Publications by authors named "Jin Wang"

4,057 Publications

  • Page 1 of 1

Rational Design of Peptides Derived from Odorant-Binding Proteins for SARS-CoV-2-Related Volatile Organic Compounds Recognition.

Molecules 2022 Jun 18;27(12). Epub 2022 Jun 18.

Faculty of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3-1-1, Tsushima-Naka, Kitaku, Okayama 700-8530, Japan.

Peptides are promising molecular-binding elements and have attracted great interest in novel biosensor development. In this study, a series of peptides derived from odorant-binding proteins (OBPs) were rationally designed for recognition of SARS-CoV-2-related volatile organic compounds (VOCs). Ethanol, nonanal, benzaldehyde, acetic acid, and acetone were selected as representative VOCs in the exhaled breath during the COVID-19 infection. Computational docking and prediction tools were utilized for OBPs peptide characterization and analysis. Multiple parameters, including the docking model, binding affinity, sequence specification, and structural folding, were investigated. The results demonstrated a rational, rapid, and efficient approach for designing breath-borne VOC-recognition peptides, which could further improve the biosensor performance for pioneering COVID-19 screening and many other applications.
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http://dx.doi.org/10.3390/molecules27123917DOI Listing
June 2022

The Agronomic Traits, Alkaloids Analysis, FT-IR and 2DCOS-IR Spectroscopy Identification of the Low-Nicotine-Content Nontransgenic Tobacco Edited by CRISPR-Cas9.

Molecules 2022 Jun 14;27(12). Epub 2022 Jun 14.

Yunnan Key Laboratory of Tobacco Chemistry, Research and Development Center, China Tobacco Yunnan Industrial Co., Ltd., Kunming 650231, China.

In this study, the agricultural traits, alkaloids content and Fourier transform infrared spectroscopy (FT-IR) and two-dimensional correlation infrared spectroscopy (2DCOS-IR) analysis of the tobacco after Berberine Bridge Enzyme-Like Proteins (BBLs) knockout were investigated. The knockout of BBLs has limited effect on tobacco agricultural traits. After the BBLs knockout, nicotine and most alkaloids are significantly reduced, but the content of myosmine and its derivatives increases dramatically. In order to identify the gene editing of tobacco, principal component analysis (PCA) was performed on the FT-IR and 2DCOS-IR spectroscopy data. The results showed that FT-IR can distinguish between tobacco roots and leaves but cannot classify the gene mutation tobacco from the wild one. 2DCOS-IR can enhance the characteristics of the samples due to the increased apparent resolution of the spectra. Using the autopeaks in the synchronous map for PCA analysis, we successfully identified the mutants with an accuracy of over 90%.
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http://dx.doi.org/10.3390/molecules27123817DOI Listing
June 2022

A sliver deposition signal-enhanced optical biomolecular detection device based on reduced graphene oxide.

Talanta 2022 Jun 17;249:123691. Epub 2022 Jun 17.

Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics and TEDA Applied Physics, Nankai University, Tianjin, 300071, PR China. Electronic address:

The development of high-sensitive biomolecular detection system is of great significance for diseases early diagnosis. The novel optical sensor based on the polarization-sensitive absorption of graphene has a great potential in biological detection. However, the detection sensitivity of the device can hardly meet the needs of clinical analysis currently. This study applies sliver deposition signal amplification to the optical biomolecular detection device based on reduced graphene oxide for the sensitive immunoassay. In redox cycling enzymatic silver deposition reaction, the more alkaline phosphatase label bound on chip surface will cause a faster silver deposition rate. The specific antibody detection confirms that the sliver deposition can enhance the detection signal significantly. In cardiac biomarker Creatine Kinase-MB measurement, the minimum detection concentration is 0.1 ng/mL. To be more important, within the range from detection limit to 10 ng/mL, the signal intensity is highly correlated with target protein concentration, so the biomolecular detection device can meet clinical assay requirements. The signal-enhanced optical biomolecular detection device based on reduced graphene oxide shows excellent sensitivity and selectivity, and provides a new strategy for biomolecules detection, which can be applied in diseases accurate prediction and diagnosis.
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http://dx.doi.org/10.1016/j.talanta.2022.123691DOI Listing
June 2022

Ultrasimple and Ultrafast Method of Optical Modulation by Perovskite Quantum Dot Attachment to a Graphene Surface.

ACS Omega 2022 Jun 3;7(23):19606-19613. Epub 2022 Jun 3.

The School of Physics and Optoelectronics, Faculty of Science, Beijing University of Technology, Beijing 100124, China.

Optical modulation is the process of modifying the structure and elemental composition of materials so that the main optical parameters, including amplitude, frequency, and phase, are changed. Currently, much research attention has been directed toward ultrafast dynamics, but the process of modulation is often complex. To simplify the optical modulation process and improve the optical properties of perovskites for semiconductor quantum dot (QD) lasers, the process and physical mechanism underlying graphene QD ultrafast modulation of the optical properties of perovskite CsPbBr QDs were investigated. The typical cubic structure and square shape of CsPbBr QDs were characterized by transmission electron microscopy and X-ray diffraction, respectively. A luminescent peak centered near 540 nm and Stokes shift of 21.34 nm of CsPbBr QDs without graphene QDs were measured by absorption and photoluminescence spectroscopy. A maximum modulation shift of 133 nm and a modulation depth of 900% were achieved in CsPbBr with graphene. The results indicated that graphene QDs had the best modulation effect on perovskites when the drop volume was 0.05 mL. The process of ultrafast optical modulation via graphene QDs occurring within 1 ps was confirmed by the transient absorption spectrum. The modulation mechanism of graphene to perovskites is presented for guidance. This paper can be used as a reference for the optical modulation of perovskite materials.
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http://dx.doi.org/10.1021/acsomega.2c01310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202059PMC
June 2022

Peripheral Organ Injury After Stroke.

Front Immunol 2022 1;13:901209. Epub 2022 Jun 1.

Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.

Stroke is a disease with high incidence, mortality and disability rates. It is also the main cause of adult disability in developed countries. Stroke is often caused by small emboli on the inner wall of the blood vessels supplying the brain, which can lead to arterial embolism, and can also be caused by cerebrovascular or thrombotic bleeding. With the exception of recombinant tissue plasminogen activator (rt-PA), which is a thrombolytic drug used to recanalize the occluded artery, most treatments have been demonstrated to be ineffective. Stroke can also induce peripheral organ damage. Most stroke patients have different degrees of injury to one or more organs, including the lung, heart, kidney, spleen, gastrointestinal tract and so on. In the acute phase of stroke, severe inflammation occurs in the brain, but there is strong immunosuppression in the peripheral organs, which greatly increases the risk of peripheral organ infection and aggravates organ damage. Nonneurological complications of stroke can affect treatment and prognosis, may cause serious short-term and long-term consequences and are associated with prolonged hospitalization and increased mortality. Many of these complications are preventable, and their adverse effects can be effectively mitigated by early detection and appropriate treatment with various medical measures. This article reviews the pathophysiological mechanism, clinical manifestations and treatment of peripheral organ injury after stroke.
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http://dx.doi.org/10.3389/fimmu.2022.901209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200619PMC
June 2022

Long-term drought aggravates instability of alpine grassland productivity to extreme climatic event.

Ecology 2022 Jun 19:e3792. Epub 2022 Jun 19.

Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China.

The frequency and severity of extreme weather events are increasing and expected to increase more in the future together with global change. However, how extreme events and global change factors interactively influence community structures and ecosystem processes is largely unknown. Here, we investigated responses of temporal stability and resilience of aboveground net primary productivity (ANPP) of an alpine meadow to an extreme flooding event under different treatments of experimental drought and clipping. We found that ecosystems that were exposed to drought treatments for three years significantly decreased temporal stability of community productivity but increased resilience to flooding, whereas their resistance to or recovery from flooding did not change. Neither clipping nor its interaction with drought altered responses of these community stability metrics to flooding. Drought treatments significantly decreased plant species richness, asynchrony and dominant species stability, leading to the decrease in temporal stability and the increase in resilience in response to the extreme flooding event. We also revealed that the change in species asynchrony was the dominant impact pathway determining the responses of resilience and temporal stability to flooding. Our results highlight that the alpine grassland experiencing multi-year drought may aggravate instability of community productivity to extreme climatic events by reducing species asynchrony.
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http://dx.doi.org/10.1002/ecy.3792DOI Listing
June 2022

Correction to: N-myc Downstream-Regulated Gene 2 (Ndrg2): A Critical Mediator of Estrogen-Induced Neuroprotection Against Cerebral Ischemic Injury.

Mol Neurobiol 2022 Jun 18. Epub 2022 Jun 18.

Department of Anesthesiology, The First Medical Center of Chinese PLA General Hospital, Beijing, 100853, China.

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http://dx.doi.org/10.1007/s12035-022-02921-4DOI Listing
June 2022

Preparation of Janus nanosheets composed of gold/palladium nanoparticles and reduced graphene oxide for highly efficient emulsion catalysis.

J Colloid Interface Sci 2022 May 31;625:59-69. Epub 2022 May 31.

Key Laboratory of Flexible Electronics (KLoFE) and Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, China; National Laboratory of Solid State Microstructures, Nanjing University, Nanjing 210093, China. Electronic address:

Interface/emulsion catalysis is an effective approach for many organic and inorganic chemical reactions. Positioning catalysts at the interface is essential for highly efficient interface/emulsion catalysis. The sheet-like Janus nanostructures may have great advantages in stabilizing emulsions and improving reaction efficiency. In this work, by using a simple emulsion reaction and photodeposition, we successfully prepare the Janus nanosheets with opposite sides composed of AuNPs/PdNPs and reduced graphene oxide (rGO) respectively, referred to as AuNP-rGO/PdNP-rGO Janus nanosheets. Due to the distinct hydrophilicities on opposite sides, the prepared Janus nanosheets demonstrate high efficiency in emulsion stabilization and emulsion catalysis. By introducing the prepared PdNP-rGO and AuNP-rGO Janus nanosheets in emulsion reactions, highly efficient hydrogenation of p-nitroanisole and photocatalytic degradation of Nile red are demonstrated. This work provides a simple and cost-effective method to prepare Janus nanosheets and realize highly efficient emulsion reactions, and this strategy can be extended to promote many other interface/emulsion reactions.
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http://dx.doi.org/10.1016/j.jcis.2022.05.163DOI Listing
May 2022

The Safety, Toleration, and Pharmacokinetics of Two Intravenous Voriconazole Formulations in Healthy Chinese Volunteers After Increasing Dose Administrations.

Clin Pharmacol Drug Dev 2022 Jun 16. Epub 2022 Jun 16.

Department of Clinical Pharmacology, Aerospace Center Hospital, Beijing, China.

Sulfobutyl ether-beta-cyclodextrin sodium salt contained in the marketed intravenous voriconazole injection as a solubilizer may cause harmful accumulations. This study aimed to evaluate the safety, tolerability, and pharmacokinetics (PKs) of two intravenous voriconazole formulations containing excipients from different manufacturers using increasing dose administrations in healthy Chinese volunteers. A randomized, double-blind, placebo-controlled trial was conducted in three cohorts with 42 healthy Chinese volunteers. Each cohort of 14 volunteers was allocated in proportion (8:4:2) to test the formulation, reference voriconazole, or placebo successively by single-dose then multiple-dose administrations of 3, 4, and 6 mg/kg. Forty-one volunteers completed all drug administrations. The pharmacokinetics of test formulations are characterized by high interindividual variability (coefficient of variance of C up to 68.0%, AUC up to 70.2%, and nonlinear PKs with a regression coefficient of C  = 1.31 and AUC  = 1.75 in a single dose). In the steady state, R of the test drug versus reference drug of the 3, 4, and 6 mg/kg dose group were 5.2 and 5.3, 5.6 and 6.3, and 5.8 and 5.5, respectively, and Rc were 2.5 and 2.7, 2.6 and 3.1, and 2.8 and 2.6, respectively. Eighty-three adverse events with 37 transient visual disturbances were mild. PKs with high interindividual variability, nonlinear characteristics, and significant dose-dependent accumulation were comparable between the two formulations. Overall, the safety of the test formulation was acceptable.
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http://dx.doi.org/10.1002/cpdd.1126DOI Listing
June 2022

The rate of thermodynamic cost against adiabatic and nonadiabatic fluctuations of a single gene circuit in Drosophila embryos.

J Chem Phys 2022 Jun;156(22):225101

Department of Chemistry and of Physics and Astronomy, Stony Brook University, Stony Brook, New York 11794-3400, USA.

We study the stochastic dynamics of the externally regulating gene circuit as an example of an eve-skipped gene stripe in the development of Drosophila. Three gene regulation regimes are considered: an adiabatic phase when the switching rate of the gene from the OFF to ON state is faster than the rate of mRNA degradation; a nonadiabatic phase when the switching rate from the OFF to ON state is slower than that of the mRNA degradation; and a bursting phase when the gene switching is fast and transcription is very fast, while the ON state probability is very low. We found that the rate of thermodynamic cost quantified by the entropy production rate can suppress the fluctuations of the gene circuit. A higher (lower) rate of thermodynamic cost leads to reduced (increased) fluctuations in the number of gene products in the adiabatic (nonadiabatic) regime. We also found that higher thermodynamic cost is often required to sustain the emergence of more gene states and, therefore, more heterogeneity coming from genetic mutations or epigenetics. We also study the stability of the gene state using the mean first passage time from one state to another. We found the monotonic decrease in time, i.e., in the stability of the state, in the transition from the nonadiabatic to adiabatic regimes. Therefore, as the higher rate of thermodynamic cost suppresses the fluctuations, higher stability requires higher thermodynamics cost to maintain.
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http://dx.doi.org/10.1063/5.0091710DOI Listing
June 2022

Explainable detection of adverse drug reaction with imbalanced data distribution.

PLoS Comput Biol 2022 Jun 15;18(6):e1010144. Epub 2022 Jun 15.

School of Information Science and Engineering, Yunnan University, Kunming, China.

Analysis of health-related texts can be used to detect adverse drug reactions (ADR). The greatest challenge for ADR detection lies in imbalanced data distributions where words related to ADR symptoms are often minority classes. As a result, trained models tend to converge to a point that strongly biases towards the majority class and then ignores the minority class. Since the most used cross-entropy criteria is an approximation to accuracy, the model focuses more readily on the majority class to achieve high accuracy. To address this issue, existing methods apply either oversampling or down-sampling strategies to balance the data distribution and exploit the most difficult samples of the minority class. However, increasing or reducing the number of individual tokens alone in sequence labeling tasks will result in the loss of the syntactic relations of the sentence. This paper proposes a weighted variant of conditional random field (CRF) for data-imbalanced sequence labeling tasks. Such a weighting strategy can alleviate data distribution imbalances between majority and minority classes. Instead of using softmax in the output layer, the CRF can capture the relationship of labels between tokens. The locally interpretable model-agnostic explanations (LIME) algorithm was applied to investigate performance differences between models with and without the weighted loss function. Experimental results on two different ADR tasks show that the proposed model outperforms previously proposed sequence labeling methods.
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http://dx.doi.org/10.1371/journal.pcbi.1010144DOI Listing
June 2022

TLR4 aggravates microglial pyroptosis by promoting DDX3X-mediated NLRP3 inflammasome activation via JAK2/STAT1 pathway after spinal cord injury.

Clin Transl Med 2022 Jun;12(6):e894

Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, P. R. China.

Background: Toll-like receptor 4 (TLR4) participates in the initiation of neuroinflammation in various neurological diseases, including central nervous system injuries. NLR family pyrin domain containing 3 (NLRP3) inflammasome-mediated microglial pyroptosis is crucial for the inflammatory response during secondary spinal cord injury (SCI). However, the underlying mechanism by which TLR4 regulates NLRP3 inflammasome activation and microglial pyroptosis after SCI remains uncertain.

Methods: We established an in vivo mouse model of SCI using TLR4-knockout (TLR4-KO) and wild-type (WT) mice. The levels of pyroptosis, tissue damage and neurological function recovery were evaluated in the three groups (Sham, SCI, SCI-TLR4-KO). To identify differentially expressed proteins, tandem mass tag (TMT)-based proteomics was conducted using spinal cord tissue between TLR4-KO and WT mice after SCI. For our in vitro model, mouse microglial BV2 cells were exposed to lipopolysaccharides (1 µg/ml, 8 h) and adenosine triphosphate (ATP) (5 mM, 2 h) to induce pyroptosis. A series of molecular biological experiments, including Western blot (WB), real-time quantitative polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), immunohistochemical (IHC), chromatin immunoprecipitation (ChIP), Dual-Luciferase Reporter assay (DLA) and co-immunoprecipitation (Co-IP), were performed to explore the specific mechanism of microglial pyroptosis in vivo and in vitro.

Results: Our results indicated that TLR4 promoted the expression of dead-box helicase 3 X-linked (DDX3X), which mediated NLRP3 inflammasome activation and microglial pyroptosis after SCI. Further analysis revealed that TLR4 upregulated the DDX3X/NLRP3 axis by activating the JAK2/STAT1 signalling pathway, and importantly, STAT1 was identified as a transcription factor promoting DDX3X expression. In addition, we found that biglycan was increased after SCI and interacted with TLR4 to jointly regulate microglial pyroptosis through the JAK2/STAT1/DDX3X/NLRP3 axis after SCI.

Conclusion: Our study preliminarily identified a novel mechanism by which TLR4 regulates NLRP3 inflammasome-mediated microglial pyroptosis in response to SCI-providing a novel and promising therapeutic target for SCI.
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http://dx.doi.org/10.1002/ctm2.894DOI Listing
June 2022

A Long Short-Term Memory Network for Plasma Diagnosis from Langmuir Probe Data.

Sensors (Basel) 2022 Jun 4;22(11). Epub 2022 Jun 4.

Institute of Space Sciences, Shandong University, Weihai 264209, China.

Electrostatic probe diagnosis is the main method of plasma diagnosis. However, the traditional diagnosis theory is affected by many factors, and it is difficult to obtain accurate diagnosis results. In this study, a long short-term memory (LSTM) approach is used for plasma probe diagnosis to derive electron density () and temperature () more accurately and quickly. The LSTM network uses the data collected by Langmuir probes as input to eliminate the influence of the discharge device on the diagnosis that can be applied to a variety of discharge environments and even space ionospheric diagnosis. In the high-vacuum gas discharge environment, the Langmuir probe is used to obtain current-voltage (I-V) characteristic curves under different and . A part of the data input network is selected for training, the other part of the data is used as the test set to test the network, and the parameters are adjusted to make the network obtain better prediction results. Two indexes, namely, mean squared error (MSE) and mean absolute percentage error (MAPE), are evaluated to calculate the prediction accuracy. The results show that using LSTM to diagnose plasma can reduce the impact of probe surface contamination on the traditional diagnosis methods and can accurately diagnose the underdense plasma. In addition, compared with , the diagnosis result output by LSTM is more accurate.
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http://dx.doi.org/10.3390/s22114281DOI Listing
June 2022

Is Subjective Age Associated with Physical Fitness in Community-Dwelling Older Adults?

Int J Environ Res Public Health 2022 Jun 3;19(11). Epub 2022 Jun 3.

Faculty of Sport Science, Ningbo University, Ningbo 315211, China.

Although subjective age has been associated with a range of health-related outcomes, there has been little systematic study on the relationship between the subjective age and physical fitness in a given population. The purpose of this study was to determine the prospective association between subjective age and physical fitness in community-dwelling older adults. A sample of 276 older people who lived in the community was studied. Subjective age was measured by a face-to-face interview. Grip strength, balancing on one leg with eyes open, the 30 s chair stand test, 4 m habitual walk, and 6 min walk test were measured to reflect physical fitness. Results indicated that the felt younger older adults had a higher level of physical fitness compared to their felt older and felt the same counterparts. Multiple linear regression analysis indicated that all the measured physical fitness items were significantly associated with subjective age in older men. All of the measured physical fitness items except for the 4 m habitual walk were remarkably related to subjective age in older women. The findings suggest that subjective age is closely associated with physical fitness in community-dwelling older adults. Much attention should be paid to the promotion of physical fitness to improve the subjective age of older adults.
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http://dx.doi.org/10.3390/ijerph19116841DOI Listing
June 2022

Lysosome lipid signalling from the periphery to neurons regulates longevity.

Nat Cell Biol 2022 Jun 9;24(6):906-916. Epub 2022 Jun 9.

Huffington Center on Aging, Baylor College of Medicine, Houston, TX, USA.

Lysosomes are key cellular organelles that metabolize extra- and intracellular substrates. Alterations in lysosomal metabolism are implicated in ageing-associated metabolic and neurodegenerative diseases. However, how lysosomal metabolism actively coordinates the metabolic and nervous systems to regulate ageing remains unclear. Here we report a fat-to-neuron lipid signalling pathway induced by lysosomal metabolism and its longevity-promoting role in Caenorhabditis elegans. We discovered that induced lysosomal lipolysis in peripheral fat storage tissue upregulates the neuropeptide signalling pathway in the nervous system to promote longevity. This cell-non-autonomous regulation is mediated by a specific polyunsaturated fatty acid, dihomo-γ-linolenic acid, and LBP-3 lipid chaperone protein transported from the fat storage tissue to neurons. LBP-3 binds to dihomo-γ-linolenic acid, and acts through NHR-49 nuclear receptor and NLP-11 neuropeptide in neurons to extend lifespan. These results reveal lysosomes as a signalling hub to coordinate metabolism and ageing, and lysosomal signalling mediated inter-tissue communication in promoting longevity.
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http://dx.doi.org/10.1038/s41556-022-00926-8DOI Listing
June 2022

MicroRNA-9a-5p inhibits mucosal barrier injury in inflammatory bowel disease.

J Biochem Mol Toxicol 2022 Jun 7:e23131. Epub 2022 Jun 7.

The Second Affiliated Hospital of Jiaxing University, Jiaxing, China.

Our previous research found that FOXO1 aggravates the mucosal barrier injury in inflammatory bowel disease (IBD) by regulating TLR4/MD2 signaling. In this study, we further reveal the mechanism of action whereby miRNA-9a-5p inhibits the mucosal barrier injury after regulating FOXO1. An IBD model was established in C57BL/6N mice using dextran sulfate sodium (DSS). The effects of endogenous miRNA-9a-5p were mimicked/antagonized by intraperitoneally injecting miRNA-9a-5p agomir and antagomir. Body weights of mice were monitored and the disease activity index scores were assessed. H&E staining was performed to examine pathological changes, while immunohistochemical (IHC) staining was conducted to measure the expressions of TJ proteins (ZO-1, Occludin), as well as FOXO1 and TLR4. The mucosal permeability was assessed by FITC-D, the tissue inflammatory cytokines were detected by enzyme linked immunosorbent assay, and the expressions of ZO-1 and Occludin were measured through Western blot analysis. Caco-2 cells were cultured in vitro to establish a monolayer model of the mucosal barrier. TNF-α was used to induce the cell damage, while agomir and antagomir were transfected to mimic/antagonize the miRNA-9a-5p action, followed by determination of barrier permeability. There was a targeted regulatory relationship between MiRNA-9a-5p and FOXO1. MiRNA-9a-5p could suppress the FOXO1 expression, thereby downregulating the TLR4 signaling activation, inhibiting the mucosal barrier injury, and elevating the expressions of TJ proteins. We also found in Caco-2 cells that miRNA-9a-5p could protect cells from inflammatory injury and reduce permeability. In rescue experiments, the effect of agomir was found inhibited by the overexpression of FOXO1 in agomir-treated cells. This study found that miRNA-9a-5p could inhibit the TLR4 signaling activation by targeting FOXO1, thereby exerting a protective effect on the mucosal barrier injury in IBD.
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http://dx.doi.org/10.1002/jbt.23131DOI Listing
June 2022

A plasmonic Au-Ag janus nanoprobe for monitoring endogenous hydrogen sulfide generation in living cells.

Biosens Bioelectron 2022 May 25;213:114422. Epub 2022 May 25.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, PR China. Electronic address:

Hydrogen sulfide (HS) is an essential intracellular gasotransmitter undertaking numerous signaling roles and physiological effects. Herein, an ingenious anisotropic gold (Au)-silver (Ag) Janus plasmonic nanoprobe was constructed based on the dark-field light scattering imaging for the detection of exogenous and endogenous HS. In the presence of sulfide, the Ag islands as the sensing agent were susceptible to oxidation to AgS, which induced changes in the longitudinal localized surface plasmon resonance (LSPR) properties of Au-Ag Janus nanoparticles (JNPs), resulting in remarkable and distinctive scattering color changes as well as spectral shifts. The JNPs exhibited satisfying anti-interference capability and a good linear relationship with a low detection limit of 0.11 nM. Additionally, this distinct nanoprobe was successfully applied to monitor endogenous HS as well as map local variations of HS in real-time with its unique intuition and sensitivity in intracellular imaging applications. This work provided a promising application in the plasmonic detection of intracellular HS and paved insight into the HS-related biological process.
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http://dx.doi.org/10.1016/j.bios.2022.114422DOI Listing
May 2022

Neuronal GPER Participates in Genistein-Mediated Neuroprotection in Ischemic Stroke by Inhibiting NLRP3 Inflammasome Activation in Ovariectomized Female Mice.

Mol Neurobiol 2022 Jun 6. Epub 2022 Jun 6.

Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, 710032, China.

Estrogen replacement therapy (ERT) is potentially beneficial for the prevention and treatment of postmenopausal cerebral ischemia but inevitably increases the risk of cerebral hemorrhage and breast cancer when used for a long period of time. Genistein, a natural phytoestrogen, has been reported to contribute to the recovery of postmenopausal ischemic stroke with reduced risks. However, the underlying mechanism of genistein-mediated neuroprotection remains unclear. We reported that genistein exerted significant neuroprotective effects by enhancing the expression of neuronal G protein-coupled estrogen receptor (GPER) in the ischemic penumbra after cerebral reperfusion in ovariectomized (OVX) mice, and this effect was achieved through GPER-mediated inhibition of nod-like receptor protein 3 (NLRP3) inflammasome activation. In addition, we found that peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) was the pivotal molecule that participated in GPER-mediated inhibition of NLRP3 inflammasome activation in OVX mice after ischemia/reperfusion (I/R) injury. Our data suggest that the neuronal GPER/PGC-1α pathway plays an important role in genistein-mediated neuroprotection against I/R injury in OVX mice.
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http://dx.doi.org/10.1007/s12035-022-02894-4DOI Listing
June 2022

Irisin protects against sepsis-associated encephalopathy by suppressing ferroptosis via activation of the Nrf2/GPX4 signal axis.

Free Radic Biol Med 2022 Jul 1;187:171-184. Epub 2022 Jun 1.

Department of Anesthesiology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. Electronic address:

Sepsis-associated encephalopathy (SAE) is a common complication of severe sepsis. Irisin is a novel exercise-induced myokine involved in the regulation of adipose browning and thermogenesis. This study is designed to verify the existence of ferroptosis in the pathogenesis of SAE and demonstrate that irisin attenuated cognitive dysfunction in SAE mice via inhibition of hippocampus ferroptosis. A mouse SAE model was induced by cecal ligation and puncture (CLP) and in vitro model was established by LPS-stimulated hippocampus cells. Irisin were pre-treated in the models. We found that SAE triggered hippocampus ferroptosis, as evidenced by increasing ROS, iron content and MDA and reducing GSH level as well as altered ferroptosis-related protein (GPX4, ACSL4 and SLC7A11) expression, whereas irisin attenuated CLP-induced learning and memory dysfunction, neurologic severity score and hippocampus ferroptosis and microglial activation in SAE mice. However, the protective effect of irisin was eliminated by ferroptosis inducer Erastin. Consistently, irisin reduced ferroptosis and improved mitochondrial dysfunction in LPS-induced HT-22 cells, as evidenced by decreased lipid ROS and increased mitochondrial membrane potential. Furthermore, proteomics identified the differentially expressed proteins linked to ferroptosis in SAE. We also observed that irisin-mediated anti-ferroptosis was abolished by siRNA-Nrf2 or in Nrf2 mice. Transwell assay revealed that irisin could prevent the recruitment and chemotaxis of microglial cells induced by ferroptotic hippocampal cells. In conclusion, irisin could ameliorate inflammatory microenvironment in SAE by suppressing hippocampus ferroptosis via the Nrf2/GPX4 signaling pathway.
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http://dx.doi.org/10.1016/j.freeradbiomed.2022.05.023DOI Listing
July 2022

A reversible plasmonic nanoprobe for dynamic imaging of intracellular pH during endocytosis.

Chem Sci 2022 May 5;13(17):4893-4901. Epub 2022 Apr 5.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 China

Understanding the pH evolution during endocytosis is essential for our comprehension of the fundamental processes of biology as well as effective nanotherapeutic design. Herein, we constructed a plasmonic [email protected] core-shell nanoprobe, which possessed significantly different scattering properties under acidic and basic conditions. Encouragingly, the scattering signal of [email protected] nanoprobes displayed a positive linear correlation with the pH value not only in PBS but also in nigericin-treated cells. Ultimately, benefiting from the excellent response properties as well as the excellent biocompatibility and stability, the [email protected] nanoprobes have successfully enabled a dynamic assessment of the evolving pH in the endosomal package as the endosome matured from early to late, and eventually to the lysosome, by reporting scattering signal changes.
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http://dx.doi.org/10.1039/d2sc01069kDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067569PMC
May 2022

Evolution and functional diversification of catalase genes in the green lineage.

BMC Genomics 2022 Jun 1;23(1):411. Epub 2022 Jun 1.

College of Horticulture, Nanjing Agricultural University, Nanjing, China.

Background: Catalases (CATs) break down hydrogen peroxide into water and oxygen to prevent cellular oxidative damage, and play key roles in the development, biotic and abiotic stresses of plants. However, the evolutionary relationships of the plant CAT gene family have not been systematically reported.

Results: Here, we conducted genome-wide comparative, phylogenetic, and structural analyses of CAT orthologs from 29 out of 31 representative green lineage species to characterize the evolution and functional diversity of CATs. We found that CAT genes in land plants were derived from core chlorophytes and detected a lineage-specific loss of CAT genes in Fabaceae, suggesting that the CAT genes in this group possess divergent functions. All CAT genes were split into three major groups (group α, β1, and β2) based on the phylogeny. CAT genes were transferred from bacteria to core chlorophytes and charophytes by lateral gene transfer, and this led to the independent evolution of two types of CAT genes: α and β types. Ten common motifs were detected in both α and β groups, and β CAT genes had five unique motifs, respectively. The findings of our study are inconsistent with two previous hypotheses proposing that (i) new CAT genes are acquired through intron loss and that (ii) the Cys-343 residue is highly conserved in plants. We found that new CAT genes in most higher plants were produced through intron acquisition and that the Cys-343 residue was only present in monocots, Brassicaceae and Pp_CatX7 in P. patens, which indicates the functional specificity of the CATs in these three lineages. Finally, our finding that CAT genes show high overall sequence identity but that individual CAT genes showed developmental stage and organ-specific expression patterns suggests that CAT genes have functionally diverged independently.

Conclusions: Overall, our analyses of the CAT gene family provide new insights into their evolution and functional diversification in green lineage species.
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http://dx.doi.org/10.1186/s12864-022-08621-6DOI Listing
June 2022

The Potential Circular RNAs Biomarker Panel and Regulatory Networks of Parkinson's Disease.

Front Neurosci 2022 13;16:893713. Epub 2022 May 13.

Department of Neurology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Parkinson's disease (PD) is a progressive neurodegenerative disease. It has been reported that circular RNAs (circRNAs) play important roles in several neurological diseases. However, the role and regulatory networks of circRNAs in PD are still largely unclear. In this study, we first compared the global expression level of circRNAs from patients with PD and controls using microarray, then the candidate circRNAs were validated in another PD cohort. The possible functions of these candidate circRNAs were analyzed using Gene Ontology (GO) analyses and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, and the regulatory networks of these candidate circRNAs were constructed through circRNA-miRNA-mRNA regulatory networks, protein-protein interaction (PPI) networks, and transcription factor-circRNA networks. The results indicated that hsa_circRNA_101275, hsa_circRNA_103730, and hsa_circRNA_038416 were significantly more highly expressed in patients with PD, while hsa_circRNA_102850 was lower expressed in patients with PD when compared with controls. A circRNA panel combining the four differentially expressed circRNA showed a high diagnostic ability to distinguish patients with PD from controls (AUC = 0.938). Furthermore, GO and KEGG analysis showed these candidate circRNAs were enriched in PI3K-Akt and MAPK signaling pathways. We established circRNA-miRNA-mRNA regulatory networks and identified 10 hub genes (, , , , , , , , , and ) were closely related to PD by using PPI network analysis. Besides, these circRNAs were predicted to be regulated through tyrosine hydroxylase (TH)-relevant transcription factors such as and . In conclusion, our results suggest that the circRNA panel and the established circRNA-miRNA-mRNA regulation networks might provide potential novel biomarkers and therapeutic targets for PD.
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http://dx.doi.org/10.3389/fnins.2022.893713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136065PMC
May 2022

One-step ultra-sensitive immunochromatographic strip authenticating an emergent fraud acetophenetidin in herbal tea.

Food Chem Toxicol 2022 Jul 25;165:113183. Epub 2022 May 25.

Guangdong Provincial Key Laboratory of Food Quality and Safety / Nation-Local Joint Engineering Research Center for Precision Machining and Safety of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Herbal tea is a highly popular and widely consumed beverage. However, a pain-relieving and fever-reducing drug, acetophenetidin, was recently found to illegally occur in herbal tea for a fraud purpose. Due to the potential health risk and urgent requirement for on-site screening method, a one-step and high specificity strip for identifying acetophenetidin was developed for the first time. Assisted by computational chemistry, four haptens were designed to prepare immunogens and coating antigens for antibody generation, and a specific antibody with ultra-sensitivity and high specificity was generated, showing half maximal inhibitory (IC) of 16.46 ng/mL for acetophenetidin, less than 3.5% of cross-reactivity to analogs by ELISA. A gold nanoparticles immunochromatographic strip was developed for detection of acetophenetidin in herbal tea, demonstrating a cut-off value of 160 ng/mL and a limit of detection of 1.63 ng/mL. The recovery rates were ranged from 102.2% to 106.1% with coefficient of variation between 2.21% and 7.20%. The analysis of real samples (n = 20) by the strip was well correlated with that of the confirmatory method, liquid chromatography-tandem mass spectrometry. The proposed strip has the potential to be used for rapid screening of acetophenetidin in herbal tea.
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http://dx.doi.org/10.1016/j.fct.2022.113183DOI Listing
July 2022

Restoration of appearance for women after aesthetic eyelash transplantation using a novel eyelash resection technique.

J Cosmet Dermatol 2022 May 30. Epub 2022 May 30.

Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Hair transplantation based on the follicular unit extraction provides a new opportunity to improve the appearance of patients with congenital sparse eyelashes. However, disparity between transplanted grafts and original eyelashes and the physiological characteristics of upper eyelid skin cause difficulties with this technique and result in low satisfaction. Removal of unsatisfactory eyelashes is indispensable for restoration of appearance and a second transplantation. Unfortunately, existing methods for hair removal have variable success rates, and hairs frequently regrow.

Objective: This article introduces an effective method to remove unsatisfactory eyelashes in patients with congenital sparse eyelashes who have undergone eyelash transplantation.

Methods: We used a new technique, which involves resection of eyelashes with a composite strip, to remove unsatisfactory eyelashes in patients who underwent eyelash transplantation. The demographic and clinical characteristics of patients were recorded. Outcomes evaluated included patient satisfaction, hair regrowth, and long-term complications.

Results: From 2017 to 2021, 10 patients (20 sides) underwent eyelash removal. All patients were highly satisfied with the outcomes. Unsatisfactory eyelashes were thoroughly removed, and none regrew during 1 year of follow-up. No complications were observed.

Conclusion: Strip composite eyelash excision is a safe and effective method for patients who have undergone unsatisfactory eyelash transplantation.
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http://dx.doi.org/10.1111/jocd.15130DOI Listing
May 2022

The Relationship Between Pre-existing Coronary Heart Disease and Cognitive Impairment Is Partly Explained by Reduced Left Ventricular Ejection Fraction in the Subjects Without Clinical Heart Failure: A Cross-Sectional Study.

Front Hum Neurosci 2022 11;16:835900. Epub 2022 May 11.

Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: Coronary heart disease (CHD) is closely associated with cognitive impairment, especially in severe cases of heart failure. However, it is unclear whether cardiac systolic function plays a role in the relationship between pre-existing CHD and cognitive impairment in subjects without clinical heart failure.

Methods: In total, 208 subjects from the First Affiliated Hospital of Xi'an Jiaotong University were recruited from June 2014 to January 2015, and were divided into CHD ( = 118) and non-CHD ( = 90) groups according to the inclusion and exclusion criteria. The global cognitive function of all subjects was assessed by the Mini-Mental State Examination (MMSE) and cognitive impairment was defined as the score lower than the cutoff value. Left ventricular ejection fraction (LVEF) was measured using transthoracic echocardiograms. The relationship among pre-existing CHD, LVEF, and cognitive impairment was analyzed by multivariate logistic regression.

Results: In total, 34 subjects met the criteria of cognitive impairment. Univariate analysis showed that the cognitive impairment prevalence in the CHD group was significantly higher than that in the non-CHD group (22.0 vs. 8.9%, = 0.011). Multivariate logistic analysis revealed that CHD was significantly associated with a higher risk of cognitive impairment (odds ratio [] = 3.284 [95% , 1.032-10.450], = 0.044) after adjusting for confounds except for LVEF. However, the OR of CHD decreased ( = 2.127 [95% , 0.624-7.254], = 0.228) when LVEF was further corrected as a continuous variable, and LVEF was negatively associated with the risk of cognitive impairment ( = 0.928 [95% , 0.882-0.976], = 0.004).

Conclusion: Pre-existing CHD is associated with a higher risk of cognitive impairment, and such an association can be considerably explained by reduced LVEF. An impaired cardiac systolic function may play a key role in the relationship between CHD and cognitive impairment among patients with pre-heart failure conditions.
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http://dx.doi.org/10.3389/fnhum.2022.835900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130859PMC
May 2022

An exploratory clinical trial of apatinib combined with intensity-modulated radiation therapy for patients with unresectable hepatocellular carcinoma.

Cancer Med 2022 May 27. Epub 2022 May 27.

Department of Clinical Oncology, Renmin Hospital of Wuhan University, Wuhan, China.

Purpose: To evaluate the clinical efficacy and safety of apatinib combined with intensity-modulated radiation therapy (IMRT) in patients with unresectable hepatocellular carcinoma (uHCC).

Materials And Methods: Open-label, single-arm, exploratory clinical trial of apatinib combined with IMRT for uHCC patients. Patients aged 18-75 years with adequate hematological, liver, and renal functions and Eastern Cooperative Oncology Group (ECOG) performance status of ≤2 were enrolled in this study from March 2017 to September 2020. Patients were received IMRT (biological effective dose: 46-60 Gy) and continuous apatinib (250-500 mg/day) oral administration until HCC progression or unacceptable toxic effects. The endpoints included progression-free survival (PFS), overall survival (OS), disease control rate (DCR), objective response rate (ORR), and safety. The trial registration number is ChiCTR-OPC-17011890.

Results: A total of 33 patients have taken part in the study. The median age was 58 years old (range 32-77), 27 (81.9%) patients were ECOG PS 0-1, and 28 (84.9%) patients were male. In addition, 25 (75.7%) patients suffered from hepatitis B, 32 cases (97.0%) were in Barcelona Clinic Liver Cancer (BCLC) Stages B-C, and eight (24.2%) had portal vein involvement. Moreover, 12 (36.4%) and 21 (63.6%) patients received apatinib as first-line and second or later-line therapy, respectively. The average follow-up was 11.4 months, the median PFS was 7.8 months (95% confidence interval: 3.9-11.7). The OS rates at 6 and 12 months were 96.7% and 66.2%. The ORR and DCR were 15.1% and 81.8%, respectively. Hepatic toxicity was the most common treatment-related adverse events in Grades 3-4 (12.1%). No radiation-induced liver disease and Grade 5 toxicity were recorded.

Conclusion: Apatinib combined with IMRT is a safe and effective method to improve PFS and DCR and has good anti-tumor activity in patients with uHCC.
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http://dx.doi.org/10.1002/cam4.4900DOI Listing
May 2022

Changes in the Structural Composition and Moisture-Adsorption Properties of Mechanically Rolled Bamboo Fibers.

Materials (Basel) 2022 May 11;15(10). Epub 2022 May 11.

International Centre for Bamboo and Rattan, Beijing 100102, China.

The chemical content, mechanical capability, and dimensional stability of bamboo fibers (BFs) are all directly related to the hygroscopic behavior, which is crucial for industrial applications. To support the utilization of BFs, the structural and chemical composition of BFs with different opening times after mechanical rolling were investigated in this study, and the Guggenheim-Anderson-de Boer (GAB) model was selected to predict their moisture-adsorption properties. The results showed that the length and diameter of the fibers gradually decreased with the increase in the number of openings, and the fibers gradually separated from bundles into single fibers. It was also observed that the treated BFs exhibited different equilibrium moisture contents (EMCs). BFs with a smaller number of openings had a higher hemicellulose content and more exposed parenchyma cells on the fibers, which increased the number of water adsorption sites. As the number of openings increased, the parenchyma cells on the fibers decreased, and the lignin content increased, which reduced the number of fiber moisture-adsorption sites and decreased the EMC of the fibers.
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http://dx.doi.org/10.3390/ma15103463DOI Listing
May 2022

Dynamic Changes in Ascorbic Acid Content during Fruit Development and Ripening of (an Ascorbate-Rich Fruit Crop) and the Associated Molecular Mechanisms.

Int J Mol Sci 2022 May 22;23(10). Epub 2022 May 22.

Institute of Pomology and Olericulture, College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

is one of the very few kiwifruit genotypes with extremely high ascorbic acid (AsA) content. However, a transcriptome atlas of this species is lacking. The accumulation of AsA during fruit development and ripening and the associated molecular mechanisms are still poorly understood. Herein, dynamic changes in AsA content at six different stages of fruit development and ripening were determined. AsA content of fruit reached 1108.76 ± 35.26 mg 100 g FW at full maturity. A high-quality, full-length (FL) transcriptome of was successfully constructed for the first time using third-generation sequencing technology. The transcriptome comprises 326,926 FL non-chimeric reads, 15,505 coding sequences, 2882 transcription factors, 18,797 simple sequence repeats, 3328 long noncoding RNAs, and 231 alternative splicing events. The genes involved in AsA biosynthesis and recycling pathways were identified and compared with those in different kiwifruit genotypes. The correlation between the AsA content and expression levels of key genes in AsA biosynthesis and recycling pathways was revealed. LncRNAs that participate in AsA-related gene expression regulation were also identified. Gene expression patterns in AsA biosynthesis and metabolism exhibited a trend similar to that of AsA accumulation. Overall, this study paves the way for genetic engineering to develop kiwifruits with super-high AsA content.
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http://dx.doi.org/10.3390/ijms23105808DOI Listing
May 2022

Inulin-Modified Liposomes as a Novel Delivery System for Cinnamaldehyde.

Foods 2022 May 18;11(10). Epub 2022 May 18.

Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China.

Cinnamaldehyde as an antioxidant was encapsulated in inulin-modified nanoliposomes in order to improve its physical and antioxidant stability. The microstructure, particle size and volume distribution of cinnamaldehyde liposomes were characterized by atomic force microscopy (AFM) and dynamic light scattering (DLS). The particle size and polydispersion index (PDI) values of the inulin modified liposomes were 72.52 ± 0.71 nm and 0.223 ± 0.031, respectively. The results showed that the liposomes after surface modification with inulin remained spherical. Raman and Fourier transform infrared (FTIR) spectra analysis showed that hydrogen bonds were formed between the inulin and the liposome membrane. Inulin binding also restricted the freedom of movement of lipid molecules and enhanced the order of the hydrophobic core of the membrane and the polar headgroup region in lipid molecules. Therefore, the addition of different concentrations of inulin influenced the permeability of the liposome bilayer membrane. However, when inulin was excessive, the capacity of the bilayer membrane to load the cinnamaldehyde was reduced, and the stability of the system was reduced. Additionally, the encapsulation efficiency (EE) and retention rate (RR) of cinnamaldehyde from inulin-modified liposomes during storage were determined. The EE value of the inulin modified liposomes was 70.71 ± 0.53%. The liposomes with 1.5% inulin concentration had the highest retention rate (RR) and the smallest particle size during storage at 4 °C. The addition of inulin also enhanced the thermal stability of the liposomes. Based on the results, the surface modification improved the oxidation stability of liposomes, especially the DPPH scavenging ability. In conclusion, these results might help to develop inulin as a potential candidate for the effective modification of the surface of liposomes and provide data and conclusions for it.
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http://dx.doi.org/10.3390/foods11101467DOI Listing
May 2022

N-myc Downstream-Regulated Gene 2 (Ndrg2): A Critical Mediator of Estrogen-Induced Neuroprotection Against Cerebral Ischemic Injury.

Mol Neurobiol 2022 May 27. Epub 2022 May 27.

Department of Anesthesiology, The First Medical Center of Chinese PLA General Hospital, Beijing, 100853, China.

Growing evidence indicates that estrogen plays a pivotal role in neuroprotection against cerebral ischemia, but the molecular mechanism of this protection is still elusive. N-myc downstream-regulated gene 2 (Ndrg2), an estrogen-targeted gene, has been shown to exert neuroprotective effects against cerebral ischemia in male mice. However, the role of Ndrg2 in the neuroprotective effect of estrogen remains unknown. In this study, we first detected NDRG2 expression levels in the cortex and striatum in both female and male mice with western blot analyses. We then detected cerebral ischemic injury by constructing middle cerebral artery occlusion and reperfusion (MCAO-R) models in Ndrg2 knockout or conditional knockdown female mice. We further implemented estrogen, ERα, or ERβ agonist replacement in the ovariectomized (OVX) Ndrg2 knockout or conditional knockdown female mice, then tested for NDRG2 expression, glial fibrillary acidic protein (GFAP) expression, and extent of cerebral ischemic injury. We found that NDRG2 expression was significantly higher in female than in male mice in both the cortex and striatum. Ndrg2 knockouts and conditional knockdowns showed significantly aggravated cerebral ischemic injury in female mice. Estrogen and ERβ replacement treatment (DPN) led to NDRG2 upregulation in both the cortex and striatum of OVX mice. Estrogen and DPN also led to GFAP upregulation in OVX mice. However, the effect of estrogen and DPN in activating astrocytes was lost in Ndrg2 knockout OVX mice and primary cultured astrocytes, but partially retained in conditional knockdown OVX mice. Most importantly, we found that the neuroprotective effects of E2 and DPN against cerebral ischemic injury were lost in Ndrg2 knockout OVX mice but partially retained in conditional knockdown OVX mice. These findings demonstrate that estrogen alleviated cerebral ischemic injury via ERβ upregulation of Ndrg2, which could activate astrocytes, indicating that Ndrg2 is a critical mediator of E2-induced neuroprotection against cerebral ischemic injury.
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http://dx.doi.org/10.1007/s12035-022-02877-5DOI Listing
May 2022
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