Publications by authors named "Jin Liu"

1,760 Publications

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Sources, transformations of suspended particulate organic matter and their linkage with landscape patterns in the urbanized Beiyun river Watershed of Beijing, China.

Sci Total Environ 2021 Jun 5;791:148309. Epub 2021 Jun 5.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, P R China. Electronic address:

This study explored the sources, transformations of suspended particulate organic matter (POM), and the influence of landscape patterns on POM within the Beiyun River Watershed by applying the stable carbon and nitrogen isotope technique combined with multiple statistical analyses. The POM variables showed great spatial fluctuations under different urban development gradients. Analysis of multiple isotopes revealed that assimilation of phytoplankton might exist in the rainy season, while nitrification occurs in the dry season. SIAR modeling results indicated that the sewage debris and phytoplankton were the main sources of POM in both seasons, accounting for 52.58% and 38.39% in the rainy season, 33.17% and 31.95% in the dry season, respectively. Spatiotemporal variations of POM sources existed in the study watershed, probably due to urbanization and human disturbance. The multiple linear stepwise regression and redundant analysis results indicated that landscape metrics reflecting contagion and fragmentation at the class level correlated well with the POM variables over seasons. Interspersion and juxtaposition indices of grassland and water were negatively related to POM variables in the rainy season, whereas the landscape division index of buildup land showed negative correlations with POM parameters in the dry season. Increasing the adjacency of grassland and water to other land uses, while reducing the aggregation of buildup lands would be an efficient way for urban river water quality improvement.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148309DOI Listing
June 2021

Synthesis of MnO/C/CoO nanocomposites by a Mn-oxidizing bacterium as a biotemplate for lithium-ion batteries.

Sci Technol Adv Mater 2021 Jun 4;22(1):429-440. Epub 2021 Jun 4.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.

The biotemplate and bioconversion strategy represents a sustainable and environmentally friendly approach to material manufacturing. In the current study, biogenic manganese oxide aggregates of the Mn-oxidizing bacterium sp. T34 were used as a precursor to synthesize a biocomposite that incorporated Co (CMC-Co) under mild shake-flask conditions based on the biomineralization process of biogenic Mn oxides and the characteristics of metal ion subsidies. X-ray photoelectron spectroscopy, phase composition and fine structure analyses demonstrated that hollow MnO/C/CoO multiphase composites were fabricated after high-temperature annealing of the biocomposites at 800°C. The cycling and rate performance of the prepared anode materials for lithium-ion batteries were compared. Due to the unique hollow structure and multiphasic state, the reversible discharge capacity of CMC-Co remained at 650 mAh g after 50 cycles at a current density of 0.1 Ag, and the coulombic efficiency remained above 99% after the second cycle, indicating a good application potential as an anode material for lithium-ion batteries.
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http://dx.doi.org/10.1080/14686996.2021.1927175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183561PMC
June 2021

Visible-light excitable Eu-induced hyaluronic acid-chitosan aggregates with heterocyclic ligands for sensitive and fast recognition of hazardous ions.

Int J Biol Macromol 2021 Jun 10;184:188-199. Epub 2021 Jun 10.

Institute of Hybrid Materials, National Center of International Joint Research for Hybrid Materials Technology, National Base of International Sci. & Technology Cooperation on Hybrid Materials, Qingdao University, 308 Ningxia Road, Qingdao 266071, China. Electronic address:

Water-soluble luminescent lanthanide complexes that can be excited with visible light could enable rapid detection of toxic anions and cations in biological systems. Eu-induced hyaluronic acid-chitosan aggregates (EIHCA) can improve the stability, biocompatibility, efficiency, and light absorption of luminescent Eu complexes. Visible-range excitation may avoid phototoxicity associated with overexposure to UV light in biological and ecological applications. In this work, we synthesized and characterized series of EIHCA complexes having three N-donor heterocyclic ligands: 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline (Dphen), 2,2': 6',2″-terpyridine (Tpy) and 1,10-phenanthroline monohydrate (Phen). These complexes possessed bright red fluorescence with a visible range excitation maximum. The photophysical properties of one formulation (we denote as EDL6) include fast quenching response (20 s) of the fluorescence, multi-selectivity, low limit of detection, and high quenching (K) values, enabling selective, rapid and sensitive recognition of CrO and Fe in aqueous solution. Furthermore, EDL6 exhibits cytocompatibility with mammalian cells that make these complexes promising biocompatible candidate as a safe replacement of organic fluorophores for fluorescence sensing applications. Thus, these new EIHCA complexes were successfully employed for the selective detection of hazardous materials in biological and aqueous environment samples.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.051DOI Listing
June 2021

Efficacy and safety of ciprofol for the sedation/anesthesia in patients undergoing colonoscopy: Phase IIa and IIb multi-center clinical trials.

Eur J Pharm Sci 2021 Jun 8:105904. Epub 2021 Jun 8.

Department of Anesthesiology, West China Hospital, Sichuan University & The Research Units of West China (2018RU012), Chinese Academy of Medical Sciences Chengdu, China. Electronic address:

Objective: Ciprofol is a new intravenous anesthetic agent similar to propofol that has the pharmacodynamic characteristics of a rapid rate of onset and recovery in pre-clinical experiments. The aims of the present clinical trials were to compare the efficacy and safety of ciprofol emulsion for sedation or general anesthesia during colonoscopy and to define optimal doses for a subsequent phase III clinical trial.

Methods: A phase IIa multi-center, open-label, non-randomized, positive control, dose-escalating study was performed to determine a recommended phase IIb dose (RP2D) of ciprofol to induce sedation or anesthesia in patients undergoing colonoscopy. Phase IIb was also a multi-center clinical trial, but the patients were randomized into 3 groups at a ratio of 1:1:1. It was a double-blinded, propofol controlled study that administered ciprofol 0.4 mg/kg (n = 31) and 0.5 mg/kg (n = 32) or propofol at 2.0 mg/kg (n = 31), with the aim of establishing the optimal dose of ciprofol. The primary endpoint was the colonoscopy success rate. Secondary endpoints were the duration of colonoscope insertion, recovery time, number of top-up doses needed, and the total dose of ciprofol or propofol required to maintain adequate sedation or anesthesia. In addition, we evaluated the satisfaction of sedation/anesthesia from the endoscopists, anesthetists and patients' points of view. Safety was assessed according to the incidence of AEs including serious AEs and drug related AEs and the assessment of vital signs, a 12-lead ECG and laboratory tests.

Results: In the phase IIa trial, the colonoscopy success rates in the 0.2 ∼ 0.5 mg/kg ciprofol and propofol 2.0 mg/kg groups were 100% and all doses were safe and well tolerated. Ciprofol doses of 0.4 mg/kg and 0.5 mg/kg are recommended for subsequent IIb phases. In the phase IIb trial, a 100% success rate was reconfirmed in all the dosage groups. The mean time of colonoscope insertion in the ciprofol 0.4 mg/kg, ciprofol 0.5 mg/kg and propofol 2.0 mg/kg groups were 1.9, 1.5 and 1.5 min, the mean recovery times from colonoscope withdrawal were 6.1, 5.1, and 4.3 min, and the times to discharge were 11.8, 11.2 and 10.6 min, respectively. The satisfaction ratings of anesthetists in the ciprofol 0.5 mg/kg group (9.5 ± 0.8) were higher than in the ciprofol 0.4 mg/kg (9.2 ± 1.0) and propofol 2.0 mg/kg (9.2 ± 0.9) groups. The incidence of sedation and anesthesia-related AEs was highest in the propofol 2.0 mg/kg group (25.8%), followed by the ciprofol 0.5 mg/kg group (21.9%), and was least in the ciprofol 0.4 mg/kg group (16.1%) (P = 0.750).

Conclusions: Ciprofol was safe and well tolerated at doses ranging from 0.1 mg/kg to 0.5 mg/kg. Ciprofol 0.4-0.5 mg/kg induced equivalent sedation/anesthesia and had a similar safety profile to propofol 2.0 mg/kg during colonoscopy without producing serious AEs.
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http://dx.doi.org/10.1016/j.ejps.2021.105904DOI Listing
June 2021

Enzymatically synthesised MnO nanoparticles for efficient near-infrared photothermal therapy and dual-responsive magnetic resonance imaging.

Nanoscale 2021 Jun 11. Epub 2021 Jun 11.

School of Chemistry and Chemical Engineering, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, Huazhong University of Science and Technology, Wuhan 430074, China.

Manganese dioxide (MnO2) nanoparticles (NPs) are highly attractive for biomedical applications due to their biocompatibility, stimuli-responsive magnetic resonance imaging (MRI) properties and capability to modulate the hypoxic tumour microenvironment (TME). However, conventional MnO2 NPs do not possess photothermal therapy (PTT) functions except for hybrids with other photothermal materials. Herein, we first reveal the extraordinary photothermal conversion efficiency (44%) of enzymatically synthesised MnO2 NPs (Bio-MnO2 NPs), which are distinct from chemically synthesised MnO2 NPs. In addition, the Bio-MnO2 NPs revealed high thermal recycling stability and solubility as well as dual pH- and reduction-responsive MRI enhancement for tumour theragnosis. These NPs were prepared through a facile MnxEFG enzyme-mediated biomineralization process. The MnxEFG complex from Bacillus sp. PL-12 is the only manganese mineralization enzyme that could be heterologously overexpressed in its active form to achieve Bio-MnO2 NPs without a bacterial host. The hexagonal layer symmetry of the Bio-MnO2 NPs is the key feature facilitating the high photothermal conversion efficiency and TME-responsive T1-weighted MRI. Evaluations both in vitro at the cellular level and in vivo in a systematic tumour-bearing mouse xenograft model demonstrated the high photothermal ablation efficacy of the Bio-MnO2 NPs, which achieved complete tumour eradication with high therapeutic biosafety without obvious reoccurrence. Moreover, stimuli-responsive MR enhancement potentially allows imaging-guided precision PTT. With their excellent biocompatibility, mild synthesis conditions and relatively simple composition, Bio-MnO2 NPs hold great translational promise.
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http://dx.doi.org/10.1039/d1nr02400kDOI Listing
June 2021

A New Modified Hysteroscopic-Laparoscopic Surgery for Cesarean Scar Pregnancy of Stable Type III.

Int J Gen Med 2021 3;14:2289-2295. Epub 2021 Jun 3.

Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Hubei, People's Republic of China.

Objective: To introduce a modified hysteroscopic-laparoscopic operation for cesarean scar pregnancy (CSP) of stable type III.

Patients And Methods: We retrospectively studied the case notes of 31 patients with stable type III cesarean scar pregnancy who underwent hysteroscopic-laparoscopic surgery in our hospital. Thirteen patients received the modified hysteroscopic-laparoscopic surgery (modified surgery group), and eighteen patients received traditional hysteroscopic-laparoscopic surgery (traditional surgery group).

Results: There was no significant difference in patients' age, gestational age, number of previous cesarean sections, the serum human chorionic gonadotropin (hCG) level before surgery, gestational sac diameter, myometrium thickness between the two groups. In the modified hysteroscopic-laparoscopic surgery, the mean surgical time was 50.45±24.45 mins, the mean length of stay in hospital was 4.50±0.50 days, which was significantly shorter than the traditional surgery group (84.75±33.28 mins and 5.50±0.75 days, respectively). And the intraoperative hemorrhage in the modified group was also less than that in the traditional group (40.50±12.25 mL vs 75.33±25.45mL). Whereas the time for hCG normalization, postoperative vaginal bleeding and menstrual recovery had no significant difference between the modified surgery group and the traditional surgery group. There was no recurrence of CSP in both groups.

Conclusion: The modified hysteroscopic-laparoscopic surgery had shorter operation time, less blood loss, and sooner recovery time after surgery compared to traditional hysteroscopic-laparoscopic surgery, which could be more beneficial to our patients and should be applied in clinics generally.
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http://dx.doi.org/10.2147/IJGM.S308768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184249PMC
June 2021

Trans-esophageal echocardiography guided closure of ventricular septal defect with 2 occluders from different incisions simultaneously: A case report.

Medicine (Baltimore) 2021 May;100(19):e23854

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu.

Introduction: Ventricular septal defect (VSD) accounts for up to 40% of all congenital cardiac malformations. Transthoracic closure of VSDs has been well described in literature. In the current report, we described a procedure to successfully close a VSD with 2 occluders from different incisions simultaneously under the guidance of trans-esophageal echocardiography (TEE), to save the patient from undergoing another surgery.

Patient Concerns: A 52-year-old man was referred to our clinic for repeating palpitations for 6 months without chest pain and polypnea after activity.

Diagnosis: The diagnosis of VSD was established due to the findings of a juxtatricuspid VSD with a left-to-right shunt at ventricular level and mild mitral regurgitation by TTE.

Interventions: A transcatheter VSD closure was firstly performed but failed to repair the VSD. After the failure of transcatheter VSD closure, the patient received transthoracic closure of VSD operated by a cardiac surgeon. The VSD was closed with 2 occluders from different incisions (median thoracic skin incision and subxiphoid incision) simultaneously under the TEE guidance.

Outcomes: The patient was extubated in intensive care unit and was discharged 4 days after the operation. During the follow up, there were no significant clinical nor laboratory side-effects of the procedure found as compared to the patient's condition before the procedure.

Conclusion: VSD can be closed with 2 occluders from different incisions simultaneously under the TEE guidance to save the patient from undergoing repeated surgeries. Meanwhile, TEE plays a significant role in cardiac surgery.
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http://dx.doi.org/10.1097/MD.0000000000023854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133107PMC
May 2021

Efficient Synthesis and Bioevaluation of Novel Dual Tubulin/Histone Deacetylase 3 Inhibitors as Potential Anticancer Agents.

J Med Chem 2021 Jun 7. Epub 2021 Jun 7.

School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.

Novel dual HDAC3/tubulin inhibitors were designed and efficiently synthesized by combining the pharmacophores of SMART (tubulin inhibitor) and MS-275 (HDAC inhibitor), among which compound was found to be the most potent and balanced HDAC3/tubulin dual inhibitor with high HDAC3 activity (IC = 30 nM) and selectivity (SI > 1000) as well as excellent antiproliferative potency against various cancer cell lines, including an HDAC-resistant gastric cancer cell line (YCC3/7) with IC values in the range of 30-144 nM. Compound inhibited B16-F10 cancer cell migration and colony formation. In addition, demonstrated significant antitumor efficacy in a B16-F10 melanoma tumor model with a better TGI (70.00%, 10 mg/kg) than that of the combination of MS-275 and SMART. Finally, presented a safe cardiotoxicity profile and did not cause nephro-/hepatotoxicity. Collectively, this work shows that compound represents a novel tubulin/HDAC3 dual-targeting agent deserving further investigation as a potential anticancer agent.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00413DOI Listing
June 2021

Toxicity of Lour. and its effects on lipopolysaccharide-induced inflammation and oxidative stress.

Exp Ther Med 2021 Aug 27;22(2):807. Epub 2021 May 27.

Department of Clinical Pharmacology, Lishui People's Hospital, Lishui, Zhejiang 323000, P.R. China.

Lour. (MDL) is component used in traditional Chinese medicine that is widely distributed throughout southern China. MDL has been long utilized in clinical treatment for various conditions, such as inflammation. However, the toxicity and underlying anti-inflammatory mechanism of MDL remain to be elucidated. In the present study, Sprague-Dawley rats received intragastric administration of MDL for 2 months, and the toxicity of MDL was investigated. The rats were treated with lipopolysaccharide (LPS) for 8 h to determine the potential anti-inflammatory mechanism of MDL. The results demonstrated that MDL alone did not affect the expression levels of factors associated with inflammation (IL-1β, IL-6 and TNF-α) and oxidative stress [malondialdehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO)] in the rat serum and exerted no effects on rat liver and kidneys. By contrast, MDL attenuated LPS-induced inflammation and oxidative stress by regulating specific cytokines, such as IL-1β, IL-6, TNF-α, MDA, SOD and NO in the rat serum and alleviated LPS-induced liver and kidney damage. Additionally, compared with the LPS group, MDL inhibited CD4 T cell differentiation into Th1 and Th17 cells and enhanced CD4 T cell differentiation into Th2 and Treg cells. MDL also suppressed reactive oxygen species (ROS) production and mitochondrial apoptosis by modulating mitochondrial apoptosis-related proteins in spleen CD4 T cells. In conclusion, the results of the present study demonstrated the non-toxic nature of MDL and revealed that it alleviated LPS-induced inflammation and oxidative stress by regulating differentiation and ROS production in CD4 T cells.
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http://dx.doi.org/10.3892/etm.2021.10239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170668PMC
August 2021

Multifunctional peptide-assembled micelles for simultaneously reducing amyloid-β and reactive oxygen species.

Chem Sci 2021 Apr 13;12(18):6449-6457. Epub 2021 Apr 13.

Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, East China Normal University Shanghai 200062 China

The excessive production and deposition of amyloid-β (Aβ) is one of the most important etiologies of Alzheimer's disease (AD). The interaction between Aβ and metal ions produces aberrant reactive oxygen species (ROS), which induce oxidative stress and accelerate the progression of AD. To reduce Aβ plaques and ROS to maintain their homeostasis is an emerging and ingenious strategy for effective treatment of AD. Herein, we report the rational design of multifunctional micelles (MPGLT) based on a polymer-grafted peptide to simultaneously clear Aβ and ROS for AD therapy. The MPGLT integrating three functional peptides as a ROS scavenger (tk-GSH), β-sheet breaker (LP) and an autophagy activator (TK) respectively, could capture and degrade Aβ. Meanwhile, the tk-GSH on the surface of MPGLT effectively scavenges the intracellular ROS. Consequently, MPGLT reduced the cytotoxicity of Aβ and ROS. animal studies using an AD mouse model further showed that MPGLT could transport across the blood-brain barrier for decreasing the Aβ plaque and eliminating ROS . This peptide micelle-based synergistic strategy may provide novel insight for AD therapy.
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http://dx.doi.org/10.1039/d1sc00153aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115327PMC
April 2021

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

J Extracell Vesicles 2021 May 24;10(7):e12109. Epub 2021 May 24.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases Center for Tissue Engineering School of Stomatology The Fourth Military Medical University Xi'an Shaanxi China.

Apoptosis is a naturally occurring process generating plenty of apoptotic vesicles (apoVs), but the feature, fate and function of apoVs remain largely unknown. Notably, as an appealing source for cell therapy, mesenchymal stem cells (MSCs) undergo necessary apoptosis and release apoVs during therapeutic application. In this study, we characterized and used MSC-derived apoVs to treat type 2 diabetes (T2D) mice, and we found that apoVs were efferocytosed by macrophages and functionally modulated liver macrophage homeostasis to counteract T2D. We showed that apoVs can induce macrophage reprogramming at the transcription level in an efferocytosis-dependent manner, leading to inhibition of macrophage accumulation and transformation of macrophages towards an anti-inflammation phenotype in T2D liver. At the molecular level, we discovered that calreticulin (CRT) was exposed on the surface of apoVs to act as a critical 'eat-me' signal mediating apoV efferocytosis and macrophage regulatory effects. Importantly, we demonstrated that CRT-mediated efferocytosis of MSC-derived apoVs contributes to T2D therapy with alleviation of T2D phenotypes including glucose intolerance and insulin resistance. These findings uncover that functional efferocytosis of apoVs restores liver macrophage homeostasis and ameliorates T2D.
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http://dx.doi.org/10.1002/jev2.12109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144839PMC
May 2021

The global incidence and mortality of contrast-associated acute kidney injury following coronary angiography: a meta-analysis of 1.2 million patients.

J Nephrol 2021 Jun 2. Epub 2021 Jun 2.

Department of Cardiology, Guangdong Provincial Key Laboratory of Coronary Heart Disease Prevention, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China.

Background: Contrast-associated acute kidney injury (CA-AKI) is a common complication after coronary angiography (CAG), which brings a poor prognosis. But up to now, there were fewer studies to discuss the incidence of CA-AKI comprehensively. We comprehensively explore the incidence of CA-AKI after coronary angiography.

Methods: We searched Medline, Embase, and Cochrane Database of Systematic Reviews (to 30th June 2019). We evaluated the world's incidence of the CA-AKI, and associated mortality, and to described geographic variations according to countries, regions, and economies. CA-AKI was defined as an increase in serum creatinine ≥ 0.5 mg/dl or ≥ 25% within 72 h. Random effects model meta-analyses and meta-regressions was performed to derive the sources of heterogeneity.

Results: A total of 134 articles (1,211,106 participants) were included in our meta-analysis. Most studies originated from China, Japan, Turkey and United States, from upper middle income and high income countries. The pooled incidence of CA-AKI after coronary angiography was 12.8% (95% CI 11.7-13.9%), and the CA-AKI associated mortality was 20.2% (95% CI 10.7-29.7%). The incidence of CA-AKI and the CA-AKI associated mortality were not declined over time (Incidence rate change: 0.23% 95% CI - 0.050 to 0.510 p = 0.617; Mortality rate change: - 1.05% 95% CI - 3.070 to 0.970 p = 0.308, respectively).

Conclusion: CA-AKI was a universal complication in many regions, and the burden of CA-AKI remains severe. In clinical practice, physicians should pay more attention to the occurrence and active prevention and treatment of CA-AKI.
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http://dx.doi.org/10.1007/s40620-021-01021-1DOI Listing
June 2021

Subchronic Oral Toxicity Study of Genetically Modified Rice Rich in β-Carotene in Wistar Rats.

Int J Environ Res Public Health 2021 May 21;18(11). Epub 2021 May 21.

Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China.

(1) Background: a hybrid black rice rich in β-carotene carrying the and genes (HJM) was evaluated in Wistar rats by a 90-day feeding study, aiming to assess its dietary safety. (2) Methods: the HJM rice and its parental line HS were included in rats' diets at levels of 73.5% and 75.5%, respectively. The AIN-93 diet was administered as a nutritional control. No adverse effects on animal behavior or weight gain were observed during the study. Blood samples were collected and analyzed, and standard hematological and biochemical parameters were compared. (3) Results: Some parameters were found to be significantly different, though they remained within the normal range for rats of this breed and age. In addition, upon sacrifice, various organs were weighed, and macroscopic and histopathological examinations were performed, with only minor changes to report. (4) Conclusions: HJM rice exhibited no adverse or toxic effects in Wistar rats in this 90-day study.
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http://dx.doi.org/10.3390/ijerph18115526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196761PMC
May 2021

Intestinal submucous fibrovascular hamartoma: A case report.

Radiol Case Rep 2021 Jul 23;16(7):1857-1861. Epub 2021 May 23.

Number 155, Nanjing North Street, Heping District, Shenyang City, Liaoning Province, China.

Intestinal submucosal fibrovascular hamartoma is considered as a rare intestinal lesion. We present the case of a 63-year-old female with abdominal symptoms, bleeding, and increased serum tumor markers. The abdominal ultrasound revealed that the left abdominal intestinal wall and mesentery were thickened with enlarged multiple lymph nodes, suggesting intestinal obstruction. Other imaging findings confirmed the ultrasound findings. Histopathology of the removed lesion provided the diagnosis of intestinal submucosal fibrovascular hamartoma with hemorrhage, inflammation, and amyloidosis. Intestinal submucosal fibrovascular hamartoma is a hemorrhagic lesion with macroscopic tumor due to the abnormal mixing of the organ's normal components, which still remains a challenge for clinicians and pathologists. We consider routine abdominal ultrasonography and contrast-enhanced ultrasonography (SICUS) to be safe and effective in the diagnosis of intestinal neoplastic lesions.
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http://dx.doi.org/10.1016/j.radcr.2021.04.048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144528PMC
July 2021

A Bioinformatics Investigation into the Pharmacological Mechanisms of Sodium-Glucose Co-transporter 2 Inhibitors in Diabetes Mellitus and Heart Failure Based on Network Pharmacology.

Cardiovasc Drugs Ther 2021 May 24. Epub 2021 May 24.

Guangdong Provincial People's Hospital, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.

Purpose: Diabetes mellitus (DM) is a major risk factor for the development of heart failure (HF). Sodium-glucose co-transporter 2 (SGLT2) inhibitors have demonstrated consistent benefits in the reduction of hospitalization for HF in patients with DM. However, the pharmacological mechanism is not clear. To investigate the mechanisms of SGLT2 inhibitors in DM with HF, we performed target prediction and network analysis by a network pharmacology method.

Methods: We selected targets of SGLT2 inhibitors and DM status with HF from databases and studies. The "Drug-Target" and "Drug-Target-Disease" networks were constructed using Cytoscape. Then the protein-protein interaction (PPI) was analyzed using the STRING database. Gene Ontology (GO) biological functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were performed to investigate using the Bioconductor tool for analysis.

Results: There were 125 effective targets between SGLT2 inhibitors and DM status with HF. Through further screening, 33 core targets were obtained, including SRC, MAPK1, NARS, MAPK3 and EGFR. It was predicted that the Rap1 signaling pathway, MAPK signaling pathway, EGFR tyrosine kinase inhibitor resistance, AGE-RAGE signaling pathway in diabetic complications and other signaling pathways were involved in the treatment of DM with HF by SGLT2 inhibitors.

Conclusion: Our study elucidated the possible mechanisms of SGLT2 inhibitors from a systemic and holistic perspective based on pharmacological networks. The key targets and pathways will provide new insights for further research on the pharmacological mechanism of SGLT2 inhibitors in the treatment of DM with HF.
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http://dx.doi.org/10.1007/s10557-021-07186-yDOI Listing
May 2021

Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes.

Authors:
Jin Liu Hongzhou Gu

STAR Protoc 2021 Jun 10;2(2):100531. Epub 2021 May 10.

Fudan University Shanghai Cancer Center and the Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical Sciences, Shanghai Stomatological Hospital, Fudan University, Shanghai 200433, China.

Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable sequence up to a few kilobases. Our protocol provides a convenient yet economical way to store the sequence information of target ssDNA on phages for selective mass production on demand. For complete details on the use and execution of this protocol, please refer to Jia et al. (2021).
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http://dx.doi.org/10.1016/j.xpro.2021.100531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134756PMC
June 2021

Prognostic Value of Tumor-Associated Macrophages in Clear Cell Renal Cell Carcinoma: A Systematic Review and Meta-Analysis.

Front Oncol 2021 26;11:657318. Epub 2021 Apr 26.

Department of Urology, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Tumor-associated macrophages (TAMs) are the major immune cells in tumor microenvironment. The prognostic significance of TAMs has been confirmed in various tumors. However, whether TAMs can be prognostic factors in clear cell renal cell carcinoma (ccRCC) is unclear. In this study, we aimed to clarify the prognostic value of TAMs in ccRCC.

Methods: We searched PubMed, Embase, and the Web of Science for relevant published studies before December 19, 2020. Evidence from enrolled studies were pooled and analyzed by a meta-analysis. Hazard ratios (HRs) and odd ratios (ORs) with 95% confidence intervals (CIs) were computed to evaluate the pooled results.

Results: Both of high CD68+ TAMs and M2-TAMs were risk factors for poor prognosis in ccRCC patients. The pooled HRs indicated that elevated CD68+ TAMs correlated with poor OS and PFS (HR: 3.97, 95% CI 1.39-11.39; HR: 5.73, 95% CI 2.36-13.90, respectively). For M2-TAMs, the pooled results showed ccRCC patients with high M2-TAMs suffered a worse OS and shorter PFS, with HR 1.32 (95% CI 1.16-1.50) and 1.40 (95% CI 1.14-1.72), respectively. Also, high density of TAMs was associated with advanced clinicopathological features in ccRCC.

Conclusions: TAMs could be potential biomarkers for prognosis and novel targets for immunotherapy in ccRCC. Further researches are warranted to validate our results.
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http://dx.doi.org/10.3389/fonc.2021.657318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136289PMC
April 2021

Functional connectivity evidence for state-independent executive function deficits in patients with major depressive disorder.

J Affect Disord 2021 Aug 21;291:76-82. Epub 2021 May 21.

Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China; Mental Health Institute of Central South University, China National Clinical Research Center on Mental Disorders (Xiangya), China National Technology Institute on Mental Disorders, Hunan Technology Institute of Psychiatry, Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, Hunan 410011, China. Electronic address:

Background: Persistent neurocognitive deficits are often associated with poor outcomes of major depressive disorder (MDD). Executive dysfunction is the most common cognitive deficit in MDD. However, it remains unclear which subcomponent of executive dysfunction is state-independent with distinct neural substrates.

Methods: A comprehensive neurocognitive test battery was used to assess four subcomponents of executive function (working memory, inhibition, shifting, and verbal fluency) in 95 MDD patients and 111 matched healthy controls (HCs). After 6 months of paroxetine treatment, 56 patients achieved clinical remission (rMDD) and completed the second-time neurocognitive test. Network-based statistics analysis was utilized to explore the changes in functional connectivity (FC).

Results: Compared with the HCs, all the four subcomponents of MDD patients were significantly impaired. After treatment, there was a significant improvement in working memory, inhibition, and verbal fluency in the rMDD group. And shifting and verbal fluency of the rMDD group remained impaired compared with the HCs. Fifteen functional connections were interrupted in the MDD group, and 11 connections remained in a disrupted state after treatment. Importantly, verbal fluency was negatively correlated with the disrupted FC between the right dorsal prefrontal cortex and the left inferior parietal lobule in patients with MDD and remitted MDD.

Limitations: The correlation analysis of the association between cognitive impairment and connectivity alterations precluded us from making causal inferences.

Conclusions: Verbal fluency is the potential state-independent cognitive deficit with distinct neural basis in patients with MDD.
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http://dx.doi.org/10.1016/j.jad.2021.04.080DOI Listing
August 2021

Free Fatty Acid is a Promising Biomarker in Triage Screening for Patients with Colorectal Cancer: A Case-Control Study.

Cancer Manag Res 2021 10;13:3749-3759. Epub 2021 May 10.

Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, 350001, People's Republic of China.

Purpose: The aim of our study was to identify the diagnostic ability of free fatty acids (FFAs) in younger colorectal cancer (CRC) patients by comparing carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9).

Methods: Patients screened for CRC at Fujian Medical University Union Hospital from January 2011 to December 2014 were recruited. Patients pathologically diagnosed with CRC or colorectal adenoma (CA) and healthy control participants were included. The enzyme endpoint method was applied to measure FFA levels. Receiver operating characteristic (ROC) curve analysis was performed to further evaluate the diagnostic ability of FFAs.

Results: FFA levels in late-stage patients (tumour-node-metastasis (TNM) stages III-IV) were higher than those in early-stage patients (TNM stages I-II) (P=0.02). The FFA levels in CRC patients were higher than those in controls of all ages, those younger than 50 years, males and females (P<0.001), and this difference was larger for patients younger than 50 years and females than for the all ages group. There was no significant difference in the FFA level between CA patients and healthy participants (P=0.53). The area under the curve (AUC) values of FFA, CEA, CA19-9, FFA+CEA, FFA+CA19-9 and FFA+CEA+CA19-9 distinguished CRC patients from controls at all ages, with values of 0.604, 0.731, 0.640, 0.754, 0.678 and 0.758, respectively; however, in the younger CRC patients (age≤50), the AUC values were 0.701, 0.735, 0.669, 0.798, 0.749, and 0.801. The AUC in female patients younger than 50 years was larger than that in males (0.769 vs 0.660), and this value was greater than the value for CEA in males (0.739) and females (0.729).

Conclusion: The FFA level not only can complement the predictive ability of the CEA and CA19-9 levels but also has a superior predictive ability in female and younger patients with CRC. FFA levels may have a potential role in triage screening of early CRC.
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http://dx.doi.org/10.2147/CMAR.S307753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123087PMC
May 2021

Analysis of radiotherapy impact on survival in resected stage I/II pancreatic cancer patients: a population-based study.

BMC Cancer 2021 May 17;21(1):560. Epub 2021 May 17.

Department of Oncology & Hematology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, No.23 Back street in the Museum of Art Rd, Dongcheng District, Beijing, China.

Background: The application of radiotherapy (RT) in pancreatic cancer remains controversial.

Aim: The aim of the study was to evaluate the efficacy of radiotherapy (neoadjuvant and adjuvant radiotherapy) for resectable I/II pancreatic cancer.

Methods: Fourteen thousand nine hundred seventy-seven patients with pancreatic cancer were identified from SEER database from 2004 to 2015. Multivariate analyses were performed to determine factors including RT on overall survival. Overall survival and overall mortality among the different groups were evaluated using the Kaplan-Meier method and Gray's test.

Results: Patients were divided into groups according to whether they received radiotherapy or not. The median survival time of all 14,977 patients without RT was 20 months, neoadjuvant RT was 24 months and adjuvant RT was 23 months (p < 0.0001). Median survival time of 2089 stage I patients without RT was 56 months, significantly longer than those with RT regardless of neoadjuvant or adjuvant RT (no RT: 56 months vs adjuvant RT: 37 months vs neoadjuvant RT: 27 months, P = 0.0039). Median survival time of 12,888 stage II patients with neoadjuvant RT was 24 months, adjuvant RT 22 months, significantly prolonged than those without radiotherapy (neoadjuvant RT: 24 months vs adjuvant RT: 22 months vs no RT: 17 months, P<0.0001). Neoadjuvant RT (HR = 1.434, P = 0.023, 95% CI: 1.051-1.957) was independent risk factors for prognosis of stage I patients, and adjuvant RT (HR = 0.904, P < 0.001, 95% CI: 0.861-0.950) predicted better outcomes for prognosis of stage II patients by multivariate analysis. The risk of cancer-related death caused by neoadjuvant RT in stage I and no-RT in stage II patients were significantly higher.

Conclusions: The study identified a significant survival advantage for the use of adjuvant RT over surgery alone or neoadjuvant RT in treating stage II pancreatic cancer. RT was not associated with survival benifit in stage I patients.
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http://dx.doi.org/10.1186/s12885-021-08288-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130297PMC
May 2021

Low-dose CT imaging via cascaded ResUnet with spectrum loss.

Methods 2021 May 14. Epub 2021 May 14.

Key Laboratory of Computer Network and Information Integration (Southeast University) Ministry of Education Nanjing, China; School of Cyber Science and Engineering, Southeast University, Nanjing, China; School of Computer Science and Engineering, Southeast University, Nanjing, China.

The suppression of artifact noise in computed tomography (CT) with a low-dose scan protocol is challenging. Conventional statistical iterative algorithms can improve reconstruction but cannot substantially eliminate large streaks and strong noise elements. In this paper, we present a 3D cascaded ResUnet neural network (Ca-ResUnet) strategy with modified noise power spectrum loss for reducing artifact noise in low-dose CT imaging. The imaging workflow consists of four components. The first component is filtered backprojection (FBP) reconstruction via a domain transformation module for suppressing artifact noise. The second is a ResUnet neural network that operates on the CT image. The third is an image compensation module that compensates for the loss of tiny structures, and the last is a second ResUnet neural network with modified spectrum loss for fine-tuning the reconstructed image. Verification results based on American Association of Physicists in Medicine (AAPM) and United Image Healthcare (UIH) datasets confirm that the proposed strategy significantly reduces serious artifact noise while retaining desired structures.
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http://dx.doi.org/10.1016/j.ymeth.2021.05.005DOI Listing
May 2021

The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.

Biotechnol Biofuels 2021 May 15;14(1):119. Epub 2021 May 15.

Laboratory for Algae Biotechnology and Innovation, College of Engineering, Peking University, Beijing, 100871, China.

The algal lipids-based biodiesel, albeit having advantages over plant oils, still remains high in the production cost. Co-production of value-added products with lipids has the potential to add benefits and is thus believed to be a promising strategy to improve the production economics of algal biodiesel. Chromochloris zofingiensis, a unicellular green alga, has been considered as a promising feedstock for biodiesel production because of its robust growth and ability of accumulating high levels of triacylglycerol under multiple trophic conditions. This alga is also able to synthesize high-value keto-carotenoids and has been cited as a candidate producer of astaxanthin, the strongest antioxidant found in nature. The concurrent accumulation of triacylglycerol and astaxanthin enables C. zofingiensis an ideal cell factory for integrated production of the two compounds and has potential to improve algae-based production economics. Furthermore, with the advent of chromosome-level whole genome sequence and genetic tools, C. zofingiensis becomes an emerging model for studying lipid metabolism and carotenogenesis. In this review, we summarize recent progress on the production of triacylglycerol and astaxanthin by C. zofingiensis. We also update our understanding in the distinctive molecular mechanisms underlying lipid metabolism and carotenogenesis, with an emphasis on triacylglycerol and astaxanthin biosynthesis and crosstalk between the two pathways. Furthermore, strategies for trait improvements are discussed regarding triacylglycerol and astaxanthin synthesis in C. zofingiensis.
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http://dx.doi.org/10.1186/s13068-021-01969-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126118PMC
May 2021

Factors Governing Selectivity of Dopamine Receptor Binding Compounds for D2R and D3R Subtypes.

J Chem Inf Model 2021 May 14. Epub 2021 May 14.

Department of Pharmaceutical Sciences, University of North Texas System College of Pharmacy, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, Texas 76107, United States.

Targeting the D3 dopamine receptor (D3R) is a promising pharmacotherapeutic strategy for the treatment of many disorders. The structure of the D3R is similar to the D2 dopamine receptor (D2R), especially in the transmembrane spanning regions that form the orthosteric binding site, making it difficult to identify D3R selective pharmacotherapeutic agents. Here, we examine the molecular basis for the high affinity D3R binding and D3R vs D2R binding selectivity of substituted phenylpiperazine thiopheneamides. We show that removing the thiophenearylamide portion of the ligand consistently decreases the affinity of these ligands at D3R, while not affecting their affinity at the D2R. Our long (>10 μs) molecular dynamics simulations demonstrated that both dopamine receptor subtypes adopt two major conformations that we refer to as closed or open conformations, with D3R sampling the open conformation more frequently than D2R. The binding of ligands with conjoined orthosteric-allosteric binding moieties causes the closed conformation to populate more often in the trajectories. Also, significant differences were observed in the extracellular loops (ECL) of these two receptor subtypes leading to the identification of several residues that contribute differently to the ligand binding for the two receptors that could potentially contribute to ligand binding selectivity. Our observations also suggest that the displacement of ordered water in the binding pocket of D3R contributes to the affinity of the compounds containing an allosteric binding motif. These studies provide a better understanding of how a bitopic mode of engagement can determine ligands that bind selectively to D2 and D3 dopamine receptor subtypes.
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http://dx.doi.org/10.1021/acs.jcim.1c00036DOI Listing
May 2021

Rapid ATF4 Depletion Resets Synaptic Responsiveness after cLTP.

eNeuro 2021 May-Jun;8(3). Epub 2021 Jun 3.

Department of Pathology and Cell Biology, Columbia University Medical Center, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York 10032

Activating transcription factor 4 [ATF4 (also called CREB2)], in addition to its well studied role in stress responses, is proposed to play important physiologic functions in regulating learning and memory. However, the nature of these functions has not been well defined and is subject to apparently disparate views. Here, we provide evidence that ATF4 is a regulator of excitability during synaptic plasticity. We evaluated the role of ATF4 in mature hippocampal cultures subjected to a brief chemically induced LTP (cLTP) protocol that results in changes in mEPSC properties and synaptic AMPA receptor density 1 h later, with return to baseline by 24 h. We find that ATF4 protein, but not its mRNA, is rapidly depleted by ∼50% in response to cLTP induction via NMDA receptor activation. Depletion is detectable in dendrites within 15 min and in cell bodies by 1 h, and returns to baseline by 8 h. Such changes correlate with a parallel depletion of phospho-eIF2a, suggesting that ATF4 loss is driven by decreased translation. To probe the physiologic role of cLTP-induced ATF4 depletion, we constitutively overexpressed the protein. Reversing ATF4 depletion by overexpression blocked the recovery of synaptic activity and AMPA receptor density to baseline values that would otherwise occur 24 h after cLTP induction. This reversal was not reproduced by a transcriptionally inactive ATF4 mutant. These findings support the role of ATF4 as a required element in resetting baseline synaptic responsiveness after cLTP.
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http://dx.doi.org/10.1523/ENEURO.0239-20.2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177969PMC
June 2021

The influence on oxidative stress markers, inflammatory factors and intestinal injury-related molecules in Wahui pigeon induced by lipopolysaccharide.

PLoS One 2021 12;16(5):e0251462. Epub 2021 May 12.

College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China.

Introduction: The intestinal structure is the foundation for various activities and functions in poultry. An important question concerns the changes in the intestinal status under endotoxin stimulation. This study aimed to investigate the mechanism of intestinal injury induced by lipopolysaccharide (LPS) in Wahui pigeons.

Methods: Thirty-six 28-day-old healthy Wahui pigeons were randomly divided into two groups. The experimental group was injected with LPS (100 μg/kg) once per day for five days, and the control group was treated with the same amount of sterile saline. Blood and the ileum were collected from pigeons on the first, third, and fifth days of the experiment and used for oxidative stress assessment, inflammatory factor detection, histopathological examination, and positive cell localization. In addition, intestinal injury indices and mRNA expression levels (tight junction proteins, inflammatory cytokines, and factors related to autophagy and apoptosis) were evaluated.

Results: Villi in the ileum were shorter in the LPS group than in the control group, and D-lactic acid levels in the serum were significantly increased. Glutathione and catalase levels significantly decreased, but the malondialdehyde content in the serum increased. TNF-α and IL-10 were detected at higher levels in the serum, with stronger positive signals and higher mRNA expression levels, in the LPS group than in the control group. In addition, the levels of TLR4, MyD88, NF-κB, and HMGB1 in the inflammatory signaling pathway were also upregulated. Finally, the mRNA expression of Claudin3, Occludin, and ZO-1 was significantly decreased; however, that of Beclin1 and Atg5 was increased in the LPS group.

Conclusion: Ileal pathological changes and oxidative stress were caused by LPS challenge; it is proposed that this triggering regulates the inflammatory response, causing excessive autophagy and apoptosis, promoting intestinal permeability, and leading to intestinal injury in Wahui pigeons.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251462PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115843PMC
May 2021

Cornuside alleviates experimental autoimmune encephalomyelitis by inhibiting Th17 cell infiltration into the central nervous system.

J Zhejiang Univ Sci B 2021 May;22(5):421-430

College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.

The present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. On the second day after EAE rats began to show neurological deficit symptoms, the four groups were treated with normal saline, normal saline, cornuside (150 mg/kg), and prednisolone (5 mg/kg), respectively. The treatment was discontinued after two weeks, and the spinal cord was obtained for hematoxylin and eosin (H&E) and luxol fast blue staining, as well as retinoic acid receptor-related orphan receptor γ (RORγ) and forkhead box protein P3 (Foxp3) immunohistochemical staining. Blood was collected for Th17 and Treg cell flow cytometry testing, and the serum levels of interleukin (IL)-17A, IL-10, transforming growth factor-β (TGF-β), IL-6, IL-23, and IL-2 were measured via enzyme-linked immunosorbent assay (ELISA). Compared with rats in the EAE group, rats in the EAE+cornuside and EAE+prednisolone groups began to recover from neurological deficits earlier, and had a greater degree of improvement of symptoms. Focal inflammation, demyelination, and RORγ-positive cell infiltration were reduced by cornuside or prednisolone treatment, whereas the Foxp3-positive cell numbers were not significantly different. Meanwhile, the number of Th17 cells and the IL-17A, IL-6, and IL-23 levels were lower in the blood after cornuside or prednisolone treatment, whereas the number of Treg cells or the levels of IL-10, TGF-β, and IL-2 were not markedly different. Cornuside can alleviate symptoms of EAE neurological deficits through its anti-inflammatory and immunosuppressive effects, and Th17 cells may be one of its therapeutic targets.
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http://dx.doi.org/10.1631/jzus.B2000771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110462PMC
May 2021

[Effects of Vitrification with Self-made Carriers and Slow Programmed Freezing on Ovarian Tissue of Sheep].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Apr;43(2):193-198

Infertility Center,Qilu Hospital of Shandong University,Jinan 250012,China.

Objective To investigate the effects of self-made carriers on the cryopreservation of ovarian tissue of sheep. Methods Thirty-two ovaries were randomly assigned to fresh group,programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group.The morphology,proliferation,apoptosis,and estrogen level of the ovarian tissue in each group were observed. Results After cryopreservation,the morphology normal rate of the primordial follicles in programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group were 74.2%,72.8%,and 72.3%,respectively,lower than that(83.7%)in the fresh group(χ=13.079,P=0.004).The percentage of normal primary follicles in programmed freezing group was lower than that in the fresh group(χ=12.486,P=0.000).The percentage of normal primary follicles showed no significant difference between vitrification groups and fresh group(P=1.000,P=0.972).There was no significant difference in estrogen level or the positive expression rate of PCNA among the 4 groups(F=0.363,P=0.780;χ=0.359,P=0.949).The number of apoptotic cells in cryopreservation groups was significantly higher than that in the fresh group(F=37.584,P=0.000),and it was significantly higher in the programmed freezing group than in the two vitrification groups(F=18.992,P=0.000). Conclusion Compared with slow programmed freezing,the vitrification with self-made carriers could well preserve the activity of cells in large sheep ovarian tissue blocks.
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http://dx.doi.org/10.3881/j.issn.1000-503X.12651DOI Listing
April 2021

A Psychometric Evaluation of the Quality of Life for Children with Autism Spectrum Disorder Scale.

J Autism Dev Disord 2021 May 7. Epub 2021 May 7.

The Faison Center, 1701 Byrd Avenue, Richmond, VA, 23230, USA.

Our purpose in this study was to validate the Quality of Life for Children with Autism Spectrum Disorder scale. We first conducted an exploratory factor analysis to examine the factor structure. Results suggested that a three-factor model (Interpersonal Relationships, Self-Determination, and Emotional Well-Being) should be retained. Next, we conducted a confirmatory factor analysis to compare a higher-order factor model and additional models. Results showed acceptable model fit for the higher-order factor model. The scale had excellent reliability (α = .90) for the overall scale and for the three subscales (Interpersonal Relationships, α = .87; Self-Determination, α = .81; and Emotional Well-Being, α = .66). The 16-item scale showed convergent and divergent validity. Implications for future research and practice are discussed.
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http://dx.doi.org/10.1007/s10803-021-05048-yDOI Listing
May 2021

Analysis based on Monte Carlo simulation: How effective is tigecycline in routine antimicrobial therapy?

Int J Clin Pharmacol Ther 2021 May 7. Epub 2021 May 7.

Objective: This paper aims to assess the efficacy of tigecycline in the treatment of several different infections from a pharmacokinetic/pharmacodynamic (PK/PD) perspective.

Materials And Methods: The minimum inhibitory concentration (MIC) test strip test was used to determine the MICs of clinical isolates of tigecycline. A 5,000-subjects simulation was performed by Crystal Ball software to calculate the probability of achieving the required PK/PD exposure.

Results: The use of standard tigecycline dosing is predicted to have a good clinical outcome for patients suffering from complicated skin and skin structure infection (cSSSI) with MICs ≤ 0.25 mg×L-1, patients suffering from complicated intra-abdominal infection (cIAI) with MICs ≤ 1 mg×L-1, and patients suffering from hospital-acquired pneumonia (HAP) with MICs ≤ 0.5 mg×L-1. Generally, Gram-positive bacteria are highly sensitive to tigecycline, while Gram-negative bacteria are less sensitive: for patients with HAP and cIAI, the tolerable outcome was achieved using the standard regimen for most Gram-negative pathogens; the desired outcomes could be obtained for the increased-dose treatment; with increasing dose (100 mg every 12 hours), the average cumulative fractions of response (CFRs) markedly increased from 38.18 to 56.21% for cSSSI patients. When tigecycline, a standard regimen, was used to treat carbapenem-resistant (CRKP) and carbapenem-resistant . (CRE) infections, the cumulative response scores were 4.96 - 66.39% and 13.14 - 95.18%, respectively, and the CFRs of the increased dose also increased correspondingly.

Conclusion: Currently, the standard dose of tigecycline is feasible in the treatment of common bacterial infections, and PK/PD indexes are needed to optimize the regimens for refractory carbapenem-resistant bacterial infections.
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http://dx.doi.org/10.5414/CP203903DOI Listing
May 2021

Synthesis of Ni/nano-AlO coatings by brush plating with magnetic fields.

R Soc Open Sci 2021 Mar 24;8(3):202089. Epub 2021 Mar 24.

Institute of Surface/Interface Science and Technology, Key Laboratory of Superlight Material and Surface Technology of Ministry of Education, Harbin Engineering University, Harbin 150001, People's Republic of China.

In the process of electrodeposition, the magnetic field would generate the magneto hydrodynamic (MHD) effect, which affects the flow and mass transfer of hydrogen evolution. Thus, the performance of electrodeposition coatings will be affected. In this work, Ni/nano-AlO coatings prepared by brush plating with magnetic fields were studied. The results show that the magnetic field indeed influences the morphology, texture and wears resistance. The morphology of Ni/AlO coating is smooth and uniform; the (200) plane of Ni/AlO coating is preferentially oriented in the same direction of a magnetic field; the wear resistance of Ni/AlO composite coatings increases due to the uniform distribution of AlO particles; and the wear mechanism of coating belongs to adhesive wear. The optimal intensity of magnetic field for Ni/AlO composite coatings to obtain a good performance is 0.1 T.
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http://dx.doi.org/10.1098/rsos.202089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074909PMC
March 2021