Publications by authors named "Jinfeng Ding"

41 Publications

Methods for microplastic sampling and analysis in the seawater and fresh water environment.

Methods Enzymol 2021 15;648:27-45. Epub 2021 Jan 15.

Key Laboratory of Marine Chemistry Theory and Technology (Ocean University of China), Ministry of Education, Qingdao, China.

Microplastic (plastic smaller than 5mm in size) is ubiquitous around the world both in the ocean and the freshwater system. Due to their potential serious negative impact on marine organisms and human beings, marine microplastics have attracted worldwide attention in the past decade. Information and knowledge of the spatial and temporal distribution of marine microplastics are crucial for accurately assessing our current and future environmental health conditions. This is also important for developing mitigation plans and measures to protect our environment. Since the measured microplastic pollution level is closely related to the sampling methods and identification techniques, it is important to employ standardized sampling and analysis operation procedures for cross-comparison. In this chapter, we present the basic sampling, sample pretreatment and microplastic identification techniques involved in microplastic pollution assessment and discuss the adaptability of different sampling and pretreatment methods. The pros and cons of different techniques are also discussed.
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http://dx.doi.org/10.1016/bs.mie.2020.12.009DOI Listing
January 2021

Tillage and seeding strategies for wheat optimizing production in harvested rice fields with high soil moisture.

Sci Rep 2021 Jan 8;11(1):119. Epub 2021 Jan 8.

Jiangsu Key Laboratory of Crop Genetics and Physiology, and Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, 225009, Jiangsu, China.

Suitable tillage and seeding strategies for wheat can be used to combat excessive residues and poor soil conditions in harvested rice fields. This study investigated the effects of different tillage (zero tillage and rotary tillage) and seeding methods on wheat growth, grain yield, nitrogen (N) uptake and utilization, and economic benefit when the soil moisture was high during the tillage and seeding practices. In 2016-2017, three seeders were tested: SM1-1, SM2, and SM3; in 2017-2018, four seeders were tested: SM1-2, SM2, SM3, and SM4. Although the soil moisture was different between years, zero tillage could be used to reduce the sowing depth, which facilitated early-phase wheat growth and N uptake compared with rotary tillage, resulting in higher grain yield, NUpE, and net return. In 2016-2017 (high wet soil), a small-size seeder (SM1-1) with sowing near the soil surface facilitated higher grain yield, NUpE, and net returns compared with the other seeders; in 2017-2018 (low wet soil), medium-size seeders (SM3 and SM4) were more suitable than small-size seeders (SM1-2 and SM2). In both years, the seeders that performed the best mainly improved the spike numbers while increasing N uptake, especially after anthesis. Zero tillage lowered input costs, but small-size seeders did not reduce costs due to the higher labor costs associated with their low working efficiency. Improving net returns depends largely on increasing yield. In conclusion, zero tillage is recommended for wheat production in harvested rice fields with a high soil moisture content, but the suitable seeding method needs to be confirmed according to the soil moisture content.
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http://dx.doi.org/10.1038/s41598-020-80256-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794509PMC
January 2021

The Chinese National Stem Cell Resource Center.

Stem Cell Res 2020 Sep 17;50:101985. Epub 2020 Sep 17.

National Stem Cell Resource Centre, Institute of Zoology, Chinese Academy of Sciences, Beijing 100190, China; Innovation Academy for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China.

The Chinese National Stem Cell Resource Center was first established in 2007 and has progressed to produce and prepare stocks of more than 400 human embryonic stem cell lines. Its facilities are accredited to international standards and it has accreditation as a supplier of cells for research and therapy. The NSCRC also has an active program of translational research and strong collaborations with the Institute of Zoology and the Academy for Stem Cells and Regeneration of the Chinese Academy of Sciences. Its translational research extends to early stage clinical studies and it also has a strong training and public education program.
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http://dx.doi.org/10.1016/j.scr.2020.101985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494438PMC
September 2020

The Analgesic Efficacy of Erector Spinae Plane Blocks in Patients Undergoing Posterior Lumbar Spinal Surgery for Lumbar Fracture.

World Neurosurg 2020 Dec 16. Epub 2020 Dec 16.

Department of Anesthesiology, Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University, Linhai, China. Electronic address:

Objective: To investigate the postoperative analgesic efficacy of ultrasound-guided lumbar erector spinae plane (ESP) blocks in patients undergoing posterior lumbar spinal surgery for lumbar spinal fractures.

Methods: A total of 80 patients who were scheduled for posterior internal fixation for lumbar spinal fractures were divided into a patient-controlled analgesia (PCA) group or a combined ESP-PCA group. Numeric rating scale at rest and during movement, postoperative sufentanil consumption, and accumulative and effective bolus presses of PCA were recorded at 6, 12, 24, and 48 hours postoperatively. Numeric rating scale at rest and during movement was the primary outcome. Incidence of postoperative nausea and vomiting during the first 24-48 hours, pruritus and chronic postoperative pain, and dose of pethidine for rescue analgesia were also recorded.

Results: Numeric rating scale at rest and during movement at 6, 12, and 24 hours was lower in the ESP-PCA group (P < 0.001, P < 0.001, P = 0.0016 at rest; all P < 0.001 during movement). Lumbar ESP blocks diminished accumulative bolus presses and effective bolus presses of PCA at 6, 12, 24, and 48 hours postoperatively. Besides, patients in the ESP-PCA group had fewer demands for sufentanil and pethidine. The incidence of postoperative nausea and vomiting in the ESP-PCA group was lower than that in PCA group.

Conclusions: PCA combined with lumbar ESP blocks provided superior postoperative analgesia for patients with lumbar spinal fractures treated with posterior internal fixation. Lumbar ESP blocks decreased postoperative opioid consumption and incidence of postoperative nausea and vomiting, thereby enhancing postoperative recovery.
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http://dx.doi.org/10.1016/j.wneu.2020.10.175DOI Listing
December 2020

Palliative care needs and utilisation of different specialist services in the last days of life for people with lung cancer.

Eur J Cancer Care (Engl) 2021 Jan 27;30(1):e13331. Epub 2020 Oct 27.

School of Population and Global Health, The University of Western Australia, Perth, WA, Australia.

Objectives: To (a) compare palliative care needs of lung cancer patients on their final admission to community-based and inpatient palliative care services; and (b) explore whether and how these care needs affect their utilisation of different palliative care services in the last days of life.

Methods: Descriptive study involving 17,816 lung cancer patients who received the last episode of palliative care from specialist services and died between 1 January 2013 and 31 December 2018.

Results: Both groups of patients admitted to community-based and inpatient palliative care services generally experienced relatively low levels of symptom distress, but high levels of functional impairment and dependency. "Unstable" versus "stable" palliative care phase (Odds ratio = 11.66; 95% Confidence Interval: 9.55-14.24), poorer functional outcomes and severe levels of distress from many symptoms predicted greater likelihood of use of inpatient versus community-based palliative care.

Conclusions: Most inpatient palliative care admissions are not associated with high levels of symptom severity. To extend the period of home care and rate of home death for people with lung cancer, additional investment is required to improve their access to sufficiently skilled palliative care staff, multi-disciplinary teams and 24-hour home support in community settings.
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http://dx.doi.org/10.1111/ecc.13331DOI Listing
January 2021

Requirements for human embryonic stem cells.

Cell Prolif 2020 Dec 19;53(12):e12925. Epub 2020 Oct 19.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute for Stem Cell and Regeneration, Institute of Zoology, National Stem Cell Resource Center, Chinese Academy of Sciences, Beijing, China.

'Requirements for Human Embryonic Stem Cells' is the first set of guidelines on human embryonic stem cells in China, jointly drafted and agreed upon by experts from the Chinese Society for Stem Cell Research. This standard specifies the technical requirements, test methods, test regulations, instructions for use, labelling requirements, packaging requirements, storage requirements and transportation requirements for human embryonic stem cells, which is applicable to the quality control for human embryonic stem cells. It was originally released by the China Society for Cell Biology on 26 February 2019 and was further revised on 30 April 2020. We hope that publication of these guidelines will promote institutional establishment, acceptance and execution of proper protocols, and accelerate the international standardization of human embryonic stem cells for applications.
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http://dx.doi.org/10.1111/cpr.12925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705896PMC
December 2020

New insights into the toxic interactions of polyvinyl chloride microplastics with bovine serum albumin.

Environ Sci Pollut Res Int 2021 Feb 23;28(5):5520-5531. Epub 2020 Sep 23.

Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, 6 Xianxialing Road, Qingdao, 266061, People's Republic of China.

The binding interaction between emerging pollutant polyvinyl chloride microplastics (PVC MPs) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy, resonance scattering spectroscopy (RLS), UV-visible (UV-vis) absorption spectroscopy, circular dichroism (CD), and Fourier transform infrared (FT-IR) spectroscopy under simulative physiological conditions. Fluorescence results revealed that the fluorescence quenching of BSA induced by PVC MPs was originated from the formation of BSA-PVC complex in static quenching mode. According to Stern-Volmer equation, the binding constants (K) between PVC MPs and BSA at different temperatures were obtained, and the number of binding sites was 1.62. The thermodynamic parameters, enthalpy change (ΔH), entropy change (ΔS), and free energy change (ΔG) were calculated to be - 41.77 kJ mol, 43.17 J mol K, and - 54.63 kJ mol via Van't Hoff equation, indicating electrostatic interaction played a key role in the formation of BSA-PVC complex spontaneously. In addition, the alterations of microenvironment and secondary structure in BSA induced by PVC MPs were further confirmed by synchronous fluorescence spectra, UV-vis, FT-IR, and CD. This work not only provides further information for better understanding the binding interaction of PVC MPs with BSA, but also elucidates the potential biological toxicity of MPs at a molecular level.
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http://dx.doi.org/10.1007/s11356-020-10707-1DOI Listing
February 2021

An examination of the occurrence and potential risks of microplastics across various shellfish.

Sci Total Environ 2020 Oct 8;739:139887. Epub 2020 Jun 8.

Menaul School, Qingdao 266071, China.

The ingestion of microplastics by shellfish pose a potential health risk for human via seafood consumption. This study investigated and compared the contamination levels and potential human health risks of microplastics in the digestive system of commercial shellfish from North (Qingdao) and South (Xiamen) China. Microplastics were detected in 70%-100% of shellfish samples from Qingdao and 70%-90% of shellfish samples from Xiamen, with abundances ranging from 1.2 to 4.1 items/individual (or 0.8-4.4 items/g, wet weight of digestive system) in shellfish from Qingdao and 1.3-6.0 items/individual (or 2.1-4.0 items/g) in shellfish from Xiamen. The microplastic composition was dominated by rayon and tended to be fibrous in shape, and white, black, and transparent in color. Microplastics <500 μm were the dominant size range, in which the size range of 100-200 μm was the most abundant size. Features of microplastics in the water-dwelling shellfish were different from those of the sediment-dwelling shellfish, and the microplastic features in the shellfish correlated with the sampling region, shellfish length, total wet body weight, and wet weight of the digestive system. Risk assessment results revealed that the potential human health risk posed by microplastics from the digestive system of commercial shellfish was higher in Qingdao than Xiamen.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139887DOI Listing
October 2020

Comparison of Transcriptome Profiles of the Fungus and Insect Pest in Response to Benzothiazole.

Front Microbiol 2020 12;11:1043. Epub 2020 Jun 12.

Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an, China.

Benzothiazole (BT) has a strong inhibitory effect on the growth and development of a wide spectrum of fungi and insects, such as and , that cause serious losses in agriculture. To investigate the underlying antifungal and insecticidal mechanisms of BT, RNA-seq analysis was performed for after BT treatment for 12, 24, and 48 h and for after BT treatment for 6 and 24 h. In , the pectin degradation process was inhibited, suggesting a low utilization of carbohydrate sources. As the treatment time was extended, the cell walls of thickened, and increases in melanin synthesis and ion transport were observed. In , signaling pathways including MAPK, insulin, adipocytokine, forkhead box class O, and peroxisome proliferator-activated receptor were activated at 6 h, and phosphoenolpyruvate carboxykinase was the core gene in the signal transduction pathways that responded to BT; digestive system and melanogenesis genes were obviously altered at 24 h. In addition, we identified several insecticidal target genes, such as trypsin, aminopeptidase N, and tyrosinase. Benzothiazole significantly affected nutrient metabolism, especially carbohydrate metabolism, in both species, and the pentose and glucuronate interconversions pathway was shared by both species, although the individual genes were different in each species. Overall, our results suggested that BT was a melanogenesis disrupter for the insect but an activator for the fungus. Our findings are helpful for deeply exploring the genes targeted by BT and for developing new pesticide compounds with unique mechanisms of action.
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http://dx.doi.org/10.3389/fmicb.2020.01043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325989PMC
June 2020

Palliative care needs and utilization of specialist services for people imminently dying with dementia: A national population-based study.

Int J Nurs Stud 2020 Sep 1;109:103655. Epub 2020 Jun 1.

Monash Nursing and Midwifery, Monash University, Clayton Campus, Level 1, 10 Chancellors Walk, Wellington Road, Clayton, VIC 3800, Australia; Supportive and Palliative Care, Eastern Health, 251 Mountain Highway, Wantirna, VIC, 3152, Australia; Australian Health Services Research Institute, Building 234 (iC Enterprise 1) Innovation Campus, University of Wollongong, Wollongong NSW 2522, Australia; Faculty of Health and Medical Sciences, The University of Western Australia, 35 Stirling Highway, Perth, WA 6009 Australia. Electronic address:

Background: There is a growing emphasis on the importance of availability of specialist palliative care for people living with dementia. However, for people imminently dying with dementia, we still have little knowledge about their palliative care needs and utilization of different specialist services.

Objectives: To (i) assess palliative care needs and other clinical and social characteristics of people imminently dying with dementia on their last admission in the context of community and inpatient palliative care services before death; (ii) compare care needs between patients requiring community-based and inpatient services; (iii) determine how and whether such care needs affect utilization of different palliative care services.

Design: Observational study using data from the Australian Palliative Care Outcomes Collaboration.

Settings: Specialist palliative care services across Australia registered in the Palliative Care Outcomes Collaboration.

Participants: A total of 3361 people who required specialist palliative care principally for dementia (including Alzheimer's disease and other dementias), and whose death occurred between 1 January 2013 and 31 December 2018.

Methods: Five validated clinical instruments were used to collect point-of-care outcomes on each individual's function (Resource Utilisation Groups - Activities of Daily Living & Australia-modified Karnofsky Performance Status), symptom distress (Symptoms Assessment Scale & Palliative Care Problem Severity Score) and other clinical characteristics (Palliative Care Phases). We fitted multivariate logistic regression models to examine the association between these clinical outcomes and utilization of different specialist palliative care services.

Results: The majority of people imminently dying with dementia had absent or mild levels of symptom distress but experienced high levels of functional decline and needed substantial assistance with basic tasks of daily living in their last days of life. Large disparities in symptoms distress and functional decline between inpatient and community groups were not observed although differences in assessment scores were often statistically significant. Poor functional outcomes (odds ratio = 1.77, 95% confidence interval: 1.24-2.52) and "non-stable" palliative care phases (odds ratio =24.51, 95% confidence interval: 12.03-49.96) were positively associated with use of inpatient versus community palliative care, whereas there was no clear association between the majority of symptoms and use of different care services.

Conclusions: The majority of people imminently dying with dementia could potentially benefit from greater access to supportive services in the community. Development of a dementia-specific palliative care pathway is needed to promote needs-based palliative care delivery models. TWEETABLE ABSTRACT: the majority of people imminently dying with dementia could potentially benefit from greater access to supportive services in community.
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http://dx.doi.org/10.1016/j.ijnurstu.2020.103655DOI Listing
September 2020

Patient-Reported Barriers and Facilitators to Seeking and Accessing Support in Gynecologic and Breast Cancer Survivors With Sexual Problems: A Systematic Review of Qualitative and Quantitative Studies.

J Sex Med 2020 07 21;17(7):1326-1358. Epub 2020 Apr 21.

Monash Nursing and Midwifery, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, VIC, Australia; Supportive and Palliative Care, Eastern Health, Melbourne, VIC, Australia. Electronic address:

Background: Greater understanding of patient-reported barriers and facilitators to seeking and accessing sexual health services will help formulate strategies to assist gynecologic and breast cancer patients to overcome obstacles to accessing sexual health support because they typically do not seek sexual education and/or treatment when confronted with sexual concerns.

Aim: The objectives of this systematic review were to (i) explore the patient-reported barriers to seeking and accessing support for sexual problems in gynecologic and breast cancer survivors, and (ii) identify strategies used to successfully overcome the barriers to accessing sexual health information and/or treatment.

Main Outcome Measures: The main outcome measures included factors that prevent and/or facilitate gynecologic and breast cancer patients with sexual concerns seeking and accessing sexual health-related services.

Methods: Systematic searches of major electronic databases (Ovid MEDLINE, PsycINFO, CINAHL, ProQuest, and Chinese database CNKI) from January 2009 to July 2019 were used to identify the barriers and facilitators to seeking sexual education/treatment from the perspective of gynecologic and breast cancer survivors. A narrative synthesis was conducted.

Results: 20 studies met the inclusion criteria including 12 qualitative, 6 quantitative, and 2 mixed methods studies. 4 interconnected themes were derived from 13 subthemes relating to the barriers/facilitators to seeking and accessing sexual health support. The most common barriers were embarrassment/discomfort in discussing sexual concerns, perceived discomfort of healthcare providers in discussing sexual issues, limitations of the healthcare system to address sexual problems, and the multidimensional nature of sexuality. Help-seeking for sexual health concerns was facilitated by: (i) oncology health professionals initiating and conducting open, honest discussions around sexual concerns with patients; (ii) the availability of information in multiple forms; and (iii) appropriate timing of information provision according to women's preferences.

Clinical Implications: Oncology health professionals need to develop an open, honest, accepting communication style and be accessible to women with cancer and their partners within healthcare systems.

Strengths & Limitations: The systematic review was conducted in accordance with guidelines. Variability in the primary aims and outcomes of the included studies precluded a meta-analysis.

Conclusions: Training programs for providers of oncology care should enhance their knowledge of sexual issues in gynecologic and/or breast cancer, enhance their communication skills with patients, and improve their ability to consult or refer patients to psycho-oncologists or other mental health professionals. Dai Y, Cook OY, Yeganeh L, et al. Patient-Reported Barriers and Facilitators to Seeking and Accessing Support in Gynecologic and Breast Cancer Survivors With Sexual Problems: A Systematic Review of Qualitative and Quantitative Studies. J Sex Med 2020;17:1326-1358.
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http://dx.doi.org/10.1016/j.jsxm.2020.03.004DOI Listing
July 2020

Characteristics of People with Dementia vs Other Conditions on Admission to Inpatient Palliative Care.

J Am Geriatr Soc 2020 08 24;68(8):1825-1833. Epub 2020 Apr 24.

School of Population and Global Health, The University of Western Australia, Crawley, Australia.

Objectives: Our aim was to (1) describe the clinical characteristics and symptoms of people diagnosed with dementia at the time of admission to inpatient palliative care; and (2) compare the nature and severity of these palliative care-related problems to patients with other chronic diseases.

Design: Descriptive study using assessment data on point of care outcomes (January 1, 2013, to December 31, 2018).

Setting: A total of 129 inpatient palliative care services participating in the Australian Palliative Care Outcomes Collaboration.

Participants: A total of 29,971 patients with a primary diagnosis of dementia (n = 1,872), lung cancer (n = 19,499), cardiovascular disease (CVD, n = 5,079), stroke (n = 2,659), or motor neuron disease (MND, n = 862).

Measurements: This study reported the data collected at the time of admission to inpatient palliative care services including patients' self-rated levels of distress from seven common physical symptoms, clinician-rated symptom severity, functional dependency, and performance status. Other data analyzed included number of admissions, length of inpatient stay, and palliative care phases.

Results: At the time of admission to inpatient palliative care services, relative to patients with lung cancer, CVD, and MND, people with dementia presented with lower levels of distress from most symptoms (odds ratios [ORs] range from .15 to .80; P < .05 for all) but higher levels of functional impairment (ORs range from 3.02 to 8.62; P < .001 for all), and they needed more assistance with basic activities of daily living (ORs range from 3.83 to 12.24; P < .001 for all). The trends were mostly the opposite direction when compared with stroke patients. Patients with dementia tended to receive inpatient palliative care later than those with lung cancer and MND.

Conclusion: The unique pattern of palliative care problems experienced by people with dementia, as well as the skills of the relevant health services, need to be considered when deciding on the best location of care for each individual. Access to appropriately trained palliative care clinicians is important for people with high levels of physical or psychological concerns, irrespective of the care setting or diagnosis. J Am Geriatr Soc 68:1825-1833, 2020.
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http://dx.doi.org/10.1111/jgs.16458DOI Listing
August 2020

Structure and physicochemical properties of two waxy wheat starches.

Food Chem 2020 Jul 25;318:126492. Epub 2020 Feb 25.

Department of Grain Science and Industry, Kansas State University, Manhattan, KS 66506, USA. Electronic address:

The objective of this study was to determine the primary influences of amylopectin (AP) on starch properties using two waxy wheat starches with different pasting properties. Compared with Yang Nuo Mai 1 (YNM1) starch, Ning Nuo Mai 1 (NNM1) starch had a greater average granule diameter, hot water solubility, gelatinization enthalpy, higher pasting viscosity, but lower retrogradation enthalpy. NNM1 starch had a greater proportion of AP long chains, and lower proportion of AP short chains. Therefore, NNM1 starch had a greater degree of crystallinity as well as stronger resonance intensities at the C1, C2, C3, C5, and C6 by solid-state C CP/MAS NMR. These results suggested that the proportion of long AP chains (DP ≥ 37) is the main influencing factor in determining waxy starch physicochemical properties. In addition, the proportion of short AP chains (DP 13-24) plays a role in promoting retrogradation of waxy wheat starch.
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http://dx.doi.org/10.1016/j.foodchem.2020.126492DOI Listing
July 2020

End-of-life care in general practice: clinic-based data collection.

BMJ Support Palliat Care 2020 Feb 17. Epub 2020 Feb 17.

Medical School, The University of Western Australia, Perth, Western Australia, Australia.

Background: There are no processes that routinely assess end-of-life care in Australian general practice. This study aimed to develop a data collection process which could collect observational data on end-of-life care from Australian general practitioners (GPs) via a questionnaire and clinical data from general practice software.

Methods: The data collection process was developed based on a modified Delphi study, then pilot tested with GPs through online surveys across three Australian states and data extraction from general practice software, and finally evaluated through participant interviews.

Results: The developed data collection process consisted of three questionnaires: Basic Practice Descriptors (32 items), Clinical Data Query (32 items) and GP-completed Questionnaire (21 items). Data extraction from general practice software was performed for 97 decedents of 10 GPs and gathered data on prescriptions, investigations and referral patterns. Reports on care of 272 decedents were provided by 63 GPs. The GP-completed Questionnaire achieved a satisfactory level of validity and reliability. Our interviews with 23 participating GPs demonstrated the feasibility and acceptability of this data collection process in Australian general practice.

Conclusions: The data collection process developed and tested in this study is feasible and acceptable for Australian GPs, and comprehensively covers the major components of end-of-life care. Future studies could develop an automated data extraction tool to reduce the time and recall burden for GPs. These findings will help build a nationwide integrated information network for primary end-of-life care in Australia.
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http://dx.doi.org/10.1136/bmjspcare-2019-002006DOI Listing
February 2020

Current distribution characteristics of trace elements in the coral-reef systems of Xisha Islands, China.

Mar Pollut Bull 2020 Jan 30;150:110737. Epub 2019 Nov 30.

Sansha Trackline Institute of Coral Reef Environment Protection, Sansha 573199, China.

The distribution characteristics, correlation, and bioavailability of 14 trace elements in different fishes and corals collected from the coral-reef systems of the Xisha Islands, China were systematic studied for the first time. All detected elements were measured using inductively coupled plasma mass spectrometry after pretreatment by microwave digestion. The fishes had slight differences in element enrichment selectivity and bioavailability with average levels as follows: V, 0.100 mg·kg; Cr, 0.683 mg·kg; Mn, 1.178 mg·kg; Fe, 51.806 mg·kg; Co, 0.072 mg·kg; Ni, 1.286 mg·kg; Cu, 1.428 mg·kg; Zn, 10.384 mg·kg; As, 6.437 mg·kg; Se, 0.830 mg·kg; Mo, 0.101 mg·kg; Cd, 0.051 mg·kg; Pb, 0.308 mg·kg; and U, 0.008 mg·kg. Trace element contents in corals were higher than those in fishes. The distribution characteristics of trace elements in fishes and corals presented a significant correlation in North Reef and Yongle Atoll, thereby indicating an evident effect on living areas compared with trace elements in fishes from the Spratly Islands. Cluster analysis of fishes and corals based on trace elements showed that they had different selectivities and bioavailabilities to trace elements. The EDI and THQ ratios of Cu, As, Cd, Cr, and Pb demonstrated that fishes were unlikely to experience serious adverse effects, except for As in Chaetodon auriga, Myripristis kuntee, Gnathodentex aureolineatus, and Hemigymnus melapterus in Xisha Islands.
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http://dx.doi.org/10.1016/j.marpolbul.2019.110737DOI Listing
January 2020

Identification and Expression Analysis of Chemosensory Receptor Genes in Bradysia odoriphaga (Diptera: Sciaridae).

J Econ Entomol 2020 02;113(1):435-450

College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, P.R. China.

The chive midge, Bradysia odoriphaga, is a major insect pest affecting Chinese chive production in China. Its adult life stage is nonfeeding and has a short life span. Hence, the perception of chemical stimuli is important for its adult behavior and reproductive success. To better understand its chemosensory process at the molecular level, chemosensory receptor genes were identified based on transcriptomes of B. odoriphaga. In total, 101 chemosensory genes were identified from the antenna and body transcriptomes, including 71 odorant receptors (ORs), 18 ionotropic receptors (IRs), 5 gustatory receptors (GRs), and 7 sensory neuron membrane proteins (SNMPs). Phylogenetic analysis indicated that most of these genes have homologs among other Dipteran insects. A transcript abundance comparison based on FPKM values was conducted to analyze the sex- and tissue-specific expression profiles of these chemosensory genes. Moreover, quantitative real-time PCR of OR transcripts was performed on different tissues (female antennae, male antennae, heads, and legs) to verify the transcriptional expression levels of ORs in the transcriptomes. This analysis suggested that 44 ORs showed significantly higher expression in the female antennae, while 16 OR transcripts were most highly expressed in the male antennae and may play significant roles in sex pheromone detection. In addition, some IRs and GRs might be involved in CO2 and sugar detection and temperature sensing. In the present study, 101 chemosensory genes were identified, and their putative functions were predicted. This work could provide a basis to facilitate functional clarification of these chemosensory genes at the molecular level.
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http://dx.doi.org/10.1093/jee/toz286DOI Listing
February 2020

End-of-life care in rural general practice: how best to support commitment and meet challenges?

BMC Palliat Care 2019 Jun 25;18(1):51. Epub 2019 Jun 25.

School of Nursing and Midwifery, Monash University, Wellington Road, Clayton, Victoria, 3800, Australia.

Background: Few studies have specifically assessed the scope, nature and challenges of palliative and end-of-life care in rural general practice. These knowledge gaps limit the development of evidence-based policies and services for patients in the last months of life. This study aimed to explore the perspectives of general practitioners (GPs) and other stakeholders on rural GPs' involvement and challenges in providing palliative and end-of-life care in regional Australia.

Methods: A qualitative study involving five focus groups with 26 GPs based in rural/regional Western Australia together with 15 individual telephone interviews with four GPs and 11 other stakeholders involved in end-of-life care across Australia.

Results: The rural GPs' central role in end-of-life care was recognized by the majority of participants but multiple challenges were also identified. Some challenges were comparable to those found in urban settings but others were more pronounced, including resource limitations and lack of training. Inappropriate payment models discouraged GPs' involvement in some aspects of end-of-life care, such as case conferences and home visits. Compared to GPs in urban settings, those in rural/regional communities often reported closer doctor-patient relationships and better care integration and collaboration. These positive aspects of care could be further developed to enhance service provision. Our study highlighted the importance of regular interactions with other professionals and patients in providing end-of-life care, but many GPs and other stakeholders found such interactions more challenging than the more "technical" aspects of care.

Conclusions: Rural/regional GPs appear to be disproportionately affected by inappropriate payment models and limited resources, but may benefit from closer doctor-patient relationships and better care integration and collaboration relative to urban GPs. Systematic collection of empirical data on GP management at end-of-life is required to build on these strengths and address the challenges.
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http://dx.doi.org/10.1186/s12904-019-0435-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593492PMC
June 2019

Microplastics in the Coral Reef Systems from Xisha Islands of South China Sea.

Environ Sci Technol 2019 Jul 2;53(14):8036-8046. Epub 2019 Jul 2.

Key Laboratory of Marine Bioactive Substances , First Institute of Oceanography, Ministry of Natural Resources , Qingdao 266061 , China.

The impacts of microplastics on coral reefs are gaining attention due to findings that microplastics affect coral health. This work investigated the distribution and characteristics of microplastics in the seawater, fish, and corals in 3 atolls from the Xisha Islands of South China Sea. In the seawater samples, microplastics were detected in the outer reef slopes, reef flats, and lagoons with abundances ranging from 0.2 to 11.2, 1.0 to 12.2, and 1.0 to 45.2 items L, respectively. Microplastic abundance was 0-12.0 items individual (0-4.7 items g) in fish and 1.0-44.0 items individual(0.02-1.3 items g) in coral. The predominant shape and polymer of microplastics in seawater, fish, and coral were fibrous rayon and polyethylene terephthalate (PET). Microplastic sizes primarily ranged from 20-330 μm in both the seawater and fish, while there were relatively more 1-5 mm microplastics in the corals. The shape, size, color, and polymer type distribution patterns of microplastics in seawater more closely resembled those in fish gills than those in fish gastrointestinal tracts or coral samples. This study shows that microplastics are abundant in these coral reef systems and they are captured by fish or "trapped" by corals.
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http://dx.doi.org/10.1021/acs.est.9b01452DOI Listing
July 2019

Distribution characteristics of microplastics in the seawater and sediment: A case study in Jiaozhou Bay, China.

Sci Total Environ 2019 Jul 3;674:27-35. Epub 2019 Apr 3.

Marine Ecology Research Center, First Institute of Oceanology, Ministry of Natural Resources, Qingdao 266061, China; Laboratory of Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address:

As a newly identified form of pollutant in the ocean, microplastics are receiving more and more attention from researchers. In order to evaluate the potential impact of microplastics, it is important to have comprehensive knowledge of the current microplastic situation. This study analyzed the abundance, polymer type, size, shape and color of microplastics in the seawater and sediments of Jiaozhou Bay, China, a semi-enclosed bay highly affected by surrounding human activities. Qualitative and quantitative analysis of microplastics were carried out using stereo microscope and attenuated total reflection micro-Fourier transform infrared spectrometer (ATR-μ-FT-IR). The abundance of microplastics in the bay seawater samples ranged between 20 items/m and 120 items/m. The abundance of microplastics in sediment samples in the bay were between 7 items/kg d.w. and 25 items/kg d.w. The microplastics were mainly in black and blue colors with fiber being the dominant shape in all samples. All microplastics identified were smaller than 4 mm in this study. Polyethylene terephthalate (PET) was the main type of microplastics, followed by polypropylene (PP) and polyethylene (PE). Both the abundance and types of microplastics show positive correlations between the seawater and sediment samples. Microplastic polymer types in the bay showed close match to the polymer types in the estuaries. Relatively higher abundance of microplastics tended to be found in the area where there were residual currents and sediment transport trends. Our study showed riverine input is an important contributor to the microplastics in the bay and physical factors such as residual currents would greatly affect the aggregation of microplastics.
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http://dx.doi.org/10.1016/j.scitotenv.2019.04.008DOI Listing
July 2019

Complete Genome Sequence of an -Acyl Homoserine Lactone Producer, sp. Strain L-A4, Isolated from Rhizosphere of in a Coastal Wetland.

Microbiol Resour Announc 2019 Jan 31;8(5). Epub 2019 Jan 31.

Key Laboratory for Marine Bioactive Substances and Modern Analytical Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao, China.

The sp. strain L-A4 was isolated from the rhizosphere of in the Qinhaungdao coastal wetland in China. Here, we present the complete genome sequence of strain L-A4, which consists of a chromosome of 5,029,620 bp with a G+C content of 64.53% and 4,964 coding DNA sequences. This strain was the first detected to produce -acyl homoserine lactone (AHL) signals in a member of this genus.
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http://dx.doi.org/10.1128/MRA.01539-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357640PMC
January 2019

Intrinsic peroxidase-like activity of CuZnSn(SSe) nanocrystals, and their application to the colorimetric detection of HO.

Mikrochim Acta 2019 01 19;186(2):118. Epub 2019 Jan 19.

Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Qingdao, 266237, People's Republic of China.

Nanocrystals (NCs) of type CuZnSn(SSe) (CZTSSe) were prepared via a solvothermal approach. They are shown to be highly efficient peroxidase (POx) mimics for colorimetric detection of HO. By varying the molar ratio of S and Se during preparation, the NCs showed different crystal structures, morphologies, surface properties, and POx-like activities. Among them, the type CZTSSe-0.25 NCs exhibit the strongest POx-like activities towards the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of HO to generate a blue product. The enhanced activity is attributed to its more negative potential and larger specific surface of the NCs. Based on these findings, a rapid and ultrasensitive method was developed for the visual and colorimetric determination of HO. The method is selective, and the NCs are reusable and long-term stable. The detection limit of HO is 50 nM. Kinetic and active species trapping experiments were performed to elucidate the POx-like mechanism of the NCs. Graphical abstract Schematic presentation of the process of CuZnSn(SSe) nanocrystals catalyzing the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of HO to induce a typical blue color reaction, which can be applied in colorimetric detection of HO.
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http://dx.doi.org/10.1007/s00604-018-3185-8DOI Listing
January 2019

Thiamethoxam, Clothianidin, and Imidacloprid Seed Treatments Effectively Control Thrips on Corn Under Field Conditions.

J Insect Sci 2018 Nov 1;18(6). Epub 2018 Nov 1.

College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, China.

With the widespread adoption of no-tillage technology, outbreaks of thrips have caused serious damage to summer corn fields in China. Therefore, effective control of pest populations is often essential for cost-effective crop production. In this study, experiments were conducted in 2014 and 2015 to determine the control efficacy of seven neonicotinoid insecticide seed treatments against corn thrips and the effects of these treatments on natural enemy population densities and emergence rates, seedling characteristics, and yield of corn. The results showed that among the tested neonicotinoid seed treatments, thiamethoxam (1.0 and 2.0 g active ingredient (AI)/kg of seeds), clothianidin (1.0 and 2.0 g AI/kg of seeds), and imidacloprid (2.0 g AI/kg of seeds) showed the highest control efficacy against corn thrips throughout the corn growing season. Seed treatments with acetamiprid, nitenpyram, dinotefuran, and thiacloprid at rates of 1.0 and 2.0 g AI/kg of seeds were difficult to effectively control thrips on summer corn. Neonicotinoid seed treatments showed no adverse effects on the numbers of spiders and lady beetles. Furthermore, treatments did not negatively influence the seedling growth or development of corn but did prevent yield losses. Therefore, treating corn seeds with thiamethoxam, clothianidin, and imidacloprid can provide effective protection against early-season thrips and reduce yield losses under field conditions.
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http://dx.doi.org/10.1093/jisesa/iey128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299462PMC
November 2018

Sublethal and Hormesis Effects of Clothianidin on the Black Cutworm (Lepidoptera: Noctuidae).

J Econ Entomol 2018 12;111(6):2809-2816

College of Plant Protection, Shandong Agricultural University, 61 Daizong Street, Tai'an, Shandong, P.R. China.

The black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), has been considered a major insect pest in China that causes damage to corn at the seeding stage. The present study measured the lethal and sublethal effects of the neonicotinoid insecticide clothianidin on A. ipsilon. Clothianidin, incorporated using an artificial diet, exhibited signs of active toxicity on fourth-instar larvae of A. ipsilon, with a 50%-lethal concentration (LC50) of 27.77 µg/g. Clothianidin at the LC20 and LC40 levels impaired the normal development of A. ipsilon by prolonging the larval period, decreasing the rate of pupation and eclosion, reducing longevity, shortening the oviposition period, and reducing the fecundity of female adults. Consequently, these effects resulted in the reduction of some population parameter values of A. ipsilon, including the intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R0), along with an increase in the mean generation time (T). However, stimulatory effects, i.e., hormesis, on reproduction were observed in A. ipsilon exposed to an LC5 level based on the fecundity (2,213.62 eggs per female) and net reproductive rate (R0) (863.04 offspring per individual), which were significantly higher than values in the control group (1,344.77 eggs per female and 591.82 offspring per individual). Therefore, the results obtained in this study may assist in the development of optimized integrated pest management strategies, although the results require further study for corroboration under real cropping conditions.
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http://dx.doi.org/10.1093/jee/toy254DOI Listing
December 2018

Cyantraniliprole seed treatment efficiency against Agrotis ipsilon (Lepidoptera: Noctuidae) and residue concentrations in corn plants and soil.

Pest Manag Sci 2019 May 1;75(5):1464-1472. Epub 2019 Feb 1.

College of Plant Protection and College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, China.

Background: The black cutworm Agrotis ipsilon is the most destructive early season insect pest of corn. In this study, the control efficiency of cyantraniliprole seed treatment against A. ipsilon was evaluated, and the residual concentrations of cyantraniliprole and its metabolite J9Z38 in the stalks of corn seedlings and soil were investigated.

Results: Plant pot experiments showed that A. ipsilon larval mortality was greater than 92% and that the percentage of corn seedlings damaged by A. ipsilon was less than 24% when corn seeds were treated with cyantraniliprole at 2 and 4 g AI kg seed. Cyantraniliprole seed treatment at a dosage of 2 g AI kg seed significantly reduced A. ipsilon infestation compared to chlorantraniliprole and clothianidin seed treatments in corn fields. Cyantraniliprole seed treatment resulted in more persistent control efficiency of A. ipsilon in spring than in summer. Cyantraniliprole and J9Z38 residues in corn stalks and soil degraded more slowly in the spring than in the summer.

Conclusion: Cyantraniliprole used as a seed treatment can protect corn plants from A. ipsilon infestations throughout the seedling stage. The high biological activity of cyantraniliprole was consistent with the residue levels of cyantraniliprole in the corn stalks and soil. © 2018 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.5269DOI Listing
May 2019

How We Should Assess the Delivery of End-Of-Life Care in General Practice? A Systematic Review.

J Palliat Med 2018 Aug 21. Epub 2018 Aug 21.

1 School of Population and Global Health, University of Western Australia , Perth, Western Australia, Australia .

Background: The majority of end-of-life (EOL) care occurs in general practice. However, we still have little knowledge about how this care is delivered or how it can be assessed and supported.

Aim: (i) To review the existing evaluation tools used for assessment of the delivery of EOL care from the perspective of general practice; (ii) To describe how EOL care is provided in general practice; (iii) To identify major areas of concern in providing EOL care in this context.

Design: A systematic review.

Data Sources: Systematic searches of major electronic databases (Medline, EMBASE, PsycINFO, and CINAHL) from inception to 2017 were used to identify evaluation tools focusing on organizational structures/systems and process of end-of-life care from a general practice perspective.

Results: A total of 43 studies representing nine evaluation tools were included. A relatively restricted focus and lack of validation were common limitations. Key general practitioner (GP) activities assessed by the evaluation tools were summarized and the main issues in current GP EOL care practice were identified.

Conclusions: The review of evaluation tools revealed that GPs are highly involved in management of patients at the EOL, but there are a range of issues relating to the delivery of care. An EOL care registration system integrated with electronic health records could provide an optimal approach to address the concerns about recall bias and time demands in retrospective analyses. Such a system should ideally capture the core GP activities and any major issues in care provision on a case-by-case basis.
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http://dx.doi.org/10.1089/jpm.2018.0194DOI Listing
August 2018

Sublethal effects of chlorfenapyr on the life table parameters, nutritional physiology and enzymatic properties of Bradysia odoriphaga (Diptera: Sciaridae).

Pestic Biochem Physiol 2018 Jun 6;148:93-102. Epub 2018 Apr 6.

Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address:

Bradysia odoriphaga (Diptera: Sciaridae) is the major pest affecting Chinese chive production. Chlorfenapyr is a halogenated pyrrole-based pro-insecticide that is currently used to control insects and mites on a variety of crops. In the present study, fourth-instar larvae of B. odoriphaga were exposed to chlorfenapyr at LC, LC and LC concentrations. The developmental duration of the treated larvae was not significantly different, but fecundity was significantly increased in the LC and LC treatment groups compared with the control group. The population parameters of the LC treatment group were increased significantly, whereas those of the LC treatment group were reduced significantly compared with the control. The food consumption by larvae and pupal weight were significantly increased under the LC treatment and decreased under the LC treatment compared with the control. Moreover, chlorfenapyr decreased the lipid, carbohydrate and trehalose contents significantly, whereas the total protein content was increased compared with the control. Additionally, the activities of protease, lipase and trehalase were significantly decreased. Chlorfenapyr treatment for 24 h also induced the activities of glutathione S-transferase (GST), carboxylesterase (CarE) and O-demethylation. The results of this study suggest that low lethal concentrations of chlorfenapyr can affect oviposition, population development, the activities of digestion and detoxification enzymes, and nutrient accumulation in B. odoriphaga. This study provides valuable information for the assessment and rational application of chlorfenapyr for effective control of this pest.
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http://dx.doi.org/10.1016/j.pestbp.2018.04.003DOI Listing
June 2018

Author Correction: Grain Yield, Starch Content and Activities of Key Enzymes of Waxy and Non-waxy Wheat (Triticum aestivum L.).

Sci Rep 2018 May 1;8(1):7090. Epub 2018 May 1.

Jiangsu Key Lab. of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops/Wheat Research Institute, Yangzhou Univ., Yangzhou, 225009, China.

A correction to this article has been published and is linked from the HTML version of this paper. The error has not been fixed in the paper.
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http://dx.doi.org/10.1038/s41598-018-25219-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928097PMC
May 2018

Sex- and Tissue-Specific Expression Profiles of Odorant Binding Protein and Chemosensory Protein Genes in (Diptera: Sciaridae).

Front Physiol 2018 3;9:107. Epub 2018 Apr 3.

College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, China.

is an agricultural pest insect affecting the production of Chinese chive and other liliaceous vegetables in China, and it is significantly attracted by sex pheromones and the volatiles derived from host plants. Despite verification of this chemosensory behavior, however, it is still unknown how recognizes these volatile compounds on the molecular level. Many of odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play crucial roles in olfactory perception. Here, we identified 49 OBP and 5 CSP genes from the antennae and body transcriptomes of female and male adults of , respectively. Sequence alignment and phylogenetic analysis among Dipteran OBPs and CSPs were analyzed. The sex- and tissue-specific expression profiles of 54 putative chemosensory genes among different tissues were investigated by quantitative real-time PCR (qRT-PCR). qRT-PCR analysis results suggested that 22 OBP and 3 CSP genes were enriched in the antennae, indicating they might be essential for detection of general odorants and pheromones. Among these antennae-enriched genes, nine OBPs () were enriched in the male antennae and may play crucial roles in the detection of sex pheromones. Moreover, some OBP and CSP genes were enriched in non-antennae tissues, such as in the legs ( and ), wings (), abdomens and thoraxes (), and heads ( and ), suggesting that these genes might be involved in olfactory, gustatory, or other physiological processes. Our findings provide a starting point to facilitate functional research of these chemosensory genes in at the molecular level.
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http://dx.doi.org/10.3389/fphys.2018.00107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891581PMC
April 2018