Publications by authors named "Jin Li"

4,417 Publications

  • Page 1 of 1

HMGA2-Snai2 axis regulates tumorigenicity and stemness of head and neck squamous cell carcinoma.

Exp Cell Res 2022 Jun 25:113271. Epub 2022 Jun 25.

Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China; Jiangsu Key Laboratory of Oral Disease, Nanjing Medical University, Nanjing, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing Medical University, Nanjing, China. Electronic address:

Cancer stem cells (CSCs) are a tumorigenic cell subpopulation, which contributes to treatment resistance, tumor recurrence, and metastasis. This study aimed to investigate the role and underlying molecular targets of high mobility group AT-hook 2 (HMGA2) in the progression and CSCs regulation of head and neck squamous cell carcinoma (HNSCC). HMGA2 mRNA and protein expression levels were examined in HNSCC specimens and cells by qRT-PCR, Western blot, and immunohistochemistry. The roles of HMGA2 were validated via loss-of-function and exogenous overexpression experiments in vitro and in vivo, and CSCs properties were assessed by tumorsphere formation assay. Chromatin immunoprecipitation (ChIP) and luciferase reporter assays provided further insight into the molecular mechanisms by which HMGA2 regulates stemness. HMGA2 was abnormally overexpressed in HNSCC, and it promoted the expression of the CSCs markers including SOX2, CD133, CD44, ALDH1A1, and Bmi1. HMGA2 was correlated with stemness, malignant progression, and reduced survival in HNSCC. Luciferase reporter assay indicated that Snai2 was a direct downstream target gene of HMGA2. Mechanistically, ChIP-qPCR assay showed that HMGA2 was recruited to three binding sites on the Snai2 promoter, directly facilitating the transcription of Snai2 in HNSCC. Snai2 overexpression reversed the inhibitory effect of HMGA2 interference on the proliferation, invasion, and metastasis of HNSCC and CSC marker expression in vitro and in vivo. HMGA2 promoted the malignant progression of HNSCC and acquired CSCs properties through direct regulation of Snai2, thereby suggesting that targeting the HMGA2-Snai2 axis might be a promising therapeutic strategy for HNSCC.
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http://dx.doi.org/10.1016/j.yexcr.2022.113271DOI Listing
June 2022

Effects of rhythmic auditory stimulation on upper-limb movements in patients with Parkinson's disease.

Parkinsonism Relat Disord 2022 Jun 23;101:27-30. Epub 2022 Jun 23.

Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Introduction: Rhythmic auditory stimulation (RAS) is an effective technique extensively used to alleviate lower-limb bradykinesia in patients with Parkinson's disease (PD). However, RAS effects on upper-limb bradykinesia have not been well studied. This study investigated immediate effects of RAS on upper-limb movements in PD patients and healthy people.

Methods: PD patients (n = 23) and age- and gender-matched healthy controls (n = 23) executed left-hand, right-hand, and both-hand movement tasks of the Purdue Pegboard Test when listening to the beats of RAS, including 100%, 110%, and 120% of the baseline tempo, which was fastest movement performance of each participant without the aid of RAS. Sequence of RAS and tasks was randomized for each participant.

Results: PD patients had slower upper-limb movements than did health controls. An interaction was found between RAS and tasks. In both patients and controls and for all task conditions, 120%RAS induced higher scores than did 110% RAS, and the latter induced higher scores than did 100%RAS. In both patients and controls and for all RAS conditions, the right-hand condition induced higher scores than did the left-hand condition, and the latter induced higher scores than did the both-hand condition.

Conclusions: RAS was effective in regulating upper-limb movements in PD patients, which may be explained by rich neural connections between auditory and motor cortical areas in humans. Clinical practitioners should consider using RAS in clinical therapy. Future neuroimaging studies are needed to explore neural mechanisms of RAS in PD patients.
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http://dx.doi.org/10.1016/j.parkreldis.2022.06.020DOI Listing
June 2022

Delivery of engineered extracellular vesicles with miR-29b editing system for muscle atrophy therapy.

J Nanobiotechnology 2022 Jun 27;20(1):304. Epub 2022 Jun 27.

Institute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.

Muscle atrophy is a frequently observed complication, characterized by the loss of muscle mass and strength, which diminishes the quality of life and survival. No effective therapy except exercise is currently available. In our previous study, repressing miR-29b has been shown to reduce muscle atrophy. In our current study, we have constructed artificially engineered extracellular vesicles for the delivery of CRISPR/Cas9 to target miR-29b (EVs-Cas9-29b). EVs-Cas9-29b has shown a favorable functional effect with respect to miR-29b repression in a specific and rapid manner by gene editing. In in vitro conditions, EVs-Cas9-29b could protect against muscle atrophy induced by dexamethasone (Dex), angiotensin II (AngII), and tumor necrosis factor-alpha (TNF-α). And EVs-Cas9-29b introduced in vivo preserved muscle function in the well-established immobilization and denervation-induced muscle atrophy mice model. Our work demonstrates an engineered extracellular vesicles delivery of the miR-29b editing system, which could be potentially used for muscle atrophy therapy.
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http://dx.doi.org/10.1186/s12951-022-01508-4DOI Listing
June 2022

Proactive functional classification of all possible missense single-nucleotide variants in .

Genome Res 2022 Jun 27. Epub 2022 Jun 27.

Fudan University;

Clinical exome sequencing has yielded extensive disease-related missense single-nucleotide variants (SNVs) with uncertain significance, leading to diagnostic uncertainty. is one of the most commonly responsible genes for autosomal dominant nonsyndromic hearing loss. According to the gnomAD cohort, approximately 1 in 100 people harbor missense variants in (missense variants with minor allele frequency > 0.1% were excluded), but most are of unknown consequence. To prospectively characterize the function of all 4,085 possible missense SNVs of human , we recorded the whole-cell currents using the patch-clamp and categorized 1,068 missense SNVs as loss-of-function, as well as 728 loss-of-function SNVs located in the transmembrane domains. Further, to mimic the heterozygous condition in Deafness nonsyndromic autosomal dominant 2 (DFNA2) patients caused by variants, we coexpressed loss-of-function variants with wild-type and found 516 variants exhibited impaired or only partial rescued heterogeneous channel function. Overall, our functional classification is highly concordant with the auditory phenotypes in mutant mice and the assessments of pathogenicity in clinical variant interpretations. Taken together, our results can provide strong functional evidence to support the pathogenicity classification of newly discovered missense variants in clinical genetic testing.
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http://dx.doi.org/10.1101/gr.276562.122DOI Listing
June 2022

SEMA6D, Negatively Regulated by miR-7, Contributes to C28/I2 chondrocyte's Catabolic and Anabolic Activities via p38 Signaling Pathway.

Oxid Med Cell Longev 2022 16;2022:9674221. Epub 2022 Jun 16.

Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou 213000, China.

MiR-7 has been recognized as an osteoarthritis (OA-)-promoting factor, but the specific downstream pathway of miR-7 still remains unknown. Further investigation of the molecular regulatory mechanism of miR-7 might help develop a novel therapeutic method for OA. In this study, we revealed that Semaphorin 6D (SEMA6D) was a direct target gene of miR-7 and presented a negative regulatory relation with SEMA6D and . SEMA6D could improve OA in rat OA models, as indicated by H&E and Safranin O-Fast green staining, and also CT analysis. Further evaluation of SEMA6D suggested that SEMA6D promotes the anabolism and reduces the catabolism of C28/I2 chondrocytes via inhibiting the activation of the p38 pathway. The present research illustrated that SEMA6D is a negatively regulatory factor of miR-7 and a pivotal mediator of catabolism and anabolism in C28/I2 chondrocytes. SEMA6D exerts its function via inhibiting the activation of the p38 pathway.
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http://dx.doi.org/10.1155/2022/9674221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9225841PMC
June 2022

Cognitive Impairment and Psychopathology Are Related to Plasma Oxidative Stress in Long Term Hospitalized Patients With Chronic Schizophrenia.

Front Psychiatry 2022 10;13:896694. Epub 2022 Jun 10.

Department of Psychiatry, Institute of Mental Health, Suzhou Psychiatric Hospital, The Affiliated Guangji Hospital of Soochow University, Suzhou, China.

Background: The present study aimed to examine whether plasma oxidative stress is associated with cognitive impairment in long term hospitalized patients with chronic schizophrenia.

Method: Ninety-six chronic schizophrenia patients and 94 healthy unaffected subjects were enrolled. Plasma markers of oxidative stress, including malondialdehyde (MDA), manganese superoxide dismutase (MnSOD), catalase (CAT), and glutathione peroxidase (GSH-Px), were measured. Psychiatric symptoms and cognitive function were assessed with the Positive and Negative Syndrome Scale (PANSS) and the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), respectively.

Results: Plasma MDA levels and MnSOD and GSH-Px activities were significantly lower in schizophrenia patients than in healthy controls ( < 0.001), while plasma CAT activity was higher than in healthy controls ( < 0.005). Cognitive scores on the RBANS and all of its five subscales (all < 0.001) were significantly lower in schizophrenia patients than in healthy unaffected subjects. CAT and GSH-Px activities were positively correlated with the cognitive function scores corresponding to Visuospatial/Constructional abilities in the patient group ( = 0.298, 0.213, respectively, < 0.05). Also, the multiple regression analysis revealed that CAT and GSH-Px activities were independent and separate contributors to the Visuospatial/Constructional index of the RBANS. Meanwhile, CAT activity was negatively correlated with general pathological symptoms ( = -0.307, Bonferroni corrected = 0.008) and the total score of the PANSS domains ( = -0.299, Bonferroni corrected = 0.012).

Conclusion: Our results that the reduced of MDA level and the increased CAT activity in plasma in male patients with chronic schizophrenia suggest that redox imbalance may be associated with the pathophysiology of schizophrenia, and it can induce impaired cognition and psychiatric symptoms.
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http://dx.doi.org/10.3389/fpsyt.2022.896694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226302PMC
June 2022

CLSTN3 gene variant associates with obesity risk and contributes to dysfunction in white adipose tissue.

Mol Metab 2022 Jun 23:101531. Epub 2022 Jun 23.

Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai, China; Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Diabetes Institute, Shanghai, China. Electronic address:

Objective: White adipose tissue (WAT) possesses the remarkable remodeling capacity, and maladaptation of this ability contributes to the development of obesity and associated comorbidities. Calsyntenin-3 (CLSTN3) is a transmembrane protein that promotes synapse development in brain. Even though this gene has been reported to be associated with adipose tissue, its role in the regulation of WAT function is unknown yet. We aim to further assess the expression pattern of CLSTN3 gene in human adipose tissue, and investigate its regulatory impact on WAT function.

Methods: In our study, we observed the expression pattern of Clstn3/CLSTN3 gene in mouse and human WAT. Genetic association study and expression quantitative trait loci analysis were combined to identify the phenotypic effect of CLSTN3 gene variant in humans. This was followed by mouse experiments using adeno-associated virus-mediated human CLSTN3 overexpression in inguinal WAT. We investigated the effect of CLSTN3 on WAT function and overall metabolic homeostasis, as well as the possible underlying molecular mechanism.

Results: We observed that CLSTN3 gene was routinely expressed in human WAT and predominantly enriched in adipocyte fraction. Furthermore, we identified that the variant rs7296261 in the CLSTN3 locus was associated with a high risk of obesity, and its risk allele was linked to an increase in CLSTN3 expression in human WAT. Overexpression of CLSTN3 in inguinal WAT of mice resulted in diet-induced local dysfunctional expansion, liver steatosis, and systemic metabolic deficiency. In vivo and ex vivo lipolysis assays demonstrated that CLSTN3 overexpression attenuated catecholamine-stimulated lipolysis. Mechanistically, CLSTN3 could interact with amyloid precursor protein (APP) in WAT and increase APP accumulation in mitochondria, which in turn impaired adipose mitochondrial function and promoted obesity.

Conclusion: Taken together, we provide the evidence for a novel role of CLSTN3 in modulating WAT function, thereby reinforcing the fact that targeting CLSTN3 may be a potential approach for the treatment of obesity and associated metabolic diseases.
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http://dx.doi.org/10.1016/j.molmet.2022.101531DOI Listing
June 2022

Targeting miR-30d reverses pathological cardiac hypertrophy.

EBioMedicine 2022 Jun 22;81:104108. Epub 2022 Jun 22.

Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Nantong 226011, China; Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, Shanghai Engineering Research Center of Organ Repair, School of Life Science, Shanghai University, Shanghai 200444, China. Electronic address:

Background: Pathological cardiac hypertrophy occurs in response to numerous stimuli and precedes heart failure (HF). Therapies that ameliorate pathological cardiac hypertrophy are highly needed.

Methods: The expression level of miR-30d was analyzed in hypertrophy models and serum of patients with chronic heart failure by qRT-PCR. Gain and loss-of-function experiments of miR-30d were performed in vitro. miR-30d gain of function were performed in vivo. Bioinformatics, western blot, luciferase assay, qRT-PCR, and immunofluorescence were performed to examine the molecular mechanisms of miR-30d.

Findings: miR-30d was decreased in both murine and neonatal rat cardiomyocytes (NRCMs) models of hypertrophy. miR-30d overexpression ameliorated phenylephrine (PE) and angiotensin II (Ang II) induced hypertrophy in NRCMs, whereas the opposite phenotype was observed when miR-30d was downregulated. Consistently, the miR-30d transgenic rat was found to protect against isoproterenol (ISO)-induced pathological hypertrophy. Mechanistically, methyltransferase EZH2 could promote H3K27me3 methylation in the promotor region of miR-30d and suppress its expression during the pathological cardiac hypertrophy. miR-30d prevented pathological cardiac hypertrophy via negatively regulating its target genes MAP4K4 and GRP78 and inhibiting pro-hypertrophic nuclear factor of activated T cells (NFAT). Adeno-associated virus (AAV) serotype 9 mediated-miR-30d overexpression exhibited beneficial effects in murine hypertrophic model. Notably, miR-30d was reduced in serum of patients with chronic heart failure and miR-30d overexpression could significantly ameliorate pathological hypertrophy in human embryonic stem cell-derived cardiomyocytes.

Interpretation: Overexpression of miR-30d may be a potential approach to treat pathological cardiac hypertrophy.

Funding: This work was supported by the grants from National Key Research and Development Project (2018YFE0113500 to J Xiao), National Natural Science Foundation of China (82020108002 to J Xiao, 81900359 to J Li), the grant from Science and Technology Commission of Shanghai Municipality (20DZ2255400 and 21XD1421300 to J Xiao, 22010500200 to J Li), Shanghai Sailing Program (19YF1416400 to J Li), the "Dawn" Program of Shanghai Education Commission (19SG34 to J Xiao), the "Chen Guang" project supported by the Shanghai Municipal Education Commission and Shanghai Education Development Foundation (19CG45 to J Li).
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http://dx.doi.org/10.1016/j.ebiom.2022.104108DOI Listing
June 2022

Exploring the possible molecular targeting mechanism of Saussurea involucrata in the treatment of COVID-19 based on bioinformatics and network pharmacology.

Comput Biol Med 2022 Jul 25;146:105549. Epub 2022 Apr 25.

School of Life Sciences, Shihezi University, Xiangyang Street, Shihezi, 832003, PR China. Electronic address:

Objective: Based on bioinformatics and network pharmacology, the treatment of Saussurea involucrata (SAIN) on novel coronavirus (COVID-19) was evaluated by the GEO clinical sample gene difference analysis, compound-target molecular docking, and molecular dynamics simulation. role in the discovery of new targets for the prevention or treatment of COVID-19, to better serve the discovery and clinical application of new drugs.

Materials And Methods: Taking the Traditional Chinese Medicine System Pharmacology Database (TCMSP) as the starting point for the preliminary selection of compounds and targets, we used tools such as Cytoscape 3.8.0, TBtools 1.098, AutoDock vina, R 4.0.2, PyMol, and GROMACS to analyze the compounds of SAIN and targets were initially screened. To further screen the active ingredients and targets, we carried out genetic difference analysis (n = 72) through clinical samples of COVID-19 derived from GEO and carried out biological process (BP) analysis on these screened targets (P ≤ 0.05)., gene = 9), KEGG pathway analysis (FDR≤0.05, gene = 9), protein interaction network (PPI) analysis (gene = 9), and compounds-target-pathway network analysis (gene = 9), to obtain the target Point-regulated biological processes, disease pathways, and compounds-target-pathway relationships. Through the precise molecular docking between the compounds and the targets, we further screened SAIN's active ingredients (Affinity ≤ -7.2 kcal/mol) targets and visualized the data. After that, we performed molecular dynamics simulations and consulted a large number of related Validation of the results in the literature.

Results: Through the screening, analysis, and verification of the data, it was finally confirmed that there are five main active ingredients in SAIN, which are Quercitrin, Rutin, Caffeic acid, Jaceosidin, and Beta-sitosterol, and mainly act on five targets. These targets mainly regulate Tuberculosis, TNF signaling pathway, Alzheimer's disease, Pertussis, Toll-like receptor signaling pathway, Influenza A, Non-alcoholic fatty liver disease (NAFLD), Neuroactive ligand-receptor interaction, Complement and coagulation cascades, Fructose and mannose metabolism, and Metabolic pathways, play a role in preventing or treating COVID-19. Molecular dynamics simulation results show that the four active ingredients of SAIN, Quercitrin, Rutin, Caffeic acid, and Jaceosidin, act on the four target proteins of COVID-19, AKR1B1, C5AR1, GSK3B, and IL1B to form complexes that can be very stable in the human environment. Tertiary structure exists.

Conclusion: Our study successfully explained the effective mechanism of SAIN in improving COVID-19, and at the same time predicted the potential targets of SAIN in the treatment of COVID-19, AKR1B1, IL1B, and GSK3B. It provides a new basis and provides great support for subsequent research on COVID-19.
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http://dx.doi.org/10.1016/j.compbiomed.2022.105549DOI Listing
July 2022

Primary clear cell adenocarcinoma of female urinary tract: a case report and literature review.

BMC Womens Health 2022 Jun 24;22(1):251. Epub 2022 Jun 24.

Department of Urology, Xiangtan Central Hospital, Xiangtan, 411100, Hunan, China.

Background: Primary clear cell adenocarcinoma of the urethra is extremely rare, reported only in single case reports, and its histological origin is not clear. There is no standard treatment for CCAU at present, and surgery is still the main treatment for CCAU without distant metastasis.

Case Presentation: A 67-year-old female complained of gross hematuria with frequent micturition and urgency. No urethral diverticulum was found by cystoscopy or MRI, and the mass grew around the urethra. Urethral and anterior pelvic viscera resection was performed. Clear cell adenocarcinoma was confirmed by immunohistochemistry after the operation, and no recurrence or metastasis was found after one year of follow-up.

Conclusion: CCAU is very rare, and most cases originate from the urethral diverticulum and some may also originate from tissues around the urethra. For CCAU patients without distant metastasis, the main treatment is still surgery, and radiotherapy and chemotherapy can be performed for patients with distant metastasis. Gene detection may provide guidance for the precise chemotherapy of CCAU.
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http://dx.doi.org/10.1186/s12905-022-01835-6DOI Listing
June 2022

Refractive Index and Temperature Sensing Performance of Microfiber Modified by UV Glue Distributed Nanoparticles.

Polymers (Basel) 2022 Jun 15;14(12). Epub 2022 Jun 15.

Shaanxi Engineering Research Center of Controllable Neutron Source, School of Electronic Information, Xijing University, Xi'an 710123, China.

Dielectric materials with high refractive index have been widely studied to develop novel photonic devices for modulating optical signals. In this paper, the microfibers were modified by silicon nanoparticles (NPs) and silver NPs mixed in UV glue with ultra-low refractive index, respectively, whose corresponding optical and sensing properties have been studied and compared. The influence from either the morphological parameters of microfiber or the concentration of NPs on the refractive index sensing performance of microfiber has been investigated. The refractive index sensitivities for the microfiber tapers elaborated with silver NPs and silicon NPs were experimentally demonstrated to be 1382.3 nm/RIU and 1769.7 nm/RIU, respectively. Furthermore, the proposed microfiber was encapsulated in one cut of capillary to develop a miniature temperature probe, whose sensitivity was determined as 2.08 nm/°C, ranging from 28 °C to 43 °C.
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http://dx.doi.org/10.3390/polym14122425DOI Listing
June 2022

Maternal High-Fat Diet Aggravates Allergic Asthma in Offspring via Modulating CD4 T-Cell Differentiation.

Nutrients 2022 Jun 16;14(12). Epub 2022 Jun 16.

Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200011, China.

Maternal improper nutrition has been reported to trigger respiratory disorders in offspring. Here, we characterized the effects of high-fat environment in the fetal period on mice and human cord blood CD4 T-lymphocytes, and investigated their roles in susceptibility to asthma. Mice born to mothers that consumed a high-fat diet (HFD) throughout the gestation period were sensitized by ovalbumin to establish an experimental asthma model. To further extrapolate to humans, we collected cord blood from neonates of hypercholesterolemic (HC) mothers ( = 18) and control mothers ( = 20). In mice, aggravated airway hyperresponsiveness and inflammation revealed that maternal high-fat diet could lead to exacerbated allergic asthma in adult offspring. It was partially due to augmented activation and proliferation of CD4 T-cells, where upregulated mRNA levels may be potentially involved. Notably, naïve HFD CD4 T-cells had enhanced T2-based immune response both in vivo and in vitro, resulting from DNA hypomethylation of the promoter region. Moreover, in human, T2 cytokines transcripts were enhanced in CD4 T-cells of the HC group, which was associated with an increased risk of developing allergic diseases at 3 years old. Together, our study indicated that early life improper nutrition-triggered epigenetic changes in T-cells may contribute to long-lasting alterations in allergic diseases.
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http://dx.doi.org/10.3390/nu14122508DOI Listing
June 2022

A Highly Efficient Bismuth Nitrate/Keto-ABNO Catalyst System for Aerobic Oxidation of Alcohols to Carbonyl Compounds under Mild Conditions.

Molecules 2022 Jun 9;27(12). Epub 2022 Jun 9.

National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Huaiyin Institute of Technology, Huaian 223003, China.

An efficient and practical catalytic system for the oxidation of alcohols to aldehydes/ketones using catalytic amounts of Bi(NO) and Keto-ABNO (9-azabicyclo [3.3.1]nonan-3-one -oxyl) with air as the environmentally benign oxidant was developed. Various primary and secondary alcohols were smoothly oxidized to the corresponding products under mild conditions, and satisfactory yields were achieved. Moreover, this methodology avoids the use of a ligand and base. The gram-scale reaction was demonstrated for the oxidation of 1-phenyl ethanol, and the product of acetophenone was obtained at an isolated yield of about 94%.
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http://dx.doi.org/10.3390/molecules27123727DOI Listing
June 2022

Effect of Sulfur Content in Sulfate-Rich Copper Tailings on the Properties of MgO-Activated Slag Materials.

Materials (Basel) 2022 Jun 20;15(12). Epub 2022 Jun 20.

School of Civil Engineering and Architecture, Anhui University of Science and Technology, Huainan 232001, China.

The high-value utilization of sulfate-rich tailings (SRCTs) can accelerate their mass consumption, so the many problems caused by the massive accumulation of SRCTs can be alleviated, such as environmental pollution, land occupation, security risk, etc. This study proposes using SRCTs to replace fine natural aggregates in MgO-activated slag materials (MASMs) and investigate the influence of the sulfur content in SRCTs on the properties of MASMs. The experimental results showed that the 28 d compressive strength of MASM mortars was increased by up to 83% using SRCT composites. Two major mechanisms were discovered: additional hydration product formation and pore structure refinement. The results of XRD suggested that incorporating SRCT composite into MASMs increased the production of expansive sulfate-containing hydration products, such as ettringite, gypsum, and hydroxyl-Afm. The results of element mapping showed that the oxidation of pyrite in SRCTs could release sulfates into the surrounding area and participate in the hydration of MASM, indicating that SRCTs can work as an auxiliary activator for MASMs. Furthermore, the addition of SRCT significantly refined the pore structure of MASMs, leading to the reduction in porosity by up to 37.77%. These findings confirm a synergistic effect on activating the slag between SRCTs and MgO, promoting the mass utilization of SRCTs. As a result, the additional expansive hydration products contribute to the enhanced compressive strength and refined pore structure.
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http://dx.doi.org/10.3390/ma15124340DOI Listing
June 2022

Study on Toughening and Temperature Sensitivity of Polyurethane Cement (PUC).

Materials (Basel) 2022 Jun 18;15(12). Epub 2022 Jun 18.

School of Civil Engineering, Shandong University, Jinan 250061, China.

Polyurethane cement (PUC) is now commonly used in the reinforcement of old bridges, which exhibit various issues such as poor toughness, temperature-sensitive mechanical properties, and brittle failure. These problems can lead to the failure of the reinforcement effect of the PUC on old bridges in certain operating environments, leading to the collapse of such reinforced bridges. In order to alleviate these shortcomings, in this study, the toughness of PUC is improved by adding polyvinyl alcohol (PVA) fiber, carbon fiber, and steel fiber. In addition, we study the change law of the flexural strength of PUC between -40 °C and +40 °C. The control parameters evaluated are fiber type, fiber volume ratio, and temperature. A series of flexural tests and scanning electron microscope (SEM) test results show that the flexural strength first increases and then decreases with the increase in the volume-doping ratio of the three fibers. The optimum volume-mixing ratios of polyvinyl alcohol (PVA) fiber, carbon fiber, and steel fiber are 0.3%, 0.04% and 1%, respectively. Excessive addition of fiber will affect the operability and will adversely affect the mechanical properties. The flexural strength of both fiber-reinforced and control samples decreases with increasing temperature. Using the flexural test results, a two-factor (fiber content, temperature) BP neural network flexural strength prediction model is established. It is verified that the model is effective and accurate, and the experimental value and the predicted value are in good agreement.
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http://dx.doi.org/10.3390/ma15124318DOI Listing
June 2022

Nonlinear Modeling Study of Aerodynamic Characteristics of an X38-like Vehicle at Strong Viscous Interaction Regions.

Entropy (Basel) 2022 Jun 17;24(6). Epub 2022 Jun 17.

Computational Aerodynamics Institute, China Aerodynamics Research and Development Center, Mianyang 621000, China.

Strong viscous interaction and multiple flow regimes exist when vehicles fly at high altitude and high Mach number conditions. The Navier-Stokes(NS) solver is no longer applicable in the above situation. Instead, the direct simulation Monte Carlo (DSMC) method or Boltzmann model equation solvers are usually needed. However, they are computationally more expensive than the NS solver. Therefore, it is of great engineering value to establish the aerodynamic prediction model of vehicles at high altitude and high Mach number conditions. In this paper, the hypersonic aerodynamic characteristics of an X38-like vehicle in typical conditions from 70 km to 110 km are simulated using the unified gas kinetic scheme (UGKS), which is applicable for all flow regimes. The contributions of pressure and viscous stress on the force coefficients are analyzed. The viscous interaction parameters, Mach number, and angle of attack are used as independent variables, and the difference between the force coefficients calculated by UGKS and the Euler solver is used as a dependent variable to establish a nonlinear viscous interaction model between them in the range of 70-110 km. The evaluation of the model is completed using the correlation coefficient and the relative orthogonal distance. The conventional viscous interaction effect and rarefied effect are both taken into account in the model. The model can be used to quickly obtain the hypersonic aerodynamic characteristics of X38-like vehicle in a wide range, which is meaningful for engineering design.
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http://dx.doi.org/10.3390/e24060836DOI Listing
June 2022

CB1R-stabilized NLRP3 inflammasome drives antipsychotics cardiotoxicity.

Signal Transduct Target Ther 2022 Jun 24;7(1):190. Epub 2022 Jun 24.

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences & Human Phenome Institute, Fudan University, Shanghai, 200438, China.

Long-term use of antipsychotics is a common cause of myocardial injury and even sudden cardiac deaths that often lead to drug withdrawn or discontinuation. Mechanisms underlying antipsychotics cardiotoxicity remain largely unknown. Herein we performed RNA sequencing and found that NLRP3 inflammasome-mediated pyroptosis contributed predominantly to multiple antipsychotics cardiotoxicity. Pyroptosis-based small-molecule compound screen identified cannabinoid receptor 1 (CB1R) as an upstream regulator of the NLRP3 inflammasome. Mechanistically, antipsychotics competitively bond to the CB1R and led to CB1R translocation to the cytoplasm, where CB1R directly interacted with NLRP3 inflammasome via amino acid residues 177-209, rendering stabilization of the inflammasome. Knockout of Cb1r significantly alleviated antipsychotic-induced cardiomyocyte pyroptosis and cardiotoxicity. Multi-organ-based investigation revealed no additional toxicity of newer CB1R antagonists. In authentic human cases, the expression of CB1R and NLRP3 inflammasome positively correlated with antipsychotics-induced cardiotoxicity. These results suggest that CB1R is a potent regulator of the NLRP3 inflammsome-mediated pyroptosis and small-molecule inhibitors targeting the CB1R/NLRP3 signaling represent attractive approaches to rescue cardiac side effects of antipsychotics.
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http://dx.doi.org/10.1038/s41392-022-01018-7DOI Listing
June 2022

Robust, Healable, Self-Locomotive Integrated Robots Enabled by Noncovalent Assembled Gradient Nanostructure.

Nano Lett 2022 Jun 22. Epub 2022 Jun 22.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute, Sichuan University, Chengdu 610065, China.

Integration, being lightweight, and intelligence are important orientations for the future advancement of soft robots. However, existing soft robots are generally hydrogels or silicone rubber, which are inherently mechanically inferior and easily damaged and difficult to integrate functions. Here, inspired by nacre, an elastomer actuator with sulfonated graphene-based gradient nanostructures is constructed via supramolecular multiscale assembly. The resulting nanocomposite possesses an ultrahigh toughness of 141.19 MJ/m and high room-temperature self-healing efficiency (89%). The proof-of-concept robot is demonstrated to emphasize its maximum swimming speed of 2.67 body length per second, whose speed is comparable to that of plankton, representing the outperformance of most artificial soft robots. Furthermore, the robot can stably absorb pollutants and recover its robustness and functionality even when damaged. This study breaks the mutual exclusivity of functional execution and fast locomotions, and we anticipate that our nanostructural design will offer an effective extended path to other integrated robots that required multifunction integration.
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http://dx.doi.org/10.1021/acs.nanolett.2c01375DOI Listing
June 2022

[Characterization of greenhouse gas emissions and stable isotopic composition of ammonia during industrial composting process].

Ying Yong Sheng Tai Xue Bao 2022 Jun;33(6):1451-1458

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

Carbon dioxide (CO), nitrous oxide (NO), methane (CH) and ammonia (NH) emitted during the composting of livestock and poultry waste are important gaseous atmospheric pollutants. However, most previous studies on compost-related anthropogenic emissions of these gases were based on small reactor composting. Our understanding of their emissions during industrial composting remains extremely limited. In order to explore the influence of gas produced by industrial composting on regional environment, we monitored CO, CH, NO and NH emissions during industrial composting for 19 days and characterized the isotopic composition of emitted NH. On average, the emission rates of CO, CH, NO, and NH during the composting cycle were 86.8 g CO-C·d·m, 9.8 g CH-C·d·m, 3.7 mg NO-N·d·m and 736.6 mg NH-N·d·m, respectively. The contribution of CH to daily global warming potential (GWP) was the highest (65%), followed by CO, NH(indirect), and NO. Moreover, ammonia emitted from industrial compost had a mean δN value of -11.6‰±1.2‰ (range: -21.8‰--7.2‰). Overall, this study provided useful information for understanding greenhouse gas emission dynamics and characterizing atmospheric NH sources during composting process in livestock and poultry breeding areas.
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http://dx.doi.org/10.13287/j.1001-9332.202206.009DOI Listing
June 2022

Development of a novel signature derived from single cell RNA-sequencing for preoperative prediction of lymph node metastasis in head and neck squamous cell carcinoma.

Head Neck 2022 Jun 21. Epub 2022 Jun 21.

Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital, Nanjing Medical University, Nanjing, China.

Background: Lymph node metastasis (LNM) is considered as an adverse prognostic indicator for cancer patients. Preoperative knowledge of LNM is valuable for pretreatment decision making. Here, we sought to develop and validate an LNM signature for preoperative prediction of LNM in patients with head and neck squamous cell carcinoma (HNSCC).

Methods: By studying single cell RNA-sequencing data (scRNA-seq), differentially expressed mRNA were selected and analyzed through univariate logistic regression and least absolute shrinkage and selection operator (LASSO) to identify an LNM signature. Multivariate logistic regression was utilized to establish an LNM nomogram incorporating LNM signature and T-classification.

Results: The LNM signature was significantly associated with lymph node status and prognosis. The LNM signature and LNM nomogram displayed a robust predictive effect.

Conclusion: Our study reveals that LNM signature is a powerful biomarker for preoperative prediction of LNM in patients with HNSCC, which may be effective to realize individualized outcome prediction.
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http://dx.doi.org/10.1002/hed.27126DOI Listing
June 2022

[Synthesis, characterization, and immunological activity of Rehmannia glutinosa seleno-polysaccharides].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(11):2938-2946

School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing 211198, China.

The present study explored the optimum synthesis process of Rehmannia glutinosa seleno-polysaccharides with acetic acid as a catalyst, characterized the structure of R. glutinosa seleno-polysaccharides by Fourier transform infrared spectroscopy(FT-IR), scanning electron microscopy(SEM), thermogravimetry(TG), and atomic force microscopy(AFM), and preliminarily investigated the immunological activity of R. glutinosa seleno-polysaccharides. The results showed that the optimal conditions for the synthesis of R. glutinosa seleno-polysaccharides included m(acetic acid)∶m(R. glutinosa polysaccharides)=0.80, m(Na_2SeO_3)∶m(R. glutinosa polysaccharides)=1.25, reaction temperature of 80.0 ℃, and reaction time of 7.0 h. Under these conditions, the selenium content of R. glutinosa seleno-polysaccharides was 2.239 mg·g~(-1). The acetic acid catalysis method was milder than the nitric acid method, without affecting the structure of polysaccharides. The results of IR, SEM, TG, and AFM showed that R. glutinosa seleno-polysaccharides were properly prepared. The results of immunological activity showed that compared with the control group, R. glutinosa seleno-polysaccharides could significantly promote the phagocytic capacity of mouse monocyte macrophages and improve the spleen index and thymus index of mice. In the concentration range of 15-240 μg·mL~(-1), the proliferation of spleen lymphocytes of mice was strengthened, and the IL-2 and IFN-γ secretion by Th1 cytokines was promoted. This study can provide references for the further development and application of R. glutinosa polysaccharides.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220224.304DOI Listing
June 2022

Sex Cord-Stromal Tumors of Testis: A Clinicopathologic and Follow-Up Study of 15 Cases in a High-Volume Institute of China.

Front Med (Lausanne) 2022 31;9:816012. Epub 2022 May 31.

Department of Urology, West China Hospital, Institute of Urology, Sichuan University, Chengdu, China.

Objectives: To report the first series of testicular sex cord-stromal tumors (TSCSTs) with detailed clinicopathologic findings and long-term follow-up in the Chinese population.

Patients And Methods: From 2008 to 2018, 15 patients with TSCST were included in our study. The tumors were analyzed for epidemiological parameters, clinical characteristics, tumor markers, therapy, and follow-up data.

Results: The median age of the patients was 28 years (range, 13-80 years). Para-aortic lymph node metastases were detected in 2 patients after radiological evaluation. Orchiectomy was performed in all patients, and the median diameter of the tumor was 1.5 cm (range, 0.5-5.0 cm). Nine Leydig cell tumors (LCTs), 5 Sertoli cell tumors (SCTs), and 1 unclassified type were confirmed after pathologic evaluation. Thirteen patients (86.7%) were categorized as stage I, and 2 patients (13.3%) were categorized as stage II. The median clinical follow-up was 39.0 months (range, 5-97 months), which showed 10 alive patients, such as 1 patient with progression at 40 months after orchiectomy. The 3- and 5-year progression-free survivals were 100 and 90.0%, respectively.

Conclusion: Testicular sex cord-stromal tumor at stages I and II is a rare subtype with benign behavior and a favorable prognosis in the Chinese population. However, lymph node metastases may be the dominant risk factor for patients with TSCST.
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http://dx.doi.org/10.3389/fmed.2022.816012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197338PMC
May 2022

Erratum: Overexpression of TC2N is associated with poor prognosis in gastric cancer: Erratum.

J Cancer 2022 25;13(8):2413. Epub 2022 Apr 25.

Department of Gastrointestinal Surgery, the First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2nd Road, Guangzhou 510080, China.

[This corrects the article DOI: 10.7150/jca.50653.].
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http://dx.doi.org/10.7150/jca.73345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174853PMC
April 2022

Improved polar-code-based efficient post-processing algorithm for quantum key distribution.

Sci Rep 2022 Jun 16;12(1):10155. Epub 2022 Jun 16.

School of Computer Science and Technology, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, China.

Combined with one-time pad encryption scheme, quantum key distribution guarantees the unconditional security of communication in theory. However, error correction and privacy amplification in the post-processing phase of quantum key distribution result in high time delay, which limits the final secret key generation rate and the practicability of quantum key distribution systems. To alleviate this limitation, this paper proposes an efficient post-processing algorithm based on polar codes for quantum key distribution. In this algorithm, by analyzing the channel capacity of the main channel and the wiretap channel respectively under the Wyner's wiretap channel model, we design a codeword structure of polar codes, so that the error correction and privacy amplification could be completed synchronously in a single step. Through combining error correction and privacy amplification into one single step, this efficient post-processing algorithm reduces complexity of the system and lower the post-processing delay. Besides, the reliable and secure communicaiton conditions for this algorithm has been given in this paper. Simulation results show that this post-processing algorithm satisfies the reliable and secure communication conditions well.
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http://dx.doi.org/10.1038/s41598-022-14145-6DOI Listing
June 2022

Cold-Adaptive Evolution at the Reproductive Stage in Geng/Japonica Subspecies Reveals the role of OsMAPK3 and OsLEA9.

Plant J 2022 Jun 15. Epub 2022 Jun 15.

State Key Laboratory of Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.

Cold stress at the reproductive stage severely affects production and geographic distribution of rice. The Geng/japonica subpopulation gradually developed stronger cold adaptation than the Xian/indica subpopulation during the long-term domestication of cultivated rice. However, the evolutionary path and natural alleles underlying the cold adaptability of intra-Geng subspecies remain largely unknown. Here, we identified MITOGEN-ACTIVATED PROTEIN KINASE 3 (OsMAPK3) and LATE EMBRYOGENESIS ABUNDANT PROTEIN 9 (OsLEA9) as two important regulators for cold adaptation of Geng subspecies from a combination of transcriptome analysis and genome-wide association study. Transgenic validation showed that OsMAPK3 and OsLEA9 confer cold tolerance at the reproductive stage. Selection and evolution analysis suggested that the Geng version of OsMAPK3 (OsMAPK3 ) directly evolved from Chinese O. rufipogon III and was largely retained in high-latitude and high-altitude regions with low temperatures during domestication. Later, the functional nucleotide polymorphism (FNP-776) in the KMXBG & LJXHG version of OsLEA9 (OsLEA9 ) promoter originated from novel variation of intra-Geng was selected and predominantly retained in temperate Geng to improve the adaptation of Geng together with OsMAPK3 to colder climatic conditions in high-latitude areas. Breeding potential analysis suggested that pyramiding of OsMAPK3 and OsLEA9 enhanced the cold tolerance of Geng and promote the expansion of cultivated rice to colder regions. This study not only highlights the evolutionary path taken by the cold-adaptive differentiation of intra-Geng, but also provides new genetic resources for rice molecular breeding in low-temperature areas.
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http://dx.doi.org/10.1111/tpj.15870DOI Listing
June 2022

Maturation of induced pluripotent stem cell-derived cardiomyocytes and its therapeutic effect on myocardial infarction in mouse.

Bioact Mater 2023 Feb 4;20:286-305. Epub 2022 Jun 4.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, China.

Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have an irreplaceable role in the treatment of myocardial infarction (MI), which can be injected into the transplanted area with new cardiomyocytes (Cardiomyocytes, CMs), and improve myocardial function. However, the immaturity of the structure and function of iPSC-CMs is the main bottleneck at present. Since collagen participates in the formation of extracellular matrix (ECM), we synthesized nano colloidal gelatin (Gel) with collagen as the main component, and confirmed that the biomaterial has good biocompatibility and is suitable for cellular in vitro growth. Subsequently, we combined the PI3K/AKT/mTOR pathway inhibitor BEZ-235 with Gel and found that the two combined increased the sarcomere length and action potential amplitude (APA) of iPSC-CMs, and improved the Ca processing ability, the maturation of mitochondrial morphological structure and metabolic function. Not only that, Gel can also prolong the retention rate of iPSC-CMs in the myocardium and increase the expression of Cx43 and angiogenesis in the transplanted area of mature iPSC-CMs, which also provides a reliable basis for the subsequent treatment of mature iPSC-CMs.
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http://dx.doi.org/10.1016/j.bioactmat.2022.05.024DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167678PMC
February 2023

Pharmacokinetics and pharmacodynamic interaction of bergamottin with atorvastatin in rats.

Xenobiotica 2022 Jun 28:1-5. Epub 2022 Jun 28.

Department of Pharmacy, Huabei Petroleum Administration Bureau General Hospital, Cangzhou, Hebei, China.

1. The pharmacokinetics and pharmacodynamic of concomitant administration of atorvastatin with bergamottin were investigated perspectives to reveal the potential herb-drug interaction between these two drugs.2. The hyperlipidaemia-induced Wistar rats received atorvastatin with or without bergamottin (2.5 mg/kg). The concentration of atorvastatin in the rats' serum was determined using an established HPLC/MS/MS method. The pharmacokinetic parameters were calculated using DAS software. Lipid levels were determined.3. Bergamottin increases the C (from 48 ± 5 ng/mL to 89 ± 7 ng/mL), AUC (from 176 ± 27 to 552 ± 131 h∗μg/L), and the elimination half-life of atorvastatin (t) of atorvastatin. Co-administration of atorvastatin with bergamottin decreased total cholesterol (by 14%), low-density lipoproteins-cholesterol (by 20%), and triglyceride (by 12%), but increased thigh-density lipoprotein-cholesterol, when compared with atorvastatin alone.4. Co-administration of bergamottin and atorvastatin alters both pharmacokinetics and pharmacodynamics of atorvastatin. This study provides pre-clinical information evidence that bergamottin could potentiate the therapeutic efficacy of atorvastatin or increase its accumulation and adverse effects.
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http://dx.doi.org/10.1080/00498254.2022.2090301DOI Listing
June 2022

Engineered Stable Bioactive Per Se Amphiphilic Phosphorus Dendron Nanomicelles as a Highly Efficient Drug Delivery System To Take Down Breast Cancer In Vivo.

Biomacromolecules 2022 Jun 13. Epub 2022 Jun 13.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.

Conventional small molecular chemical drugs always have challenging limitations in cancer therapy due to their high systemic toxicity and low therapeutic efficacy. Nanotechnology has been applied in drug delivery, bringing new promising potential to realize effective cancer treatment. In this context, we develop here a new nanomicellar drug delivery platform generated by amphiphilic phosphorus dendrons (1-C17G3.HCl), which could form micelles for effective encapsulation of a hydrophobic anticancer drug doxorubicin (DOX) with a high drug loading content (42.4%) and encapsulation efficiency (96.7%). Owing to the unique dendritic rigid structure and surface hydrophilic groups, large steady void space of micelles can be created for drug encapsulation. The created DOX-loaded micelles with a mean diameter of 26.3 nm have good colloidal stability. Strikingly, we show that the drug-free micelles possess good intrinsic anticancer activity and act collectively with DOX to take down breast cancer cells in vitro and the xenografted tumor model in vivo through upregulation of Bax, PTEN, and p53 proteins for enhanced cell apoptosis. Meanwhile, the resulting [email protected] micelles significantly abolish the toxicity relevant to the free drug. The findings of this study demonstrate a unique nanomicelle-based drug delivery system created with the self-assembling amphiphilic phosphorus dendrons that may be adapted for chemotherapy of different cancer types.
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http://dx.doi.org/10.1021/acs.biomac.2c00197DOI Listing
June 2022
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