Publications by authors named "Jing Chen"

6,088 Publications

  • Page 1 of 1

Suppression of FAM83D Inhibits Glioma Proliferation, Invasion and Migration by Regulating the AKT/mTOR Signaling Pathway.

Transl Oncol 2022 May 23;22:101454. Epub 2022 May 23.

Center for Diagnosis and Treatment of Neuro-oncology Diseases, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China. Electronic address:

Objective: To explore the mechanism by which the family with sequence similarity 83, member D (FAM83D)-mediated AKT/mTOR signaling pathway activation affects the proliferation and metastasis of glioma cells.

Methods: FAM83D protein expression in glioma cells and tissues was detected by western blotting. Glioma U87 and U251 cells were selected and divided into the Mock, siNC, siFAM83D, FAM83D, MK2206 and FAM83D + MK2206 groups. Cell proliferation was assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) and clone formation assays, while invasion and migration were evaluated by Transwell assays and wound healing tests. The protein expression of members of the AKT/mTOR pathway was determined via western blotting. Xenograft models were also established in nude mice to observe the in vivo effect of FAM83D on the growth of glioma.

Results: FAM83D was upregulated in glioma patients, especially in those with Stage III-IV. In addition, cells treated with siFAM83D had significant downregulation of p-AKT/AKT and p-mTOR/mTOR, with decreased proliferation and colony numbers, as well as decreased invasion and migration compared to the Mock group. However, FAM83D overexpression could activate the Akt/mTOR pathway and promote the proliferation, invasion and migration of glioma cells. Moreover, treatment with MK2206, an inhibitor of AKT, reversed the promoting effect of FAM83D on the growth of glioma cells. The in vivo experiments demonstrated that silencing FAM83D could inhibit the in vivo growth of glioma cells CONCLUSION: FAM83D was upregulated in glioma and silencing FAM83D suppressed the proliferation, invasion and migration of glioma cells via inhibition of the AKT/mTOR pathway.
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http://dx.doi.org/10.1016/j.tranon.2022.101454DOI Listing
May 2022

Hydrotrioxide (ROOOH) formation in the atmosphere.

Science 2022 May 26;376(6596):979-982. Epub 2022 May 26.

Department of Chemistry, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark.

Organic hydrotrioxides (ROOOH) are known to be strong oxidants used in organic synthesis. Previously, it has been speculated that they are formed in the atmosphere through the gas-phase reaction of organic peroxy radicals (RO) with hydroxyl radicals (OH). Here, we report direct observation of ROOOH formation from several atmospherically relevant RO radicals. Kinetic analysis confirmed rapid RO + OH reactions forming ROOOH, with rate coefficients close to the collision limit. For the OH-initiated degradation of isoprene, global modeling predicts molar hydrotrioxide formation yields of up to 1%, which represents an annual ROOOH formation of about 10 million metric tons. The atmospheric lifetime of ROOOH is estimated to be minutes to hours. Hydrotrioxides represent a previously omitted substance class in the atmosphere, the impact of which needs to be examined.
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http://dx.doi.org/10.1126/science.abn6012DOI Listing
May 2022

Highly Elastic, Sensitive, Stretchable, and Skin-Inspired Conductive Sodium Alginate/Polyacrylamide/Gallium Composite Hydrogel with Toughness as a Flexible Strain Sensor.

Biomacromolecules 2022 May 26. Epub 2022 May 26.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 North Third Ring Road East, Chaoyang District, Beijing 100029, P. R. China.

As a classic flexible material, hydrogels show great potential in wearable electronic devices. The application of strain sensors prepared using them in human health monitoring and humanoid robotics is developing rapidly. However, it is still a challenge to fabricate a high-toughness, large-tensile-deformation, strain-sensitive. and human-skin-fit hydrogel with the integration of excellent mechanical properties and high electrical conductivity. In this study, a flexible sensor using a highly strain-sensitive skin-like hydrogel with acrylamide and sodium alginate was designed using liquid metallic gallium as a "reactive" conductive filler. The sensor had a low elastic modulus (30 kPa) similar to that of skin, a high-toughness (2.25 MJ m), self-stiffness, a large tensile deformation (1400%), recoverability, and excellent fatigue resistance. Moreover, the addition of gallium might enhance the electrical conductivity (1.9 S m) of the hydrogel while maintaining high transparency, and the flexible sensor device constructed from it showed high sensitivity to strain (gauge factor = 4.08) and pressure (gauge factor = 0.455 kPa). As a result, the hydrogel sensor could monitor various human motions, including large-scale joint bending and tiny facial expression, breathing, voice recognition, and handwriting. Furthermore, it might even be used for human-computer communication.
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http://dx.doi.org/10.1021/acs.biomac.2c00329DOI Listing
May 2022

Double-type-I charge-injection heterostructure for quantum-dot light-emitting diodes.

Mater Horiz 2022 May 26. Epub 2022 May 26.

Department of Physics, National University of Singapore, Lower Kent Ridge Road, S117550, Singapore.

Enforcing balanced electron-hole injection into the emitter layer of quantum-dot light-emitting diodes (QLEDs) remains key to maximizing the quantum efficiency over a wide current density range. This was previously thought not possible for quantum dot (QD) emitters because of their very deep energy bands. Here, we show using Mesolight® blue-emitting CdZnSeS/ZnS QDs as a model that its valence levels are in fact considerably shallower than the corresponding band maximum of the bulk semiconductor, which makes the ideal double-type-I injection/confinement heterostructure accessible using a variety of polymer organic semiconductors as transport and injection layers. We demonstrate flat external quantum efficiency characteristics that indicate near perfect recombination within the QD layer over several decades of current density from the onset of device turn-on of about 10 μA cm, for both normal and inverted QLED architectures. We also demonstrate that these organic semiconductors do not chemically degrade the QDs, unlike the usual ZnMgO nanoparticles. However, these more efficient injection heterostructures expose a new vulnerability of the QDs to electrochemical degradation. The work here opens a clear path towards next-generation ultra-high-performance, all-solution-processed QLEDs.
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http://dx.doi.org/10.1039/d1mh00859eDOI Listing
May 2022

Clinical Value of Serum Neuron-Specific Enolase Combined with Serum S100B Protein in the Diagnosis of Systemic Lupus Erythematosus.

Contrast Media Mol Imaging 2022 9;2022:9390991. Epub 2022 May 9.

Department of Pathogenic Biology, Hebei Medical University, Shijiazhuang 050082, China.

Objective: To investigate the clinical value of serum neuron-specific enolase (NSE) combined with serum S100B protein in the diagnosis of systemic lupus erythematosus (SLE).

Methods: Sixty patients with SLE treated in our hospital from January 2019 to April 2021 were enrolled as the study group. According to the degree of activity, the study group was assigned into three groups: mild activity group ( = 20), moderate activity group ( = 20), and severe activity group ( = 20). A total of 60 healthy people who underwent physical examination in our hospital in the same period were enrolled as the control group. The NSE and serum S100B protein were detected in the two groups, and the correlation between serum nerve-specific enolase and serum S100B protein and the clinical value in the diagnosis of SLE were analyzed.

Results: First of all, we compared the general data of the two groups. There was no significant difference in sex, age, marital status, and education level, and no significant difference was exhibited ( > 0.05). There was no significant difference in sex, age, marital status, and education level among mild activity group, moderate activity group, and severe activity group, and no significant difference in data was exhibited ( > 0.05). Secondly, we compared the levels of serum S100B protein and NSE. The levels of serum S100B protein and NSE in the study group were higher compared to the control group ( < 0.05). The levels of serum S100B protein and NSE in patients with different activity levels of SLE were compared. The levels of serum S100B protein and NSE in mild activity group < moderate activity group < severe activity group were significantly different ( < 0.05). Correlation analysis between serum S100B, NSE levels, and SLE activity indicated that serum S100B and NSE levels were positively correlated with SLE activity. With the increase of SLE activity, serum S100B and NSE levels gradually increased, and the data difference was statistically significant ( = 0.855, 0.844,  < 0.05). Finally, we established the logistic prediction model, take the probability of generating prediction as the analysis index, and draw the ROC curve to evaluate the diagnostic value of different combinations to SLE. The highest AUC and sensitivity of the two indexes in the diagnosis of SLE were 0.773 and 0.836, respectively. The levels of serum S100B protein and NSE have a certain value in the diagnosis of SLE, while the combined diagnosis is of higher value, sensitivity, and specificity in the diagnosis of SLE.

Conclusion: Serum S100B protein and NSE are very sensitive indexes to judge the damage of central nervous system. However, due to the small number of cases in this study, there were as many as 19 kinds of NPSLE classification, so the relationship between serum S100B protein, NSE levels, and various NPSLE and their exact application value in diagnosing the disease and judging the prognosis needs to be confirmed by expanding the number of cases.
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http://dx.doi.org/10.1155/2022/9390991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110203PMC
May 2022

Surface-Initiated ARGET ATRP of Poly(Glycidyl Methacrylate) from Carbon Nanotubes via Bioinspired Catechol Chemistry for Efficient Adsorption of Uranium Ions.

ACS Macro Lett 2016 Mar 26;5(3):382-386. Epub 2016 Feb 26.

Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, United States.

Surface-initiated activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) integrated with mussel-inspired polydopamine (PDA) chemistry was, for the first time, employed for controlled grafting of poly(glycidyl methacrylate) (PGMA) brushes from carbon nanotubes (CNTs). The strategy initially involved deposition of a PDA layer by spontaneous self-polymerization, which is a benign and nonsurface specific way for anchoring 2-bromoisobutyryl bromide to form initiators on the CNTs. Dense and uniform PGMA brushes were then grown via ARGET ATRP using low concentration of Cu catalyst in different solvents. With abundant highly reactive epoxy groups, the PGMA-grafted CNTs could serve as a versatile platform for further modification or functionalization. Ethylenediamine ligands were facilely introduced, imparting the functionalized CNTs with record-high adsorption ability toward uranium ions among CNTs composites. The integrated strategy combining surface-initiated ARGET ATRP technique and PDA chemistry would provide new opportunities for surface engineering of nanomaterials for advanced applications.
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http://dx.doi.org/10.1021/acsmacrolett.6b00099DOI Listing
March 2016

Advances of microRNAs in regulating mitochondrial function: new potential application in NAFLD treatment.

Mol Biol Rep 2022 May 25. Epub 2022 May 25.

Center for Drug Safety Evaluation and Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai, 201203, China.

Nonalcoholic fatty liver disease (NAFLD) is one of the most common metabolic diseases and closely associated with lipid disorder. Mitochondrion has been recognized to play a key role in lipid metabolism as the main site of energy metabolism in cells, and its dysfunction is involved in the progression of NAFLD. MicroRNAs (miRNAs), one of regulators in the pathogenesis of NAFLD, are discovered to modulate mitochondrial function by targeting mitochondrial proteins or mitochondrial-related factors, thereby improving or deteriorating NAFLD-associated pathologies. This review summarizes the differentially expressed miRNAs from clinical and experimental models of NAFLD with abilities in regulating mitochondrial function, expounds their underlying molecular mechanism and discusses their prospect and future research direction.
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http://dx.doi.org/10.1007/s11033-022-07503-0DOI Listing
May 2022

Electrically Modulated Near-Infrared/Visible Light Dual-Mode Perovskite Photodetectors.

ACS Appl Mater Interfaces 2022 May 25. Epub 2022 May 25.

Joint International Research Laboratory of Information Display and Visualization, School of Electronic Science and Engineering, Southeast University, Nanjing 210096, China.

Dual-mode photodetectors (PDs) have attracted increasing interest owing to their potential optoelectrical applications. However, the widespread use of PDs is still limited by the high cost of epitaxial semiconductors. In contrast, the solution processability and wide spectral tunability of perovskites have led to the development of various inexpensive and high-performance optoelectronic devices. In this study, we develop a high-performance electronically modulated dual-mode PD with near-infrared (NIR) narrowband and visible light broadband detection based on organic-inorganic hybrid methylammonium lead halide perovskite (MAPbX; MA = CHNH and X = Cl, Br, and I) single crystals with a pnp configuration. The operating mode of the dual-mode PD can be switched according to voltage bias polarity because the photon absorption region and carrier transport performance are tuned at different bias voltages. The dual-mode PD exhibits a NIR light responsivity of 0.244 A/W and a narrow full width at half-maximum of ∼12 nm at 820 nm at positive voltages and an average visible light responsivity of ∼0.13 A/W at negative voltages. The detectivities of both modes are high (∼10 Jones), and the linear dynamic range is wide (>100 dB). Our study provides a new method for fabricating multifunctional PDs and can expand their application in integrated imaging systems.
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http://dx.doi.org/10.1021/acsami.2c01796DOI Listing
May 2022

Two-Stage Targeted Bismuthene-Based Composite Nanosystem for Multimodal Imaging Guided Enhanced Hyperthermia and Inhibition of Tumor Recurrence.

ACS Appl Mater Interfaces 2022 May 24. Epub 2022 May 24.

Department of Pharmacology, School of Basic Medical Sciences, Xi'an Medical University, Xi'an, Shaanxi 710021, China.

A key challenge for nanomedicines in clinical application is to reduce the dose while achieving excellent efficacy, which has attracted extensive attention in dose toxicity and potential risks. It is thus necessary to reasonably design nanomedicine with high-efficiency targeting and accumulation. Here, we designed and synthesized a tetragonal bismuthene-based "all-in-one" composite nanosystem ([email protected]@CP) with two-stage targeting, multimodal imaging, photothermal therapy, and immune enhancement functions. Through the elaborate design of its structure, the composite nanosystem possesses multiple properties including (i) two-stage targeting function of hepatoma cells and mitochondria [the aggregation at the tumor site is 2.63-fold higher than that of traditional enhanced permeability and retention (EPR) effect]; (ii) computed tomography (CT) contrast-enhancement efficiency as high as ∼51.8 HU mL mg (3.16-fold that of the clinically available iopromide); (iii) ultrahigh photothermal conversion efficiency (52.3%, 808 nm), promising photothermal therapy (PTT), and high-contrast infrared thermal (IRT)/photoacoustic (PA) imaging of tumor; (iv) benefitting from the two-stage targeting function and excellent photothermal conversion ability, the dose used in this strategy is one of the lowest doses in hyperthermia (the inhibition rate of tumor cells was 50% at a dose of 15 μg mL and 75% at a dose of 25 μg mL); (v) the compound polysaccharide (CP) shell with hepatoma cell targeting and immune enhancement functions effectively inhibited the recurrence of tumor. Therefore, our work reduces the dose toxicity and potential risk of nanomedicines and highlights the great potential as an all-in-one theranostic nanoplatform for two-stage targeting, integrated diagnostic imaging, photothermal therapy, and inhibition of tumor recurrence.
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http://dx.doi.org/10.1021/acsami.2c01128DOI Listing
May 2022

Separation of Americium from Curium through Oxidation State Control with Record Efficiency.

Anal Chem 2022 May 24. Epub 2022 May 24.

Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, P. R. China.

Separation of americium (Am) from curium (Cm) could greatly facilitate the development of advanced nuclear fuel cycles, help with accurate nuclear forensic analysis, and allow for more efficient recovery and utilization of the two strategic elements. In this work, an Am/Cm separation strategy based on the stabilization of pentavalent Am in a biphasic solvent extraction system using bismuthate as the oxidant and -tetraoctyl diglycolamide (TODGA) as the extractant was developed. The distinctive differences between Am(V) and Cm(III) in terms of both steric configuration and charge density afford record high Cm/Am separation factors (>10) through only one single contact in solvent extraction. This separation strategy possesses superior features including fast kinetics, long-time stability, no secondary solid radioactive waste generation, and applicability over a wide range of HNO acidities, making it promising for Am/Cm separation and analysis in a variety of applications.
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http://dx.doi.org/10.1021/acs.analchem.2c01492DOI Listing
May 2022

Peripheral Blood Inflammatory Cytokines are Associated with Rapid Eye Movement Sleep Behavior Disorder in Parkinson's Disease.

Neurosci Lett 2022 May 20:136692. Epub 2022 May 20.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu 215004, China. Electronic address:

Objective: Previous studies have shown the essential role of inflammation in rapid eye movement (REM) sleep behavior disorder (RBD). However, the association of RBD in Parkinson's disease (PD) with peripheral blood inflammatory cytokines is still unknown. We investigated the relationship between inflammatory cytokines and the clinical characteristics of PD patients with RBD.

Methods: A total of 153 PD patients and 36 healthy controls were included in this study, and blood plasma was collected. PD patients were classified as PD with RBD (PD-RBD, n=60) and PD without RBD (PD-nRBD, n=93). Inflammatory factor levels were compared among the control, PD-RBD, and PD-nRBD groups.

Results: The PD-RBD group had significantly higher C-reactive protein (CRP) levels (P<0.001), monocytes (P=0.003), and neutrophil-to-lymphocyte ratio (NLR) (P<0.001), whereas this group has lower lymphocytes levels (P<0.001) and lymphocyte-to-monocyte ratio (LMR) (P<0.001) than the PD-nRBD group. Univariate and multivariate logistic regression analysis indicated that LMR (P<0.0001 odds ratio [OR]=0.424) was a protective factor, whereas CRP (P<0.001 OR=2.326) was a risk factor for the PD-RBD group. PD-RBD patients had lower Montreal Cognitive Assessment (Beijing version) (MoCA) (P<0.001) and Mini-Mental State Examination (MMSE) (P=0.039) scores than PD-nRBD patients.

Conclusions: Significant differences were found in inflammation levels between PD-RBD and PD-nRBD, suggesting that inflammatory factors are associated with the pathogenesis of RBD in PD patients. Thus, CRP and LMR levels may serve as biomarkers and predict the prognosis of PD patients with RBD.
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http://dx.doi.org/10.1016/j.neulet.2022.136692DOI Listing
May 2022

A Spatial and Geometry Feature-Based Quality Assessment Model for the Light Field Images.

IEEE Trans Image Process 2022 May 23;PP. Epub 2022 May 23.

This paper proposes a new full-reference image quality assessment (IQA) model for performing perceptual quality evaluation on light field (LF) images, called the spatial and geometry feature-based model (SGFM). Considering that the LF image describe both spatial and geometry information of the scene, the spatial features are extracted over the sub-aperture images (SAIs) by using contourlet transform and then exploited to reflect the spatial quality degradation of the LF images, while the geometry features are extracted across the adjacent SAIs based on 3D-Gabor filter and then explored to describe the viewing consistency loss of the LF images. These schemes are motivated and designed based on the fact that the human eyes are more interested in the scale, direction, contour from the spatial perspective and viewing angle variations from the geometry perspective. These operations are applied to the reference and distorted LF images independently. The degree of similarity can be computed based on the above-measured quantities for jointly arriving at the final IQA score of the distorted LF image. Experimental results on three commonly-used LF IQA datasets show that the proposed SGFM is more in line with the quality assessment of the LF images perceived by the human visual system (HVS), compared with multiple classical and state-of-the-art IQA models.
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http://dx.doi.org/10.1109/TIP.2022.3175619DOI Listing
May 2022

Identification of Angiogenesis-Related Prognostic Biomarkers Associated With Immune Cell Infiltration in Breast Cancer.

Front Cell Dev Biol 2022 6;10:853324. Epub 2022 May 6.

Department of Breast Cancer Center, Chongqing University Cancer Hospital, Chongqing, China.

This study aimed to explore the prognostic value of angiogenesis-related genes (ARGs) and their association with immune cell infiltration (ICI) in breast cancer (BC). Transcriptome data of BC were obtained from the TCGA and GEO databases. Differentially expressed ARGs were identified by the limma package. The identification of key genes and construction of the risk score model were performed by univariate and multivariate Cox regression algorithms. The prognostic value of the risk score was assessed by ROC curves and nomogram. GO, KEGG pathway, and GSEA were used to investigate the biological functions of differentially expressed genes (DEGs), and CIBERSORT, ssGSEA, and xCell algorithms were performed to estimate the ICI in high-risk and low-risk groups. The correlations between prognostic biomarkers and differentially distributed immune cells were assessed. Moreover, a ceRNA regulatory network based on prognostic biomarkers was constructed and visualized by Cytoscape software. A total of 18 differentially expressed ARGs were identified between tumor and adjacent normal tissue samples. TNFSF12, SCG2, COL4A3, and TNNI3 were identified as key prognostic genes by univariate and multivariate Cox regression analyses. The risk score model was further constructed based on the four-gene signature and validated in GSE7390 and GSE88770 datasets. ROC curves and nomogram indicated that the risk score had good accuracy for determining BC patient survival. Biological function analysis showed that DEGs in high- and low-risk groups had a high enrichment in immune-related biological processes and signaling pathways. Moreover, significantly different ICIs were found between high- and low-risk groups, such as memory B cells, CD8 T cells, resting memory CD4 T cells, follicular helper T cells, regulatory T cells, monocytes, M2 macrophages, and neutrophils, and each prognostic biomarker was significantly correlated with one or more immune cell types. The current study identified novel prognostic ARGs and developed a prognostic model for predicting survival in patients with BC. Furthermore, this study indicated that ICI may act as a bond between angiogenesis and BC. These findings enhance our understanding of angiogenesis in BC and provide novel guidance on developing therapeutic targets for BC patients.
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http://dx.doi.org/10.3389/fcell.2022.853324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121305PMC
May 2022

Flagellar Motor Transformed: Biophysical Perspectives of the Gliding Mechanism.

Front Microbiol 2022 6;13:891694. Epub 2022 May 6.

Department of Biology, Texas A&M University, College Station, TX, United States.

Many bacteria move on solid surfaces using gliding motility, without involvement of flagella or pili. Gliding of is powered by a proton channel homologous to the stators in the bacterial flagellar motor. Instead of being fixed in place and driving the rotation of a circular protein track like the flagellar basal body, the gliding machinery of travels the length of the cell along helical trajectories, while mechanically engaging with the substrate. Such movement entails a different molecular mechanism to generate propulsion on the cell. In this perspective, we will discuss the similarities and differences between the gliding machinery and bacterial flagellar motor, and use biophysical principles to generate hypotheses about the operating mechanism, efficiency, sensitivity to control, and mechanosensing of gliding.
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http://dx.doi.org/10.3389/fmicb.2022.891694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120999PMC
May 2022

Identification of Potential WSB1 Inhibitors by AlphaFold Modeling, Virtual Screening, and Molecular Dynamics Simulation Studies.

Evid Based Complement Alternat Med 2022 13;2022:4629392. Epub 2022 May 13.

Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, Jiangsu, China.

WD40 repeat and SOCS box containing 1 (WSB1) consists of seven WD40 repeat structural domains at the -terminal end and one SOCS box structural domain at the -terminal end. WSB1 promotes cancer progression by affecting the Von Hippel-Lindau tumor suppressor protein (pVHL) and upregulating hypoxia inducible factor-1 (HIF-1) target gene expression. However, the crystal structure of WSB1 has not been reported, which is not beneficial to the research on WSB1 inhibitors. Therefore, we focused on specific small molecule inhibitors of WSB1. This study applied virtual screening and molecular dynamics simulations; finally, 20 compounds were obtained. Among them, compound G490-0341 showed the best stable structure and was a promising composite for further development of WSB1 inhibitors.
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http://dx.doi.org/10.1155/2022/4629392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122669PMC
May 2022

Blood Metabolomics Analysis Identifies Differential Serum Metabolites in Elite and Sub-elite Swimmers.

Front Physiol 2022 5;13:858869. Epub 2022 May 5.

College of Rehabilitation Sciences, Shanghai University of Medicine and Health Sciences, Shanghai, China.

Metabolites in body fluids, such as lactate, glucose, and creatinine, have been measured by conventional methods to evaluate physical function and performance or athletic status. The objectives of the current study were to explore the novel metabolite biomarkers in professional swimmers with different competition levels using nuclear magnetic resonance (NMR) metabolomics, and try to establish a model to identify the athletic status or predict the competitive potential. Serum samples were collected from 103 elite and 84 sub-elite level Chinese professional swimmers, and were profiled by NMR analysis. Out of the thirty-six serum metabolites profiled, ten were associated with the athletic status of swimmers (with < 0.05). When compared with sub-elite swimmers, elite swimmers had higher levels of high-density lipoprotein (HDL), unsaturated fatty acid, lactic acid, and methanol. Elite swimmers had lower levels of isoleucine, 3-hydroxybutyric acid, acetoacetate, glutamine, glycine, and α-glucose. A model with four metabolites, including HDL, glutamine, methanol, and α-glucose, was established to predict athletic status by adjusting with different covariates. The area under the curve (AUC) of the best model was 0.904 (95% CI: 0.862-0.947), with a sensitivity and specificity of 75.5 and 90.2%, respectively. We have identified ten metabolite biomarkers with differentially expressed levels between elite and sub-elite swimmers, the differences could result from genetic or sports level between the two cohorts. A model with four metabolites has successfully differentiated professional swimmers with different competitive levels.
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http://dx.doi.org/10.3389/fphys.2022.858869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118345PMC
May 2022

Decreasing integration within face network and segregation beyond the face network in the aging brain.

Psych J 2022 May 22. Epub 2022 May 22.

Center for Cognition and Brain Disorders, The Affiliated Hospital of Hangzhou Normal University, Zhejiang, China.

Face processing is known to decline in older adults; however, a clear understanding of the brain networks behind this cognitive decline is still lacking. In this study, we investigated the neural correlates of the declined face processing with aging from a resting-state brain network perspective. Nineteen healthy old adults and 22 young adults were recruited and underwent two functional magnetic resonance imaging (fMRI) scanning sessions (i.e., resting-state and localizer task) and two behavioral tests (face matching and symbol-form matching). We examined age-related alterations in resting-state functional connectivity (FC) within face network as well as between face network and other networks, and tested their associations with behavioral performance of face and symbol-form processing. We found that (a) compared with young adults, old adults exhibited decreased FC between face-selective regions (fusiform face area and occipital face area), but increased FC between face-selective regions and non-face-selective regions; (b) these age-related FC alterations were correlated with individuals' behavioral performance of face and symbol-form processing. Collectively, these findings suggest the declines of face processing are associated with a mixture of decreased integration within the face network and segregation beyond the face network in the aging brain, and provide evidence for a neural basis of cognitive aging in face processing from an intrinsic brain network perspective.
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http://dx.doi.org/10.1002/pchj.560DOI Listing
May 2022

Metabolomics study of blood pressure salt-sensitivity and hypertension.

Nutr Metab Cardiovasc Dis 2022 Apr 11. Epub 2022 Apr 11.

Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA, United States. Electronic address:

Background And Aims: Identify novel metabolite associations with blood pressure (BP) salt-sensitivity and hypertension.

Methods And Results: The Genetic Epidemiology Network of Salt Sensitivity (GenSalt) Replication study includes 698 Chinese participants who underwent a 3-day baseline examination followed by a 7-day low-sodium feeding and 7-day high-sodium feeding. Latent mixture models identified three trajectories of blood pressure (BP) responses to the sodium interventions. We selected 50 most highly salt-sensitive and 50 most salt-resistant participants for untargeted metabolomics profiling. Multivariable adjusted mixed logistic regression models tested the associations of baseline metabolites with BP salt-sensitivity. Multivariable adjusted mixed linear regression models tested the associations of BP salt-sensitivity with metabolite changes during the sodium interventions. Identified metabolites were tested for associations with hypertension among 1249 Bogalusa Heart Study (BHS) participants using multiple logistic regression. Fifteen salt-sensitivity metabolites were associated with hypertension in the BHS. Baseline values of serine, 2-methylbutyrylcarnitine and isoleucine directly associated with high salt-sensitivity. Among them, serine indirectly associated with hypertension while 2-methylbutyrylcarnitine and isoleucine directly associated with hypertension. Baseline salt-sensitivity status predicted changes in 14 metabolites when switching to low-sodium or high-sodium interventions. Among them, glutamate, 1-carboxyethylvaline, 2-methylbutyrylcarnitine, 3-methoxytyramine sulfate, glucose, alpha-ketoglutarate, hexanoylcarnitine, gamma-glutamylisoleucine, gamma-glutamylleucine, and gamma-glutamylphenylalanine directly associated with hypertension. Conversely, serine, histidine, threonate and 5-methyluridine indirectly associated with hypertension. Together, these metabolites explained an additional 7% of hypertension susceptibility when added to a model including traditional risk factors.

Conclusions: Our findings contribute to the molecular characterization of BP response to sodium and provide novel biological insights into salt-sensitive hypertension.
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http://dx.doi.org/10.1016/j.numecd.2022.04.002DOI Listing
April 2022

Channel Coupling Dynamics of Deep-Lying Orbitals in Molecular High-Harmonic Generation.

Phys Rev Lett 2022 May;128(18):183202

Institute of Applied Physics and Computational Mathematics, P.O. Box 8009, Beijing 100088, China.

Investigation on structures in the high-harmonic spectrum has provided profuse information of molecular structure and dynamics in intense laser fields, based on which techniques of molecular ultrafast dynamics imaging have been developed. Combining ab initio calculations and experimental measurements on the high-harmonic spectrum of the CO_{2} molecule, we find a novel dip structure in the low-energy region of the harmonic spectrum which is identified as fingerprints of participation of deeper-lying molecular orbitals in the process and decodes the underlying attosecond multichannel coupling dynamics. Our work sheds new light on the ultrafast dynamics of molecules in intense laser fields.
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http://dx.doi.org/10.1103/PhysRevLett.128.183202DOI Listing
May 2022

Naringin in the repair of knee cartilage injury via the TGF-β/ALK5/Smad2/3 signal transduction pathway combined with an acellular dermal matrix.

J Orthop Translat 2022 Jan 6;32:1-11. Epub 2021 Aug 6.

Orthopedics Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

Objective: Based on the expression changes in the TGF-β/ALK5/Smad2/3 signal transduction pathway, the repair of cartilage injury in the rabbit knee joint was investigated and evaluated by oral administration of naringin in combination with acellular dermal matrix implantation.

Methods: First, twenty New Zealand white rabbits were randomly divided into five groups: a sham operation group (Sham group), a model group (Mod group), a naringin group (Nar group), an acellular dermal matrix group (ADM group), a naringin ​+ ​acellular dermal matrix group (Nar/ADM group). After the 12th week, the repaired tissues were assessed for histomorphology and repair content of the repaired site by observing the morphological characteristics of articular cartilage. The International Cartilage Repair Society (ICRS)'s macroscopic evaluation of the cartilage repair scale and the quantitative scoring repair effect of the modified O'Driscoll grading system were used as evaluation criteria. In addition, the structure of the rabbit knee joint was evaluated by micro-CT scan, histological staining (H & E staining, Alcian blue staining, Safranin-O staining) and immunohistochemical staining (TGF-β2 immunostaining, TGF-β3 immunostaining, Sox-9 immunostaining).

Results: ① The observation of the repair morphology of joint defect tissues showed that the repair effects of the Nar and ADM groups were better than that of the Mod group, and the repair effect of Nar/ADM group was the best ( < 0.05). ② Quantitative scoring of joint defect tissue showed that the Nar/ADM group had the best repair efficacy in the quantitative scores of the above two scales compared with the other groups ( < 0.05). ③ Micro-CT scan showed that the ADM group had obvious repair of the defect structure, while the ADM/Nar group had blurred repair boundaries, and the layers of cartilage and subchondral bone were clear. ④ Histological staining (H & E staining, Alcian blue stain, Safranin-O staining) showed that the ADM group had a better effect on the repair of joint structure at the joint defect, the Nar group had a better effect on the repair of cartilage quality at the joint defect, and the ADM/Nar group had satisfactory results in both of the above aspects. ⑤ Immunohistochemical staining (TGF-β2 immunostaining, TGF-β3 immunostaining, Sox-9 immunostaining) revealed that the Nar group showed more abundant expression of the above proteins in articular cartilage defects than the Mod and ADM groups and that the Nar/ADM groups showed extensive TGF-β2, TGF-β3 and Sox-9 protein expression, with uniform expression and smooth distribution.

Conclusions: Oral administration of naringin, the active ingredient of Rhizoma Drynariae, combined with acellular dermal matrix can achieve better repair effects in both joint structure repair and cartilage quality repair at the defect site when repairing cartilage defects in rabbit knees, and the generation of this effect may be caused by the activation of the TGF-β/ALK5/Smad2/3 signal transduction pathway by naringin, resulting in the increased expression of TGF-β2, TGF-β3, and Sox-9 in cartilage defects.

The Translational Potential Of This Article: Naringin combined with acellular dermal matrix can facilitate the repair of osteochondral defects and has potential for application in osteochondral tissue engineering.
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http://dx.doi.org/10.1016/j.jot.2021.06.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072805PMC
January 2022

Mechanical limitation of bacterial motility mediated by growing cell chains.

Biophys J 2022 May 18. Epub 2022 May 18.

Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24060; Fralin Life Sciences Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24060. Electronic address:

Contrasting most known bacterial motility mechanisms, a bacterial sliding motility discovered in at least two Gram-positive bacterial families does not depend on designated motors. Instead, the cells maintain end-to-end connections following cell divisions to form long chains and exploit cell growth and division to push the cells forward. To investigate the dynamics of this motility mechanism, we constructed a mechanical model that depicts the interplay of the forces acting on and between the cells comprising the chain. Due to the exponential growth of individual cells, the tips of the chains can, in principle, accelerate to speeds faster than any known single-cell motility mechanism can achieve. However, analysis of the mechanical model shows that the exponential acceleration comes at the cost of an exponential buildup in mechanical stress in the chain, making overly long chains prone to breakage. Additionally, the mechanical model reveals that the dynamics of the chain expansion hinges on a single non-dimensional parameter. Perturbation analysis of the mechanical model further predicts the critical stress leading to chain breakage and its dependence on the non-dimensional parameter. Finally, we developed a simplistic population expansion model that uses the predicted breaking behavior to estimate the physical limit of chain-mediated population expansion. Predictions from the models provide critical insights into how this motility depends on key physical properties of the cell and the substrate. Overall, our models present a generically applicable theoretical framework for cell chain-mediated bacterial sliding motility and provide guidance for future experimental studies on such motility.
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http://dx.doi.org/10.1016/j.bpj.2022.05.012DOI Listing
May 2022

Quantitative Evaluation System of Wrist Motor Function for Stroke Patients Based on Force Feedback.

Sensors (Basel) 2022 Apr 28;22(9). Epub 2022 Apr 28.

School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Suzhou 215163, China.

Motor function evaluation is a significant part of post-stroke rehabilitation protocols, and the evaluation of wrist motor function helps provide patients with individualized rehabilitation training programs. However, traditional assessment is coarsely graded, lacks quantitative analysis, and relies heavily on clinical experience. In order to objectively quantify wrist motor dysfunction in stroke patients, a novel quantitative evaluation system based on force feedback and machine learning algorithm was proposed. Sensors embedded in the force-feedback robot record the kinematic and movement data of the subject, and the rehabilitation doctor used an evaluation scale to score the wrist function of the subject. The quantitative evaluation models of wrist motion function based on random forest (RF), support vector machine regression (SVR), k-nearest neighbor (KNN), and back propagation neural network (BPNN) were established, respectively. To verify the effectiveness of the proposed quantitative evaluation system, 25 stroke patients and 10 healthy volunteers were recruited in this study. Experimental results show that the evaluation accuracy of the four models is all above 88%. The accuracy of BPNN model is 94.26%, and the Pearson correlation coefficient between model prediction and clinician scores is 0.964, indicating that the BPNN model can accurately evaluate the wrist motor function for stroke patients. In addition, there was a significant correlation between the prediction score of the quantitative assessment system and the physician scale score ( < 0.05). The proposed system enables quantitative and refined assessment of wrist motor function in stroke patients and has the feasibility of helping rehabilitation physicians in evaluating patients' motor function clinically.
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http://dx.doi.org/10.3390/s22093368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101599PMC
April 2022

Overexpression of AHL9 accelerates leaf senescence in Arabidopsis thaliana.

BMC Plant Biol 2022 May 19;22(1):248. Epub 2022 May 19.

State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China.

Background: Leaf senescence, the final stage of leaf growth and development, is regulated by numerous internal factors and environmental cues. Ethylene is one of the key senescence related hormones, but the underlying molecular mechanism of ethylene-induced leaf senescence remains poorly understood.

Results: In this study, we identified one AT-hook like (AHL) protein, AHL9, as a positive regulator of leaf senescence in Arabidopsis thaliana. Overexpression of AHL9 significantly accelerates age-related leaf senescence and promotes dark-induced leaf chlorosis. The early senescence phenotype observed in AHL9 overexpressing lines is inhibited by the ethylene biosynthesis inhibitor aminooxyacetic acid suggesting the involvement of ethylene in the AHL9-associated senescence. RNA-seq and quantitative reverse transcription PCR (qRT-PCR) data identified numerous senescence-associated genes differentially expressed in leaves of AHL9 overexpressing transgenic plants.

Conclusions: Our investigation demonstrates that AHL9 functions in accelerating the leaf senescence process via ethylene synthesis or signalling.
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http://dx.doi.org/10.1186/s12870-022-03622-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118680PMC
May 2022

New sight into interaction between endoplasmic reticulum stress and autophagy induced by vanadium in duck renal tubule epithelial cells.

Chem Biol Interact 2022 May 16:109981. Epub 2022 May 16.

Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, No. 1101 Zhimin Avenue, Economic and Technological Development District, Nanchang, 330045, Jiangxi, PR China.

Vanadium (V) is a common environmental and industrial pollutant that can cause nephrotoxicity in animals in excess. The purpose of this research was to explore the interaction between endoplasmic reticulum (ER) stress and autophagy induced by V in the kidney of ducks. Duck renal tubule epithelial cells were exposed to different concentrations of sodium metavanadate (NaVO) (0, 100 and 200 μM) and PERK inhibitor (GSK, 1 μM), or autophagy inhibitor (chloroquine, 50 μM) alone for 24 h (chloroquine for the last 4 h). The results showed that exposure to V caused the dilatation and swelling of the ER and intracellular calcium overload, and upregulated PERK, eIF2α, ATF4 and CHOP mRNA levels and p-PERK and CHOP protein levels associated with ER stress in cells. Additionally, V markedly increased the number of autophagosomes, acidic vesicular organelles (AVOs) and LC3 puncta, as well as the mRNA levels of Beclin1, Atg5, Atg12, LC3A and LC3B and protein levels of Beclin1, Atg5 and LC3B-II/LC3B-I, but decreased the imRNA and protein levels of p62. Moreover, treatment with the PERK inhibitor ameliorated the changed factors above induced by V, but the V-induced variation of ER-stress related factors were aggravated after treatment with the autophagy inhibitor. Together, our data suggested that excessive V could induce ER stress and autophagy in duck renal tubular epithelial cells. ER stress might promote V-induced autophagy via the PERK/ATF4/CHOP signaling pathway, and autophagy may play a role in alleviating ER stress induced by V.
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http://dx.doi.org/10.1016/j.cbi.2022.109981DOI Listing
May 2022

Application of magnetic hydroxyapatite surface-imprinted polymers in pretreatment for detection of zearalenone in cereal samples.

J Chromatogr B Analyt Technol Biomed Life Sci 2022 May 15;1201-1202:123297. Epub 2022 May 15.

School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, PR China; Key Laboratory of Accurate Separation and Analysis for Complex Matrix of Zhengzhou City, Zhengzhou 450001, PR China.

This study is about the combination of magnetic solid phase extraction (MSPE) and high performance liquid chromatography-fluorescence detector (HPLC-FLD) for the pre-concentration and determination of Zearalenone (ZEA) in grain sample extracts. The novel sorbent ([email protected]), for selective and intelligent extraction of ZEA, was synthesized by doping FeO into the fibrous structure of hydroxyapatite nanoparticle (FeO-HAP) and subsequently wrapping with molecularly imprinted polymers. The characteristic and morphology of magnetic particles were studied by infrared spectroscopy, vibrating sample magnetometer and scanning electron microscopy. The maximum adsorption capacity was 2.89 μg/mg. It could reach the adsorption equilibrium within 5 min. The adsorption isotherm of ZEA by the [email protected] were simulated. The results showed that the extraction process of ZEA with the sorbent accorded with Langmuir isotherm. The important factors affecting the extraction efficiency include elution solvent, washing solvent and the volume of them. After a serious of experiments, the optimum conditions were as follows: the volume of elution solvent was 4 mL of methanol and the washing solvent was acetonitrile-water 2:8(v/v). The calibration curve for ZEA was linear in the range of 10.00-300.00 μg/kg. The limit of detection and limit of quantitative was 2.00 μg/kg and 6.65 μg/kg, respectively. This method could provide a good reusability of 8 times and enough recoveries at three spiked levels (3, 5 and 8 ng/mL) ranging between 61.97% and 95.15% with the relative standard deviations of 1.94%∼7.44%. These results demonstrated that [email protected] could be used for separation, concentration and detection of ZEA from real samples.
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http://dx.doi.org/10.1016/j.jchromb.2022.123297DOI Listing
May 2022

[Detection of Porphyromonas gingivalis rag genotypes in patients of chronic periodontitis with chronic obstructive pulmonary disease].

Shanghai Kou Qiang Yi Xue 2022 Feb;31(1):100-103

Department of Stomatology, Weifang People's Hospital. Weifang 261000, Shandong Province, China. E-mail:

Purpose: To investigate the distribution of Porphyromonas gingivalis(P.g) rag genotypes in patients of chronic periodontitis with chronic obstructive pulmonary disease (COPD).

Methods: Thirty patients with chronic periodontitis and 30 patients with chronic periodontitis complicated with COPD were included. Saliva samples were collected from all subjects. The detection rate and rag genotype of P.g in saliva were detected by 16S rDNA polymerase chain reaction (PCR). SPSS 22.0 software package was used for statistical analysis.

Results: The positive rate of P.g was 76.67% in chronic periodontitis patients with COPD, and 63.33% in chronic periodontitis group, there was no significant difference between the two groups (P>0.05). The detection rates of rag-1 genotype in the two groups were 70% and 30.77%, respectively, there was significant difference between the two groups(P<0.05). The detection rates of rag-2, rag-3 and rag-4 in the two groups were not significantly different.

Conclusions: Various rag genotypes can be found in patients of chronic periodontitis with COPD. Rag-1 might have more close correlation with the development of COPD.
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February 2022

Late-Onset Acute Kidney Injury is a Poor Prognostic Sign for Severe Burn Patients.

Front Surg 2022 2;9:842999. Epub 2022 May 2.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Background: Acute kidney injury (AKI) is a morbid complication and the main cause of multiple organ failure and death in severely burned patients. The objective of this study was to explore epidemiology, risk factors, and outcomes of AKI for severely burned patients.

Methods: This retrospective study was performed with prospectively collected data of severely burned patients from the Institute of Burn Research in Southwest Hospital during 2011-2017. AKI was diagnosed according to Kidney Disease Improving Global Outcomes (KDIGO) criteria (2012), and it was divided into early and late AKIs depending on its onset time (within the first 3 days or >3 days post burn). The baseline characteristics, clinical data, and outcomes of the three groups (early AKI, late AKI and non-AKI) were compared using logistic regression analysis. Mortality predictors of patients with AKI were assessed.

Results: A total of 637 adult patients were included in analysis. The incidence of AKI was 36.9% (early AKI 29.4%, late AKI 10.0%). Multiple logistic regression analysis revealed that age, gender, total burn surface area (TBSA), full-thickness burns of TBSA, chronic comorbidities (hypertension or/and diabetes), hypovolemic shock of early burn, and tracheotomy were independent risk factors for both early and late AKIs. However, sepsis was only an independent risk factor for late AKI. Decompression escharotomy was a protective factor for both AKIs. The mortality of patients with AKI was 32.3% (early AKI 25.7%, late AKI 56.3%), and that of patients without AKI was 2.5%. AKI was independently associated with obviously increased mortality of severely burned patients [early AKI, OR = 12.98 (6.08-27.72); late AKI, OR = 34.02 (15.69-73.75)]. Compared with patients with early AKI, patients with late AKI had higher 28-day mortality (34.9% vs. 19.4%,  = 0.007), 90-day mortality (57.1% vs. 27.4%,  < 0.0001).

Conclusions: AKI remains prevalent and is associated with high mortality in severely burned patients. Late-onset acute kidney injury had greater severity and worse prognosis.
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http://dx.doi.org/10.3389/fsurg.2022.842999DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9108380PMC
May 2022

Association of acute kidney disease with the prognosis of ischemic stroke in the Third China National Stroke Registry.

BMC Nephrol 2022 May 18;23(1):188. Epub 2022 May 18.

China National Clinical Research Center for Neurological Diseases, Beijing, China.

Background: Acute kidney disease (AKD) evolves a spectrum of acute and subacute kidney disease requiring a global strategy to address. The present study aimed to explore the impact of AKD on the prognosis of ischemic stroke.

Methods: The Third China National Stroke Registry (CNSR-III) was a nationwide registry of ischemic stroke or transient ischemic attack between August 2015 and March 2018. As a subgroup of CNSR-III, the patients who had serum creatinine (sCr) and serum cystatin C (sCysC) centrally tested on admission and at 3-month, and with 1-year follow-up data were enrolled. Modified AKD criteria were applied to identify patients with AKD during the first 3 months post stroke according to the guidelines developed by the Kidney Disease: Improving Global Outcomes in 2012. The primary clinical outcome was 1-year all-cause death, and secondary outcomes were stroke recurrence and post stroke disability.

Results: Five thousand sixty-five patients were recruited in the study. AKD was identified in 3.9%, 6.7%, 9.9% and 6.2% of the patients by using sCr, sCr-based estimated glomerular filtration rate (eGFR), sCysC-based eGFR (eGFR), and combined sCr and sCysC-based eGFR (eGFR) criteria, respectively. AKD defined as sCr or eGFR criteria significantly increased the risk of all-cause mortality (adjusted HR 2.67, 95% CI: 1.27-5.61; adjusted HR 2.19, 95% CI: 1.17-4.10) and post stroke disability (adjusted OR 1.60, 95% CI: 1.04-2.44; adjusted OR 1.51, 95% CI: 1.08-2.11). AKD diagnosed by eGFR or eGFR criteria had no significant impact on the risk of all-cause death and post stroke disability. AKD, defined by whichever criteria, was not associated with the risk of stroke recurrence in the adjusted model.

Conclusions: AKD, diagnosed by sCr or eGFR criteria, were independently associated with 1-year all-cause death and post stroke disability in Chinese ischemic stroke patients.
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http://dx.doi.org/10.1186/s12882-022-02817-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115968PMC
May 2022

Nucleoporin37 may play a role in early embryo development in human and mice.

Mol Hum Reprod 2022 May 18. Epub 2022 May 18.

State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, 210029, China, Nanjing, Jiangsu Province.

Maternal-effect genes (MEGs) play an important role in maintaining the survival and development of mammalian embryos at the cleavage stage after fertilization. Despite long-term efforts, the MEGs that regulate preimplantation embryo development remain largely unknown. Here, using Whole-exome sequencing (WES) and homozygosity mapping, we identified a potential candidate gene associated with early embryo development: nucleoporin37 (NUP37), a nucleoporin gene that encodes a member of the nuclear pore complexes (NPCs) and regulates nuclear pore permeability and nucleocytoplasmic transport. Moreover, we determined the temporal and spatial expression patterns of Nup37 in mouse oocytes and early embryos, and explored the role of NUP37 in oocyte maturation and preimplantation embryo development. Immunoprecipitation assays confirmed that YAP1 binds to TEAD4 and NUP37. Furthermore, Nup37 gene knockdown reduced the nuclear import of YAP1 and downregulated the expression of YAP1-TEAD pathway downstream genes Rrm2 and Rpl13 in early embryos. Our study provides evidence that maternal NUP37 contributes to the nuclear import of YAP1 and then activates the YAP1-TEAD pathway, a signaling pathway essential for zygotic genome activation (ZGA). Nup37 may be a key gene involved in preimplantation embryo development in mammals.
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http://dx.doi.org/10.1093/molehr/gaac017DOI Listing
May 2022
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