Publications by authors named "Jing Deng"

376 Publications

GC-MS-Based Serum Metabolomic Investigations on the Ameliorative Effects of Polysaccharide from in Hyperlipidemia Rats.

Oxid Med Cell Longev 2021 22;2021:9180635. Epub 2021 Jul 22.

Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China.

Hyperlipidemia, a typical metabolic disorder syndrome, can cause various cardiovascular diseases. The polysaccharides were found to have enormous potential in the therapy of hyperlipidemia. This study was aimed at evaluating the ameliorative effects of polysaccharide from (TFP) in rats with hyperlipidemia. A serum metabolomic method based on gas chromatography-mass spectrometry (GC-MS) was used to explore the detailed mechanism of TFP in rats with hyperlipidemia. The oxidative stress indicators, biochemical indexes, and inflammatory factors in serum and histopathological changes in the liver were also evaluated after 10-week oral administration of TFP in rats with high-fat diet-induced hyperlipidemia. TFP significantly relieved oxidative stress, inflammation, and liver histopathology and reduced blood lipid levels. Multivariate statistical approaches such as principal component analysis and orthogonal projection to latent structure square-discriminant analysis revealed clear separations of metabolic profiles among the control, HFD, and HFD+TFP groups, indicating a moderating effect of TFP on the metabolic disorders in rats with hyperlipidemia. Seven metabolites in serum, involved in glycine, serine, and threonine metabolism and aminoacyl-tRNA biosynthesis, were selected as potential biomarkers in rats with hyperlipidemia and regulated by TFP administration. It was concluded that TFP had remarkable potential for treating hyperlipidemia. These findings provided evidence for further understanding of the mechanism of action of TFP on hyperlipidemia.
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http://dx.doi.org/10.1155/2021/9180635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8321759PMC
July 2021

Highly efficient removal of DEET by UV-LED irradiation in the presence of iron-containing coagulant.

Chemosphere 2021 Jul 20;286(Pt 1):131613. Epub 2021 Jul 20.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.

N,N-Diethyl-3-methyl benzoyl amide (DEET) has been detected as an emerging pollutant in various water bodies because of its widespread use as an insect repellent. In this study, the combination of UV-LED and iron-containing coagulant (FeCl) was used for the elimination of DEET in water. It was found that UV-LED/FeCl (98 %) system presented a favorable removal of DEET compared with UV/FeCl (59 %) and UV-LED/Fe(SO) (81 %) processes at initial pH 3.5. DEET degradation by both UV-LED/FeCl and UV-LED/Fe(SO) processes followed pseudo-first-order kinetics with the calculated pseudo-first-order rate constants (k) of 0.0105 and 0.0046 cm mJ, respectively. The results of ESR analysis and radicals quenching experiments indicated that hydroxyl radicals (OH) and superoxide radicals (O) were responsible for DEET degradation in UV-LED/FeCl process, and the former played the major role. An increase in FeCl dosage was beneficial to the degradation. In the UV-LED/FeCl process, DEET degradation increased with a decrease in pH from 3.5 to 3.0, whereas it was almost completely suppressed with an increase in pH from 4.3 to 6.3. DEET degradation was almost unchanged after the introduction of NO, and it impeded after the addition of humic acid (HA), HCO, and SO. The plausible degradation pathway mainly involved hydroxylation, cleavage of the C-N bond, acetylation, and dealkylation. Among the disinfection by-products (DBPs) evaluated, UV-LED/FeCl pretreatment generally increased the generation of trichloromethane, chloral hydrate, dichloroacetic acid, and trichloroacetic acid, which implied that further assessment of environmental risk was needed during its practical applications.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131613DOI Listing
July 2021

The power and the promise of circRNAs for cancer precision medicine with functional diagnostics and prognostic prediction.

Carcinogenesis 2021 Jul 27. Epub 2021 Jul 27.

National Engineering Laboratory for Deep Process of Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China.

Circular RNA (circRNA) is a large class of covalently closed circular RNA. As a member of competitive endogenous RNA (ceRNA), it participates in the regulation of circRNA-miRNA-mRNA network and plays an important role in the regulation of physiology and pathology. CircRNA is produced by the reverse splicing of exon, intron or both, forming exon or intron circRNA. Studies have shown that circRNA is a ubiquitous molecule, which exceeds the linear mRNA distributed in human cells. Because of its covalent closed-loop structure, circRNA is resistant to RNase R, which is more stable than linear mRNA; circRNA is highly conserved in different species. It was found that circRNA competitively adsorbs miRNA, as a miRNA sponge, to involve in the expression regulation of a variety of genes and plays an important role in tumor development, invasion, metastasis and other processes. These molecules offer new potential opportunities for therapeutic intervention and serve as biomarkers for diagnosis. In this paper, the origin, characteristics and functions of circRNA and its role in tumor development, invasion and metastasis, diagnosis and prognosis are reviewed.
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http://dx.doi.org/10.1093/carcin/bgab071DOI Listing
July 2021

Targeted silencing of TEM8 suppresses non‑small cell lung cancer tumor growth via the ERK/Bcl‑2 signaling pathway.

Mol Med Rep 2021 Aug 24;24(2). Epub 2021 Jun 24.

Department of Palliative Medicine, Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China.

Non‑small cell lung cancer (NSCLC) is one of the most common malignancies with high rates of mortality. Although great progress has been made with the development of novel immunotherapies and targeted therapeutic strategies, the 5‑year total survival rate of lung cancer has remained unchanged over the past few decades. Therefore, more effective therapeutics are urgently needed. Tumor endothelial marker 8 (TEM8) is an integrin‑like cell surface transmembrane protein that has been demonstrated to be upregulated in numerous cancer types and previously showed promise for targeted cancer therapy. However, the role of TEM8 in NSCLC remains poorly understood. The present study aimed to investigate the effects of silencing TEM8 on expression and regulation of extracellular signal‑regulated kinase (ERK)1/2 signaling pathways in NSCLC. In the present study, a lentiviral vector that encoded a short hairpin RNA targeting TEM8 was designed and transfected into Xuanwei Lung Cancer (XWLC)‑05 lung cancer cells to silence TEM8 expression. Male BALB/c‑nu/nu mice were then given subcutaneous injections in the right dorsal flank with XWLC‑05 cells. Microvessel density was measured using an anti‑CD34 antibody. The mRNA and protein levels of ERK1/2 and Bcl‑2 in XWLC‑05 cells or xenograft tumor tissues were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting. TEM8 knockdown was found to significantly inhibit tumor growth and conferred an anti‑angiogenic ability . Furthermore, TEM8 knockdown suppressed the expression of Bcl‑2 mediated by ERK1/2 activity in XWLC‑05 cells or tissues from mice with NSCLC. To conclude, these results suggest that the targeted silencing of TEM8 may serve as an effective method of treating NSCLC.
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http://dx.doi.org/10.3892/mmr.2021.12234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240451PMC
August 2021

A Qualitative Investigation of the Psychological Experiences of COVID-19 Patients Receiving Inpatient Care in Isolation.

Clin Nurs Res 2021 Jun 19:10547738211024807. Epub 2021 Jun 19.

Department of Anesthesiology, Army Medical Center of PLA, Chongqing, China.

Coronavirus disease 2019 (COVID-19) has spread rapidly throughout the world. Still, little is known about the psychological experiences of patients who received inpatient isolation treatment in order to improve the well-being of these patients. We randomly recruited 10 COVID-19 patients who received inpatient isolation treatment at a designated hospital in Wuhan from February to March 2020 and were discharged after recovery. The data were collected via a semi-structured interview over WeChat video and analyzed them using Calaizzi's descriptive phenomenological method. COVID-19 patients experienced significant psychological stress during hospitalization that continued after recovery and discharge. This can be categorized into three themes: (1) negative emotions experienced; (2) uncertainty of treatment provided; and (3) worries about readjusting to daily life. The insight into a patient's psychological experiences can support the timely implementation of personalized nursing interventions within hospitals and the community to improve the patient's mental well-being and recovery trajectory.
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http://dx.doi.org/10.1177/10547738211024807DOI Listing
June 2021

Toward improvements for enhancement the productivity and color value of pigments: a critical with recent updates.

Crit Rev Food Sci Nutr 2021 Jun 16:1-15. Epub 2021 Jun 16.

National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Province Key Laboratory of Edible forestry Resources Safety and Processing Utilization, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, China.

pigments are a kind of high-quality natural edible pigments fermented by filamentous fungi, which have been widely used in food, cosmetics, medicine, textiles, dyes and chemical industries as active functional ingredients. Moreover, pigments have a good application prospect because of a variety of biological functions such as antibacterial, antioxidation, anti-inflammatory, regulating cholesterol, and anti-cancer. However, the low productivity and color value of pigments restrict their development and application. In this review, we introduced the categories, structures, biosynthesis and functions of pigments, and summarized the current methods for improving the productivity and color value of pigments, including screening and mutagenesis of strains, optimization of fermentation conditions, immobilized fermentation, mixed fermentation, additives, gene knockout and overexpression technologies, which will help to develop the foundation for the industrial production of pigments.
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http://dx.doi.org/10.1080/10408398.2021.1935443DOI Listing
June 2021

Precision Breast-Conserving Surgery With Microwave Ablation Guidance: A Pilot Single-Center, Prospective Cohort Study.

Front Oncol 2021 26;11:680091. Epub 2021 May 26.

Department of Breast Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.

Introduction: Negative margins in breast-conserving surgery (BCS) are essential for preventing recurrence. The aim of this study was to determine the use of preoperative microwave ablation (MWA) in the guidance of BCS for early-stage breast cancer and access whether MWA could influence the rates of positive resection margins.

Methods: From 2016 to 2018, 22 women with T1/T2 invasive breast cancer were enrolled for MWA prospectively in the guidance of BCS. US-guided MWA was performed under local anesthesia, followed by BCS and sentinel lymph node biopsy (SLNB) one week after ablation. Women who underwent palpation-guided BCS directly were included as control, and propensity score matching analysis was applied.

Results: MWA was performed in 22 patients. Of the 21 MWA cases with effect information, the mean tumor size in US was 20.9 ± 6.2 mm (6-37 mm). Compared with control group (BCS directly), a lower rate of positive/close margins was observed in MWA guidance group ( = 0.018), and MWA caused a higher rate of accurate surgery (the largest margin ≤ 3 cm and the smallest margin ≥ 1mm, = 0.042). Of these 21 patients treated with MWA, 18 were candidates for SLNB. And sentinel lymph nodes were successfully identified in all cases, and no recurrence was found with a mean follow-up of 23 months.

Conclusion: For patients with T1/T2 breast cancer, the application of preoperative MWA could guide BCS accurately without impairing SLNB. Clinical trials with long-term results are required to validate MWA in the guidance for breast cancer excision.
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http://dx.doi.org/10.3389/fonc.2021.680091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187871PMC
May 2021

[Study of salivary metabonomics in patients with oral squamous cell carcinoma and leukoplakia].

Shanghai Kou Qiang Yi Xue 2021 Apr;30(2):201-205

Department of Stomatology, Affiliated Peace Hospital of Changzhi Medical College. Changzhi 046000,Shanxi Province, China.

Purpose: The objective of this study was to detect and analyze the existence of differential metabolites from saliva of patients with oral squamous cell carcinoma(OSCC) by metabonomics method, and to evaluate its application value on clinical diagnosis or screening of OSCC.

Methods: The collected saliva samples for OSCC patients, oral leukoplakiac(OLK) patients who were pathologically diagnosed and met the inclusion criteria were analyzed using metabonomics methods (GC-TOF-MS). The results were analyzed by principal component analysis and orthogonal partial least squares discriminant analysis using SIMCA software.

Results: The metabolic profiles of OSCC, OLK and healthy control group showed significant differences (P<0.05). In total, 15 typical differential metabolites among the three groups were detected. Further study focusing on metabolic pathway revealed imbalance of protein, energy and lipid metabolism between OSCC and OLK patients. Abnormal tricarboxylic acid cycle was detected as well.

Conclusions: Metabonomics methods is feasible for differential metabolites analysis,15 differential metabolites were detected in OSCC,OLK patients and healthy people. These findings will contribute to the early screening of oral squamous cell carcinoma.
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April 2021

Quantitative proteomics characterization of cancer biomarkers and treatment.

Mol Ther Oncolytics 2021 Jun 20;21:255-263. Epub 2021 Apr 20.

National Engineering Laboratory for Deep Process of Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China.

Cancer accounted for 16% of all death worldwide in 2018. Significant progress has been made in understanding tumor occurrence, progression, diagnosis, treatment, and prognosis at the molecular level. However, genomics changes cannot truly reflect the state of protein activity in the body due to the poor correlation between genes and proteins. Quantitative proteomics, capable of quantifying the relatively different protein abundance in cancer patients, has been increasingly adopted in cancer research. Quantitative proteomics has great application potentials, including cancer diagnosis, personalized therapeutic drug selection, real-time therapeutic effects and toxicity evaluation, prognosis and drug resistance evaluation, and new therapeutic target discovery. In this review, the development, testing samples, and detection methods of quantitative proteomics are introduced. The biomarkers identified by quantitative proteomics for clinical diagnosis, prognosis, and drug resistance are reviewed. The challenges and prospects of quantitative proteomics for personalized medicine are also discussed.
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http://dx.doi.org/10.1016/j.omto.2021.04.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142045PMC
June 2021

Exoskeleton Active Walking Assistance Control Framework Based on Frequency Adaptive Dynamics Movement Primitives.

Front Neurorobot 2021 20;15:672582. Epub 2021 May 20.

Robotics Institute, Shenzhen Academy of Aerospace Technology, Shenzhen, China.

This paper introduces a novel exoskeleton active walking assistance control framework based on frequency adaptive dynamics movement primitives (FADMPs). The FADMPs proposed in this paper is an online learning and prediction algorithm which is able to online estimate the fundamental frequency of human joint trajectory, learn the shape of joint trajectory and predict the future joint trajectory during walking. The proposed active walking assistance control framework based on FADMPs is a model-based controller which relies on the human joint torque estimation. The assistance torque provided by exoskeleton is estimated by human lower limb inverse dynamics model which is sensitive to the noise in the joint motion trajectory. To estimate a smooth joint torque profile, the joint motion trajectory must be filtered first by a lowpass filter. However, lowpass filter will introduce an inevitable phase delay in the filtered trajectory. Both simulations and experiments in this paper show that the phase delay has a significant effect on the performance of exoskeleton active assistance. The active assistant control framework based on FADMPs aims at improving the performance of active assistance control by compensating the phase delay. Both simulations and experiments on active walking assistance control show that the performance of active assistance control can be further improved when the phase delay in the filtered trajectory is compensated by FADMPs.
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http://dx.doi.org/10.3389/fnbot.2021.672582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173117PMC
May 2021

The therapeutic effects of Prunella vulgaris against fluoride-induced oxidative damage by using the metabolomics method.

Environ Toxicol 2021 Jun 5. Epub 2021 Jun 5.

Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha, China.

Fluoride is considered as one of the most ubiquitous environmental pollutants. Numerous studies have linked reactive oxygen species (ROS)-dependent oxidative damage with fluoride intoxication, which could be prevented by antioxidants. However, the metabolomic changes induced by ROS disruptions in fluoride intoxication are yet unknown. The present study aimed to provide novel mechanistic insights into the fluoride-induced oxidative damage and to investigate the potential protective effects of ethanolic extract of Prunella vulgaris (natural antioxidant, PV) against fluoride-induced oxidative damage. The serum biochemical indicators related to fluoride-induced oxidative damage, such as lipid peroxidation parameter, inflammation and marker enzymes in the liver increased significantly in the fluoride-treated group, while antioxidant enzymes were decreased. However, PV treatment restored the level of these biochemical indicators, indicating satisfactory antioxidant, anti-inflammatory, and hepatoprotective potential of PV. The metabolomics analysis in the serum was performed by liquid chromatography-mass spectroscopy, whereas the fluoride treatment caused severe metabolic disorders in rats, which could be improved by PV. The differential metabolites screened by multivariate analysis after fluoride and PV treatment, were organic acids, fatty acids, and lipids. These differential metabolites represented disorders of glyoxylate and dicarboxylate metabolism and the citrate cycle (TCA) according to metabolic pathway analysis in fluoride treatment rats. Interestingly, the result of metabolic pathway analysis of post-treatment with PV was consistent with that of fluoride treatment, indicating that the energy metabolism plays a major role in the progress of fluoride-induced oxidative damage, as well as the therapeutic effect of PV. These findings provided a theoretical basis for understanding the mechanism underlying metabolic disorders of fluoride toxicity and the effect of PV.
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http://dx.doi.org/10.1002/tox.23301DOI Listing
June 2021

Unexpected Role of Achiral Glycine in Determining the Suprastructural Handedness of Peptide Nanofibrils.

ACS Nano 2021 06 28;15(6):10328-10341. Epub 2021 May 28.

State Key Laboratory of Heavy Oil Processing and School of Materials Science and Engineering, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China.

Helical supramolecular architectures play important structural and functional roles in biological systems. Although their occurrence is widely perceived to correlate to fundamental chiral units including l-amino acids and d-sugars, the detailed relationship between molecular and supramolecular handedness is still unclear. At the same time, although achiral units are practically always in close proximity to chiral ones by covalent linkage along a polymeric chain, their effect on supramolecular handedness has received relatively less attention. Here, we designed a set of short amphiphilic peptides, in which an achiral glycine residue was incorporated at the interface between the hydrophobic and hydrophilic segments. We observed that glycine incorporation caused dramatic variations in suprastructural handedness in self-assembled peptide nanofibrils, and the effect of the hydrophilic charged residue at the C-terminus on supramolecular handedness was demolished, leading to chiral truncation. Furthermore, molecular dynamics simulations and quantum chemistry calculations revealed that the unanticipated role of the glycine residue in regulating supramolecular handedness originated from its effect on the conformational preference of single β-strands. Importantly, reduced density gradient analyses on single β-strands indicated that, due to the lack of a side chain in glycine, intricate noncovalent interactions were produced among the neighboring amino acid side chains of the incorporated glycine and its local backbone, resulting in diverse β-strand conformations.
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http://dx.doi.org/10.1021/acsnano.1c02547DOI Listing
June 2021

Successful prenatal therapy of anti-CD36-mediated severe FNAIT by deglycosylated antibodies in a novel murine model.

Blood 2021 05 26. Epub 2021 May 26.

Institute of Blood Transfusion, Guangzhou Blood Center, China, Guangzhou, China.

Recent studies have demonstrated that maternal anti-CD36 antibodies represent a frequent cause of fetal/neonatal alloimmune thrombocytopenia (FNAIT) in Asian and African populations. However, little is known about the pathomechanism and antenatal treatment of anti-CD36-mediated FNAIT. Here, we established a novel animal model to examine the clinical features of pups from immunized Cd36-/- female mice after breeding with wild-type male mice. Mild thrombocytopenia was observed, but high pup mortality was also documented (40.26%). IVIG (1 g/kg) administration on days 7, 12, and 17 to immunized Cd36-/- mothers after breeding reduced fetal death (12.70%). However, delaying the IVIG administration series on days 10, 15, and 20 did not reduce fetal death (40.00%). In contrast, injection of deglycosylated anti-CD36 (deg-anti-CD36) polyclonal antibodies (5 mg/kg) on days 10, 15, and 20 significantly reduced fetal death (5.26%). Subsequently, monoclonal antibodies (mAbs) against mouse CD36 were developed, and one clone producing high-affinity anti-CD36 (termed 32-106) effectively inhibited maternal antibody binding and was therefore selected. Using the same approach of deg-anti-CD36, the administration of deg-32-106 significantly reduced fetal death (2.17%). Furthermore, immunized Cd36-/- mothers showed placenta deficiency. Accordingly, maternal anti-CD36 antibodies inhibited angiogenesis of placenta endothelial cells, which could be restored by deg-32-106. In summary, maternal anti-CD36 antibodies caused a high frequency of fetal death in our animal model, associated with placental dysfunction. This deleterious effect could be diminished by the antenatal administration of IVIG and deg-mAb 32-106. Interestingly, treatment with deg-32-106 appears more beneficial considering the lower dose, later start of treatment, and therapy success.
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http://dx.doi.org/10.1182/blood.2021011131DOI Listing
May 2021

The R2R3-MYB transcription factor GhMYB1a regulates flavonol and anthocyanin accumulation in Gerbera hybrida.

Hortic Res 2020 May 20;7(1):78. Epub 2020 May 20.

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.

Anthocyanins and flavonols have vital roles in flower coloration, plant development, and defense. Because anthocyanins and flavonols share the same subcellular localization and common biosynthetic substrates, these pathways may compete for substrates. However, the mechanism regulating this potential competition remains unclear. Here, we identified GhMYB1a, an R2R3-MYB transcription factor involved in the regulation of anthocyanin and flavonol accumulation in gerbera (Gerbera hybrida). GhMYB1a shares high sequence similarity with that of other characterized regulators of flavonol biosynthesis. In addition, GhMYB1a is also phylogenetically grouped with these proteins. The overexpression of GhMYB1a in gerbera and tobacco (Nicotiana tabacum) resulted in decreased anthocyanin accumulation and increased accumulation of flavonols by upregulating the structural genes involved in flavonol biosynthesis. We further found that GhMYB1a functions as a homodimer instead of interacting with basic helix-loop-helix cofactors. These results suggest that GhMYB1a is involved in regulating the anthocyanin and flavonol metabolic pathways through precise regulation of gene expression. The functional characterization of GhMYB1a provides insight into the biosynthesis and regulation of flavonols and anthocyanins.
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http://dx.doi.org/10.1038/s41438-020-0296-2DOI Listing
May 2020

Discovery of first-in-class inhibitors of ASH1L histone methyltransferase with anti-leukemic activity.

Nat Commun 2021 05 14;12(1):2792. Epub 2021 May 14.

Department of Pathology, University of Michigan, Ann Arbor, MI, USA.

ASH1L histone methyltransferase plays a crucial role in the pathogenesis of different diseases, including acute leukemia. While ASH1L represents an attractive drug target, developing ASH1L inhibitors is challenging, as the catalytic SET domain adapts an inactive conformation with autoinhibitory loop blocking the access to the active site. Here, by applying fragment-based screening followed by medicinal chemistry and a structure-based design, we developed first-in-class small molecule inhibitors of the ASH1L SET domain. The crystal structures of ASH1L-inhibitor complexes reveal compound binding to the autoinhibitory loop region in the SET domain. When tested in MLL leukemia models, our lead compound, AS-99, blocks cell proliferation, induces apoptosis and differentiation, downregulates MLL fusion target genes, and reduces the leukemia burden in vivo. This work validates the ASH1L SET domain as a druggable target and provides a chemical probe to further study the biological functions of ASH1L as well as to develop therapeutic agents.
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http://dx.doi.org/10.1038/s41467-021-23152-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121805PMC
May 2021

[Metabolic mechanism of Prunella vulgaris in treatment of ethanol-induced oxidative stress in rats based on metabonomics].

Zhongguo Zhong Yao Za Zhi 2021 Apr;46(7):1813-1821

Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine Changsha 410208, China.

Prunella vulgaris(PV) is an edible and traditional medicinal herb which has a wide range application in fighting inflammation and oxidative stress, and protecting liver. Now it has been used to treat various types of liver diseases and has significant clinical efficacy. This study aims to investigate the effects of PV on ethanol-induced oxidative stress injury in rats and its metabolic mechanism. The rats were divided into control group, model group, PV group, and VC group. The liver protection of PV was identified by measuring pharmacological indexes such as antioxidant and anti-inflammatory activity. The metabolic mechanism of long-term ethanol exposure and the metabolic regulation mechanism of PV treatment were studied by LS-MS metabonomics. The pharmacological investigation indicated that ethanol could significantly decrease the contents of SOD, GSH-Px, CAT and other antioxidant enzymes in liver and increase the content of MDA. At the same time, PV could significantly reduce the contents of inflammatory factors(TNF-α, IL-6 and IL-1β) and liver function markers(ALT, AST, ALP) in serum. What's more, long-term ethanol exposure could significantly cause liver injury, while PV could protect liver. Metabolomics based on multiple statistical analyses showed that long-term ethanol exposure could cause significant metabolic disorder, and fatty acids, phospholipids, carnitines and sterols were the main biomarkers. Meanwhile, pathway analysis and enrichment analysis showed that the β oxidation of branched fatty acids was the main influencing pathway. Also, PV could improve metabolic disorder of liver injury induced by ethanol, and amino acids, fatty acids, and phospholi-pids were the main biomarkers in PV treatment. Metabolic pathway analysis showed that PV mainly regulated metabolic disorder of ethanol-induced liver injury through phenylalanine, tyrosine and tryptophan biosynthetic pathways. This study could provide a new perspective on the hepatoprotective effect of natural medicines, such as PV.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210122.503DOI Listing
April 2021

Corrigendum: Effect of a Humanized Diet Profile on Colonization Efficiency and Gut Microbial Diversity in Human Flora-Associated Mice.

Front Nutr 2021 26;8:676085. Epub 2021 Apr 26.

College of Food Science, South China Agricultural University, Guangzhou, China.

[This corrects the article DOI: 10.3389/fnut.2021.633738.].
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http://dx.doi.org/10.3389/fnut.2021.676085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107682PMC
April 2021

Effect of lipopolysaccharide on calcification of human umbilical artery smooth muscle cells co-cultured with human periodontal ligament cells.

Exp Ther Med 2021 Jun 20;21(6):655. Epub 2021 Apr 20.

Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.

Periodontitis is an independent risk factor for coronary heart disease. lipopolysaccharide (Pg-LPS) was considered to be one of the main virulence factors. In addition, vascular smooth muscle cells transform into osteoblast-like cells in an arterial calcification process under chronic inflammatory conditions. The present study aimed to determine the calcification induced by Pg-LPS in human umbilical artery smooth muscle cells (HUASMCs) co-cultured with human periodontal ligament cells (HPDLCs). An co-culture system was established using Transwell inserts. HUASMC proliferation and alkaline phosphatase (ALP) activity were measured with a Cell Counting Kit-8 and an ALP kit, respectively. Calcium nodule formation was detected using alizarin red S staining. The effects of Pg-LPS on the mRNA expression of the calcification genes of ALP, core-binding factor α1 (Runx2) and bone sialoprotein (BSP) were assessed using reverse transcription-quantitative PCR. The results indicated that Pg-LPS increased HUASMC proliferation and ALP activity. Furthermore, among all of the groups, calcium nodule formation was most extensive in co-cultured cells in the mineralization-inducing medium containing Pg-LPS. In addition, the expression of specific osteogenic genes (Runx2, ALP and BSP) significantly increased in the presence of Pg-LPS and mineralization-inducing medium, which was further enhanced in co-culture with HPDLCs. In conclusion, co-culture with HPDLCs increased the effect of Pg-LPS to stimulate the calcification of HUASMCs. It was suggested that besides the inflammation, periodontitis may promote the occurrence of vascular calcification. The study indicated that periodontal treatment of subgingival scaling to reduce and/or control may decrease the occurrence or severity of vascular calcification.
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http://dx.doi.org/10.3892/etm.2021.10087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097230PMC
June 2021

MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models.

Cell Death Discov 2021 May 3;7(1):90. Epub 2021 May 3.

Ascentage Pharma (Suzhou) Co., Ltd., 218 Xinghu Street, Suzhou, Jiangsu Province, China.

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous clonal disease associated with unmet medical needs. Paralleling the pathology of other cancers, AML tumorigenesis and propagation can be ascribed to dysregulated cellular processes, including apoptosis. This function and others are regulated by tumor suppressor P53, which plays a pivotal role in leukemogenesis. Opposing P53-mediated activities is the mouse double minute 2 homolog (MDM2), which promotes P53 degradation. Because the TP53 mutation rate is low, and MDM2 frequently overexpressed, in patients with leukemia, targeting the MDM2-P53 axis to restore P53 function has emerged as an attractive AML treatment strategy. APG-115 is a potent MDM2 inhibitor under clinical development for patients with solid tumors. In cellular cultures and animal models of AML, we demonstrate that APG-115 exerted substantial antileukemic activity, as either a single agent or when combined with standard-of-care (SOC) hypomethylating agents azacitidine (AZA) and decitabine (DAC), or the DNA-damaging agent cytarabine (Ara-C). By activating the P53/P21 pathway, APG-115 exhibited potent antiproliferative and apoptogenic activities, and induced cell cycle arrest, in TP53 wild-type AML lines. In vivo, APG-115 significantly reduced tumor burden and prolonged survival. Combinations of APG-115 with SOC treatments elicited synergistic antileukemic activity. To explain these effects, we propose that APG-115 and SOC agents augment AML cell killing by complementarily activating the P53/P21 pathway and upregulating DNA damage. These findings and the emerging mechanism of action afford a sound scientific rationale to evaluate APG-115 (with or without SOC therapies) in patients with AML.
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http://dx.doi.org/10.1038/s41420-021-00465-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093284PMC
May 2021

ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway.

Acta Biochim Pol 2021 Apr;68(2):223-229

Department of Digestive Tumor Surgery, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, 210029, Chin.

ADP-ribosylation factor 3 (ARF3) has confirmed participate in diverse biological processes in many cancers. However, the expression patterns and roles of ARF3 in gastric cancer (GC) remains largely unknown. In our study, by using qRT-PCR and western blot, we found that, in In GC tissues and cells, the expression of ARF3 was significantly down-regulated. Functional experiments demonstrated that ARF3 inhibited proliferation, induced cycle arrest and enhanced apoptosis of GC cells. Moreover, by performing western blot, we found that ARF3 could regulate the protein expression of key factors of AKT and ERK pathway. Using orthotopic xenograft mouse models, it is showed that ARF3 could inhibit GC tumorigenesis in vivo. To sum up, ARF3 may suppress proliferation, induced cycle arrest and promotes apoptosis of GC by modulating AKT and ERK pathway. It might act as a potential biomarker for GC prognosis.
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http://dx.doi.org/10.18388/abp.2020_5519DOI Listing
April 2021

Enhanced Eradication of Bacterial/Fungi Biofilms by Glucose Oxidase-Modified Magnetic Nanoparticles as a Potential Treatment for Persistent Endodontic Infections.

ACS Appl Mater Interfaces 2021 Apr 7;13(15):17289-17299. Epub 2021 Apr 7.

Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.

Bacterial/fungal biofilm-mediated persistent endodontic infections (PEIs) are one of the most frequent clinical lesions in the oral cavity, resulting in apical periodontitis and tooth damage caused by loss of minerals. The conventional root canal disinfectants are poorly bio-safe and harmful to teeth and tissues, making them ineffective in treating PEIs. The development of nanomaterials is emerging as a promising strategy to eradicate disease-related bacteria/fungi. Herein, glucose oxidase (GOx)-modified magnetic nanoparticles (MNPs) were synthesized a facile and versatile route for investigating their effects on removing PEI-related bacterial/fungal biofilms. It is found that GOx was successfully immobilized on the MNPs by detecting the changes in the diameter, chemical functional group, charge, and magnetic response. Further, we demonstrate that GOx-modified MNPs (GMNPs) exhibit highly effective antibacterial activity against and . Moreover, the antibacterial/fungal activity of GMNPs is greatly dependent on their concentrations. Importantly, when placed in contact with bacterial/fungal biofilms, the dense biofilm matrix is destructed due to the movement of GMNPs induced by the magnetic field, the formation of reactive oxygen species, and nutrient starvation induced by GOx. Also, the experiment shows that the as-prepared GMNPs have excellent cytocompatibility and blood compatibility. Thus, GMNPs offer a novel strategy to treat bacteria/fungi-associated PEIs for potential clinical applications.
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http://dx.doi.org/10.1021/acsami.1c01748DOI Listing
April 2021

Ascorbic acid enhanced ciprofloxacin degradation with nanoscale zero-valent copper activated molecular oxygen.

Chemosphere 2021 Sep 27;278:130354. Epub 2021 Mar 27.

School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.

The remediation of water polluted by fluroquinolones antibiotics remains an important issue. Although zero-valent copper (ZVC) coupled with molecular oxygen can destruct refractory organic pollutants, the activation efficiency still needs to be further improved. In this study, the introduction of ascorbic acid (AA) in ZVC/air process maintained a high-concentration of Cu(Ⅰ), which can efficiently activate molecular oxygen to generate reactive oxygen species (ROSs). Superoxide radicals and hydroxyl radicals coexisted in nZVC/AA/air system. The former contributed to the yield of HO and also acted as a mediator for Cu(Ⅱ)/Cu(Ⅰ) redox cycles, the latter was the pivotal ROSs for ciprofloxacin (CIP) destruction. The CIP degradation decelerated through the addition of excessive nZVC and AA, and the optimum dosages of nZVC and AA were determined to be 0.2 g/L and 1 mM, respectively. The developed nZVC/AA/air process could efficiently operate in a relative broad pH range of 3.0-7.0, which was due to the fact that AA prevented the precipitation of copper ions in solution via forming stable chelates. The coexistence of Cl severely retarded the CIP removal. According to the results of UPLC-MS/MS analysis and density functional theory calculations, the plausible degradation pathways including the decarboxylation, defluorination, hydroxylation and cleavage of C-C bond in piperazine ring were proposed.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130354DOI Listing
September 2021

Fucoidan as a marine-origin prebiotic modulates the growth and antibacterial ability of Lactobacillus rhamnosus.

Int J Biol Macromol 2021 Jun 20;180:599-607. Epub 2021 Mar 20.

Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China; School of Stomatology, Qingdao University, Qingdao 266003, China. Electronic address:

Fucoidan has received much attention in healthy food and biomedicine owing to their unique (bio)physicochemical properties, particularly antibacterial and antiviral. Pathogenic microorganisms and probiotics are coexisting in many tissues (e.g., gut, oral, and vagina). However, the effect of fucoidan on probiotics has not been examined. Herein, fucoidan sterilized by different methods (i.e., 0.22 μm filter and high-temperature autoclave) is applied to explore its effect on the responses of Lactobacillus rhamnosus. It is found that high-temperature autoclave treatment causes the depolymerization of fucoidan. Further, the proliferation, morphology, and metabolism of probiotics are greatly dependent on the concentrations of fucoidan. The formation of probiotic biofilm is reduced with an increased concentration of fucoidan. Moreover, the antibacterial ability of probiotics initially increases and then decreases with an increased concentration of fucoidan. Thus, fucoidan could serve as a new marine-origin prebiotic, offering new insight into probiotic modulation and its application in inhibiting bacterial infections.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.065DOI Listing
June 2021

Impact of MASP2 gene polymorphism and gene-tea drinking interaction on susceptibility to tuberculosis.

Sci Rep 2021 Mar 22;11(1):6544. Epub 2021 Mar 22.

Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, 110 Xiangya Road, Changsha, 410000, China.

Mannan-binding lectin-associated serine protease-2 (MASP-2) has been reported to play an important role as a key enzyme in the lectin pathway of the complement system. The objectives of our study were to determine whether the single-nucleotide polymorphism (SNPs) of MASP2 and the gene-tea drinking interaction were associated with the susceptibility to TB. In total, 503 patients and 494 healthy controls were contained. Three SNPs (rs12142107, rs12711521, and rs7548659) were genotyped. The association between the SNPs and susceptibility to TB were investigated by conducting multivariate unconditional logistic regression analysis. The gene-tea drinking interactions were analyzed by the additive model of marginal structural linear odds models. Both genotype AC + AA at rs12711521 of MASP2 genes and genotype GT + GG at rs7548659 of MASP2 genes were more prevalent in the TB patient group than the healthy control group (OR: 1.423 and 1.439, respectively, P < 0.05). In addition, The relative excess risk of interaction (RERI) between tea drinking and rs12142107, rs12711521, and rs7548659 of MASP2 genes was found to suggest negative interactions, which reached - 0.2311 (95% confidence interval (CI): - 0.4736, - 0.0113), - 0.7080 (95% CI - 1.3998, - 0.0163), and - 0.5140 (95% CI - 0.8988, - 0.1291), respectively (P < 0.05). Our finding indicated that the SNPs (rs12711521 and rs7548659) of MASP2 were associated with the susceptibility to TB. Furthermore, there were negative interactions between tea drinking and rs12142107, rs12711521, and rs75548659 of MASP2 gene, respectively. Our research provides a basis for studying the pathogenesis and prevention of tuberculosis.
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http://dx.doi.org/10.1038/s41598-021-86129-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985323PMC
March 2021

Upregulation of PEDF Predicts a Poor Prognosis and Promotes Esophageal Squamous Cell Carcinoma Progression by Modulating the MAPK/ERK Signaling Pathway.

Front Oncol 2021 26;11:625612. Epub 2021 Feb 26.

Department of Laboratory Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.

Invasion and metastasis represent the primary causes of therapeutic failure in patients diagnosed with esophageal squamous cell carcinoma (ESCC). The lack of effective treatment strategies for metastatic ESCC is the major cause of the low survival rate. Therefore, it is crucial to understand the molecular mechanisms underlying ESCC metastasis and identify potential biomarkers for targeted therapy. Herein, we reported that PEDF is significantly correlated with tumor cell invasion and metastasis in ESCC. The high expression of PEDF is an independent unfavorable prognostic factor for ESCC patients' overall survival (OS). We successfully developed and verified a nomogram to predict the preoperative OS of ESCC patients, and the actual and nomogram-predicted 1-, 3-, and 5-year survival rates had good consistency. The receiver operating characteristic (ROC) curve showed that the area under the curve (AUC) values for 1-, 3- and 5- survival were 0.764, 0.871, and 0.91, respectively. Overexpression of PEDF significantly promoted the migration and invasion of ESCC cells , while silencing PEDF yielded the opposite effects. Elevated levels of PEDF altered the expression of proteins involved in epithelial-mesenchymal transition (EMT), as indicated by the upregulation of N-cadherin and the downregulation of -catenin and E-cadherin in ESCC cells. Mechanistically, PEDF promoted tumor cell motility and EMT by activating the MAPK/ERK signaling pathway. In conclusion, our results reveal that PEDF is involved in ESCC metastasis and could act as a prognostic factor for ESCC. Our research provides a fresh perspective into the mechanism of ESCC metastasis.
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http://dx.doi.org/10.3389/fonc.2021.625612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953146PMC
February 2021

Effect of a Humanized Diet Profile on Colonization Efficiency and Gut Microbial Diversity in Human Flora-Associated Mice.

Front Nutr 2021 23;8:633738. Epub 2021 Feb 23.

College of Food Science, South China Agricultural University, Guangzhou, China.

Human flora-associated (HFA) mouse models allow us to design interventions for human disease research to test specific hypotheses and explore the complex commensal microbiome while avoiding the ethical limitations of using humans as models to directly study intestinal flora diseases. However, few studies have investigated the effect of a humanized diet profile (coarse-feed diet; CFD) on colonization efficiency and gut microbial diversity in HFA mice. We tested the colonization efficiency and gut microbial diversity in germ-free Kunming (KM) mice fed a CFD or a purified feed diet (PFD) at 1, 2, and 4 weeks. Although the colonization efficiencies differed significantly (67.50-70.00% vs. 72.69-85.96%) in the HFA mice, the colonization efficiency of the PFD-fed HFA mice (85.96%) was significantly higher than that of the CFD-fed mice (69.61%) at 2 weeks. At 4 weeks, the colonization efficiency of the PFD-fed mice (72.69%) was comparable to that of the CFD-fed mice (70.00%). Additionally, the gut microbial diversity of the CFD-fed HFA mice was similar to that of a human fecal donor. Regarding the Kyoto Encyclopedia of Genes and Genomes colonic microbiota metabolic pathways, the CFD-fed HFA mice showed more similarities to the human donor than to the PFD-fed mice in amino sugar and nucleotide sugar metabolism, biosynthesis of amino acids, carbon metabolism, purine metabolism, and phosphotransferase systems. In conclusion, the humanized diet profiles of the CFD and PFD could help establish human microbiotas in mice. Constructing HFA mouse models fed a CFD for 4 weeks may be useful in researching human-derived intestinal diseases.
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http://dx.doi.org/10.3389/fnut.2021.633738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940529PMC
February 2021

Mechanistic insights into paracetamol transformation in UV/NHCl process: Experimental and theoretical study.

Water Res 2021 Apr 16;194:116938. Epub 2021 Feb 16.

Key Laboratory of Building Safety and Energy Efficiency, Ministry of Education, Department of Water Engineering and Science, College of Civil Engineering, Hunan University, Changsha, 410082, China. Electronic address:

The UV/monochloramine (NHCl) process is an advanced oxidation process that can effectively remove emerging contaminants (ECs). However, the degradation mechanisms of reactive radicals with ECs are not clear. In this work, we combined theoretical calculations with experimental studies to investigate the kinetics and mechanism of radical-mediated degradation of paracetamol (AAP) in UV/NHCl process. The degradation of AAP in UV/NHCl process accords with the pseudo first-order kinetics. Impact factors including NHCl dose, pH, natural organic matter, HCO, and NO were evaluated. The reaction mechanisms of AAP with hydroxyl radical (HO), reactive chlorine species (RCS), and reactive nitrogen species (RNS) were discussed in detail. Specifically, HO attacked AAP mainly through hydrogen atom transfer (HAT) and radical adduct formation (RAF), while Cl play a certain role through single electron transfer (SET). NH and Cl destructed AAP mainly through HAT. Based on the mechanism analysis, the second-order rate constants of AAP reacts with HO, Cl, NH, ClO, Cl and NO were calculated through transition state theory as 2.66×10 M s, 2.61×10 M s, 1.02×10 M s, 7.74×10 M s, 1.32×10 M s, 1.48×10 M s respectively. The second-order rate constants were then used to distinguish the contribution of radicals to the degradation of AAP. Thirteen transformation products were identified by high-resolution mass spectrometry. Combined active sites with potential energy surface, the detailed reaction pathways were proposed. Overall, this study provides deep insights into the mechanism of radical-mediated degradation of AAP.
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http://dx.doi.org/10.1016/j.watres.2021.116938DOI Listing
April 2021

Exploring interactions between lipids and amyloid-forming proteins: A review on applying fluorescence and NMR techniques.

Chem Phys Lipids 2021 May 15;236:105062. Epub 2021 Feb 15.

National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, PR China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, PR China. Electronic address:

A hallmark of Alzheimer's, Parkinson's, and other amyloid diseases is the assembly of amyloid proteins into amyloid aggregates or fibrils. In many cases, the formation and cytotoxicity of amyloid assemblies are associated with their interaction with cell membranes. Despite studied for many years, the characterization of the interaction is challenged for reasons on the multiple aggregation states of amyloid-forming proteins, transient and weak interactions in the complex system. Although several strategies such as computation biology, spectroscopy, and imaging methods have been performed, there is an urgent need to detail the molecular mechanism in different time scales and high resolutions. This review highlighted the recent applications of fluorescence, solution and solid-state NMR in exploring the interactions between amyloid protein and membranes attributing to their advantages of high sensitivity and atomic resolution.
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http://dx.doi.org/10.1016/j.chemphyslip.2021.105062DOI Listing
May 2021

Integration of transcriptomic and proteomic analyses for finger millet [Eleusine coracana (L.) Gaertn.] in response to drought stress.

PLoS One 2021 17;16(2):e0247181. Epub 2021 Feb 17.

Agricultural College, Hunan Agricultural University, Changsha, Hunan, China.

Drought is one of the most significant abiotic stresses that affects the growth and productivity of crops worldwide. Finger millet [Eleusine coracana (L.) Gaertn.] is a C4 crop with high nutritional value and drought tolerance. However, the drought stress tolerance genetic mechanism of finger millet is largely unknown. In this study, transcriptomic (RNA-seq) and proteomic (iTRAQ) technologies were combined to investigate the finger millet samples treated with drought at different stages to determine drought response mechanism. A total of 80,602 differentially expressed genes (DEGs) and 3,009 differentially expressed proteins (DEPs) were identified in the transcriptomic and proteomic levels, respectively. An integrated analysis, which combined transcriptome and proteome data, revealed the presence of 1,305 DEPs were matched with the corresponding DEGs (named associated DEGs-DEPs) when comparing the control to samples which were treated with 19 days of drought (N1-N2 comparison group), 1,093 DEGs-DEPs between control and samples which underwent rehydration treatment for 36 hours (N1-N3 comparison group) and 607 DEGs-DEPs between samples which were treated with drought for 19 days and samples which underwent rehydration treatment for 36 hours (N2-N3 comparison group). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 80 DEGs-DEPs in the N1-N2 comparison group, 49 DEGs-DEPs in the N1-N3 comparison group, and 59 DEGs-DEPs in the N2-N3 comparison group, which were associated with drought stress. The DEGs-DEPs which were drought tolerance-related were enriched in hydrolase activity, glycosyl bond formation, oxidoreductase activity, carbohydrate binding and biosynthesis of unsaturated fatty acids. Co-expression network analysis revealed two candidate DEGs-DEPs which were found to be centrally involved in drought stress response. These results suggested that the coordination of the DEGs-DEPs was essential to the enhanced drought tolerance response in the finger millet.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247181PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888627PMC
February 2021

Biosafety risk assessment of nanoparticles: Evidence from food case studies.

Environ Pollut 2021 Apr 4;275:116662. Epub 2021 Feb 4.

National Engineering Laboratory for Deep Process of Rice and Byproducts, Hunan Province Key Laboratory of Edible Forestry Resources Safety and Processing Utilization, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China.

Nanotechnology provides a wide range of benefits in the food industry in improving food tastes, textures, sensations, quality, shelf life, and food safety. Recently, potential adverse effects such as toxicity and safety concerns have been associated with the increasing use of engineered nanoparticles in food industry. Additionally, very limited information is known concerning the behavior, properties and effects of food nano-materials in the gastrointestinal tract. There is explores the current advances and provides insights of the potential risks of nanoparticles in the food industry. Specifically, characteristics of food nanoparticles and their absorption in the gastrointestinal tract, the effects of food nanoparticles against the gastrointestinal microflora, and the potential toxicity mechanisms in different organs and body systems are discussed. This review would provide references for further investigation of nano-materials toxicity effect in foods and their molecular mechanisms. It will help to develop safer foods and expand nano-materials applications in safe manner.
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http://dx.doi.org/10.1016/j.envpol.2021.116662DOI Listing
April 2021
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