Publications by authors named "Jin Wang"

3,498 Publications

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Current Advancements in Animal Models of Postsurgical Lymphedema: A Systematic Review.

Adv Wound Care (New Rochelle) 2021 Jun 15. Epub 2021 Jun 15.

University of Southern California Keck School of Medicine, 12223, Division of Plastic and Reconstructive Surgery, Los Angeles, California, United States.

Significance: Secondary lymphedema is a debilitating disease caused by lymphatic dysfunction characterized by chronic swelling, dysregulated inflammation, disfigurement, and compromised wound healing. Since there is no effective cure, animal model systems that support basic science research into the mechanisms of secondary lymphedema are critical to advancing the field. Recent Advances: Over the last decade, lymphatic research has led to the improvement of existing animal lymphedema models and the establishment of new models. Although an ideal model does not exist, it is important to consider the strengths and limitations of currently available options. In a systematic review adhering to the PRISMA guidelines, we present recent developments in the field of animal lymphedema models and provide a concise comparison of ease, cost, reliability, and clinical translatability.

Critical Issues: The incidence of secondary lymphedema is increasing, and there is no gold standard of treatment or cure for secondary lymphedema.

Future Directions: As we iterate and create animal models that more closely characterize human lymphedema, we can achieve a deeper understanding of the pathophysiology and potentially develop effective therapeutics for patients.
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http://dx.doi.org/10.1089/wound.2021.0033DOI Listing
June 2021

ZnO Nanoneedle-Modified PEEK Fiber Felt for Improving Anti-fouling Performance of Oil/Water Separation.

Langmuir 2021 Jun 14. Epub 2021 Jun 14.

National and Local Joint Engineering Laboratory for Synthetic Technology of High Performance Polymer, College of Chemistry, Jilin University, Changchun 130012, China.

Membrane separation has been considered to be the most effective decontamination method for oily waste water. The most significant point of membrane separation is the resistance against membrane fouling. Fabricating hierarchical architectures on the membrane surface is an available approach to improving its anti-fouling property. In this study, ZnO nanoneedles were successfully anchored onto surface-sulfonated poly(ether-ether-ketone) (PEEK) felt via UV/ozone cleaning and hydrothermal synthesis. The modified felt (PEEK-f-Z) showed much better anti-fouling properties and far higher rejection height (33 cm) than the unmodified felt (17 cm) with a separation efficiency up to 99.99%. The enhanced separation properties could be attributed to the stronger water locking capability of the hierarchical architectures on the surface. Furthermore, benefiting from the great chemical stability of PEEK substrates and ZnO nanoneedles, the as-prepared membrane exhibited admirable solvent resistance, mechanical strength, and thermal stability. As a result, PEEK-f-Z could even separate immiscible organic liquids with different polarities and collect hot water from the oil/water mixture, promising to be used under severe conditions.
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http://dx.doi.org/10.1021/acs.langmuir.1c00838DOI Listing
June 2021

In situ implanting single tungsten site into defective UiO-66(Zr) by solvent-free route for efficient oxidative desulfurization at room temperature.

Angew Chem Int Ed Engl 2021 Jun 13. Epub 2021 Jun 13.

Tsinghua University, Department of Chemistry, CHINA.

Design of single site catalysts with catalytic sites at atomic-scale and high atom utilization, provides new opportunities to gain superior catalytic performance for targeted reactions. In this contribution, we report a one-pot green approach for in-situ implanting single tungsten site (up to 12.7 wt.%) onto the nodes of defective UiO-66(Zr) structure via forming Zr-O-W bonds under solvent-free condition. The catalysts displayed extraordinary activity for the oxidative removal of sulfur compounds (1000 ppm S) at room temperature within 30 min. The turnover frequency (TOF) value can reach 44.0 h -1 at 30°C, which is 109.0, 12.3 and 1.2 times higher than that of pristine UiO-66(Zr), WO 3 , and WCl 6 (homogeneous catalyst). Theoretical and experimental studies show that the anchored W sites can react with oxidant readily and generate W(VI)-peroxo intermediates that determine the reaction activity. Our work not only manifests the application of SSCs in the field of desulfurization of fuel oil but also opens a new solvent-free avenue for fabricating MOFs based SSCs.
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http://dx.doi.org/10.1002/anie.202107018DOI Listing
June 2021

Unifying deterministic and stochastic ecological dynamics via a landscape-flux approach.

Proc Natl Acad Sci U S A 2021 Jun;118(24)

Department of Chemistry, Physics and Applied Mathematics, State University of New York at Stony Brook, Stony Brook, NY 11794-3400

The frequency distributions can characterize the population-potential landscape related to the stability of ecological states. We illustrate the practical utility of this approach by analyzing a forest-savanna model. Savanna and forest states coexist under certain conditions, consistent with past theoretical work and empirical observations. However, a grassland state, unseen in the corresponding deterministic model, emerges as an alternative quasi-stable state under fluctuations, providing a theoretical basis for the appearance of widespread grasslands in some empirical analyses. The ecological dynamics are determined by both the population-potential landscape gradient and the steady-state probability flux. The flux quantifies the net input/output to the ecological system and therefore the degree of nonequilibriumness. Landscape and flux together determine the transitions between stable states characterized by dominant paths and switching rates. The intrinsic potential landscape admits a Lyapunov function, which provides a quantitative measure of global stability. We find that the average flux, entropy production rate, and free energy have significant changes near bifurcations under both finite and zero fluctuation. These may provide both dynamical and thermodynamic origins of the bifurcations. We identified the variances in observed frequency time traces, fluctuations, and time irreversibility as kinematic measures for bifurcations. This framework opens the way to characterize ecological systems globally, to uncover how they change among states, and to quantify the emergence of quasi-stable states under stochastic fluctuations.
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http://dx.doi.org/10.1073/pnas.2103779118DOI Listing
June 2021

miR‑200b upregulation promotes migration of BEAS‑2B cells following long‑term exposure to cigarette smoke by targeting ETS1.

Mol Med Rep 2021 Aug 10;24(2). Epub 2021 Jun 10.

Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China.

Cigarette smoking is the leading cause of all histological types of lung cancer, and the role that microRNAs (miRNAs) serve in its pathogenesis is being increasingly recognized. The aim of the present study was to investigate the role of miR‑200b on migration in cigarette smoke‑induced malignant transformed cells. In the present study, miR‑200b expression was found to be increased in cigarette smoke (CS)‑exposed BEAS‑2B cells, lung cancer cell lines and tumor tissue samples. Using wound healing and Transwell migration assays, the migratory ability was shown to be increased in miR‑200b‑overexpressing cells, whereas miR‑200b knockdown resulted in reduced migration. Additionally, the expression of E‑Cadherin was downregulated, whereas that of N‑Cadherin was upregulated in miR‑200b mimic‑transfected cells, suggesting an increase in epithelial‑mesenchymal transition. Downstream, using four target gene prediction tools, six target genes of miR‑200b were predicted, amongst which, ETS proto‑oncogene 1 transcription factor () was shown to be significantly associated with tumor invasion depth and negatively associated with miR‑200b expression. The interaction between miR‑200b and was confirmed using a dual‑luciferase reporter assay. Using rescue experiments, the increased migratory ability of the miR‑200b‑overexpressing cells was reversed by overexpression. In summary, this study showed that miR‑200b overexpression serves a carcinogenic role and promotes the migration of BEAS‑2B cells following long‑term exposure to CS by targeting .
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http://dx.doi.org/10.3892/mmr.2021.12201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201442PMC
August 2021

[Therapeutic Effect of Spleen Low Molecular Weight Extracts on Leukopenia Caused by Epirubicin in Mice and Its Mechanism].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):969-974

Department of Immunology, School of Basic Medicine, Peking University, Beijing 100191,

Objective: To investigate the therapeutic effect of spleen low molecular weight extracts on epileptics hydrochloride-induced leukopenia in mice and explore its mechanism.

Methods: The model of leukopenia in mice was established by the injection of epirubicin hydrochloride (10 mg/kg). After the injection of chemotherapeutic drugs, leukocytopenia mice were treated with different doses of spleen low molecular weight extract, Ganoderma oral solution and recombinant granulocyte colony stimulating factor (rhG-CSF). The general survival status indicators such as body weight, coat color and athletic ability of mice in each group were recorded; the tail vein blood of mice in each group was collected and the white blood cell count in them was calculated; bone marrow of mice was taken and bone marrow smears were observed.

Results: In the model group, the weight of the mice gradually decreased in the later period, their coat became dark and rough, and the ability to exercise decreased, while the mice in the treatment groups showed different degrees of improvement in their survival status except for the mice treated by rhG-CSF. There was no significant fluctuation in the white blood cell count of the blank control mice. After injection of epirubicin, the white blood cell count of peripheral blood in the model mice and treated mice were decreased. The white blood cell count was lower in the mice treated with high-dose low molecular weight extract and rhG-CSF than that in other experimental groups. Bone marrow smear showed that the proportion of bone marrow nucleated cells in the mice treated with the low molecular weight extract of the spleen was significantly higher than that of model mice (P<0.05).

Conclusion: The low molecular weight spleen extracts can significantly improve the hematopoietic state of mouse bone marrow, promote the proliferation of inhibited bone marrow cells, and thus has the effect of treating leukopenia in mice.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.03.050DOI Listing
June 2021

MicroRNA miR-29a Inhibits Colon Cancer Progression by Downregulating B7-H3 Expression: Potential Molecular Targets for Colon Cancer Therapy.

Mol Biotechnol 2021 Jun 7. Epub 2021 Jun 7.

Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.

MiR-29a belongs to one of the subtypes of miRNAs known as non-coding single-stranded RNAs and is preferentially expressed in normal tissues. B7-H3, a member of the B7/CD28 immunoglobulin superfamily, was shown to be overexpressed in several solid malignant tumors, including colon cancer. In addition, it is associated with tumor progression and poor prognosis. We used immunohistochemical and Western blotting to assess B7-H3 protein expression levels in colon cancer and adjacent normal tissues and then compared their relationships with clinicopathological factors. Quantitative real-time reverse-transcription PCR was used to assess B7-H3 and miRNA-29a mRNA expression levels, and then their relationship and clinical significance were evaluated. In addition, colon cancer Caco-2 cells, which constitutively overexpress B7-H3, were transfected with lentivirus particles for miR-29a upregulation. Invasion and migration assays were carried out in vitro along with the establishment of a subcutaneous xenograft model in vivo to determine the role of miRNA-29a in colon cancer progression. The B7-H3 protein showed elevated expression in colon carcinoma and was relevant to TNM staging, lymph node metastasis, and reduced survival. Meanwhile, miR-29a was preferentially expressed in normal colon tissues, while B7-H3 transcript levels had no marked differences between tumor and normal tissue specimens. In vitro, miR-29a upregulation resulted in reduced B7-H3 expression. Furthermore, miR-29a upregulation reduced the invasive and migratory abilities of colon carcinoma cells. In animal models, upregulation of miR-29a slowed down the growth of subcutaneous xenotransplanted tumors and resulted in prolonged survival time. MiR-29a downregulates B7-H3 expression and accordingly inhibits colon cancer progression, invasion, and migration, indicating miR-29a and B7-H3 might represent novel molecular targets for advanced immunotherapy in colon cancer.
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http://dx.doi.org/10.1007/s12033-021-00348-1DOI Listing
June 2021

Retraction: Chemical synthesis and antigenic activity of a phosphatidylinositol mannoside epitope from .

Chem Commun (Camb) 2021 Jun;57(48):5985

School of Pharmacy, Yancheng Teachers University, Hope Avenue South Road No. 2, Yancheng, Jiangsu Province, P. R. China. and Université de Toulouse, Université Toulouse III - Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 9, France and CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, 31077 Toulouse, France.

Retraction of 'Chemical synthesis and antigenic activity of a phosphatidylinositol mannoside epitope from Mycobacterium tuberculosis' by Shi-Yuan Zhao et al., Chem. Commun., 2020, 56, 14067-14070, DOI: 10.1039/D0CC05573E.
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http://dx.doi.org/10.1039/d1cc90195hDOI Listing
June 2021

Corrigendum: Searching for the Mechanisms of Mammalian Cellular Aging Through Underlying Gene Regulatory Networks.

Front Genet 2021 20;12:684415. Epub 2021 May 20.

Department of Chemistry and Physics, State University of New York at Stony Brook, Stony Brook, NY, United States.

[This corrects the article DOI: 10.3389/fgene.2020.00593.].
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http://dx.doi.org/10.3389/fgene.2021.684415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176301PMC
May 2021

Regulatory Focus, Motivation, and Their Relationship With Creativity Among Adolescents.

Front Psychol 2021 20;12:666071. Epub 2021 May 20.

Beijing Key Laboratory of Learning and Cognition, School of Psychology, Capital Normal University, Beijing, China.

Due to the close relationship among intrinsic/extrinsic motivation, regulatory focus, and creativity revealed by previous literature, intrinsic/extrinsic motivation may play a mediating role between regulatory focus and creativity. Therefore, the present study aimed to investigate the relationship between regulatory focus and creativity by combining intrinsic/extrinsic motivation. In this study, senior high school students ( = 418) completed the Regulatory Focus Questionnaire, the Working Preference Inventory, the Williams Creativity Assessment Packet, and the Kirton Adaption-Innovation Inventory. The correlation analysis showed that both promotion and prevention focus positively correlated with intrinsic motivation; intrinsic motivation and promotion focus positively correlated with creativity personality and innovative-adaptive cognitive style; and extrinsic motivation and prevention focus negatively correlated with innovative-adaptive cognitive style. Furthermore, a path model showed that promotion focus positively predicted creativity through the mediation of intrinsic motivation. In general, our study suggests that intrinsic motivation plays a mediating role between promotion focus and creativity. Our results complement those of previous studies and serve as inspiration for the cultivation of creativity in classroom or enterprise settings.
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http://dx.doi.org/10.3389/fpsyg.2021.666071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172616PMC
May 2021

IL-1β pre-stimulation enhances the therapeutic effects of endometrial regenerative cells on experimental colitis.

Stem Cell Res Ther 2021 Jun 5;12(1):324. Epub 2021 Jun 5.

Department of General Surgery, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China.

Background: Ulcerative colitis (UC) is a chronic, relapsing, and non-specific inflammatory bowel disease, and the current treatment strategies were mainly used to relieve symptoms or for maintenance. Endometrial regenerative cells (ERCs) are mesenchymal-like stromal cells and have been demonstrated to alleviate multiple immune-dysregulation diseases. Pro-inflammatory stimuli were reported to enhance the immunosuppressive functions of ERCs, but the mechanism underlined is not fully understood. Here, we have designed this study to investigate the therapeutic effects of IL-1β-primed ERCs in the attenuation of experimental colitis.

Methods: BALB/c mice were given 3% dextran sodium sulfate (DSS) for 7 consecutive days and free tap water for 3 days sequentially to induce experimental colitis. PBS (200 μL), ERCs, and IL-1β-primed ERCs (10ng/mL, 48 h) were injected (1 million/mouse/day, i.v.) on day 2, 5, and 8, respectively. Colonic and splenic samples were harvested on day 10 after DSS induction.

Results: It was found that IL-1β-primed ERC treatment markedly attenuated colonic damage, body weight loss, and colon length shortening in colitis mice. Compared with other treatments, cell populations of CD4IL-4Th2 cells, CD4CD25FOXP3 regulatory T cells (Tregs), and CD68CD206 macrophages in spleens were also significantly upregulated in the IL-1β-primed ERC-treated group (p < 0.05). In addition, lower expression of pro-inflammatory (IFN-γ, IL-17, TNF-α, and IL-6), but higher levels of anti-inflammatory cytokines (IL-4 and IL-10) were detected in colons in the IL-1β-primed ERC-treated group (p < 0.05 vs. other groups). Importantly, we also found that different generations of ERCs had an overall lower secretion of Dickkopf-1 (DKK1) by IL-1β pre-stimulation (p < 0.05) and a higher expression of β-catenin in colonic and splenic tissues after the administration of IL-1β-primed ERCs.

Conclusions: This study has demonstrated that IL-1β pre-stimulation effectively downregulated DKK1 expression in ERCs, which in turn promoted the wnt/β-catenin pathway activation in colonic and splenic tissues. Consequently, IL-1β-primed ERCs exhibited an enhanced therapeutic effect in the attenuation of DSS-induced colitis.
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http://dx.doi.org/10.1186/s13287-021-02392-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180147PMC
June 2021

New fluctuation theorems on Maxwell's demon.

Authors:
Qian Zeng Jin Wang

Sci Adv 2021 Jun 4;7(23). Epub 2021 Jun 4.

Departments of Chemistry and of Physics and Astronomy, State University of New York, Stony Brook, NY 11794-3400, USA.

With increasing interest in the control of systems at the nano- and mesoscopic scales, studies have been focused on the limit of the energy dissipation in an open system by refining the concept of the Maxwell's demon. To uncover the underlying physical principle behind a system controlled by a demon, we prove a previously unexplored set of fluctuation theorems. These fluctuation theorems imply that there exists an intrinsic nonequilibrium state of the system, led by the nonnegative demon-induced dissipative information. A consequence of this analysis is that the bounds of both work and heat are tighter than the limits predicted by the Sagawa-Ueda theorem. We also suggest a possible experimental test of these work and heat bounds.
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http://dx.doi.org/10.1126/sciadv.abf1807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177699PMC
June 2021

Induced pluripotent stem cells derived from one 70-years-old male donor with the APOE-ε4/ε4 alleles.

Stem Cell Res 2021 May 13;53:102395. Epub 2021 May 13.

Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China. Electronic address:

Apolipoprotein E (ApoE) is a lipid-binding protein with ε2, ε3, and ε4 allelic variants in human. The ε4 isoform (ApoE4) is the strongest genetic risk factor for the late-onset form of Alzheimer's disease (AD), and is also associated with multiple neurological disorders, multiple sclerosis, and cerebrovascular disease. Here, induced pluripotent stem cells were derived from the peripheral blood mononuclear cells of a 70-year-old male donor with APOE-ε4/ε4 alleles background to explore pathogenesis and screen potential treatment methods in neurodegenerative diseases. In the newly-developed induced pluripotent stem cell line, the pluripotent markers were well expressed. In addition, the generated cells displayed a normal karyotype and have differentiation potential.
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http://dx.doi.org/10.1016/j.scr.2021.102395DOI Listing
May 2021

Correction: An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors.

Analyst 2021 Jun 4. Epub 2021 Jun 4.

Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, People's Republic of China. and School of Nano Technology and Nano Bionics, University of Science and Technology of China, Hefei, 230026, People's Republic of China.

Correction for 'An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors' by Jin Wang et al., Analyst, 2020, 145, 2725-2730, DOI: 10.1039/C9AN01809C.
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http://dx.doi.org/10.1039/d1an90048jDOI Listing
June 2021

Diagnostic accuracy of 3D magnetic resonance elastography for assessing histologic grade of hepatocellular carcinoma: comparison of three methods for positioning region of interest.

Abdom Radiol (NY) 2021 Jun 3. Epub 2021 Jun 3.

Department of Radiology, The Third Affiliated Hospital, Sun Yat-Sen University (SYSU), Tianhe Road, No 600, Guangzhou, Guangdong, 510630, People's Republic Of China.

Purpose: To assess the influence of region of interest (ROI) placement on the predictive value of 3D MRE in differentiating the histologic grade of HCC.

Methods: 85 patients with pathologically confirmed HCCs were analyzed using 3D MRE imaging, two radiologists measured the tumor stiffness with three different ROI positioning methods. Intraclass correlation coefficient (ICC) was expressed in terms of inter- and intra-observer agreements. Kruskal-Wallis rank test or one-way ANOVA was used to compare the difference in MRE stiffness across the three-ROI positioning methods. Receiver operating characteristic curve analysis (ROC) was performed, and the area under curve (AUC) was measured to evaluate the diagnostic performance.

Results: There were 64 (75%) well-or-moderately differentiated HCCs and 21(25%) poorly differentiated HCCs included finally. Almost excellent inter- and intra-observer agreements (all ICC > 0.82) were observed for all three-ROI methods, the volumetric method has the highest values (inter-observer ICC 0.967, intra-observer ICC 0.919, 0.926, respectively). The mean stiffnesses of poorly differentiated HCC obtained by two readers were significantly higher than well-or-moderately differentiated HCC with volumetric method (7.07 ± 1.57 Kpa, 5.00 ± 1.49 Kpa, and 6.85 ± 1.49 Kpa, 4.94 ± 1.48 Kpa, respectively) and three-ROI method (6.14 ± 1.71 Kpa, 4.91 ± 1.56 Kpa and 5.94 ± 1.61 Kpa, 4.84 ± 1.54 Kpa, respectively) but not on single-ROI method (p > 0.005), for the diagnostic performance, the highest area under the curve (AUC) with a value of 0.837, 0.812 by using the volumetric method, followed by the three-ROI method (0.713, 0.754) and single-ROI method.

Conclusion: Different ROI positioning methods significantly affect HCC tumor stiffness measurements. The whole tumor volumetric analysis is superior to ROI-based methods for predicting the grade of HCC.
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http://dx.doi.org/10.1007/s00261-021-03150-4DOI Listing
June 2021

Manganese (II) chloride leads to dopaminergic neurotoxicity by promoting mitophagy through BNIP3-mediated oxidative stress in SH-SY5Y cells.

Cell Mol Biol Lett 2021 Jun 2;26(1):23. Epub 2021 Jun 2.

Department of Neurology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.

Background: Manganese overexposure can induce neurotoxicity, lead to manganism and result in clinical manifestations similar to those of parkinsonism. However, the underlying molecular mechanism is still unclear. This study demonstrated that MnCl induces mitophagy and leads to neurotoxicity by promoting BNIP3-mediated reactive oxygen species (ROS) generation.

Methods: Human neuroblastoma SH-SY5Y cells were used throughout our experiments. Cell viability was detected by cell proliferation/toxicity test kits. Mitochondrial membrane potential was measured by flow cytometry. ROS generation was detected using a microplate reader. Protein levels were evaluated by Western blot. Transmission electron microscopy was used to evaluate mitochondrial morphology. Co-immunoprecipitation was used to verify the interaction between BNIP3 and LC3.

Results: MnCl led to loss of mitochondrial membrane potential and apoptosis of SH-SY5Y cells by enhancing expression of BNIP3 and conversion of LC3-I to LC3-II. Moreover, MnCl reduced expression of the mitochondrial marker protein TOMM20 and promoted interaction between BNIP3 and LC3. The results also indicated that a decrease in BNIP3 expression reduced the mitochondrial membrane potential loss, attenuated apoptosis and reduced mitochondrial autophagosome formation in SH-SY5Y cells after MnCl treatment. Finally, we found that manganese-induced ROS generation could be reversed by the antioxidant N-acetyl cysteine (NAC) or silencing BNIP3 expression.

Conclusions: BNIP3 mediates MnCl-induced mitophagy and neurotoxicity in dopaminergic SH-SY5Y cells through ROS. Thus, BNIP3 contributes to manganese-induced neurotoxicity by functioning as a mitophagy receptor protein.
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http://dx.doi.org/10.1186/s11658-021-00267-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173824PMC
June 2021

Micro-Structure Changes Caused by Thermal Evolution in Chalcogenide GeAsSe Thin Films by In Situ Measurements.

Materials (Basel) 2021 May 15;14(10). Epub 2021 May 15.

College of Applied Sciences, Beijing University of Technology, Beijing 100124, China.

To understand the effects of thermal annealing on the structure of GeAsSe thin films, the thermal evolution of these films was measured by the in situ X-ray diffraction (XRD) at different temperature (773 K or 1073 K) in a vacuum (10 Pa) environment. The entire process of crystallization can be observed by using in situ XRD, which is from the appearance of a crystal structure to melting liquid-state and ultimately to the disappearance of the amorphous structure. In the crystallized process, the corresponding state-transition temperatures T (the onset crystallization temperature), T (the transition temperature from glassy-state to liquid-state), T (peak crystallization temperature) are linear with MCN (Mean Coordination Number). In order to obtain information about changes in the amorphous structural origin of the anneal-induced material, the samples were analyzed by in situ Raman spectroscopy. Analysis of the results through decomposing the Raman spectra into different structural units showed that the Ge-Ge, As-As, or Se-Se homopolar bonds as the nonequilibrium minority carriers could be found in films. It suggests that the formation of these bonds cannot be completely suppressed in any case, as one falls and another rises.
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http://dx.doi.org/10.3390/ma14102572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156157PMC
May 2021

Modified Natural Dolomite and Its Influence on the Production of Glycerol Carbonate: Effects of Structural and Basicity Properties.

Materials (Basel) 2021 May 1;14(9). Epub 2021 May 1.

Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey, Nuevo León 64849, Mexico.

A systematic study over different treatment conditions, including hydrothermal and acid-thermal, was successfully carried out to determine the most suitable conditions to enhance the textural properties and surface chemical composition of natural dolomite. The reconstruction of dolomite after various treatments enhanced the surface area by 4-5 times and diminished the pore diameter between 70% and 81% compared to the untreated parent dolomite. The Rietveld analysis of the X-ray diffraction (XRD) patterns revealed changes in the crystalline compositions after each treatment. When the treated dolomite was used as a catalyst to produce glycerol carbonate via a transesterification reaction of glycerol and dimethyl carbonate, the crystalline Ca(OH) concentration of the modified dolomite and the apparent glycerol carbonate formation rate (r) are well-correlated. The results suggest that an increase of the crystalline Ca(OH) concentration could be related with surface basicity at the weak and moderate strength sites that may lead to an increase in catalytic activity. The hydrothermal treated dolomite showed a selectivity of glycerol carbonate greater than 99% and r value 3.42 mmol/min·g, which was higher than that achieved on other samples. This study could aid to the proper selection of dolomite treatment for the desired crystalline composition, depending on the applications of this highly available mineral.
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http://dx.doi.org/10.3390/ma14092358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125416PMC
May 2021

The Stratified Distribution of Dissolved Organic Matter in an AMD Lake Revealed by Multi-sample Evaluation Procedure.

Environ Sci Technol 2021 Jun 1;55(12):8401-8409. Epub 2021 Jun 1.

School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.

As a typical extreme environment, acid mine drainage (AMD) has been extensively studied for its biogeochemical cycle, but little is known about the quality of dissolved organic matter (DOM) in AMD. In this study, DOM molecules in an AMD lake were detected with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), and the change of DOM molecules in the stratified water column was analyzed with a multi-sample evaluation procedure. The results demonstrate that DOM quality is highly stratified and can be linked with severe biogeochemical gradients. In the surface layer, DOM molecules can be distinguished by low quantities and intensities, as well as potential photodegradation products. Oxygen-poor and oxygen-rich molecules alternately dominate the chemocline, which can be explained by the redox-dependent adsorption/desorption of DOM on metastable secondary minerals. A rich and abundant DOM pool with a high proportion of heteroatoms exists at the bottom which can be significantly influenced by material exchange with sediments. These findings emphasize the active role of DOM in extreme AMD environments and expand the understanding of the carbon cycle in the hydrosphere.
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http://dx.doi.org/10.1021/acs.est.0c05319DOI Listing
June 2021

Galla Chinensis, a Traditional Chinese Medicine: Comprehensive review of botany, traditional uses, chemical composition, pharmacology and toxicology.

J Ethnopharmacol 2021 May 27;278:114247. Epub 2021 May 27.

State Key Laboratory of Southwestern Chinese Medicine Resources, No.1166, Liutai Road, Wenjiang District, Chengdu, 611137, China. Electronic address:

Ethnopharmacological Relevance: Galla chinensis (GC), a traditional Chinese medicine (TCM), has a wide range of pharmacological properties which have been widely used for more than 1400 years. Based on shape, GC is divided into two groups: jiaobei and dubei. It is a bitter, sour, cold and astringent substance which is usually used for treating diarrhea, constipation, bleeding, cough, vomiting, sweating, hemorrhoids, and anal and uterine prolapse. It is distributed in Japan, North Korea, and all parts of China.

Aim Of Study: This study was aimed at carrying out a comprehensive overview of the current status of research on Galla chinensis (GC) for better understanding of it characteristics, while providing a clear direction for future studies. It has aroused the interest of researchers, leading to development of medicinal value, expansion of its application, and provision of wider and more effective drug choices. This study was focused on the traditional uses, botany, chemical composition, pharmacology and toxicology of GC. Finally, the study focused on possible future research directions for GC.

Materials And Methods: A comprehensive analysis was done based on academic papers, pharmaceutical monographs, ancient medicinal works, and drug standards of China. This review used Galla and Galla chinensis as keywords for retrieval of information on GC from online databases such as PubMed, Elsevier, CNKI, Web of Science, Google Scholar, SCI hub, and Baidu academic.

Results: It was found that the chemical constituents of GC included tannins, phenolic acid, amino acids and fatty acid, with polyphenol compounds (especially tannins and gallic acid) as the distinct components. In vitro and in vivo studies revealed that GC exerted numerous biological effects such as anti-caries, antibacterial, antiviral, anticancer, and antioxidant effects. The therapeutic effect of GC was attributed mainly to the biological properties of its bioactive components.

Conclusions: GC is an important TCM which has potential benefit in the treatment of a variety of diseases. However, the relationship amongst the structure and biological activity of GC and its components, mechanism of action, toxicity, pharmacokinetics and target organs need to be further studied. Quality control and quality assurance programs for GC need to be further developed. There is need to study the dynamics associated with the accumulation of chemical compounds in GC as well as the original plants and aphid that form GC.
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http://dx.doi.org/10.1016/j.jep.2021.114247DOI Listing
May 2021

Accurate nucleic acid quantification in a single sample tube without the need for calibration.

Anal Chim Acta 2021 Jul 3;1167:338599. Epub 2021 May 3.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, Xiamen, 361102, China; School of Life Sciences, Xiamen University, Xiamen, 361102, China.

Convenient and accurate nucleic acid quantification (NAQ) is crucial to clinical diagnosis, forensic medicine, veterinary medicine and food analysis. However, traditional NAQ relies on the preparation of a laborious, time-consuming and expensive calibration curve, which would also propagate pipette errors through serially dilutions. Besides, traditional NAQ is run in different tubes, which introduces bias from random tube-to-tube variations and is unable to detect inhibitors from biological samples. To solve these problems, a single-tube quantitative PCR (stqPCR) technique is proposed which enables accurate quantification without the need for a calibration curve. In this method, an internal quantitative standard DNA (IQS-DNA) for quantification was screened out by co-amplification with the target DNA. Then the difference between the quantification cycle value (ΔCq) of the IQS-DNA and the target DNA was used for NAQ. The method permitted high accuracy quantification with reliable data for concentrations in plasmid, serum standard, and clinical samples being confirmed (R values of 0.9951, 0.9889, and 0.9727, slope values of 1.011, 1.028, and 0.9327, and intercept values of -0.06037, -0.1486, and 0.3325, respectively). Accurate NAQ could also be achieved by stqPCR even though inhibitors were present in a sample; however, in the case of using a commercial assay kit, satisfactory performance was only attained after the same sample was diluted some 32-fold. Moreover, integration of the present method into a microfluidic system could achieve super-fast NAQ in less than 30 min and achieve super-fast "sample in, quantitative answer out" testing in less than 40 min. Thus, the stqPCR method present here would promote the development of NAQ in the laboratory and on site.
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http://dx.doi.org/10.1016/j.aca.2021.338599DOI Listing
July 2021

A Partial Discharge Study of Medium-Voltage Motor Winding Insulation Under Two-Level Voltage Pulses With High Dv/Dt.

IEEE Open J Power Electron 2021 Mar;2:225-235

Department of Electrical and Computer Engineering, The Ohio State University, Columbus, OH 43210 USA.

Medium-voltage (e.g., 10 kV rated) silicon carbide (SiC) devices have great potentials in medium-voltage variable speed drives. But their high switching dv/dt can increase the voltage stress on motor windings and cause partial discharges. This paper presents a partial discharge study of a medium-voltage form-wound winding under two-level square-wave voltage pulses. A 10 kV SiC device-based test platform is built to generate voltage pulses with high dv/dt. A three-step test approach is proposed and employed to systematically investigate the effects of various voltage parameters on partial discharges. These include voltage rise/fall time, voltage pulse width, pulse repetitive rate, duty ratio, voltage polarity, fundamental frequency, and modulation index. Partial discharge inception voltages (PDIVs) and repetitive partial discharge inception voltages (RPDIVs) of the sample are measured with varied voltage parameters. Test results show that voltage rise/fall time is a major affecting factor which reduces PDIVs of the winding sample by 6.5% when it decreases from 800 ns to 100 ns. Based on test results, a hypothetical partial discharge mechanism is presented to explain the effects of fast voltage rise/fall edges. An empirical equation is also derived to estimate PDIVs of a winding sample under various voltage rise/fall time and pulse width conditions.
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http://dx.doi.org/10.1109/ojpel.2021.3069780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152218PMC
March 2021

Efficacy and safety of short-wave diathermy treatment for moderate COVID-19 patients: a prospective, double-blind, randomized controlled clinical study.

Eur J Phys Rehabil Med 2021 May 27. Epub 2021 May 27.

Department of Rehabilitation Medicine, Xijing Hospital, the Fourth Military Medical University, Xi'an, China -

Background: Millions of human beings have suffered in the epidemic of coronavirus disease 2019 (COVID-19), but until now the effective treatment methods have been limited.

Aim: This study aimed to evaluate the efficacy and safety of short-wave diathermy (SWD) treatment for moderate COVID-19 patients.

Design: A prospective, double-blind, randomized controlled clinical study.

Setting: Inpatients unit of a COVID-19 specialized hospital.

Population: Forty-two patients with moderate COVID-19 were randomly allocated at a 2:1 ratio to two groups: the SWD group and the control group.

Methods: Participants of the SWD group received SWD treatment, and participants of the control group received placebo SWD treatment for one session per day, 10 minutes per session, for no more than 14 days. Both groups were given standard care treatment. Primary outcome was the rate of clinical improvement according to a sevencategory ordinal scale. Secondary outcomes included the rate of computed tomography (CT) improvement and the rate of potential adverse events.

Results: Clinical improvement occurred in 92.6% of patients in the SWD group by day 14 compared with 69.2% of patients in the control group (P = 0.001). The Cox model indicated that the SWD group had a higher clinical improvement probability than the control group (Hazard Ratio, 3.045; 95% CI, 1.391-6.666; P = 0.005). Similarly, CT improvement occurred in 85.2 % of patients in the SWD group and 46.2 % of patients in the control group respectively by day 14 (P = 0.001). The Cox model indicated SWD group had a higher CT improvement probability than control group (Hazard Ratio, 3.720; 95% CI, 1.486-9.311; P = 0.005). There was no significant difference in adverse events between the SWD group and the control group (2 of 27 [7.4%] SWD vs. 1 of 13 [7.7%] control, P = 1.000), the most frequent of which were headache (1 of 27 [3.7%] SWD vs. 1 of 13 [7.7%] control patients) and dizziness (1 of 27 [3.7%] SWD vs. 0 of 13 [0%] control patients).

Conclusions: SWD is a valid and reliable adjuvant therapy with a favorable safety profile for moderate COVID-19 patients.

Clinical Rehabilitation Impact: Clinically relevant information is lacking regarding the efficacy and safety of SWD for patients with COVID-19. This study provides the first evidence that SWD is a promising adjuvant therapy for COVID-19.
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http://dx.doi.org/10.23736/S1973-9087.21.06892-1DOI Listing
May 2021

Theoretical investigations on the charge transport properties of anthracene derivatives with aryl substituents at the 2,6-position-thermally stable "herringbone" stacking motifs.

Phys Chem Chem Phys 2021 Jun;23(22):12679-12691

School of Physics, Northeast Normal University, Changchun, 130024, China.

High-performance organic semiconductor materials based on the small aromatic anthracene-core and its derivatives develop comparatively slowly due to the lack of a profound understanding of the influence of chemical modifications on their charge-transfer properties. Herein, the electronic properties and the charge transport characteristics of several typical anthracene-based derivatives with aryl groups substituted at the 2,6-site are systematically investigated by multi-scale simulation methods including Molecular Dynamics (MD) simulation and the full quantum nuclear tunneling model in the framework of density functional theory (DFT). To elucidate the origin of different charge transport properties of these anthracene-based materials, analysis of the molecular stacking and noncovalent intermolecular interaction caused by different substituents was carried out. The results indicate that the electron and hole injection capabilities and the air oxidation stability of the anthracene derivatives are greatly improved when the size of the aryl substituent increases. In addition, the incorporation of 2,6-site aryl substituents can inhibit the stretching vibration of the anthracene-core during charge transport, and allow molecular packing along the long axis (a-axis of DPA and BDBFAnt, and c-axis of dNaAnt) with almost no slippage, and the main transport channels remain unchanged, exhibiting more isotropic 2D transport properties. It should be emphasized that the edge-to-face dimers with smallest dihedral angles are closest to the thermally stable dimer model, with relatively larger π-orbital distributions in transmission channels (dimer 1, 2) and the largest spatial overlap, resulting in the largest hole transfer integral in DPA (Vh1/h2 = 57 meV). Although the analysis of the thermal disorder effect shows a phonon scattering effect, the maximum hole mobility of the DPA molecule is still as high as 1.5 cm2 V-1 s-1.
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http://dx.doi.org/10.1039/d1cp00178gDOI Listing
June 2021

The prevalence and risk factors of acute kidney injury in patients undergoing hip fracture surgery: a meta-analysis.

Bioengineered 2021 Dec;12(1):1976-1985

Department of Orthopaedics, Wuwei People's Hospital, Wuwei, China.

Acute kidney injury (AKI) was a frequent complication following hip fracture surgery, but recent studies reported inconsistent findings. Our study was aimed at clarifying the prevalence and risk factors of AKI after hip fracture surgery. Pubmed, Embase, and Web of Science were systematically searched from the inception to March 2020 to identify observational studies investigating the prevalence and risk factors of AKI in patients undergoing hip fracture surgery. Pooled prevalence and odds ratios (ORs) with 95% confidence intervals (CIs) were estimated using a random-effects model. Publication bias was evaluated with a funnel plot and statistical test. All the statistical analyses were performed using Stata version 12.0. A total of 11 studies with 16,421 patients was included in the current meta-analysis. The pooled prevalence of AKI in patients undergoing hip fracture surgery was 17% (95%CI, 14%-21%) with substantial heterogeneity (I = 95%). Postoperative serum albumin (OR 1.80; 95%CI, 1.38-2.36) was a significant predictor for AKI. Age (OR 1.01; 95%CI, 0.95-1.07) and ACE inhibitors (OR 1.38; 95%CI, 0.92-2.07) were associated with increased the risk of AKI, but the results were not statistically significant. No significant publication bias was identified through statistical tests (Egger's test, = 0.258 and Begg's test, = 0.087). In conclusion, our findings indicated that he pooled AKI following hip fracture surgery was approximately 17%. Postoperative serum albumin was a potential significant risk factor for AKI.
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http://dx.doi.org/10.1080/21655979.2021.1926200DOI Listing
December 2021

Comparison of the diagnostic performance of 2D and 3D MR elastography in staging liver fibrosis.

Eur Radiol 2021 May 22. Epub 2021 May 22.

Department of Radiology, The Third Affiliated Hospital, Sun Yat-Sen University (SYSU), No. 600, Tianhe Road, Guangzhou, Guangdong, 510630, People's Republic of China.

Objectives: To compare the diagnostic performance and image quality of state-of-the-art 2D MR elastography (MRE) and 3D MRE in the basic application of liver fibrosis staging.

Methods: This retrospective study assessed data from 293 patients who underwent 2D and 3D MRE examinations. MRE image quality was assessed with a qualitative 2-point grading system by evaluating artifacts. Two experienced analysts independently measured mean liver stiffness values. The interobserver agreement of liver stiffness measurement was assessed by the intraclass correlation coefficient (ICC). The area under the receiver operating characteristic curve (AUC) was used to assess the diagnostic performance of 2D and 3D MRE and blood-based markers for fibrosis staging using the pathology-proven liver fibrosis stage as the gold standard.

Results: The image quality provided by 3D MRE was graded as significantly higher than that obtained with the 2D MRE method (p < 0.01). Interobserver agreement in liver stiffness measurements was higher for 3D MRE (ICC: 3D 0.979 vs 2D 0.955). The AUC values for discriminating ≥ F1, ≥ F2, ≥ F3, and F4 fibrosis for 3D MRE (0.89, 0.92, 0.95, and 0.93) were similar to those for 2D MRE (0.89, 0.91, 0.94, and 0.92). Both the 2D and 3D MRE methods provided superior accuracy to the blood-based biomarkers, including APRI, FIB-4, and Forns index, especially for ≥ F2, ≥ F3, and F4 fibrosis stages (all p < 0.01).

Conclusions: While 3D MRE offers certain advantages and opportunities for new applications of MRE, current widely deployed 2D MRE technology has comparable performance in the basic application of detecting and staging liver fibrosis.

Key Points: • 2D MRE and 3D MRE have comparable diagnostic performance in detecting and staging liver fibrosis. • 3D MRE has superior image quality and interobserver agreement compared to 2D MRE.
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http://dx.doi.org/10.1007/s00330-021-08053-yDOI Listing
May 2021

The ketogenic diet increases Neuregulin 1 expression via elevating histone acetylation and its anti-seizure effect requires ErbB4 kinase activity.

Cell Biosci 2021 May 21;11(1):93. Epub 2021 May 21.

Emergency Department, Institute of Neuroscience, Department of Neurology of the Second Affiliated Hospital of Guangzhou Medical University, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, Guangzhou, 510260, China.

Background: The ketogenic diet (KD)has been considered an effective treatment for epilepsy, whereas its underlying mechanisms remain obscure. We have previously reported that the KD feeding increased Neuregulin 1 (NRG1) expression in the hippocampus; disruption of NRG1 signaling by genetically deleting its receptor-ErbB4 abolished KD's effects on inhibitory synaptic activity and seizures. However, it is still unclear about the mechanisms underlying the effect of KD on NRG1 expression and whether the effects of KD require ErbB4 kinase activity.

Methods: The effects of the KD on NRG1 expression were assessed via western blotting and real-time PCR. Acetylation level at the Nrg1 promoter locus was examined using the chromatin immunoprecipitation technique. Kainic acid (KA)-induced acute seizure model was utilized to examine the effects of KD and histone deacetylase inhibitor-TSA on seizures. Synaptic activities in the hippocampus were recorded with the technique of electrophysiology. The obligatory role of ErbB4 kinase activity in KD's effects on seizures and inhibitory synaptic activity was evaluated by using ErbB kinase antagonist and transgenic mouse-T796G.

Results: We report that KD specifically increases Type I NRG1 expression in the hippocampus. Using the chromatin immunoprecipitation technique, we observe increased acetylated-histone occupancy at the Nrg1 promoter locus of KD-fed mice. Treatment of TSA dramatically elevates NRG1 expression and diminishes the difference between the effects of the control diet (CD) and KD. These data indicate that KD increases NRG1 expression via up-regulating histone acetylation. Moreover, both pharmacological and genetic inhibitions of ErbB4 kinase activity significantly block the KD's effects on inhibitory synaptic activity and seizure, suggesting an essential role of ErbB4 kinase activity.

Conclusion: These results strengthen our understanding of the role of NRG1/ErbB4 signaling in KD and shed light on novel therapeutic interventions for epilepsy.
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http://dx.doi.org/10.1186/s13578-021-00611-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139023PMC
May 2021

Chlorogenic acid supplementation ameliorates hyperuricemia, relieves renal inflammation, and modulates intestinal homeostasis.

Food Funct 2021 Jun;12(12):5637-5649

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China. and Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.

Hyperuricemia (HUA) is induced by abnormal purine metabolism and elevated serum uric acid (UA) concentrations, and it is often accompanied by inflammatory responses and intestinal disorders. This study aims to assess the protective effects of chlorogenic acid (CGA) on HUA in mice. CGA or allopurinol was given to mice with HUA induced by hypoxanthine and potassium oxonate. CGA lowered the levels of UA, blood urea nitrogen (BUN), creatinine (CR), AST, and ALT; inhibited xanthine oxidase (XOD) activity; and downregulated the mRNA expression of UA secretory proteins in HUA mice. Moreover, CGA significantly reduced serum lipopolysaccharides (LPS) levels and the mRNA expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, NOD-like receptor superfamily pyrin domain containing 3 (NLRP3), and caspase-1, and it inhibited the activation of the toll-like receptor 4/myeloid differentiation factor 88/nuclear factor kappa B (TLR4/MyD88/NF-κB) signaling pathway in the kidney, resulting in inflammation relief in HUA mice. In addition, CGA treatment increased the production of fecal short-chain fatty acids (SCFAs) in HUA mice. Additional investigations showed that CGA significantly lowered the mRNA expression of ileal IL-1β and IL-6, and it increased the mRNA expression of intestinal tight junction proteins (zonula occludens-1 (ZO-1) and occludin). Also, CGA increased the relative abundance of SCFA-producing bacteria, including Bacteroides, Prevotellaceae UGC-001, and Butyricimonas, and it reversed the purine metabolism and glutamate metabolism functions of gut microbiota. In conclusion, CGA may be a potential candidate for relieving the symptoms of HUA and regulating its associated inflammatory responses and intestinal homeostasis.
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http://dx.doi.org/10.1039/d0fo03199bDOI Listing
June 2021

Nursing effects of finger exercise on cognitive function and others for cerebral ischemic stroke patients.

Am J Transl Res 2021 15;13(4):3759-3765. Epub 2021 Apr 15.

Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong University Xi'an, Shaanxi Province, China.

Objective: To investigate the nursing effects of finger exercise training on cognitive function and others for patients with cerebral ischemic stroke (CIS).

Methods: A total of 200 patients with CIS were selected in this prospective study. According to the random number table method, they were divided into control group (n=100, routine nursing) and research group (n=100, routine nursing combined with finger exercise training). Various scales were used to evaluate the cognitive function, hand function, upper limb motor function, wrist flexor muscle tone, degree of neurological impairment and ability of daily living (ADL) in the two groups before and after intervention. And the incidence of mild vascular cognitive impairment (VCI) after intervention was compared.

Results: After intervention, Montreal Cognitive Assessment (MoCA), Mini Mental State Examination (MMSE), hand function, Fugl-meyer Assessment (FMA) and ADL scores in both groups were significantly increased, and those in the research group were significantly higher than those in the control group (all P<0.05). There were opposite trends in the Neurologic Functional Defect (NIHSS) and Modified Ashworth Scale (MAS) for wrist flexor scores (all P<0.05). The incidence of mild VCI in the research group was significantly lower than that in the control group (P<0.05).

Conclusion: On the basis of early rehabilitation nursing, combined finger exercise training can improve cognitive function, hand function, upper limb function and ADL for patients with CIS.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129410PMC
April 2021