Publications by authors named "Jinfeng Qiu"

7 Publications

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Preliminary Investigation about the Expression of G Protein-Coupled Receptors in Platelets from Patients with Chronic Immune Thrombocytopenic Purpura.

Acta Haematol 2021 Apr 13:1-9. Epub 2021 Apr 13.

Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Shantou University Medical College, Shantou, China.

Objective: The objective of this study was to determine the expression of G protein-coupled receptors (GPCRs) in platelets from adult patients with chronic immune thrombocytopenic purpura (ITP).

Methods: Peripheral blood samples were collected from 40 patients with chronic ITP in the Second Affiliated Hospital of Shantou University Medical College, and 40 peripheral blood samples from healthy volunteers were collected; expressions of the adenosine diphosphate receptors (P2Y1 and P2Y12), alpha-2A adrenergic receptor (α2A-AR), and thromboxane A2 receptor (TP) in platelets were detected by flow cytometry. Gα protein, protease-activated receptor 1 (PAR1), and protease-activated receptor 4 (PAR4) were analyzed by Western blot and analyzed statistically.

Results: Flow cytometry measurements of mean fluorescence intensities showed platelets from patients with chronic ITP, compared to healthy individuals, had significantly higher levels of P2Y1 (31.4 ± 2.2 vs. 7.8 ± 0.8), P2Y12 (29.6 ± 2.1 vs. 7.2 ± 1.3), α2A-AR (25.8 ± 2.9 vs. 9.8 ± 0.9), and TP (39.8 ± 3.1 vs. 4.7 ± 0.6) (all p < 0.01). Similarly, integrated optical density analysis of Western blots showed that platelets from patients with chronic ITP had significantly higher levels of Gα (1046.3 ± 159.96 vs. 254.49 ± 39.51), PAR1 (832.98 ± 98.81 vs. 203.92 ± 27.47), and PAR4 (1518.80 ± 272.45 vs. 431.27 ± 41.86) (all p < 0.01).

Conclusion: Expression of GPCRs is increased in platelets from patients with chronic ITP, suggesting that platelets of chronic ITP may participate in the complicated biological process by means of GPCR-mediated signaling pathways.
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http://dx.doi.org/10.1159/000514907DOI Listing
April 2021

Extremely rare case of intravascular solitary fibrous tumour in the inferior vena cava with review of the literature.

Diagn Pathol 2019 Aug 7;14(1):86. Epub 2019 Aug 7.

Department of Orthopedics and Traumatology Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, People's Republic of China.

Background: Solitary fibrous tumour (SFT) is a mesenchymal tumour of fibroblastic type, and it develops in almost any part of the human body. However, according to previous studies, the occurrence of intravascular SFTs is extremely rare.

Case Presentation: We reported a case of intravascular SFT in a 67-year-old woman who has been experiencing swelling and pain in the right leg for 2 months. Computed tomography venography scan revealed a well-defined mass obstructing the inferior vena cava (IVC). Surgical resection was performed, and histopathologic and immunohistochemical results were consistent with SFT. Further, next-generation sequencing (NGS) analysis was performed, and results revealed two tumour-related gene mutations (deletion of PMS2 and variation of ESR1 [L536P]). The patient did not receive any adjuvant therapy, and no signs of tumour progression were observed during the 6-month follow-up.

Conclusion: To the best of our knowledge, this study first presented about SFT arising from the IVC and carried out an NGS analysis to validate the molecular mechanism of such condition.
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http://dx.doi.org/10.1186/s13000-019-0862-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6686241PMC
August 2019

Multiple foci modulation with controllable positions and intensity ratios through decomposed optimization.

Opt Lett 2019 May;44(9):2354-2357

In this Letter, multiple foci modulation with controllable positions and intensity ratios is presented with a multi-belt binary phase mask in a tightly focusing system. Different from previous methods, the diffractive optical element (DOE) in our model is first virtually decomposed into two or more simple sub-DOEs. Then, after optimization, the sub-DOEs are combined to form the desired DOE through superposition. By such a decomposed optimization, the optimization complexities are greatly reduced, and the calculation becomes simpler and more effective. Furthermore, since the quantities and structures of sub-DOEs can be adjusted according to the original DOE, the method is very flexible and adaptable. As a demonstration, up to nine foci on the optical axis are studied, and the simulation results show that multiple foci can be well modulated. The proposed method may provide a universal strategy for complex light field modulations in a simple way.
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http://dx.doi.org/10.1364/OL.44.002354DOI Listing
May 2019

Fabrication of high fill factor cylindrical microlens array with isolated thermal reflow.

Appl Opt 2018 Sep;57(25):7296-7302

We demonstrate a simple, controllable, and stable method for fabricating high fill factor cylindrical microlens array with a novel isolated thermal reflow process. In this method, microstripes with a very small gap were obtained via digital micromirror device-based lithography, then covered with polydimethylsiloxane (PDMS) solution. The prepared microstripes were isolated and were heated and reflowed to a cylindrical microlens array. During the reflow process, the semicross-linked PDMS can serve as a barrier to prevent the diameter change and the bonding of adjacent microlenses. By this special treatment, the fill factor of the cylindrical microlens array can be significantly improved. Moreover, the reflow time and temperature have very little effect on the microlens shape due to the surrounded semicross-linked PDMS. This will make our process stabler than traditional methods. The measured 3D profile is good and satisfactory, and excellent optical performance is demonstrated with the fabricated cylindrical microlens arrays. The proposed method may offer a viable route for fabrication of high fill factor microlens arrays in a very simple and stable way.
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http://dx.doi.org/10.1364/AO.57.007296DOI Listing
September 2018

Treating acute cystitis with biodegradable micelle-encapsulated quercetin.

Int J Nanomedicine 2012 8;7:2239-47. Epub 2012 May 8.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Chengdu, People's Republic of China.

Intravesical application of an anti-inflammatory drug is an efficient strategy for acute cystitis therapy. Quercetin (QU) is a potent anti-inflammatory agent; however, its poor water solubility restricts its clinical application. In an attempt to improve water solubility of QU, biodegradable monomethoxy poly(ethylene glycol)-poly(ɛ-caprolactone) (MPEG-PCL) micelles were used to encapsulate QU by self-assembly methods, creating QU/MPEG-PCL micelles. These QU/MPEG-PCL micelles with DL of 7% had a mean particle size of <34 nm, and could release QU for an extended period in vitro. The in vivo study indicated that intravesical application of MPEG-PCL micelles did not induce any toxicity to the bladder, and could efficiently deliver cargo to the bladder. Moreover, the therapeutic efficiency of intravesical administration of QU/MPEG-PCL micelles on acute cystitis was evaluated in vivo. Results indicated that QU/MPEG-PCL micelle treatment efficiently reduced the edema and inflammatory cell infiltration of the bladder in an Escherichia coli-induced acute cystitis model. These data suggested that MPEG-PCL micelle was a candidate intravesical drug carrier, and QU/MPEG-PCL micelles may have potential application in acute cystitis therapy.
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http://dx.doi.org/10.2147/IJN.S29416DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357976PMC
December 2012

Gene therapy for C-26 colon cancer using heparin-polyethyleneimine nanoparticle-mediated survivin T34A.

Int J Nanomedicine 2011 19;6:2419-27. Epub 2011 Oct 19.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Chengdu, People's Republic of China.

Background: Gene therapy provides a novel method for the prevention and treatment of cancer, but the clinical application of gene therapy is restricted, mainly because of the absence of an efficient and safe gene delivery system. Recently, we developed a novel nonviral gene carrier, ie, heparin-polyethyleneimine (HPEI) nanoparticles for this purpose.

Methods And Results: HPEI nanoparticles were used to deliver plasmid-expressing mouse survivin-T34A (ms-T34A) to treat C-26 carcinoma in vitro and in vivo. According to the in vitro studies, HPEI nanoparticles could efficiently transfect the pGFP report gene into C-26 cells, with a transfection efficiency of 30.5% ± 2%. Moreover, HPEI nanoparticle-mediated ms-T34A could efficiently inhibit the proliferation of C-26 cells by induction of apoptosis in vitro. Based on the in vivo studies, HPEI nanoparticles could transfect the Lac-Z report gene into C-26 cells in vivo. Intratumoral injection of HPEI nanoparticle-mediated ms-T34A significantly inhibited growth of subcutaneous C-26 carcinoma in vivo by induction of apoptosis and inhibition of angiogenesis.

Conclusion: This research suggests that HPEI nanoparticle-mediated ms-T34A may have a promising role in C-26 colon carcinoma therapy.
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http://dx.doi.org/10.2147/IJN.S23582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3205136PMC
May 2012

Marked enhancement of photocatalytic activity and photochemical stability of N-doped TiO2 nanocrystals by Fe3+/Fe2+ surface modification.

J Colloid Interface Sci 2010 Mar 10;343(1):200-8. Epub 2009 Nov 10.

Department of Environmental Engineering, Zhejiang University, Hangzhou 310027, PR China.

N-doped TiO(2) (N-TiO(2)) nanocrystals with anatase and rutile mixed phases were prepared by partial oxidation of TiN. The samples were further modified by Fe-ions through incipient wetness impregnation method. The as-prepared samples were characterized by XRD, TEM, XPS, Raman, EPR, UV-vis DRS, and PL in detail. The results indicated that Fe mainly existed as Fe(3+)/Fe(2+) ions on the catalyst surface. The addition of small amounts of Fe-ions to N-TiO(2) nanocrystals caused several times enhancement of the photocatalytic activity under visible, UV and UV-vis light irradiation in degradation of gaseous toluene. The optimized Fe-ions content in this investigation was 0.02 wt.%. EPR and PL clearly showed that Fe(3+)/Fe(2+) redox cycle facilitated electron/hole charge separation, and contributed to the enhanced photocatalytic performance. Moreover, the photochemical stability of N-TiO(2) nanocrystals under visible light was improved due to the stabilization of nitrogen atoms in TiO(2) lattice by surface Fe-ions modification. The N-doped TiO(2) nanocrystals without Fe-ions modification suffered from a gradual deactivation due mainly to the loss of lattice-nitrogen during the photocatalytic reaction. The way to modification of nonmetal-doped TiO(2) nanomaterials brought new concept in enhancing the photocatalytic performance from the viewpoint of practical application.
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http://dx.doi.org/10.1016/j.jcis.2009.11.012DOI Listing
March 2010