Publications by authors named "Jin Li"

3,837 Publications

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High Prevalence of Preexisting HBV Polymerase Mutations in Pregnant Women Does Not Limit the Antiviral Therapy Efficacy.

Can J Infect Dis Med Microbiol 2021 19;2021:6653546. Epub 2021 Apr 19.

Clinical Research Center, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.

Background: HBV-resistant mutants in treatment-naïve patients may lead to antiviral treatment failure. It is not clear if HBV mutants are present in pregnant women and about the influence of the preexisting mutants on the short-term antiviral therapy during pregnancy.

Method: We enrolled 73 pregnant women with high HBV DNA load and telbivudine (TBV) treatment during pregnancy in this retrospective study. The UDPS was used to detect the HBV mutations before and after the TBV treatment.

Results: Before TBV treatment, the complexity of HBV quasispecies of all subjects was 0.40 ± 0.09; 41.1% (30/73) and 53.4% (39/73) subjects had rtM204I/V and rtN236 T/A detected, respectively; and 9.6% (7/73) patients had more than 20% frequency mutation of rtM204I/V, which was also similar with high frequency of rtN236 T/A mutation (41.1% vs. 53.4%, =0.136; frequencies >20%: 9.6% vs. 5.5%, =0.347). After TBV treatment, 71.2% (52/73) subjects had HBV DNA load ≥ 10 IU/mL at delivery. Among them, 75.0% of patients with rtM204I positive had HBV DNA load ≥10 IU/mL at delivery, which was comparable with the subjects without rtM204I (75.0% vs. 70.8%, =0.710). No changes were found in the frequencies and the complexity of HBV quasispecies of rtM204I mutation after the TVB treatment.

Conclusion: The prevalence of preexisting drug-resistant mutations among pregnant women was high using UPDS. However, the preexisting HBV mutation had limited influence on the efficacy of short-term TBV treatment, and TBV treatment during late pregnancy seemed not to increase the risk of emerging HBV-resistant mutants.
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http://dx.doi.org/10.1155/2021/6653546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079218PMC
April 2021

Phytohemagglutinin ameliorates HFD-induced obesity by increasing energy expenditure.

J Mol Endocrinol 2021 May 1. Epub 2021 May 1.

X Liu, Institute of Neuroscience and Translational Medicine, Zhoukou Normal University, Zhoukou, China.

Despite all modern advances in medicine, there are few reports of effective and safe drugs to treat obesity. Our objective was to screen anti-obesity natural compounds, and to verify whether they can reduce the body weight gain and investigate their molecular mechanisms. By using drug-screening methods, Phytohemagglutinin (PHA) was found to be the most anti-obesity candidate natural compound. Six-week-old C57BL/6J mice were fed with high-fat diet (HFD) and intraperitoneally injected with 0.25mg/kg PHA every day for 8 weeks. The body weight, glucose homeostasis, oxygen consumption and physical activity were assessed. We also measured the heat intensity, body temperature and the gene expression of key regulators of energy expenditure. Prevention study results showed PHA treatment not only reduced the body weight gain, but also maintained glucose homeostasis in HFD-fed mice. Further study indicated energy expenditure and uncoupling protein 1 (UCP-1) expression of brown adipose tissue (BAT) and white adipose tissue (WAT) in HFD-fed mice were significantly improved by PHA. In the therapeutic study, the similar effect was observed. PHA inhibited lipid droplet formation and up-regulated mitochondrial related genes expression during adipogenesis in vitro. UCP-1 KO mice displayed no differences in body weight, glucose homeostasis and core body temperature between PHA and control groups. Our results suggest that PHA prevent and treat obesity by increasing energy expenditure though up-regulation of BAT thermogenesis.
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http://dx.doi.org/10.1530/JME-20-0349DOI Listing
May 2021

Micro-opto-electro-mechanical gyroscope based on the Talbot effect of a single-layer near-field diffraction grating.

Appl Opt 2021 May;60(13):3724-3731

We demonstrate a novel, to the best of our knowledge, micro-opto-electro-mechanical system (MOEMS) gyroscope based on the Talbot effect of a single-layer near-field diffraction grating. The Talbot effect of an optical grating is studied both theoretically and experimentally. A structure of grating-mirror combination, fabricated by the micro-nano processing method, is used for out-of-plane structure detection. The detection of a weak Coriolis force is realized by using the highly sensitive displacement characteristic of Talbot imaging of near-field diffraction with a mirror mass block and single-layer grating. The experimental results show that, the micro-displacement detection sensitivity can reach up to 0.09%/nm, and the MOEMS gyroscope can be moved in the driven direction, with a resonant frequency of 7048 Hz and a quality factor of 700, which indicates great potential of the Talbot effect in developing novel high-performance micro-gyroscopes.
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http://dx.doi.org/10.1364/AO.420541DOI Listing
May 2021

Sacral dural arteriovenous fistula supplied by bilateral lateral sacral arteries: case report and literature review.

Br J Neurosurg 2021 May 13:1-4. Epub 2021 May 13.

Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, PR China.

Sacral dural arteriovenous fistulas (DAVFs) with bilateral arterial supplies are extremely rare. To date, only two cases with arterial supply from bilateral lateral sacral arteries (LSAs) were reported. We report a rare case of sacral DAVF with arterial supply from bilateral LSAs. A 56-year-old man presented with a 2-month history of progressive weakness and numbness in his lower extremities, along with urinary incontinence. Spinal magnetic resonance imaging (MRI) showed extensive edema of the spinal cord, vascular flow voids, and intraparenchymal enhancement. Spinal angiography revealed a spinal DAVF at the level of S1 supplied by bilateral LSAs and drained ascending into the perimedullary venous plexus. The fistula was successfully treated with endovascular embolization. Sacral DAVFs present various diagnostic and treatment difficulties because of the complex angioarchitecture. Successful management of these lesions requires a profound understanding of the variable patterns of arterial supply in this region.
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http://dx.doi.org/10.1080/02688697.2021.1916434DOI Listing
May 2021

Plasma homocysteine and macular thickness in older adults-the Rugao Longevity and Aging Study.

Eye (Lond) 2021 May 11. Epub 2021 May 11.

State Key Laboratory of Genetic Engineering and MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China.

Objectives: To determine the association of plasma homocysteine levels with retinal layer thickness in a large community cohort of older adults.

Methods: The Rugao Longevity and Ageing Study is an observational, prospective and community-based cohort study. A total of 989 older adults who underwent spectral-domain optical coherence tomography (SD-OCT) were included and analyzed. Foveal, macular retinal nerve fibre layer (mRNFL) and ganglion cell layer plus inner plexiform layer (GC-IPL) thicknesses were measured by SD-OCT. Plasma homocysteine levels were measured using chemiluminescence immunoassay. Linear regression analyses were performed to evaluate the relationship between plasma homocysteine and retinal layer thickness while controlling for confounding factors.

Results: Of the 989 participants, 500 (50.56%) were men. The mean age was 78.26 (4.58) years, and the mean plasma homocysteine level was 16.38 (8.05) μmol/L. In multivariable analyses, each unit increase in plasma homocysteine was associated with an 8.84 × 10 (95% CI: -16.54 × 10 to -1.15 × 10, p = 0.032) μm decrease in the average inner thickness of the GC-IPL after controlling for confounding factors. The association remained significant even in participants without major cardiovascular disease or diabetes (β = -10.33 × 10, 95% CI: -18.49 × 10 to -2.18 × 10, p = 0.013). No significant associations of plasma homocysteine levels with macular thickness or mRNFL were found in primary and sensitivity analyses (p > 0.05).

Conclusions: Increased plasma homocysteine levels are associated with a thinner GC-IPL. Plasma homocysteine may be a risk factor for thinner retinas in older adults.
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http://dx.doi.org/10.1038/s41433-021-01549-3DOI Listing
May 2021

Unraveling the nitrogen removal properties and microbial characterization of "Candidatus Scalindua"-dominated consortia treating seawater-based wastewater.

Sci Total Environ 2021 May 1;786:147470. Epub 2021 May 1.

Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-environmental Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100085, China.

"Candidatus Scalindua", as known as marine anammox bacteria (MAB), was engineered to remove nitrogen from seawater-based wastewater (SWW). In this study, "Candidatus Scalindua" was successfully enriched within 106 days with marine sediments as inoculated sludge. The operating temperature was 20 ± 2 °C, and influent pH was 7.5 ± 0.1. Ammonia (NH-N) removal rate (ARR) was 0.53 kg/(m·d) with the NH-N loading rate of 0.68 kg/(m·d), and nitrite (NO-N) removal rate (NRR) was 0.57 kg/(m·d) at 0.89 kg/(m·d) NO-N loading rate. Nitrogen removal was negatively affected at an influent NO above 224 mg/L, which decreased the ARR and NRR to 0.36 and 0.31 kg/(m·d), respectively. The genus "Ca. Scalindua" dominated the reactor, and it synergistically coexisted with Marinicella to achieve efficient nitrogen removal. This work would help to better understand the nitrogen removal properties and microbial characterization of MAB in SWW wastewater treatment under low temperature.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147470DOI Listing
May 2021

[Analysis and Enlightenment of Foreign Open Breast Image Datasets].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Apr;43(2):271-277

Clinical Specimen Center,Chinese PLA General Hospital,Beijing 100853,China.

With the upsurge of medical artificial intelligence,the use of computer vision technology to study medical images,which can effectively help doctors to identify and screen diseases,has become a focus of researchers.This paper summarizes the basic situation,specific information,related research,and data sharing and utilizing ways of foreign breast image datasets.This review provides inspirations for the opening of Chinese medical and health data.
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http://dx.doi.org/10.3881/j.issn.1000-503X.12006DOI Listing
April 2021

Comparison of two methods in the treatment of congenital pseudarthrosis of clavicle: multicenter experience.

J Orthop Surg Res 2021 May 8;16(1):301. Epub 2021 May 8.

Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Background: Congenital pseudoarthrosis of the clavicle (CPC) is an uncommon entity. Owing to its scarce presentation, treatment of this disorder has not been well established. This study aimed (1) to compare surgical treatment methods that included excision of pseudoarthrosis and iliac crest bone graft and fixate with either the elastic stable intramedullary nail (ESIN) or K-wires or plate and screws, and (2) to assess the clinical outcomes of two different surgical methods.

Methods: A multi-central retrospective study was performed between 2013 and 2017 in four tertiary teaching hospitals. Fifteen clavicles of 11 children were identified as CPC. All patients underwent pseudarthrosis resection and iliac crest bone autograft. They were divided into two groups as per the surgical treatment they underwent-plate stabilization as group A and elastic stable intramedullary nailing (ESIN) or K-wires as group B. Nine clavicles in 6 patients in group A and 6 clavicles in 5 patients in group B, were included. The Quick Disabilities of the Arm and Shoulder (QuickDASH) score was used to assess patients' satisfaction and function following treatment at each follow-up.

Results: There were eight boys and three girls, with an average age of 4.7 years. All patients, except one with intellectual impairments, had radiological healing. Implant removal time was significantly shorter in group B compared to group A. No statistically significant differences existed in terms of age at surgery, time of radiological healing, complication, and clinical outcome between different groups.

Conclusion: Surgical resection of pseudoarthrosis with an iliac crest bone graft was an effective means of surgical treatment in CPC. However, ESIN or K-wires can achieve shorter union time compared to the plate. Hence, surgical treatment is recommended for congenital pseudarthrosis of clavicular in pediatric patients.

Level Of Evidence: Retrospective comparative study; Level III.
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http://dx.doi.org/10.1186/s13018-021-02438-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106166PMC
May 2021

Comparative efficacy and acceptability of antidepressants and benzodiazepines for the treatment of panic disorder: A systematic review and network meta-analysis.

Asian J Psychiatr 2021 May 3;60:102664. Epub 2021 May 3.

School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.

Objective: This systematic review aims to assess the efficacy and acceptability of the different types of antidepressants and benzodiazepines for the treatment of panic disorder (PD) in adult patients.

Methods: PubMed, Web of Science, EMBASE, MEDLINE, the Cochrane Library, and ClinicalTrials.gov were searched for randomized controlled trials (RCTs) published between 1995 and 2020 on the use of antidepressants and benzodiazepines for the treatment of PD. A systematic review and network meta-analysis were performed.

Results: 42 RCTs were included in the network meta-analysis, with a comparison of 11 interventions.Escitalopram (odds ratios OR 1.52, 95 % credible interval CI 1.09-2.10), venlafaxine (OR 1.33, 95 % CI 1.16-1.51) and benzodiazepines (OR 1.50, 95 % CI 1.29-1.75) had greater efficacy and acceptability than the placebo. Imipramine(OR 1.43, 95 % CI 1.15-1.79) was also demonstrated to be efficacious and tolerated but the results were restricted to small sample size. Moreover, paroxetine, sertraline, fluoxetine, citalopram and clomipramine (OR 1.37, 1.36, 1.45, 1.33 and 1.36, respectively) were more efficacious, although the acceptability of paroxetine and sertraline were significantly less tolerated than benzodiazepines. Notably, the efficacy of reboxetine and fluvoxamine were merely as equal as that of the placebo.

Outcomes: This is the first systematic review of antidepressants and benzodiazepines for the treatment of PD to use a network analysis. Escitalopram and venlafaxine as well as benzodiazepines may be effective choices as treatments for PD with relatively good acceptability, which still needs to be confirmed byhigh-quality RCTs.
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http://dx.doi.org/10.1016/j.ajp.2021.102664DOI Listing
May 2021

Immunogenicity and safety of 7-valent pneumococcal conjugate vaccine (PCV7) in children aged 2-5 years in China.

Vaccine 2021 May 5. Epub 2021 May 5.

Experimental Center for Basic Medical Teaching, Capital Medical University, Beijing, China. Electronic address:

Background: The widespread use of pneumococcal conjugate vaccines (PCVs) has significantly decreased pneumococcal disease worldwide. However, China has not adopted PCVs in their national immunization schedules and had only approved these vaccines for children aged 2-15 months by 2020.

Methods: In an open-label trial, enrolled healthy children aged 2-5 years old were randomized 1:1 and divided into a 7-valent pneumococcal conjugate vaccine (PCV7) group and a Haemophilus influenzae type b conjugate vaccine (Hib) group. Children in the PCV7 group received a single dose of PCV7, and the Hib group received a single dose of Hib vaccine. Blood samples were collected before and 6 months after vaccination. Immunogenicity and safety of PCV7 were assessed at prespecified time points.

Results: Six months after a single dose of PCV7, children in the PCV7 group for all 7 serotypes, IgG mean concentrations (GMCs) and opsonophagocytic geometric mean titres (GMTs) were significantly higher (P < .001) than at baseline, and the proportion of IgG ≥ 0.35 µg/mL ranged from 90.0% to 100%. Although the antibody level increased with age, preexisting antibodies did not induce hyporesponsiveness to PCV7. In the Hib group, the antibody levels were not significantly different or had changed slightly at 6 months. PCV7 was well tolerated in all age groups, and no serious adverse events (AEs) emerged during this study.

Conclusions: A single dose of PCV7 was immunogenic and safe for Chinese children aged 2-5 years, and the preexisting antibodies against the PCV7 serotypes did not change the response to vaccination. The findingssupported the effectiveness of PCV7 in this age group. PCVs with broader serotype coverage are expected to expand pneumococcal disease protection.
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http://dx.doi.org/10.1016/j.vaccine.2021.04.037DOI Listing
May 2021

Study of Real-Valued Distance Prediction for Protein Structure Prediction with Deep Learning.

Authors:
Jin Li Jinbo Xu

Bioinformatics 2021 May 7. Epub 2021 May 7.

Toyota Technological Institute at Chicago, USA.

Motivation: Inter-residue distance prediction by deep ResNet (convolutional residual neural network) has greatly advanced protein structure prediction. Currently the most successful structure prediction methods predict distance by discretizing it into dozens of bins. Here we study how well real-valued distance can be predicted and how useful it is for 3D structure modeling by comparing it with discrete-valued prediction based upon the same deep ResNet.

Results: Different from the recent methods that predict only a single real value for the distance of an atom pair, we predict both the mean and standard deviation of a distance and then fold a protein by the predicted mean and deviation. Our findings include: 1) tested on the CASP13 FM (free-modeling) targets, our real-valued distance prediction obtains 81% precision on top L/5 long-range contact prediction, much better than the best CASP13 results (70%); 2) our real-valued prediction can predict correct folds for the same number of CASP13 FM targets as the best CASP13 group, despite generating only 20 decoys for each target; 3) our method greatly outperforms a very new real-valued prediction method DeepDist in both contact prediction and 3D structure modeling; and 4) when the same deep ResNet is used, our real-valued distance prediction has 1-6% higher contact and distance accuracy than our own discrete-valued prediction, but less accurate 3D structure models.

Availability And Implementation: https://github.com/j3xugit/RaptorX-3DModeling.

Supplementary Information: Supplementary data are available at Bioinformatics online.
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http://dx.doi.org/10.1093/bioinformatics/btab333DOI Listing
May 2021

Genetic Variants Increase Susceptibility to Diabetic Kidney Disease in Chinese Patients with Type 2 Diabetes: A Cross-Sectional Case Control Study.

Mediators Inflamm 2021 17;2021:5521050. Epub 2021 Apr 17.

Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Centre for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Few studies have illuminated the genetic role of T cell costimulatory molecule variants in diabetic kidney disease (DKD) susceptibility. We aimed to investigate the causal role of genetic polymorphisms in with DKD susceptibility in patients with T2DM. A total of 3253 patients with T2DM were recruited for genotyping: including 204 DKD patients and 371 controls in stage 1 and 819 DKD patients and 563 controls in stage 2; besides, 1296 T2DM patients were selected for the analysis of association between loci and DKD-related traits. A subset of 227 T2DM patients (118 patients with DKD and 109 patients without DKD) from the total population above were selected to assess serum soluble CD28 (sCD28) levels. Then, we performed a candidate gene association study to identify single-nucleotide polymorphisms (SNPs) associated with DKD susceptibility and further used those SNPs to perform Mendelian randomization analyses of serum sCD28 level and DKD susceptibility. Under additive genetic models, -rs3116494 (OR = 1.29 [95% CI 1.11, 1.51], = 0.0011) and -rs3850890 (OR = 1.16 [95% CI 1.02, 1.31], = 0.0283) were associated with DKD susceptibility adjusted for age, gender, body mass index (BMI), duration of diabetes, and HbA1c. -rs3116494 was associated with serum sCD28 level ( = 0.26 [95% CI 0.08, 0.44], = 0.0043). The Mendelian randomization analysis showed that -rs3116494 played a causal role in DKD by influencing serum sCD28 levels ( = 1.15 [95% CI 0.46, 1.83], = 0.0010). In conclusion, we identified that two novel SNPs, -rs3116494 and -rs3850890, were associated with DKD susceptibility. Using the Mendelian randomization analysis, our study provided evidence for a causal relationship between serum CD28 levels and DKD with T2DM in the Chinese population.
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http://dx.doi.org/10.1155/2021/5521050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075672PMC
April 2021

Melatonin attenuates hepatic ischemia-reperfusion injury in rats by inhibiting NF-κB signaling pathway.

Hepatobiliary Pancreat Dis Int 2021 Apr 18. Epub 2021 Apr 18.

Organ Transplantation Center, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, China. Electronic address:

Background: The sterile inflammatory response is one of the key mechanisms leading to hepatic ischemia-reperfusion injury. Melatonin has been shown to prevent organ injuries, but its roles in the inflammatory response after hepatic ischemia-reperfusion injury have not been fully explored, especially in late ischemia-reperfusion injury. The present study aimed to investigate the roles and possible mechanisms of melatonin in the inflammatory response after hepatic ischemia-reperfusion injury.

Methods: Sixty Sprague-Dawley rats were randomly divided into a sham group, ischemia-reperfusion injury group (I/R group), and melatonin-treated group (M + I/R group). The rats in the I/R group were subjected to 70% hepatic ischemia for 45 min, followed by 5 or 24 h of reperfusion. The rats in the M + I/R group were injected with melatonin (10 mg/kg, intravenous injection) 15 min prior to ischemia and immediately before reperfusion. Serum and samples of ischemic liver lobes were harvested for future analysis, and the 7-day survival rate was assessed after hepatic ischemia-reperfusion surgery.

Results: In comparison with the I/R group, the M + I/R group showed markedly decreased expression levels of inflammatory cytokines (IL-6 and TNF-α) and numbers of apoptotic hepatocytes (P < 0.05). Immunoblotting showed that the expression levels of IL-6, p-NF-κBp65/t-NF-κBp65 and p-IκB-α/t-IκB-α in the M + I/R group were significantly lower than those in the I/R group, and immunofluorescence staining showed that the expression level of p-NF-κBp65 in the M + I/R group was lower than that in the I/R group (P < 0.05). The 7-day survival rates were 20% in the I/R group and 50% in the M + I/R group (P < 0.05).

Conclusions: Melatonin downregulated the activity of the NF-κB signaling pathway in the early and late stages of hepatic ischemia-reperfusion injury, alleviated the inflammatory response, protected the liver from ischemia-reperfusion injury, and increased the survival rate.
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http://dx.doi.org/10.1016/j.hbpd.2021.04.001DOI Listing
April 2021

Serum zinc concentrations and prediabetes and diabetes in the general population.

Biol Trace Elem Res 2021 May 1. Epub 2021 May 1.

Department of Clinical Laboratory, Kunshan Hospital of Traditional Chinese Medicine, No.189, Chaoyang Road, Kunshan, 215300, Jiangsu, China.

The association between serum zinc and diabetes development remains uncertain. We examined the associations between serum zinc and prediabetes and diabetes, and adult participants (≥ 20 years) from the 2011-2016 National Health and Nutrition Examination Survey. Serum zinc concentrations were measured with inductively coupled plasma dynamic reaction cell mass spectrometry. Prediabetes and diabetes were defined through questionnaires and fasting plasma glucose and hemoglobin A levels. Weighted logistic regression and restricted cubic splines were adopted to explore the dose-response relationships between serum zinc concentrations and prediabetes and diabetes. Comparing the highest to lowest quartile of serum zinc concentrations, the multivariable-adjusted odds ratio (95% confidence interval) of prediabetes and diabetes was 3.24 (2.33-4.52, P < 0.01) and 1.64 (1.27-2.11, P < 0.01), respectively. In stratified analysis by sex, the association between serum zinc concentrations and prediabetes was found in both men (3.83 (2.60-5.66), P < 0.01) and women (2.41 (1.51-3.87), P < 0.01), and the association between serum zinc concentrations and diabetes was also found in both men (2.05 (1.34-3.13), P < 0.01) and women (1.65 (1.07-2.56), P = 0.15). The association between serum zinc and prediabetes was consistent in stratified analyses by body mass index and hypertension, and the association with diabetes was also found in subjects with hypertension. Restricted cubic splines showed that the relationships between serum zinc concentrations and prediabetes and diabetes were linear, respectively. In conclusion, serum zinc concentrations were positively associated with prediabetes and diabetes in the general population, and the associations were linear. The findings need to be confirmed by prospective cohort studies.
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http://dx.doi.org/10.1007/s12011-021-02739-6DOI Listing
May 2021

Genome-wide variant-based study of genetic effects with the largest neuroanatomic coverage.

BMC Bioinformatics 2021 Apr 30;22(1):223. Epub 2021 Apr 30.

Computer and Information Science, IUPUI, 723 W Michigan St, Indianapolis, IN, 46202, USA.

Background: Brain image genetics provides enormous opportunities for examining the effects of genetic variations on the brain. Many studies have shown that the structure, function, and abnormality (e.g., those related to Alzheimer's disease) of the brain are heritable. However, which genetic variations contribute to these phenotypic changes is not completely clear. Advances in neuroimaging and genetics have led us to obtain detailed brain anatomy and genome-wide information. These data offer us new opportunities to identify genetic variations such as single nucleotide polymorphisms (SNPs) that affect brain structure. In this paper, we perform a genome-wide variant-based study, and aim to identify top SNPs or SNP sets which have genetic effects with the largest neuroanotomic coverage at both voxel and region-of-interest (ROI) levels. Based on the voxelwise genome-wide association study (GWAS) results, we used the exhaustive search to find the top SNPs or SNP sets that have the largest voxel-based or ROI-based neuroanatomic coverage. For SNP sets with >2 SNPs, we proposed an efficient genetic algorithm to identify top SNP sets that can cover all ROIs or a specific ROI.

Results: We identified an ensemble of top SNPs, SNP-pairs and SNP-sets, whose effects have the largest neuroanatomic coverage. Experimental results on real imaging genetics data show that the proposed genetic algorithm is superior to the exhaustive search in terms of computational time for identifying top SNP-sets.

Conclusions: We proposed and applied an informatics strategy to identify top SNPs, SNP-pairs and SNP-sets that have genetic effects with the largest neuroanatomic coverage. The proposed genetic algorithm offers an efficient solution to accomplish the task, especially for identifying top SNP-sets.
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http://dx.doi.org/10.1186/s12859-021-04145-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086096PMC
April 2021

New insights into hallux valgus by whole exome sequencing study.

Exp Biol Med (Maywood) 2021 Apr 29:15353702211008641. Epub 2021 Apr 29.

Department of Cell Biology, the Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.

The traditional view is that the occurrence and development of hallux valgus (HV) are mainly due to environmental factors. Recent studies have suggested the large contribution of genetic heritability to HV, but it remains elusive about the genetic variants underlying the development of HV. To gain knowledge about the molecular mechanisms of HV pathogenesis by genetic approach, whole exome sequencing studies were performed in 10 individuals (7 affected by HV and 3 unaffected) from three independent families. Specific mutations were found to be related to the pathogenesis of HV and conform to the laws of inheritance. A total of 36 genes with functional candidate single nucleotide variants were identified. Genetic predisposition plays an important role in the development of HV. Interestingly, some of these genes are related to chronic arthritis, such as the complement encoding gene , or are related to long toe or long fingers, such as , , , , and . This study identified rare potentially pathogenic mutations represented by genes related to digital anomalies and chronic arthritis underlying the familial types of HV, which acquired new insights into the genetic and physiological foundations of HV, thereby might improve accurate prevention and drug development for HV.
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http://dx.doi.org/10.1177/15353702211008641DOI Listing
April 2021

Toxic effects of ZnSe/ZnS quantum dots on the reproduction and genotoxiticy of rare minnow (Gobiocypris rarus).

Comp Biochem Physiol C Toxicol Pharmacol 2021 Apr 26;247:109065. Epub 2021 Apr 26.

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Sciences, Chongqing 400715, China. Electronic address:

ZnSe/ZnS quantum dots (QDs) have excellent optical properties, but researchers have not clearly determined whether they cause harm to organisms. In the present study, the effect of ZnSe/ZnS QDs on the parents and offspring of rare minnow were evaluated for the first time. Exposure to ZnSe/ZnS QDs altered the testicular structure, caused sperm DNA damage and decreased sperm motility in males. They also suppressed the expression of reproduction-related genes, such as androgen receptor (Ar), DM-related transcription factor 1 (Dmrt1), estrogen receptor (Er), and X-ray repair cross complementing gene 1 (Xrcc1). Continued monitoring of the F1 generation revealed that the embryonic development of the F1 generation was abnormal and the growth index of the F1 generation of adult fish showed hormesis. A comet assay showed that the F1 generation still had DNA damage in the 400 and 800 nmol/L groups at 96 h post-fertilization (hpf). Thus, ZnSe/ZnS QDs damaged the reproductive system of the rare minnow, and this effect continued to the F1 generation.
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http://dx.doi.org/10.1016/j.cbpc.2021.109065DOI Listing
April 2021

Operating Performance Improvement Based on Prediction and Grade Assessment for Sintering Process.

IEEE Trans Cybern 2021 Apr 28;PP. Epub 2021 Apr 28.

Sintering is the preproduction process of ironmaking, whose products are the basis of ironmaking. How to improve the operating performance of the iron ore sintering process has always been a problem that operators are committed to solve. An operating performance improvement method based on prediction and grade assessment is presented in this article. First, considering the data distribution characteristics of the process, a performance index prediction model based on the Gaussian process regression is built, in which the mutual information analysis method is used to select the inputs of the performance index prediction model. Then, the operating performance grade is assessed by a threshold division method. Next, the operating performance grade guides the control of the burn-through point to improve the operating performance. Finally, experimental verification is performed based on the actual running data. The results show that the proposed method has high prediction accuracy, and it is also significant in improving the operating performance. Therefore, this approach provides an effective solution to predict and improve operating performance.
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http://dx.doi.org/10.1109/TCYB.2021.3071665DOI Listing
April 2021

Functionalization of DNA-Tagged Alkenes Enabled by Visible-Light-Induced C-H Activation of -Aryl Tertiary Amines.

Org Lett 2021 May 28;23(9):3486-3490. Epub 2021 Apr 28.

HitGen Inc., Building 6, No. 8 Huigu First East Road, Tianfu International Bio-Town, Shuangliu District, Chengdu 610200, Sichuan, P. R. China.

A highly efficient approach to C(sp)-C(sp) bond construction via on-DNA photoredox catalysis between on-DNA alkenes and -aryl tertiary amines was developed. The methodology demonstrated 55%-95% conversions without obvious DNA damage, as seen by qPCR tests. Furthermore, various functional groups, such as carboxylic acids, aldehydes, and aryl halides, that can be used to create library diversities were shown to be tolerant of the C-H activation conditions.
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http://dx.doi.org/10.1021/acs.orglett.1c00924DOI Listing
May 2021

Maternal folic acid supplementation prevents autistic behaviors in a rat model induced by prenatal exposure to valproic acid.

Food Funct 2021 Apr 27. Epub 2021 Apr 27.

College of Life Sciences, Shaanxi Normal University. Xi'an, Shaanxi, 710119, China.

Maternal vitamin supplementation has been demonstrated to reduce the risks of a number of neurodevelopmental diseases in children. Autism spectrum disorder (ASD) is a group of neurodevelopment defects with high prevalence but without satisfactory therapy. The present work detected the effects of pregnancy supplementation with folic acid (FA) at different doses on rat models of ASD induced by prenatal exposure to valproic acid (VPA), an anti-epileptic increasing the risk of ASD when administered during pregnancy. The results show that maternal FA supplementation at a high dose (4 mg kg-1) prevented the delay in growth and development, and the deficits in social communicative behaviors and repetitive behaviors, possibly by restoring the increased dendritic spine density and rectifying the over-expression of synaptic proteins associated with excitatory neurons and the lower expression with inhibitory ones. The results provided experimental evidence suggesting a possible role of maternal FA supplementation in preventing ASD.
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http://dx.doi.org/10.1039/d0fo02926bDOI Listing
April 2021

Late Pleistocene partial femora from Maomaodong, southwestern China.

J Hum Evol 2021 Apr 22;155:102977. Epub 2021 Apr 22.

Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Palaeontology and Palaeoanthropology, Chinese Academy of Sciences, Beijing 100044, China; CAS Center for Excellence in Life and Paleoenvironment, Beijing 100044, China.

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http://dx.doi.org/10.1016/j.jhevol.2021.102977DOI Listing
April 2021

Haploinsufficiency in non-homologous end joining factor 1 induces ovarian dysfunction in humans and mice.

J Med Genet 2021 Apr 22. Epub 2021 Apr 22.

Obstetrics and Gynecology Hospital, NHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), State Key Laboratory of Genetic Engineering at School of Life Sciences, Institute of Metabolism and Integrative Biology, Fudan University, Shanghai, China

Background: Premature ovarian insufficiency (POI) is a common disease in women that leads to a reduced reproductive lifespan. The aetiology of POI is genetically heterogeneous, with certain double-strand break (DSB) repair genes being implicated in POI. Although non-homologous end joining (NHEJ) is an efficient DSB repair pathway, the functional relationship between this pathway and POI remains unknown.

Methods And Results: We conducted whole-exome sequencing in a Chinese family and identified a rare heterozygous loss-of-function variant in non-homologous end joining factor 1 (): c.532C>T (p.R178*), which co-segregated with POI and irregular menstruation. The amount of NHEJ1 protein in the proband was half of the normal level, indicating a link between haploinsufficiency and POI. Furthermore, another rare heterozygous variant c.500A>G (p.Y167C) was identified in one of 100 sporadic POI cases. Both variants were predicted to be deleterious by multiple in silico tools. In vitro assays showed that knock-down of in human KGN ovarian cells impaired DNA repair capacity. We also generated a knock-in mouse model with a heterozygous variant equivalent to p.R178* in familial patients. Compared with wild-type mice, heterozygous -mutated female mice required a longer time to first birth, and displayed reduced numbers of primordial and growing follicles. Moreover, these mice exhibited higher sensitivity to DSB-inducing drugs. All these phenotypes are analogous to the progressive loss of ovarian function observed in POI.

Conclusions: Our observations in both humans and mice suggest that haploinsufficiency is associated with non-syndromic POI, providing novel insights into genetic counselling and clinical prevention of POI.
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http://dx.doi.org/10.1136/jmedgenet-2020-107398DOI Listing
April 2021

The relationships of both transition shock, empathy, resilience and coping strategies with professional quality of life in newly graduated nurses.

BMC Nurs 2021 Apr 23;20(1):65. Epub 2021 Apr 23.

Department of Nursing, West China Hospital/West China School of Nursing, Sichuan University, 37, Guoxue Road, Chengdu, Sichuan Province, People's Republic of China.

Background: Data on professional quality of life in newly graduated nurses are scarce. This study aimed to describe the levels of professional quality of life, and to explore the relationships of transition shock, empathy, resilience and coping strategies with professional quality of life in newly graduated nurses.

Methods: This was a cross-sectional study, which used a two-stage sampling method to recruit 393 newly graduated nurses in Sichuan province of China. Multiple regression analysis was used to explore the effects of transition shock, empathy, resilience and coping strategies on professional quality of life. Data were collected using standardized scales.

Results: The prevalence of average levels of compassion satisfaction, burnout and secondary traumatic stress in newly graduated nurses were 80.2, 38.2 and 57.5%, respectively. Transition shock was a significant negative predictor, and empathy, resilience and adaptive coping were significant positive predictors for compassion satisfaction. Transition shock and passive coping were significant positive predictors, and empathy was a significant negative predictor for burnout and secondary traumatic stress. Resilience and adaptive coping contributed to burnout significantly and negatively.

Conclusion: Higher transition shock and lower empathy cause lower compassion satisfaction and higher compassion fatigue. More resilience and adaptive coping cause more compassion satisfaction and less burnout. More passive coping contributes to higher compassion fatigue. Strategies such as transition or preceptorship programmes, and empathy, resilience and coping training are effective methods to reduce transition shock, facilitate empathy, resilience and coping, and consequently, enhance professional quality of life in newly graduated nurses.
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http://dx.doi.org/10.1186/s12912-021-00589-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062214PMC
April 2021

First report of Eimeria and Entamoeba infection in alpacas (Vicugna pacos) in Shanxi Province, northern China.

Parasitol Res 2021 Apr 22. Epub 2021 Apr 22.

College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province, 030801, People's Republic of China.

Intestinal protozoa Eimeria and Entamoeba can infect many animal species including alpacas. However, data on the prevalence and pathogenicity of species of the two genera Eimeria and Entamoeba in alpacas in China is scarce. The current study was carried out to investigate the prevalence of Eimeria and Entamoeba in alpacas in two cities (Taiyuan and Xinzhou) in Shanxi Province, northern China, using PCR-based approaches. Eimeria spp. were only found in Taiyuan city, and the overall prevalence was 1.64%. All samples collected from male alpacas were PCR-negative for Eimeria. Four Eimeria-positive samples were tested positive as Eimeria lamae. The molecular prevalence of Entamoeba in alpacas was 18.03% (66/366), including 16.39% (50/305) in alpacas from Taiyuan city and 26.23% (16/61) from Xinzhou city, respectively. The Entamoeba prevalence in male alpacas (25.00%) was significantly higher than that in female alpacas (15.69%). Entamoeba bovis was the predominant species, and no Entamoeba histolytica infection was detected. Nine unique SSU rRNA gene sequences of Entamoeba were obtained which formed a new cluster. The results showed that sex and location might be the risk factors associated with prevalence of Eimeria spp., and sex might be the risk factor associated with prevalence of Entamoeba spp.. This is the first report of Entamoeba in alpacas worldwide. These findings expand our understanding of the prevalence and genetic diversity of Eimeria and Entamoeba in alpacas.
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http://dx.doi.org/10.1007/s00436-021-07157-0DOI Listing
April 2021

A New Parameter, the Smallest Oblique Sagittal Area of the Neural Foramen, as an Index to Diagnose Cervical Neural Foramen Stenosis.

Can Assoc Radiol J 2021 Apr 19:8465371211005540. Epub 2021 Apr 19.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou City, China.

Objective: The objective was to reconstruct the cervical neural foramen and accurately measure the minimum oblique sagittal area of the neural foramen. Then, a quantitative diagnostic standard for cervical neural foramen stenosis was proposed and its value as an indication for surgery was evaluated.

Methods: (1) CT data were used to reconstruct the neural foramen using Mimics software, and the minimum area was measured. (2) The optimal cut-off value was determined using a receiver operating characteristic (ROC) curve. (3) Patients who underwent single-segment surgery were divided into 2 groups according to the cut-off value. Then the postoperative neurological function improvement rate was analyzed to identify any significant difference between the 2 groups.

Results: A total of 1056 neural foramens were measured in 132 patients, of which 495 (46.88%) were diagnosed as radiculopathy by clinical neurological examination. The optimal cut-off value determined by the ROC curve was 25.95 mm (sensitivity 74.1%, specificity 80.9%) and the area under the curve (AUC) was 0.827 (95%CI: 0.803-0.849). There was a significant difference in the neurological function improvement rate between the 2 groups after surgery ( < 0.05). The intraclass correlation coefficient (ICC) was 0.969.

Conclusion: Three-dimensional digital simulation reconstruction of CT data is a good measurement method. The optimal cut-off value determined here not only has a certain reference value for the diagnosis of cervical neural foramen bony stenosis, but also helps to select patients suitable for neural foramen decompression and can be used as a reference for surgical indication.
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http://dx.doi.org/10.1177/08465371211005540DOI Listing
April 2021

RNA-seq reveals plant virus composition and diversity in alfalfa, thrips, and aphids in Beijing, China.

Arch Virol 2021 Apr 17. Epub 2021 Apr 17.

College of Grassland Science and Technology, China Agricultural University, Beijing, China.

Viruses are widespread in alfalfa (Medicago sativa L.), representing a key limitation to the production of this important forage plant. Understanding the diversity of plant viruses in alfalfa and their potential vectors will play an important role in management to minimize the emergence, transmission, and impact of viruses. Next-generation sequencing (NGS) targeting the transcriptome was applied to monitor the virus communities in alfalfa and its two main pests, thrips (Odontothrips loti Haliday and Frankliniella intonsa Trybom) and aphids (Acyrthosiphon pisum Mordvilko and Therioaphis trifolii Monell). A comparison of transcriptome datasets with reference databases revealed the presence of eight candidate viruses. Five out of the eight viruses, alfalfa mosaic virus (AMV), Medicago sativa alphapartitivirus 1 (MsAPV1), Medicago sativa deltapartitivirus 1 (MsDPV1), Medicago sativa amalgavirus 1 (MsAV1), and bean yellow mosaic virus (BYMV), were confirmed by RT-PCR. We identified and determined the presence of four RNA viruses from alfalfa samples, two viruses (AMV and MsAPV1) from thrips samples, and one virus (BYMV) from T. trifolii. All sequences isolated from the insect samples were more than 95% identical to the sequences from the alfalfa samples or to sequences from the National Center for Biotechnology Information (NCBI) reference database. The RNA-seq results of this study suggest that AMV and MsAPV1 are the predominant RNA plant viruses infecting alfalfa and that they are carried by the major pests. This lays the foundation for future research on the vectors and transmission of these viruses. In addition, the sequence data have enabled the assembly of the first complete genome sequence of MsDPV1 from alfalfa.
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http://dx.doi.org/10.1007/s00705-021-05067-1DOI Listing
April 2021

A multicenter clinical study: personalized medication for advanced gastrointestinal carcinomas with the guidance of patient-derived tumor xenograft (PDTX).

J Cancer Res Clin Oncol 2021 Apr 17. Epub 2021 Apr 17.

Nanjing Personal Oncology Biological Technology Co. Ltd, Nanjing, Jiangsu, China.

Background: Establish patient-derived tumor xenograft (PDTX) from advanced GICs and assess the clinical value and applicability of PDTX for the treatment of advanced gastrointestinal cancers.

Methods: Patients with advanced GICs were enrolled in a registered multi-center clinical study (ChiCTR-OOC-17012731). The performance of PDTX was evaluated by analyzing factors that affect the engraftment rate, comparing the histological consistency between primary tumors and tumorgrafts, examining the concordance between the drug effectiveness in PDTXs and clinical responses, and identifying genetic variants and other factors associated with prognosis.

Results: Thirty-three patients were enrolled in the study with the engraftment rate of 75.8% (25/33). The success of engraftment was independent of age, cancer types, pathological stages of tumors, and particularly sampling methods. Tumorgrafts retained the same histopathological characteristics as primary tumors. Forty-nine regimens involving 28 drugs were tested in seventeen tumorgrafts. The median time for drug testing was 134.5 days. Follow-up information was obtained about 10 regimens from 9 patients. The concordance of drug effectiveness between PDTXs and clinical responses was 100%. The tumor mutation burden (TMB) was correlated with the effectiveness of single drug regimens, while the outgrowth time of tumorgrafts was associated with the effectiveness of combined regimens.

Conclusion: The engraftment rate in advanced GICs was higher than that of other cancers and meets the acceptable standard for applying personalized therapeutic strategies. Tumorgrafts from PDTX kept attributes of the primary tumor. Predictions from PDTX modeling closely agreed with clinical drug responses. PDTX may already be clinically applicable for personalized medication in advanced GICs.
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http://dx.doi.org/10.1007/s00432-021-03639-xDOI Listing
April 2021

Variety of Ordered Patterns in Donor-Acceptor Polymer Semiconductor Films Crystallized from Solution.

ACS Appl Mater Interfaces 2021 Apr 16;13(16):19055-19063. Epub 2021 Apr 16.

Centre for Photonic Devices and Sensors, University of Cambridge, 9 JJ Thomson Avenue, Cambridge CB3 0FA, United Kingdom.

A huge challenge is to control the nucleation of crystallites/aggregates in the solution during polymer film formation to generate desired structures. In this work, we investigate crystallization of P(NDI2OD-T2), a donor-acceptor polymer semiconductor, with controlled solution flow along the contact line between the drying film and solution through a seesaw-like pivoting of samples during polymer drying. By controlling the pivoting frequency/amplitude, various types of line patterns can be observed: (I) an array of fishbone-like stripes oriented in the film-growth direction; (II) the pinning-depinning of contact line (PDCL)-mechanism-defined patterned wires along the contact line; and (III) periodic twined crystalline line pattern oriented in the direction of the contact line. The rich variety of pattern formation observed is attributed to the distinctiveness of the donor-acceptor conjugated polymer structure. The result measured from thin-film transistors made of the generated films/structures showed that the charge mobility of P(NDI2OD-T2) does not change much with the film morphology, which supports recent controversy over the charge-transportation mechanism of some donor-acceptor polymer semiconductors.
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http://dx.doi.org/10.1021/acsami.1c00079DOI Listing
April 2021

Enhancement of Target-Oriented Opinion Words Extraction with Multiview-Trained Machine Reading Comprehension Model.

Comput Intell Neurosci 2021 27;2021:6645871. Epub 2021 Mar 27.

Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.

Target-oriented opinion words extraction (TOWE) seeks to identify opinion expressions oriented to a specific target, and it is a crucial step toward fine-grained opinion mining. Recent neural networks have achieved significant success in this task by building target-aware representations. However, there are still two limitations of these methods that hinder the progress of TOWE. Mainstream approaches typically utilize position indicators to mark the given target, which is a naive strategy and lacks task-specific semantic meaning. Meanwhile, the annotated target-opinion pairs contain rich latent structural knowledge from multiple perspectives, but existing methods only exploit the TOWE view. To tackle these issues, we formulate the TOWE task as a question answering (QA) problem and leverage a machine reading comprehension (MRC) model trained with a multiview paradigm to extract targeted opinions. Specifically, we introduce a template-based pseudo-question generation method and utilize deep attention interaction to build target-aware context representations and extract related opinion words. To take advantage of latent structural correlations, we further cast the opinion-target structure into three distinct yet correlated views and leverage meta-learning to aggregate common knowledge among them to enhance the TOWE task. We evaluate the proposed model on four benchmark datasets, and our method achieves new state-of-the-art results. Extensional experiments have shown that the pipeline method with our approach could surpass existing opinion pair extraction models, including joint methods that are usually believed to work better.
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http://dx.doi.org/10.1155/2021/6645871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024080PMC
March 2021

Rare Intradural Double Draining Veins in a Spinal Dural Arteriovenous Fistula.

Neurology 2021 Apr 15. Epub 2021 Apr 15.

Xiaodong Niu, MD, Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China; Tongyu Wang, MM, Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China; Cong Wu, MD, Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China; Jin Li, MD, Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

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http://dx.doi.org/10.1212/WNL.0000000000011955DOI Listing
April 2021