Publications by authors named "Jing Dong"

822 Publications

Microscopic Mass Spectrometry Imaging Reveals the Distribution of Phytochemicals in the Dried Root of .

Front Pharmacol 2021 29;12:685575. Epub 2021 Jun 29.

Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

The dried root of L. (Brassicaceae) is one of the most popular traditional Chinese medicines with well-recognized prevention and treatment effects against viral infections. Above 300 components have been isolated from this herb, but their spatial distribution in the root tissue remains unknown. In recent years, mass spectrometry imaging (MSI) has become a booming technology for capturing the spatial accumulation and localization of molecules in fresh plants, animal, or human tissues. However, few studies were conducted on the dried herbal materials due to the obstacles in cryosectioning. In this study, distribution of phytochemicals in the dried root of was revealed by microscopic mass spectrometry imaging, with application of atmospheric pressure-matrix-assisted laser desorption/ionization (AP-MALDI) and ion trap-time-of-flight mass spectrometry (IT-TOF/MS). After optimization of the slice preparation and matrix application, 118 ions were identified without extraction and isolation, and the locations of some metabolites in the dried root of were comprehensively visualized for the first time. Combining with partial least square (PLS) regression, samples collected from four habitats were differentiated unambiguously based on their mass spectrometry imaging.
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http://dx.doi.org/10.3389/fphar.2021.685575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276017PMC
June 2021

Association between plasma endothelial microparticles and contrast-induced nephropathy in patients underwent coronary angiography.

Medicine (Baltimore) 2021 Jul;100(28):e24004

Department of Cardiology, Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.

Abstract: We aim to investigate the association between plasma endothelial microparticles (EMPs) and contrast-induced nephropathy of patients underwent coronary angiography.The patients were divided into normal renal function group and renal dysfunction group based on the estimated glomerular filtration rate (eGFR). Among the 180 cases, 117 received determination of EMP and serum creatinine after percutaneous coronary intervention (PCI) and/or coronary angiography. The patients were divided into contrast-induced-nephropathy (CIN) group and non-CIN group. EMPs collection and determination were performed, together with biochemical analysis and digital subtraction angiography (DSA) analysis.Spearman correlation showed that the expression of EMP was negatively correlated with eGFR (r = -0.201, P < .01). The serum hypersensitive C-reactive protein (hs-CRP), cystatin C (Cys-C), uric acid (UA) were significantly higher in CIN group than that in the non CIN group. Spearman correlation showed that the expression of EMP was positively correlated with serum interleukin-6 (IL-6, r = 0.393, P < .01). The expression of EMP was positively correlated with serum hs-CRP (r = 0.360, P < .01). Logistic regression analysis showed that the levels of N-terminal pro-brain natriuretic peptide (NT-proBNP), eGFR, UA, and Cys-C were correlated with the incidence of contrast induced nephropathy.In patients with contrast-induced-nephropathy, the plasma EMPs were significantly increased after coronary angiography. The expression of plasma EMPs may play a role in the occurrence of contrast-induced-nephropathy.
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http://dx.doi.org/10.1097/MD.0000000000024004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284766PMC
July 2021

Zebrafish Model for Screening Antiatherosclerosis Drugs.

Oxid Med Cell Longev 2021 22;2021:9995401. Epub 2021 Jun 22.

School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198 Jiangsu, China.

This study is aimed at establishing a zebrafish model of AS, which can be applied for high-throughput screening anti-AS drugs. A zebrafish AS model was induced by high cholesterol diet (HCD) and lipopolysaccharide (LPS). In the early stage of modeling, HCD induced zebrafish to show some early symptoms similar to human AS, mainly cholesterol accumulation, vascular inflammation, lipid metabolism disorder, and oxidative stress. In addition to lipid metabolism disorders, LPS also induced the same symptoms. And when HCD and LPS exist at the same time, these AS symptoms in zebrafish become more severe. When the modeling time reached 45 days, HCD and LPS induce the formation of plaques in zebrafish blood vessels, and these plaques contain fibrous tissue and lipids, which are similar to human AS plaques. We also evaluated the efficacy of some anti-AS drugs (atorvastatin, aspirin, and vitamin C) through these zebrafish AS models. The results found that atorvastatin can significantly reduce the symptoms of AS induced by HCD and LPS, and aspirin and vitamins can significantly reduce the symptoms of AS induced by LPS. It is feasible to use zebrafish to establish an AS model, and the zebrafish AS model can be used for high-throughput screening of anti-AS drugs.
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http://dx.doi.org/10.1155/2021/9995401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245221PMC
June 2021

Electrocatalytic ethylbenzene valorization using a [email protected] triazine framework with water as the oxygen source.

Chem Commun (Camb) 2021 Jul;57(60):7430-7433

Key Laboratory of Cluster Science Ministry of Education, Beijing Key Laboratory of Photoelectroic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.

Ethylbenzene (EB) oxidation is an important transformation in the chemical industry. Herein, [email protected], a noble metal free electrocatalyst, was used to promote the oxidative upgrading of EB. Under ambient conditions, 65% of EB was converted to three value-added products using water as the oxygen source yielding a total Faraday efficiency of 90.4%. This excellent performance is ascribed to the homogeneous dispersion of PMo10V2 and its dual role in the electrocatalytic process.
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http://dx.doi.org/10.1039/d1cc03186dDOI Listing
July 2021

The effects of bariatric surgery on dyslipidemia and insulin resistance in overweight patients with or without type 2 diabetes: a systematic review and network meta-analysis.

Surg Obes Relat Dis 2021 Apr 14. Epub 2021 Apr 14.

Special Medicine Department, Medical College, Qingdao University, Qingdao, China; Physiology Department, Medical College, Qingdao University, Qingdao, China. Electronic address:

Obesity has become an epidemic in several regions globally; it may lead to cardiovascular diseases, diabetes, and dyslipidemia. Despite many therapies, all bariatric procedures fail in some patients. There is a lack of literature comparing treatment effects on specific metabolic indexes. PubMed, Embase, and Cochrane Central Register of Controlled Trials were searched for relevant articles. GeMTC and R software were used to perform a network meta-analysis, draw forest plots, investigate the possibility of statistical heterogeneity, generate I statistics, rank probabilities, and evaluate relative effects of surgical procedures. All analyses were based on a Bayesian consistency model. We included 35 randomized controlled trials, comprising 2198 individuals and 13 interventions. For patients with high insulin resistance, single-anastomosis (mini-) gastric bypass (SAGB) and sleeve gastrectomy (SG) may be effective options, with mean differences (95% confidence intervals [CIs]) of -4.45 (-9.04 to -.34) and -4.23 (-6.74 to -2.22), respectively, compared with control groups. For patients with severe dyslipidemia, in addition to SAGB and SG, duodenal switch (DS) may be an effective surgery, with mean differences (95% CIs) of -.97 (-1.39 to -.55), -1.98 (-3.76 to -.19), .53 (.04 to 1.04), and -.94 (-1.66 to -.16) compared with control groups in terms of triglycerides, total cholesterol, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) concentrations, respectively. In adult overweight patients with or without diabetes, SAGB and SG are most effective at ameliorating insulin resistance. SAGB, Roux-en-Y gastric bypass + omentectomy, and DS are useful for reducing triglycerides, total cholesterol, and LDL-C. SG + omentectomy elevates HDL-C concentrations best. Adjustable gastric band and biliopancreatic diversion may not control insulin resistance or dyslipidemia well.
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http://dx.doi.org/10.1016/j.soard.2021.04.005DOI Listing
April 2021

The chromosome-level genome of Triplophysa dalaica (Cypriniformes: Cobitidae) provides insights into its survival in extremely alkaline environment.

Genome Biol Evol 2021 Jun 29. Epub 2021 Jun 29.

College of Fisheries, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, Henan Normal University, Xinxiang 453007, Henan, China.

Lake Dali Nur, located in Inner Mongolia, North China, is alkaline, with Triplophysa dalaica one of the three fish species that not only survive, but thrive, in the lake. To investigate the presence of molecular mutations potentially responsible for this adaptation, the whole genome sequence of the species was sequenced. A total of 126.5 Gb and 106 Gb data, covering nearly 200X of the estimated genome, were generated using long-read sequencing and Hi-C technology, respectively. De novo assembly generated a genome totalled 607.91 Mb, with a contig N50 of 9.27 Mb. Nearly all whole genome sequences were anchored and oriented onto 25 chromosomes, with telomeres for most chromosomes also being recovered. Repeats comprised approximately 35.01% of the whole genome. A total of 23,925 protein-coding genes were predicted, within which, 98.62% could be functionally annotated. Through comparisons of T. dalaica, T. tibetana, and T. siluroides gene models, a total of 898 genes were identified as likely being subjected to positive selection, with several of them potentially associated with alkaline adaptation, such as sodium bicarbonate cotransporter, SLC4A4. Demographic analyses suggested that the Dali population might have diverged from endemic freshwater Hai River populations, approximately 1 million years ago. The high-quality T. dalaica genome, created in this study, not only aids in the analyses of alkaline adaptation, but may also assist in revealing the mysteries of the highly divergent genus Triplophysa in the future.
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http://dx.doi.org/10.1093/gbe/evab153DOI Listing
June 2021

Reduced polyoxomolybdate immobilized on reduced graphene oxide for rapid catalytic decontamination of a sulfur mustard simulant.

Dalton Trans 2021 Jul;50(28):9796-9803

Key Laboratory of Cluster Science Ministry of Education, Beijing Key Laboratory of Photoelectroic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488, People's Republic of China.

Keggin-type polyoxometalates (POMs) were immobilized on poly(diallyldimethylammonium chloride) (PDDA) functionalized reduced graphene oxide (rGO) by a facile and broad-spectrum hydrothermal method. The prepared [email protected] composites (POM = H3PMo12O40, H3PW12O40, H5PMo10V2O40) have been thoroughly characterized using a series of techniques. The three composites can catalyze the oxidative decontamination of a sulfur mustard simulant, 2-chloroethyl ethyl sulfide (CEES) in the order of [email protected] > [email protected] > [email protected] Notably, under ambient conditions [email protected] can convert 99% of CEES within 30 min in the presence of nearly stoichiometric aqueous H2O2 (3 wt%) and its catalytic activity is significantly higher than that of homogeneous H3PMo12O40. XPS spectral analysis and control experiments indicate that the Mo center of POM is reduced from +6 to +5 during the hydrothermal process, and the excellent catalytic performance is related to the reduction of Mo. Moreover, the [email protected] composite is stable during the decontamination process and it can be used for at least five cycles without loss of activity.
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http://dx.doi.org/10.1039/d1dt01265gDOI Listing
July 2021

Cobalt-Phthalocyanine-Derived Molecular Isolation Layer for Highly Stable Lithium Anode.

Angew Chem Int Ed Engl 2021 Jun 27. Epub 2021 Jun 27.

Center Énergie Matériaux et Télécommunications, Institut National de la Recherche Scientifique (INRS), Varennes, Québec, J3X 1S2, Canada.

The uneven consumption of anions during the lithium (Li) deposition process triggers a space charge effect that generates Li dendrites, seriously hindering the practical application of Li-metal batteries. We report on a cobalt phthalocyanine electrolyte additive with a planar molecular structure, which can be tightly adsorbed on the Li anode surface to form a dense molecular layer. Such a planar molecular layer cannot only complex with Li ions to reduce the space charge effect, but also suppress side reactions between the anode and the electrolyte, producing a stable solid electrolyte interphase composed of amorphous lithium fluoride (LiF) and lithium carbonate (LiCO ), as verified by X-ray absorption near-edge spectroscopy. As a result, the Li|Li symmetric cell exhibits excellent cycling stability above 700 h under a high plating capacity of 3 mAh cm . Moreover, the assembled Li|lithium iron phosphate (LiFePO , LFP) full-cell can also deliver excellent cycling over 200 cycles under lean electrolyte conditions (3 μL mg ).
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http://dx.doi.org/10.1002/anie.202106027DOI Listing
June 2021

The complete mitochondrial genome sequence of (Rosaceae).

Mitochondrial DNA B Resour 2021 Jun 14;6(7):1951-1952. Epub 2021 Jun 14.

Beijing Academy of Forestry and Pomology Sciences, Beijing, P.R. China.

Lozinsk. is valuable germplasm material for cross breeding in . In this study, we assembled the complete mitochondrial genome of using a combination of Illumina data and Nanopore data. The mitochondrial genome was 275,143 bp in length, including 29 protein-coding genes, 20 tRNA genes, and three rRNA genes, with a total GC content 45.23%. Seven protein-coding genes contained introns, and three were trans-spliced. Phylogenetic analysis indicated that is making a sister clade to the Amygdaloideae species. The complete mitochondrial genome of reported in this study will improve our understanding of evolution.
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http://dx.doi.org/10.1080/23802359.2021.1920498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204990PMC
June 2021

Temperature-Dependent Residue Depletion Regularities of Tiamulin in Nile Tilapia () Following Multiple Oral Administrations.

Front Vet Sci 2021 11;8:679657. Epub 2021 Jun 11.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.

The aim of this study was to investigate the effect of different water temperatures (19, 25, and 30°C) on tissue residue depletion of tiamulin in Nile tilapia () after five consecutive days of oral administration at the dose of 20 mg/kg body weight and to calculate the corresponding elimination half-life ( ) and withdrawal times (WTs). After oral administration at scheduled 11 time points (1, 2, 3, 5, 7, 9, 12, 15, 20, 25, and 30 days), samples of plasma and tissues (muscle plus skin, liver, kidney, and gill) were collected. Tiamulin concentration in samples were determined by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). was calculated by the equation: = ln2/. WT 1.4 software was used to calculate WT. The results showed that tiamulin was widely distributed in all tissue samples with the highest concentration in liver. At three different water temperatures, the were calculated as 2.76, 2.13, and 1.64 days in plasma, 2.71, 1.85, and 1.31 days in muscle plus skin, 2.27, 1.70, and 1.50 days in liver, 2.84, 2.32, and 1.94 day in kidney, and 3.16, 2.42, and 1.74 days in gill, respectively. At 19°C, the order of WT is kidney (11.88 days) > liver (10.41 days) > gill (10.77 days) > plasma (8.83 days) > muscle plus skin (7.14 days). The WT for tiamulin at 25°C was in the following order: kidney (8.40 days) > liver (8.21 days) > gill (8.07 days) > plasma (7.24 days) > muscle plus skin (4.05 days). At 30°C, the WT dropped and shown as follows: gill (6.99 days) > kidney (6.51 days) > liver (6.29 days) > plasma (3.27 days) > muscle plus skin (2.92 days). The present investigations indicated that increasing the temperature from 19 to 30°C shortened and WT of tiamulin in tilapia. To ensure the safety of fish consumption, the longest WT of tissues is suggested for tiamulin in Nile tilapia at the corresponding water temperature; i.e., WTs were 12 days at 19°C, 9 days at 25°C, and 7 days at 30°C, respectively. Overall, we intended to provide a theoretical basis for tissue residue depletion kinetics of tiamulin in fish and improve our understanding of the influence of the temperature on tissue residue depletion kinetics of tiamulin in fish.
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http://dx.doi.org/10.3389/fvets.2021.679657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225925PMC
June 2021

Immunomodulatory effects of Nigella sativa seed polysaccharides by gut microbial and proteomic technologies.

Int J Biol Macromol 2021 Aug 22;184:483-496. Epub 2021 Jun 22.

National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, China; Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng 475004, Henan, China; Functional Food Engineering Technology Research Center, Kaifeng 475004, Henan, China. Electronic address:

Cyclophosphamide (CTX) was used to establish the immunosuppressive mice model. The immune organ viscera index, phagocytes vitality, the levels of cytokines in serum, the oxidative stress resistance, proteomics and intestinal flora in mice were investigated to evaluate the effect of immune regulation of Nigella sativa seed polysaccharide (NSSP). The results showed that the high-dose NSSP group could significantly increase the thymus and spleen index. The levels of ACP, LDH, T-AOC, SOD, IL-2, IL-4 and IL-6 were significantly increased and the levels of TNF-α and MDA were reduced. All evidences indicated that NSSP could improve the immune effects of the immunosuppressed mice. Proteomics investigation showed that NSSP could improve the immune by regulating the differential proteins of PI3K and PTEN, and regulating the metabolism-related pathways such as autoimmune diseases and PI3K-Akt signaling pathway. of Gut microbes analysis showed that NSSP could exert immunomodulatory effects by improving the structure of the intestinal flora, increasing the diversity of the flora, and regulating metabolic pathways such as lipid metabolism, polysaccharide synthesis and signal transduction by the prediction of flora metabolic functions. In addition, NSSP could regulate intestinal environment by regulating the content of short chain fatty acids.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.118DOI Listing
August 2021

Single-cell RNA sequencing reveals the mechanism of sonodynamic therapy combined with a RAS inhibitor in the setting of hepatocellular carcinoma.

J Nanobiotechnology 2021 Jun 12;19(1):177. Epub 2021 Jun 12.

Department of Ultrasound, Harbin Medical University Cancer Hospital, No.150, Haping Road, Nangang District, Harbin, 150081, Heilongjiang, China.

Background: Ras activation is a frequent event in hepatocellular carcinoma (HCC). Combining a RAS inhibitor with traditional clinical therapeutics might be hampered by a variety of side effects, thus hindering further clinical translation. Herein, we report on integrating an IR820 nanocapsule-augmented sonodynamic therapy (SDT) with the RAS inhibitor farnesyl-thiosalicylic acid (FTS). Using cellular and tumor models, we demonstrate that combined nanocapsule-augmented SDT with FTS induces an anti-tumor effect, which not only inhibits tumor progression, and enables fluorescence imaging. To dissect the mechanism of a combined tumoricidal therapeutic strategy, we investigated the scRNA-seq transcriptional profiles of an HCC xenograft following treatment.

Results: Integrative single-cell analysis identified several clusters that defined many corresponding differentially expressed genes, which provided a global view of cellular heterogeneity in HCC after combined SDT/FTS treatment. We conclude that the combination treatment suppressed HCC, and did so by inhibiting endothelial cells and a modulated immunity. Moreover, hepatic stellate secretes hepatocyte growth factor, which plays a key role in treating SDT combined FTS. By contrast, enrichment analysis estimated the functional roles of differentially expressed genes. The Gene Ontology terms "cadherin binding" and "cell adhesion molecule binding" and KEGG pathway "pathway in cancer" were significantly enriched by differentially expressed genes after combined SDT/FTS therapy.

Conclusions: Thus, some undefined mechanisms were revealed by scRNA-seq analysis. This report provides a novel proof-of-concept for combinatorial HCC-targeted therapeutics that is based on a non-invasive anti-tumor therapeutic strategy and a RAS inhibitor.
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http://dx.doi.org/10.1186/s12951-021-00923-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199394PMC
June 2021

Dual-Functional Ti(IV)-IMAC Material Enables Simultaneous Enrichment and Separation of Diverse Glycopeptides and Phosphopeptides.

Anal Chem 2021 06 8;93(24):8568-8576. Epub 2021 Jun 8.

School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.

Simultaneous enrichment and fractionation of diverse proteins/peptides possessing different post-translational modifications (PTMs) from the same biological samples is highly desirable to reduce sample consumption, avoid complicated sample processing, and enable studies of potential crosstalks between different PTMs. In this work, we report a new approach to enable simultaneous enrichment and separation of glycopeptides, phosphopeptides, and mannose-6-phosphate (M6P) glycopeptides by using a dual-functional Ti(IV)-IMAC material. Moreover, we also made the separation of neutral and sialyl glycopeptides and mono- and multi-phosphopeptides possible by performing different elution processes according to the differences in their electrostatic or hydrophilic properties. These separations are effective and efficient to eliminate the signal suppression from neutral glycopeptides for sialyl glycopeptide detection, allowing separation of mono-phosphopeptides from multi-phosphopeptides, as well as detection of M6P glycopeptides that are free from the abovementioned modifications. This new strategy significantly improves the coverage and identification numbers of glycopeptides, phosphopeptides, and M6P glycopeptides by 1.9, 2.3, and 4.3-fold compared with the conventional method, respectively. This is the first report on simultaneous enrichment and separation of neutral and sialyl glycopeptides, mono- and multi-phosphopeptides, and M6P glycopeptides via dual-functional Ti(IV)- IMAC, revealing novel insights into potential crosstalk among these important PTMs.
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http://dx.doi.org/10.1021/acs.analchem.1c01324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245321PMC
June 2021

In situ Raman enhancement strategy for highly sensitive and quantitative lateral flow assay.

Anal Bioanal Chem 2021 Jun 5. Epub 2021 Jun 5.

Key Laboratory for Chemical Biology of Fujian Province, MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, Fujian, China.

As a paper-based analytical platform, lateral flow assay (LFA) gets benefit from the rapid analysis, low cost, high selectivity, good stability, and user-friendliness, and thus has been widely used in rapid screening or assisted diagnosis. Nevertheless, LFA still suffers from low detection sensitivity via the naked eye, limiting its applications to qualitative and semi-quantitative tests. To enhance the signal readout, various nanoparticle signal tags have been employed to replace traditional colloidal gold nanoparticles (AuNPs), such as fluorescent nanoparticles (FNPs), magnetic nanoparticles (MNPs), and Raman reporter-labeled nanoparticles. In particular, Raman reporter-labeled nanoparticles are extremely sensitive due to remarkable signal enhancement effect on metal surface. However, the application of LFA is still hampered by the poor stability of Raman reporter-labeled nanoparticles. Herein, we developed an in situ Raman enhancement strategy to create a surface-enhanced Raman scattering (SERS) signal on the AuNPs, shortened as "i-SERS," which not only preserves the original advantages of the colloidal gold strip (AuNPs-LFA), but also realizes highly sensitive and quantitative detection. We applied the i-SERS for procalcitonin (PCT) detection. The experimental process takes only 16 min, and the limit of detection (LOD) is 0.03 ng mL, far below the value using AuNPs-LFA. These results indicate that i-SERS assay was highly sensitive and suitable for the rapid detection of PCT.
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http://dx.doi.org/10.1007/s00216-021-03419-zDOI Listing
June 2021

De novo TCOF1 mutation in Treacher Collins syndrome.

Int J Pediatr Otorhinolaryngol 2021 Aug 11;147:110765. Epub 2021 May 11.

Rare Disease Center, Shulan Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College, Hangzhou, 310000, China. Electronic address:

Objective: To analyze the genetic cause of a hearing loss child with the Treacher Collins syndrome (TCS) phenotypes of malar hypoplasia, micrognathia, antimongoloid slanting palpebral fissures and cup ears.

Methods: Clinical analysis, hearing tests, chromosomal microarray analysis (CMA) and whole exome sequencing (WES) were performed on the family members.

Results: The 6 months old boy with a range of Treacher Collins syndrome phenotypes including malar hypoplasia, micrognathia, antimongoloid slanting palpebral fissures, cup ears, and hearing loss. While CMA analyses did not detect significant deletion or duplication, WES analysis identified a novel nonsense mutation c.163C > T (p.Q55X) in exon 2 of TCOF1 gene. Sanger sequencing analysis confirmed the mutation in the patient, but not in his parents.

Conclusion: This article reports a novel nonsense mutation located at exon 2 in TCOF1 gene, which predicts premature protein termination of treacle, indicating that haploinsufficiency of TCOF1 gene is responsible for Treacher Collins syndrome. Our study increases the cohort of Chinese TCS patients, and expands the TCS variation spectrum.
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http://dx.doi.org/10.1016/j.ijporl.2021.110765DOI Listing
August 2021

Blood flow changes in the forearm arteries after ultrasound-guided costoclavicular brachial plexus blocks: a prospective observational study.

BMC Anesthesiol 2021 May 29;21(1):164. Epub 2021 May 29.

Department of Anesthesiology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai, China.

Background: An increase in blood flow in the forearm arteries has been reported after brachial plexus block (BPB). However, few studies have quantitatively analysed the blood flow of the forearm arteries after BPB or have studied only partial haemodynamic parameters. The purpose of the present study was to comprehensively assess blood flow changes in the distal radial artery (RA) and ulnar artery (UA) after BPB performed via a new costoclavicular space (CCS) approach using colour Doppler ultrasound.

Methods: Thirty patients who underwent amputated finger replantation and received ultrasound-guided costoclavicular BPB were included in the study. The haemodynamic parameters of the RA and UA were recorded before the block and 10 min, 20 min, and 30 min after the block using colour Doppler ultrasound to determine the peak systolic velocity (PSV), end-diastolic velocity (EDV), mean velocity (V), pulsatility index (PI), resistance index (RI) and area. The volumetric flow rate (VFR) was calculated using the formula Q = area×V. The aforementioned parameters were compared not only before and after the BPB but also between the RA and UA.

Results: Compared with those of the respective baselines, there was a significant increase in the PSV, EDV, V, area, and VFR and a significant decrease in the PI and RI of the RA and UA 10 min, 20 min, and 30 min post-block. The increase 30 min post-block in EDV (258.68 % in the RA, 279.63 % in the UA) was the most notable, followed by that in the V (183.36 % in the RA, 235.24 % in the UA), and the PSV (139.11 % in the RA, 153.15 % in the UA) changed minimally. The V and VFR of the RA were significantly greater than those of the UA before the BPB; however, there was no significant difference in the VFR between the RA and UA after the BPB.

Conclusions: A costoclavicular BPB can increase blood flow in the forearm arteries. The RA had a higher volumetric flow rate than the UA before the BPB; however, the potential blood supply capacity of the UA was similar to that of the RA after a BPB.

Trial Registration: This study was registered at the Chinese Clinical Trial Registry (http://www.chictr.org.cn/searchproj.aspx, clinical trial number: ChiCTR 1900023796, date of registration: June 12, 2019).
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http://dx.doi.org/10.1186/s12871-021-01383-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164273PMC
May 2021

Myeloid-derived growth factor inhibits inflammation and alleviates endothelial injury and atherosclerosis in mice.

Sci Adv 2021 May 21;7(21). Epub 2021 May 21.

Department of Endocrinology, General Hospital of Central Theater Command, Wuluo Road 627, Wuhan 430070, Hubei Province, China.

Whether bone marrow modulates systemic metabolism remains unknown. Here, we found that (i) myeloid cell-specific myeloid-derived growth factor (MYDGF) deficiency exacerbated vascular inflammation, adhesion responses, endothelial injury, and atherosclerosis in vivo. (ii) Myeloid cell-specific MYDGF restoration attenuated vascular inflammation, adhesion responses and leukocyte homing and alleviated endothelial injury and atherosclerosis in vivo. (iii) MYDGF attenuated endothelial inflammation, apoptosis, permeability, and adhesion responses induced by palmitic acid in vitro. (iv) MYDGF alleviated endothelial injury and atherosclerosis through mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4)/nuclear factor κB (NF-κB) signaling. Therefore, we concluded that MYDGF inhibits endothelial inflammation and adhesion responses, blunts leukocyte homing, protects against endothelial injury and atherosclerosis in a manner involving MAP4K4/NF-κB signaling, and serves as a cross-talk factor between bone marrow and arteries to regulate the pathophysiology of arteries. Bone marrow functions as an endocrine organ and serves as a potential therapeutic target for metabolic disorders.
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http://dx.doi.org/10.1126/sciadv.abe6903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139583PMC
May 2021

(-)-Epicatechin acts as a potent agonist of the membrane androgen receptor, ZIP9 (SLC39A9), to promote apoptosis of breast and prostate cancer cells.

J Steroid Biochem Mol Biol 2021 Jul 11;211:105906. Epub 2021 May 11.

Marine Science Institute, University of Texas at Austin, 750 Channel View Drive, Port Aransas, TX, 78373, United States.

(-)-Epicatechin, a flavonoid present in high concentrations in foods such as green tea and cocoa, exerts beneficial and protective effects in numerous disease models, including anti-tumorigenesis and apoptosis in human breast and prostate cancer cells. Potential interactions of (-)-epicatechin and (+)-catechin with the membrane androgen receptor, ZIP9 (SLC39A9), which mediates androgen induction of apoptosis in these cancer cells, were investigated. Both (-)-epicatechin and (+)-catechin were effective competitors of [H]-testosterone binding to PC-3 prostate cancer cells (nuclear androgen receptor-negative, nAR-null) overexpressing ZIP9 (PC3-ZIP9), with relative binding affinities of 75 % and 28 % that of testosterone, respectively. (-)-Epicatechin (200 nM) mimicked the effects of 100 nM testosterone in inducing apoptosis of PC3-ZIP9 cells, whereas (+)-catechin (concentration range 200 nM-1000 nM) did not significantly increase apoptosis and instead blocked the apoptotic response to testosterone. (-)-Epicatechin also activated androgen-dependent ZIP9 signaling pathways, inducing decreases in cAMP production and elevating intracellular free zinc levels, while (+)-catechin typically lacked these actions. Both (-)-epicatechin and (+)-catechin also bound to cell membranes of MDA-MB-468 breast cancer cells (nAR-null, high ZIP9 expression). MDA-MB-468 cells showed similar apoptotic, cAMP, and free zinc signaling responses to (-)-epicatechin to those observed in PC3-ZIP9 cells, as well as antagonism by (+)-catechin of testosterone-induced apoptosis and modulation of cAMP and caspase-3 levels. Moreover, knockdown of ZIP9 expression in MDA-MB-468 cells with siRNA decreased specific [H]-testosterone binding of both catechins and blocked the apoptotic and free zinc responses to testosterone and (-)-epicatechin. The results indicate (-)-epicatechin is a potent ZIP9 agonist in breast and prostate cancer cells.
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http://dx.doi.org/10.1016/j.jsbmb.2021.105906DOI Listing
July 2021

Mesoporous NiCo alloy/reduced graphene oxide nanocomposites as efficient hydrogen evolution catalysts.

J Colloid Interface Sci 2021 Oct 28;599:603-610. Epub 2021 Apr 28.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address:

Water electrolysis is a clean and efficient route for producing high-purity hydrogen. Developing highly efficient, stable and inexpensive electrocatalysts to replace Pt is currently a major challenge for the widespread application of water splitting. Herein, we report a facile and novel strategy for the synthesis of mesoporous NiCo alloy nanoparticle/reduced graphene oxide (MNiCo/rGO) composites via electroless deposition technique. Owing to the synergistic interaction of Ni and Co, the mesoporous structure of NiCo alloy, and the optimized combination of the mesoporous NiCo with rGO, the obtained optimal MNiCo/rGO catalyst exhibits outstanding electrocatalytic performance for hydrogen evolution reaction (HER) with a low overpotential of 115 mV to reach the current density of 10 mA cm, and a small Tafel slope of 45 mV dec, as well as a high durability in alkaline solution. These remarkable merits make it a favorable alternative to noble metal Pt-based catalysts for HER, thereby further promoting the development of non-noble metal electrocatalysts.
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http://dx.doi.org/10.1016/j.jcis.2021.04.124DOI Listing
October 2021

Antiparasitic Efficacy of Herbal Extracts and Active Compound Against in .

Front Vet Sci 2021 6;8:665072. Epub 2021 Apr 6.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.

spp. Nordmann, 1832 (Monogenea: Gyrodactylidae) are common ectoparasites of teleost fishes. Infection with these parasites can increase the mortality of fish and cause considerable economic losses in intensive aquaculture. To find an effective antiparasitic agent for the control of gyrodactylosis, antiparasitic efficacy of crude extracts of 36 herbal medicines was evaluated using a (Cypriniformes, Cyprinidae)- model. Among all tested medicines, methanol extract of var. (Dioscoreales, Dioscoreaceae) was the most efficient, with an EC value of 4.17 mg/L. This extract showed 100% antiparasitic efficacy against at 10 mg/L and had a therapeutic index (TI, LC/EC) of 5.26, which is higher than that of formaldehyde (TI = 4.58), a widely used parasiticide in aquaculture. Subsequently, the potential mechanism of antiparasitic activity of dioscin, an active compound isolated from var. was investigated and the histopathological alterations in goldfish after exposure to dioscin were also studied. The trial indicated dioscin showed significant antiparasitic activity with a 24 h-EC value of 1.58 mg/L and it exhibited 100% antiparasitic efficacy at 0.6 mg/L. Also, could be completely removed within 2 h at 0.6 mg/L dioscin. Whereas, mean survival time of this worm was 4.99 h, and some individuals even reached 12 h at the same concentration of dioscin. These results indicated that 0.6 mg/L of dioscin did not completely kill all worms within 2 h, but just temporarily remove the worms from goldfish. Scanning electron microscopy (SEM) analysis showed that most of the microvilli on the tegument surface of dropped after exposure to dioscin. This might be one of the potential mechanisms of antiparasitic activity of dioscin against . Furthermore, no severe histopathological alteration was observed after exposure to a high concentration of dioscin for a short time. Considering both effectiveness and safety, therapeutic baths with a high concentration of dioscin for a short time might be a more optimal choice for the treatment of gyrodactylosis in aquaculture.
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http://dx.doi.org/10.3389/fvets.2021.665072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056006PMC
April 2021

Downregulation of microRNA-21-5p from macrophages-derived exosomes represses ventricular remodeling after myocardial infarction via inhibiting tissue inhibitors of metalloproteinase 3.

Int Immunopharmacol 2021 Jul 18;96:107611. Epub 2021 Apr 18.

Cardiovascular Department, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan, China.

Objective: Exosomes are known to transfer microRNAs (miRNAs) to affect the progression of human diseases. We aim to explore the role of M1 macrophages-derived exosomes (M1 exosomes) conveying miR-21-5p in ventricular remodeling in mice with myocardial infarction (MI) by regulating tissue inhibitors of metalloproteinase 3 (TIMP3).

Methods: Macrophages were isolated and co-cultured with miR-21-5p antagomir to extract the exosomes. The modeled mice were injected with relative exosomes to investigate their roles in the cardiac function, pathology of myocardial tissue, myocardial fibrosis, cardiomyocyte apoptosis and ventricular remodeling in MI mice. The expression of miR-21-5p and TIMP3 was detected and their targeting relationship was analyzed.

Results: MiR-21-5p was upregulated while TIMP3 was downregulated in MI mouse myocardial tissues. M1 exosomes impaired cardiac function, aggravated pathology of myocardial tissue, myocardial fibrosis and ventricular remodeling, and promoted cardiomyocyte apoptosis in MI mice. M1 exosomes containing miR-21-5p antagomir alleviated the above alterations, while the role of exosomes containing miR-21-5p antagomir was reversed by silencing TIMP3. TIMP3 was targeted by miR-21-5p.

Conclusion: Downregulation of miR-21-5p from macrophages-derived exosomes suppresses ventricular remodeling after MI via inhibiting TIMP3.
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http://dx.doi.org/10.1016/j.intimp.2021.107611DOI Listing
July 2021

Application of Nano-Insulin Pump in Children with Diabetic Ketoacidosis.

J Nanosci Nanotechnol 2021 10;21(10):5051-5056

Department of Pediatrics, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang, PR China.

Type 1 diabetes is an insulin-dependent type of diabetes that is most common among children. Due to absolute deficiency of insulin in patients, diabetic ketoacidosis (DKA) can easily ensue. Insulin pump can simulate the physiological secretion of islet, but increases the risk of pain and infection in children due to its traumatic effect. This study aimed to analyze the application effect of nano-insulin pump in children with DKA. Children with DKA admitted to our hospital from May 2018 to May 2020 were included in this study and, according to the random number table method, they were divided into two groups, with each group containing 36 cases. The first group received traditional insulin pump infusion (IP), while the second group received nano-insulin pump infusion (NIP). It was found that the reduction of FBG and PBG in NIP group was greater than that in IP group. The recovery time of urine ketone, blood ketone, glucose, venous pH, and other clinical indicators in the NIP group were all lower than those in the IP group ( < 0.05). The length of hospital stay, insulin dosage, incidence of hypoglycemia, and infusion site infection rate in the NIP group were all lower than those in the IP group (P <0.05). The findings indicate that the application of nano-insulin pump in children with DKA had a significant effect and could quickly and obviously correct the levels of blood glucose and ketone body in children.
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http://dx.doi.org/10.1166/jnn.2021.19356DOI Listing
October 2021

Gender differences in risk factors for high plasma homocysteine levels based on a retrospective checkup cohort using a generalized estimating equation analysis.

Lipids Health Dis 2021 Apr 12;20(1):31. Epub 2021 Apr 12.

Beijing Geriatric Healthcare Center, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Xicheng District, Beijing, 100053, China.

Background: Hyperhomocysteinemia (HHcy) is associated with various health problems, but less is known about the gender differences in risk factors for high plasma homocysteine (Hcy) levels.

Methods: In this study, a retrospective study was carried out on 14,911 participants (7838 males and 7073 females) aged 16-102 years who underwent routine checkups between January 2012 and December 2017 in the Health Management Department of Xuanwu Hospital, China. Anthropometric measurements, including body mass index (BMI) and waist-to-hip ratio, were collected. Fasting blood samples were collected to measure the biochemical indexes. The outcome variable was Hcy level, and a generalized estimating equation (GEE) analysis was used to identify the associations of interest based on gender.

Results: Males exhibited increased Hcy levels (16.37 ± 9.66 vs 11.22 ± 4.76 μmol/L) and prevalence of HHcy (37.0% vs 11.3%) compared with females. Hcy levels and HHcy prevalence increased with age in both genders, except for the 16- to 29-year-old group. GEE analysis indicated that irrespective of gender, aspartate aminotransferase, creatinine, uric acid, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol levels were positively correlated with Hcy levels, and alanine aminotransferase, total cholesterol and glucose were negatively correlated with Hcy levels. However, age, BMI and triglycerides (TGs) were positively correlated with Hcy levels exclusively in females.

Conclusions: Gender differences in risk factors for high plasma Hcy levels were noted. Although common correlational factors existed in both genders, age, BMI and TGs were independent risk factors for Hcy levels specifically in females.
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http://dx.doi.org/10.1186/s12944-021-01459-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042914PMC
April 2021

Diversity and community structure of ectomycorrhizal fungi in Pinus thunbergii coastal forests bordering the Yellow Sea of China.

Braz J Microbiol 2021 Jun 4;52(2):801-809. Epub 2021 Apr 4.

Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng, 224002, Jiangsu, China.

Ectomycorrhizas play a fundamental role in the function of forest ecosystems, being essential for plant nutrition absorption and soil quality. Many afforestation and reforestation programmes have begun to recover and maintain coastal forests in China, using pine species including Pinus thunbergii. We investigated the ectomycorrhizal colonization status of P. thunbergii in coastal pine forests of the Yellow Sea of China. We identified a total of 53 ectomycorrhizal fungal species in 74 soil samples collected from three sites and found that Thelephoraceae (10 spp.) and Russulaceae (8 spp.) were the most species-rich ectomycorrhizal fungal lineages. Russula sp. 1 was the most abundant species, accounting for 15.3% of the total ectomycorrhizal tips identified. Most of the remaining species were rare. At this small scale, host identity had no significant effect on the ectomycorrhizal fungal community composition (A = 0.036, P = 0.258), but sampling sites did (A = 0.135, P = 0.041). In addition, Na and K content and soil pH had significant effects on the ectomycorrhizal fungal community. The ectomycorrhizal fungal community associated with different host plants will become an important new direction for research, as ectomycorrhiza may have the potential to improve host capacity to establish in salt-stressed environments. This will provide a theoretical basis and technical support for saline soil reforestation and rehabilitation using pine species with compatible, native ectomycorrhizal fungi in Yellow Sea coastal areas.
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http://dx.doi.org/10.1007/s42770-021-00486-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105453PMC
June 2021

Rheumatoid arthritis coexisting with ankylosing spondylitis: A report of 22 cases with delayed diagnosis.

Medicine (Baltimore) 2021 Apr;100(13):e25051

The Rheumatology and Clinical Immunology Department, the Affiliated Hospital of Qingdao University.

Abstract: Coexisting rheumatoid arthritis (RA) and ankylosing spondylitis (AS) in the same patient is often thought to be rare, and thus misdiagnosis is common. The aim of our study was to describe the main characteristics of RA coexisting with AS in patients with delayed diagnoses and improve awareness of the disease association.Between 2012 and 2018, data from 22 patients who had RA and AS (RA/AS) were retrospectively reviewed. All patients had a history of delayed diagnosis for RA or AS. The clinical features and radiographic changes of RA and AS patients were obtained at baseline and after 2 years. Disease activity score 28 (DAS28) or bath ankylosing spondylitis disease activity index (BASDAI) were used as outcome measures. The mean age at the time of diagnosis of RA/AS was 51.8 years, while the mean duration of diagnostic delay was 5.5 years. Middle-aged women were the most common subgroup among the RA/AS cohort. The common clinical manifestations were systemic, symmetric, peripheral, and axial arthritis. The erythrocyte sedimentation rate and C-reactive protein levels in RA/AS patients were elevated at the time diagnosis of RA/AS. The typical radiologic changes for the 2 diseases coexisted in RA/AS patients. The DAS28 and BASDAI scores at the 2-year follow-up evaluation were lower than the initial assessment.Coexisting RA and AS is often misdiagnosed for many years; a lack of recognition of RA and AS together is one of the most common reasons. Systemic, symmetric, peripheral, and axial arthritis in middle-aged women were the most frequent presentations at onset.
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http://dx.doi.org/10.1097/MD.0000000000025051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021346PMC
April 2021

Use of a Novel Patient-Flow Model to Optimize Hospital Bed Capacity for Medical Patients.

Jt Comm J Qual Patient Saf 2021 Jun 28;47(6):354-363. Epub 2021 Feb 28.

Background: There is no known method for determining the minimum number of beds in hospital inpatient units (IPs) to achieve patient waiting-time targets. This study aims to determine the relationship between patient waiting time-related performance measures and bed utilization, so as to optimize IP capacity decisions.

Methods: The researchers simulated a novel queueing model specifically developed for the IPs. The model takes into account salient features of patient-flow dynamics and was validated against hospital census data. The team used the model to evaluate inpatient capacity decisions against multiple waiting time outcomes: (1) daily average, peak-hour average, and daily maximum waiting times; and (2) proportion of patients waiting strictly more than 0, 1, and 2 hours. The results were published in a simple Microsoft Excel toolbox to allow administrators to conduct sensitivity analysis.

Results: To achieve the hospital's goal of rooming patients within 30 to 60 minutes of IP bed requests, the model predicted that the optimal daily average occupancy levels should be 89%-92% (182-188 beds) in the Medicine cohort, 74%-79% (41-43 beds) in the Cardiology cohort, and 72%-78% (23-25 beds) in the Observation cohort. Larger IP cohorts can achieve the same queueing-related performance measure as smaller ones, while tolerating a higher occupancy level. Moreover, patient waiting time increases rapidly as the occupancy level approaches 100%.

Conclusion: No universal optimal IP occupancy level exists. Capacity decisions should therefore be made on a cohort-by-cohort basis, incorporating the comprehensive patient-flow characteristics of each cohort. To this end, patient-flow queueing models tailored to the IPs are needed.
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http://dx.doi.org/10.1016/j.jcjq.2021.02.008DOI Listing
June 2021

Wealth and the utilization of long-term care services: evidence from the United States.

Int J Health Econ Manag 2021 Mar 30. Epub 2021 Mar 30.

University of Chicago, Chicago, USA.

Long-term care (LTC) provision and financing has become a major challenge for policymakers in the United States and worldwide. To inform associated policies and more efficiently allocate LTC resources, it is important to understand how demand for different types of LTC services responds to increased wealth. We use data from the United States Health and Retirement Study to examine the use of LTC services following plausibly exogenous positive shocks to wealth. We further account for time-invariant household-level characteristics, including the expectation of a wealth shock at an unknown future time, by employing household fixed effects. We find that large positive wealth shocks lead to a greater probability of purchase of paid home care but not of nursing home care. Our results imply that expanding home and community-based services and insurance coverage of home care for people without sufficient wealth is likely to be efficient and welfare improving and should be considered by policymakers.Please confirm if the author names are presented accurately and in the correct sequence (given name, middle name/initial, family name). Author 4 Given name: [R. Tamara] Last name: [Konetzka]. Also, kindly confirm the details in the metadata are correct.confirmedPlease confirm the city are correct and amend if necessary in Affiliations 1, 2, 3, 4.confirmed.
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http://dx.doi.org/10.1007/s10754-021-09299-1DOI Listing
March 2021

Role of organic anion transporter 3 in the renal excretion of biapenem and potential drug-drug interactions.

Eur J Pharm Sci 2021 Jul 19;162:105814. Epub 2021 Mar 19.

Department of Pharmacy, Shanghai Pudong New Area Gongli Hospital, The Second Military Medical University, 219 Miaopu Road, Shanghai 200135, PR China. Electronic address:

Biapenem is a carbapenem antibiotic. It is excreted predominantly through the kidney as unchanged forms. However, the molecular mechanism of renal excretion of biapenem and potential drug-drug interactions (DDIs) were still unknown. In the present study, the role of organic anion transporters (OAT) 1/3 and organic cation transporters (OCT) 2 in the renal excretion of biapenem, and the potential DDIs between biapenem and six clinical commonly prescribed antibiotics and antiviral drugs that acted as substrates or inhibitors of OAT3 were evaluated in vitro. Further, the effect of probenecid on the pharmacokinetics of biapenem was explored in the rats. We observed that biapenem could not inhibit the transport activities of OAT1 or OCT2, while mildly inhibited OAT3 (IC >500 μM). Among the tested antibiotics and antiviral drugs, the relatively high DDI index values (maximal unbound plasma concentration over IC, I/IC) were found for piperacillin, linezolid and benzylpenicillin, which were 2.84, 1.7 and 0.62, respectively. Although probenecid had the highest DDI index (27.1) in vitro, no significant impact of it on the pharmacokinetics of biapenem was observed in the rats. Our results indicated that biapenem was primarily eliminated by the glomerular filtration, while OAT3-mediated renal tubular secretion was a minor route. Biapenem is not a clinically relevant substrate or inhibitor because of its low affinity to OAT3. According to current results, it would be safe to use biapenem with other antibiotics and antiviral drugs that acted as substrates or inhibitors of OAT3.
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http://dx.doi.org/10.1016/j.ejps.2021.105814DOI Listing
July 2021

Complete chloroplast genome sequencing of ten wild Fragaria species in China provides evidence for phylogenetic evolution of Fragaria.

Genomics 2021 May 8;113(3):1170-1179. Epub 2021 Mar 8.

Beijing Academy of Forestry and Pomology Sciences; Beijing Engineering Research Center for Strawberry; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, 100093 Beijing, China. Electronic address:

Complete chloroplast genomes of ten wild Fragaria species native to China were sequenced. Phylogenetic analysis clustered Fragaria species into two clades: The south clade (F. iinumae, F. chinensis, F. pentaphylla, F. nilgerrensis, F. daltoniana, F. corymbosa, F. moupinensis, F. tibetica, F. nipponica, F. gracilis, and F. nubicola and north clade (F. viridis, F. orientalis, F. moschata, F. mandshurica, F. vesca, F. chiloensis, F. virginiana, and F. × ananassa), while F. iinumae is the oldest extant species. Molecular clock analysis suggested present Fragaria species share a common ancestor 3.57 million years ago (Ma), F. moschata and octoploid species evolve 0.89 and 0.97 Ma, respectively, but F. moschata be not directly involved in current octoploid species formation. Drastic global temperature change since the Palaeocene-Eocene, approx. 55 Ma, especially during uplifting of the Qinghai-Tibet plateau and quaternary glaciation may have driven the formation of Fragaria, separation of two groups and polyploidization.
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http://dx.doi.org/10.1016/j.ygeno.2021.01.027DOI Listing
May 2021

Pharmacokinetics, bioavailability, and tissue disposal profiles of Tiamulin fumarate in Nile tilapia (Oreochromis niloticus) following oral and intravenous administrations.

J Vet Pharmacol Ther 2021 Jul 6;44(4):590-602. Epub 2021 Mar 6.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.

Tiamulin fumarate (TIF) is a pleuromutilin antibiotic and has high activity against animal bacterial pathogens including aquatic bacterial pathogens. However, its pharmacokinetic profiles, tissue distribution characteristics and bioavailability in aquatic animals remain unknown. The objective of this study was to investigate the pharmacokinetics and tissue distribution regularities of TIF in tilapia (Oreochromis niloticus) following a single oral (PO) dose of 20 mg/kg body weight (bw) and a single intravenous (IV) dose of 5 mg/kg bw at 22 ± 1°C, respectively. TIF concentrations in tilapia plasma and tissues were determined using the isotope dilution HPLC-HESI-MS/MS procedure, which was validated according to the guidelines defined by US Food and Drug Administration. TIF was well distributed throughout the body compartments of tilapia judged by the apparent volume of distribution (V ) >1 L/kg (6.69 L/kg PO and 1.78 L/kg IV). TIF had a short mean residence time (MRT; 22.82 h PO and 14.61 h IV) and quick total body clearance (CL ) (0.62 L kg  h PO and 0.60 L kg  h IV). The total area under the curve (AUC ) of plasma were 32.25 μg h  ml (PO) and 8.30 μg h  ml (IV), respectively, and the oral absolute bioavailability (F%) of TIF was calculated to be approximately 97.1%. For tissue distribution, high concentrations of TIF were found in kidney, and the longest MRT was recorded in bile. The withdrawal time (WT) of TIF in muscle, skin, liver, kidney, gill, and bile was 3.75 (4) and 1.79 (2), 1.77 (2) and 2.06 (3), 6.41 (7) and 1.97 (2), 6.95 (7) and 3.98 (4), 4.92 (5) and 2.36 (3), and 7.06 (8) and 6.16 (7) days after PO and IV administration, respectively. The present investigations indicated that TIF was quickly absorbed, well distributed, rapidly eliminated in tilapia, and it could serve as reference data for establishing use regimen and provide useful information for the further development of TIF in aquaculture.
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http://dx.doi.org/10.1111/jvp.12957DOI Listing
July 2021
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