and 24 h ammonia exposure was significantly associated with production performance and lung tissue injury after 21 d ammonia exposure. In conclusion, the head-shaking behavior at the initial stage of ammonia exposure can reflect the degree of harm of the later production performance and lung tissue health.
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http://dx.doi.org/10.1016/j.psj.2020.10.067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7936116PMC
March 2021

A Virus-Mimicking Nucleic Acid Nanogel Reprograms Microglia and Macrophages for Glioblastoma Therapy.

Adv Mater 2021 Mar 27;33(9):e2006116. Epub 2021 Jan 27.

School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200 240, China.

Immunotherapy is recognized as one of the most promising approaches to treat cancers. However, its effect in glioblastoma (GBM) treatment is insufficient, which can in part be attributed to the immunosuppressive tumor microenvironment (TME). Microglia and macrophages are the main immune infiltrating cells in the TME of GBM. Unfortunately, instead of initiating the anti-tumor response, GBM-infiltrating microglia and macrophages switch to a tumor-promoting phenotype (M2), and support tumor growth, angiogenesis, and immunosuppression by the release of cytokines. In this work, a virus-mimicking membrane-coated nucleic acid nanogel Vir-Gel embedded with therapeutic miRNA is developed, which can reprogram microglia and macrophages from a pro-invasive M2 phenotype to an anti-tumor M1 phenotype. By mimicking the virus infection process, Vir-Gel significantly enhances the targetability and cell uptake efficiency of the miR155-bearing nucleic acid nanogel. In vivo evaluations demonstrate that Vir-Gel apparently prolongs the circulation lifetime of miR155 and endows it with an active tumor-targeting capability and excellent tumor inhibition efficacy. Owing to its noninvasive feature and effective delivery capability, the virus-mimicking nucleic acid nanogel provides a general and convenient platform that can successfully treat a wide range of diseases.
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http://dx.doi.org/10.1002/adma.202006116DOI Listing
March 2021

The Roles of the miRNAome and Transcriptome in the Ovine Ovary Reveal Poor Efficiency in Juvenile Superovulation.

Animals (Basel) 2021 Jan 19;11(1). Epub 2021 Jan 19.

Institute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China.

Juvenile superovulation can provide a wealth of oocyte material for embryo production, animal cloning, and genetic modification research, but embryos derived from juvenile oocytes show poor efficiency in subsequent developmental capacity. In order to reveal the formation mechanism of large numbers of follicles and poor oocyte quality in juvenile ovaries under superovulation treatment, differentially expressed microRNAs (miRNAs) and messenger RNAs (mRNAs) were characterized and investigated in the ovaries of lambs and adult sheep using high-throughput sequencing technology. The majority of differentially expressed miRNAs (337/358) were upregulated in lamb libraries. The expression levels of mRNAs related to hormone receptors (follicle-stimulating hormone receptor, ; luteinizing hormone/choriogonadotropin receptor, ; estrogen receptor 1, ), steroid hormone secretion (cytochrome P450 family 11 subfamily A member 1, ; cytochrome P450 family 17 subfamily A member 1, ; cytochrome P450 family 19 subfamily A member 1, ), and oocyte quality (pentraxin 3, ; BCL2 apoptosis regulator, ; caspase 3, ) were significantly different between the lamb and adult libraries. The miRNA aor-miR-143, which targets , was highly and differentially expressed, and was predicted to be targeted by oar-miR-485-3p and oar-miR-377-3p in the ovine ovary. A considerable number of miRNAs were predicted to inhibit expression in lamb ovaries. In conclusion, oar-miR-143 and molecules, among others, might regulate follicle formation, and oar-miR-485-3p, oar-miR-377-3p, and , among others, may be associated with oocyte quality. These identified miRNAs and mRNAs will be beneficial for the prediction of ovarian superovulation potential and screening of oocytes.
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http://dx.doi.org/10.3390/ani11010239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7832859PMC
January 2021

Modified Simiaowan prevents articular cartilage injury in experimental gouty arthritis by negative regulation of STAT3 pathway.

J Ethnopharmacol 2021 Apr 15;270:113825. Epub 2021 Jan 15.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China. Electronic address:

Ethnopharmacological Relevance: Modified Simiaowan (MSW) is a traditional Chinese medicine formula that is composed of six herbs. It has been widely used in the treatment of gouty arthritis.

Aim Of The Study: This study was designed to investigate the effect of MSW on gouty arthritis and explore the possible mechanisms.

Material And Methods: The rat gouty arthritis model was established by intra-articular injection of Monosodium Urate (MSU) crystal, and then treated with MSW for 5 days. The perimeter of the knee joints was measured in a time-dependent manner and serum samples were collected for the detection of TNF-α, IL-1β, and IL-6 protein levels by ELISA. The protein expressions of MMP-3, TIMP-3, STAT3, and p-STAT3 in cartilage tissues and C28/I2 cells were detected by Western blot, and the levels of proteoglycan in primary chondrocytes and cartilage tissues were determined by toluidine blue staining. In addition, AG490 and IL-6 were used in vitro to explore the function of IL-6/STAT3 pathway in the protective effect of MSU.

Results: MSW reduced the joint swelling rate in gouty arthritis model and inhibited MSU induced up-regulation of IL-1β, TNF-α, and IL-6 protein levels in serum and synovial fluid. IL-1β induced an increase in p-STAT3 and MMP-3 protein expression in C28/I2 cells, as well as a decrease in TIMP-3. MSW serum inhibited the protein expression changes induced by IL-1β in vitro. Furthermore, inhibition of STAT3 signaling negated the effect of MSW serum on p-STAT3, MMP-3, and TIMP-3 protein levels in C28/I2 cells. MSW also increased the content of proteoglycan significantly both in vivo and in vitro.

Conclusion: Our data indicated that MSW protected rats from MSU-induced experimental gouty arthritis and IL-1β/IL-6/STAT3 pathway played an essential role in the protective effect of MSU against GA.
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http://dx.doi.org/10.1016/j.jep.2021.113825DOI Listing
April 2021

Using 3-Dimensional Motion Analysis to Optimize Treatment Planning for a Patient With Dropfoot: Case Report.

Phys Ther 2021 Mar;101(3)

Motion Analysis Center, Shriners Hospitals for Children, Portland, OR 97239, USA.

Objective: This study demonstrated the use of computerized motion analysis to assist in evidence-based clinical decision-making.

Case Description: A 15-year-old girl who had right hemiparesis after a stroke was referred for 3-dimensional computerized motion analysis to determine the effect of 3 devices intended to control her dropfoot and to assist in developing a treatment plan. Four conditions were tested and compared: barefoot, lateral support ankle brace, functional electrical stimulation (FES) device, and dropfoot cuff.

Results: Kinematics showed the right ankle had significant dropfoot during swing phase (32.7 degrees of plantarflexion at terminal swing) in barefoot. The lateral support ankle brace, FES device, and dropfoot cuff reduced terminal swing plantarflexion to 27.2 degrees, 17.6 degrees, and 15.3 degrees, respectively, though ankle kinematics remained abnormal because of inadequate dorsiflexion. Improvements in gait variable score with FES (-8.2 degrees) or dropfoot cuff (-8.7 degrees) were significantly more than that with the lateral support brace (-2.2 degrees), and the difference in gait variable score between FES and dropfoot cuff was insignificant. Compared with the barefoot condition, the lateral support brace condition did not show a clinically significant difference in gait profile score; however, the gait profile scores of both FES and dropfoot cuff conditions showed clinically significant improvement (-1.7 degrees and -2.1 degrees, respectively).

Conclusion: Objective data delineated subtle changes among 3 devices and led to the recommendation to discontinue the lateral support ankle brace, continue using her night ankle-foot orthosis and FES device, with the dropfoot cuff as a backup when she feels leg fatigue or skin irritation, and consider serial casting or surgical calf lengthening.

Impact: Computerized motion analysis provides quantitative evaluation of subtle differences in the effect of braces with different designs, which are hard for the human eye to discern. The objective data inform and validate treatment decision-making. The recommendations were made as a result of evidence-based practice.
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http://dx.doi.org/10.1093/ptj/pzab003DOI Listing
March 2021