Publications by authors named "Jin Huang"

1,279 Publications

  • Page 1 of 1

Design and Synthesis of Osthole-based Compounds as Potential Nrf2 Agonists.

Bioorg Med Chem Lett 2022 Jan 12:128547. Epub 2022 Jan 12.

Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address:

A total of 23 compounds based on Osthole skeleton were designed and synthesized. Their agonistic activity for Nrf2 were evaluated by Dual-luciferase Reporter Gene Assay. Most of the compounds showed better activities compared with Osthole, especially O15 and O21. And the median effective concerntration (EC) values was calculated accordingly, both of which showed remarkable activity for Nrf2. The structure activity relationship study indicated that introduction of the structure of stilbene might be beneficial for enhancement of agonistic properties of Osthole, and the position of the substituent may have a greater effect on the activity than the electron-donating/withdrawing ability of the substituent. Mechanism of the action of selected compound O15 was investigated by molecular docking, cellular thermal shift assay and ubiquitination assay, which suggested the reason why O15 exhibited relatively stronger agonistic activity for Nrf2. Compound O15 and O21 both provided novel methods to investigate Osthole-based compounds as Nrf2 agonists.
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http://dx.doi.org/10.1016/j.bmcl.2022.128547DOI Listing
January 2022

Effects of Preoperative Anxiety Status on Haemodynamic Changes during and after Tooth Extraction in Middle-aged and Elderly Patients with Hypertension: a Prospective Repeated-Measures Cohort Study.

Chin J Dent Res 2022 Jan;24(4):267-274

Objective: To investigate the effects of dental anxiety on fluctuations in blood pressure (BP) and heart rate (HR) during tooth extraction in hypertensive patients under local anaesthesia, and how they are influenced by various confounding variables.

Methods: This is a prospective repeated-measures cohort study involving 600 patients successively recruited from Peking University School and Hospital of Stomatology, Beijing, China. BP and HR were repeatedly measured at rest (T0), before anaesthesia (T1), during tooth extraction (T2) and after tooth extraction (T3). Anxiety status was measured prior to local anaesthesia using a modified dental anxiety scale (MDAS). Three groups were assigned: mild anxiety (Corah DAS score of 4 to 8), moderate anxiety (score of 9 to 12) and severe anxiety (score of 13 to 20). We used a generalised linear mixed model (GLMM) to analyse the effects of dental anxiety on fluctuations in BP and HR. Interaction analysis was used to further explore the correlationship between these interactive factors.

Results: The mean anxiety scale score was 9.63 ± 2.88. Severe preoperative anxiety (score of 14 to 20) was associated with significantly increased HR during administration of anaesthesia. Patients with severe anxiety also displayed a significantly greater increase in HR during anaesthetic administration (P < 0.001). When analysing the joint effects of different anxiety statuses over time, blood pressure was significantly elevated in all patients with moderate and severe anxiety during tooth extraction at T2 (β = 1.25, 95% CI 0.24 to 2.27). We also observed a significant decrease in HR in the moderate anxiety group at T3 (β = -1.51, 95% CI -2.38 to -0.63) and a significant increase in HR in the severe anxiety group at T1, T2 and T3 (β = 2.52, 95% CI 1.12 to 3.93; β = 3.84, 95% CI 2.30 to 5.38; β = 4.57, 95% CI 3.03 to 6.11, respectively).

Conclusion: This study indicates that the effects of dental anxiety on BP and HR in middle-aged and elderly patients with hypertension during local anaesthesia and tooth extraction were influenced by various confounding variables.
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http://dx.doi.org/10.3290/j.cjdr.b2440843DOI Listing
January 2022

EndMT: New findings on the origin of myofibroblasts in endometrial fibrosis of intrauterine adhesions.

Reprod Biol Endocrinol 2022 Jan 7;20(1). Epub 2022 Jan 7.

Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China.

Background: Intrauterine adhesion (IUA) is one of the leading causes of infertility and the main clinical challenge is the high recurrence rate. The key to solving this dilemma lies in elucidating the mechanisms of endometrial fibrosis. The aim of our team is to study the mechanism underlying intrauterine adhesion fibrosis and the origin of fibroblasts in the repair of endometrial fibrosis.

Methods: Our experimental study involving an animal model of intrauterine adhesion and detection of fibrosis-related molecules. The levels of molecular factors related to the endothelial-to-mesenchymal transition (EndMT) were examined in a rat model of intrauterine adhesion using immunofluorescence, immunohistochemistry, qPCR and Western blot analyses. Main outcome measures are levels of the endothelial marker CD31 and the mesenchymal markers alpha-smooth muscle actin (α-SMA) and vimentin.

Results: Immunofluorescence co-localization of CD31 and a-SMA showed that 14 days after moulding, double positive cells for CD31 and a-SMA could be clearly observed in the endometrium. Decreased CD31 levels and increased α-SMA and vimentin levels indicate that EndMT is involved in intrauterine adhesion fibrosis.

Conclusions: Endothelial cells promote the emergence of fibroblasts via the EndMT during the endometrial fibrosis of intrauterine adhesions.
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http://dx.doi.org/10.1186/s12958-022-00887-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739974PMC
January 2022

A novel FRET-based dendritic hybridization chain reaction for tumour-related mRNA imaging.

Chem Commun (Camb) 2022 Jan 7. Epub 2022 Jan 7.

State Key Laboratory of Chemo/Biosensing and Chemometrics, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, China.

A novel FRET-based dendritic hybridization chain reaction (D-HCR) for TK1 mRNA imaging in living cells was developed. Compared with traditional complex D-HCR methods, it includes the advantages of having a simple design, an accurate signal and is suitable for use with living cells.
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http://dx.doi.org/10.1039/d1cc06009kDOI Listing
January 2022

Monitoring and Predicting Treatment Response of Extraocular Muscles in Grave's Orbitopathy by Tc-DTPA SPECT/CT.

Front Med (Lausanne) 2021 16;8:791131. Epub 2021 Dec 16.

Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha, China.

To investigate single-photon emission computed tomography/computed tomography (SPECT/CT) for assessing inflammation in the extraocular muscles (EOMs) and predicting the therapeutic efficacy of periocular glucocorticoid therapy (PGT) for Grave's ophthalmopathy (GO). A total of 412 eyes from 206 patients with GO referred for Tc-DTPA orbital SPECT/CT were enrolled. Fourteen age- and gender-matched healthy controls (28 eyes) were included. The thickness and uptake ratio (UR) of four EOMs were derived from SPECT/CT. Eighty-six eyes from patients with GO patients received PGT. Changes in SPECT/CT parameters were evaluated between the pre- and post-treatment. 195 eyes and 217 eyes were classified as active and inactive stages by clinical activity score (CAS). Values of the thickness and UR of each EOM, T, and U were all significantly higher in the active GO than in the inactive GO and controls ( < 0.01). Among the 86 eyes (48 GO patients) included in the efficacy analysis, 56 eyes and 30 eyes were classified as responders and non-responders. Values of thicknesses and UR of each EOM, the maximum thickness (T), and the maximum UR (U) all dropped following PGT in the responders ( < 0.01). Logistic regression analysis identified the U as an independent predictor for the responders ( < 0.01). Moreover, the U demonstrated incremental predictive value over clinical characters and CAS, as evidenced by the improved area under the curve (0.85 vs. 0.78) and global chi-square (34.12 vs. 18.1). Tc-DTPA SPECT/CT has the potential to assess inflammatory activity by detecting the involvement of EOMs in GO. Pre-treatment UR provides independent and incremental values for the prediction of PGT treatment response.
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http://dx.doi.org/10.3389/fmed.2021.791131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716578PMC
December 2021

Discovery of Orally Available Retinoic Acid Receptor-Related Orphan Receptor γ-t/Dihydroorotate Dehydrogenase Dual Inhibitors for the Treatment of Refractory Inflammatory Bowel Disease.

J Med Chem 2022 Jan 27;65(1):592-615. Epub 2021 Dec 27.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.

Inflammatory bowel disease (IBD) is a multifactorial autoimmune disease, representing a major clinical challenge. Herein, a strategy of dual-targeting approach employing retinoic acid receptor-related orphan receptor γ-t (RORγt) and dihydroorotate dehydrogenase (DHODH) was proposed for the treatment of IBD. Dual RORγt/DHODH inhibitors are expected not only to reduce RORγt-driven Th17 cell differentiation but also to mitigate the expansion and activation of T cells, which may enhance anti-inflammatory effects. Starting from 2-aminobenzothiazole hit , a series of 2-aminotetrahydrobenzothiazoles were discovered as potent dual RORγt/DHODH inhibitors. Compound stands out with IC values of 0.110 μM for RORγt and of 0.297 μM for DHODH. With acceptable mouse pharmacokinetic profiles, exhibited remarkable anti-inflammatory activity and dose-dependently alleviated the severity of dextran sulfate sodium (DSS)-induced acute colitis in mice. Taken together, the present study provides a novel framework for the development of therapeutic agents for the treatment of IBD.
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http://dx.doi.org/10.1021/acs.jmedchem.1c01746DOI Listing
January 2022

Tetracycline removal via adsorption and metal-free catalysis with 3D macroscopic N-doped porous carbon nanosheets: Non-radical mechanism and degradation pathway.

J Environ Sci (China) 2022 Jan 13;111:351-366. Epub 2021 May 13.

School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 611731, China. Electronic address:

Recently, metal-based carbon materials have been verified to be an effective persulfate activator, but secondary pollution caused by metal leaching is inevitable. Hence, a green metal-free 3D macroscopic N-doped porous carbon nanosheets (NPCN) was synthesized successfully. The obtained NPCN showed high adsorption capacity of tetracycline (TC) and excellent persulfate (PS) activation ability, especially when calcined at 700 °C (NPCN-700). The maximum adsorption capacity of NPCN-700 was 121.51 mg/g by H-bonds interactions. Moreover, the adsorption process followed pseudo-second-order kinetics model and Langmuir adsorption isotherm. The large specific surface area (365.27 mg/g) and hierarchical porous structure of NPCN-700 reduced the mass transfer resistance and increased the adsorption capacity. About 96.39% of TC was removed after adding PS. The effective adsorption of the catalyst greatly shortened the time for the target organic molecules to migrate to the catalyst. Moreover, the NPCN-700 demonstrated high reusability with the TC removal rate of 80.23% after 4 cycles. Quenching experiment and electron paramagnetic resonance (EPR) test confirmed the non-radical mechanism dominated by O. More importantly, the C = O groups, defects and Graphitic N acted as active sites to generate O. Correspondingly, electrochemical measurement revealed the direct electron transfer pathway of TC degradation. Finally, multiple degradation intermediates were recognized by the LC-MS measurement and three possible degradation pathways were proposed. Overall, the prepared NPCN had excellent application prospects for removal of antibiotics due to its remarkable adsorption and catalytic degradation capabilities.
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http://dx.doi.org/10.1016/j.jes.2021.04.014DOI Listing
January 2022

Photocaged amplified FRET nanoflares: spatiotemporal controllable of mRNA-powered nanomachines for precise and sensitive microRNA imaging in live cells.

Nucleic Acids Res 2021 Dec 22. Epub 2021 Dec 22.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, P.R. China.

There is considerable interest in creating a precise and sensitive strategy for in situ visualizing and profiling intracellular miRNA. Present here is a novel photocaged amplified FRET nanoflare (PAFN), which spatiotemporal controls of mRNA-powered nanomachine for precise and sensitive miRNA imaging in live cells. The PAFN could be activated remotely by light, be triggered by specific low-abundance miRNA and fueled by high-abundance mRNA. It offers high spatiotemporal control over the initial activity of nanomachine at desirable time and site, and a 'one-to-more' ratiometric signal amplification model. The PAFN, an unprecedented design, is quiescent during the delivery process. However, upon reaching the interest tumor site, it can be selectively activated by light, and then be triggered by specific miRNA, avoiding undesirable early activation and reducing nonspecific signals, allowing precise and sensitive detection of specific miRNA in live cells. This strategy may open new avenues for creating spatiotemporally controllable and endogenous molecule-powered nanomachine, facilitating application at biological and medical imaging.
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http://dx.doi.org/10.1093/nar/gkab1258DOI Listing
December 2021

Aromatic glycosides from the aerial part of .

J Asian Nat Prod Res 2021 Dec 22:1-8. Epub 2021 Dec 22.

Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150036, China.

A new aromatic glycoside () and twelve (-) known aromatic glycosides were isolated from the n-butyl alcohol of the 70% EtOH extract of the aerial part of Among them, compounds -, , - were isolated from Umbelliferae plants for the first time. Their structures were elucidated by NMR and MS spectroscopy, and the absolute configuration of compound was elucidated by single-crystal X-ray diffraction. Compounds and showed moderate cytotoxic activities on MGC-803 cell lines with IC values of 8.63 ± 0.08 and 13.65 ± 1.73 μM, respectively.
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http://dx.doi.org/10.1080/10286020.2021.2017897DOI Listing
December 2021

The association of complex genetic background with the prognosis of acute leukemia with ambiguous lineage.

Sci Rep 2021 12 21;11(1):24290. Epub 2021 Dec 21.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095# Jiefang Ave., Wuhan, 430030, People's Republic of China.

Acute leukemia with ambiguous lineage (ALAL) is a rare and highly aggressive malignancy with limited molecular characterization and therapeutic recommendations. In this study, we retrospectively analyzed 1635 acute leukemia cases in our center from January 2012 to June 2018. The diagnose of ALAL was based on either EGIL or 2016 WHO criteria, a total of 39 patients were included. Four patients diagnosed as acute undifferentiated leukemia (AUL) by both classification systems. Among the patients underwent high-throughput sequencing, 89.5% were detected at least one mutation and the median number of gene mutation was 3 (0-8) per sample. The most frequently mutated genes were NRAS (4, 21%), CEBPA (4, 21%), JAK3 (3, 16%), RUNX1 (3, 16%). The mutations detected in mixed-phenotype acute leukemia (MPAL) enriched in genes related to genomic stability and transcriptional regulation; while AUL cases frequently mutated in genes involved in signaling pathway. The survival analysis strongly suggested that mutation burden may play important roles to predict the clinical outcomes of ALAL. In addition, the patients excluded by WHO criteria had even worse clinical outcome than those included. The association of the genetic complexity of blast cells with the clinical outcomes and rationality of the diagnostic criteria of WHO system need to be evaluated by more large-scale prospective clinical studies.
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http://dx.doi.org/10.1038/s41598-021-03709-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692450PMC
December 2021

Recent advances in biosensor for DNA glycosylase activity detection.

Talanta 2022 Mar 15;239:123144. Epub 2021 Dec 15.

State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, China.

Base excision repair (BER) is vital for maintaining the integrity of the genome under oxidative damage. DNA glycosylase initiates the BER pathway recognizes and excises the mismatched substrate base leading to the apurinic/apyrimidinic site generation, and simultaneously breaks the single-strand DNA. As the aberrant activity of DNA glycosylase is associated with numerous diseases, including cancer, immunodeficiency, and atherosclerosis, the detection of DNA glycosylase is significant from bench to bedside. In this review, we summarized novel DNA strategies in the past five years for DNA glycosylase activity detection, which are classified into fluorescence, colorimetric, electrochemical strategies, etc. We also highlight the current limitations and look into the future of DNA glycosylase activity monitoring.
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http://dx.doi.org/10.1016/j.talanta.2021.123144DOI Listing
March 2022

Metric-Driven 3D Frame Field Generation.

IEEE Trans Vis Comput Graph 2021 Dec 17;PP. Epub 2021 Dec 17.

Controlling the size and shear of elements is crucial in pure hex or hex-dominant meshing. To this end, non-orthonormal frame fields that are almost everywhere integrable (except for the singularities) can play a key role. However, it is often challenging or impossible to generate such a frame field under the tight control of a general Riemannian metric field. Therefore, we propose to solve a relatively weaker problem, i.e., generating such a frame field for a Riemannian metric field that is flat away from singularities. Such a metric field admits a local isometry to 3D Euclidean space. Applying Cartans first structural equation to the associated rotation field, i.e., the rotation part of the frame field, we show that the rotation field must have zero covariant derivatives under the 3D connection induced by the metric field. This observation leads to a metric-aware smoothness measure, equivalent to local integrability. The use of such a measure can be justified on meshes associated with locally flat metric fields. We also propose a method to generate smooth metric fields under a few intuitive constraints. On cuboid shapes, our method generates singularities aware of the metric fields, which makes the parameterization match the input metric fields better than the conventional methods. For generic shapes, while our method generates visually similar results to those using boundary frame fields to guide the metric field generation, the integrability and consistency of the metric fields are still improved, as reflected by the statistics.
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http://dx.doi.org/10.1109/TVCG.2021.3136199DOI Listing
December 2021

Correction: Amplification of early drought responses caused by volatile cues emitted from neighboring tea plants.

Hortic Res 2021 Dec 10;8(1):263. Epub 2021 Dec 10.

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, 230036, Hefei, Anhui, P. R. China.

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http://dx.doi.org/10.1038/s41438-021-00708-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664803PMC
December 2021

Facile fabrication of Fe/FeC embedded in N-doped carbon nanofiber for efficient degradation of tetracycline via peroxymonosulfate activation: Role of superoxide radical and singlet oxygen.

J Colloid Interface Sci 2022 Mar 30;609:86-101. Epub 2021 Nov 30.

School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 611731, China; Research Branch of Advanced Functional Materials, School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 610054, China.

The toxic metal ions leaching and metal nanoparticles agglomeration were the critical issues for metal-based carbon materials during the peroxymonosulfate (PMS) activation processes. Herein, a facile strategy was first proposed that zero-dimensional Fe/FeC nanoparticles were embedded in one-dimensional N-doped carbon nanofiber (Fe/[email protected]) to solve the above challenges. The as-obtained Fe/[email protected] possessed a low E value (11.7 kJ/mol) and exhibited high activity for activating PMS to degrade tetracycline (TC) in a wide range of pH 3-11. As expected, the iron ions leaching concentration of Fe/[email protected] was very low (0.082 mg/L). Meanwhile, the Fe/[email protected] was easily recovered from the reaction solution due to its magnetic properties. Both superoxide radicals (O) and non-radical of singlet oxygen (O) were the primary reactive oxygen species (ROS) in the Fe/[email protected]/PMS system via quenching tests and electron spin resonance spectroscopy (ESR). The catalytic mechanism suggested that the Fe/FeC and graphitic N were the main active sites in the Fe/[email protected] for PMS activation. This work provided a facile method for the preparation of Fe-based carbon materials with high catalytic ability, low metal leaching and easy recycling, showing a broad prospect for environmental applications.
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http://dx.doi.org/10.1016/j.jcis.2021.11.178DOI Listing
March 2022

Detecting Air Pollutant Molecules Using Tube-Shaped Single Electron Transistor.

Molecules 2021 Nov 24;26(23). Epub 2021 Nov 24.

Key Laboratory of Extraordinary Bond Engineering and Advanced Materials Technology of Chongqing, Yangtze Normal University, Chongqing 408100, China.

An air pollution detector is proposed based on a tube-shaped single-electron transistor (SET) sensor. By monitoring the flow control component of the detector, each air pollutant molecule can be placed at the center of a SET nanopore and is treated as an island of the SET device in the same framework. Electron transport in the SET was incoherent, and the performances of the SET were sensitive at the single molecule level. Employing first-principles calculations, electronic features of an air pollutant molecule within a tube-shaped SET environment were found to be independent of the molecule rotational orientations with respect to axis of symmetry, unlike the electronic features in a conventional SET environment. Charge stability diagrams of the island molecules were demonstrated to be distinct for each molecule, and thus they can serve as electronic fingerprints for detection. Using the same setup, quantification of the air pollutant can be realized at room temperature as well. The results presented herein may help provide guidance for the identification and quantification of various types of air pollutants at the molecular level by treating the molecule as the island of the SET component in the proposed detector.
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http://dx.doi.org/10.3390/molecules26237098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658781PMC
November 2021

Correction: Shao et al. AKT Axis, miR-21, and RECK Play Pivotal Roles in Dihydroartemisinin Killing Malignant Glioma Cells. 2017, , 350.

Int J Mol Sci 2021 Nov 24;22(23). Epub 2021 Nov 24.

Institute of Life Sciences, Chongqing Medical University, 1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China.

The authors wish to make the following corrections to this paper [...].
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http://dx.doi.org/10.3390/ijms222312670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8657491PMC
November 2021

Unveiling the boosting of metal organic cage leaching substance on the electrocatalytic oxygen evolution reaction.

J Colloid Interface Sci 2022 Mar 27;610:1035-1042. Epub 2021 Nov 27.

School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, PR China; School of Pharmacy, Guangxi Medical University, Nanning 530021, PR China. Electronic address:

Catalysts often undergo changes during the process of catalytic reactions, which makes the whole catalytic reaction system complicated and brings about much difficulty for the exploration of catalytic mechanism. Herein, we report that an octahedral metal organic cage (MOC) with stress was directionally transformed into two-dimensional nanoarrays maintaining the structure of precursor and new soluble low-nuclear complexes during the electrocatalytic oxygen evolution reaction (OER). The in-situ generated miscible electrocatalyst exhibits an overpotential as low as 197 mV at 10 mA cm, with a high electrochemical stability up to 5 h. Notably, the miscible catalyst can be used as bifunctional electrocatalyst for OER and hydrogen evolution reaction (HER) and exhibits an ultra-low overpotential of 293 mV, even achieve overall water splitting under the voltage provided by a 1.5 V AA battery. As revealed by density functional theory simulations, the position of SO in MOC heterogeneous catalyst is regulated by the soluble low-nuclear complexes to reduce the activation energy of the reaction, leading to an optimization of the OER activity for the reaction system. This work provides a new strategy for the rational design of high-efficiency electrocatalytic system.
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http://dx.doi.org/10.1016/j.jcis.2021.11.150DOI Listing
March 2022

Enhanced methane production coupled with livestock wastewater treatment using anaerobic membrane bioreactor: Performance and membrane filtration properties.

Bioresour Technol 2021 Dec 2;345:126470. Epub 2021 Dec 2.

Department of Environmental Engineering, School of Architecture and Civil Engineering, Chengdu University, Chengdu 610106, China. Electronic address:

The present study introduced a new method for enhanced biomethane production and pollution control of swine wastewater (SW) using anaerobic membrane bioreactor (AnMBR). Results confirmed 35 °C as the optimum temperature for enhanced anaerobic digestion which resulted in relatively higher methane production rate and potential. In AnMBR system, robust pollutants removal and conversion rate were achieved under various hydraulic retention time (HRT) ranging from 20 to 10 days, while the highest methane yield (0.24 L/g-COD) and microbial activity (6.65 mg-COD/g-VSS·h) were recorded at HRT of 15 days. Reduction of HRT to 10 days resulted in serious membrane fouling due to accumulation of extracellularpolymericsubstances(EPS) and cake layer on the membrane. However, cake layer as the dominant membrane foulant could be effectively removed through periodic physical backwash to recover the membrane permeability. Overall, the suggested AnMBR is a promising technology to enhance SW treatment and energy recovery.
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http://dx.doi.org/10.1016/j.biortech.2021.126470DOI Listing
December 2021

First-line pembrolizumab plus chemotherapy for extensive-stage small-cell lung cancer: a United States-based cost-effectiveness analysis.

Cost Eff Resour Alloc 2021 Dec 4;19(1):77. Epub 2021 Dec 4.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: The clinical trial of Keynote-604 showed that pembrolizumab plus chemotherapy could generate clinical benefits for extensive-stage small-cell lung cancer (ES-SCLC). We aim to assess the efficacy and cost of pembrolizumab combined with chemotherapy in the first-line treatment setting of ES-SCLC from the United States (US) payers' perspective.

Methods: A synthetical Markov model was used to evaluate cost and effectiveness of pembrolizumab plus platinum-etoposide(EP) versus EP in first-line therapy for ES-SCLC from the data of Keynote-604. Lifetime costs life-years(LYs), quality adjusted LYs(QALYs) and incremental cost-effectiveness ratios(ICERs) were estimated. One-way and probabilistic sensitivity analyses were performed. Furthermore, we performed subgroup analysis.

Results: Pembrolizumab plus EP resulted in additional 0.18 QALYs(0.32 LYs) and corresponding incremental costs $113,625, resulting an ICER of $647,509 per QALY versus EP. The price of pembrolizumab had a significant impact on ICER. Probabilistic sensitivity analysis indicated that pembrolizumab combined chemotherapy may become a cost-effective option with a probability of 0%. Besides, subgroup analysis suggested that all subgroups were not cost-effective.

Conclusion: From the perspective of the US payer, pembrolizumab plus EP is not a cost-effective option for first-line treatment patients with ES-SCLC at a WTP threshold of $150,000 per QALY.
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http://dx.doi.org/10.1186/s12962-021-00329-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642846PMC
December 2021

Combination of HLA-DR on -Specific Cells and Tuberculosis Antigen/Phytohemagglutinin Ratio for Discriminating Active Tuberculosis From Latent Tuberculosis Infection.

Front Immunol 2021 11;12:761209. Epub 2021 Nov 11.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Novel approaches for tuberculosis (TB) diagnosis, especially for distinguishing active TB (ATB) from latent TB infection (LTBI), are urgently warranted. The present study aims to determine whether the combination of HLA-DR on (MTB)-specific cells and TB antigen/phytohemagglutinin (TBAg/PHA) ratio could facilitate MTB infection status discrimination.

Methods: Between June 2020 and June 2021, participants with ATB and LTBI were recruited from Tongji Hospital (Qiaokou cohort) and Sino-French New City Hospital (Caidian cohort), respectively. The detection of HLA-DR on MTB-specific cells upon TB antigen stimulation and T-SPOT assay were simultaneously performed on all subjects.

Results: A total of 116 (54 ATB and 62 LTBI) and another 84 (43 ATB and 41 LTBI) cases were respectively enrolled from Qiaokou cohort and Caidian cohort. Both HLA-DR on IFN-γTNF-α cells and TBAg/PHA ratio showed discriminatory value in distinguishing between ATB and LTBI. Receiver operator characteristic (ROC) curve analysis showed that HLA-DR on IFN-γTNF-α cells produced an area under the ROC curve (AUC) of 0.886. Besides, TBAg/PHA ratio yield an AUC of 0.736. Furthermore, the combination of these two indicators resulted in the accurate discrimination with an AUC of 0.937. When the threshold was set as 0.36, the diagnostic model could differentiate ATB from LTBI with a sensitivity of 92.00% and a specificity of 81.82%. The performance obtained in Qiaokou cohort was further validated in Caidian cohort.

Conclusions: The combination of HLA-DR on MTB-specific cells and TBAg/PHA ratio could serve as a robust tool to determine TB disease states.
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http://dx.doi.org/10.3389/fimmu.2021.761209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632229PMC
November 2021

The Bactericidal Efficacy and the Mechanism of Action of Slightly Acidic Electrolyzed Water on Survival.

Foods 2021 Nov 3;10(11). Epub 2021 Nov 3.

College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China.

In the present work, the bactericidal efficacy and mechanism of slightly acidic electrolyzed water (SAEW) on were evaluated. The results showed that the strains of were killed completely within 30 s by SAEW whose available chlorine concentration (ACC) was higher than 12 mg/L, and it was confirmed that ACC is the main factor affecting the disinfection efficacy of SAEW. Moreover, our results demonstrated that SAEW could destroy the cell membrane of , which was observed by SEM and FT-IR, thus resulting in the leakage of intracellular substances including electrolyte, protein and nucleic acid, and DNA damage. On the other hand, the results found that SAEW could disrupt the intracellular ROS balance of by inhibiting the antioxidant enzyme activity, thus promoting the death of In conclusion, the bactericidal mechanism of SAEW on was explained from two aspects including the damage of the cell membrane and the breaking of ROS balance.
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http://dx.doi.org/10.3390/foods10112671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8621911PMC
November 2021

China's challenge in promoting older migrants' health and wellbeing: a productive ageing perspective.

BMJ 2021 11 24;375:n2874. Epub 2021 Nov 24.

Saint Louis University, St Louis, MO, USA.

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http://dx.doi.org/10.1136/bmj.n2874DOI Listing
November 2021

HLA-DR Expression Level in CD8 T Cells Correlates With the Severity of Children With Acute Infectious Mononucleosis.

Front Immunol 2021 3;12:753290. Epub 2021 Nov 3.

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: This study aimed to assess the host immune signatures associated with EBV infection and its clinical value in indicating the severity of children with acute infectious mononucleosis (IM).

Methods: Twenty-eight pediatric patients with IM aged 3-8 years were enrolled. The immune phenotypes and cytokine secretion capability of T cells were detected.

Results: The percentages and absolute numbers of CD3 and CD8 T cells were significantly increased in IM patients compared with HCs. The percentages of Naïve CD4 and CD8 T cells were decreased but with increased percentages of memory CD4 and CD8 T subsets. Our results showed the upregulation of active marker HLA-DR, TCR-αβ, and inhibitory receptors PD-1, TIGIT in CD8 T cells from IM patients, which suggested that effective cytotoxic T cells were highly against EBV infection. However, EBV exposure impaired the cytokine (IFN-γ, IL-2, and TNF-α) secretion capability of CD4 and CD8 T cells after stimulation with PMA/ionomycin . Multivariate analysis revealed that the percentage of HLA-DR CD8 T cells was an independent prognostic marker for IM. The percentage of HLA-DR CD8 T cells was significantly correlated with high viral load and abnormal liver function results.

Conclusion: Robust expansion and upregulation of HLA-DR in CD8 T cells, accompanied with impaired cytokine secretion, were typical characteristics of children with acute IM. The percentage of HLA-DR CD8 T cells might be used as a prominent marker not only for the early diagnosis but also for indicating the severity of IM.
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http://dx.doi.org/10.3389/fimmu.2021.753290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8596082PMC
November 2021

Rapid Analysis of Visual Receptive Fields by Iterative Tomography.

eNeuro 2021 Nov-Dec;8(6). Epub 2021 Dec 8.

Save Sight Institute, The University of Sydney, Sydney, New South Wales 2000, Australia

Many receptive fields in the early visual system show standard (center-surround) structure and can be analyzed using simple drifting patterns and a difference-of-Gaussians (DoG) model, which treats the receptive field as a linear filter of the visual image. But many other receptive fields show nonlinear properties such as selectivity for direction of movement. Such receptive fields are typically studied using discrete stimuli (moving or flashed bars and edges) and are modelled according to the features of the visual image to which they are most sensitive. Here, we harness recent advances in tomographic image analysis to characterize rapidly and simultaneously both the linear and nonlinear components of visual receptive fields. Spiking and intracellular voltage potential responses to briefly flashed bars are analyzed using non-negative matrix factorization (NNMF) and iterative reconstruction tomography (IRT). The method yields high-resolution receptive field maps of individual neurons and neuron ensembles in primate (marmoset, both sexes) lateral geniculate and rodent (mouse, male) retina. We show that the first two IRT components correspond to DoG-equivalent center and surround of standard [magnocellular (M) and parvocellular (P)] receptive fields in primate geniculate. The first two IRT components also reveal the spatiotemporal receptive field structure of nonstandard (on/off-rectifying) receptive fields. In rodent retina we combine NNMF-IRT with patch-clamp recording and dye injection to directly map spatial receptive fields to the underlying anatomy of retinal output neurons. We conclude that NNMF-IRT provides a rapid and flexible framework for study of receptive fields in the early visual system.
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http://dx.doi.org/10.1523/ENEURO.0046-21.2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8658541PMC
December 2021

Fishing line assisted endoscopic placement of multiple plastic biliary stents for unresectable malignant hilar biliary obstruction: a retrospective study.

BMC Gastroenterol 2021 Nov 19;21(1):435. Epub 2021 Nov 19.

Department of Gastroenterology, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, No. 68, Gehu Middle Road, Wujin District, Changzhou City, 213100, Jiangsu Province, China.

Background And Aims: Stent migration is one of the most common complications during the placement of multiple plastic biliary stents (MPBS) under endoscopy. This study aims to evaluate the feasibility and efficiency of the fishing line assisted (FLA) method for preventing the complication.

Methods: Patients with unresectable malignant hilar biliary obstruction (MHBO) who undergone endoscopic placement of MPBS using the FLA or conventional method from May 2018 to April 2021 in our center were enrolled in the study. The endpoints of this study were the stent migration rate, technical success rates, adverse events rates, times of stent migration, and the procedure time.

Results: FLA group (N = 19) and conventional group (N = 22) had similar baseline characteristics of the patients. The technical success rates (100% vs. 95.5%; P > 0.05), ERCP-related adverse events rates (5.3% vs. 4.5%; P > 0.05), and the stent-related adverse events rates (0% vs. 4.5%; P > 0.05) were no significant differences between the FLA and conventional groups. MPBS inserted using the conventional method consumed more time (median, 33.9 min vs. 15.6 min; P < 0.05) method and increased the times of stent migration (median, 3 times vs. 0 times; P < 0.05) than using the FLA method. Even if no statistical difference was detected in the stent migration rate between groups, this rate was lower in the FLA group than the conventional group (0% vs. 13.6%; P > 0.05).

Conclusions: FLA method is an effective technique for MPBS implantation to prevent stent migration during endoscopic retrograde cholangiography (ERCP). The method should be applied to patients with unresectable MHBO who need to place MPBS.
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http://dx.doi.org/10.1186/s12876-021-02014-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8603567PMC
November 2021

Fluorescence and visual immunoassay of HIV-1 p24 antigen in clinical samples via multiple selective recognitions of CdTe QDs.

Mikrochim Acta 2021 11 17;188(12):422. Epub 2021 Nov 17.

Department of Laboratory Medicine, Department of Dermatology, State Key Laboratory of Biotherapy and Cancer Center, Med+X Center for Manufacturing, Laboratory of Ethnopharmacology, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.

Human immunodeficiency virus (HIV) infection inflicts significant economic and social burdens on many countries worldwide. Given the substantial morbidity and mortality from HIV infection, there is an urgent need for accurate and early detection of the virus. In this study, immunofluorescence and visual techniques are described that detect the HIV-1 p24 antigen, which relied on selective recognition of Ag/Ag nanoparticles (Ag NPs) and Cu/Cu using cadmium telluride quantum dots (CdTe QDs). After the sandwich immunoreactions were accomplished, the alkaline phosphatase (ALP) hydrolyzed L-ascorbic acid 2-phosphate (AAP) to form ascorbic acid (AA) that further reduces Ag and Cu to Ag NPs and Cu, respectively. This method was highly sensitive and selective and could detect as low as 1 pg/mL of p24 antigen by naked eyes and had a good linearity in the concentration range 1-100 pg/mL. When using Ag and Cu as media, the limit of detection (LOD) of the new method was 0.3 pg/mL and 0.2 pg/mL, respectively. Compared with clinical electrochemiluminescence immunoassay (ECLIA) results and clinical data, this method demonstrated good consistency for the quantification of HIV-1 p24 antigen in 34 clinical serum samples. In addition, this method could accurately distinguish HIV from other viruses and infections such as hepatitis B virus, systemic lupus erythematosus, hepatitis C virus, Epstein-Barr virus, cytomegalovirus, lipemia, and hemolysis. Therefore, our dual-mode analysis method may provide additional solutions to identify clinical HIV infection. An immunofluorescence and visualization dual-mode strategy for the detection of p24 antigen was constructed based on immune recognition reaction and a phenomenon that cadmium telluride quantum dots (CdTe QDs) can selectively recognize Ag/Ag nanoparticles (Ag NPs) and Cu/Cu.
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http://dx.doi.org/10.1007/s00604-021-05075-7DOI Listing
November 2021

Amplification of early drought responses caused by volatile cues emitted from neighboring plants.

Hortic Res 2021 Nov 15;8(1):243. Epub 2021 Nov 15.

State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, 230036, Hefei, Anhui, P. R. China.

Plants have developed sophisticated mechanisms to survive in dynamic environments. Plants can communicate via volatile organic compounds (VOCs) to warn neighboring plants of threats. In most cases, VOCs act as positive regulators of plant defense. However, the communication and role of volatiles in response to drought stress are poorly understood. Here, we showed that tea plants release numerous VOCs. Among them, methyl salicylate (MeSA), benzyl alcohol, and phenethyl alcohol markedly increased under drought stress. Interestingly, further experiments revealed that drought-induced MeSA lowered the abscisic acid (ABA) content in neighboring plants by reducing 9-cis-epoxycarotenoid dioxygenase (NCED) gene expression, resulting in inhibition of stomatal closure and ultimately decreasing early drought tolerance in neighboring plants. Exogenous application of ABA reduced the wilting of tea plants caused by MeSA exposure. Exposure of Nicotiana benthamiana to MeSA also led to severe wilting, indicating that the ability of drought-induced MeSA to reduce early drought tolerance in neighboring plants may be conserved in other plant species. Taken together, these results provide evidence that drought-induced volatiles can reduce early drought tolerance in neighboring plants and lay a novel theoretical foundation for optimizing plant density and spacing.
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http://dx.doi.org/10.1038/s41438-021-00704-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8593122PMC
November 2021

Acute care utilization risk among older adults living undiagnosed or unaware of dementia.

J Am Geriatr Soc 2021 Nov 13. Epub 2021 Nov 13.

Division of Geriatric Medicine and Gerontology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Background: Dementia is associated with increased risk of hospitalization and emergency department (ED) visits. Many persons with dementia are undiagnosed or unaware of their diagnosis, however. Our objective was to determine whether undiagnosed dementia or unawareness affects risk of hospitalization or ED visits.

Methods: Retrospective longitudinal cohort study of 3537 community-living adults age ≥65 enrolled in the 2011-2017 National Health and Aging Trends Study with linked fee-for-service Medicare claims. Using self or proxy reported diagnosis, proxy dementia screening questionnaire, cognitive testing, and Medicare claims diagnosis, participants were classified as having (1) no dementia or dementia, for which they were classified as (2) undiagnosed, (3) diagnosed but unaware, or (4) diagnosed and aware. Proportional hazards models evaluated all-cause and potentially preventable hospitalization and ED visit risk by time-varying dementia status, adjusting for older adult characteristics.

Results: Most participants (n = 2879) had no dementia at baseline. Among participants with dementia at baseline (n = 658), 187 were undiagnosed, 300 diagnosed but unaware, and 171 diagnosed and aware. In multivariable adjusted proportional hazards models, persons with undiagnosed dementia had lower risk of hospitalization and ED visits compared to persons diagnosed and aware (all-cause hospitalization aHR 0.59 [0.44, 0.79] and ED visit aHR 0.63 [0.47, 0.85]) and similar risks of these outcomes compared to persons without dementia. Individuals diagnosed but unaware had greater risk compared to those without dementia: aHR 1.37 (1.18, 1.59) for all-cause hospitalization and 1.48 (1.28, 1.71) for ED visits; they experienced risk comparable to individuals diagnosed and aware.

Conclusion: Older adults with undiagnosed dementia are not at increased risk of acute care utilization after accounting for differences in other characteristics. Individuals unaware of diagnosed dementia demonstrate risk similar to individuals aware of the diagnosis. Increasing diagnosis alone may not affect acute care utilization. The role of awareness warrants further investigation.
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http://dx.doi.org/10.1111/jgs.17550DOI Listing
November 2021

Development and Validation of an Autophagy-Related Gene Signature for Predicting the Prognosis of Hepatocellular Carcinoma.

Biomed Res Int 2021 28;2021:7771037. Epub 2021 Oct 28.

Department of Emergency Surgery, Department of Emergency Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.

Purpose: Autophagy is a lysosomal degradation pathway that is essential for maintaining the homeostasis of the intracellular environment. Mounting evidence indicates that autophagy plays an essential role in the occurrence and development of hepatocellular cancer (HCC). This research is aimed at exploring the prognostic value of autophagy-related genes (ARGs) in HCC patients.

Methods: The Wilcoxon test was used to identify differentially expressed ARGs in The Cancer Genome Atlas (TCGA) HCC cohort. Then, the TCGA cohort was randomly divided into training and testing groups. Cox and LASSO regression models were used to screen for autophagy-related genes that affect overall survival (OS) in the TCGA training group. Based on the coefficient of risk genes, we constructed an autophagy-related gene signature for predicting the prognosis of HCC patients. Finally, we validated the prognostic significance of autophagy-related gene signature using the TCGA testing group and three external datasets.

Results: ATG10, BIRC5, GAPDH, and TMEM74 are risk genes for OS. According to the optimal cutoff value of risk score in each HCC dataset, HCC patients can divide into high- and low-risk groups. ARG risk score can significantly distinguish HCC patients with different survival outcomes. Meanwhile, the ARG risk score is independently correlated with OS in multiple HCC cohorts.

Conclusions: The autophagy-related risk score can effectively screen high-risk HCC patients and provide guidance for clinical prevention and treatment of HCC.
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http://dx.doi.org/10.1155/2021/7771037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568514PMC
October 2021

N, S co-doped magnetic mesoporous carbon nanosheets for activating peroxymonosulfate to rapidly degrade tetracycline: Synergistic effect and mechanism.

J Hazard Mater 2022 Feb 22;424(Pt C):127569. Epub 2021 Oct 22.

School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 611731, PR China. Electronic address:

Heteroatoms doped carbon materials are widely used in the advanced oxidation process (AOPs) to remove organic pollutants in water due to the synergies effect between different heteroatoms. In this study, a novel kind of N, S co-doped magnetic mesoporous carbon nanosheets ([email protected]) was prepared by simple one-step pyrolysis. Further, the influence of doping amount of S (L-methionine) and N (melamine) on catalytic activity was studied, the optimized sample [email protected]/PMS showed a satisfying degradation ( 91.07%) for high concentrations of tetracycline (80 mg/L TC) in 10 min, which was attributed to the proper ratio of S content to N content (S(at.%)/ N(at.%)= 0.2097) in the sample could better play its synergistic effect by XPS analysis. The [email protected]/ PMS system also exhibited satisfactory degradation effects in a wide pH range (3-10) and the existence of inorganic ions and humic acid. Then, the degradation mechanisms were mainly through the non-radical pathway (O and electron transfer) and the major active sites were pyridinic N compared to thiophene S, CO, and Fe-N. This study could inspire the design of high-performance active and low-cost heteroatomic doping nano-magnetic catalysts for PMS-based waste treatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127569DOI Listing
February 2022
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